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Comparison of the physiological responses of an oil-pumpkin cultivar and hybrid under different nitrogen supply conditions
65-69Views:190Cucurbita pepo var. styriaca is known as a medicinal crop among other cucurbits. The benefits of cucurbits fruits are very important in terms of human health, purification of blood, removal of constipation, digestion and supplying energy. The mutant styrian oil-pumpkin (Cucurbita pepo L. convar. Pepo var. styriaca Greb.) exposes a complete lack of lignification of the seed testa. For this reason, this kind of naked pumpkin seed is more consumable as snack than other seeds. The vegetative growth of plants is crucial for fruit production. Nitrogen is the most imperative element for the proper vegetative growth and development of plants which significantly increases and enhances yield and its quality by playing a vital role in the biochemical and physiological functions of plants.
Field studies were conducted to study the effect of nitrogen nutrition on certain physiological parameters and their differences between the styrian oil pumpkin hybrid and cultivar (Gleisdorfi Classic cultivar and GL Rustical hybrid). The small block experiment was carried out based on a factorial experiment with a completely randomised block design and four replications in the Research Institute of Nyíregyháza during the summer period of 2017 and 2018. Three different concentrations of nitrogen fertiliser was applied at sowing. Photosynthetic pigments (chlorophyll-a, -b and carotenoids) were analysed as one of the basic factor of efficient photosynthesis. The yields of two genotypes were also measured in all treatments as seed-weight. The contents of photosynthetic pigments were higher in the oil-pumpkin hybrid, mainly the carotenoids in 2018. The vegetation period of 2018 was drier than in 2017, the hybrid oil-pupmkin was more efficient in stress tolerance than the cultivar with higher carotenoids pool. The obtained yield was more pronounced in the oil-pumpkin hybrid than the cultivar in all examined years. The hybrid GL Rustical was more sensitive to nitrogen treatment than the cultivar Gleisdorfi Classic.
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Vegetative shoot growing and yield productivity of different plum cultivar and rootstocks combination
25-29Views:123We planted containers plum rootstocks and cultivar combinations for irrigation and rootstocks experiment. We planted Cacanska lepo tica, Katinka, Jojo, Topfive, Toptaste, Topper plum cultivar on Mirobalan, St Julien A, St Julien GF 655/2, Wavit, Wangenheim, and Fereley rootstocks. Before budding we measured the trunk diameter on trees, than I count the trunk cross area, we measured the high of trees, the high of crown, and the wide of crown, and counted the volume of crown from these data. We conclude the vigorous from the trunk cross area and the volume of crown. In the started growing less vigorous combinations look like Topfive/Wavit, Jojo/Mirobalan and Katinka/Mirobalan grafted on the basis trunk cross area and the volume of crown. In the vegetative period we measured the shoot growing on model branch every started of months. So we could determine the growing tendency. The smallest growing was Cacanska lepotika/Mirobalan.
In the flowering the grafted flowered in rich, excepted the Topfive cultivar on St Julien A, St Julien GF 655/2, and Fereley rootstocks, these didn’t flowered. The Topfive/Wavit combinations there were a richest flower.
