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The after-effect of paclobutrazol on morphological characteristics of in vitro Narcissus poeticus ssp. radiiflorus plants
43-46.Views:197After different pre-culturing period (12, 23 or 34 days) on ½ MS medium with 1 mg l-1 paclobutrazol, 1 mg l-1 N6-benzyladenine and 0.1 mg l-1 1- naphthaleneacetic acid , 3 groups of Narcissus poeticus ssp. radiiflorus bulb scales were kept on the same medium without hormones. The results were evaluated monthly and the final one happened after 7 month. The best results were achieved due to the shortest pre-culturing period (12 days; Group 1), with 4.9 bulblets and 4.54% hyperhydricity. The result of the second treatment (pre-culturing period of 23 days; Group 2) was not different significantly but the number of bigger bulblet were higher (4.54 bulblets). After the longest pre-culturing period (34 days; Group 3), the number of bulblets was low (3.68) and more hyperhydricity (18.18%) was detected. The highest number of roots (13.91) was observed in this group
very likely due to the strong after-effect of paclobutrazol. -
Comparative organoleptic examination of apple varieties developed by different environmentally safe technologies
69-70.Views:178Ecological or biological growing techniques are generally recognised as actual trends in agricultural production. The objective of our experiments is a comparison of apple fruits grown alternatively by integrated versus ecological technology. In a collection of varieties, the possibility of the experiment was feasible. At the same time, a comparison of the popularity of existing varieties was also actual. The organoleptic consumer’s tests rated the varieties, and the comparison with instrumentally raised “objective” data could be compared with the subjective ratings, which did not coincide with each other. This phenomenon was revealed in the rating of acid-sugar harmony, where the internationally used quality index of Thiault produced a different range of the samples then the consumer’s test. The same contradiction was observed between ratings of firmness and the data raised by penetrometer.
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Effects of silicon in plants with particular reference to horticultural crops - Review article
95-105.Views:339Silicon (Si) has long been considered as non-essential element for plant’s growth and production. Numerous efforts are being made for the discovery of its beneficial effects with large scale studies laying foundation for new findings and hypotheses. Therefore, Si has been suggested to be a quasi-essential element due to its positive effects against biotic and abiotic stresses alike. Though Si is the second most abundant element in the soil profile, its availability to plants is limited to the form of monosilicic acid only. Besides, plants’ ability to take-up Si and use it in their physiological processes also depends on the available transporters associated with it. Thus, the present review covers uptake and transport of silicon in plants as well as Si mediated physiological processes, including mechanisms underlying induced tolerance against biotic and abiotic stresses with a particular emphasis on horticultural species.
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High antioxidant - and anthocyanin contents of sour cherry cultivars may benefit the human health: international and Hungarian achievements on phytochemicals
45-47.Views:448Evidence suggests that a diet with high fruit and vegetable consumption may decrease the risk of chronic diseases, such as cardiovascular diseases and cancer, and phytochemicals including phenolics, flavonoids and carotenoids from fruits and vegetables may play a key role in reducing chronic disease risk. Recent research has proved that sour cherry (Prunus cerasus L.) is a valuable natural source of some bioactive compounds important in human health preservation. According to the published data, the most important biological effects of sour cherry are connected — directly or indirectly — to their endogenous antioxidant behaviour as well as to their specific pattern of anthocyanin components. In the present work, we measured the total antioxidant capacity of some Hungarian sour cherry varieties in combination with their anthocyanin-, and vitamin-C content. In 2003, twelve clones were selected and grafted from a local sour cherry population called "Bosnyák" sour cherry grown in small home gardens and farms of the village Csengod (Great-Plain Region, South Hungary). Other Hungarian sour cherry varieties, i.e. cv. Újfehértói fürtos, cv. Érdi bőterrnő, cv. Debreceni bőterrnő, cv. Csengődi and cv. Kántorjánosi served as a control.
