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  • Prolonging the vase life of cut Carnation 'GIOKO' by using different chemicals
    65-68.
    Views:
    175

    Cut flowers of Dianthus caryophyllus L. cv. GIOKO were treated with different concentrations of sucrose and in combination with 1­methylcyclopropene (1-MCP) to compare the effect of these treatments with floral preservative (`Spring') on the longevity of flowers. Distilled water was used for preparing all solutions. The control flowers were held in distilled water. Clorox at 2 mL- I was added to all treatments containing sucrose and it was also applied as a separate treatment. The vase life of cut carnations was significantly prolonged due to the use of chemical treatments, as compared to the untreated control. The longest vase life (18.33 days) was obtained by using 1-MCP 0.5 g m-3 for 6 h treatment. All concentrations of sucrose had a positive effect on flower diameter. The best treatment in this respect was 1 -MCP with 30 gL-I sucrose. 1-MCP treatment significantly increased the chlorophyll content, as compared to the control or the "Spring" treatment. The highest values in this respect were obtained by 1 -MCP treatment alone or with the lowest level of sucrose. The effect of these treatments on the pH of solutions is discussed.

  • The hydroculture of calla
    90-92.
    Views:
    95

    Hydroculture was established in the early 40's. This technology became wide-spread in 60's. Because of economic considerations it played little role in Hungarian ornamental plant growing. The forthcoming joining of the EEC as well as the strict enviroment protection regulations, this technology is likely to spread in our country and like in most of the West European countries, cut flowers will be grown in hydroculture. Closed systems match the most strict environmental regulations. Calla can be well adopted to this technology because of its origin and water demand.

    We have studied three growing methods: PUR-agrofoam, container and soil-heated, of which soil-heated proved to be the best significantly.

  • Evaluating vase life and tissue structure of some compositae (Asteraceae) species
    87-89.
    Views:
    168

    The vase life of cut flowers and effects of various chemicals was examined with the help of a pulse treatment. According to the results using of chemicals (preservatives, disinfectants as well as blocking of synthesis of ethylene) is ineffective if it is used after seeding This shows the great importance of harvesting time.

    Using 8-HQS or l-MCP + 8-HQS proved to be the best for vase life in most of the samples. Using these materials did not prevent the appearance of air bubbles in the stems and absorption could be observed continuously.

    To examine the tissue structure reaction of chemicals stems were stained with toluidin-blue, and high of absorption was measured. It was found that in cases, when absorption was bad, small air bubbles blocked the xylem vessels.

    All the species examined (Aster linosyris, Achillea collina, Aster novi-belgii, Inula britannica, Solidago canadensis, Inula ensifolia, Senecio jacobea) show similar reactions to chemicals because they are the members of the same family.

  • Post-effects of cytokinins and auxin levels of proliferation media on rooting ability of in vitro apple shoots (Malus domestica Borkh.) 'Red Fuji'
    26-29.
    Views:
    207

    Rooting ability of in vitro apple shoots of 'Red Fuji' grown on proliferation media with different hormone content were tested at three IBA levels in root induction media. Rooting percentage could be slightly increased with an increase in IBA concentration in proliferation media. The highest IBA concentration (3.0 mg 1-1) in root induction media showed strong inhibitory effect on rooting capacity of in vitro shoots. The highest rooting percentage (95%) could be achieved by shoots grown on proliferation media containing TOP or BA+KIN as cytokinins before rooting.

     

  • Examination of valuable ingredients of some wild fruits
    71-74.
    Views:
    153

    A possible way of the development of Hungarian agriculture is the selection and growing of new fruit species and varieties featuring special qualities, with high biological nutritive and health protecting properties due to their natural composition. A reserve for such new fruits is the native dendroflora, e.g. those wild-growing trees and shrubs of Hungary, which bear edible fruits. The publication is giving a summary of chemical analyses done on the fruits of the plants listed below. The research team on the project started the work in 2001 with

    woody species (genera) as follows:

    • common elder (Sambucus nigra) clone named Szcs-1, Szcs-2, Szcs-3, Szcs-4, Szcs-5, SzcsK-1, SzcsK-2
    • dog rose (Rosa canina) types: clones named Sz-1, Sz-2, Sz-3, Sz-4 and Sz-5 .
    • native rowans: Sorbus dacica, S.rotundifolia, S. degenu, S.bakonyensis cv. Fánivölgy
    • hawthorns: Crataegus monogyna, C. orientalis,C..v lavallei.
    • cornel cherry: Cornus mas cv. Császló.

