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Pollen viability of ‘Besztercei plum' clones depending on the effect of the year
115-121.Views:162The percentage of dark staining pollen grains was higher in spring of 1996 than in the previous year. Data in 1998 resemble those of 1995, concerning the large amount of medium staining pollen grains in the majority of clones. Some clones produced excellent quality pollen also in the third year. whereas there were significant differences in other clones in various years.
The warmer February-March period in 1995 induced an early blooming, and frost affected the orchard not only in winter months, but also immediately before and during blooming. Thus, frost was the possible cause of weaker quality pollen this year. In 1996 warming began a bit late, but it was not broken by drastic falls in temperature, except for the middle of April, when a smaller frost affected the orchard. It is likely that this frost did not influence pollen quality of `Besztercei' and 'Early Besztercei' plum clones significantly. In 1998 warming was continuous and steady, the orchard was not affected by frost immediately before blooming. In March, however, there was frost almost every day, according to daily minimum temperatures.
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Evaluation of biological control option for Bagrada bug (Bagrada hilaris (Burmeister)) in Kenya
43-47.Views:1Brassica production is important for economic development of Kenya. Bagrada bug, a significant pest of brassicas, affects their yields and quality, currently posing a threat to both local and commercial vegetable production in Kenya. Biological control of bagrada bug using natural enemies is a cheaper and environmentally friendly method. The study aimed to identify native egg parasitoid species in Kenya. A field prospection survey of the bagrada bug egg parasitoid was done by a series of bagrada bug egg exposure in different parts of the country. Freshly laid bagrada bug eggs in cards were exposed for possible parasitism in the field for three days. The eggs were later carried to the laboratory at NSRC to await hatching. Two egg parasitoid species Trissolcus basalis (Hymenoptera, Scelionidae) and Gryon sp. were identified during the study after a period of bagrada bug egg exposure in Machakos and Kisumu. Two cards with parasitized bagrada bug eggs were recovered from Machakos from which one card yielded four parasitoids of one species Trissolcus basalis and the other yielded two parasitoids Trissolcus basalis and Gryon sp. One card with two parasitized eggs by Trissolcus basalis was recovered from Kisumu, however, there were no parasitoids identified in Nanyuki, Naivasha and Kitengela where bagrada bug is also prevalent. Results showed parasitoid presence in fields with high bug populations compared to areas with few or no Bagrada bug infestations. Conducting trials in both laboratory and field settings is recommended to obtain clear data on the effectiveness of the identified egg parasitoid in managing the bagrada bug population.
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Studies on the drought responses of apple trees (Malus domestica Borkh.) grafted on different rootstocks
29-36.Views:524The morphological and chemical changes in the leaves of eight apple varieties grafted on M4, M26 and MM 106 rootstocks were examined during fruit development in non-irrigated plantation. The aim of the experiment was to examine the defensive mechanisms in apple trees under the mild and serious dryness occurring during fruit development. The content of a relative chlorophyll (SPA D value) of the apple leaves on eastern side of the trees was lower than western and southern sides under drought occurring du ring fruit devclop111ent Under sustained drought from first of July to August the SPAD value, the weight of leaves were larger and the stomata density was significantly larger than in the previous periods. There was a close positive significant correlations bet ween SPAD and stoma density both in the group with less sensitive to drought (r=0,8429) and in the large water demanded group (r=0,9209) equally. The rate of increase in SPAD values and the stoma density was slower in the group with drought tolerant, than in the group required good water supply. The varieties being sensitive to water deficite such as Akane. Pink Lady and Red Rome van Well have a l ready responded intensively to short time of drought by the increasing of carbohydrate and antioxidants product ions. At 42 days of sustained drought as soon as the level of carbohydrate in the leaves decreased, the antioxidant s contents of the leaves rised suddenly in the apple varieties with moderate water requirement s as Greensleeves and ldared. Gala apple variety seemed to be able to defend the drought for longer time because there was no change in relatively high level of carbohydrates and antioxidants of their leaves under severe drought during fruit development.
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Studies on the alkaloid production of genetically transformed and non-transformed cultures of Lobelia inflata L.
