Articles
The identification of phylloquinone in cattle samples using T.L.C. method
Published
October 5, 2010
APA
Bara, L. (2010). The identification of phylloquinone in cattle samples using T.L.C. method. Acta Agraria Debreceniensis, (I), 75–78. https://doi.org/10.34101/ACTAAGRAR/I/8379
A case of hemorrhage of unknown origin was observed in cattle and liver samples were submitted for assay of Vitamin K or
possible cuomarol. After evaluating normal and reverse phase Thin Layer Chromatography (TLC) plates and different solvents,
reverse phase TLC plate with indicator and methylene chloride:methanol 70:30 were chosen for direct method of detection and
indication of Vitamin K.Gass Chromatography and Densitometry were used to quantitate the Vitamin K present in liver samples and
confirmation of Vitamin K was done by Mass Spectroscopy.
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