In the harvest term we could pick up plum fruits from Topfive/Wavit combinations, and Cacanska lepotica, Jojo, Toptaste cultivar. And in addition the Topper cultivar was the highest yield on their all of rootstocks. -
Effect of different production types on the yield and ß-carotene content of sweet potato /cultivar Ásotthalmi- 12/
45-49Views:150Production of sweet potato is extending in Hungary, despite the fact that there is no field-specific production technology. Therefore, many growers cannot utilize potential yields and quality. The goal of this study was to determine the optimal production method of the Ásotthalmi 12, a Hungarian sweet potato cultivar which can adapt to the Hungarian climate. The effect of single and twin rows production on the yield of this cultivar was examined. The planting was carried out on June 24th, the harvesting on October 20th and the growing-season was 120 days long. Uniform nutrient supply was applied to the whole field experiment. During basic fertilization, 206 kg Knd 20 kg N, 36 kg P ha-1 were used on the soil. While forming the ridges, we used an additional 25 kg N, 45 kg P and 62.5 kg K ha-1. We adopted drip tape irrigation on the experimental field. After the planting, from the 8th week of the vegetation, another 21 kg ha-1 K, 10 kg ha-1 MgSO4, and 2 kg ha-1 Ca(NO3)2 was added in one dosage weekly, until the 13th week of vegetation. At the evaluation of the experiment, we examined the yield regarding the whole experimental plot. SPAD and fluorescence measurements were carried out on 08.16. 2019, 08.28.2019, and on 09.13.2019, during the watering break. To demonstrate the difference between the dates, we applied ANOVA and Tukey post-hoc tests. For the measurement of phytonutrients HPLC, a liquid chromatograph was used, where the carotenoid content of the Ásotthalmi 12 cultivar was determined in connection with the different production methods. Test results showed that twin rows production leads to a nearly 30% greater yield, than single row production. We determined, that the growth of yield correlates negatively to the ß-carotene content of the Ásotthalmi 12 cultivar sweet potato.
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Effect of Storage on Fruitquality of Apricot (Prunus Armeniaca)
164-169Views:102The quality of a product is determined by numerous characteristics. As quality characteristics are polygenic, they cannot be improved easily. Moreover, there often is a negative correlation between the different parameters in the case of apricot. The firmness of fruits decreases with increasing size. The year effect also has a great influence on the expression of the potential quality of the given cultivar.
Our examinations were carried out in 2004 at Boldogkőváralja, in the cold storage plant of the Northcot Ltd. We monitored the development of fruits from fructification until harvest, and also the quality changes during storage. 100 fruits were observed from each cultivar directly after harvest. We measured height, width, weight, firmness, dry matter content and seed weight. From each cultivar, 100 fruits were stored, of which 25 fruits were observed each week. The value and the dynamics of the changes in the parameters compared to the values at harvest varied greatly for the different cultivars. Strong correlations between the parameters have not been found within one cultivar either. The heavier fruits did not lose more weight, either in absolute value or relatively, than the lighter fruits. The increase in the firmness of fruits was not accompanied with a desirable decrease in the dry matter content. -
Contexts between apple orchards with various cultivar comparisons and the effect of ATS (ammoniumthiosulphate) on fruit thinning
35-38Views:143Our research focuses on a fruit thinning material that can also be used in apple production. This material reduces significantly the required manual labor of hand thinning by russeting the blossoms selectively. The ATS (ammonium-thiosulphate) acts as chemical desiccant contrary to the nowadays commonly used materials such as naphthalene acetic acid, naphthyl acetamide, benziladenin and ethylene, which affect the metabolic processes of the plant by regulating the hormone system. In our experimentals cultivar ’Pinova’ and ’Golden Reinders’ were treated with different concentrations of ATS. The effect of these doses on the fruit setting and the quality and quantity parameters of the fruits was studied. According to our results, in the case of cultivar ’Pinova’ the ATS did not have any detected effects at the concentration of 1.5%. Application of ATS at 3% decreased considerably the fruit setting and fruit yield, accordingly the mean fruit size improved. The response to treatment in the case cultivar ‘Golden Reinders’ does not have any similar consistent results.
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The impact of production methods and row orientation on carrot quality in the case of various cultivars
65-69Views:135Carrot is a wellknown and favoured, really important vegetable. Carrot’s cultivation is important, although its growing field has been reduced in last few years. The suitable cultivar and landstructure are essential to produce good quality carrot. The ridge cultivation is widely spread on plasted soils. At this type of cultivation relationship between line orientations and carrot quality is less studied. That is the reason we tried to examine in our experiment the relationship between ridge highness and line orientation (N-S and E-W) and carrot’s morphological features at different genotipes. The experimental was settled in the Experimental Garden of the University of Debrecen on limy chernozem soil by plain, raised bed and ridge cultivation in 2013. In the experiment we examined four longgrowing cultivars (Danvers 126, Fertődi vörös, Rekord, Chantenay). The sowing was at 24th April. The harvest was at 15th October, 2013. In the multi factorial (type of cultivation, line orientation, cultivar) experimental we explained the effect of treatments on carrot root shoulder diameter and root weight.