65-71.Views:142The investigations of the growth and alkaloid production of cell suspension-, callus-, organized- and hairy root cultures from Lobelia inflata L. proved that these cultures are able to synthesize the characteristic piperidine alkaloids of the intact plant. Alkaloid precursor amino acids (Phe, Lys) and plant growth regulators affect not only the growth and differentiation of tissue cultures but also their secondary metabolism. The synthetic regulator Sz/I I combined with Phe increased the total alkaloid content considerably in callus- and organized cultures; regulator Sz/28 especially increased the lobeline content (in organized cultures in response to Lys, in callus tissues as a result of Phe application). With the aim of optimizing growth and alkaloid production of the genetically transformed hairy root cultures of Lobelia inflata L. we studied the effect of some growth regulators (NAA, IAA, kinetin) and precursor amino acids (Lys, Phe). The kinetin had inhibiting effect on the growth and lobeline production of the hairy roots. The IAA and NAA increased the biomass formation and lobeline production. The highest lobeline level was detected in tissues cultivated on hormone-free medium containing Phe.
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Relationship between several meteorological factors and phenological features of pear cultivars
67-73.Views:211The aim of this paper was to investigate the fl owering characteristic of apples and their relationship to meteorological parameters. The trees observed are grown at Újfehértó, Eastern Hungary in the plantation of an assortment (gene bank) with 586 apple varieties. Each of the varieties were observed as for their dates of subsequent phenophases, the beginning of bloom, main bloom and the end of bloom over a period between 1984 and 2001 during this period the meteorological data-base keeps the following variables: daily means of temperature (°C), daily maximum temperature (°C), daily minimum temperature (°C), daily precipitation sums (mm), daily sums of sunny hours, daily means of the differences between the day-time and night-time temperatures (°C), average differences between temperatures of successive daily means (°C). Between the 90th and 147th day of the year over the 18 years of observation. The early blooming varieties start blooming at 10–21April. The varieties of intermediate bloom start at the interval 20 April to 3 May, whereas the late blooming group start at 2–10 May. Among the meteorological variables of the former autumnal and hibernal periods, the hibernal maxima were the most active factor infl uencing the start of bloom in the subsequent spring.
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The role of insurance in the damager mitigation of fruit orchards
117-122.Views:164Hungary faced many natural disasters in 2007. Due to the estimation of the Hungarian Chamber of Agriculture, the amount of natural disasters that occurred in Spring 2007 (frost, hailstorm and drought in April and May) is about 100 billion HUF. Frost and drought caused damage on about 250 thousand hectares of arable land. Currently, there are four insurance companies dealing with agricultural insurances in Hungary. Their income was nearly 20 billion HUF in 2003, whereas it barely exceeded 6.6 billion HUF in 2004. The reason behind the significant decrease of insurance fee is the state provision made in 2004 stopping fee subsidisation which originally started in 1996, enabling farmers to require a 25-30% reimbursement of the amount paid for insurance. Launching a state subsidisation again would greatly increase the number of agricultural insurances. The law about the national agricultural mitigation system passed last Autumn. This provision declares that the mitigation of agricultural damage that cannot be insured on a business-like basis is based on the common risk-taking by the state and the farmers. The introduction of this system is explained by the fact that the mitigation of damages through disaster can only be carried out if those affected also take part in it, according to EU rules.
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Scheduling of ornamental plant production
76-86.Views:130The production of ornamental plants represents an important branch of our horticulture. The growing area is relatively small (round 2800 ha), the production value, however, a rather large. Notwithstanding, its financial balance is inactive because the demand surpasses the supply copiously.
The most popular plants are as follows: carnation, gerbera, rose, bulbous flowers, chrysanthemum, other cutflowers, cutgreens and Gypsophyla — their total production value makes up to Ft 8-9 billion. The area of ornamental nurseries — about 800-900 ha — is to be found mostly on the western part of our country.
