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Comparative investigation on Hypericum perforatum L. populations of different origin
56-60.Views:223Widespread application for herbal medicines based on Hyperici herba has been experienced in the last few years, especially in the treatment of depression syndrome. As the wild origins could not satisfy the market demands neither in quantitative nor in qualitative respect, the necessity of the development of intensive growing methods has been raised. In the course of our investigations we intended to clear up the morphological and chemical variability among and within populations of different origin in order to start a new breeding program. According to our data, growth dynamics of populations could be characterised by a logistic curve. According to the time of flowering the populations formed early, middle and late groups. Morphological diversity among populations was measurable in differences of flower length, plant height, different leaf types and plant habit, according to which characteristics groups were distinguished. Generally, the accessions were the most homogenous in plant height (CV: 7-15%), followed by inflorescence-lengths (CV: 11-36%) and the least uniform characteristics proved to be the number of flowering shoots (CV: 14-59%). The greatest morphological heterogeneity was experienced in the accessions of wild origin as it has been expected. In the second vegetation period generally a much better homogeneity was obtained, than in the first year. The most outstanding accessions produced 1.2 t of dry flowers and 4 t of dry flowering shoots, calculating to one hectare area. The individual yields varied on a large scale in each population (CV: 18-70%).
The content of hypericin varied between 1,85 and 9,9 mg/g in 1996, and between 0,18 and 2,70 mg/g in 1997, showing high individual divergences. Flavonoid values -including first of all hyperosid, rutin and quercitrin - reached 17-39 mg/g in the first and 15-20 mg/g in the second year, respectively. Individual and seasonal variation was less than in hypericin. A joint high level of these two compound groups seems to have low frequencies.
The superior population were selected for further breeding.
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Evaluation of some Achillea L. accessions based on morphological, cytological and chemical characteristics
69-74.Views:168Taxonomical evaluation of six taxa of the Achillea aggregate was carried out to determine their potential for breeding and cultivation. We used complex morphological, cytological and chemical aspects of characterisation. Three taxa of tetraploid genom and high chamazulene contents (33-40% ess.oil) proved to be A. collina Becker. A strain of wild origin had also high -bisabolol content and high oil level, comparable with the selected cultivars. A population was identified as new chemotype of A. pannonica Scheele with typical morphological and cytological traits, a wide sesquiterpene spectrum but lacking of 1.8 cineole and -pinene. We found a mixed population which consisted of both A. collina and A. pannonica plants. A tetraploid, azuleneless taxon could be concluded as A. pratensis Saukel and Langer not described before in Hungary. The diverse performance of the populations calls the attention to the significance of controlled plant raw in phytopharmaceutical products.
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Effects of environmental factors on morphological and quality parameters of table beet root
139-146.Views:348In our trial morphological and quality parameters of 15 table root varieties were tested at 3 different sowing dates: 15 April, 9 July and 19 August 2010. In the trials the root shape of the varieties form the April sowing date approached most the regular spherical shape (diameter/length –1.0) which is favoured both by fresh market and the processing industry. In the July and August sowings the roots were elongated with reduced proportions. The highest red pigment content (betacyanin) was observed in the second sowing of July (>80 mg/100 g). In the late sowing (August, under plastic tent) a further 10–20 mg/100 g pigment increase was measured in relation to the earlier sowing dates of the same varieties. A similar trend could be observed in yellow pigments (vulgaxanthis) which proves a close correlation between the quantities of the 2 pigments (r=0.823). The highest vulgaxanthin content (103.3–124.18 mg/100 g) was obtained form roots of the late sowing harvested in December. Varieties reacted differently to temperature and so to sugar accumulation in the different sowing periods. In the July sowing higher water soluble solids content was measured on the mean of varieties (10.12 %) as compared to the April sowing (7.76%). Sensory evaluations included inner colour intensity (1–5), with ring (1–3) and taste (1–5) of the raw material evaluated by scoring. According to laboratory measurements better inner colour intensity was observed in the July and August sowing dates. In these samples uniformly coloured, almost with, ring-free roots were obtained. In our trial varieties from the spring sowing had superior taste. Early sowing is recommended for fresh market sale while the second crop (July) harvested in autumn can satisfy processing requirements. In the late sowing (under unheated plastic tent) fresh beet root can be grown at the end of autumn or beginning of winter, thus prolonging the usability of plastic tents.
