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Identification of cow’s and buffalo’s milk and dairy product using a DNA-based method
279-282Views:176Aim of our study was the optimization of a DNA method, that is appropriate for reliable, low cost identification of animal species in milk and dairy product (cheese) and to determine the ratio of species. Mitochondrial DNA was used in our work to analyse buffalo/cow milk mixtures contained different ratio of bovine milk such as 0.1%, 0.5%, 1%, 1.5%, 2%, 5%, 10%, 15% (v/v%). Buffalo cheese were produced using buffalo and cows milk (0%, 2%, 5%, 10%, 15% – v/v% cows milk in buffalo milk). In case of milk mixtures, using species specific primers, the PCR assay showed a 0.5 v/v% detection limit. Cattle, in the buffalo/cows milk 99.9/0.1 v/v% mixture, was not detectable. The identification of buffalo and cows DNA in cheese was successful. The intensity of eletroforetic PCR fragment indicated the increase of cow milk ratio in milk and cheese samples as well.
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Modern Food Safety and Product Identification
339-345Views:83In the beginning of the 21st century the matter of food safety plays an accentuated role in the food industry. Important issues in this topic are the risk of bioterrorism, impurities in the food chain and the ascendancy of customer needs. The solutions of these problems are the introduction of modern quality assurance systems, traceability and identification of products. I review in this paper the possibilities of these systems, the potential advantages and incidental costs. In the tight frame of this paper – omitting the descriptions of technologies – I discuss the most important criterions of systems, which may be able to solve today’s quality food industry problems.
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Identification of environmental costs for agricultural companies – methodological questions and problems
177-183Views:81It is the consensus in the economic literature that corporate leaders do not know accurately the magnitude of environmental costs in their firms, and this lack of information is often a barrier to making environmentally-friendly decisions. For this reason, an important task of environmental accounting is identification of these costs, and a more realistic distribution of them among the products.
In this paper, we carried out identification of costs and expenditures for Hungarian agricultural companies.
We have dealt with this theme previously, but without detailing the related methodological questions. Detailed discussion is necessary because of the novelty of the subject (environmental accounting for agricultural firms).
Therefore, we elaborate the following questions: scope of determination of environmental costs and expenditures; methods for quantifying specific items; technique for their accounting. We also attempt to confront different viewpoints in the literature. Differences in accounting found between companies investigated are also presented.
The importance of our investigation is related to the significant increase in the number of environmental statutes that concern agricultural companies (e.g. rules for waste treatment, environmental taxation, introduction of charges and fees, regulation on liquid manure). Following these regulations puts an increasingly significant burden on the companies; therefore, the quantifications of environmental cost items has a growing
importance. -
Identification of the Slovak traditional cheese “Parenica” microflora
227-239Views:245Numerous studies have demonstrated the higher accuracy, faster time-to-results and lower costs provided by MALDI Biotyper systems compared to classical methods. In this study, the culturable population of total count of bacteria, enterococci, coliforms bacteria, lactic acid bacteria (LAB) and microscopic fungi and yeasts from cow’s dairy products was identified using the MALDI-TOF MS Biotyper. Altogether, 50 samples of the Slovak cheese “Parenica” were examined. Total numbers of bacteria were cultured on Plate count agar at 37 °C for 24–48 h, aerobically; enterococci were cultured on Enterococcus selective agar at 37 °C for 24–48 h, aerobically; coliforms bacteria were cultured on Violet Red Bile lactose agar at 37 °C for 24–48 h, aerobically. The LAB were cultured on MRS (Main Rogosa agar), MSE and APT agar at 30 °C in microaerophilic conditions. The microscopic fungi and yeasts were cultured on Malt extract agar at 25 °C for 5 days, aerobically. Isolated strains (total 669) were subjected to identification by the MALDI-TOF MS. Among total count the identified bacteria mostly were Acinetobacter baumannii, Bacillus cereus, Micrococcus luteus and Staphylococcus warneri. Escherichia coli and Enterobacter cloacae were the most abundant coliform bacteria representatives identified. Coliform bacteria included Citrobacter, Hafnia and Klebsiella. Altogether three genera belonged to the LAB – Lactobacillus, Lactococcus and Leuconostoc were identified with Lactococcus lactis, Lactobacillus plantarum, Lactobacillus coryniformis, L. fructivorans and Leuconostoc mesenteroides were considered as the dominated LAB species in dairy products. Among yeasts, Kluyveromyces lactis, Candida zeylanoides and Yarrowia lipolytica were among the most isolated.
