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Studies on the alkaloid production of genetically transformed and non-transformed cultures of Lobelia inflata L.
65-71.Views:134The investigations of the growth and alkaloid production of cell suspension-, callus-, organized- and hairy root cultures from Lobelia inflata L. proved that these cultures are able to synthesize the characteristic piperidine alkaloids of the intact plant. Alkaloid precursor amino acids (Phe, Lys) and plant growth regulators affect not only the growth and differentiation of tissue cultures but also their secondary metabolism. The synthetic regulator Sz/I I combined with Phe increased the total alkaloid content considerably in callus- and organized cultures; regulator Sz/28 especially increased the lobeline content (in organized cultures in response to Lys, in callus tissues as a result of Phe application). With the aim of optimizing growth and alkaloid production of the genetically transformed hairy root cultures of Lobelia inflata L. we studied the effect of some growth regulators (NAA, IAA, kinetin) and precursor amino acids (Lys, Phe). The kinetin had inhibiting effect on the growth and lobeline production of the hairy roots. The IAA and NAA increased the biomass formation and lobeline production. The highest lobeline level was detected in tissues cultivated on hormone-free medium containing Phe.
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New trends in selection of poppy (Papaver somniferum L.)
69-75.Views:270Since the isolation of morphine by Sertümer in 1805 more than 40 alkaloids have been isolated from the poppy (Papaver somniferum L.). Some of them have high biological-pharmacological activity and economical importance, while others have none, or restricted ones. The increasing demand for poppy alkaloids is the consequence of the widening of the medical application of morphine and its related compounds: the quantity of morphine used for the treatment of pain reached a record level of 17.9 tonnes in 1997, compared with an annual average of 2.2 tonnes used during the period 1978-1983. However, the production of raw material (either opium, or dried capsule is produced) has to be re-evaluated taking into consideration the UN Convention signed in 1988 against Illicit Traffic in Narcotic Drugs and Psychotropic Substances. The countries were forced by the Convention to introduce new arrangements in poppy production including selection and introduction of new cultivars.
In the present work up to date results of poppy selection are reviewed explaining the biosynthetic and eco-physiological background of their alkaloid accumulation. The effectiveness and the possibilities of traditional selection methods as well as the probability of the application of biotransformation for producing cultivars accumulating low or high alkaloid content or plant material with special alkaloid spectrum (codeine, thebaine, narcotine) are discussed. The examples of Hungarian cultivars 'Monaco', 'Kék Gemona' and 'Tebona' are given in more detail.
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Leaf number and plant height of three Canna x generalis cultivars
22-24.Views:244The aim of this three-year study was to evaluate the effect of three treatments (thiophanate methyl, charcoal dust, and control) on leaf number and plant height on three Canna x generalis cultivars (City of Portland, Richard Wallace and Robert Kemp). Results showed that the 3-year means of leaf number ranged from 13.9 to 23.1 for the three cultivars. The largest leaf numbers were for cultivar Robert Camp (23.1 in 2010) and the lowest for cultivar City of Portland (13.9 in 2011). Thiophanate methyl treatment produced the highest number of leaves and the values were significantly different at P< 0.05 from the control for all years and for all cultivars. Numbers of leaves in charcoal dust treatments were larger than the control treatments but it was not significantly different. Results on plant height ranged from 69.2 to 160.5 cm for the three cultivars. The largest plant height was for cultivar Robert Kemp (160.5 cm in 2011) and the lowest for cultivar City of Portland (69.2 cm in 2011). Thiophanate methyl treatment produced the highest plant height and the values were significantly different at P< 0.05 from the control for all years and for all cultivars. Plant heights in charcoal dust treatments were larger than the control treatments but it was not significantly different.