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Some structural characteristics of the flowers of apple cultivars with different susceptibilty to fire blight
21-23.Views:128Several morphological characters of the hypanthium (size and form of the surface, the shape of the hypanthium) and anatomical traits (number and pattern of stomata) of apple cultivars (Malus domestica L.) with different susceptibility to fire blight were studied. The size of hypantium surface was calculated by modelling the hypanthium with a truncated cone. Three types of hypanthium surface form have been revealed: straight, convex and a complex "shouldered" one. The angle between the style and the wall of the hypanthium was narrow or wide. The stomata on the hypanthium surface can be arranged in a zone in the middle third of the hypanthium or dispersed more or less evenly. The number of stomata/flower substantially differed among the cultivars examined. The highest stomata number was detected in the flowers of the tolerant cultivar (Freedom)
No single characteristics of the hypanthium could convincingly be correlated with susceptibility to fire blight. We suggest, however, that combination of morphological properties that sustain moist environment in the hypanthium contribute to susceptibility.
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Evaluation of morphological parameters and bioactive compounds in different varieties of beetroot (Beta vulgaris L. ssp. esculenta GURKE var. rubra L.)
31-35.Views:466Beetroot consumption based on pickled beets generally in Hungary which is due to the higher yield from second crop harvested in autumn and processed by manufacturing industry. Researches of the past years confirmed its favourable nutritional-physiological effects on human body so demands, as for fresh salad, also increased. The trial aimed at testing the interaction of varieties on quality parameters and in the same time suggestions are made how to use different varieties of beetroot according to its quality. Morphological and sensory evaluations were examined on 10 varieties of beetroot harvested in autumn. The regular spherical shape can reduce the refining loss during the processing of beetroot which is beneficial for the manufacturing industry. In our trial the root shape of Libero, Mona Lisa and Rubin varieties approached most the regular spherical shape (diameter/length – 1.0) which is favoured by not only processing industry but also fresh market. The highest red pigment content (betanin) was observed in Mona Lisa, Akela and Cylindra (34.58–47.66 mg/100 g). A similar trend could be observed in yellow pigments (vulgaxanthins) which proves the close correlation between the quantities of the two pigments (r=0.898). Highest total polyphenol (77.13–83.37 mg GAE/100g) and flavonoid (21.73–22.73 mg CE/100g) contents were detected in Akela, Mona Lisa and Bonel. These varieties are favourable for fresh salad and they can satisfy processing requirements also. Highest water soluble solids content was found in Akela (7.15%). In our conditions nitrate (NO3-N) values below 900 mg/kg were examined in all of the varieties which is favourable in the case of beetroot.
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Impact of substrate supplemented with CaCO3 on mycelial growth, yield, morphological features and storability of fruiting bodies of black poplar mushroom Agrocybe cylindracea (DC.) Marie.
76-86.Views:538Black poplar mushroom, Agrocybe cylindracea deserves special attention, due to its medicinal properties. Water and alcohol extracts from fruiting bodies of the fungus have an anti-oxidant, anti-cancer, anti-fungal, cholesterol and triglycerides blood level lowering abilities. This mushroom is rich in proteins and vitamins, mineral elements and low in fat. The aim of the experiments was to determine effect of a substrate supplementation with CaCO3 on mycelial growth, yield, morphological features and storability of fruiting bodies of four strains of A. cylindracea (DC.) Marie. The amount of additive to sawdust substrate affects rate of mycelial growth and yield of investigated strains. A. cylindracea mycelial growth was not affected by addition of CaCO3 to substrate, however a significant effect of this additive was found on yield, which was the highest with CaCO3 addition in an amount of 8 g/100 g of substrate. Carpophores characterized with the largest caps diameter, and the largest individual mass obtained of substrate enriched with CaCO3 addition of 8 g/100 g of substrate. In addition, it was found that supplementation with CaCO3 affect storability of A. cylindracea. The lowest weight loss of fruiting bodies after 3 and 7 days of storage was found with addition of CaCO3 to substrate in an amount of 4 g/100 g of substrate.