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Identification of Hucul mare families by mtDNA markers
75-79Views:177Hundred animal species have disappeared during the last century. By this time, approximately one-third of domestic animals have been in the endangered category. Hucul horses are also in this category; furthermore saving the genetic diversity beside the race preservation is an important challenge as well. The number of mares and stallions is only one of the expressive elements of genetic diversity; together with their quality determine the genetic variability of this breed. Beyond that, if an exact breed can originates from more founders, it can be more renewed genetically. Stud book documents these data by registering the mare families and stallions’ genealogical lineage. Molecular genetics, especially mitochondrial DNA analysis can make the precise identification of mare families possible. As a result of these molecular based methods, protection of genetic diversity, as well as breed preservation became more reliable. After the primer designing, the optimal primer pair was chosen which targets a 1092 bp length DNA sequence in the cytochrome b region. After the successful PCR optimalisation, we determined 170 Hucul mares’ sequences. According to our results, the samples compose ten haplotypes, which are much less, than the registered number of mare families in the stud book. Further investigations are needed to reach more representative results, and drawn the further consequences.
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Accuracy of Location Identification by GPS and Possibilities for its Application in Agriculture
157-160Views:86A description of the design and operating principle of the GPS system and the explanation of key terms of telemetric and chronometric measurements is followed by several examples characteristic of the results of factors which may influence accuracy of the identification of geographic location by satellites. In addition, results of accuracy testing of absolute GPS measurements and improvements of accuracy experienced and attributable to the alteration of the periods used for establishing average values are highlighted. Furthermore, we examined certain practical possibilities for the use of GPS systems in agriculture, emphasizing the advantages hidden in the use of GIS-GPS integration, crop mapping, soil maps and gross produce maps, in the application of the VRT (Variable Rate Technology) and in combinations of the same.
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Isolation and identification of endophytic fungi connected to Grapevine Diseases, from the Tokaj wine region, Hungary
61-66Views:175Grapevine Trunk Diseases (GTD) is one of the most important diseases in vineyards worldwide, which can be found in Hungarian vineyards as well. In Hungarian wine regions there is very little information about the occurrence of pathogens which cause GTD, in case of Tokaj wine region there is no knowledge about that, what kind of pathogens can be found in the vineyards.
The objective of our research is to assess the situation and occurrence of GTD in Tokaj wine region in cooperation with local specialists, as well as identification of pathogens which were isolated from the diseased trunks by morphological and genetic basis.
We were able to isolate endophytic fungi from all sampled grape trunk. The majority of them were determined as Diplodia seriata not only based on colony morphology, but also determined by rDNA sequences.
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The factors affecting the proliferation of mould fungi and mycotoxin production during the storage of wheat and the identification methods of the appearing branches
129-133Views:124Nowadays, it is often suggested that, we should eat products made with whole grain cereals, despite of the fact that it raises the risk of consuming wheat products infected by mold and their toxins originated from the plough-lands and the stocks.
Two third of the cultivated fields in Hungary are planted with cereals. The most alarming problem for food and feed security is caused by the Fusarium species. The greatest problem of all is caused by the mycotoxins. When they get into the food chain they can be a serious threat to public health. In addition, we have to face up to the problem of the effects of global warming that influence the growth of microbial infections in different ways.
