Vol. 11 No. 2 (2005)
Articles

Micropropagation of Rudbeckia hirta L. from seedling explants

Published May 18, 2005
P. Szarvas
University of Debrecen, Centre of Agricultural Sciences, Department of Vegetable Production, Otto Orsós Laboratory, 4032 Debrecen, Böszörményi út 138, Hungary
A. Zsila-André
University of Debrecen, Centre of Agricultural Sciences, Department of Vegetable Production, Otto Orsós Laboratory, 4032 Debrecen, Böszörményi út 138, Hungary
Z. Kováts
Fruit Growing and Ornamental Plant Research and Development Non-profit Company, Budapest, Hungary
M. G. Fári
University of Debrecen, Centre of Agricultural Sciences, Department of Vegetable Production, Otto Orsós Laboratory, 4032 Debrecen, Böszörményi út 138, Hungary
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APA

Szarvas, P., Zsila-André, A., Kováts, Z., & Fári, M. G. (2005). Micropropagation of Rudbeckia hirta L. from seedling explants. International Journal of Horticultural Science, 11(2), 105-108. https://doi.org/10.31421/IJHS/11/2/588

We conducted experiments for developing an in vitro micropropagation protocol starting from meristems of Rudbeckia hirta L seedlings. We pre-soaked the seeds in sterile ion-exchanged water for 17 hours, and then achieved surface disinfection in two separate steps. First, we used concentrated household sodium-hypochloride solution for 20 minutes and, also for 20 minutes, we applied hydrogen peroxide of 10%, which was followed by washing with sterile ion-exchanged water three times. For the propagation of seedling meristems, the combination of half-strenght solid Murashige and Skoog (1962) culture medium containing 10 mg/1 of kinetin or 2 mg/I of kinetin + 0.1 mg/1 of 2iP proved to be the most suitable. The average number of shoot-buds developed from the seedling axillary meristem in the best culture media varied between 5 and 17. Without separating them, we inoculated the shoot-bud clusters on MS culture medium containing 2 mg/1 of IAA. After four weeks of incubation we obtained elongated shoots which we separated and inoculated into a new culture medium and we obtained elongated roots. The rooted plants were gradually acclimatised in the cultivation room, potted and carried to a greenhouse, and then planted in open field for subsequent observation. By adopting this method, our laboratory started the micropropagation of the superior and/or elite genotypes of the Rudbeckia hirta L. being of special value in respect of breeding.

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