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Effect of boron nutrition on nutrient uptake and fruit quality of tart cherry in Eastern Hungary
45-48.Views:286Effect of the foliar boron (B) application on nutrient uptake and fruit quality of tart cherry (Prunus cerasus L.) was investigated from 2008 to 2009 on mature four favourite Hungarian tart cherry cultivars like ‘Oblacsinszka’, ‘Érdi bôtermő’, ‘Újfehértói fürtös’ and ‘Petri’. Tart cherry trees grown in Eastern Hungary (Újfehértó) on an acidic sandy soil with low B content. Trees were treated with B by foliar application (0.5% B) at full bloom and untreated with B served as a control. B sprays strongly affected on B content of inflorescences. However, B sprays had no consistent effect on summer leaf B status. In our experiment stronger year effect was observed at leaf B status than flower B status. The effectiveness of early spring boron applications are limited and mostly affected the flower B status only. Moreover, treatments had inconsistent effect on studied fruit inner parameters. Monosaccharides, vitamin C and organic acid contents of sour cherry were stronger affected by cultivars than applied treatments.
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Antioxidant capacity, total phenolics and mineral element contents in fruits of Hungarian sour cherry cultivars
59-64.Views:483Several epidemiological studies revealed that the consumption of antioxidant compounds and the risk of atherosclerosis, increased blood pressure or cancer are inversely proportional. Fruits of sour cherry contain a wide range of antioxidant compounds including melatonin, perillyl alcohol, ellagic acid, several flavonoids, polyphenolics, and anthocyanins. This study was carried out to survey the antioxidant power and mineral element content of seven commercial sour cherry cultivars and three cultivar candidates and to assess the influence of some external conditions on fruits' functional properties. Our analysis revealed nearly 5- and 2-fold differences between the lowest and highest antioxidant capacities and total phenolics content, respectively. Some cultivars (`Kántorjánosi' and ‘Újfehértói fürtös') and cultivar candidates (D, 'Petri' and 'Éva') showed outstanding antioxidant capacity and total phenolic content; in addition, mineral element content in fruits of the ‘Újfehértói fürtös' cultivar was also favourable. Redox parameters of fruits were influenced by the cultivation plot or fruit positions within the canopy in about half of the cultivars tested. Genetic background of cultivars forms the decisive factor in determining fruits' antioxidant capacity, although external factors may have also sizeable modifying effects. Enhanced functional properties of the fruit may also be further increased through breeding programs since considerable variation exists within the tested germplasm.
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Examination of nitrogenous compounds in Hungarian bio-musts
109-113.Views:328There has not been any research done on the musts from organic grapes. As a result of the special technologies in organic farming we can expect bio-musts to have a different quantitative and qualitative composition of nitrogen compounds from that of traditional musts. In our present essay we deal with the tests of nitrogenous compounds (assimilable nitrogen content, aminoacid, biogenic amine content) in musts from Hungary's bio-wine cellars. Our results show that the change in procedures has resulted in neither quantitative nor qualitative changes in the nitrogenous compounds of bio-musts.
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Comparative evaluation of chamomile (Matricaria recutita L.) populations from different origin
91-95.Views:451In our research project under cultivation we examined 4 cultivars ('Soroksári 40', 'Lutea , 'Goral', 'Bona') and 28 wild populations of chamomile assuring all of them the same environmental conditions. Plant height, flower-diameter, essential-oil content, and the main terpenoid and flavonoid composition were analysed. The aim of our study was to establish the genetic background of breeding a new cultivar as well as encircle those natural habitats that give chamomile drug with the best quality.
In the case of plant height populations from the Great Hungarian Plain were lower than plants from Transdanubia and the control cultivars. Between the wild and the standard individuals we found significant differences with relevance to the flower-diameter. With regard to the essential oil content the populations were very heterogeneous even those, which came from the same habitat. Populations form Transdanubia and Nagyiván reached the essential oil level of the cultivars (0.721-0.931 gi100g), and 75% of the examined plants exceeded the minimum requirement of the PhHg VIII.