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Cooling irrigation as a powerful method for microclimate modification in apple plantation
33-37.Views:245Irrigation in some countries is a horticultural practice mainly used only to supply water. At the same time the use of microsprinklers have a powerful influence on the changes of temperature in orchards. When the air’s temperature is high (about 20 °C or higher) the evaporative cooling irrigation significantly decreases the plants’ surface temperature and air temperature. The cooling effect is stronger when the air is dryer. By using cooling irrigation regularly, canopy temperature can be decreased so that the beginning of blooming can be delayed. Also if the blooming is early and frost probability is high, serious damages can happen in orchards. The beneficial effect of cooling irrigation is the temperature reduction and frost protection. InMarch 2010, one month earlier than the expected blooming an irrigation system was established to produce anti-frost treatment and regulate the micro-climate of a Gala apple orchard which belongs to the University of Debrecen (Hungary). The objective of sprinklers was to cool the air by increasing water evaporation and relative humidity. The position of the micro-sprinklers was planned in three levels (around the tree trunks, a few cm near to the soil surface, in the crown region and above the crown, a half meter higher). The results showed that the water sprayed in the orchard by micro-jets influenced decisively the temperature of the plantation. At higher temperatures (around 20 °C), the drop of temperature may attain 5–7 °C. A low relative humidity of the air may increase the relative effect. When water was applied at intervals of 15 minutes for ten times a day from 8 am to 18 pm, the air, flowers and bud’s surface temperature could be kept low.At certain days when the temperature was higher than 10 °C, irrigation was used at night time in similar 15 minutes intervals, from 18 pm and 6 am. The beginning of bloom could be delayed for more than ten days. The Gala apple variety blooming dynamics was characterized by a logistic curve in the treated as well as in the control plot. In the treated plot, the curve was steeper than in the control one in spite of the equal temperatures measured in the plots. Under Hungarian climatic conditions, the method was successfully used to delay blooming dates. The main result was the diminution of the frost damage in the spring that assured apple yields.
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Cracking susceptibility of sour cherry (Prunus cerasus L.) in Hungary and relation to calcium application
109-118.Views:281The rain induced fruit cracking is a big, serious problem especially for sour cherry growers but in some year sour cherry growers had also problem with fruit cracking caused by too much rainfall in the harvesting season. The cracked sour cherry fruits can be easily infected by different diseases like Monillinia sp. Cracked and infected fruits can not be transported for long distance and using for preservation because they lost their market value by the pour fruit quality. There are two possibilities to protect fruits against the rain induced fruit cracking. The most effective protection technique is the plastic rain cover over the tree rows. The installation of these equipments is too expensive for the growers. That is the reason why researchers tried to find other less expensive and sufficiently effective ways like sprayings different mineral salts, hormone and other type chemicals against the rain induced fruit cracking. Several calcium formulas calcium chloride (CaC12), calcium hydroxide (Ca (OH),) and calcium nitrate (Ca(NO3)2) can be sprayed in appropriate solution concentration.
The aim of our trial is to determinate the fruit cracking susceptibility of wide grown Hungarian sour cherry varieties and find the most effective calcium formula and its concentration for spraying in orchards to prevent the fruit cracking. In the first trial year (2006) cracking index of tested sour cherry varieties were determined under field and laboratory conditions. Under field conditions were not found differences between cracking tendency of tested cultivars. After results of immersing fruits in distillated water for 24 hours tested sour cherry varieties were divided to three groups by the susceptibility to rain induced fruit cracking: very susceptible (`Maliga emléke', 'Piramis', 'Érdi jubileum', 'Erdi nagygyümölcsű’ and 'Meteor korai'); susceptible ‘Érdi bőtermő, Tandy 279' and Cigány 59.; moderately susceptible/tolerant ("T" and "R" clones). In the second trial year (2007) calcium chloride (CaCl2) and calcium nitrate (Ca(NO3)2) were sprayed in 0,35m/m%; 0,5m/m% and 1,0m/m% solution concentration. One more commercial product "Damisol-Kalcium" was also applied in the advised 1,0m/m% concentration. Like in the trial year before (2006) under field conditions we did not kept differences between the cracking susceptibility of varieties and calcium treatments. As the result of laboratory testing (immersing calcium treated fruits in distillated water) we kept that calcium chloride (CaCl2) seems the most effective against the fruit cracking in 0,5m/in% solution concentration. The other calcium formulas also decreased the cracking ratio but in less scale.