Our accession to the EU will have undoubtedly an impact on our ornamental plant production. We must take into account, that in greenhouse production the specialization extends all over the world, field production over a limited region. Our chances will not deteriorate by joining the EU. The buyer — chiefly because of ecological purposes — will prefer the domestic product to the foreign one. By the way, our products will be competitive, as far as quality or price is concerned, with those of western Europe. Last but not least, we may hope a greater saleability of home-bred, special varieties and cultivars, the so-called "hungaricums", both in the domestic as well as export markets. It is anticipated that we will have a good turnover with potted ornamental plants and flower seedlings. Our nursery products will become marketable too.
To exploit the opportunity, of course, the necessary conditions are to be created. First of all, we need development in research, with special regard to breeding, at the same time in education, in extension service, in the training of experts, on a high level. Some tasks can be solved, no doubt by improvement of the organisation within the branch. At the same time the state subsidy is indispensable in order to promote both the technical and the research activities. State subsidy is also necessary to build up more advantageous conditions of sales like in some foreign countries (e.g. the Netherlands).
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Genotypic difference of garlic (Allium sativum L.) cultivars for growth, bulb yield and yield related attributes at Tigray Province, Northern Ethiopia
46-52.Views:127The experiment was conducted at Ofla district from June to October of 2017 and 2018 cropping season to select high yielding and adaptable garlic cultivar(s). Six garlic cultivars namely Chefe, Tsedey, Holeta local, Kuriftu and Bora-4 and one Ofla Local were evaluated. The experiment was arranged in completely randomized block design (RCBD) with three replications. Different growth, phenology, yield and yield related data were collected. Leaf length, leaf width, bulb diameter and length were significantly (p<0.05) influenced by garlic cultivars in both cropping seasons. Bulb weight and number of cloves per bulb were highly significantly (P<0.01) influenced by cultivars in both cropping seasons. Also, marketable yield, unmarketable yield and total yield ha-1 were highly significantly (P<0.01) influenced by cultivars in both seasons. During 2017 and 2018, the highest marketable bulb yield was observed from Ofla Local cultivar with an average value of (8.86 t/ha) and (9.06 t/ha) respectively. During the 2017 cropping year, the maximum and significantly higher total bulb yield of 10.21 t/ha was recorded on the Ofla Local cultivar. Moreover, in 2018, this cultivar provided the maximum total bulb yield of 9.80 t/ha. Therefore, the Ofla Local cultivar showed the greatest performance for growth, yield and yield related attributes. Thus, it is recommended for cultivation in areas having similar agro-ecology. To improve the productivity of garlic, it is important to study and identify the optimum fertilizer level and spacing in the study area.
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Estimating of water consumption of cherry trees
15-17.Views:190Despite of its importance there is no exact information on water use of new scion/rootstock composite trees, which would be needed to optimized irrigation. Our research purpose is to define exact water-demand of different rootstock/scion composite trees, calculating seasonal weather changes and by using the results decrease irrigation costs. The investigations are carried out in Soroksár, at the Experimental Farm of Corvinus University of Budapest in May 2008. From among the investigated trees two are budded onto Prunus mahuleb `Érdi V' seedlings, two on "Korponay' seedlings. The sapflow measurements are carried out using Dynamax Flow 32 equipment with Dynagage trunk sensors. The first daily maximum of sapflow was around 10:00 a.m. (2.5 kghour I), the second maximum was always between 14:00-15:00 p.m. (2 kgday- I). Comparing to the very intensive morning water uptake by 20:00 p.m. the water flow slowly reached the minimal level. Significant differences can be seen on rootstocks: trees on `Korponay' rootstock always showed more intensive sapflow and a higher morning peak than trees on `Árdi V'. But later during the day they have the same run.Based on our results the water quantity transpired only by the trees reached in May 86-104 mm, while the precipitation was only 42.4 mm. This means a 40-60 mm deficit in the orchard, which should have been supplied by irrigation despite of the satisfying horticultural performance of the orchard. In the first half of the month beside the steady vapor pressure deficit the shoot and leaf surface growth could cause the increased sapflow.