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Comparative analysis of Hungarian Matricaria recutita (L.) Rausch. populations
81-85.Views:226Matricaria recutita L. is a traditional medicinal plant in Hungary and its drug is known as „Hungaricum", world-wide. Plant samples and seeds were collected from 12 different habitats of three significant geographical regions of Hungary in 2001. Morphological, production biological and chemical properties of samples were examined. In relation to the morphological characteristics, a negative correlation (r= -0.75) could be observed between the average height of the plants (height of flowering shoots) and the pH value of the soil. According to the composition of the essential oil, the populations accumulating typically chamasulene (10-20%), a-bisabolol (30-50%) or bisabolol-oxid (30-50%) could be completely distinguished. Concerning the flavonoid composition the quantity of apigenin-7-glucoside was outstanding in the populations originating from the Great Hungarian Plain, it has reached the concentration of 1.8-2.8 mg/g, while the samples collected in Transdanubia could be characterised by much lower level of apigenin-7-glucoside (around 1.5 mg/g).
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Morphological characterization of shallot (Allium cepa L. var. aggregatum) segregating populations obtained from natural-outcrossing in Ethiopial-outcrossing in Ethiopia
74-83.Views:70Shallot is a vegetable and condiment crop widely used in Ethiopia and globally. However, absence of improved and adaptable varieties has been the major cause of low productivity. Narrow genetic base of local shallot germplasm owing to vegetative reproduction of the crop, among others, has been the root cause of low productivity. Nevertheless, some plants within the germplasm were observed bolting and producing viable seeds, presenting an opportunity for genetic diversification. Consequently, a germplasm enhancement program was initiated using these naturally outcrossing genotypes where about eighty-one genotypes were generated. The present study was thus undertaken with the objective of characterizing, classifying, and selecting the eighty-one genotypes for future breeding activities. The genotypes were planted in 9x9 simple lattice design with two replications at Debre Zeit Agricultural Research Center (Ethiopia) during the dry (irrigated) season of 2021. The genotypes were evaluated for fifteen growth, yield, and quality traits. Significant variations were observed among the genotypes in terms of bulb yield, bulb height and diameter, total soluble solids, bolting percentage, and bulb skin color. Bulb yield of the genotypes ranged from 31.33 t/ha in DZSHT-79-1A to 9.63 t//ha in DZSHT-45-1A-1. DZSHT-51-2 (207.93 g) was the highest yielder per plant whereas DZSHT-065-6/90 (74.51 g) was the lowest yielder. DZSHT-14-2-1/90 had the thickest bulb (44.69 mm) significantly thicker than twenty two genotypes which had bulb diameter ranging from 28.92 mm to 20.29 mm. DZSHT-81-1/90 was a genotype with the longest bulb height (52.33 mm) while DZSHT-147-1C was a genotype with the shortest bulb (33.12 mm). DZSHT-307-1/90 had the highest TSS (16.78°Brix) significantly differing from DZSHT-002/07 which had the lowest TSS (11.17 °Brix). Dry matter of the genotypes ranged from 12.00% to 22.79%. DZSHT-004/07, DZSHT-111-2-1, DZSHT-41-2B and DZSHT-72-2 had DM% greater than 20% which coupled with greater than 14 °Brix could make them suitable for dehydrated shallots. Among the 81 genotypes characterized 4 (4.9%), 7 (8.6%), 13 (16.1%), 28 (34.6%) and 29 (35.8%) were yellow, golden, light red, red and dark red in colour, respectively. Fifteen of the genotypes had at least 50% bolting plants whereas twenty nine of the genotypes had less than 25% bolting. The results revealed that seven principal components explained approximately 76% of the observed variation. Cluster analysis grouped the genotypes into seven clusters, with the majority falling into three clusters. The study successfully identified genotypes with diverse and important traits and availed both the genotypes and the information for future breeding programs. These genotypes could be used for the development of improved hybrid and open pollinated shallot varieties with higher yield, quality and pest resistance/tolerance attributes.
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Evaluation of vermicompost application and stress of dehydration on mullein medicinal plants
69-77.Views:172The use of organic fertilizers is one of the suitable solutions in the organic production of medicinal plants due to its good effect in improving soil properties, reducing environmental effects, and better plant growth. To investigate the effect of vermicompost organic fertilizer application and water stress on some morphological and physiological traits of the Mullein medicinal plant, research was conducted at Isfahan Azad University in the form of split plots in the form of a randomized complete block design with 4 replications. The test factors included the application of vermicompost organic fertilizer at three levels of 0, 4, and 8 kg per square meter of soil and water stress at two levels of normal irrigation and irrigation at the time of 50% of the soil's agricultural capacity as the main treatment in research farm conditions. The results of the experiment showed that the application of vermicompost organic fertilizer and water stress improved the morphological and physiological characteristics of the Mullein medicinal plant compared to the control, i.e. no application of organic fertilizers. The results showed that the highest number of secondary branches, number of flowers of the secondary stem, diameter of flowering stem, the diameter of flower, and fresh weight of shoot in the Mullein medicinal plant were obtained by applying vermicompost organic fertilizer at the rate of 4 and 8 kg of soil. The application of vermicompost organic fertilizer at the rate of 8 kg in the soil increased flavonoids compared to the non-use of vermicompost treatment. However, to improve the vegetative growth and increase the reproductive efficiency of the Mullein plant and reduce production costs, the use of vermicompost organic fertilizer is recommended at the rate of 4 and 8 kg of soil, respectively.
