Articles

Vegetative and micropropagation potential of Piper guineense (Schumach and Thonn)

Published:
2023-07-20
Authors
View
Keywords
License

Copyright (c) 2023 International Journal of Horticultural Science

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

How To Cite
Selected Style: APA
SAKPERE, A. M., & Ezenu, V. N. (2023). Vegetative and micropropagation potential of Piper guineense (Schumach and Thonn). International Journal of Horticultural Science, 29(1), 29-36. https://doi.org/10.31421/ijhs/29/2023/12554
Abstract

The continuous loss of forest plants due to deforestation, and the increasing demand for Piper guineense because of its medicinal and food value, has put a permanent pressure on its population in the wild where it is collected. A method for conservation and mass propagation is therefore required. This research was undertaken to determine the optimal concentration of auxin needed for vegetative propagation and to investigate the potential of Piper guineense for micropropagation. The auxin optimization study of vegetative propagation was based on the use of two-nodal stem cuttings treated with five different concentrations of indole-butyric acid (IBA). Growth parameters such as the number of sprouted, rooted and survived cuttings among others were determined. To investigate the potential of Piper guineense for micropropagation, nodal explants were subjected to different sterilizing treatments using ethanol, NaOCl, mancozeb, streptomycin and Plant Preservative Mixture (PPM). The effect of plant growth regulators (PGRs) was tested on sterilized nodal explants using full strength Murashige and Skoog (MS) hormone-free media alone as control and MS media supplemented with PGRs (BA, NAA and KIN) at different concentrations and combinations. Significant differences were observed across the treatments for all growth parameters measured. However, 2000 ppm IBA significantly (p<0.05) influenced sprouting and rooting of the stem cuttings. Piper guineense explants have deep tissue contaminants, which cannot be eradicated by surface sterilization alone except double sterilization using PPM. On control media, neither shoot nor root response was observed while the highest percentage of induced roots was obtained from explants cultured on MS +1 mg/L BA + 0.25 mg/L NAA. Shoot induction was only achieved when BA was used alone and when subcultured on media supplemented with NAA, which generated roots.