    The present paper is reporting on the content of the following compounds in the fruits: dry matter (refractometric values), total acid content, ascorbic acid, 13-carotene, pectin, minerals and carbohydrates. The results have shown that these wild fruits have excellent composition. Besides their curative effects, their content of minerals, ascorbic acid and 13-carotene has surpassed that of the traditional fruits. These fruits are rich in ascorbic acid, 13-carotene and pectin. The high content of the above-listed, biologically active compounds makes the new wild fruits studied suitable for the preparation (and later: mass-production) of special curative and exclusive products.

  • The effect of different cytokinins on chlorophyll content and morphological features of in vitro Nidularium ’Kertész Jubileum’
    47-51.
    Views:
    229

    During in vitro multiplication of Nidularium ‘Kertész Jubileum’, 20 g/l sucrose, 5 g/l agar, 100 mg/l inositol, and different concentrations of benzyladenine (BA), benzyladenine-riboside (BAR), kinetin (KIN), meta-topolin (mT) were added to the MKC (Knudson, 1946) basal medium. Furthermore, 0.1 mg/l naphthaleneacetic acid was used to every medium. Number of shoots, length of leaves, number and length of roots, chlorophyll (a+b) content were examined and evaluated with Ropstat statistical software. As compared to the other cytokinin, significantly most shoots were obtained in the case of applying BA. Increasing of BA-concentration (as far as 2 mg/l) enhanced shoot number (from 10.92 to 19.26) but 4 mg/l BA resulted only 6.63 shoot. The less efficient cytokinin was KIN, in most cases no more than about 2 shoot was achieved. Regarding the length of leaves, the higher level of BA effected averagely the shorter leaves (from 24,46 to 7.31 mm). KIN effected significantly the longest leaves (43.4-61.29) in inverse proportion to the concentration. The same cytokinin resulted the most (and the longest) roots with the highest rooting percentages, but more KIN decreased the number and length of roots (from 7.95 to 4.4 and from 38.49 to 22.73 mm). There were no definite correlation between cytokinin concentration and chlorophyll (a+b) content, but the highest doses resulted decreasing (except of meta-topolin which leads to the lowest values). Summarizing, BAR effected the highest contents (mostly more than 1400 μg/g), particularly in the case of 1 mg/l (1807.3 μg/g).

  • Effects of activated charcoal on rooting of in vitro apple (Malus domestics Borkh.) shoots
    98-101.
    Views:
    134

    Rooting of in vitro 'Royal Gala' shoots was studied under different conditions of root induction and root elongation phase. The rooting capacity was affected by both rooting phases. Very high rooting percentage could be reached with both liquid and solid root induction media. Raising the temperature from 22 °C to 26 °C during root induction phase increased the rooting percentage. Presence of activated charcoal in root elongation media can affect the number of roots per rooted shoots and can increase the rooting percentage, the length of roots and the rate of survival depending also on other conditions during rooting. Presence of NAA in root elongation media reduced the number and the length of roots considerably. Favourable effect of activated charcoal on rooting was mainly due to adsorption of NAA.

  • Effects of indole-3-butyric acid levels and activated charcoal on rooting of in vitro shoots of apple rootstocks
    25-28.
    Views:
    249

    Rooting responses of rootstocks cvs. JTE-F1, M. 26 and MM. 106 were studied to different concentration of IBA in root induction media and to presence of activated charcoal in root elongation media. High rooting rate (>90%) could be achieved in cvs. JTE-H and M. 26, while cv. MM. 106 showed weak rooting ability at each IBA level tested. Increasing IBA content depressed the rooting only in cv. M. 26. Presence of activated charcoal decreased considerable the rooting rate in cv. M. 26 and decreased the number of roots in cvs. JTE-H and M. 26. These cultivars developed longer roots on media containing activated charcoal, while cv. MM. 106 did not showed any reaction for it.

