Search
Search Results
-
Effect of dry and wet years on primary inoculum source, incubation period and conidial production of Venturia inaequalis
53-58.Views:202The present study focuses on the characteristics of epidemics caused by the selected model-pathogen, Venturia inaequalis in relation to weather elements, with special respect to the increasing or decreasing effects of the specific weather elements. First, those weather elements are discussed which have a role in the development of scab epidemics. Subsequently, by accepting the thesis that climate change includes also weather extremes, an extremely hot and dry year (2003) and a colder year of higher than average precipitation (2004) were chosen as models. The presented examples verified that the variability of weather elements had had an undoubtable effect on the development of epidemics. The variability of weather elements manifests in the unusual behaviour of the pathogen, resulting in no or extreme disease epidemics. The extremities are well demonstrated by the fact, that in a year of drought an efficient protection can be achieved by considerably less applications than average, while in the next rainy year, the susceptible cultivar cannot be protected effectively even with such a high number of applications as is usual under humid Western-European climate conditions. It can also be noted, that the pathogen has a very good adaptability under unfavourable weather conditions. Consequently, more efficient management strategies should be developed for protection against the effects of extremities. However, it should be emphasized that it is very difficult to adapt to the variability and extremities of weather in the practice, because no long-term, accurate and reliable information is available about the variability of these elements.
-
Studies on some seed traits of Iris pumila L., Adonis vernalis L., Primula veris Huds. and Alkanna tinctoria (L.) Tausch.
36-40.Views:130In this study we summarize the results of a five-years period concerning seed traits examinations on Iris pumila, Adonis vernalis, Primula veris and Alkanna tinctoria, with special attention to seed dimensions, seed mass and other traits concerning plant fitness, to their variability and the relationship among them. We found tight correlation between seed weight and seed dimensions in Adonis and Primula, at the same time no correlation exists among the same characters in Alkanna tinctoria. Consequently, the seed weight and seed dimensions can be used as synonyms in the form of „seed size" only after preliminary detection of correlations among them.
The variability of seed traits is higher in natural categories (individuals, morphs) than in seed mass categories as speculative groups. When we need homogeneous plant stand (e.g. for an introduction experiment) it is suggested to use seeds pre-selected in this way. For ex situ conservation, where the central goal is to maintain the genetic variability, seeds originated from different individuals are preferred.
-
Improvement of the grapevine variety ‘Kadarka’ by the selection of new clones
57-63.Views:174Red wine culture in Hungary was developed due to the grapevine variety ‘Kadarka’, originated from the Balkans, from the 16th and 17th centuries. ‘Kadarka’ was the first grapevine variety cultivated on the largest area in Hungary until the 1950’s, but its production area has been reduced significantly in the past decades. In the period of Hungarian grapevine reconstruction (from the 1960’s), the selection of the grapevine varieties and clones was determined by the system of cultivation (large growing space, large load and mechanized technology) that was based on the aspects of quantity. It did not promote the majority of the autochton and regional grapevine varieties to remain competitive. ‘Kadarka’ is a grapevine variety having an extraordinary variability in forms; however, a considerable part of its old stocks has disappeared. Nowadays, only two of its high yield clones are cultivated. ‘Kadarka’ becomes more differentiated from other, red wine producing, grapevine varieties, more preferred and newly requested by the fine aroma, spice, unique harmony of its wine less rich in tannin. The new market and environmental challenges in the field of wine production necessitate the preservation and improvement of the biological basis. In 2001 the Institute of Viticulture and Enology Pécs analyzed an old stock having a variability in forms in order to preserve the development and variability of the biological basis of ‘Kadarka’. Clones of great biological value were selected from it, with which the quality and yield security of ‘Kadarka’ can be increased. In 2010 and in 2012, six clones were submitted for state approval.
-
Variability and differences of growth vigour in the set of 36 genotypes of apricot (Prunus armeniaca L.).
30-34.Views:129Growth vigour of 36 apricot cultivars and new hybrids grafted on apricot seedling rootstock (Prunus armeniaca L.) was evaluated on the base of measurements of stem girth from the 411' to the 10th year after planting. There were differences in growth vigour of genotypes under study. In the evaluated set of genotypes the control cultivar 'Veecor may be classified as a genotype with below-average growth vigour. Only four genotypes (-Reale d'Imola-, Sanagian -Moldavskii krupnoplodnyl and 'LE-2385') were found with significantly higher growth vigour than that of control cultivar 'Veecot' in years of the end of experimental period. Two genotypes (Farmingdale', -LE-SE0-24') were found with significantly higher growth vigour only at the beginning of experimental period and one cultivar ('Vivagold-) with significantly lower growth vigour in the first four years. Genotypes with different growth vigour can be used in further breeding programmes and/or as components inhibiting or supporting the growth in indirect vegetative propagation. Within the whole experimental period, the rank of growth vigour of genotypes practically did not change. This was demonstrated by highly significant or significant coefficients of correlation existing between individual pairs of years (r=0.32+ to r=0.96++). As far as the time difference between years in individual pairs of years was higher, the correlation coefficients were lower. In individual years, variability of growth vigour was relatively low and ranged from 9.83 to 13.64%.
