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  • Cooling irrigation as a powerful method for microclimate modification in apple plantation
    33-37.
    Views:
    1120

    Irrigation in some countries is a horticultural practice mainly used only to supply water. At the same time the use of microsprinklers have a powerful influence on the changes of temperature in orchards. When the air’s temperature is high (about 20 °C or higher) the evaporative cooling irrigation significantly decreases the plants’ surface temperature and air temperature. The cooling effect is stronger when the air is dryer. By using cooling irrigation regularly, canopy temperature can be decreased so that the beginning of blooming can be delayed. Also if the blooming is early and frost probability is high, serious damages can happen in orchards. The beneficial effect of cooling irrigation is the temperature reduction and frost protection. InMarch 2010, one month earlier than the expected blooming an irrigation system was established to produce anti-frost treatment and regulate the micro-climate of a Gala apple orchard which belongs to the University of Debrecen (Hungary). The objective of sprinklers was to cool the air by increasing water evaporation and relative humidity. The position of the micro-sprinklers was planned in three levels (around the tree trunks, a few cm near to the soil surface, in the crown region and above the crown, a half meter higher). The results showed that the water sprayed in the orchard by micro-jets influenced decisively the temperature of the plantation. At higher temperatures (around 20 °C), the drop of temperature may attain 5–7 °C. A low relative humidity of the air may increase the relative effect. When water was applied at intervals of 15 minutes for ten times a day from 8 am to 18 pm, the air, flowers and bud’s surface temperature could be kept low.At certain days when the temperature was higher than 10 °C, irrigation was used at night time in similar 15 minutes intervals, from 18 pm and 6 am. The beginning of bloom could be delayed for more than ten days. The Gala apple variety blooming dynamics was characterized by a logistic curve in the treated as well as in the control plot. In the treated plot, the curve was steeper than in the control one in spite of the equal temperatures measured in the plots. Under Hungarian climatic conditions, the method was successfully used to delay blooming dates. The main result was the diminution of the frost damage in the spring that assured apple yields.

  • Comparison of varying pollen source on productivity of sour cherry (Prunus cerasus L.) cultivars
    23-26.
    Views:
    1127

    Fruit set of five sour cherry cultivars (‘Újfehértói fürtös’, ‘Éva’, ‘Petri’, ‘Pándy 279‘ and ‘Csengôdi’) of eight years-old trees grown in Újfehértó, located in the Eastern north part of Hungary have been studied over two years (2008 & 2009). Following reciprocally cross-pollination, free-pollination, self-pollination (autogamy) artificial self-pollination (geitonogamy) were studied. The results show that both maternal and paternal parent cultivates had significant effects on the percentage fruit set. Significant differences have been found in fruit set among years and among pollination treatments. Fruit set of free-pollinated Pándy 276 cultivar was low and seasonally highly variable. The yield of this cultivar on self-pollinated flowers was nearly 0%, and in this treatment the maximum yields did not reach 10% in any of the examined cultivars. There was no significant relationship in the fruit set of free-pollination and natural self-pollination treatments.

  • Comparative study of three rosemary (Rosmarinus officinalis L.) clones during the growing season
    67-72.
    Views:
    549

    The growth rate of three rosemary clones, `Harmat', 'Salem' and `Horvát', their essential oil content and its components as well as the total phenol content and total antioxidant capacity in their aqueous and ethanolic extracts were compaired. Total phenol content (determined by Folin-Ciocalteau reagent) and antioxidant power (FRAP-value) of aqueous and ethanolic rosemary extracts were measured by spectrophotometric method. The essential oil content was determined from drug by water-steam distillation. `Harmat' showed the most intensive growth through the vegetation period. The total antioxidant power of the ethanolic extracts from all the three clones was similar: a decrease was observed at the end of the season. In most cases more antioxidant compounds were dissolved in the aqueous extracts. The total quantity of phenolic compounds shows a good correlation with the potential extent of stress effects. The difference among the phenol contents of the various clones can be derived from the difference in their tolerance, because phenols have antioxidant effects contributing to protection against harmful impacts. Difference between the two extraction methods (aqueous and ethanolic) is due to the distinct solubility of compounds. Some other components, like essential oil compounds also possess antioxidant effect and in this way they may influence the antioxidant power of extracts.