  • Colour stability of the flowers in some modern rose varieties in Hungary
    61-66.
    Views:
    149

    A variety trial has been carried out to study the colour changing — colour stability of the flowers of some modern roses, especially of Hungarian varieties. 100 floribundas and polyanthas were observed. Colours of the petal surface were examined at three fenological stages of the flowers: at the bud, at the young open flowers and at the aged open flower stages. The colour difference was described in HLS standard. Our work shows that valuable Hungarian varieties can be found in both studied classes, but more floribundas bred in Hungary had good colour stability than polyanthas. The best Hungarian floribundas were 'Pest' and 'Remenyik Sándor emléke', although 'Regen', `Szent Lász1ó em­léke' and 'Szabó Dezső emléke' were quite good. The best Western-European floribunda rose was 'Perneille Poulsen'. Their colour stability was reliable in different situations. Colour changing of the two best varieties was almost unperceivable from the bud to the young petals and from the young to the aged petals. In the polyantha class there was only one perfect variety: the Czech `Cs1 Cerveny Kriz'. The 'Fairy Damsel' was almost excellent as well. The best Hungarian polyanthas were `Domokos Pál Péter emléke' and 'Savaria'.

  • Ornamental plants in Hungary Part I. Protected cultivation
    102-105.
    Views:
    170

    Between 1950-1989, the production and trade of ornamental plants in Hungary was characterised by meeting the demands of the home and that of the Eastern-Block market and by a minimal external trade with the Western countries. After the socio-economical changes in 1989/90, the trade of flowers gradually became liberalised and the Hungarian growers had to face the concurrency of steadily increasing import from the West and from all over the World. This tendency coincided with the physical and mental degradation (outdating) of most of the glasshouses, along with the decline of several former large growers of the communist type (state or cooperative), the appearance and growth of new private companies and the building (rebuilding) of new plastic houses and (mainly second-hand) glasshouses. In spite of the above-listed problems, the production as a whole did not (or only slightly) decrease and/or even an increase occured in many areas mainly in the open-ground production. In 1998, the protected flower cultivation comprised round 110 ha of glasshouses, 180-220 ha of plastic structures and 3-5 ha of frames, with the main crops as follows: cut flowers and cut foliage 220-240 ha; pot plants (with geraniums) 30-40 ha; bedding plants (without geraniums) 20-25 ha; "transit--greenhouses (for redistribution only) 3-5 ha: other (eg. propagation of woody ornamentals) 3-5 ha. The structure of open-ground production was as follows: Total 1150-1210 ha, including: Nursery products: woody ornamentals 880 ha; perennials 10-15 ha; rose bushes 30-35 ha. Other open-ground crops: flower bulbs 50-60 ha; dried flowers 130-140 ha; open-ground cut flowers 25-30 ha; flower seed 30-35 ha; (biennial) bedding plants 10-15 ha.

  • The effect of Regalis® (prohexadione calcium) on the reduction of fire blight (Erwinia amylovora) severity in apple trees
    61-66.
    Views:
    162

    Due to the lack of effective and non-phytotoxic materials for control of the blossom and shoot blight phase of fire blight in pome fruit trees, two novel control strategies have emerged: shoot growth retardation by bioregulators and applying resistance inducer compounds. Prohexadione calcium (ProCa) is the active ingredient of the bioregulator Regalis® registered in several European countries. The reduction of shoot growth elongation is the most obvious effect of ProCa. Furthermore, it causes significant changes in the spectrum of flavonoids and their phenolic precursors, leading to the considerable reduction of susceptibility to fire blight.

    In Poland, potted one-year-old apple trees of cvs. Gala Must grafted on M.26 and Sampion on M.9 (in 2001) as well as Gala Must on P.60 (in 2002) were treated with Regalis® at a range of concentration of 250, 150 or 150 + 100 ppm, respectively. The inoculation of shoots was made with the strain No.691 of E. amylovora (107 cfu/ml), on the 7th and 21st'day after treatments with Regalis. In Hungary, during the years of 2002 and 2003 one-year-old container grown apple trees of the cvs. Idared/M.9 and Freedom/M.9 were treated with the prohexadione-Ca, the active ingredient of Regalis® 100, 150 or 200 ppm, two weeks before inoculation with the Ea 1 strain of E. amylovora (107 cfu/m1).

