Search
Search Results
-
The role of pruning in the intensification of plum production
83-86.Views:233In an orchard planted in the spring of 1997, four kinds of spacing have been applied (4.0 m x 1.5 m, 4.0 m x 2.0 m, 5.0 m x 2.5 in and 6.0 m x 3.0 m). Four cultivars (‘Cacanska lepotica', Stanley' ‘Bluefre' and ‘President') grafted on Myrobalan rootstock were studied in the experiment aimed to explore the performance of plums under different spacing and training conditions.
It could be stated that the trees grown on vigorous rootstock are prone to be cultivated much more intensely (smaller tree size, higher tree density) by the consequent use of green pruning technique. The most favourable economic combination (yield and labour costs) was found to be: 4.0 in x 2.0 in and 5.0 x 2.5 in (800-1200 tree/hectare), whereas the most adapted was the cultivar: 'Stanley'.
-
Evaluation of generative accomplishment of new apple cultivars in Hungary
11-15Views:287In a two year study, 14 new apple cultivars (cvs ’Gala Venus Fengal’, ’Gala Decarli-Fendeca’, ’Galaval’, ’Jugala’, ’Gala Schnitzer (S) Schniga’, ’Red Cap Valtod (S)’, ’Early Red One’, ’Jeromine’, ’Crimson Crisp (Co-op 39)’, ’Red Topaz’, ’Wilton’s Red Jonaprince’, ’Red Idared’, ’Fuji September Wonder’ and club cultivar) were studied in Eastern Hungary at Nyírbátor. The following parameters were measured: trunk cross sectional area (cm2), harvest time with yield (date, kg/tree, number/tree), fruit size (mm), fruit surface color (%), and color intensity of the fruit surface (1-5). According to our two year assessments regarding, the best generative accomplishment was achieved for the cvs ‘Gala’ sorts trained to slender spindle and the cv ‘Red Jonaprince’ trained to super spindle. According to the trunk cross sectional area the ‘Gala’ sport trained to slender spindle showed the strongest growth among the 4 years old trees, while the cv ‘Fuji SW’ and the cv ‘Jeromine’ presented lower vegetative accomplishment. The ripening time of the cultivars started at the end of August and finished at the beginning of October. ‘Gala’ mutants trained to slender spindle provided the highest yields. Fruit size of all cultivars reached the market required standard, however, cvs ‘Red Jonaprince’ and ‘Red Idared’ produced large fruits over 90 mm. The fruit size of the ‘Gala’ sort was 72–75 mm. Most of the cultivars showed high fruit surface color and color intensity.
-
Relationship beetwen the phenological features of pear cultivars and the main meteorological parameters in a gene bank with 555 pear
59-63.Views:202The trees observed are grown at Ujfehert6, Eastern Hungary in a gene bank with 555 pear cultivars. Each of the cultivars was monitored for its dates of: the beginning of bloom, main bloom and the end of bloom and ripe phenophasis separately between I 984 and 2002. We analyzed the statistical features, frequency, distribution of these phenophasis and its' correlation the meteorological variables bet ween the interval. During this period the meteorological database recorded the following variables: daily mean temperature (°C), daily maximum temperature (0C), daily mini m um temperature (0C), daily precipitation (mm), daily hours of bright sunshine, daily means or the differences between the day-time and night-time temperatures (0C). For the analysis of data the cultivars have been grouped according to dates of maturity, blooming period as well as types of the seasons. Groups of maturity dates: summer ripe, autumnal ripening, winter ripe cultivars. Groups of blooming dates: early blooming, intermediate blooming, late blooming cultivars. At all the separated groups we analyzed the relationship between phenophasis and meteorological variables. During the 18 years of observation , the early blooming cultivars started blooming on 10-21 April, those of intermediate bloom date started flowering bet ween 20 April and 3 May, whereas the late blooming group started on 2- 10 May. Among the meteorological variables of the former autumn and winter periods, the winter maxima were the most active factor influencing the start dates of bloom in the subsequent spring. For the research of fruit growing-weather relationships we used simple, well known statistical methods, correlation and regression analysis. We used the SPSS 1 1.0 software for the linear regression fitting and for calculation of dispersions as well. The 1ables made by Excel programme.