In our experiment we stated that line orientation had no significant effect on shoulder diameter at different cultivations. The only exception is Fertődi vörös which has reached the biggest shoulder diameter (5 cm <) at N-S direction on raised bed. By examining the carrot rootweight we stated that they were higher in raised bed and ridge cultivation than in plain cultivation with the exception of one cultivar (Chantenay). This carrot had found ideal environment for growing between each cultivation conditions. That is why we can state that if you grow carrot on plasted soil and there is no possibility to make a ridge, use short, tapered and rounded ending root type for successful growing.
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Correlation of pruning time and fruiting part differentiation of sweet cherry cultivars
131-134Views:79There are several extended studies in sweet cherry production in Hungary and all over the world i.e. for creation and maintainence of smaller tree crown and high density orchards. The use of suitable dwarf rootstocks for this fruit species are very limited. On one hand, most of the draf rootstocks do not cause enough growth reduction and on the other hand these rootstocks are get old very quikcly and their fruits become small, and therefore, they not serve the requirements for intensification. In summary, there is a need for those rootstock which are vital, regeneration enhanching and delay ageing. Due to ensuring above features, Prunus mahaleb is still an obvious solution for intesive production. Increasing intensification can be obtained by use of modernisation of technological elements and suitable cultivar choice.
According to this increasement of intensity through application of novel technological elements (timing, manner and severity of pruning) and selection of the proper cultivar is implement able. Important differences are experienced between sweet cherry cultivars in their growth attributes, light demand and dynamics of fall back in regenerative potencial of different aged wood parts. From this point knowledge of the abow detailed is very important in order to maintain rentability of already established plantations. Our work shows the formation of production part in
9-year old plantation with spike spindle and free spindle crown forms depending on pruning timing (winter, summer) and determining of their various effects. -
The role of cultivar susceptibility and vineyard age in GTD: examples from the Carpathian Basin
57-63Views:193Grapevine trunk diseases (GTDs) are among the most severe problems in viticulture worldwide. The exact etiology and the role of endophytic microorganisms is not known yet and there is no adequate protection or curative treatment against the disease. Hungarian wine regions are also affected by the disease, and there is restricted information about the rate of infection nation-wide and about the susceptibility of the Hungarian cultivars.
The main objectives of our research are to measure the symptom expression and the damage caused by GTDs, to understand the epidemiology and etiology of the disease to establish a foundation of a proper disease management.
Cultivar susceptibility groups were created with the aim to allocate some Hungarian cultivars and the role of vineyard age was also examined in symptom expression.
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Disease incidence of shot-hole disease of plum in two training systems
107-110Views:30Of the foliar diseases of European plum, Wilsonomyces carpopilus is the most commonly occurring fungal pathogen. The aim of this two-year study was to investigate the susceptibility of ‘Čačanska lepotica’ plum variety to shot-hole disease (Wilsonomyces carpophilus) in two different training system with 4 x 1.5 m and 6 x 3 m tree spacings. The obtained results showed that the cultivar is susceptible to this disease and by the end of the vegetational period disease incident was above 50% in both years in both tree spacings. In 2018 disease incidence was higher in both spacing than in 2019, reaching almost 90% at the 4 x 1.5 m tree spacing plot. There were few significant differences between high and low density tree spacings. The results highlighted the importance of inoculum accumulation late in the season.