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New sour cherry cultivars selected from local sources
79-80.Views:286New sour cherry cultivars selected from local sources
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High antioxidant - and anthocyanin contents of sour cherry cultivars may benefit the human health: international and Hungarian achievements on phytochemicals
45-47.Views:407Evidence suggests that a diet with high fruit and vegetable consumption may decrease the risk of chronic diseases, such as cardiovascular diseases and cancer, and phytochemicals including phenolics, flavonoids and carotenoids from fruits and vegetables may play a key role in reducing chronic disease risk. Recent research has proved that sour cherry (Prunus cerasus L.) is a valuable natural source of some bioactive compounds important in human health preservation. According to the published data, the most important biological effects of sour cherry are connected — directly or indirectly — to their endogenous antioxidant behaviour as well as to their specific pattern of anthocyanin components. In the present work, we measured the total antioxidant capacity of some Hungarian sour cherry varieties in combination with their anthocyanin-, and vitamin-C content. In 2003, twelve clones were selected and grafted from a local sour cherry population called "Bosnyák" sour cherry grown in small home gardens and farms of the village Csengod (Great-Plain Region, South Hungary). Other Hungarian sour cherry varieties, i.e. cv. Újfehértói fürtos, cv. Érdi bőterrnő, cv. Debreceni bőterrnő, cv. Csengődi and cv. Kántorjánosi served as a control.
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Changes in Some Quality Criteria During Controlled Atmosphere (CA) Storage of Peaches
58-61.Views:120Intensive research has been implemented on different aspects of the bee pollination of pear in Hungary in the past decade, extending to the following topics: the effect of the opening sequence of the flowers within the inflorescence of pear cultivars on the effectiveness of bee pollination; the nectar production of flowers of pear cultivars; intensity of honeybee visitation and their foraging behaviour at the flowers of pear cultivars; flower constancy of honeybees to pear plantations; competition by weeds in bloom for bee pollination in flowering pear orchards; and some aspects to the bee pollination strategies of pear plantations (the effect of the limitation of bee pollination period on the set and the yield, possible compensation of frost damage of flowers by bee pollination in pear orchards, the optimal size of single cultivar block in pear plantations). The results of these studies are reviewed in this paper and some important aspects that need further studies are outlined.
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Nutritional quality, fruit shape and relationships among exotic and local Capsicum pepper genotypes in Uganda
33-39.Views:336Twenty-one hot pepper genotypes comprising of local (15) and exotic (6) types (C. annuum, C. frutescens and C. chinense) were characterized for selected fruit traits after propagation in a glasshouse at the Makerere University Agricultural Research Institute Kabanyolo in Central Uganda using a completely randomized design with three replicates. Ripe fruits were harvested and analyzed; traits evaluated were all significantly different at P<0.05 with variations in quality attributes. The genotype OHA-B305-10 had the highest ascorbic acid content (128.86 mg/100 g) and is recommended for improvement of both local and exotic genotypes targeting the fresh market. Genotypes CAP0408-12 and UG2 WE0511-22, with highest total soluble solids (16.17 ºBrix) and dry matter content (28.59%), respectively should be used in improvements for industrial use or processing to products such as chilli powder or flakes. BRS-M205-04 with highest titratable acidity (1.04%) can be used in enhancing shelf life of genotypes with low titratable acids as well as for the fresh market. In spite of the intraspecific relationships among genotypes, significant differences were observed in their quantitative traits. These genotypes will, therefore, be useful in improving the quality of hot pepper fruit in Uganda.
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Virus and virus-like diseases of grapevine in Hungary
25-36.Views:262Viruses and viroids are submicroscopic infectious particles which can cause disease symptoms on grapevine. These parasites are depending completely on the energy metabolism of the plant cell. To enter the host cell plant viruses depend on injuries or on transmission via invertebrates (insects, nematodes, etc.). Viruses are classified by many characters including particle morphology, host range and information content of the genome. At present about 70 viruses including 7 viroids infecting grapevine are known. In single or mixed infections they are potentially detrimental to the quality and quantity of grape production in any growing area of the world. Some viruses can cause severe economic damage in vineyards. In Hungary many important viruses and viroids have been detected in grape. This review summarises characteristics of viruses and the results of detection and characterization of virus and virus like diseases of grapevine in Hungary. The identification of the causal agent, its transmission, geographical distribution and the development of the diagnostic methods are also discussed.
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In vitro plant regeneration from immature embryo axis and cotyledons of common bean (Phaseolus vulgaris L.)