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Obtention of new ornamental leaf variants of giant reed (Arundo donax L.) originated from somatic embryogenesis and their photosynthetic parameters
18-24.Views:458Giant reed (Arundo donax L.) is a perennial rhizomatous herbaceous plant, it has been widespread all over the world by human activities. It is a 2-8 meter high, polyploid, sterile species (not produce viable seeds), it can be propagated only by vegetative methods (rhizomes, stem cuttings, in vitro biotechnological methods). It has considered promising dedicated energy crop thanks to high biomass production (20-40 dry tons per hectare depending on microclimate), adaptability of different kind of soils and environment and low energy input required for its cultivation. It has been utilized for energetic purposes, biogas/bioethanol production, cellulose/paper production and ornamental purposes. The objectives of the present study were to determine morphological properties of new ornamental giant reed leaf variants originated from somatic embryogenesis and to evaluate their photosynthetic pigment content and photosynthetic activities. The most typical changes was the appearance of different colour (white, yellow, light green) longitudinal stripes on leaves (also on petiole and on leaf blade). It was significant differences (p<0.05) between green and leaf variants in case of photosynthetic pigments content and photosynthetic activity (Fv/Fm value). There was no detectable chlorophyll a or b content in the white bands of leaves and albino shoots. Total chlorophyll content of the white striped leaf variety was more than twice than the light green leaves. Photosynthetic activity and content of photosynthetic pigments also confirm and determine the morphological characteristics and growth habit of leaf variants.
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Morphological and phenological properties of sour cherry varieties grown in Hungary and their inter-incompatibility relations
114-117.Views:221Regular observations and experiments were performed during a 14 year period on 6 sour cherry varieties. The morphological traits of leaves and fruits were compared, and the phenology of blooming as well as of ripening dates served to start an estimation of the possibilities of mutual pollination and the planning of harvest operations. Experiments involved obligate autogamy, artificially controlled allogamy and open pollination in order to reveal self-fertility, self-sterility or inter-incompatibility relations.
The varietal characters represent, each, different values in the distinction of the items, because of their intra-varietal variability. From that point of view, the most reliable are the data of blooming and ripening time, fruit size and the fertility relations.
Inter-incompatibility was observed between the group of self-fertile, "Pándy type" varieties (`Újfehértói fürtös’, ‘Debreceni bőtermő’, ‘Kántorjánosi’) on one side and the selection of Pándy 7', a self-sterile variety on the other side. Unilateral incompatibility has been detected within the former group of new, self-fertile varieties, the combinations: (`Újfehértói fürtös’ x ‘Debreceni bőtermő’ as well as `Újfehértói fürtös’ x Kántorjánosi’.
Our results prove the close kinship between those three new varieties and the original Pándy variety on the base of being highly similar in their morphology and also of the fact of their inter-incompatibility, though unilateral.
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Phenological and fl ower morphological studies on different plum and prune cultivars
43-52.Views:25320–22 and 5–7 years of kind collection of 100 cultivars studied phenology and fl oral morphological basis of characteristics in 1994–1998 and 2009–2011. The varieties were collected 3–5 trees in the gene garden, all were on C. 359 myrobalan seedling. Detecting differences between the test cultivars are suitable for stamps, and also show that relatively short-term observations are useful for describing the comparison, to distinguish varieties. The taxonomic ordination of cultivars and groups of fruits suitable to distinguish colors shown that the fl owering and ripening time, the nature of the reproductive organs and even Sharka-rate sensitivity is associated with those groups. Differences between the age of trees mainly sexual organs and fertility were shown. Affecting rate of climatic effects was not as large as the first line of erratic rainfall, air temperature spikes or possible values would be expected. The results of the breed, a gene bank of perception, in particular, the localization of hybrids, clone-type variants are useful in the analysis. Among other things – this is due to late-maturing, relatively well-stored temporarily, and not-blue plums, Sharka-infected tolerant or resistant varieties selection (cf. SURÁNYI 2013a-c) and will be disseminate in Hungary.