-
Evaluation of potential of fruit weight and fruit width and their interrelationships in the set of apricot genotypes
47-53.Views:106Within the period of 1994-1999, variability of fruit weight and width was evaluated in altogether 24 apricot genotypes. It is concluded that the genotype and climatic conditions of individual years are the factors causing variability in these two parameters of apricot fruits. Variability of fruit weight was significantly higher (approximately 3-times) than that of fruit width. When evaluated on the base of their width, 75.0% and 95.8% of apricots were classified into the groups of extra and first quality, respectively. As genotypes with the maximum fruit width (i. e. above 50 mm) the following cultivars were classified: 'Velkopavlovicka LE-6/2', 'NJA-1', 'M 45', 'M-25' and lednicka (M-90-A)'. The value of fruit width (in mm) corresponded with its weight (in g) only within the range of 40 — 45 mm. With the increasing and/or decreasing size of apricot fruit the changes in fruit weight were more pronounced than in those in fruit width. Within the set of genotypes under study, this relationship may be expressed by the equation y = 0.1234 — 7.6605 + 152.76; the corresponding values of correlation coefficient and coefficient of curve determination are r = 0.95-H- and R2 = 0.959.
-
Development of microsatellite markers for Rhodiola rosea
37-42.Views:217Rhodiola rosea L. is an important adaptogen medicinal plant. In this study two new microsatellite markers were developed. The assessment of the genetic diversity of R. rosea has recently started with molecular markers, but only a few species-specific microsatellite markers have been published so far. However the small number of markers allows only a limited insight into the genetic variability of the species therefore the aim of our work was to develop new microsatellite markers for R. rosea with a microsatellite enrichment library technique. Genomic DNA was cleaved with an endonuclease enzyme followed by adaptor ligation and PCR amplification. DNA fragments that contained microsatellites were first isolated using a biotin-streptavidin linkage based magnetic selection and then cloned into plasmids. Out of forty-three sequenced clones three contained microsatellites, in these cases primers were designed for the amplification of the microsatellite repeats. The newly developed primer pairs were tested on individuals from distant R. rosea populations and the variability of the amplified fragments was estimated by fragment-length analysis. The locus RhpB14a was found to be monomorphic while RhpB14b and RhpB13 were polymorphic. As a result of the present study, two novel variable microsatellite loci were identified in the genome of R. rosea.
-
Comparative investigation on Hypericum perforatum L. populations of different origin
56-60.Views:205Widespread application for herbal medicines based on Hyperici herba has been experienced in the last few years, especially in the treatment of depression syndrome. As the wild origins could not satisfy the market demands neither in quantitative nor in qualitative respect, the necessity of the development of intensive growing methods has been raised. In the course of our investigations we intended to clear up the morphological and chemical variability among and within populations of different origin in order to start a new breeding program. According to our data, growth dynamics of populations could be characterised by a logistic curve. According to the time of flowering the populations formed early, middle and late groups. Morphological diversity among populations was measurable in differences of flower length, plant height, different leaf types and plant habit, according to which characteristics groups were distinguished. Generally, the accessions were the most homogenous in plant height (CV: 7-15%), followed by inflorescence-lengths (CV: 11-36%) and the least uniform characteristics proved to be the number of flowering shoots (CV: 14-59%). The greatest morphological heterogeneity was experienced in the accessions of wild origin as it has been expected. In the second vegetation period generally a much better homogeneity was obtained, than in the first year. The most outstanding accessions produced 1.2 t of dry flowers and 4 t of dry flowering shoots, calculating to one hectare area. The individual yields varied on a large scale in each population (CV: 18-70%).
The content of hypericin varied between 1,85 and 9,9 mg/g in 1996, and between 0,18 and 2,70 mg/g in 1997, showing high individual divergences. Flavonoid values -including first of all hyperosid, rutin and quercitrin - reached 17-39 mg/g in the first and 15-20 mg/g in the second year, respectively. Individual and seasonal variation was less than in hypericin. A joint high level of these two compound groups seems to have low frequencies.
The superior population were selected for further breeding.