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Total anthocyanine content and antioxidant density of some Hungarian sour cherry varieties
109-111.Views:226Evidence suggests that a diet high in fruits and vegetables may decrease the risk of chronic diseases, such as cardiovascular disease and cancer, and phytochemicals including phenolics, flavonoids and carotenoids from fruits and vegetables may play a key role in reducing chronic disease risk. Recent research has proven that sour cherry (Prunus cerasus L.) is a valuable natural source of some bioactive compounds important in human health preservation. In our work, we identified the total antioxidant activity, anthocyanine content and "antioxidant density" of sour cherry varieties named "Újfehértói fürtös", "Debreceni bőtermő", "Kántorjánosi" and "Érdi bőtermő" and those of the "Bosnyák" sour cherry clones. "Antioxidant density" is a biological value indicator obtained in a synthetic way, which indicates the antioxidant capacity of the particular food, e. g. fruit and vegetable, related to 1 Calorie.
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The effect of the limitation of insect pollination period on the fruit set and yield of quince cultivars (Cydonia oblonga Mill.)
103-108.Views:186The effect of the partial and/or complete limitation of the insect (bee) pollination period was studied in three consecutive years at 6 quince cultivars. Quince is greatly sensitive to the limitation. Complete limitation of insect pollination resulted in no yield and also a partial limitation of the insect pollination period (exclusion of the bees at the first or at the second half of the blooming) depressed the final set and the yield by 60-70% at least or more, sometimes down to no yield. No real correlation was found between the mean mass of fruits and the fruit set. The mass of fruit seems to be rather a character of the cultivars. A loose but significant correlation was established between the intensity of honeybee visitation and the consequent yield of quince. As much as some 4-5 and 8-10 honeybee visits are needed a day on one flower to achieve the required optimal fruit set that has been declared to be 20-25% for quince in the literature. These are extremely high bee visitation figures, accordingly, no doubt the commercial quince plantations require much higher number of honeybee colonies than other temperate zone fruit tree species to supplementary pollination
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Morphological, physiological features and differences of Vriesea splendens ’Fire’ plants during in vitro multiplication and rooting
Views:114During in vitro multiplication and rooting of Vriesea splendens ’Fire’, 0.1, 0.2, 0.4 and 0.8 mg l-1 benzyladenine (BAP), benzyladenine-riboside (BAPR), kinetin (KIN), meta-topoline (MT), indole-butyric acid (IBA) and naphthalene-acetic acid (NAA) were added to basal Murashige and Skoog (1962) MS medium. As compared to the hormone-free control, plants developed significantly more shoots on medium supplemented with almost all cytokinins (excepting KIN), especially BAP resulted the highest multiplication up to almost 26 shoots. Enhancement of cytokinin concentrations increased shoot number (and in case of BAP, peroxidase activity) but decreased plant height and rooting parameters. Regarding root production, both auxins were definitely beneficial (0.2 mg l-1 NAA resulted more than 7.5 roots and higher auxin concentrations efficiently stimulate root elongation); however, KIN had similar effects. After a three-month duration time of acclimatization, we observed that plants which were previously cultured on medium containing certain cytokinins (KIN in all doses and 0.1 mg l-1 MT) or both auxins had greater survival, moreover, as negative after-effect, higher cytokinin concentrations reduced the number of survived specimens.
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The antioxidant capacity of sea buckthorn (Hippophae rhamnoides L.) berries depends on the genotype and harvest time
27-29.Views:246Berries of sea buckthorn (Hippophae rhamnoides L.) are characterized by increasing popularity due to their presumable healtheffects. The aim of this study was to compare the antioxidant capacity and total polyphenolic content in the berries of six Hungarian grown sea buckthorn genotypes and characterize the genetic variability in this trait. The harvest time of sea buckthorn berries affects the antioxidant capacity and total phenolic contents in berries of three popular cultivars of German origin. Berries harvested in October had higher antioxidant capacity compared with those harvested one month later. The extent of the difference was genotype-specific. Our analysis revealed a nearly 3-fold difference between the lowest and highest antioxidant capacities of the 6 tested genotypes with ‘Leikora’ showing the highest ferric reducing antioxidant power and total phenolic content. The TEAC values ranged between 1.76 and 3.13 mmol Trolox/100g fresh weight with Pető 1 and ‘Frugana’ having the highest values. The results presented in this study demonstrated that Hippophae rhamnoides berries possess in vitro antioxidant activity strongly determined by genotype but also influenced by harvest time.