-
The inheritance and durability of scab resistance in apple progenies
39-46.Views:142In order to select the appropriate parent cultivars and maintain the durability of resistance, it is important to clarify the mechanisms of inheritance of scab resistance depending on the parents. It has been known that the progeny segregation ratios based on scab-resistance do not depend only on the genotype of the resistance locus but also on the genetical makeup of the donor and recipient parents as well as on the susceptible parent.
The aim of this study has been to demonstrate what factors in the Vf, Vr and VA scab-resistant cultivars — combined with susceptible and resistant parents — affect the inheritance and durability of resistance in seedlings in their first 4 year's growing four years' growth. After inoculating apple seedlings sown in 2001 with the suspension of Venturia inaequalis (Cke.) Wint. in the greenhouse, we studied the segregation ratios of the progenies into reaction classes. Seedlings showing resistance in the greenhouse were also evaluated for scab-resistance after they had been moved to the field and had naturally been infected with the pathogen in 2002 and 2004.
The majority of our results obtained in the greenhouse test, similarly to earlier experiences, have not justified monogenic inheritance at the phenotypic level. The effect of susceptible parent cultivars on the segregation ratio of progenies have become apparent again. The high infection rate of seedlings in the field trials, which had previously exhibited varying degrees of resistance in the greenhouse test, has raised concern. Our data has raised further doubts, concerning the durability of Vf resistance in Hungary. It is assumed that the composition of natural field populations of Venturia inaequalis in Szigetcsép has changed. The complexity of Vf resistance has been confirmed. The high infection rate in the progenies derived from Vf resistant cultivars draws the attention to the importance of utilizing additional sources of resistance.
-
Molecular analysis of strawberry cultivars using RAPD, AP-PCR and STS markers
24-28.Views:127Seventeen strawberry (Fragaria x ananassa Duch.) cultivars representing the national list of Hungary, were subjected to RAPD, AP—PCR and STS analysis. Of the 31 decamer and oligomer primers tested 26 primers produced polymorphic patterns. 45 polymorphic fragments were analysed, ranging between 200-2800 by in size. Based on the data, similarity coefficients (Jaccard index and Simple matching coefficient) were calculated, and dendrograms were constructed using the unweighted pair group method of arithmetic averages (UPGMA). The dendrograms only partly reflect the known pedigree data. Specific RAPD markers were identified for cultivars F5, Pocahontas and Rabunda.
-
Preliminary evaluation of breeding perspectives of Ukrainian sweet cherry cultivars: nutraceutical properties and self-incompatibility
7-11.Views:332Some traditional sweet cherry cultivars of Ukrainian origin may represent perspective material for Hungarian cherry breeding. A total of eight cultivars analysed represent great diversity in several phenotypic traits including fruit ripening time or fruit flesh colour. Considerable differences in the anthocyanin content may result in different antioxidant capacity of fruits. In the present study, we used ferric reducing antioxidant power (FRAP) and total phenolic content (TPC) assays to characterize fruits’ nutraceutical properties. These values were compared with the respective values measured for eight commercial cultivars grown in Hungary. The average of FRAP and TPC values was higher for the Ukrainian cherries compared with commercial cultivars suggesting they might be included in functional breeding programs. Since, cherry is a self-incompatible species, the determination of S-genotype is required for both breeding and successful cultivar association in commercial orchards. Complete or partial S-genotypes were determined for 5 and 3 cultivars, respectively.
-
RAPD analysis of grapevine hybrids and cultivars
63-66.Views:155Utilization of the Randomly Amplified Polymorphic DNA (RAPD) technique as a molecular marker was tested to investigate the relationships between some representative grapevine cultivars and hybrids established at the Department of Genetics and Plant Breeding (CUB), to distinguish clones as well as to characterize various hybrids between species or cultivars and their parents. Vitis vinifera cultivars were easily and successfully distinguished by the RAPD technique and they were grouped according to the traditional taxonomic classification. RAPD patterns of the examined Pinot gris clones proved to be completely identical. Number of generations was reflected by the value of genetic distance of the examined hybrids. Genetic identity of parents and their offsprings was influenced by the selection applied in the process of plant breeding. Parental phenotypic and morphologic characteristics showed high degree of segregation in hybrids, but RAPD analysis revealed that their genetic similarity is considerable. The three Vitis anntrensis clones were properly discriminated from every cultivar and hybrid of Vitis vinifera, i.e. hybrids are much closer to the cultivated grapevine than to V. anzurensis due to the phenotypic selection carried out during the life-cycle of one or two generations.