-
Molecular identification of old Hungarian apple varieties
37-42.Views:254Altogether 40, mainly old Hungarian apple varieties were screened with six previously described microsatellite markers. A total of 71 polymorphic alleles were detected (average 11.8 alleles/locus) and the heterozygosity of markers averaged very high (0.8). The genetic variability among the genotypes proved to be so remarkable that as few as three markers from the applied six were enough to distinguish between the 40 varieties. This was also confirmed by the cumulative probability of obtaining identical allele patterns for two randomly chosen apple genotypes for all loci, which value was quite low: 2.53 x 10-5. The molecular identification of these genetically very different old apple genotypes could be very useful in future breeding programs.
-
Variability of the data indicating the fertility of different plum varieties
51-55.Views:175Self-fertility and fertility at conditions of open pollination in plum varieties is strictly determined by genetic factors. However, rates of fruit set are highly variable according to growing sites as well as to seasons, which may result from a couple of inner and outer conditions, but mainly from the method applied in seizing the facts of fertility in the experiments planned including the number of replications of treatments. During three successive years, 4-16 trees of each of the four plum varieties have been selected and 16-64 branches were tagged either for checking their fertility as pollinated freely or isolated excluding the access of foreign pollen. The data of fruit set have been processed in order to determine the variability of the data, subsequently, the number of replications necessary to make reliable decisions. Both autogamy and open pollination displayed multiple differences between branches and trees studied.
A number of 20 branches are needed yearly for each variety, the branches should be distributed on 5 trees at least for checking the autogamy, whereas on 10 trees for the results of open pollination. Each variety and treatment should be represented in three seasons, at least because of the different weather conditions.
-
Antioxidant capacity and total polyphenol content of Lavandula cultivars at different growing areas in Hungary
65-69.Views:334Lavandula species are worldwide grown essential oil producing medicinal plants with considerable economic value. Beside volatile oil, lavender also contains different phenolic compounds which have been less widely studied till now. Cultivation of lavender has become widespread in Hungary in the recent years, however, growers have limited knowledge on the productivity of cultivars available. In our research we were aimed to studying the antioxidant capacity and total polyphenol content of samples collected in two growing areas (Dörgicse and Szomód) from flowers of two L. angustifolia (‘Hidcote’, ‘Munstead’) and two L. × intermedia (‘Grosso’, Grappenhall’) varieties, during the full blooming period of 2017. Antioxidant capacity of the samples was determined by FRAP assay (Benzie and Strain, 1996), while total polyphenol content (TPC) was measured by a modified method of Singleton and Rossi (1965). According to our results, varieties of L. × intermedia showed higher values of antioxidant capacity and of total polyphenol content, than those of L. angustifolia cultivars. Among them, both the antioxidant capacity (179.6 mg AAE/g DW) and total polyphenol content values (152.4 mg GAE/g DW) of ’Grosso’ from Dörgicse were the highest. Concerning FRAP values of all the cultivars investigated, larger variability were found in Dörgicse, than in Szomód. Regarding the effect of growing area, each cultivar represented similar FRAP values in Dörgicse and in Szomód, except for ’Grosso’. However, in the case of TPC values, higher variability was observed between the growing areas, especially in the case of ’Grosso’. In the future our studies on lavender polyphenols will be completed with qualitative evaluation of the values obtained by HPLC analysis.
-
Effect of different cultivation methods on strawberry’s antioxidant value
7-10.Views:389The small fruit and strawberry production is economically important in Serbian agriculture. The fresh fruit is sold in domestic markets, but the frozen and processed is mainly sold in foreign markets. The growers find themselves in an ever-expanding selection of the varieties, but besides the nursery and breeder’s descriptions of new cultivars, there are only few exact results for the biological and economical ability of the varieties, which makes easier to choose the proper cultivar. The performance of the varieties in modern strawberry production is worth to investigate by adaptive cultivation methods and production technique. In the case of new strawberry varieties we know neither their needs in production nor their real capability, so they have to be compared with an older, standard control variety (‘Clery’). The breeder’s manuals give only references for the needs of the production technique, of the variety, so it is necessary to have facts, which are based on objective testing results. In this work, a new variety (‘Joly’) is being compared with the control variety. Comparative study of individual varieties are required covering the antioxidant content of the fruits (FRAP) and variability of these amounts in different years of breeding as well as the production techniques under similar circumstances.
-
Morphological and phenological properties of sour cherry varieties grown in Hungary and their inter-incompatibility relations
114-117.Views:175Regular observations and experiments were performed during a 14 year period on 6 sour cherry varieties. The morphological traits of leaves and fruits were compared, and the phenology of blooming as well as of ripening dates served to start an estimation of the possibilities of mutual pollination and the planning of harvest operations. Experiments involved obligate autogamy, artificially controlled allogamy and open pollination in order to reveal self-fertility, self-sterility or inter-incompatibility relations.