In this article we tried to summarize the effect of climate change on molds, the factors which have effect on growing and mycotoxin producing of molds and the identification methods of molds. -
Chromatometric comparison of Eurasian Collared Dove (Streptopelia decaocto) and Feral Pigeon (Columba livia domestica) feathers
19-22Views:189Chromatometric examination of the plumage of birds is a poorly researched topic. We have approached this issue in primarily aspect of differences in plumage of species. Moulted feathers sample collection method has been increasingly used. Reliable identification of feathers becomes an increasingly important issue, hence need for an exact measurement-based methodology. Eurasian Collared Dove (Streptopelia decaocto) and Feral Pigeon (Columba livia domestica) primary, secondary and tail feathers were studied. Chromatometric parameters of feathers were measured in CIELAB color system and then statistical analysis (Independent samples t-test, Descriptive Statistics, Discriminant Analysis) was performed to compare the two species. Instrumental measurements has been confirmed the high similarity between colors of the two species, however species specific differences were also found. Lightless (L*) value were significantly characteristic of particular species, while the red/green (a*) and yellow/blue (b*) value had lower Predictive Power. We identified feathers and the variables which useable to separate the two species and determined the associated Confidence Intervals of these values. Our results may draw attention to a new potential direction for exact identification of the moulted feathers during sample collection.
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A simple but powerful tool for the assessment of financial progress in EU co-funded grant programmes – Simple Progress Overview Tool (SPOT)
67-72Views:126Mid-term evaluation of grant programmes always aim to assess the financial progress of the programme. Progress to date assessment is conducted via addressing three issues: by overviewing progress so far, by exploring the reasons behind the current level of progress, and by answering the specific evaluation questions formulated as a result of progress overview. The Simple Progress Overview Tool is a method and a tool facilitating the overview of financial progress, supporting the exploration of reasons behind the current level of progress and enabling the identification of targeted further evaluation questions. The methodology applies the Du Pont approach of division to factors, in which the variable describing a phenomenon is divided into the multiplication of factors (milestone stages of the process) influencing the variable. Also, this division to factors facilitates the identification of reasons behind progress. The factors carry individually meaningful information to the evaluation of progress. This method was used by many EU member states including the Czech Republic, Romania, and Malta for the evaluation of progress in the course of the mid-term evaluation of their National Strategic Reference Frameworks.
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Use of molecular marker methods in the classification of bamboo taxa: A review
51-59Views:188Bamboo plants are currently attractive to researchers because of their versatile uses. Understanding the bamboos’ genetic level is needed to develop new varieties. Taxonomic identification is the basis for plant development. Bamboos were identified as their taxonomical morphological characters which are dependent on environmental factors. Molecular Marker techniques can be used to perform accurate genotype identification, which can be used for genetic diversity analyses. The RFLP, RAPD, AFLP, SSR, ISSR, iPBS, SCARS, SCoT, SRAP marker systems have been shown to be able to efficiently determine the genetic diversity of bamboo species based on genotyping. This paper summarizes research that aims to analyze the genetic diversity of bamboo species on a molecular basis.
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Identification and biological examination of some inland Monilinia species
27-29Views:130The aim of this study was to identify and biologically analyse some Monilinia species from Hungary. 146 M. fructigena and 28 M. laxa species out 174 infectious fruit from all over the country were used for the study. For further study 10 isolates were used and apple fruit was inoculated with them according to Koch postulate. 1–2 mm ochre exogen stromas were observbed on infectious plant parts and growing signs on culture of all isolates were identical to M. fructigena. To affirm classical identification, isolates with molecular biological method were also prepared using PCR reaction. Control isolates of M. laxa, M. fructigena and M. fructicola were used. The size of PCR product showed that all isolates had a 415 bp band which was typical of M. fructigena. Results support the previous observation that M. fructigena and M. laxa species occure all over Hungary.
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Economic aspects of innovation in sheep breeding
33-36Views:80Innovation, as a factor influencing the success of farming, is of outstanding importance also in agriculture. Only those businesses (enterprises, companies) can be successful in the longrun which are able to adapt the new technological elements and to make their own developments occasionally and make them suitable for practical utilization.