-
Some flower characters, variety features and environmental effects affecting bee pollination of pear (Pyrus communis L.): a review of the results of latest research
59-66.Views:175Intensive research has been implemented on different aspects of the bee pollination of pear in Hungary in the past decade, extending to the following topics: the effect of the opening sequence of the flowers within the inflorescence of pear cultivars on the effectiveness of bee pollination; the nectar production of flowers of pear cultivars; intensity of honeybee visitation and their foraging behaviour at the flowers of pear cultivars; flower constancy of honeybees to pear plantations; competition by weeds in bloom for bee pollination in flowering pear orchards; and some aspects to the bee pollination strategies of pear plantations (the effect of the limitation of bee pollination period on the set and the yield, possible compensation of frost damage of flowers by bee pollination in pear orchards, the optimal size of single cultivar block in pear plantations). The results of these studies are reviewed in this paper and some important aspects that need further studies are outlined.
-
Simultaneousness of reproductive organs of sour cherry cultivars adaptable to Iranian climatic condition
25-31.Views:226Observations were made on simultaneousness of pollen shedding and stigma viability ratio, to clarify the phenology of reproductive organs of nine economically important sour cherry (Prunus cerasus L.) cultivars (‘Érdi bôtermô‘, ‘Debreceni bőtermő‘, ‘Csengődi‘, ‘Kántorjánosi 3‘, ‘Újfehértói fürtös‘, ‘Petri‘, ‘Eva‘, ‘Oblascinszka‘, and ‘Pándy 279’). The main aim was investigation the possible influence of environmental cues in timing and development of phenophases and also responses of different cultivars to seasonal cues. Likely effects of climatic changes on phenological development patterns were also considered. Results showed important aspects of the reproductive biology of sour cherry flowers. The amplitude of the phenophase “beginning of blossoming” between cultivars did not exceed 6 days. There is a maximum 3 day difference in the blooming length of different directions in each cultivar. ‘Pándy 279‘ showed high variability when the position of flowers changed on the tree shoots. ‘Újfehértói fürtös‘ was stable in all four directions of the tree. The pollen shedding period and stigma viability ratio were synchronous. Pollen shedding phenomenon simultaneously occurred with the secretary activities of stigmas in all examined cultivars. Distribution of pollen shedding over the secretary activity of stigmas is almost good. In all five cultivars maximum pollen shedding occurred when the temperature was high during the day. In general the results of this experiment and our previous studies clearly demonstrate that the Pándy groups not recommended for plantation in new orchards of Iran.
-
Comparative evaluation of chamomile (Matricaria recutita L.) populations from different origin
91-95.Views:393In our research project under cultivation we examined 4 cultivars ('Soroksári 40', 'Lutea , 'Goral', 'Bona') and 28 wild populations of chamomile assuring all of them the same environmental conditions. Plant height, flower-diameter, essential-oil content, and the main terpenoid and flavonoid composition were analysed. The aim of our study was to establish the genetic background of breeding a new cultivar as well as encircle those natural habitats that give chamomile drug with the best quality.
In the case of plant height populations from the Great Hungarian Plain were lower than plants from Transdanubia and the control cultivars. Between the wild and the standard individuals we found significant differences with relevance to the flower-diameter. With regard to the essential oil content the populations were very heterogeneous even those, which came from the same habitat. Populations form Transdanubia and Nagyiván reached the essential oil level of the cultivars (0.721-0.931 gi100g), and 75% of the examined plants exceeded the minimum requirement of the PhHg VIII.
