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The identification of phylloquinone in cattle samples using T.L.C. method
Published:
2010-10-05
https://doi.org/10.34101/ACTAAGRAR/I/8379
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Bara, L. (2010). The identification of phylloquinone in cattle samples using T.L.C. method. Acta Agraria Debreceniensis, I, 75-78. https://doi.org/10.34101/ACTAAGRAR/I/8379
Abstract
A case of hemorrhage of unknown origin was observed in cattle and liver samples were submitted for assay of Vitamin K or
possible cuomarol. After evaluating normal and reverse phase Thin Layer Chromatography (TLC) plates and different solvents,
reverse phase TLC plate with indicator and methylene chloride:methanol 70:30 were chosen for direct method of detection and
indication of Vitamin K.Gass Chromatography and Densitometry were used to quantitate the Vitamin K present in liver samples and
confirmation of Vitamin K was done by Mass Spectroscopy.