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Effect of hydroponic and peat-free media in transplant production of Rudbeckia hirta varieties under different photoperiodic lighting and their photosynthetic parameters
110-117Views:403The purpose of this research was to determine the effects of varieties, different light conditions (short day, long day, natural short day with light pollution), and different growing media (perlite, peat-free, peat-based, aeroponics system) on Rudbeckia hirta plant production under controlled conditions (greenhouse). The morphological effects of each treatment (photoperiodic lightings and media) on different Rudbeckia varieties determined at 11 weeks-old ’Napfény’, ’Toto Gold’, ’Autumn Colors’, ’Prairie Sun’ and 16 weeks-old ’Napfény’. Plantlets received 12 hours daylight did not initiate flowers, remained stage of the leaf rosette in case of all varieties. The 14 hours light treatment in the aeroponics system and the same treatment in perlite and control (natural short day with 14 hours light pollution) plantlets had developed inflorescences or flower buds. The inflorescence axis of ‘Napfény’ was appeared at 13 weeks under long-day conditions, with 1.7 (perlite) - 2.7 (aeroponics) flower buds in 16 weeks. ’Toto Gold’, ’Autumn Colors’, ’Prairie Sun’ varieties developed inflorescences at 8 weeks, 14 hours aeroponics system resulted in the most of flower buds (’Toto Gold’: 6.5, ’Autumn Colors’: 3.25,’Prairie Sun’: 4.8 flower buds) at 11 weeks. Long daylight manipulation could be minimized crop times and achieved flowering potted plants at 11 weeks. The peat-based and peat-free media effect was observed on ‘Autumn Colors’. The number of leaves of peat-free ‘Autumn Colors’ transplants (16.8-20.3) was significantly higher than peat-based media (13.5-15.5). Other morphological parameters were not affected by the media treatments.
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Evaluation of Colour Versions of Wild Sage (Salvia nemorosa L.)
111-115.Views:216In the continental weather zone, more and more frequently occurring extreme conditions require continuous renewal of the market which generates constant challenge for the ornamental plant breeders. Most of the traditionally used decorative ornamental plants are sensitive to these extreme conditions. In 2001, Department of Plant Biotechnology, Debrecen University initiated an interdisciplinary breeding program in collaborations with Zoltan Kovats (he dealt with hungarian drought-tolerant plant species) to produce new or reintroduce forgotten drought-tolerant ornamental species into public parks and roadsides. From ~900 species of Salvia genus, Salvia nemorosa L. has been known as a medical plant, however, because of its high adaptation ability and decorative nature it is a highly recommended ornamental plant as well. Salvia nemorosa L. has a low maintenance, extremely droughttolerant, fast growing plant, generates proper cover, and highly competing weeds on roadsides. Nowadays, 50-60 varieties are available; however this number could be increased by new hybrids. Great morphological and colour variation could be seen within the species, from different white to deep violet. The main goal of this research is the production of elite lines with wide colour and morphological variation in wild sage. We have already obtained 25 different clones for further investigation without eliminating the original plants generating an in vitro gene bank as it has been done by Italian breeders.
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Comparative analysis of sour cherry cultivars on their ecological and biological indicators
7-28.Views:362Sour cherries developed in the northern hemisphere, an alloploid hybrid of dwarf sour cherries (Prunus fruticosa) and bird cherries (P. avium), born in the confluence of the two species. However, the ecological and, above all, cold tolerance of the ancestor of cultivated sour cherries is higher than that of wild cherries (De Candolle, 1894; Rehder, 1954; Terpó, 1974; Iezzoni et al., 1991; Faust & Surányi, 1997). The cultivation limits are in the northern hemisphere 38-44. degree. The Carpathian Basin, the Balkans and Asia Minor are considered to be the main birthplaces for sour cherries. The genetic and morphological diversity of sour cherries is greater than that of the basic species (Iezzoni et al. 1991; Faust & Surányi, 1997). In the study, 472 sour cherry cultivars were compared based on 7 relative ecological indicators and 3 biological values. Compared to other Prunus species, we mostly found less variability in sour cherries - not counting their salt tolerance (SB). The partial similarity between open pollination (OP), frost tolerance (FR) and disease resistance (DR) - partly true in terms of varieties, but also reflected the effects of purposeful breeding and selection. The cultivars together - in comparison, showed balance, but in the highlighting, the differences of the 3 cultivar groups became significant. Indeed, the differences between the species of the former Hungarian cultural flora are clearly different (Surányi, 2004), which is also the case when comparing a large number of apricot (Surányi, 2014), plum (Surányi, 2015) and peach (Surányi, 2020) varieties.