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Analyses of Hungarian sour cherry germplasm with simple sequence repeat markers
27-31.Views:198Twenty-four sour cherry cultivars (genotypes), belonging to four cultivar groups were fingerprinted using microsatellite markers. All genotypes have been arisen from the Carpathian basin, which could be secondary gene centre of sour cherry, since its progenitor species, ground cherry and sweet cherry overlap here. Five SSR primer pairs, earlier used for fingerprinting Turkish sour cherry germplasm were tested. None of the five primer pairs showed any polymorphism within the cultivar groups. The primer pairs were able to distinguish between the cultivar groups. The Oblacsinszka and the Cigánymeggy cultivar groups were the most difficult to separate, while the Pándy cultivar group was the most distinguishable.
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Growth and productivity of plum cultivars on various rootstocks in intensive orchard
77-81.Views:315Trees of three plum cultivars (Stanley, Cacanska Lepotica and Althann's Gage) were planted at Szigetcsép experimental station in Spring 1994 and trained to slender spindle with the aim to test their growth, effect of productivity under not irrigated conditions and to evaluate the adaptability of rootstock/scion combinations to intensive orchards. As control, trees on Myrobalan C 162/A (P. cerasifera) seedling are planted. In the trial two rootstocks are from Slovakia: Myrobalan MY-KL-A (red leaf) and Myrobalan MY-BO-1, vegetatively propageted. Further on two French rootstocks, the Marianna GF 8-1: Marianna plum (P. cerasifera x P munsoniana) and the Sainte Julien GF 655/2 (P. insititia) were involved. The Hungarian bred plum Fehér besztercei (P. domestica), which is recommended as apricot rootstock is also tested. Rootstocks MY-BO-1 and Fehér besztercei were planted with cultivar Stanley only. Trees were planted to a spacing of 5x3 m trained to slender spindle with 3-4 permanent basal branches. After yield start (1997) trees have been pruned only in summer, after harvest. In the alleyway the natural plant vegetation is mown, the orchard is not irrigated.
Based on tree size, vigorous rootstocks are Marianna GF 8-1 and Myrobalan C 162/A seedling, medium vigorous are MY-BO-I and MYKL-A; vegetative propageted myrobalan plums from Slovakia, while St. Julien GF 655/2 and Feller Besztercei proved to be growth reducing rootstocks. No significant difference between the rootstocks was found in turning to bearing. Under non-irrigated condition at Szigetcsép, cultivar Stanley produced the highest yield per area unit on vigorous rootstock (GF 8-1). The cultivar Althann's Gage produced the highest yield efficiency on Marianna GF 8-1 and they were healthy in the last 10 years. The symptoms of Althann's Gage trees on MY-KL-A rootstock indicate a possible incompatibility. The average fruit weight was significantly influenced by crop load on cultivar Cacanska lepotica, while no statistically proved differences were found on Stanley and Althann's Gage. The Cacanska lepotica trees produced significantly lower yield and larger fruit weight on St. Julien GF 655/2 rootstock. Adaptability to spindle training system depends on vigour of scion/rootstock combination: low or medium vigour cultivars (C. lepotica, Stanley) are good choice for spindle training systems even on vigorous rootstock; while the St. Julien GF 655/2 can be recommended only for vigorous Althann's Gage under our soil and climate conditions.