  • Agronomic and alimentary evaluation of elder (Sambucus nigra L.) genotypes selected from natural populations of Hungary
    49-52.
    Views:
    268

    The black elder (Sambucus nigra L.) is a native plant in Hungary represented by extended and very variable populations. Cultivation of elderberry started during the late 90-ies because of the growing interests of the processing industry. High anthocyanin content, nutritive value of the berries and aromatic compounds of the flowers have been the esteemed constituents.

    At the moment, there are about 3000 hectares elder plantations in Hungary, which is more than of any of the other European countries. Unfortunately, the cultures are planted to the Austrian selections of Haschberg, in spite of the fact that about 10-15 other selections of other foreign countries have been registered and planted elsewhere. In addition to that, the wild population of Hungary may offer a precious opportunity of further selection. Since 1970, Aladár Porpáczy at Fertőd and the Department of Fruit Growing of the former University of Horticulture in the 1980-ies started the selection in Hungary too.

    The present study deals with 7 genotypes, their morphology of berries and inflorescences. Results are concentrated on four genotypes: Szcs­1, Szcs-2, Szcs-3 and Szcs-5, which are all earlier ripening than the Haschberg varieties, and are superior in characters of fruit and inflorescence.

  • Agroclimatological properties of growing sites assigned to apple and pear production in Hungary
    95-97.
    Views:
    842

    Apple and pear growing sites in Hungary are classified into four regions according to the Hydro-thermic Coefficient: dry, moderately dry. moderately humid and humid. Most of the plantations of apple and pear are located in regions considered as moderately dry and moderately humid. Within that category, the two respective species have different preferences, i.e. the ecological features of Hungary give different opportunities for apple and pear growing. Apple is grown almost everywhere in the country, successfully. The selection of cultivar-regions is needed mainly for increasing competitiveness on the market. Main apple growing regions are listed in 3 large groups. For the definition of cultivar-regions, mainly the configurations of soil and precipitation, i.e. conditions of the soil and opportunities of gaining water were decisive. Market factors are also considered. The area assigned to pear is much less than that of apple, in Hungary. Some well known and popular varieties would require high air humidity which cannot be presented in most of Hungary. Therefore, the possibility to establish regions for pear varieties is restricted, we have to create a particular micro-environment. Two groups are potential. The first one comprises sites where the annual precipitation is 700 mm, at least. There, apple and pear production would compete each other. In more dry habitats (less than 700 mm annual precipitation), micro-environments should be found and only drought-resistant, mainly summer-ripe cultivars should be chosen with, preferably, low tendency of sclereid formation. In that case, neither irrigation could help to produce adequate quality in varieties sensitive to low air humidity.

     

  • Organic versus integrated apple growing: I. differences in soil and leaf parameters
    57-60.
    Views:
    1621

    The aim our study was to establish whether significant differences in nutrients uptake and quality of soil and leaf exist between organic and integrated grown apples. The study was performed at the orchard Fruit Research Station, University of Debrecen, at Debrecen-Pallag during 2002–2004. Macro and micro elements were measured in soil and plant samples. Analyses of variance of soil nitrogen data indicated highly significant differences between the two management systems (P < 0.001) for each examined nitrogen fraction. Analyses of variance of soil phosphate data indicated significant differences (P < 0.05) between the two management systems for orto-PO4 3– contents. Our data indicated that highly significant differences between the two management systems (P < 0.001) for magnesium, copper, and zinc; while significant differences between the two management systems was at P = 0.007 for calcium. Three year’s data of leaf phosphorus, sulphur and zinc were not shown significant differences between production systems. Nevertheless manganese and copper contents of leaves were higher in the organic orchard compared to the integrated one.