According to the essential oil composition our previous statement was confirmed that in the populations of Transdanubia and Northern part of Danube—Tisza Mid Region the main component is bisabolol-oxide A (30-41.2%), while plants native to the territory cast of the river Tisza are mainly characterised by a-bisabolol (32.3-48.4). In some samples the ratio of bisabolol-oxide B was more than 10%. The chamazulene content was higher in the cultivars selected to this component (above 20%), than in the wild populations (varied between 1.22 and 17.2%). Populations originated from the central part of Hortobágy region had extremely high apigenin content (10-13 mg/g), but in the case of chlorogenic acid, hyperoside and quercitrin we did not find any differences affected by the origin.
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The effect of 5-aminolevulinic-acid (ALA) on the development of Saintpaulia ionantha
33-36.Views:302In recent work the effect of 5-aminolevulinic-acid (ALA) agent (commercial name Pentakeep-V) was examined on the chlorophyll content, growth and development of Saintpaulia ionantha. The newly re-rooted potted plants were irrigated or sprayed with 0.3‰ or 0.5‰ Pentakeep-V solution, and plus 30% long lasting fertilizer was added to half of the all treatments. Control plants were sprayed with tap water. Best result were obtained on the field of flowering. All the treatments promoted chlorophyll-content in the leaves especially spraying with 0.3‰concentration. Plants treated with Pentakeep-V in both concentrations and independently from the spraying or irrigation flowered more than two weeks earlier than control and those that got plus 30% fertilizer. Besides in the case of some treatments the diameter of leaf rosette, the number and surface size of leaves grew comparing to the control. The longlasting fertilizer had positive effect on the fresh weight but none of treatments had effect on the dry weight.
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Carbohydrate utilization of Erwinia amylovora in vitro
31-34.Views:188Nectar is a multi-component aqueous solution that promotes bacterial multiplication. The concentration of nectar in plant flowers is not stable since it is under the influence of environmental conditions, especially free moisture and relative humidity. Experiments were conducted with "artificial nectar" and directed along two lines: (1) determination of the optimal concentrations of carbohydrates for the growth of E. amylovora development (2) consumption of different carbohydrates besides basic sugars.
Solutions of "artificial nectar" were prepared in different compositions by changing the dominance of basic sugars (fructose — glucose —sucrose) in proportions of 2:1:1, 1:2:1, 1:1:2 and between concentrations of 10-0.6% (diluted with Basal minimum broth) in order to determine optimal conditions for the development of E. amylovora.
At a basic sugar concentration of 10% bacterial multiplication started and continued until I log degree (from 106 to 107 cfu/ml). At concentrations of 5% and 2,5 % cells developed with nearly the same kinetics (from 106 to 8x107 cfu/ml and from 106 to 9x107 cfu/ml, respectively). Multiplication was more pronounced and nearly the same at concentrations of 1.2 % and 0.6 % (from106 to 2x108 cfu/ml). At a basic sugar concentration 30% total sugars bacterial multiplication did not occur, while at 20 % it was negligible, not measurable photometrically.
At minimal concentrations of F, G, S (between 1-0.1 %) bacterial cells were still able to multiply, producing organic acids from sugars.
Our study showed that E. amylovora requires only a small amount of sugars (0.1%) for multiplication (acid production) while high concentrations inhibit multiplication. There was a negative correlation between sugar content and cell density. The optimal range of sugar concentration was at about 1%.
Effect of "less frequent carbohydrates" to E. amylovora multiplication was also determined using the API 50 CH strip. We could provide information on utilization of 39 carbohydrates by the bacterium at different categories as follows: Not utilized-, Slowly and weakly utilized-, Slowly and completely utilized-, Quickly and completely utilized carbohydrates. We suppose that carbohydrates that belong to the latter two groups could play an important role as nectar components in promoting E. amylovora multiplication in the blossoms of pome fruit trees.