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Inter-incompatibility of self- incompatible apricots and their varietal properties
79-81.Views:343There are four apricot varieties grown in Hungary derived from local selections known to bear fruits of giant (60 - 100 g) size: Ceglédi óriás, Nagykőrösi óriás, Szegedi mammut and Ligeti óriás. Being morphologically similar, they seem to be closely related to each other. The detailed study of the morphology (of leaves and fruits) and phenology (of blooming and ripening dates) as well as the fertility relations was aimed to find out the degree of kinship between the varieties in question.
It was stated that the value of morphological traits is variable from the taxonomic point of view. The most important signs of common origin were the time of blooming and the leaf size. Less valuable are the date of m:iurity and the size of fruit because of their variability. In the literature Satin') & Nyeki (1991) published the first proof of inter-incompatible relation between apricot varieties. This should be considered as an argument of close genetic relation between those "giant" varieties of apricots.
The first three varieties. Ceglédi óriás, Nagykőrösi óriás and Szegedi mammut are closer related in blooming and ripening date, as well as in size of fruit to each other than the variety Ligeti óriás.
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Comparative study of different fertile groups in plums
71-76.Views:183The plum traditional fruit species in Hungary, several local cultivars was born in the different grower's districts. The author that investigated, that are morphological differences between an odds find self-fertile, self sterile (with functional stamens) and male sterile plum cultivars. For it thought about main questions of a scientific debate sown up and the study this way gave reactions totalize. There were in three fertile groups 8-8 type of feature plum cultivars in periods of 1992-2001, respectively 1993-1999. It was founded by author big odds found the troops on the basis of 9 traits between. Pistil length of self sterile cultivars very typical, such as sesquipedalian flower peduncle of the self-fertile plums and the hypoandry of male sterile cultivars. The relative stamen number and the pollen viability as well significant odds gave.
The average fruit mass and sharka infection of self-fertile plums this troops extreme work. According to cultivar's averages the shark symptoms standard the right correlation the singular traits, but those one part of her with each other not shown connection.
The annuity potencies underdeveloped the troops behind and the troops within cultivar's differences had case significance. The results usable the male sterile cultivars and progeny further its investments.
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The importance of clonal selection of grapevine and the role of selected clones in production of healthy propagating stocks
15-24.Views:358Genetical alterations and phytosanitary status promote the variability and modify the appearance of vine. Old vine varieties in old vineyards are highly variable and well adapted to selection. Clonal selektion is based on a visual performance: valuable individuals (clones) are picked out according to visible symptoms or characters. The genetical stability of clones is proved by testing the vegetatively propagated progenies on the basis of morphological and molekular (SSR, AFLP, SMPL, RAPD) markes. Authors take great care of the visual phytosanitary selection as part of the clonal selection being the oreliminary step to develop pathogen-free propagation stocks. In Serbia (Vojvodina) the selection breeding has been carried on for several decades resulted in comparative clone trials with home and imported clones of Welsch Riesling, Chardonnay, Pinot gris, White Riesling. Among the clones of home selection SK.54 Welsch Riesling clone is the most valuable. Its clearing from pathogene is being carried on in an interregional IPA programme (HUSRB/0901/214/123) in Kecskemét. In Kecskemét, the centre of the Hungarian Danube vine region 5 vine clones have been registered (Cegléd szépe K.73, Irsai Olivér K.11, Kövidinka K.8, Hárslevelû K.9, Pannónia kincse K.56). Besides them 18 virus-tested clones have also been qualified.Works aiming at their complete exemption are going on in order to obtain clones free of propagation wood-borne diseases.
