Vol. 7 No. 1 (2001)
Articles

Results in the determination of some Hosta varieties by the method of isoelectric focusing

Published March 21, 2001
A. Mándy
Univ. of Horticulture and Food Dep. of Floriculture and Dendrology, 1118 Budapest, Villányi út 35-43., Hungary
É. Stefanovits-Bányai
Univ. of Horticulture and Food Dep. of Chemistry and Biochemistry, 1118 Budapest, Villányi út 35-43., Hungary
Cs. Imre
Univ. of Agricultural Science Dep. of Horticulture, Gyöngyös, Mátrai út 2., Hungary
Zs. Szafián
PRENOR Nursery, 9707 Szombathely, Béke tér 1., Hungary
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APA

Mándy, A., Stefanovits-Bányai, É., Imre, C., & Szafián, Z. (2001). Results in the determination of some Hosta varieties by the method of isoelectric focusing. International Journal of Horticultural Science, 7(1), 90-92. https://doi.org/10.31421/IJHS/7/1/256

Mass propagation of 5 newly introduced Hosta varieties was carried out by the method of micropropagation. Our aim was to determine exact variety specificity after the micropropagation period in the pattern of peroxidase isoenzymes by isoelectric focusing in pH 3-9 range and to determine that phenological phase of mother plant in which the isoenzyme pattern of mother plant can safely be comparable to the isoenzyme pattern of micropropagated descendants. The isoenzyme patterns of descendants were similar to the mother plants of the same hybrid lines. The older leaves seemed to be not so suitable for examination than newly developed ones despite of the higher activity of peroxidase enzymes. There were big differences in isoenzyme patterns of leaves in different phenological phases. With this quick and easy method Hosta varieties could be selected already in the very early stage of micropropagation.

 

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