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Vegetative and micropropagation potential of Piper guineense (Schumach and Thonn)
29-36Views:189The continuous loss of forest plants due to deforestation, and the increasing demand for Piper guineense because of its medicinal and food value, has put a permanent pressure on its population in the wild where it is collected. A method for conservation and mass propagation is therefore required. This research was undertaken to determine the optimal concentration of auxin needed for vegetative propagation and to investigate the potential of Piper guineense for micropropagation. The auxin optimization study of vegetative propagation was based on the use of two-nodal stem cuttings treated with five different concentrations of indole-butyric acid (IBA). Growth parameters such as the number of sprouted, rooted and survived cuttings among others were determined. To investigate the potential of Piper guineense for micropropagation, nodal explants were subjected to different sterilizing treatments using ethanol, NaOCl, mancozeb, streptomycin and Plant Preservative Mixture (PPM). The effect of plant growth regulators (PGRs) was tested on sterilized nodal explants using full strength Murashige and Skoog (MS) hormone-free media alone as control and MS media supplemented with PGRs (BA, NAA and KIN) at different concentrations and combinations. Significant differences were observed across the treatments for all growth parameters measured. However, 2000 ppm IBA significantly (p<0.05) influenced sprouting and rooting of the stem cuttings. Piper guineense explants have deep tissue contaminants, which cannot be eradicated by surface sterilization alone except double sterilization using PPM. On control media, neither shoot nor root response was observed while the highest percentage of induced roots was obtained from explants cultured on MS +1 mg/L BA + 0.25 mg/L NAA. Shoot induction was only achieved when BA was used alone and when subcultured on media supplemented with NAA, which generated roots.
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Relaco: a product to reduce water shoot formation around pruning wounds in pome fruit trees
30-31.Views:114Larger cuts made during the winter pruning of pome fruit orchards a number of serious problems: the wounds serve as entrances for several branch-infecting pathogens, the numerous water shoots formed around the cut edges consume assimilates, shade the developing fruits, make the canopy dense, reduce the effectiveness of the pest management and cause extra work during next seasons pruning work. These problems can be eliminated by using proper wound protectants, like Relaco, a new Romanian wound protectant to cover larger pruning cuts of apple, pear and quince trees.
The treaments by Relaco resulted in a significant decrease in both the number and total length of water shoots formed around the cut egdes.
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Effect of basal and foliar application of boron and zinc on yield attributes in Solanum melongena L.
97-99.Views:139Field experiments were conducted during 1999-2000 and 2000-2001 to study the effect of basal application (BA) of zinc sulphate (5 and 10 mg kg-i soil) or boric acid (1 and 2 mg kg-1 soil) alone or foliar applications (FA) of water or 1% ZnSO4 or 1% H3 B03 or the combinations of both BA and FA of either ZnSO4 or H3 B03 on yield attributes (number of flowers and fruits/ plant, fruit weight, size and total yield). On the basis of the two-year data, it was found that all the treatments of BA as well as FA enhanced yield attributes but the combination of BA of 10 mg kg--1 soil ZnSO4 and FA of 1% H3 B03 gave highest yield as compared to other treatments and control plants.
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The effects of growth regulators in proliferation of Sorbus redliana 'Burokvölgy'
77-83.Views:217The Hungarian cultivar Sorbus redliana 'Burokvölgy' was proliferated on Murashige and Skoog (MS, 1962) medium with half-strength macroelements and 100 mg/1 meso-inositol, 20 g/1 sucrose, 11 g/1 agar-agar. Different combinations of kinetin (KIN), metatopolin (mT), benzyladenine (BA), benzyladenine-ribosid (BAR) and indolebutiric acid (IBA) were tested, and pH was adjusted to 5.6 every case using KOH. The cultures were incubated at 20-24 °C in 8/16 hours dark/light photoperiod for 50-52 days. The main aim of our research was to find the optimal growth regulator and its optimum concentration. Purthermore, to determine the chlorophyll contents of the in vitro propagated plants' leaves. During the proliferation, the highest number of shoots were observed in the case of using BA + IBA, and on the medium containing 0.75 mg/I BA + 0.05 mg/1 IBA 8.93 shoots were found. The addition of KIN + IBA decreased the number of shoots and increased the sizes of leaves — the widest (11.2 mm) and longest (17.8 mm) leaves were obtained on the medium containing 1.00 mg/I KIN + 0.05 mg/1 IBA. The longest shoots (36.46 mm) were found in the case of applying 0.75 mg/1 BAR + 0.05 mg/I IBA. The BA + KIN + IBA combination resulted the shortest shoots. Sometimes not only shoot regeneration but spontaneous rooting was observed during the multiplication. The highest chlorophyll content (1.569 mg/g total chlorophyll, 1.132 mg/g chlorophyll-a, 0.437 mg/g chlorophyll-b) was obtained in the presence of 1.0 mg/I KIN + 0.05 mg/1 IBA.