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Evaluation of lifespan values of six tree taxa in city streets
25-27.Views:290The aim of this study was to evaluate lifespan values of trees in tree-row system in the streets of Debrecen in two years (2009 and 2017). Six selected taxa (Pyrus calleryana, Acer tataricum, Sorbus intermedia, Magnolia kobus, Acer platanoides and Crataegus x lavalleei) were estimated for the following lifespan parameters: i) trunk diameter (cm), ii) tree crown size (m), iii) trunk status (in 0-5 grades), iv) tree crown status (in 0-5 grades), and v) estimated tree viability (in 0-5 grades). Our results showed that the largest were achieved for Pyrus calleryana and the lowest for Acer tataricum. The largest tree crown diameters were achieved for Acer platanoides and the lowest Magnolia kobus. The best trunk statuses by 2017 were achieved for Pyrus calleryana and the worst Crataegus x lavalleei. The best tree crown statuses by 2017 were achieved for Pyrus calleryana and the worst for Acer tataricum. The best estimated tree viability status was achieved for two taxa (Pyrus calleryana and Acer platanoides). Overall tree values were 2.73 times higher in 2017 compared to 2009. In summary, this study demonstrated the role of appropriate choice of tree taxa for a tree-row system under city street conditions.
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Concentration of ‘Oblachinska’ sour cherry juice using osmotic distillation
31-34.Views:230Sour cherry is one of the most famous and characteristic Hungarian fruit what is also commonly used in food production as a colour additive. The sour cherry cultivar, ‘Oblachinska’ was shown to be a rich source of antioxidant compounds, including mainly anthocyanins. Valuable compounds in fruit juice – vitamins, polyphyenols etc. –are heat-sensitive molecules, which should be taken into account during the process of concentration to prevent degradation. Osmotic distillation seems a suitable option to product high quality sour cherry juice because this process does not require high temperature or pressure. Raw juice with approximately 15°Brix was used for the experiment and tried to concentrate up to 60° Brix where the water activity low enough to inhibit the microbiological deterioration. Before and after the process, TPC (total phenolic compounds) and FRAP (ferric reducing antioxidant power) was measured using spectrophotometric methods to determine the effect of the osmotic distillation. Our results point out that osmotic distillation is a promising method to concentrate sour cherry juice and prevent the loss of valuable compounds.
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Promising white poplar (Populus alba L.) clones in sandy ridges between the rivers Danube and Tisza in Hungary
113-116.Views:174White poplar is a native stand-forming tree species in Hungary, covering 3.1 per cent of the forested area. More than 70 per cent of the white poplar stands can be found on calcareous sandy sites in the Danube—Tisza region, so they play a significant role in the poplar management of this part of the country. The most important task ahead of Hungarian poplar growers is to improve the quality of poplar stands and plantations based on selecting new clones and cultivars. The growth and yield of four promising white poplar clones was evaluated on a marginal site in central Hungary. The clones `1-1 425-4' (Populus alba x Populus alba), and 11 758' (Populus alba Mosonmagyaróvár 124) seem to be suitable for wood production, while the 427-3' (Populus alba x Populus alba cv. Bolleana) and 422-9' (Populus alba x Populus grandidentata) clones (with decorative stem form) could be better used for tree lines and ornamental plantations.
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Microsatellite based identification of grapevine cultivars traditional in Hungary and in the Carpathian Basin
71-73.Views:137Grapevine cultivars and clones traditional in Hungary and in the Carpathian Basin are maintained in the genetic collection of the Corvinus University of Budapest. Mostly ancient varieties and clones were genotyped using microsatellite loci. The investigated 6 loci were sufficient to distinguish all cultivars. Some clones could also be separated based mostly on the variable VVS2 loci. For 17 out of the 31 investigated cultivars this is the first report on characterization of the polymorphism of the allele lengths by microsatellite markers on loci: VVS2, VVMD7, VVMD27, vrZAG62.
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Determination of biogenic amine - content of natural wines
91-95.Views:161Nowadays the consumption of natural wines has been increased. In parallel with the higher demand, the study of the parameters of natural wines has also developed. The amines being produced during the decarboxylization procedure of amino acids could be one of the most important features of natural wines. The objective of this study was to measure the biogenic amine content of natural wines from 2000 and 2001. We have determined that the biogenic amine content of natural wines is not different from the biogenic amine composition of normal wines. There was not any considerable difference between the years. In complience with the average tendency, red wines contain higher histamine-, tiramine- and serotonin values than white wines.