The innovation activities performed by the enterprises, business organizations can be evaluated at firm (microeconomic) and national economy (macroeconomic) levels. In the case of sheep breeding also, a complex evaluation system should be applied, since this is a sector, which has significant rural development and social impacts. The innovation processes are analysed from the identification of the problem inducing research and development until the return of the invested resources. -
Indicators and main motivations of environmental awareness in the enterprises of the Hungarian processing industry
133-137Views:116This paper focuses on the corporate environmental performance and its motivating factors. The background of the study is a questionnaire survey covers 82 Hungarian manufacturing companies. The identification of the environmental performance based on the study of György Pataki and László Radácsi who create a group of environmental indicators. The motivating factors are several stakeholders of the companies who have affect on an organization's environmental actions. These motivations can be divided into internal and external factors. According to the questionnaire survey the combination of internal and external factors seems to the most important to the better environmental performance.
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A dual infection of two microscopic fungi on common milkweed (Asclepias syriaca) in Hajdúság region (East-Hungary)
189-195Views:174Common milkweed (Asclepias syriaca L.) is one of the most noxious and invasive weed species in Hungary. A. syriaca invades arable lands, horticultural and forestry plantations, natural and semi-natural habitats too. In cases of field crops it can cause considerable yield losses mostly in maize (2–10%), soybean (12–32%) and sorghum (4–29%), but only with high rate of coverage. It can also increase these problems that the common milkweed can be serve as reservoir and host for viruses, other pathogens and pests.
Because of the importance of common milkweed and in spite of demand to develop effective biological control, until now has not been developed a proper control program against A. syriaca. The aim of our research was to identify the necrotrophic fungal pathogens, which were involved in notable disease occurrence on this weed in different parts of Hajdúság region of Eastern-Hungary in 2016.
To the isolation of fungi from leaves and their identification were based on morphological colony characters on potato dextrose agar (PDA) and Sabouraud dextrose agar (SDA). To the description of conidia features were used PDA for Alternaria and synthetic low-nutrient agar (SNA) for Fusarium species, respectively. The examination of axenic cultures revealed that the fungi isolated from the leaves of common milkweed were Fusarium sporotrichioides and Alternaria alternata. -
The examination of roe deer feeding on agricultural and woody habitats in winter
23-26Views:107sorting because the micro organisms, which help the digestion of high fibre plants, are missing in his stomack, that is why they are mostly called „concentrate selectors” (Hoffmann, 1985, 1988, 1989).
These animals should mostly eat easily digestable plants with high nutrition level (pulses, buds, sprouts and flowers), and they are able to do this sorting because of their mouth size. In winter there is a lack of these plants, so the high selectivity occurs only when the feed is in abbundance.
Examining the amount and quality of vegetation available on the habitat of roe deer we can identify the species which can satisfy their feed demand. It is known, that roe deer as other large ruminants, from the plant abundance prefer certain plants and plant parts while there are others which are avoided. The identification of the eaten species and the rate of their occurrence in the feed is the first step to become acquainted with the interaction between animal and the surroundings.
On the examined territories there was significant difference between the disembowelled body masses. The does which come from the hunt Nagyszénás weighed 4-5 kilos more than the does from the Csongrád territory. The main feed components were present in different rate in the samples of the territories, in the Nagyszénás samples grain, dicotyledonous herbacous plants were dominant, and were eaten more. The high level of grain in the feed result in better condition and larger body mass. -
Evaluation of the correlations between the normalized difference vegetation index (NDVI) and yield in a seasoning paprika (Capsicum annuum L. var. longum) stand
45-49Views:280The aim of our examination was to evaluate the correlations between the normalized difference vegetation index (NDVI) and yield, as well as to examine the possibility of yield estimation basedon NDVI in a seasoning paprika population.
Significant correlations were observed during the examination of the correlation between NDVI and yield. Furthermore, it was concluded that yield can be estimated with a 6.6–8.3% mean error based on the regression equations. No significant difference was shown between the error of estimations performed with various regression types and that of the estimations performed at various dates. For this reason, the identification of the optimum estimation method and the determination of the optimum date for estimation call for further examinations.