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Evaluation of sour cherry varieties grown with environmental technology
39-41.Views:592The evaluation of a produce is an important moment of predicting its success on the market. The general impression, which is decisive, when a consumer chooses to purchase the commodity, should be interpreted in more objective, measurable terms. Primary data have been collected on fresh fruits derived from different growing technologies by organoleptic tests and the data filled up in the forms are processed with correlation analysis. The components were: taste, aroma, flesh firmness. The coefficients of correlation showed that the rest of characters as the appearance, juiciness, and the sugar/acid ratio.
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Floral biological studies on pear cultivars in relation to fire blight susceptibility
25-30.Views:184Floral biological characteristics that may influence cultivar susceptibility to fire blight were studied in 10 pear cultivars in two Hungarian orchards from 1999 to 2003. The receptaculo-ovarial, automorphic nectary is usually bigger in tolerant cultivars than in susceptible ones. Nectary stomata are meso- or xeromorphic. Susceptible cultivars tend to have more xeromorphic stomata, where guard cells are located 1-3 cell rows below the epidermis. The size of nectar chambers is usually smaller in susceptible cultivars. Floral nectar, consisting mainly of glucose and fructose, is more abundant and less concentrated if the cultivar is susceptible to fire blight. The amount of chlorogenic acid was higher in the flowers of tolerant cultivars than in susceptible ones.
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The effect of rootstock on the nectar production of apple cultivar `Idared'
15-25.Views:137Approaches based on the daily rhythm of apple flowers provide a new stage in nectar research, where the synchronous functioning of sexual organs is studied. In the flower biological studies the insect attraction of flowers was also studied. The two most important factors of insect attraction are the pollen- and nectar production of the flowers. From 1993 to 1998 we studied the food, that flower had to offer for the pollinating insects at different times of the day.
Studies were carried out on 'Idared', one of the hybrids of 'Jonathan' apple cultivar. The fruit of 'Idared' is bigger than the fruit of `Jonathan'. It is bright red, transportable, has a bigger productivity and is not subject to Jonathan-spots. Concerning its inner characteristics, it is juicy, the flesh consistency is better than that of 'Jonathan', but its acid/sugar ratio is worse (Sansavini et al. 1981).
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Determination of auxine content of soft wood cutted `Marianna GF8/1' (Prunus cerasifera x P. munsoniana) by High Performance Liquid Chromatography during rooting period
67-70.Views:138The content of different auxins of soft-wood cutted plum rootstock 'Marianna GF8/1' (Prunus cerasifera x P. munsoniana) was determined during the rooting period. The level of auxin-concentration (exogenous and endogenous) of basic and intemodal part of cuttings was determined by WATERS HPLC equipment every 7 days during rooting period. The lengths soft wood cuttings were app. 30 cm long. The basal part of shoots were treated with 2000 pg/g concentrated indole-butiryc acid in talcum powder. After treatment the cuttings were placed in propagation green-house under intermittent mist. The plant hormones were extracted by methanol the solution was cleaned by paper-filter, and further cleaned by centrifuge. The effluent was examined by reversed phase High Performance Liquid Chromatography, with WATERS 2487 dual detector at 220 am on Symmetry C18 4,6x 150 column. Recovery and reproducibility assessment indicates good accuracy and acceptable relative standard deviation (RSD) 5%. Linear responses (r20.997) for calibration curve was obtained with IAA, IPA and IBA standard in range, with a limit of quantification of 0.15 g•m1-1. The concentration of IAA, IPA and IBA in the basal part of cuttings were measured, during the rooting period. We proved the external IBA was taken up by the plants. In the plants were found the IBA, and the IAA concentration of IBA treated cuttings was higher, than the untreated one.