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The effect of different cytokinins on chlorophyll content and morphological features of in vitro Nidularium ’Kertész Jubileum’
47-51.Views:258During in vitro multiplication of Nidularium ‘Kertész Jubileum’, 20 g/l sucrose, 5 g/l agar, 100 mg/l inositol, and different concentrations of benzyladenine (BA), benzyladenine-riboside (BAR), kinetin (KIN), meta-topolin (mT) were added to the MKC (Knudson, 1946) basal medium. Furthermore, 0.1 mg/l naphthaleneacetic acid was used to every medium. Number of shoots, length of leaves, number and length of roots, chlorophyll (a+b) content were examined and evaluated with Ropstat statistical software. As compared to the other cytokinin, significantly most shoots were obtained in the case of applying BA. Increasing of BA-concentration (as far as 2 mg/l) enhanced shoot number (from 10.92 to 19.26) but 4 mg/l BA resulted only 6.63 shoot. The less efficient cytokinin was KIN, in most cases no more than about 2 shoot was achieved. Regarding the length of leaves, the higher level of BA effected averagely the shorter leaves (from 24,46 to 7.31 mm). KIN effected significantly the longest leaves (43.4-61.29) in inverse proportion to the concentration. The same cytokinin resulted the most (and the longest) roots with the highest rooting percentages, but more KIN decreased the number and length of roots (from 7.95 to 4.4 and from 38.49 to 22.73 mm). There were no definite correlation between cytokinin concentration and chlorophyll (a+b) content, but the highest doses resulted decreasing (except of meta-topolin which leads to the lowest values). Summarizing, BAR effected the highest contents (mostly more than 1400 μg/g), particularly in the case of 1 mg/l (1807.3 μg/g).
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Morphological examination of Hungarian apricot rootstock varieties
59-63.Views:273Apricot seedlings are one of the most widely used rootstocks in apricot growing of Hungary. The National List of Hungary contains 7 apricot seedling rootstocks varieties. The production of apricot graft on seedling rootstock takes 3 years from the seed production to the complete graft and requires more space. It is important to provide the trueness to variety name during this process. In the genus Prunoideae not only the plant but also the stone should have typical characteristics. It seems to be useful to examine the morphology of stone. The observed varieties were 'Tengeribarack C.1300', 'Tengeribarack C.1301', 'Tengeribarack C.1650', 'Tengeribarack' C.1652', 'Tengeribarack C.145', 'Tengeribarack C.1426' and 'Tengeribarack C.2546`.1t seems to be the most suitable characteristic to make a distinction between these varieties by stone, the ratio height of stone to lateral width of stone, the ratio of height of stone to ventral width of stone, the ratio of lateral width of stone to ventral width of stone and the ratio of ventral zone to ventral width. From not measured characteristic seems to be most utilizable to make distinction: the fusion of dorsal groove margins, the shape of apex, the presence of a mucro of the apex, the shape of stalk end in lateral view and the texture of lateral surfaces.
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Book review
37-39.Views:193Plum is a significant temperate fruit and a very important fruit species in Hungary as well. Cultivation has moved beyond the area boundaries of the Northern Hemisphere many centuries ago. Domestic (European) plum production has been particularly affected by the pandemic-scale destruction of the Sharka virus and worldwide breed changes. According to FAOSTAT (2016) data, world plum production is 12 million tones, with 36% from Eurasian, 63% from Japan and other Asian varieties. The share of American plums is only 1%. Domestication and dissemination of plums is „multi-stepped” because homemade (taste) plums are hybrids of two nature species in the first place, but Japanese plums (hybrids with Chinese plums or Prunus cerasifera) are not uniform; the role of the American plum species is much smaller, though their prospects cannot to predict with certainly. The book consists of 19 chapters, finding a complex way of summing up linguistic, historical, floristic, historical-botanical, cultivation, and morphological and anatomical knowledge.
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The impacts of different habitats on the development of Telekia speiosa (Schreb.) Baumg.