  • The investigation of suitability to various purposes of industrial processing in stone fruit varieties and variety candidates
    93-101.
    Views:
    1060

    In the laboratory of Conserve-technology in the Research Institute for Fruit Growing, Company of Public Utility, Cegléd, 6 sour cherry, 6 apricot, 5 peach and nectarine, 6 plum and 4 Japanese plum varieties (canned fruit, juice, dried fruit, deep frozen). The products were evaluated by organoleptic methods on a scale of 1-5 steps. The varieties receiving at least 4 points were listed (in brackets also the respective product was indicated): `Kántorjánosi' sour cherry (for all the three purposes), '13' variety candidate (canned and deep frozen), 'T' var. cand., (canned, deep frozen), 'Érdi bőtermő' (dried fruit), 'R' var. cand. (deep frozen); ‘Ceglédi arany', 'Ceglédi bíborkajszi', 'Magyar kajszi"C. 235' (fibrous juice); `Babygold 5', 'Redhaven' peaches, and 'Caldesi 2000' nectarine (canned); 'Stanley' plum (canned), 'Besztercei Bt, 2' (deep frozen).

  • Flower visiting activity of honeybees on fruit species blooming subsequently
    12-16.
    Views:
    1104

    In the small demonstration orchard of the College Faculty of Horticulture at Kecskemét the blooming time, the flower density and the honeybee activity was observed at a number of cultivars of 20 flower species during four consecutive years.

    Fruit crop species were in flower during 3-4 months altogether. The blooming period of them was classified into five groups as early (almond, apricot, gooseberry), middle early (sweet cherry, red currant, currant-gooseberry, black currant, white currant, peach, plum, sour cherry), middle late (pear, strawberry, apple), late (black elder, quince, medlar, raspberry, blackberry-raspberry) and very late blooming period (blackberry). The blooming period of the members of the groups of early and medium early blooming often coincided partly and the same happened between the medium and the medium late as well as between fruits of late and very late flowering.

    The flower density of some fruit species is extremely variable (currant-gooseberry, medlar), while at others it is fairly stable and evenly dense in consecutive years (sour cherry, sweet cherry, strawberry). At other fruit species it is moderately changeable. Some fruit species tended to attract more honeybees than others (plum, apple, quince, medlar) and some of them tended to attract much less (black elder, pear) but most species can be regarded as of medium attractivity.

    On the flowers of some fruit species (pear, strawberry, quince) honeybees gathered pollen predominantly. At most fruit species however pollen and nectar gathering behaviour seemed to be gradually changing during the season. Namely most honeybees tended to gather pollen at the flowers of the early blooming fruit species, but on the other hand typical foraging behaviour gradually shifted to nectar gathering at the flowers of fruit species of moderate and late blooming periods.

     

  • Spore dispersal, diurnal pattern and viability of Monilinia spp. conidia and the relationship with weather components in an organic apple orchard
    17-19.
    Views:
    628

    In a two-year Hungarian study, spore dispersal diurnal periodicity and viability of Monilinia spp. and their relation to weather components were determined in an organic apple orchard. Conidia of Monilinia spp. were first trapped in late May in both years. Low number of conidia were trapped until end-June. Thereafter, number of conidia continuously increased until harvest. Conidia in a 24-h period showed diurnal periodicity pattern, with th highest concentration in the afternoon hours. Spore viability with FDA staining showed that viability of
    conidia ranged from 45 to 70% with showing lower viability in the dry than in the wet days in both years. Temperature and relative humidity correlated positively with mean hourly conidia numbers in both years. Mean hourly rainfall was negatively but poorly correlated with conidiacatches in both years. Results were compared and discussed with previous observations.

  • Effect of foliar spraying with algae suspension on leaf and fruit quality parameters of apple varieties
    35-38.
    Views:
    1292

    Foliar nutrition experiment was made to investigate the effect of algae products on fruit quality of apples (Malus domestica Borkh.). The study was conducted in 2011 at Siófok inWest-Hungary on cv. ‘Jonagored’ and ‘Idared’grafted on M9 rootstock. In our trial leaf diagnostic and fruit quality measurements were made to study the effectiveness of applied products. Relative leaf chlorophyll content was determined by a portable chlorophyll meter. Fruit weight and shape index were measured. Fruit inner qualifying parameters like titratable acid content and Brix value were also determined. It can be stated that the used products had traceable effect on investigated fruit parameters. Although, leaf N content was not effected by treatments significantly, relative leaf chlorophyll content significantly increased by both treatments. Used products significantly increased the fruit weight and shape index. Applied treatments significantly decreased the pH of pulp. Treatments increased the acid contents (fumaric, citric, malic) of apples and decreased the amount of monosaccharides. It means that the applied treatments pushed out the ratio of acid/sugar.

