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• The identification of phylloquinone in cattle samples using T.L.C. method

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  Lucian Bara

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  A case of hemorrhage of unknown origin was observed in cattle and liver samples were submitted for assay of Vitamin K or
  possible cuomarol. After evaluating normal and reverse phase Thin Layer Chromatography (TLC) plates and different solvents,
  reverse phase TLC plate with indicator and methylene chloride:methanol 70:30 were chosen for direct method of detection and
  indication of Vitamin K.Gass Chromatography and Densitometry were used to quantitate the Vitamin K present in liver samples and
  confirmation of Vitamin K was done by Mass Spectroscopy.