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Mass occurrence of a Phoma-like fungus on common ragweed (Ambrosia artemisiifolia) in Hajdúság region, East Hungary
55-60Views:218Common ragweed (Ambrosia artemisiifolia L.) is one of the most important, allergenic weed species in Hungary. A. artemisiifolia invades both a broad range of often disturbed areas (brownfields) and either undisturbed ones like waste lands, roadsides, riverbanks and railway tracks. In field crops it can cause considerable yield losses mainly in sunflower, maize, cereals and soybean. In Hungary many inhabitants suffer from allergy caused by Ambrosia pollen which results a serious human-health risk. The aim of the control is to prevent flowering and seed propagation of A. artemisiifolia. Until now the occurrence of numerous pathogenic fungi which attack common ragweed plants have been identified in Hungary, however there is not yet available biological weed control program because of shortage in acceptable effectiveness, and endangering cultural plant species. During our weed surveys in the region of Hajdúság (East-Hungary) we found numerous common ragweed plants showing heavy necrotic lesions on leaves and stems. The objective of this study was to identify the fungus which was isolated from diseased tissues of common ragweed (A. artemisiifolia). The identification of fungus based on morphological characters of colonies and features of conidia and chlamydospores developed on malt extract agar (MEA) plates. After examination of axenic cultures we revealed that the fungus isolated from the leaves ands stems of common ragweed was a Phoma-like species.
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The potential of biological control on invasive weed species
73-75Views:75Sorghum halepense is one of the invasive species in Europe. This study was made to identify the morphology of fungi on invasive weed species samples on the roots of Sorghum halepense. The samples were collected in the region of Debrecen. The experiment was conducted under laboratory conditions to determine the microscopic form of fungi. The samples were put on PDA and for identification of fungi is based on the morphological characteristics of the features and colonies of conidia that were developed in Petri dishes.
The examination of the culture revealed that the fungus from the root of Sorghum halepense was Aspergillus niger. Pathogenicity and the relationship between the fungus and Sorghum halepense are still uncertain so in the future pathogenicity tests and re-isolations from plants are very important steps.
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Fusarium culmorum isolated from rhizosphere of wooly cupgrass (Eriochloa villosa) in Debrecen (East Hungary)
93-96Views:155Wooly cupgrass (Eriochloa villosa) is an East-Asian originated weed species and it has been spreaded worldwide by now. The first occurrence of this species in Hungary was observed and published in 2008 nearby Gesztely village (Borsod-Abaúj-Zemplén county, North-East Hungary) than in the summer of 2011 a significant population was discovered next to Debrecen city (Hajdú-Bihar county, East Hungary).
In 2013 this weed was also reported from Szentborbás village, Somogy county (South-West Hungary). These observations of spreading and its biological features (production of stolons and large number of seeds, moreover herbicide tolerance) indicate that wooly cupgrass (E. villosa) has a great potential of invasiveness, so it may become a hazardous weed not only in Hungary but in all over the world.
The objective of this study was to identify the fungus which was isolated from wooly cupgrass (E. villosa) root residue samples which were collected after maize harvesting on arable land in late autumn, near Debrecen. The identification of the fungus based on morphological characters of colonies and the features of conidia developed on potato dextrose agar (PDA) plates. After the examination of axenic culture we revealed that the fungus from rhizosphere of wooly cupgrass was Fusarium culmorum. Pathogenicity and/or endophytic relationship between the fungus and wooly cupgrass is still uncertain so pathogenicity tests and reisolations from plants are in progress.
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Investigation of the milk composition of three Tsigai sheep varieties, and the detection of cazein fractions of sheep milk by Reversed-Phase HPLC
13-22Views:90The measurements started in 2005, the author used the milk of 3 types of Tsigai Sheep that are suitable for milking, they examined the milk composition, the possible differences between the milk types, the coagulation characteristics, cheese processing, yielding characteristics and the transfer of solid content into cheese. From the large number of measurements, the results of milk composition examinations are described.