According to the essential oil composition our previous statement was confirmed that in the populations of Transdanubia and Northern part of Danube—Tisza Mid Region the main component is bisabolol-oxide A (30-41.2%), while plants native to the territory cast of the river Tisza are mainly characterised by a-bisabolol (32.3-48.4). In some samples the ratio of bisabolol-oxide B was more than 10%. The chamazulene content was higher in the cultivars selected to this component (above 20%), than in the wild populations (varied between 1.22 and 17.2%). Populations originated from the central part of Hortobágy region had extremely high apigenin content (10-13 mg/g), but in the case of chlorogenic acid, hyperoside and quercitrin we did not find any differences affected by the origin.
-
Effect of life cycle on the production of mullein (Verbascum phlomoides L.)
123-125.Views:156Aim of the present investigations was the optimalization of the production of the annual cultivar `Napfény' of Verlxiscum phlomoides L. Quantitative data on morphology (growth, leaf and flower size, branching) yield and content of active materials (mucilages, flavonoids) were studied at six sowing times.
We established, that sowing time may be one basic factor in the production of the annual variety. The major yield was obtained by sowing either late autumn (end of October) or early spring (middle March). At these plots the fresh mass of the flowers was 257-288 g/plant, the drug mass 28-29 g/plant, by 45-70% more than that of the mean of other treatments.
It was established, that under optimal cultivation conditions the annual form of mullein may reach higher individual yields than the plants of the indigenous wild growing population.
-
Determination of the cold tolerance off sour cherry cultivars with frost treatments in climatic chamber
49-54.Views:234Nowadays, sour cherry buds can be seriously damaged by spring and winter frosts. Unlike other fruit species threatened by high frost damage, sour cherry cultivars have not been assessed for frost tolerance. The aim of .our survey was to establish the relative cold tolerance of the Hungarian cultivars after treatment in a climatic chamber, and to optimize the methodology formerly elaborated for the frost treatment of apricot. Fourteen cultivars of Hungarian sour cherry (Prunus cerasus) were used in the experiments, which spanned the winters of 2005/2006 and 2006/2007. Our data were used to rank cultivars in two groups according to their levels of cold resistance. We also recommend critical temperatures and treatment times for the testing of sour cherry cultivar resistance to cold in climatic chambers.
-
Genotyping Hungarian apricot cultivars for self-(in)compatibility by isoelectric focusing and PCR analysis
69-72.Views:159Self-incompatibility (SI) in flowering plants is a widespread genetic system that promotes out-crossing. In Prunus species the SI is a gametophytic trait, which is controlled by a single multiallelic locus, termed S-locus. S-alleles codify stylar glycoproteins with ribonuclease activity (S-RNases). Our objective was to assess the S-genotype of some Hungarian apricot varieties by isoelectric focusing of stylar RNases as well as by PCR analysis using cherry consensus primers. Consensus primers amplified one or two bands of various sizes. Primers amplifying the 1st intron gained fragments the size of which ranged from 250 to 500 bp; while those amplifying the 2nd intron resulted in fragments of 800-2000 by length. Our data demonstrated that the first intron of the apricot S-RNase gene is shorter than the second one, which coincides with the structure of cherry S-RNase alleles. `Hargrand' (S1S2) and `Harcoe (S1S4) possessed one common S-RNase isoenzyme. Hungarian 'Orias' apricot cultivars showed different bands compared to the previous cultivars, but they shared completely identical patterns confirming that they possess the same S-genotype. 'Bergeron', `Harmat' and 'Korai zamatos' are characterised by an evidently distinct S-RNase pattern. The self-compatible cultivar (`Bergeron') had one allele, which suggests its correspondence to the Sc. Primers for the 2nd intron was unsuccessful in gaining fragments, which indicates that the 2nd intron in the Sc allele is too long to get any amplification. On the basis of our data, identities and differences were revealed in the S-allele constitution of some economically important Hungarian apricot cultivars at protein and DNA levels.