    In Poland, the suppression of fire blight in shoots reached up to 80%, depending on concentration and application time of Regalis®. In Hungary, the effect of prohexadione-Ca treatments, determined by the length of necrotic lesion developed, proved to be better than that of streptomycin used for comparison.

  • Results in the determination of some Hosta varieties by the method of isoelectric focusing
    90-92.
    Views:
    112

    Mass propagation of 5 newly introduced Hosta varieties was carried out by the method of micropropagation. Our aim was to determine exact variety specificity after the micropropagation period in the pattern of peroxidase isoenzymes by isoelectric focusing in pH 3-9 range and to determine that phenological phase of mother plant in which the isoenzyme pattern of mother plant can safely be comparable to the isoenzyme pattern of micropropagated descendants. The isoenzyme patterns of descendants were similar to the mother plants of the same hybrid lines. The older leaves seemed to be not so suitable for examination than newly developed ones despite of the higher activity of peroxidase enzymes. There were big differences in isoenzyme patterns of leaves in different phenological phases. With this quick and easy method Hosta varieties could be selected already in the very early stage of micropropagation.

     

  • Morphological, physiological features and differences of Vriesea splendens ’Fire’ plants during in vitro multiplication and rooting
    Views:
    114

    During in vitro multiplication and rooting of Vriesea splendens ’Fire’, 0.1, 0.2, 0.4 and 0.8 mg l-1 benzyladenine (BAP), benzyladenine-riboside (BAPR), kinetin (KIN), meta-topoline (MT), indole-butyric acid (IBA) and naphthalene-acetic acid (NAA) were added to basal Murashige and Skoog (1962) MS medium. As compared to the hormone-free control, plants developed significantly more shoots on medium supplemented with almost all cytokinins (excepting KIN), especially BAP resulted the highest multiplication up to almost 26 shoots. Enhancement of cytokinin concentrations increased shoot number (and in case of BAP, peroxidase activity) but decreased plant height and rooting parameters. Regarding root production, both auxins were definitely beneficial (0.2 mg l-1 NAA resulted more than 7.5 roots and higher auxin concentrations efficiently stimulate root elongation); however, KIN had similar effects. After a three-month duration time of acclimatization, we observed that plants which were previously cultured on medium containing certain cytokinins (KIN in all doses and 0.1 mg l-1 MT) or both auxins had greater survival, moreover, as negative after-effect, higher cytokinin concentrations reduced the number of survived specimens.

  • Effect of sowing dates and NPK levels on active substances in seeds of isabgol plants (Plantago ovata Forsk, L.).
    89-92.
    Views:
    126

    Two field experiments were carried out in the Experimental Station and Laboratory of the Vegetables and Floriculture Dept., Fac. of Agric., Mansoura Univ., during the winter seasons of 1995/96 and 1996/97. The investigation aimed to study the effect of different sowing dates and NPK fertilization levels, as well as their interactions on active substances extracted from Isabgol plant (mucilage contents). Four sowing dates and six NPK levels were studied. The main findings in this investigation can be summarized as follows:

    1. Plants grown in the first sowing data (Nov. 1st week) produced the highest content of mucilage as g/5 g seeds or as percentage of mucilage per plant.
    2. The addition of NPK fertilization significantly increased the mucilage content of seed and its percentage per plant. The highest content and percentage were in plants treated with the fourth level of NPK (178.0 g/3m2).
    3. Concerning effect of interaction between sowing dates and NPK levels on mucilage content, the highest content and percentage per plant were produced in plants grown in the first sowing date and treated with the fourth level of NPK.
  • Biotechnologies for ornamental plants: some insights to the Brazilian productive chain
    51-59.
    Views:
    251

    The world industry of ornamental plants is under wide transformations regarding the changes in the cultivation, management, post­harvest technologies and the use of new (bio)technologies to assist the improvement and development of new varieties. This industry worth more than US$ 20 billion, but Brazil shares only 1% of this value. To compete at this market with permanent technological innovation, the country needs to be tuned in with the progresses in the biotechnologies applied to the productive chain of ornamental plants. In this revision, we analyze the Brazilian and world ornamental market emphasizing the role of the biotechnologies in the modernization and increase of the competitiveness of the sector.