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Peach latent mosaic viroid in naturally infected sweet cherry trees in southern Italy
37-38.Views:115Peach latent mosaic viroid (PLMVd) was found in naturally infected sweet cherry trees grown in commercial orchards in southern Italy. The viroid was detected in nucleic acid extracts of symptomless leaves by molecular hybridization with a PLMVd cRNA probe. The viroid was transmitted by grafting from sweet cherry to peach seedlings and identified in peach by molecular hybridization.
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Effect of seedling quality on growth, yield and quality of tomato (Solanum lycopersicum L.)
64-72.Views:311A two trial greenhouse experiment was carried out at Rwanda-Israel Horticulture Centre of Excellence located at Mulindi Station to evaluate seedling quality on growth, yield and quality of tomato. The seedlings were grown in different growing media and produced seedlings with varying quality indices. The growing media of peat moss 100% (T2) and sand + goat manure + carbonized rice husks 50%: 10%: 40% (T8) were revealed in seedlings with the highest mean quality indices of 31 and 28 respectively, while sand 100% (T2) presented the lowest quality indices during both trials. The transplants were planted in polybags filled with 2:1 of topsoil and kitchen manure arranged in a randomized complete block design (RCBD) with four replications. Collected data were subjected to analysis of variance and means were separated using HSD test at a 5% level of significance. The results revealed that the seedlings grown in T1 (S1) and T8 (S8) consistently presented tomatoes with better growth performance and yield. S1 and S8 produced mean yield of 93.59 and 92.35 t/ha respectively while S2 had the lowest yield with 53.86 t/ha. The fruit produced from seedlings grown in T4 (S4) had the highest mean sugar acid ratio of 5.88 but not significantly different from 5.61 and 5.44 of S1 and S8 respectively. Hence, there was a positive relationship among seedling quality and growth and yield performance of tomato but not in fruit quality.
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Comparative study of heirloom tomato varieties
31-33.Views:219The study examined the health status, growth type, berry morphology, berry quality, and gastronomical value of 60 heirloom tomato lines. In the middle of June, the level of virus infection was medium in the plant stock according to the assessments. 21% of the lines did not show symptoms of virus infection. During the vegetation period, the major fungal diseases were Alternaria by the middle of August and Phytophthora from the beginning of September. In spite of the fact that no fungicides were applied, 13% of the lines were healthy and 36% showed medium infection at the end of August. Half of the lines had a strong, 23% of the lines had a medium growth vigour. The majority (74%) of the lines showed strong or medium lateral shoot growth and only 7% of them proved to be less prone to branching. In spite of the rapid growth, 20% of the lines had a weak main stem, the majority of the lines had a main stem medium strength. The different shapes occurred in different colours, in addition to the traditional red colour (53%), there were orange, yellow, wine-red (blackish), lilac, green, almost white or multicoloured varieties. Unfortunately, most of the lines were prone to longitudinal or circular cracking of the berries. Based on the first experiences, all tested variety types had more favourable utilization characteristics then the traditional tomatoes. Varieties with a thick flesh and harmonic acid/sugar ratio such as some of the ox-heart varieties are especially valuable. For drying, the elongated types proved to be the best. The hollow tomato is the most special type which can be utilized most diversely. It is especially suitable for making white tomato soup and, “Concasse" due to its easily removable placenta rich in jelly materials and its thick flesh. In addition, it can be used for preparing stuffed tomato.
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Studies on the alkaloid production of genetically transformed and non-transformed cultures of Lobelia inflata L.