93-97.Views:129Phaseolus vulgaris L. is the most important economic species within the genus Phaseolus. It is grown in all parts of the world. Genetic improvement by conventional breeding has met considerable success, although production of hybrids between species within the genus has been limited due to sexual incompatibility. Recent advances in tissue culture have offered the opportunity to produce cultivars, which could not be obtained by conventional breeding methods. The use of tissue culture and genetic engineering is viewed as a logical approach to improve bean production. Gene transfer techniques will have a great impact on legumes. Although the concept of cell totipotency is widely proved, in vitro morphogenesis has not yet been achieved for a large number of cultivated beans. Regeneration protocols are strongly influenced by the genotype. In tissue and cell culture of beans, the factors controlling shoot morphogenesis and somatic embryogenesis are still unknown. The reported data suggest a possible way for future research.
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Floral biological studies on pear cultivars in relation to fire blight susceptibility
25-30.Views:166Floral biological characteristics that may influence cultivar susceptibility to fire blight were studied in 10 pear cultivars in two Hungarian orchards from 1999 to 2003. The receptaculo-ovarial, automorphic nectary is usually bigger in tolerant cultivars than in susceptible ones. Nectary stomata are meso- or xeromorphic. Susceptible cultivars tend to have more xeromorphic stomata, where guard cells are located 1-3 cell rows below the epidermis. The size of nectar chambers is usually smaller in susceptible cultivars. Floral nectar, consisting mainly of glucose and fructose, is more abundant and less concentrated if the cultivar is susceptible to fire blight. The amount of chlorogenic acid was higher in the flowers of tolerant cultivars than in susceptible ones.
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Recent developments in biochemical characterization of Vitis vinifera L. varieties in Hungary
57-61.Views:167Isoelectric focusing is an effective and well reproducible method to provide information for identification of various plant species and clones if breeding or other genetic modification(s) for a given species are reflected in changes of an isozyme pattern. The method has been used for characterization of plant proteins and enzymes and for identification of various species and varieties. Our aim was to continue our several-year-work carried out on a wide variety of grapevine varieties and to reveal whether analyses of esterase and peroxidase isozyme patterns are suitable to distinguish various grapevine varieties. Therefore we compared esterase and peroxidase isozyme patterns of various species. Plant samples were obtained from Szigetcsep, Kecskemet, Tokaj and Eger. The following samples were analyzed: Pinot gris, noir, blanc, Chardonnay, Riesling Theses, Chasselas from Szigetcs6p, Bianca and his parents Eger2 and Bouvier from Kecskemet, Furmint and Hárslevelű from Tokaj, Kékfrankos and Zweigelt from Eger. To identify various species according to their esterase isozyme patterns the after blooming phenological phase while according to their peroxidase isozyme pattern the dormant phenological phase was found as optimal sampling time.
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Sugar composition of floral nectar in sour cherry cultivars
109-113.Views:185The composition of floral nectar in sour cherry cultivars studied in 1997 at Újfehértó was in agreement with our previous data, the three most frequent sugar components being glucose, fructose and sucrose. Nectar secreted at night is generally more diluted than nectar produced during the day. None of the nectar samples produced at night reached the threshold value (100 mg/ml) of bee visitation. In the majority of cultivars the difference in concentration between night and day nectar is not too high, but in two cultivars, 'Korai pipacs' and ‘Újfehértói fürtös’ the difference is quite significant. Most sucrose was found in the nectar of cvs. 'Érdi jubileum' and ‘Újfehértói fürtös’, but a high amount of sucrose was measured also in the flowers of 'Korai pipacs' and 'Meteor USA'. Nectar concentration varies from opening of the flower to petal fall to a smaller or greater degree, depending on the given cultivar. From the 9 sour cherry cultivars studied ‘Újfehértói fürtös’ and Tandy 48' had the most attractive nectar for bees. There was no correlation between nectar composition and free fertilisation. On the basis of nectar composition the majority of the sour cherry cultivars studied can be classified into the sucrose-rich category; only one cultivar, 'Érdi jubileum' had a sucrose-dominant secretory product. The composition of nectar in the studied sour cherry cultivars is preferred by bees.