  • Exotic woody plants inclining to escape in the Buda Arboretum under strong urban effect in Budapest
    93-97.
    Views:
    129

    The Buda Arboretum of the Szent István University is situated in the western-middle part of the city of Budapest. It contains 1640 taxa of woody plants on an area of 7.5 ha. The sheltered position and the urban micro- and meso-climate is favourable for warm-loving, even subtropical plants, of which many species not simply stay alive, but flower, bear fruits and are spreading by seedlings in suitable spots. These instances call our attention to the possibilities that urban climate offers for landscapers, but also to over 110 species, which can be garden escapes in the future. Full list of plants, which tend to escape in the Arboretum is given in the paper.

     

  • Influence of different growth regulators on the in vitro morphogenesis of an ornamental variety of carnation
    55-57.
    Views:
    137

    Callus formation, as a prerequisite for the induction of somaclonal variability, was achieved successfully with certain molar ratios between 2,4-dichlorophenoxyacetic acid and benzyladenine. Regeneration of new plants from shoot apex meristems could be significantly improved by the combined addition of very low amounts of indolebutiric acid, benzyladenine and gibberelic acid, dissolved in the Murashige-Skoog nutrient medium. These in vitro treatments may contribute to a more efficient micropropagation of the Rimini variety of carnation.

  • Model experiments for establishment of in vitro culture by micrografting in apple
    47-49.
    Views:
    190

    Micrografting was used in our experiments for establishment of in vitro culture from one rootstock (`JTE-F') and three scion cultivars (`Remo', 'Rewena' and `Reanda') of apple. Shoot tips of these cultivars were harvested from field and grafted onto in vitro rootstock cultivars. Their survival and development were studied. 42-93% of shoot tips survived and developed further depending on cultivar. Impermanent browning of sticking agar-agar could be observed in 21-25% of the micrografts depending on cultivars but discolouration of agar-agar ceased within one week and did not cause any death of shoot tips. We used micrografting successfully for establishment of in vitro culture from cultivars, from which earlier with conventional methods the culture establishment was not possible because of hard tissue browning. However, further studies are necessary to ensure the survival and development of shoots after removing them from micrografts.

  • The role of rejuvenated and adult forms of English oak (Quercus robur) in in vitro cultures
    81-83.
    Views:
    142

    In vitro plant material of clones (Q. robur) NL 100 A (adult) and NL 100 R (rejuvenated) received from Germany (A. Meier-Dinkel, 1995) were used in these experiments. WPM medium was used for the multiplication phase. Plantlets were subcultured monthly. Differences in quality and colour of the adult and rejuvenated cultures induced us to follow and compare the changes of mineral- and chlorophyll content and dry weight during the propagation phase. Mineral and chlorophyll content as well as dry weight were measured weekly on three samples during the subculture period.

    In the case of propagation rates we stated, they were similar around the year, but both clones had a high peak in April. Examining the cation-content, we detected that, the plantlets had a highest quantity of several elements during the 2nd and 3rd week of subculture. The iron content was the highest in the 1st week and after that it decreased continuously. It is supposed, that the content of iron is not enough in the media. The chlorophyll content of the rejuvenated clone was higher than that of the adult one.

    In the rooting experiments it was stated that, after one-week cold treatment the rooting ability was the best.

     

  • New in vitro micrografting method for apple by sticking
    79-83.
    Views:
    255

    The requirements for in vitro micrografting in apple are described. In vitro multiplicated shoots of cv. Royal Gala were the sources of rootstocks and scions after different pre-treatment, respectively. Oxidative browning of cut surfaces could be inhibited by the use of antioxidant mixture during grafting process. Scion base cut in v-shape was stuck by 1% agar-agar solution into the vertical slit of rootstock. There was no any displacement and the rate of fused and further developed grafts was 95 percent. Agar-agar between the rootstock and scion made the transport of different materials possible and hold the graft units together until the fusion took place. Fusion was proved also by histological studies. Some of in vitro micrografts were planted and acclimatisated and the survival was 100 percent.