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Effects of cultivar and fertilization on extensograph parameters of winter wheat flours
19-24Views:110In present paper we have examined the effect of mineral fertilization on the extensograph characteristics of wheat-flours. The baking quality of winter wheat is largely determined by cultivar, but it can be influenced by fertilization. Flours were from 4 cultivars grown at six nitrogen fertilizer. The average flour protein content of cultivars was very different (11.8, 13.0, 14.3, and 13.07%, respectively). We have found that the extensograph
properties of dough are affected by mineral fertilization, nevertheless, different cultivars distinctly react to the increase of fertilizer doses. We estimates the correlation between flour protein content and extensograph properties, based on our investigations, it became obvious that the correlation between flour protein content and extensograph parameters is really strong. -
Sequence stability at SSR, ISSR and mtDNA loci of common millet (Panicum miliaceum) from the middle ages
10-19Views:95Seed remains of medieval millet, recovered from a 15th century layer (King’s Palace, Budapest, Hungary), showed reddish yellow grain color after rehydrating on tissue culture medium that was close to grain color of modern cultivar Omszkoje. aDNA of medieval c. millet was extracted successfully, analyzed and compared to modern common millets by ISSR, SSR, CAPS and mtDNA. Analyses of fragments and sequences revealed
polymorphism at seven ISSR loci (22 alleles) and at the 5S-18S rDNA locus of mtDNA. CAPS analysis of the 5S-18S rDNA fragment revealed no SNPs in the restriction sites of six endonucleases TaqI, BsuRI, HinfI, MboI, AluI and RsaI. Sequence alignments of the restriction fragments RsaI also revealed
consensus sequence in the medieval sample compared to a modern variety. Morphological characterization of twenty common millet (Panicum miliaceum L., 2n=4×=36) cultivars and landraces revealed four distinct clusters which were apparently consistent with the grain colors of black, black and brown, red, yellow, and white. In the comparative AFLP, SSR and mtDNA analysis modern millet cv. ‘Topáz’ was used. AFLP analysis revealed that extensive DNA degradation had occurred in the 4th CENT. ancient millet resulting in only 2 (1.2%) AFLP fragments (98.8% degradation),
compared to the 15th CENT. medieval millet with 158 (40%) fragments (60% degradation) and modern millet cv. ‘Topáz’ with 264 fragments (100%). Eight AFLP fragments were sequenced after reamplification and cloning. Microsatellite (SSR) analysis at the nuclear gln4, sh1, rps28 and rps15 loci of the medieval DNA revealed one SNP (single nucleotide polymorphism) at the 29th position (A to G) of rps28 locus compared to modern millet.
Mitochondrial (mtDNA) fragment (MboI) amplified at the 5S-18S-rDNA locus in the medieval millet showed no molecular changes compared to modern millet. The results underline the significance of survived aDNA extraction and analysis of excavated seeds for comparative analysis and molecular reconstruction of ancient and extinct plant genotypes. An attempted phenotype reconstruction indicated that medieval common millet showed the closest morphological similarity to modern millet cultivar Omszkoje. -
Effect of M9, MM106 and Seedling Rootstocks on Sunburn-Sensitivity of 33 Apple Cultivars, and Sunburn Effects on Fruit Quality
236-247Views:87The purpose of this study was to investigate the sunburn-sensitivity of 33 apple cultivars grafted onto 3 rootstocks (M9, MM106 and seedling) in a commercial orchard at Nagykutas (in the western part of Hungary). The authors also searched for any relationship between fruit quality parameters and the frequency of sunburn on the fruit surface.
During the observations, the cultivars had rootstock-specific properties in respect to sunburn-susceptibility. Accordingly, the injury decreased in the order M9, MM106 and seedling rootstocks. The differences in the sensitivity were founded on the foliage-morphological characteristics of trees, caused by the growing vigour of the rootstocks. Accordingly, the highest value of sunburn injury was observed on M9 rootstock, because this rootstock has a dwarfing effect on the grafted main cultivars. Thus, the vegetative area of these trees grew very slowly and the foliage was not compact enough to protect the fruits from the strong rays. The largeness and density of the foliage increased in the order M9, MM106 and seedling rootstocks. Relationships were also demonstrated between the diameter of the upper part of crown, the size of leaves, the number of fruits per tree and the injury from sunburn.