  • In vitro comparative study of two Arundo donax L. ecotypes’ selenium tolerance
    119-122.
    Views:
    550

    Selenium tolerance of two somatic embryo-derived Arundo donax L. ecotypes (Blossom, 20SZ) were compared in in vitro culture. Sodium-selenate (1 – 100 mg L-1) as known the most phytoavailable selenium form and the less studied red elemental nanoselenium (100 mg L-1) were applied as selenium treatments. Basis on the results Blossom ecotype seemed to be more sensitive to the sodium-selenate than 20SZ. Inhibiting effect of selenate was effectuated above 10 mg L-1 in case of Blossom, which was manifested in decreased survival rate and growing parameters. Contrast to this 20SZ could tolerate the selenate ≤ 20 mg L-1 without any toxic symptoms. Lower selenate tolerance of Blossom could be explained with higher selenium accumulation. Both of two ecotypes could also uptake and accumulate the red elemental nanoselenium however in much less extent compared to selenate.

  • Spatial and temporal variation of extremely abundant maxima of precipitation in Hungary during the period between 1951 and 2010
    107-114.
    Views:
    20602

    The study deals with the accumulated database of 16 meteorological stations in Hungary during a period of 60 years. The purpose was to reveal the spatial and temporal structure of the appearance of extreme values in the daily distribution of data concerning precipitation. We strived to answer the question whether the frequency of incidences of daily maxima did they change or not during the 60 year-long period in the main growing regions of the country. It is demonstrated on geographical maps how the size and frequency of precipitation episodes ensued, and what are the typical traits of changes in intensity as well as in frequency of happenings projected according to their spatial and temporal distribution. From the point of view of fruit and vegetable growing, it is of prime interest what kind of frequency and intensity of changes occurred in precipitation. The temporal distribution of extremities though did not seem to change signifi cantly in some areas, but the recognition of changes may help conspicuously the planning and the choice between alternatives of species and varieties as well as technologies of horticultural managements for the long run. Extremely intense rains during a short time may cause erosion and stagnant water, thus we have to know what are the odds of risk. The temporary distribution of changes helps us to judge upon the reality of anxieties, which are expected according to the existence of trends. Seasonal or monthly distribution is visualised by maps, what is expected and what is accidental as for a decision in planning. The spatial distribution of coeffi cients of variation help us to decide what is the local chance of extreme happenings at different parts of the country and what is its coeffi cient of uncertainty. The risk of any undertaking dependent on conditions of weather could be expressed numerically by a coeffi cient of risk.

  • The tests of effectiveness of Frostbuster under excessive weather conditions in an apricot plantation
    87-91.
    Views:
    1194

    Frostbuster is a new system, engine and technology, developed to protect fruit plantations from the frost damage. In order to raise domestic experiences and measurements, experimental approach has been initiated to prove the utility of the system under excessively low temperature in the plantation of the Siófoki Gyümölcstermesztési Zrt (Fruit Growing Co. Siófok). The first opportunity ensued in the night of February 23-24, 2011, when the temperature sank to 12°C below zero. The question was to see whether we could prevent the drop of temperature by the frostbuster technique. The margin of an anticyclone staying on East Europe secured a stable condition to make tests. The only difference from the imaginable conditions of dangerous frosts was the heat keeping capacity of trees was weak, much inferior than compared with trees in full boom. As a consequence, the tree rows represented much lower heat-capacity and cooled down much quicker than blooming trees in springtime, i.e. their temperature was more variable. The other difference was, compared with an episode in spring that the hard frost lasted much longer than usually in spring. For testing the system, those conditions had even more advantage. Six meteorological stations helped us in measurement. Data-collectors were timed to 1 minute distances and the bulk of data proved to be beneficial for testing the Frostbuster. The results prove that the system is adequate to keep the temperature continuously higher than the surrounding field under excessively low temperatures. Further measurements are still needed to find the optimal solutions fitting to the growing site and its microclimate. Results presented offer a basis of further proofs.