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Tending operation models for black locust (Robinia pseudoacacia L.) stands growing on sandy soils in Hungary
55-57.Views:204A more intensive integrated research and development approach to the work carried out on the growth on sandy soils of stands of black locust (Robinia pseudoacacia L.) has been adopted in recent years, revealing several factors influencing stand growth. The fact that certain ecological factors influencing fundamentally the growth of trees have become unfavourable in Hungary in recent years has led to the more extensive use of black locust in the course of afforestation and forest regeneration schemes. The study presents a new, simplified tending operation model for black locust stands and age, growing space and target diameter models suitable for qualitaty log production and for mass assortments. The simplicity of these practice-oriented
models may foster the qualitative development of black locust management in Hungary and in some other countries where this tree species may gain greater acceptance by landowners and the forest industry. -
Comparative study of sour cherry samples for their anthocyanin content measured by tools of the laboratory and by portable equipments
29-31.Views:247The inner quality of fruits depends on many components. The analysis of pharmacologically important fruit species as the sour cherry consumed as a common food is an expensive task, mostly requiring an equipped laboratory. In sour cherry, one of its valuable components, the anthocyanin content deserves special attention. A portable analytical implement, which has been developed by our team measures the anthocyanin content on a scale of 1024 PharMgrades. The system is a member of the UVEX family of implements working with a microprocessor and performs the measurement quickly. The portable variant (UVEX-ML-1) does not require much training, the laboratory variant (UVEX UL-1) performs a large quantity of tests under modest laboratory conditions. Preparation of the samples is easily done, the necessary elements are available in the trade. The reagent is easily stored and dosed. The system was tested and proved to be of sufficient precision and the result showed acceptable variance according to the checks performed by a spectrophotometer in the laboratory.
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Influence of abiotic factors on the green mass and seed productivity of the raw material of Levisticum оfficinalе c. Koch in the conditions of Transcarpathia
46-51.Views:244For the wide implementation of Levisticum officinalе C. Koch in Transcarpathia into production it is necessary to widen its sort composition, as it is rather poor in Ukraine. Solving this problem is possible by enriching the source material with the samples of different ecological and/or geographical origin, by studying the influence of abiotic factors on the formation of green mass and seed productivity, separating the specimens best adapted to the conditions of growing, involving them into the selection process and creating new, highly productive sorts on this basis. The scientists of the Transcarpathian State Agricultural Experimental Station (TSAES) conducted researches (during 2011-2016) on the adaptive potential of the source material of the type of Levisticum officinalе C. Koch, which counts seven samples (Ukraine – 5 samples, Poland – 1, and Romania – 1), the selection work on creating new varieties for the given region is being done. The investigations were made on soddy podzolic gleyed low-humus soils with low content of nutrients. The results were obtained on the basis of generally accepted methods of investigation, i.e.: laboratory, field, visual, measuring-weighing, biochemical, mathematically statistic, and comparative-calculating. In the conditions of Transcarpathia the spicy greens of the high quality L. officinalе C. Koch are being gathered on days 25-30, the output of the essential oil makes up from 0.72 tо 1.89% on c.d.s., depending on the climate conditions of growing. The plants pass through all the stages of development, the bush is 55.4 to 92.7 сm in height, the plants form a big amount of rosette-shaped stems (11-34 pieces), the productivity of spicy greens is between 9.9 and 42.1 t hа1. The scientists of TSAES created the following sorts – Mriya (А. с. 06131 Ukraine, first registered in the State register of plant varieties of Ukraine) and Coral (Pat. on the variety № 140830). Economic effect from growing of the Coral variety in the conditions of Transcarpathia is 10230 UAH/ha and the level of profitability is 28.4%. The geographic position and agroclimatic conditions of Transcarpathia, as well as the unpretentiousness of L. officinalе C. Koch. tо the conditions of growing contribute to the enrichment of the local flora with useful plants; and the rich chemical composition contributes to the spread of this culture in the given region.