-
Disease threshold for cherry leaf spot incidence on commercial sweet cherry cultivars
52-54.Views:233The aim of this study was to demonstrate the 10% threshold level for cherry leaf spot incidence on 23 commercial sweet cherry cultivars in two training systems. Twenty three cherry cultivars were evaluated in the two training systems with a spacing of 4 x 1 m and with a spacing of 2 x 5 m. Results showed that leaves of many cultivars were heavily infected, e.g. cultivars (cvs) ’Biggareau Burlat’, ’Sunburst’, while others showed low disease incidence e.g. cvs ’Celeste’ and ’Blaze Star’. According to the 10 % threshold level, cv ’Celeste’ proved to be the most resistant cultivar to leaf infection, while cv ’Münchebergi korai’ exceeded extremely the 10% threshold level. Leaf spot incidence was affected by training system on most cultivars. Thirteen cultivars had less than 10% infection in the 4 x 1 m spacing (i.e. they did not reach the 10% disease threshold level). At the same time, only 3 cultivars showed less than 10% infection in the 5 x 2 m spacing.
-
Response of alternate bearing of “Badamy sefid” Pistachio cultivar to foliar application of some macro and micro elements
109-112.Views:223Response of “Badamy sefid” Pistachio trees to spraying of fertilizer was studied over two years (2006–2007). The boron and zinc fertilizer were used just on swelling time of female flower bud in compare to no fertilization (control). Also nutritional solution combined of nitrogen (400 ppm), phosphorus (P2O5) (380 ppm), potasium (K2O) (520 ppm), Fe (5 ppm), Cu (2 ppm), Zn (2 ppm), Mn (2 ppm) in 1000 liter water per hectare sprayed at first week of May, third week of June and July). The concentration of some macro and micro elements in flower bud and in leaves, also productivity (g/shoot sectional area) mean nut weight, fresh weight of nut per cm2 branch cross sectional area, blanks nuts %, non-split nut %, new shoot growth length after cessation of growth and remained flower bud (%) at the next spring were recorded for the different treatments. The results showed that boron and zinc concentration increased in the bud of sprayed trees in compare to control. Application of nutritional solution decreased the flower bud abscission, also resulted in increased vegetative growth, nut weight, and productivity of trees. In conclusion, spraying of fertilizers in the suitable time to the pistachio trees might be a useful method in decreasing flower bud abscission and mitigating the alternate bearing.
-
Evaluation of the vegetative and generative performance of new apple cultivars in the Nyírség region
39-44.Views:231In this paper we examine apple varieties, which are planted expansively in the development apple orchards of the Western- European countries, but about its production characteristics we don’t have or just few practical experiences in Hungary. These varieties can be described with regular and high yields, aesthetic and alluring appearance in their origin places, what ensure profitable producing for the growers. Under the Hungarian climate which tends to be often extreme (hard cold winters, late spring frosts, summer drought, heat stress) these varieties may behave differently, so before the substantial planting it is expedient to carry out variety evaluations to avoid the later failures. The place of our experiments is located in the Nyírség representing adequately the ecological conditions of the surrounding areas, so the gained results can be adapted easily in the biggest apple production site of Hungary. Regarding the vegetative and the generative parameters of the 14 examined varieties (’Gala Venus Fengal’, ’Gala Decarli-Fendeca’, ’Galaval’, ’Jugala’, ’Gala Schnitzer (S) Schniga’, ’Red Cap Valtod (S)’, ’Early Red One’, ’Jeromine’, ’Crimson Crisp (Co-op 39)’, ’Red Topaz’, ’Wilton’s Red Jonaprince’, ’Red Idared’, ’Fuji September Wonder’, club cultivar) we found significant differences. The ratio of the thickness of trunk and the main branches showed that the trees have an optimal canopy structure in accordance with the Zahn principles, so they are in harmonious growing balance. The ‘Gala’ sport trained to slender spindle produced the highest yields, while the ‘Fuji’ and the ‘Red Delicious’ sport trained to super spindle can be described with the lowest values. All the cultivars reached the eating quality (65-70 mm), from which the ’Red Idared’, the ’Red Jonaprince’ and the ’Red Cap’ produced outstanding fruit size (81-85 mm).