  • Estimating of water consumption of cherry trees
    15-17.
    Views:
    175

    Despite of its importance there is no exact information on water use of new scion/rootstock composite trees, which would be needed to optimized irrigation. Our research purpose is to define exact water-demand of different rootstock/scion composite trees, calculating seasonal weather changes and by using the results decrease irrigation costs. The investigations are carried out in Soroksár, at the Experimental Farm of Corvinus University of Budapest in May 2008. From among the investigated trees two are budded onto Prunus mahuleb `Érdi V' seedlings, two on "Korponay' seedlings. The sapflow measurements are carried out using Dynamax Flow 32 equipment with Dynagage trunk sensors. The first daily maximum of sapflow was around 10:00 a.m. (2.5 kghour I), the second maximum was always between 14:00-15:00 p.m. (2 kgday- I). Comparing to the very intensive morning water uptake by 20:00 p.m. the water flow slowly reached the minimal level. Significant differences can be seen on rootstocks: trees on `Korponay' rootstock always showed more intensive sapflow and a higher morning peak than trees on `Árdi V'. But later during the day they have the same run.Based on our results the water quantity transpired only by the trees reached in May 86-104 mm, while the precipitation was only 42.4 mm. This means a 40-60 mm deficit in the orchard, which should have been supplied by irrigation despite of the satisfying horticultural performance of the orchard. In the first half of the month beside the steady vapor pressure deficit the shoot and leaf surface growth could cause the increased sapflow.

  • Flowering performance of some Modern Rose Varieties in Hungary
    69-75.
    Views:
    132

    A variety trial has been accomplished to study the flowering performance of some modern roses. Blooming time and blooming intensity were studied in Hungarian and Western European varieties. 120 Floribundas, polyanthas and climbings were observed. Our work shows that valuable Hungarian varieties can be found in all the three studied classes. The best Hungarian climbing roses at blooming intensity were `Futótűz', `Rozalia', ‘Sarolt and 'Szent Erzsébet emléke'; best floribundas were ‘Báthory István emléke', 'Munkács', 'Szent Margit emléke; and the best Hungarian polyanthas were `Csinszka', `Domokos PáI Péter emléke'. Some really good flowering Western-European rose varieties have also been found, the best ones were 'Clg. Gertrude Westphal' climbing, 'La Sevillana' floribunda and 'Beauty of New South Wells', 'Happy' polyanthus. 'La Sevillana' and 'Picasso' were in strong bloom for the longest time.

    In Hungary, the floribunda and polyantha classes had good flowering intensity to the almost the same extent, floribundas had stronger, and polyanthas had longer flowering waves, but the ever-blooming ability of the climbing roses was moderate in the dry midsummer.

  • Using compost of grape manufacture and farm wastes as growing media in vegetable ad ornamental nurseries
    45-50.
    Views:
    216

    This work was conducted at private nursery in Kafr El-Sheikh governorate during 2006 and 2007 seasons to investigate the possibility of using grape manufacture waste compost (GMWC) and farm wastes compost (FWC) in ornamental and vegetable nurseries as partially or totally replacement of coconut peat (CP) and vermiculite (V) in the growing medium and also to find out the optimum media of tomato (Lycopersicon esculentum, cv. Castle Rock) and Cockscomb (Celosia plumosa) as comparing to a mixture of CP and V (1:1 v/v). The authors used a ten mixtures as followed: 1- Control (CP+V at 1:1 v/v), 2-GMWC (100 %), 3- GMWC +CP (1:1 v/v), 4- GMWC + V(1:1 v/v),5- GMWC +CP + V (1:1:1 v/v/v), 6- FWC (100 %),7- FWC+ CP (1:1 v/v), 8- FWC+ V (1:1 v/v), 9- FWC+ CP+ V (1:1:1 v/v/v), 10- GMWC+FWC+CP+V (1:1:1:1 v/v/v/v). Data recorded as seedling height, No. of leaves, total pigments, shoot fresh and dry weights, root length and root fresh and dry weights in order to assess the quality of both transplants of tomato and cockscomb. Both seedlings grown in medium contain a mixture of GMWC+CP+V displayed quality traits similar or better as to those of recorded from the control treatment.