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Effects of different rootstocks on the morphologic parameters of watermelon grafts during transplant production
69-73.Views:165Before Hungary joined the EU watermelon production was a key sector of the Hungarian vegetable industry. Its production area shrank by nearly 60 % since 2004, it is only 5000 ha today. Stable and reliable market failed to evolve in the last years and extreme weather events occurred more frequently. An unfavourable growing season can notably affect the production volume of the next season. Information gathered in the last years support the assumption, that suitable stocks can help eliminate the issues caused by extreme weather or short-term crop rotation. Choosing the right grafted or non-grafted transplant is not enough any more, growers also have to select stock. In fruit and vine production choosing the stock, which is the most suitable for the technology is evident and this approach should be widely adapted by watermelon growers as well. In this research we investigated the growth parameters of different watermelon stocks and their effect on the scion during the transplant production. Measurements were carried out on seven different stock-scion combinations of the scion ’Topgun F1’ hybrid. Two of the stocks were interspecific (Cucurbita maxima × Cucurbita moschata) and five stocks were Lagenaria-type (Lagenaria siceraria) stocks. We made and recorded observations of the transplants’ length (cm), diameter of the stock and scion (mm), number of leaves (piece), root length (cm), root mass (g) and shoot mass (g). We concluded that early pricking out of transplants with interspecific stocks is successful due to their hardiness and vigorous growth. The effect of the ’Shintosa camelforce F1’ interspecific stock was outstanding. Furthermore, the results confirmed the practical observation that the root system of interspecific stocks grow vertically more allowing them to take up water and nutrients from lower soil layers in poor sandy soils. From the Lagenaria group the root system of the ’DG-01 F1’ was similar to the interspecific stocks’. It can be grown even in sandy soil, however other Lagenaria-type stocks are more suitable for production in hard soils with nutrient supply because of their compact root system. This study can serve as a basis for further research in the topic that can conduce to site- and technology-specific stock selection.
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International competitive analysis of wine producer small and midsize companies on enterprise resource management
23-25.Views:154In our research using primary data sources we are searching for satisfactory evidences, that the impressive economical growth of China has a strong impact on Chinese grape and wine production, wine market. However grape cultivation has long tradition in China, modem grape wine and wine production has only started 20 years ago. During my research I have collected the very rare and unreliable information on this theme to make a picture of Chinese grape and wine production. Using primary sources describe the present situation of Chinese grape industry, verify the supposition that the rise of Chinese grape crop land includes the growth of the total size of wine grape plantations. After this verification I make a comparative analysis of Chinese and Hungarian grape cultivation costs, to confirm, that grape and wine producing is a more requital activity in China than in Hungary.
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The effects of growth regulators in proliferation of Sorbus redliana 'Burokvölgy'
77-83.Views:191The Hungarian cultivar Sorbus redliana 'Burokvölgy' was proliferated on Murashige and Skoog (MS, 1962) medium with half-strength macroelements and 100 mg/1 meso-inositol, 20 g/1 sucrose, 11 g/1 agar-agar. Different combinations of kinetin (KIN), metatopolin (mT), benzyladenine (BA), benzyladenine-ribosid (BAR) and indolebutiric acid (IBA) were tested, and pH was adjusted to 5.6 every case using KOH. The cultures were incubated at 20-24 °C in 8/16 hours dark/light photoperiod for 50-52 days. The main aim of our research was to find the optimal growth regulator and its optimum concentration. Purthermore, to determine the chlorophyll contents of the in vitro propagated plants' leaves. During the proliferation, the highest number of shoots were observed in the case of using BA + IBA, and on the medium containing 0.75 mg/I BA + 0.05 mg/1 IBA 8.93 shoots were found. The addition of KIN + IBA decreased the number of shoots and increased the sizes of leaves — the widest (11.2 mm) and longest (17.8 mm) leaves were obtained on the medium containing 1.00 mg/I KIN + 0.05 mg/1 IBA. The longest shoots (36.46 mm) were found in the case of applying 0.75 mg/1 BAR + 0.05 mg/I IBA. The BA + KIN + IBA combination resulted the shortest shoots. Sometimes not only shoot regeneration but spontaneous rooting was observed during the multiplication. The highest chlorophyll content (1.569 mg/g total chlorophyll, 1.132 mg/g chlorophyll-a, 0.437 mg/g chlorophyll-b) was obtained in the presence of 1.0 mg/I KIN + 0.05 mg/1 IBA.