-
Fire blight (Erwinia amylovora) resistance in apple varieties associated with molecular markers
53-57.Views:230The invasive bacterial disease fire blight, caused by Erwinia amylovora has the potential to destroy fruit tree orchards all over Europe. Effective plant protection methods are lacking in many countries, highlighting the increasing importance placed on identification of germplasm with heritable disease resistance. Recent l y. a promising QTL (quantitative trait locus) was identified on linkage group 7 in the apple cultivar 'Fiesta· which is derived from ·cox's Orange Pippin' . I n the present study, 144 Swedish and foreign apple cultivars were analysed with the SCAR markers AE I 0-375 and GE-8019. which flank-. this QTL. Twenty-nine of the analysed cultivars had both markers. 78 had either AE I 0-375 or GE-8019, and 37 cultivars did not carry an) of the two markers. Seventeen cultivars. 7 with both markers and I 0 not having either of the two markers, were then inocoluted with the bacterium in a 4uaran1i ne greenhouse test. Cultivars carrying both DNA markers were significantly less susceptible than cultivars lacking the markers, P<0.001. Cultivars that were most resistant had both markers and had 'Cox· in their pedigree. Unrelated cultivars with the markers may still lack the QTL.
-
Fire blight (Erwinia amylovora) susceptibility of old Hungarian apple cultivars
35-38.Views:152The aim of the Hungarian apple breeding program started in the Department of Fruit Science was to find resistant apple cultivars against major diseases (scab, powdery mildew, fire blight). The outbreak of fire blight (Erwinia amylovora) in 1996 motivated us to search new resistant sources principally from old traditional apple cultivars. First of all, cultivars have been gathered since 1997 from Carpathia (Visk) and evaluated between 2001 and 2003. In this recent study evaluation of resistance of old Hungarian genotypes to fire blight collected from the English National Fruit Collection (Brogdale, Faversham) is presented.
13 old Hungarian apple cultivars in 2002, and 38 genotypes in 2005 have been evaluated. We used `Idared' and 'Jonathan M41' as susceptible controls and 'Liberty' and 'Remo' as resistant ones. Shoots of two-year-old potted plants were inoculated with a mixture of virulent E. amylovora isolates (Ea2, Ea60, Ea67) at a concentration of 5 x 108 cells/ml. Resistance of apple cultivars was evaluated weekly, four times after inoculation by disease severity of symptoms. Numbers of bacterial colonies in 1 cm length shoot were determined in the fourth week after infection.
8 cultivars in 2002 and 9 cultivars in 2005 displayed notable resistance to fire blight based on one-year data. Based on the coincident data of both years, out of the cultivars collected also from Carpathia ‘Pónyik', and `Sikulai' were found to be resistant and gene sources additional old Hungarian valuable apple genotypes could be selected: `Szabadkai szercsika' and `Tordai piros !davit'. The cultivar `Szemes alma' originated from Visk has proved to be recurrently resistant.
The number of bacterial cells in shoots of the investigated cultivars correlated with the severity of symptoms. With this method, which was introduced by us earlier, we can screen cultivars displaying weak visible symptoms, which cannot be proposed as a source of resistance because of their latent infection.
-
The selection and cultivation of Tilia clones tolerating polluted urban environment
82-86.Views:215Two urban stress tolerant Tilia clones were selected by the Department of Floriculture and Dendrology. The mother trees of the Observed clones were found in an alley of linden trees, settled on a traffic island of a busy two-lane road.
Tilia hybrid 'Saint Stephen' has a beautiful cone-shaped crown, the leaves are bright green and they keep their green colour for much longer time than the leaves on the other trees in the alley. In the nursery the Tilia hybrid 'Saint Stephen' was budded on T. cordata, T. platyphyllos and T. argentea and it had good compatibility with every rootstock. The average height of the one year old buddings was 200 cm and the buddings kept their good growing capacity in the following years as well. They had an outstanding growing capability comparing with the other Tilia cultivars.
Tilia platyphyllos 'K3' clone has similar cone-shaped crown. The growing vigor and urban stress tolerance seems to be better than Tilia hybrid 'Saint Stephen'.