31-32.Views:181Telekia speiosa (Schreb.) Baumg. is a 100-150 cm high bushy perennial, which has yellow flowers and smells good. According to the descriptions (Farkas, 1999), it can be detected in two smaller areas within Hungary, namely in the Bükk hills and on the Szatmár-Bereg Plain. By the time of writing this paper, the population around Tiszabecs has already got extinct. Therefore, it is a protected relict species. It is named in honour of Sámuel Teleki, chancellor of Transylvania.Within the frame of the experiment, the Telekia speciosa (Schreb.) Baumg. was planted to places differently illuminated (sunny, semi shadow, shade), then the morphological changes brought about the various light conditions were investigated. The experiment was launched with a stock sown in October 2008. The seedlings were planted to three beds with diverse light conditions. The area of each bed was 1 m2, and ten seedlings were planted per m2. The parameters investigated are as follows: the length of leaf blade, the width of leaf blade, the length of petiole, the number of leaves per plant, and the alterations of leaves. As a result of our research, we can state that semi shadow is the optimal habitat for the plant. Under such ecological conditions the highest leaf production was observed, the leaves were species specific, healthy and big. The mean number of leaves per plant was 6.6, the mean length of blade was 16.6 cm, the mean width of blade was 13 cm, while the mean length of petiole was 14.2 cm. In the shade the plants grew poorly and the size of leaves were smaller. The mean number of leaves per plant was 4.1, the mean length of blade was 8.6 cm, the mean width of blade was 7.1 cm, and the mean length of petiole was 9.4 cm. In the sunny habitat a similarly high leaf production was observed as in the semi shadow; however, the leaves had brownish spots, they shriveled, feel rough, so they revealed a reduced aesthetical value. The mean number of leaves per plant was 6.6, the mean length of blade was 17.8 cm, the mean width of blade was 11.3 cm, and the mean length of petiole was 13.1 cm.
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Introduction of alkanet (Alkanna tinctoria (L.) Tausch), a traditional dye plant into cultivation
41-46.Views:339As a part of the research project to establish natural sources of plant pigments, possibilities of introduction of Alkanna tinctoria (alkanet) into cultivation were studied.
As a result of the germination experiment, the relevance of 21 days' duration of germination procedure was proved. To get high germination rate alkanet fruits are proposed to pretreat by gibberellinic acid (GA3) in the concentration of 400 ppm, overnight before sowing. This method results approximately 50 % germination rate.
The morphological and production properties of alkanet roots are characterized during ontogenesis. Transplanted populations can be characterized by numerous, thick and heavy roots comparing to the spontaneous ones. Thus, seed sowing and transplantation proved to be an effective method for cultivation of the species.
According to our results it can be concluded that in cultivation the optimal harvesting time of roots is at the end of the second vegetation cycle, when the dry root mass of the individuals is about 10-20 g with 3,0-3,5 % accumulation level of active substances.
Considerable seasonal variability have been found influencing not only the root masses, but also the accumulation levels of alkannin derivatives. In a more humid vegetation cycle the root size and mass as well as the content of active substances are much higher.
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The after-effect of paclobutrazol on morphological characteristics of in vitro Narcissus poeticus ssp. radiiflorus plants
43-46.Views:197After different pre-culturing period (12, 23 or 34 days) on ½ MS medium with 1 mg l-1 paclobutrazol, 1 mg l-1 N6-benzyladenine and 0.1 mg l-1 1- naphthaleneacetic acid , 3 groups of Narcissus poeticus ssp. radiiflorus bulb scales were kept on the same medium without hormones. The results were evaluated monthly and the final one happened after 7 month. The best results were achieved due to the shortest pre-culturing period (12 days; Group 1), with 4.9 bulblets and 4.54% hyperhydricity. The result of the second treatment (pre-culturing period of 23 days; Group 2) was not different significantly but the number of bigger bulblet were higher (4.54 bulblets). After the longest pre-culturing period (34 days; Group 3), the number of bulblets was low (3.68) and more hyperhydricity (18.18%) was detected. The highest number of roots (13.91) was observed in this group
very likely due to the strong after-effect of paclobutrazol. -
Molecular characterization of apricot (Prunus armeniaca L.) cultivars using cross species SSR amplification with peach primers
53-57.Views:241Apricot takes an important place in Hungarian fruit production. Considering morphological characteristics of apricots it was concluded that the genetics background of European cultivars is very limited. Molecular markers and their use for genotyping have revolutionized the identification of cultivars. In a classic apricot breeding program, it is important to be able to establish unique DNA profiles of selections to identify them unambiguously and to determine their genetic relationship. Presently SSR is far the most frequently performed technique for genetic diversity studies. In this study there were used peach and apricot primer pairs from four different sources in order to examine microsatellite polymorphism among cultivars and investigate relationships among them. The possibility of cross species amplification among different Prunus species using SSR primers allowed us to use primers developed in peach to study genetic diversity in apricot. In this work, 90% of the primers used were able to amplify SSRs in apricot and more than half of them were polymorphic. With the 10 primer pairs utilized were proven to be sufficient to set unique fingerprint for several cultivars studied. The obtained dendrogram classified of the 45 cultivars included in this study into two major groups and several subgroups.