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Agrobacterium transformation of Rhodiola sp.: current status and limitations
37-42.Views:3The study of secondary metabolites has led to the discovery of new drugs for treating human diseases. However, consistent plant supply can be challenging, leading to the use of plant tissue culture techniques such as hairy root culture. Hairy roots have stable genetics, lateral branching, and can produce secondary metabolites, including alkaloids, flavonoids, and terpenoids. Research on hairy roots as a subject began in the late 19th century, and for the last four decades, hairy roots have been utilized for producing secondary metabolites and recombinant proteins. This article focuses on Rhodiola species - genus of perennial plants that belongs to the family Crassulaceae - and its potential as a source of secondary metabolites using hairy root culture techniques. Rhodiola sp. is widely distributed throughout the Arctic regions of the Northern Hemisphere, with several species having significant medicinal properties. The article discusses the possible use of hairy root cultures for the production of Rhodiola secondary metabolites, including salidroside and rosavins, which have demonstrated significant pharmacological activity in various studies. The use of elicitation and genetic engineering techniques to boost secondary metabolite production in Rhodiola hairy roots is also explored. Overall, the article highlights the potential of Rhodiola hairy root cultures as a valuable source of secondary metabolites with medicinal properties. However, despite some studies Rhodiola hairy root induction and culturing still remains highly unexplored.
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Precision geoinformatical system of the pear gene-collection orchard
43-50.Views:217The principle task of the sustainable development is the preservation of the genetic variety, which is similar challenge in the horticulture regarding the sublimation of fruit species. The breeders of the traditional fruit strains give stock to the sustenance diversity of the agro-environment on the species and landscape level. In 2009, hyperspectral images have been taken by AISA Dual sensors from the pear gene pool in Újfehértó, Hungary. The hyperspectral data cube (in the wavelength range of 400-2500 nm, with 1.5 m ground resolution) ensured possibility to make the spectral library of pear species. In the course of the simultaneously field work the spatial position and individual extent of all pear trees was defined to set up a detailed GIS data base. The water stress sensitivity of single species and the descriptive spectral curves were determined with common evaluation of the spectral and spatial data. Based on the unique methodology processing and the hyperspectral data base suitable strains can be chosen for agro-environment and let take adaptive stocks regarding climate change into the genetic grafting work. Furthermore we could determine and map the sparsely species in the region with the help of the hyperspectral data.
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Bioactive phenols in leaves of Forsythia species
57-59.Views:222Nowadays a number of lignans (arctigenin, matairesinol, pinoresinol and phillygenin) have come to the fore in research due to their various biological activities. In this paper the accumulation of these constituents in leaf extracts of Forsythia plants (F. intermedia, F. ovata 'Robusta’ and `Tetragold', F. suspensa, F. viridissima) was quantified using a new isolation method, supercritical CO2 fluid extraction. The total phenolic and flavonoid contents, the antioxidant capacity and the aglycone lignan profile were determined in leaf extracts of Forsythia species. Within the phenols, the flavonoids were only present in small quantities, but the amount of aglycone lignans was extremely high. F. ovata `Robusta' had the highest total lignan content (103.8 mg/g) of all the Forsythia species. The main lignan in this species is arctigenin, which normally makes up about 60% of the total lignan content, but in the case of F. ovata `Robusta' this value was 96.1%. Since this arctigenin content is outstanding compared to that of other Forsythia species, it could be promising to develop a fermentation technology for the production of this natural compound.
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Long term investigations of flowers and leaves on mainly non-domestica plums
73-79.Views:175The author dealt with plum species representing different eco-geographic areas by their genetic adaptation and their hybrids, as European (P. domestica, P. italica, P. cerasifera), Asian (P. salicina, P. simonii, P. ussuriensis), American (P. americana, P. besseyi, P. munsoniana, P. tomentosa). The rootstocks of the trees examined were seedlings of C. 679 myrobalan with the exception of Laroda and Santa Rosa II, which were grown on three different stocks: seedlings of C. 174 myrobalan, C. 449 bitter almond and C. 471 sweet almond. The size of peduncle, length of pistil, stamen number per flower, relative stamen number (SN/PL) have been suitable for description and distinction of varieties. Similarly shape of leaves, length of petiole, length and width of blade helped the identification.