The damage values showed, that the gravity of symptoms did not decrease below a well-defined level of dimension on fruit. Determined potential area of injury was necessary for the symptoms to become visible.
The authors categorized the cultivars in the respect of values of sunburn frequency: I. „Not sensitive”, II. „Moderately sensitive” and III. „Very sensitive” categories were constituted. Generally, the Gala cultivars showed low damage (or were free of symptoms), in contrast, Golden mutants suffered relative strongly. The most sensitive cultivar was Jonica on all three of rootstocks.
We searched for any relationship between the fruit quality parameters and the frequency of sunburn. A significant correlation was found in the cases of fruit weight and colour-coverage. The latter can be related to that fact that highly colour covered fruits are found on the peripherial part of crown, so these are exposed to stronger sun rays -
Examination of two artichoke cultivars under single year Hungarian climate conditions
112-115Views:75Based on the results of a previous experiment, we concluded that by choosing a suitable cultivar, it is possible to produce artichoke in a one-year cultivation system in Hungarian weather circumstances. The objective of the present experiment was to investigate the performance of two Californian artichoke cultivars (Green Globe, Imperial Star) under the local conditions. Experiments were conducted in 2002 and 2003, by using four
different sowing and two different planting dates (03.01. and 03.20. – planting 05.07.; 04.24. and 05.06. – planting 06.12.). For both cultivars, 100% of the plants initiated buds. In most of the cases, Imperial Star produced higher total and marketable yields than Green Globe did, while it formed bigger and more uniform buds. Our opinion is that the main reason for this result was that Imperial Star tolerated the dry and warm Hungarian summer much better. Irrespective of the variety by earlier sowing dates higher total yield was achieved. Based on our results, we found that under Hungarian circumstances, both cultivars could produce acceptable total yields. However, the weather circumstances of the given year influenced bud quality, and thus marketable yield, to a great extent. -
Evaluation of vegetative and generative characteristics of some ‘Gala’ cultivars
15-20Views:126The aim of this study was to evaluate the production characteristics of new ‘Gala’ apple cultivars (‘Gala Venus Fengal’, ‘Gala Decarli- Fendeca’, ‘Galaval’, ‘Jugala’, ‘Gala Schnitzer (S) Schniga’), which can be described with superior and earlier fruit surface coloration and with larger fruit size comparing with the origin cultivar, however they are less well known in our country. The place of our experimental is located in the Nyírség representing adequately the ecological conditions of the surrounding areas, so the gained results can be easily adapted in the biggest apple production site of Hungary. According to the results it can be stated that among the 3–4 years old ‘Gala’ trees, which will achieve the maximal yields just in the next years regarding yields the ‘Fengal’ and the ‘Fendeca’ cultivars are emerged (43–46 t ha-1).
Furthermore they reached also excellent fruit quality (70–75 mm diameter). The fruit quality of the ‘Galaval’ is also optimal, but the yields are notably smaller. The ‘Jugala’ produced lower yields and the coloration of the variety is also insufficient. In the case of the ‘Schniga’ the excessive increasing yields can cause quality loss easily.
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Nutrient Supply Effects on the Fruit Quality of Apple (Malus domestica Borkh.)
230-235Views:103The aim of our two year study is to research the effect of nutrient supply on apple fruit quality, and to explore the relationships between selected fruit quality parameters.
Observations were made in Kálmánháza (in the eastern part of Hungary), on a commercial apple orchard. In this experiment, we studied the nutrient supply reaction of four apple cultivars (Golden Delicious, Granny Smith, Idared and Jonathan Csány) under different N and NPK doses. The following fruit quality parameters were studied: fruit diameter, fruit height, fruit weight, flesh firmness, colour-coverage and we studied the density of foliage.