  • Monitoring of water regime in an apple orchard
    29-32.
    Views:
    1030

    Our investigation was carried out at an micro-irrigated intensive apple orchard in Debrecen-Pallag in 2010. The aims of the study were to monitor the effect of a compacted layer on soil water regime by tensiometers and supporting the water management of the orchard. The results suggest that the physical characteristic of the examined soil is sandy soil with low capillarity and total available water content. The soil water tensions were varied between pF 0 and 2.5 due to the extreme precipitation circumstances in 2010. Tensiometers in 40 cm depth resulted fast (few hours) and significant respond to precipitation than in the 70 cm soil layer. Based on daily measurements, the soils possess a daily fluctuation of soil moisture, however the changes become more moderate in deeper layers. In accordance with all of the results, the amount of drainable water regime was about 20.6 V/V% at 40 cm depth and 18.6 V/V% at 70 cm mainly. The harmful surplus water can be infiltrated by loosening of the compacted soil layer in 50–70 cm depth or led off by vertical drainage.

  • Connection of fertilization conditions of sour cherry and meteorological parameters
    17-21.
    Views:
    17682

    Our analyses showed that the degree of free fertilization is mostly influenced by maximum temperature and sunshine duration.We found that free fertilization ratio increases with higher daily maximum temperatures; similar results characterise sunshine duration as well, namely we observed higher free fertilization ratio at higher sunshine duration values. Total amount of precipitation during the period between blossoming and maturity and the difference between the average daytime and night temperatures have an important role in the tendency of maturity time. Photosynthesis and respiration are essentially significant in the development of biological systems. These two processes are mostly regulated by the daytime and night temperatures. Therefore, it is not surprising that if the difference between daytime and night temperatures is large, it means intensive photosynthesis and a low degree of respiratory loss. Under these conditions intensive development and ripening can take place; however, in case of a low temperature difference intensive respiration slows down the process of development. Duration of ripening is also significantly influenced by the amount of precipitation of the period between blossoming and maturity.Abundant precipitation slows down the process of ripening, while dry weather accelerates it. Self-fertilization takes place in a space isolated from the environment. In spite of that, we found that effectiveness of self-pollination significantly depends on the meteorological conditions. Degree of self-fertilization is influenced directly by temperature and indirectly by other climatic parameters. We found a significant connection between the values of maximum and minimum temperatures during blossoming and the ratio of self-fertilization. Increase of maximum temperature reduces the effectiveness of self-pollination.A 1 °C increase of maximum temperature reduces self-fertilization ratio by 0,6%. In the case of minimum temperature we can state that the morning minimum temperature of 7,5–8,5°C is the most favourable. If minimum temperatures are under 4 °C or above 12 °C, self-fertilization ratio reduces to the quarter of the value characteristic at 8 °C. We believe that the effectiveness of self-fertilization can be improved considerably by the rational placement of isolator bags within the crown area, avoiding their placement to the external, western crown surface.

  • Hazardous element content and consumption risk of 9 apricot cultivars
    61-65.
    Views:
    951

    The heavy metals pollution is one of the problems that arise due to the increased uses of fertilizers and other chemicals to meet the higher demands of food production for human consumption. In order to assess possible health risk of apricot (Prunus armeniaca L.) consumption, levels of Arsenic, Cadmium, Mercury and Lead were determined in fresh and dried samples of "Jumbo cot", "Tom cot", "Gold strike", "Gold bar", "Bergeron", "Bergarouge", "Sweet cot", "Yellow cot" and "Zebra" apricot cultivars. Wet digestion of samples with concentrate HNO3 – H2O2 digester mixture and inductively coupled plasma–atomic emission spectroscopy was used. Highest content of As, Cd, Hg and Pb among all cultivars, were 0.5, 0.04, 1.5 and 0.5mg/kg of dried apricot samples. Fresh fruit samples also contain 0.2, 0.016, 0.6 and 0.2 mg/kg of Arsenic, Cadmium, Mercury and Lead respectively. Daily intake of metals, hazard quotient and health risk index to reveal health risk possibility of dried and fresh fruits consumption were calculate and compared.