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Prediction infection risk on the basis of weather-related factors and Erwinia amylovora colonization in apple and pear flowers
39-54.Views:156Current infection risk prediction models utilize environmental parameters and field records, but do not take into account the estimated inoculum potential within the orchard. The object of this study was to survey the accuracy of three simple prediction methods under Hungarian climatic conditions, which could easily be used by the farmers. We also tested whether the accuracy of infection risk predictions can be improved by taking into consideration the incidence and/or rate of flower colonization by Erwinia amylovora.
After preliminary investigations in 1999-2001, data concerning the weather-related infection risk were recorded in 5 apple and 1 pear orchards in 2002, and in 12 apple and I pear orchards in 2003. The weather data were processed by the easy-to-use risk assessment models of the mean temperature prediction line (MTL), Smith's Cougarblight 98C and Billing's integrated system (BIS), and by the MaryblytTM 4.3 computer-assisted model for reference. The population size of E. amylovora in the flower samples was estimated within an order of magnitude by PCR.
For all years and orchards tested, Maryblyt indicated 35 days on which there was an acute infection risk. The same days were indicated by all 3 methods in 23 cases (66%), 8 days were indicated by 2 methods (23%) and 4 days were indicated by 1 method only. A similarly good correlation was found for prediction of the date of the first massive infection risk: in 2003, for instance, there was a perfectly consistent prediction by all 4 models in 9 of the 13 participating orchards. A coincidental forecast was provided by 3 of the 4 models in the other 4 orchards.
The results indicate that any of the risk assessment models could provide an increased accuracy of the actual infection risk prediction if combined with an estimation of the incidence of Erwinia amylovora colonization in the open flowers. We found no convincing differences in the size of the epiphytic population in flowers of cultivars possessing high or low susceptibility to Erwinia amylovora.
We conclude that the easy-to-use methods tested could be used by the fanners to recognize weather-related risks, especially when coupled with an estimation of the proportion of the pathogen-infested flowers. This local prediction would provide rapid information (faster than the regional forecast systems) specifically for a given orchard.
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Above-ground dendromass of sprouted black locust energy plantations: a case study
19-21.Views:239Black locust (Robinia pseudoacacia L.) can be considered as one of the most suitable tree species for establishing energy plantations due to its favourable growing characteristics such as vigorous growing potential in the juvenile phase, excellent coppicing ability, a relatively high resistance to pests. Based on national and international test results the mean annual increment of oven-dry weight of energy plantations regenerated by coppicing generally exceeds the first cycle plantations established by seedlings.
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New sweet cherry cultivars in intensive plantings
13-16.Views:314The study took place in the largest sweet cherry plantation in West Hungary. The purpose has been the identification of those varieties, which will be suitable for intense cultivation, early fruiting and excellent fruit quality, moreover, the selection of the optimal phytotechnical procedures. At the same time, scion-rootstock combinations have been tested also from the point of view of growing intensity and fruiting in high-density plantation. The dense planting is induced to start fruiting early and yield regularly by special methods.Yielding was stimulated by maintaining the balance of vegetative-generative growth by binding the shoots, by summer pruning, by cuts on the trunk and root pruning. Best experiences have been found in yield and quality in the following varieties: Canada Giant, Carmen, Firm Red, Giant Red, Katalin, Kordia, Regina. Dense planting has been feasible also on vigorous rootstock, like P. mahaleb. Dwarfing rootstocks like P-HL-A, Gisela 6, accelerate the formation of flower buds and yielding earlier with fruits of adequate size. ‘Firm Red’ and ‘Giant Red’ excelled with their large fruit (>27 mm diameter) in all combinations, thus being promising under Hungarian conditions.