The research results showed that N fertilization has a great effect on fruit quality. This is shown in the cases of increase of fruit size (fruit diameter, fruit height, fruit weight). The increase is proportional with the N doses, accordingly the highest positive difference was observed by using 100 kg/ha N doses. It is important to note that moderate N doses (75 kg/ha) plus P and K additions also had positive effects. There approached the values of 100 kg/ha N, and even exceeded its values in the cultivar Golden Reinders. The increased N doses enlarged the standard deviation, on the other hand, this parameter was low in the cases of balanced NPK fertilization. The nutrient supply increased the vegetative area (density of foliage) in addition to the generative parts, in particular only N fertilizer. However the denser foliage hindered the growth of fruit weight and colour-coverage, and also decreased the fruit quality and the flesh firmness of cultivars, which have a negative effect on storageability.
A linear correlation was demonstrated between the fruit weight and colour-coverage, or between fruit weight and flesh firmness. The character of their relationship was similar, but the direction differed: high fruit weight was with high colour-coverage, but with low flesh firmness. -
Effect of ethephon levels and amino acids on the growth characteristics of oat crop
57-61Views:75A field experiment was conducted during the 2021-2022 season in an agricultural field in Basra Governorate. The aim was to study the effect of spraying ethephon and amino acids on the growth characteristics of oats (Avena sativa L.) Shifa cultivar. The experiment involved three levels of ethephon (0, 0.240, and 0.600 kg ha-1), represented by the symbols E0, E1, and E2 respectively, and two spray levels of amino acids (0 and 5 ml L-1), represented by the symbols A0 and A1. A factorial experiment was applied using the R.C.B.D (randomized complete block design) in a split plots arrangement with three replications. The results revealed significant differences in the levels of ethephon for most of the studied characteristics. The level of 0.240 kg ha-1 (E1) was superior in terms of chlorophyll content, crop growth rate, and the number of tillers, with increases of 39.07%, 39.26%, and 16.36%, respectively, compared to the control treatment (E0). Regarding the amino acids treatments, spraying at a concentration of 5 ml L-1 (A1) demonstrated significant superiority, resulting in the highest plant height, flag leaf area, chlorophyll content, and crop growth rate. This study concludes that amino acids play a crucial role in plant growth, and the combination of 5 ml L-1 amino acids with 0.240 kg ha-1 of ethephon significantly increased chlorophyll content and crop growth rate.
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The possibilities and limitations of organic fruit production
41-45Views:71In this review, direct and indirect technological elements of organic production are discussed. Today, there is a growing interest in production prepared without chemicals. We discuss the following issues: site selection, soil, rootstock and cultivar requirements, plant material, planting distances, crown formation, phytotechical operation, irrigation, soil tillage, soil covering and muchning, nutrition supply. Separate section deals with methods of plant protection.