During the research the author examined the milk coagulation property of three types of Tsigai Sheep QTS 25 is a substance investigating instrument, and its primary mission is the analysis of the substance profile. The advantage of this instrument is the representation of the survey graphs from which the part of the substance characteristics could be directly read off. By the help of the applied mathematical and statistical operations we tried to establish connection between the congealment-hardness and the specialty of the different types of Tsigai Sheep.
In 2006 the author began the identification and qualification of the major sheep milk proteins by RP- HPLC method. She used a scientific article written by Bordin et al. The method is able to separate and quantify the seven major proteins. However Bordin’s procedure was developed using bovine milk, the author adapted it to sheep milk. -
Development directions of microregional spatial environments
61-64Views:136The question how economic, technological and infrastructural developments, as well as products and technologies contribute to the unity and constant strengthening of the society is still unanswered both in Europe and the whole world.
The current erosion of the society and the weakening social cohesion is a regrettable process. These phenomena are a barrier to real development processes and weaken the extension of the economic space; therefore, this negative process has to be stopped and turned into a positive direction.
For this reason, it is increasingly urgent to work out new, feasible solutions, since it has become obvious that solutions whose sole aim is to increase profit typically weaken the indispensable unity of the society, market positions, innovation processes, as well as natural and environmental harmony.
In this situation, we analysed and interpreted the disadvantaged and multiply disadvantaged microregional spatial environments, with special regard to the strategic developments that serve both the strengthening of the microsocieties of microregional spaces and the development of the economy and the extension of economic space.
The identification of disadvantaged microregional spaces is basically done by means of economic indexes, but it is also important to emphasise that these indexes can only be interpreted with social indexes, as they define the situation of the microregional space and the possible development directions together.
The constant strengthening of social unity (cohesion) and the related economic development is basically a new focal point and objective.
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Morphological Features of Two Poa Species on Different Soil Types in Seminatural Grasslands
35-39Views:70The Poa pratensis L. and the Poa angustifolia L. are close relative species whose morphological features vary greatly. Our goal was to examine how the inividuals from different soil types of these species differ statistically from one another, whether the morphological features of the variant species differ significantly, and whether they keep their specific features in dissimilar habitats.
The quantitative features of the populations developing on distinct soil types wich are statistically separate provide for the different phenotype forming effect of the soil types as variant habitats. There is connection between the soil types and the measurement of the ramets on every soil types. The morphometric values of P. pratensis – that are bigger in all habitats – show that this species genetically widely adapted. Significantly different morphological features were found, but because of the high environmental dependence of the morphological features and of the significantly different characteristics which reveal several overlaps between the two species, these characteristics cannot be considered as reliable identification keys. -
Evaluation of chickpea (Cicer arietinum L.) in response to salinity stress
105-110Views:247Soil salinity is a severe and expanding soil degradation problem that affects 80 million ha of arable lands globally. Chickpea (Cicer arietinum L.) is very sensitive to saline conditions; the most susceptible genotypes may die in just 25 mM NaCl in hydroponics. Approximately 8–10% yield loss in chickpea production is estimated due to salinity stress. However, it is still not established why chickpea is so susceptible to salt affection. Salinity (NaCl) impedes germination of seeds, though chickpea varieties considerably differ from one another in this respect. Some chickpea genotypes are more tolerant in the stage of germination, tolerating even 320 mM NaCl. The reasons of this variation are unrevealed; there is a shortage of knowledge about the germination abilities of chickpea genotypes in saline conditions. Nevertheless, the effect of salt stress on vegetative growth can be analysed in hydroponics, in pot or field conditions, regardless the experimental environment, the ranking of genotypes regarding salt resistance is coherent. Chickpea genotypes can be different in their ability to retain water, maybe under salt affection; the more salt tolerant lines can maintain higher water content in the shoots, while the more sensitive ones cannot. The identification of salt tolerant chickpea landraces based on developing genetic variability is a suitable strategy to combat against salinity problems arising in arid and semi-arid areas.