-
Comparative analysis of sour cherry cultivars on their ecological and biological indicators
7-28.Views:344Sour cherries developed in the northern hemisphere, an alloploid hybrid of dwarf sour cherries (Prunus fruticosa) and bird cherries (P. avium), born in the confluence of the two species. However, the ecological and, above all, cold tolerance of the ancestor of cultivated sour cherries is higher than that of wild cherries (De Candolle, 1894; Rehder, 1954; Terpó, 1974; Iezzoni et al., 1991; Faust & Surányi, 1997). The cultivation limits are in the northern hemisphere 38-44. degree. The Carpathian Basin, the Balkans and Asia Minor are considered to be the main birthplaces for sour cherries. The genetic and morphological diversity of sour cherries is greater than that of the basic species (Iezzoni et al. 1991; Faust & Surányi, 1997). In the study, 472 sour cherry cultivars were compared based on 7 relative ecological indicators and 3 biological values. Compared to other Prunus species, we mostly found less variability in sour cherries - not counting their salt tolerance (SB). The partial similarity between open pollination (OP), frost tolerance (FR) and disease resistance (DR) - partly true in terms of varieties, but also reflected the effects of purposeful breeding and selection. The cultivars together - in comparison, showed balance, but in the highlighting, the differences of the 3 cultivar groups became significant. Indeed, the differences between the species of the former Hungarian cultural flora are clearly different (Surányi, 2004), which is also the case when comparing a large number of apricot (Surányi, 2014), plum (Surányi, 2015) and peach (Surányi, 2020) varieties.
-
Comparative anatomical study of leaf tissues of scab resistant and susceptible apple cultivars
43-45.Views:182According to previous studies some anatomical features seem to be connected with resistance or susceptibility to scab caused by Venturia ineaqulis (Cke./Wint.) in case of a given cultivar. Study of leaf anatomy of three scab resistant (‘Prima’, ‘Florina’, MR–12) and two susceptible (‘Watson Jonathan’, ‘Golden Delicious Reinders’) apple cultivars have been made. Preserved preparations made of leaves has been studied by light microscope. Studied parameters were: thickness of leaf blade, thickness of palisade and spongy parenchyma, thickness of epidermal cells, thickness of the cuticle. By measuring leaf thickness and epidermal cell thickness visible differences appeared in certain cultivars, while most conspicuous difference has been shown in thickness of the cuticle.
-
Organic apple growing using sanitation treatments against apple scab
11-13.Views:141Effects or sanitation practices were evaluated on primary and autumn infection by Venturia inaequalis in an organic apple orchard at Eperjeske on the moderately scab-susceptible apple cultivar Jonathan in 2005 and 2006. Evaluated sanitation practices were: i) collection of fallen leaves in autumn; ii) destroying fallen leaves by disc cultivation in autumn; iii) spraying fallen leaves with 2% lime sulphur in autumn; iv) spraying fallen leaves with 2% lime sulphur in autumn and then collection of fallen leaves in autumn; and v) untreated control. In both years, most sanitation practices reduced significantly (P< 0.05) scab incidence in the primary infection periods compared to untreated control. The highest leaf scab incidence was observed in the untreated plots (26:2 and 24.3% in 2005 and 2006, respectively), while the lowest was in the treatments of spraying fallen leaves with 2% lime sulphur combined with collection of fallen leaves (11.7 and 12.3% in 2005 and 2006, respectively).
-
Old Hungarian grapevine cultivars and their relations characterized with microsatellite markers
27-31.Views:223Thirty-one old Hungarian grapevine (Vitis vinifera L.) cultivars were investigated on 7 microsatellite loci to characterize them, to separate the cultivars from synonym names, and to confirm parent-offspring connections. Conculta (group of cultivars or bud sports) members, such as `Goher' and Tajor' representatives, were studied to find a suitable locus for the separation. Synonyms, conculta members, subcultivars and clones of Turmine, which was the most important cultivar of Tokaj, were also analyzed to separate the members of the different taxonomic levels. Pedigree of 'Kiralyleanyka' was examined to find the missing ancestor, because the parent-offspring connection between the natural hybrid and `Koverszolo' is questionable.