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Gene resources for improvement of drought tolerance and yield quality in dry pea breeding
105-111Views:93The effectiveness of selection for improved drought tolerance and consumption quality in the progeny of crosses between pea cultivars with semi-leafless (afila) and normal leaves and different origins, respectively, were investigated. After single crosses, parent cultivars and F1, F2 and F3 generations were grown under non-irrigated conditions in the same trials. We created a colour scale from 1 to 9 to measure statistically the shade of seed colour. The tolerance of genotypes against high temperature was measured by the number of pods per plant. The 3:1 segregation
observed in the F2 generation of crosses between semi-leafless and conventional cultivars indicated that the semi-leafless character is determined by a recessive gene. In contrast, the ratios of conventional (Af) and semi-leafless (af) genotypes were 7:1 and 9:1 ratio in the progenies of crosses of Af × af. The genetic progress was effective for improving the seed quality in F3 generation from crosses Af x af where we found that multiple
dominant alleles controlled the orange colour of cotyledons and its high heritability (h2 A=0,63). Selection is more effective in producing the genotypes with high yield and normal leaves if the crosses were made between the western European cultivars such as semi-leafless Profi and Delta used as maternal cultivars and conventional Auralia cultivars. In this case, there were decreases in the consumption quality, such as seed size and shade of colour. The selection based on the seed weight of single plants for increasing drought tolerance seemed to be more effective in F4
strains with normal leaves originated from Czechoslovakian maternal cultivar Y228; however, the genetic progress in the improvement of seed size and colour quality was slow. -
Seedling morphology of different wheat genotypes at early stages under hydrocultural conditions
249-254Views:213Consuming “sprouted seeds” is one of the most important factors of a healthy diet. An experiment was conducted in the University of Debrecen, Research Centre of Nyíregyháza (Hungary) in 2014 to analyse some morphological traits of four winter wheat genotypes (Triticum aestivum L.) and one spelt (T. spelta) variety. Our results showed that the spelt wheat variety “Franckenkorn” could maintain higher root length throughout the experimental period. On average, both “Perbetei” and “Franckenkorn” varieties could maintain higher root number compared to the other varieties. The extensive breeding line “1401 HK” had the highest shoot length throughout the whole experiment, being significantly higher than the landrace variety “Perbetei” and both of the varieties “KG Bendegúz” and “KG Kunhalom”. It could be concluded that “KG Bendegúz” cultivar and “Perbetei” landrace seem to be the most suitable for aquaculture techniques. In addition, “1401 HK” breeding line can be the most suitable for the production of juice since the minimal required shoot length (12 cm) was achieved within the shortest period of time after sowing (9 days). This breeding line and “Franckenkorn” can also be suitable for production of “wheatgrass”, because it is consumed without roots. Further research is needed to evaluate nutritional values of these genotypes.
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Development of a new measurement method to determine plant antioxidant status
105-112Views:192The antioxidant capacity of 12 cultivar that were harvested in 2014, were determined by FRAP (Ferric Reducing Ability of Plasma), DPPH (1,1-diphenyl-2-picrylhydrazyl radical-scavenging activity) TEAC (Trolox Equivalent Antioxidant Capacity) and photochemiluminescence method. In sour cherry, the most antioxidant effects of natural bioactive compounds are anthocyanins. Our results show that the photochemiluminescence method is the most suitable to determine the antioxidant capacity of red soft fruits and tart cherries
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Does drought stress always negatively affect the yield and quality of soybean in Hungary?
37-40Views:208Globally-fluctuating climate imposed serious abiotic stresses on the agricultural sector, leading to noticeable, and sometimes disastrous, losses in yields and/or quality of crops; however, in certain cases, plants could survive stress with relatively low reductions, and sometimes even with some enhancements as a reaction to changed environment, especially in the case of mild stress. An experiment was conducted in 2017 and 2018 in Debrecen, Hungary to evaluate the mild drought stress influence on the yield and quality of three soybean cultivars. The results showed that both ES Pallador and Pedro cultivars could achieve more yield when subjected to mild drought conditions; however, protein concentration was enhanced in ES Pallador whereas slightly degraded in Pedro under drought. The cultivar Pannonia Kincse followed different trend; both yield and protein concentration were reduced under drought. Oil concentration of the three cultivars did not show significant changes; however, it always followed opposite trend to that of protein concentration. It could be concluded that both ES Pallador and Pedro are recommended to be grown under rain fed conditions in Debrecen, whereas Pannonia Kincse is recommended under irrigation conditions.