  • Model experiments for establishment of in vitro culture by micrografting in apple
    47-49.
    Views:
    470

    Micrografting was used in our experiments for establishment of in vitro culture from one rootstock (`JTE-F') and three scion cultivars (`Remo', 'Rewena' and `Reanda') of apple. Shoot tips of these cultivars were harvested from field and grafted onto in vitro rootstock cultivars. Their survival and development were studied. 42-93% of shoot tips survived and developed further depending on cultivar. Impermanent browning of sticking agar-agar could be observed in 21-25% of the micrografts depending on cultivars but discolouration of agar-agar ceased within one week and did not cause any death of shoot tips. We used micrografting successfully for establishment of in vitro culture from cultivars, from which earlier with conventional methods the culture establishment was not possible because of hard tissue browning. However, further studies are necessary to ensure the survival and development of shoots after removing them from micrografts.

  • Adaption of temperate climate horticultural plants in tropical and subtropical developing countries (Review Article)
    7-11.
    Views:
    714

    Adaption of temperate climate horticultural plants in tropical and subtropical developing countries (Review Article)

  • Investigation of flowering dynamics of the basil (Ocimum basilicum L.) and its consequences in production
    74-80.
    Views:
    470

    The flowering dynamics of Ocimum basilicum L., (a common population maintained at the UHFI) was studied with the aim to create an exact and practically applicable method for definition of phenological phases.

    According to our observation the development of individual flowers of the basil can be characterized by 8 distinguishable phases, which must be considered for description of the actual phenological stage of a spike.

    An accurate model was created for the unambiguous description of flowering process of different flowers within the spike and for the individual plant as a whole. The new flowering index formula is calculated from the number of flowers weighed by their phenological phases.

    The time dependence of flowering is presented by functions fitted to values of the flowering index. The results reflected different patterns of the main inflorescence, the inflorescences formed on the side shoots in the first or in the second half of the flowering period. However, for description of the flowering process of the whole plant, a sigmoid function proved to be the appropriate model.

    The accumulation process of the essential oil could be characterized by the flowering index values. They showed close correlation (r=0.964) at high probability.

    The new method assures an exact definition of phenological phases in basil. Its application seems to be optimal during breeding procedures, seed production and even in production of high quality drugs, according to the requirements of the Good Agricultural Practice (Guidelines for GAP, 1998).

     

  • Changes of the characteristics of Satureja hortensis L. herb during flowering period
    56-60.
    Views:
    264

    In this study the changes of the characteristics of Satureja hortensis herb were investigated during flowering period, in case of savories of 4 different origins. The change of drying ratio, leaf/stem ratio, essential oil content, the ratio of several essential oil components (carvacrol, y-terpinene, p-cymol, a-pinene, p-pinene and fi-caryophyllene) were studied from bud formation till seed ripening.

  • Specialities of the vegetation start and level of primary fruit set affect fruit quality
    17-22.
    Views:
    596

    Thinning is a h ighly crucial point of the apple production technology. According to results of numerous studies the earliest thinning is deemed to have the best amending effects. There can be considerable difference between trees of the same cul tivar and age in a plantation in respect to their flowering, in the numbers of fruits set and also in their canopy volume. Thus it can be crucial -just l ike in the case of pruning- to establish a fruit thinni ng su i ted for specif ic characteristics of actual trees. This experiment was established to examine how does primary fruit set (fruit load before thinning) and further on specific (fruit/TCS cm2) and absolute (fruit/tree) fruit-load of the trees affect quality attri butes at harvest. Our experiment was establ ished in a plantation on medium-tight loamy soil in north-east of Hungary. Harvest date was determined w ith the joint observation of the calendar date, starch-index, flesh firmness, background colour and fruit weight. Three fruit-load levels were established based on local experience and on data of several years, I Ot/ha lower and higher besides the advised optimum yield in the same orchard with slender-spindle shaped 'Gala must' cultivar standing on M9 rootstock in 1m x 3,8 m spacing. In the establishment of the 15, 25 and 35t/ha fruit-load levels on 20-20 apple trees total number of fruits set was counted at each tree. After this number of apples due to be removed was defined using an objective index on the basis of trunk cross sections (fruit/TCScm2) (Lafer, 1999). The following attributes were measured: weight (g), flesh firmness (lb/cm2) total soluble solid content (Brix %) and total titrate-able acid content (g/J).From the data on sugar and acid content quality index (Pomona value) was determined (Thialult, 1970). We could ascertain, that in an orchard, of the same aged but in concern to trunk cross sections somewhat different trees besides the specific index (apple/TCS cm2 the absolute fruit load (fruit/tree) can also be an important data, that has considerable effect on the internal quality. Secondly we could observe, that higher level of fruit load before thinning (primary fruit set) negatively affects quality index of the apples irrespective of the specific fruit load level (fruit/cm2 TCS) set later. Results underlines necessity of the earliest chemical thinn ing.