References
  1. Abshahi, M., García-Morote, F.A., Zarei, H., Zahedi, B., Nejad, A.R. (2022): Improvement of Rooting Performance in Stem Cuttings of Savin Juniper (Juniperus sabina L.) as a Function of IBA Pretreatment, Substrate, and Season. Forests 2022, 13: 1705. https://doi.org/10.3390/f13101705
  2. Afroz, T. (2020): Micropropagation of Black Pepper (Piper nigrum L.). Dept. of Biotechnology, Faculty of Agriculture, Sher-e-Bangla Agricultural University, Dhaka. MSc Thesis. http://archive.saulibrary.edu.bd:8080/xmlui/bitstream/handle/123456789/3587/14-06113.pdf?sequence=1&isAllowed=y Accessed 22/03/2023
  3. Alaje, V. I., Amadi, J. O., Williams, A. O., Oyedji, O. F., Geply, O. A., Adebusuyi, G. A. (2022): Vegetative propagation of African black pepper (Piper guineense): The role of growth hormones and rooting media. Australian Journal of Science and Technology, 6(2): 71 – 77. https://www.aujst.com/vol-6-2/02_AJST_2022-15.pdf
  4. Anny, J. (2011): In vitro Regeneration of black pepper (Piper nigrum L.), Faculty of Resources Sciences and Technology, Universiti Malaysia sarawak. UNIMAS Institutional Repository. MSc Thesis.
  5. Chawla, H. S. (2000): Introduction to Plant Biotechnology. Inc. Enfield, New Hampshire, USA, Science Publisher, 2: 230-242.
  6. Clair, A.W., Etukudo, O. J. (2000): Food Security and Nigeria Agriculture; A paper presented in Food Security Conference in Lokoja. Nigeria
  7. Da Silva, B., Jonny, E., Scherwinski, P. (2012): A rapid in vitro protocol for propagation of Piper aduncum and Piper hispidinervum, two species from Amazon region with multipurpose uses. African Journal of Biotechnology, 11: 39-46. DOI: 10.5897/AJB12.1888
  8. Ferriani, A. P., Deschamps, C., Amaral W. D., Da Silva, L. E. (2018a): Propagation of three native Brazilian Piper species by cuttings and indole butyric acid. Revista Colombiana De Ciencias Hortícolas 12 (2): 491-499. Doi: http://doi.org/10.17584/rcch.2018v12i2.7359
  9. Ferriani, A. P., Gomes, E. N., Krinsk, D., Deschamps, C. (2018b): Vegetative propagation of Piper aduncum L. (matico) using cuttings of varying lengths and different substrates. Revista Cubana De Plantas Medicinales 23(3): https://revplantasmedicinales.sld.cu/index.php/pla/article/view/645/318
  10. Gostin, I. (2008): Effect of different plant hormones on Salvia officinalis cultivated in vitro. International Journal of Botany, 4:430-436. DOI: 10.3923/ijb.2008.430.436 URL: https://scialert.net/abstract/?doi=ijb.2008.430.436
  11. Kane, M. (2003): Bacterial and fungal indexing of tissue culture. Plant Cell, Tissue and Organ Culture, 6 (2):21-27.
  12. Khan, S., Banu, T. A., Islam, M., Habib, A., Ferdousi, A. Das, N., Akter, S. (2017): In vitro Regeneration of Piper nigrum L. Bangladesh Journal of Botany 4(2): 789-793. https://www.bdbotsociety.org/public/article/2017%20June/S-02.pdf
  13. Khan, S., Goswami, B., Akter, S., Islam, M., Noon, A. H., Habib, A., Banu, T. A. (2019): In vitro mass propagation of Piper betle L. Bangladesh Journal of Botany, 48(3), 559–566. https://doi.org/10.3329/bjb.v48i3.47917
  14. Kulkarni, A. A., Krishnamurthy, K. V. (2002): Culture medium composition useful for induction and proliferation of Taxus calli. US Patent, 6: 365-407.
  15. Morufu, E., Balogun, E. E., Besong, S. F., Adjobissie, O. S., Mbamalu, (2016): A review of Piper guineense (African Black Pepper). International Journal of Pharmacy and Pharmaceutical Research, 6 (1): 368-384. https://ijppr.humanjournals.com/wp-content/uploads/2016/05/34.Elizabeth-E.-Besong-Morufu-E.-Balogun-Serges-F.-A.-Djobissie-Ogochukwu-S.-Mbamalu-Jacinta-N.Obimma.pdf
  16. Nwachukwu, C. U., Ume, N. C., Obasi, M. N., Nzewuihe, G. U., Onyirioha, C. (2010): The qualitative uses of some medicinal plants in Ikeduru LGA of Imo state, Nigeria. New York Science Journal, 3 (11): 129-134.
  17. Okafor, J.C. (1997): Conservation and Use of Traditional Vegetable from Woody Forest Species in Southeastern Nigeria. In Promoting the Conservation and Use of Underutilized and Neglected Crops. Proceedings of the IPGRI International Workshop on Genetic Resources of Traditional Vegetables in Africa. Conservation and Use, Nairobi, 29-31 August 1995.
  18. Okafor, O. T., Uzochukwu, S. V. A. (2015): Establishing a Method for The Mass Propagation of Piper guineense (Schumach) via Tissue Culture. MSc Thesis. Retrieved from http://repository.fuoye.edu.ng/handle/123456789/1347
  19. Okigbo, K. N., Igwe, D. I. (2007): Antimicrobial effects of Piper guineense “Uziza” and Phyllanthus amarus 'Ebe-benizo' on Candida albican and Streptococcus faecalis. Acta Microbiologica Hungarica, 54(4): 353-366. DOI: 10.1556/AMicr.54.2007.4.3
  20. Okoye, E. I., Ebeledike, A. O. (2013): Phytochemical constituents of Piper guineense (uziza) and their health implications on some microorganisms. Global Research Journal of Science 2 (2): 42-46
  21. Omamor, I.B., Asemota, A. O., Eke, C. R., Ezia, E. L. (2007): Fungal contaminants of the oil palm tissue culture in Nigerian Institute for Oil Palm Research (NIFOR). African Journal of Agricultural Research, 2 (10): 534-537. https://citeseerx.ist.psu.edu/document?repid=rep1&type=pdf&doi=c50455c663479ad90dd965bf9de4a8f64d61a593
  22. Pessarakli, M. (2002): Handbook of Plant and Crop Stress. CRC Press, Boca Raton, FL, 4:56-62.
  23. Philip, V., Joseph, D., Triggs, G. S., Dickinson, N. M. (1992): Micropropagation of black pepper (Piper nigrum Linn.) through shoot tip cultures. Leonard Hill (Books) Ltd., Eden Street, NWT, 2:137-139.
  24. Sameer, N. M., Nabeel, A, K. (2016): Effect of Different Sterilization Methods on Contamination and Viability of Nodal Segments of Cestrum nocturnum L. International Journal of Research Studies in Biosciences. 4(1): 4-9.
  25. Sundharai, K., Ponnuswami, V., Jasmine, J. (2002): Effect of Growth regulators in the propagation of hippli (Long pepper). South Indian Horticulture, 48:172-174.
  26. Udofia, S. I., Ekpa, N. E., Williams, D. E. (2016): Conservation status of Piper guineense Schum and Thonn. in home gardens of Uyo local government area, Akwa Ibom state, Nigeria. Journal of Forestry, Environment and Sustainable Development, 2(1):33-40.
  27. Udosen, I. R., Sam, S. M. (2015): Germination Studies and Early Seedling Growth of Some Local Spices Found in Niger Delta Nigeria. International Journal of Scientific Research 4 (6): 818- 819
  28. Waman, A.A., Bohra, P., Chakraborty, G. (2019): Vegetative Propagation of Piper sarmentosum Roxb.- A Medicinally Important Species. Current Agriculture Research Journal. 7(1):46-52
  29. Zhao. Y., Chen, Y., Jiang, C., Lu, M. Z., Zhang, J. (2022): Exogenous hormones supplementation improve adventitious root formation in woody plants. Frontiers in Bioengineering and Biotechnology 2022 Sep 13;10:1009531. doi: 10.3389/fbioe.2022.1009531. PMID: 36177185; PMCID: PMC9513251.