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Selection of powdery mildew resistant and susceptible grapevine genotypes with molecular markers
100-104Views:132Incorporation of competitive quality and resistance against the most important fungal diseases (powdery and downy mildew) in a cultivar is one of the most important aims of grapevine breeding. In the 20th century, the most advanced results in grapevine resistance breeding were achieved by French researchers. They used resistant cultivars in more than 30% of their growing areas. In these varieties, North American wild Vitis
species were the resistance gene sources. The discovery of immunity-like resistance of Muscadinia rotundifolia opened new perspectives in resistance breeding. M. rotundifolia harbours a dominant powdery mildew gene, providing resistance in highquality cultivars after back-crosses with V. vinifera varieties. M. rotundifolia has been involved in the Hungarian grape breeding programs since 1996, thanks to a French-Hungarian variety exchange. In addition to traditional selection methods, application of MAS (Marker Assisted Selection) based on various types of
molecular markers, can provide additional tools for these efforts. Run1 locus, responsible for powdery mildew resistance, was identified in Muscadinia rotundifolia. Molecular markers closely linked to this locus are very significant in screening progenies deriving from M. rotundifolia and V. vinifera crosses, making possible the discrimination between resistant and susceptible genotypes at DNA level. In our analyses BC5 progeny of {(M. rotundifola×V. vinifera) BC4}×Cardinal (V. vinifera) tested for powdery symptoms were analysed with PCR-RFLP (GLP1- 12P1P3) and microsatellite markers (VMC4f3.1, VMC8g9). Our results proved the applicability of the linked markers and reliability of marker assisted selection. -
Storage of wheat at high moisture
111-114Views:79Deterioration rates were determined for 15-19% moisture content wheat (Dropia cultivar) stored at constant temperatures.
Deterioration rates were determined by measuring germination capacity of the grain and respiration rates of grain. Safe storage
time was defined as the time for germination to decrease to 90%. Safe storage times of 19% m.c. wheat stored at constant
temperatures ranged from 2.5 d at 30 and 35°C to 37 d at 10°C. Deterioration rates of 19% m.c. wheat stored with a step decrease
in storage temperatures (35-25, 30-20, 25-20, and 20-15°C) were determined and safe storage times were satisfactorily predicted.
Safe storage times of 17% m.c. wheat were 5, 7, and 15 d at 35, 30, and 25°C, respectively. Respiration rates and germination
percentages of 15 and 16% m.c. wheat stored at 25°C remained constant for 70 d. The respiration rates of 17-19% m.c. wheat at
25°C increased while the germination percentages decreased with storage time. Germination dropped from 98 to 92-89% when the
dry matter losses were about 0.05% and visible mould occurred when the dry matter losses were about 0.1% in 17-19% m.c.
wheat. -
Examination of the usability of the MALDI-TOF method in sunflower genetic identity analyses
33-38Views:164Economically, one of our most important crop is the sunflower. Regarding its production it is essential to use top quality seeds. As the conditions of seed certification and the certification parameters are regulated by laws and ordinances, it is highly important to farmers and seed producers to detect seeds of low quality or dubious origins.
Nowadays, the examination of the cultivar homogenity of sunflower is based on a reference method of the International Seed Testing Association Rules International (ISTA), Chapter 8. This standard reference method uses ultrathin poliakrilamid isoelectric focusing gel electrophoresis (IEF-UTL, or simply IEF). With this work we have set out to compare the results of MALDI-TOF to the test results – used as reference results – achieved by this reference method. The aim is to develop a new, quick, cheap and reliable method. In this article I summarized the results of some of the experiments that will provide basis for my further work.
During our previous experiments we have concluded that out of four extracting agents we can get the most protein markers using NaCl acid buffer, 1 propanol buffer. -
Environmentally-benign plant protection possibilities against domestic Monilinia spp. in organic apple and stone fruit orchards
101-105Views:142In this study, possibilities of environmental-friendly plant protection against domestical brwon rot species were summarized for oecological pome and stone fruit orchards. Symtomps of the two most important brown rot species (Monilinia fructigena (Aderh. & Ruhl.) Honey and Monilinia laxa (Aderh. & Ruhl.) Honey) were described and then cultivar susceptibility to brown rot was discussed. Furthermore, mechanical, agrotecnical, biological, and other control possibilities (stone powders, plant extracts and restricted chemical materials) were shown.