-
The effects of growth regulators in proliferation of Sorbus redliana 'Burokvölgy'
77-83.Views:203The Hungarian cultivar Sorbus redliana 'Burokvölgy' was proliferated on Murashige and Skoog (MS, 1962) medium with half-strength macroelements and 100 mg/1 meso-inositol, 20 g/1 sucrose, 11 g/1 agar-agar. Different combinations of kinetin (KIN), metatopolin (mT), benzyladenine (BA), benzyladenine-ribosid (BAR) and indolebutiric acid (IBA) were tested, and pH was adjusted to 5.6 every case using KOH. The cultures were incubated at 20-24 °C in 8/16 hours dark/light photoperiod for 50-52 days. The main aim of our research was to find the optimal growth regulator and its optimum concentration. Purthermore, to determine the chlorophyll contents of the in vitro propagated plants' leaves. During the proliferation, the highest number of shoots were observed in the case of using BA + IBA, and on the medium containing 0.75 mg/I BA + 0.05 mg/1 IBA 8.93 shoots were found. The addition of KIN + IBA decreased the number of shoots and increased the sizes of leaves — the widest (11.2 mm) and longest (17.8 mm) leaves were obtained on the medium containing 1.00 mg/I KIN + 0.05 mg/1 IBA. The longest shoots (36.46 mm) were found in the case of applying 0.75 mg/1 BAR + 0.05 mg/I IBA. The BA + KIN + IBA combination resulted the shortest shoots. Sometimes not only shoot regeneration but spontaneous rooting was observed during the multiplication. The highest chlorophyll content (1.569 mg/g total chlorophyll, 1.132 mg/g chlorophyll-a, 0.437 mg/g chlorophyll-b) was obtained in the presence of 1.0 mg/I KIN + 0.05 mg/1 IBA.
-
Effects of different cytokinins on the shoot regeneration from apple leaves of 'Royal Gala' and 'M.26'
69-75.Views:213The effects of different types of cytokinins on the shoot regeneration from leaf explants of apple scion 'Royal Gala' and apple rootstock 'M.26' were evaluated. Regeneration media contained either thidiazuron, or 6-benzylaminopurine, or meta-topolin, or zeatin, or kinetin, or their N9-ribosides, respectively, in the concentration range 0.5 to 8.0 mg 1-1. Effects of 'these cytokinins were evaluated on the percentage of regeneration (R%) and that of vitrification (V%) and on the number of regenerated shoots per explant (SN). Organogenetic index (0I) calculated from these data was used for the evaluation of efficacy of cytokinins. The course of shoot organogenesis also was followed using stereomicroscope. Types and concentrations of cytokinins applied in the regeneration media influenced each parameter significantly and the regeneration answer was strongly genotype-dependent. The best regeneration (SN: 11.08, 01: 7.5) was achieved in `Royal Gala' by using TDZ in concentration of 0.5 mg 1-1 (2.271,1M). There was a clear relationship between the effect on the regeneration efficacy and the chemical structure of cytokinins considering classical cytokinins, namely N9-ribosides applied in less concentration than nonribosides have the same or best regeneration effects except for 6-benzylaminopurine riboside. However, similar relationship could not be detected in the case of 'M.26'. SN was the highest (3.22) using 6.5 mg 1-1 (18.2011M) 6-benzylaminopurine riboside or 8.0 mg 1-1 (21.44 µM) meta-topolin riboside (3.18). SN was not significantly lower (3.12) by using 2.0 mg 1-1 (9.08 1M) TDZ, however, OI was about half as big (0.63 compared to 1.29 or 1.74 with 6-benzylaminopurine riboside or meta-topolin riboside, respectively). 'Royal Gala' had higher organogenetic ability, than `M.26': 3.5-fold higher shoot number per explant and more than 4-fold higher organogenetic index was reached with this cultivar than with 'M.26'. Moreover, the similar developmental stage of shoots could be observed 3-5 days earlier than in 'M.26' and if explants of 'Royal Gala' were further cultured with 3 weeks, SN increased from 11.08 to 24.42 on TDZ-containing regeneration medium, which might suggest higher organogenetic ability, too.