65-71.Views:152The investigations of the growth and alkaloid production of cell suspension-, callus-, organized- and hairy root cultures from Lobelia inflata L. proved that these cultures are able to synthesize the characteristic piperidine alkaloids of the intact plant. Alkaloid precursor amino acids (Phe, Lys) and plant growth regulators affect not only the growth and differentiation of tissue cultures but also their secondary metabolism. The synthetic regulator Sz/I I combined with Phe increased the total alkaloid content considerably in callus- and organized cultures; regulator Sz/28 especially increased the lobeline content (in organized cultures in response to Lys, in callus tissues as a result of Phe application). With the aim of optimizing growth and alkaloid production of the genetically transformed hairy root cultures of Lobelia inflata L. we studied the effect of some growth regulators (NAA, IAA, kinetin) and precursor amino acids (Lys, Phe). The kinetin had inhibiting effect on the growth and lobeline production of the hairy roots. The IAA and NAA increased the biomass formation and lobeline production. The highest lobeline level was detected in tissues cultivated on hormone-free medium containing Phe.
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Historical apple cultivars that display high level of resistance to fire blight
19-23Views:142Following the first outbreak of fire blight caused by the bacterium Erwinia amylovora (Burrill) Winslow et al. in Hungary, we have started research with the aim to screen domestic gene sources, mostly historical Hungarian apple cultivars, for disease resistance as part of an apple breeding program for multiple resistance. The present study was conducted with the aim to choose the most tolerant historical apple cultivars among 25 selected cultivars by screening their behavior towards fire blight under controlled conditions. Six cultivars were shown to be the most disease tolerant: `Batul', 'London pepin', Nemes Sóvári Alma', `Sikulai', `Szemes alma', Wilmos renet'. We evaluated these cultivars by investigating both morphological- characteristics under original environmental circumstances and fruit quality. The cultivars had a remarkable degree of fire blight resistance compared to the control cultivars. These were not competitive with the commercially grown 'Jonathan M40' during cultivar tests but on the basis of certain characteristics they could serve as genetic sources for breeding new varieties.
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Ultrastructural and biochemical aspects of normal and hyperhydric eucalypt
61-69.Views:251Hyperhydricity was observed throughout in vitro multiplication phase of a Eucalyptus grandis clone. Ultrastructural approach of tissue and cell differentiation, izoenzyme patterns, binding protein (BiP) expression, and pigment content were performed. Hyperhydric tissues showed a reduction in cell wall deposition, reduction of membranous organelles, higher cell vacuolation, and more intercellular spaces than its normal counterpart. Additionally, several vesicles were present in hyperhydric cells suggesting the occurrence of organelle autophagy by autophagic vacuole. Lower pigment content, intercellular spaces on the epidermis and the induction of a molecular chaperone (BiP) were observed in hyperhydric phenotype. Evidences of schizolysigenous process of intercellular space formation are compatible with a stress condition. Although plastoglobulli were observed in normal and hyperhydric chloroplasts, they were more evident in the normal ones. Abnormal stomata also reflected a disruptive situation and morphogenesis disturbances which would difficult plant acclimatization. Further observation of the epidermis ultrastructure allows us to conclude that the presence of intercellular spaces on its surface may be constraining the recovery and development of hyperhydric plants. Similarly to BiP, other proteins such as esterase (EST), acid phosphatase (ACP), malate dehydrogenase (MDH) and peroxidase (PDX) are possible to be used as stress markers in in vitro conditions. Our results confirm earlier findings about negative effects of hyperhydricity on in vitro plant morphogenesis and ultrastructure, which in eucalypt is associated with a stressful condition contributing to lower propagation ratios.
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Effects of indole-3-butyric acid levels and activated charcoal on rooting of in vitro shoots of apple rootstocks
25-28.Views:314Rooting responses of rootstocks cvs. JTE-F1, M. 26 and MM. 106 were studied to different concentration of IBA in root induction media and to presence of activated charcoal in root elongation media. High rooting rate (>90%) could be achieved in cvs. JTE-H and M. 26, while cv. MM. 106 showed weak rooting ability at each IBA level tested. Increasing IBA content depressed the rooting only in cv. M. 26. Presence of activated charcoal decreased considerable the rooting rate in cv. M. 26 and decreased the number of roots in cvs. JTE-H and M. 26. These cultivars developed longer roots on media containing activated charcoal, while cv. MM. 106 did not showed any reaction for it.