  • The Effects of Some Parameters on Agrobacterium-Mediated Transformation in Muskmelon
    46-49.
    Views:
    371

    Some parameters involved in Agrobacterium-mediated transformation in muskmelon Hales best (HBS) were studied. Cotyledon explants excised from 3.5-day-old seedlings were co-cultivated with Agrobacterium tumefaciens harbouring binary vectors which contained GUS and BAR genes. After co-cultivation on a low pH medium, explants were transferred to selective medium, with higher pH, containing Claforan and Finale. The medium was changed every two weeks till shoots were induced. All shoots rooted on MS medium supplemented with 0.3 mg/L IBA. These parameters combined as a whole led to successful transformation. The expression of the introduced gene construct was confirmed by GUS staining of shoot segments.

     

  • The Hungarian peach production risks
    85-89.
    Views:
    319

    Hungary possesses very good agroecological conditions even in a European comparison. Agriculture and food production is highly important despite its decreasing relative economic significance, since providing high quality and affordable food for the population is a basic social necessity and one of the decisive factors in quality of life. The development of vegetable and fruit production is of great importance both from a healthy nutrition as well as from a comparative advantage (cheap labour, expertise, production traditions, favourable climate) point of view.Vegetable and fruit consumption per capita is lower than in developed countries, which is a decisive indicator of healthy nutrition, but improvement is continuous and desired. Appropriate quality and quantity in production is a requirement to meet customers’ needs in a highly competitive market from one year to the next. Hungary can only be competitive if production results are improved while risks are reduced. Fruit production is a sector with high capital requirements, risk assessment is very important from a production, investment as well as from a national economy point of view. Our examinations will focus on production risk, which is present in the annual fluctuation of average yields, especially as a result of extreme weather factors.

  • Susceptibility of sour cherry cultivars to isolates of Monilia laxa (Ehrenbergh) Saccardo et Voglino
    83-87.
    Views:
    496

    In this study, the susceptibility of 7 commercially important sour cherry cultivars to Monilinia laxa was studied. Artificial inoculation was made with M. laxa isolates, which were isolated from different woody plants. Artificial inoculation was prepared in the laboratory and in the field. In laboratory, flowers of sour cherries while in the field, the two-year old twigs were inoculated in 2006 and 2007. According to results of stigmata inoculation, there were infection ability differences among the isolates originated from five different stone fruit host. Cultivars could be sorted into two susceptibility groups. In the field, twig inoculation in 2007 was made at blossom period and in 2007 at harvest. Seven sour cherry cultivars were inoculated with 8-day-old mycelial culture of M. laxa originated from sour cherry and almond. The agressivity and pathogenicity of the two isolates were measured by the degree of floem death: Results showed that year and phenological stage considerably influenced the degree of symptoms caused by the fungus. After artificial inoculation, tissue death progression was studied by fluorescent microscope. According to results, sour cherry cultivars were sorted into disease susceptibility groups. Susceptibility orders were identical to results on stigmata inoculation.

  • New in vitro micrografting method for apple by sticking
    79-83.
    Views:
    529

    The requirements for in vitro micrografting in apple are described. In vitro multiplicated shoots of cv. Royal Gala were the sources of rootstocks and scions after different pre-treatment, respectively. Oxidative browning of cut surfaces could be inhibited by the use of antioxidant mixture during grafting process. Scion base cut in v-shape was stuck by 1% agar-agar solution into the vertical slit of rootstock. There was no any displacement and the rate of fused and further developed grafts was 95 percent. Agar-agar between the rootstock and scion made the transport of different materials possible and hold the graft units together until the fusion took place. Fusion was proved also by histological studies. Some of in vitro micrografts were planted and acclimatisated and the survival was 100 percent.

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