The ratio of the dimensions of leaves, length of petiole and of leaf blade, also contributed to the distinction of European, Asian and American plum species, notwithstanding their relations with ecological conditions as well as historical, technical properties, pomological features, etc. Computed indicators (relative stamen number and shape-index of leaves) also have been useful data.
Significant correlations have been found between colour of nectaries and mean values of variety-groups. The potential values of non-European varieties for purposes of commercial production could be forecasted from the point of view of quality, ecological, pomological as well as market value. It is important, however, to know the effect of the rootstock and growing site as well as their interaction, on the one hand, whereas the resistance or tolerance of the varieties as limiting factors, at least to the sharka (Plum pox) virus, Xanthomonas pruni, on the other hand (cf. Surányi & Erdős, 2004a and 2004b).
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Introduction of alkanet (Alkanna tinctoria (L.) Tausch), a traditional dye plant into cultivation
41-46.Views:302As a part of the research project to establish natural sources of plant pigments, possibilities of introduction of Alkanna tinctoria (alkanet) into cultivation were studied.
As a result of the germination experiment, the relevance of 21 days' duration of germination procedure was proved. To get high germination rate alkanet fruits are proposed to pretreat by gibberellinic acid (GA3) in the concentration of 400 ppm, overnight before sowing. This method results approximately 50 % germination rate.
The morphological and production properties of alkanet roots are characterized during ontogenesis. Transplanted populations can be characterized by numerous, thick and heavy roots comparing to the spontaneous ones. Thus, seed sowing and transplantation proved to be an effective method for cultivation of the species.
According to our results it can be concluded that in cultivation the optimal harvesting time of roots is at the end of the second vegetation cycle, when the dry root mass of the individuals is about 10-20 g with 3,0-3,5 % accumulation level of active substances.
Considerable seasonal variability have been found influencing not only the root masses, but also the accumulation levels of alkannin derivatives. In a more humid vegetation cycle the root size and mass as well as the content of active substances are much higher.
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Effects of methyl jasmonate, salicylic acid and phenylalanine on aloe emodin and aloin in diploid and tetraploid Aloe barbadensis
Views:221Aloe vera is one of the most famous medicinal plants. Aloin and aloe emodin are the most important active compounds in this plant. The purpose of this research was the comparison of aloin and aloe emodin production after the elicitation by methyl jasmonate, salicylic acid, and phenylalanine in diploid and tetraploid Aloe vera plants in greenhouse conditions. The plants were treated with the concentrations of 25, 50, and 100 µM. The amounts of aloin and aloe emodin were determined 24 and 48 hours after application of the treatment. HPLC analysis showed that the leaves of the control diploid plants (without applying elicitors) had more aloin (1.20 fold) and aloe emodin (1.14 fold) than the control tetraploid plants. The maximum concentration of aloin (1.15 ± 0.07 µg mg-1 dry weight) was obtained after the elicitation by 25 µM methyl jasmonate, 24 hours after treatment, in diploid plants) 6.36 fold compared to the control (0.18 µg mg-1 dry weight (. In addition, the maximum concentration of aloe emodin (0.28 µg mg-1 dry weight) was obtained after the elicitation by 25 µM salicylic acid, 24 hours after treatment, in diploid plants) 6.18 fold compared to the control (0.04 µg mg-1 dry weight)). The long-term effect of three studied elicitors (after 240 days) on plant health and survival was also studied. This investigation showed that only methyl jasmonate at a concentration of 100 µM was resulted in the death of Aloe vera plants.
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Apple powdery mildew caused by Podosphaera leucotricha: some aspects of biology
19-23.Views:363Apple powdery mildew (Podoshphaera leucorticha) occurs wherever apples are grown. One of the most important fungal disease of apple which causing severe econimic loss on susceptible apple cultivars. Biology of the pathogen is widely investigated all over the world in the past 100 years. In this review, a summary from this enormous research is made for biology of apple powdery mildew in the following aspects: geographical distribution, morphology, taxonomy of the causal agent, symptoms, host susceptibility, resistance durability and disease cycle.