The varietal characters represent, each, different values in the distinction of the items, because of their intra-varietal variability. From that point of view, the most reliable are the data of blooming and ripening time, fruit size and the fertility relations.
Inter-incompatibility was observed between the group of self-fertile, "Pándy type" varieties (`Újfehértói fürtös’, ‘Debreceni bőtermő’, ‘Kántorjánosi’) on one side and the selection of Pándy 7', a self-sterile variety on the other side. Unilateral incompatibility has been detected within the former group of new, self-fertile varieties, the combinations: (`Újfehértói fürtös’ x ‘Debreceni bőtermő’ as well as `Újfehértói fürtös’ x Kántorjánosi’.
Our results prove the close kinship between those three new varieties and the original Pándy variety on the base of being highly similar in their morphology and also of the fact of their inter-incompatibility, though unilateral.
-
Simultaneousness of reproductive organs of sour cherry cultivars adaptable to Iranian climatic condition
25-31.Views:226Observations were made on simultaneousness of pollen shedding and stigma viability ratio, to clarify the phenology of reproductive organs of nine economically important sour cherry (Prunus cerasus L.) cultivars (‘Érdi bôtermô‘, ‘Debreceni bőtermő‘, ‘Csengődi‘, ‘Kántorjánosi 3‘, ‘Újfehértói fürtös‘, ‘Petri‘, ‘Eva‘, ‘Oblascinszka‘, and ‘Pándy 279’). The main aim was investigation the possible influence of environmental cues in timing and development of phenophases and also responses of different cultivars to seasonal cues. Likely effects of climatic changes on phenological development patterns were also considered. Results showed important aspects of the reproductive biology of sour cherry flowers. The amplitude of the phenophase “beginning of blossoming” between cultivars did not exceed 6 days. There is a maximum 3 day difference in the blooming length of different directions in each cultivar. ‘Pándy 279‘ showed high variability when the position of flowers changed on the tree shoots. ‘Újfehértói fürtös‘ was stable in all four directions of the tree. The pollen shedding period and stigma viability ratio were synchronous. Pollen shedding phenomenon simultaneously occurred with the secretary activities of stigmas in all examined cultivars. Distribution of pollen shedding over the secretary activity of stigmas is almost good. In all five cultivars maximum pollen shedding occurred when the temperature was high during the day. In general the results of this experiment and our previous studies clearly demonstrate that the Pándy groups not recommended for plantation in new orchards of Iran.
-
Identification of the apple firmness: two case studies
32-35.Views:112Firmness tests were performed with peeled and entire fruits of Elstar and Jonagold apple cultivars for identification and comparison. The normal distribution of the tested population was acceptable (level: 95%). The green and the red sides did not show differences within the cultivar but they were different in firmness. There was not significant difference between the flesh tissue firmness values, however the firmness of the entire (not peeled) fruits was different. This result was caused by the effect of the peel. The variability of the firmness with Jonagold was caused by the peel, but such a result was not found with Elstar. The test of the peel effect would be interesting with different cultivars and a sequence according to the firmness can be estimated.
-
Genetic relatedness among Asian Cotoneaster species investigated with DNA marker analysis
43-46.Views:164The widespread genus Cotoneaster has its centre of diversity in the Himalayas and surrounding areas. Most taxa appear to be polyploid and apomictic, and many of them have become popular ornamentals due to their attractive foliage and berries. One of the taxonomically most critical groups is Section Alpigeni which contains several important ornamental plants. The number of species belonging to this section varies widely between different taxonomic treatises, depending on whether 'splitting' or 'lumping' of species is preferred. Using a rather narrow species definition, we have investigated 13 different species using RAPD analysis. A simple matching (SM) coefficient-based principle coordinate analysis (PCO) was calculated from the RAPD data. Some species were clearly more similar to each other than to other species in the analysis. The levels of similarity did, however, not correspond very well to the lumping together of several taxa under the same species name as performed e.g. in the recent Flora of China. Obviously, the complex hybridogenous origination and, in some cases, still ongoing recombination with sexual species or among the apomictic taxa themselves, produces a genetic variability structure that cannot be properly reflected in a hierarchical taxonomy.
-
Seasonality of weather and phenology of reproductive organs of flower of sour cherry cultivars in Hungarian climatic conditions
75-80.Views:212Sour cherry (Prunus cerasus L.) is one of the most important fruit crop grown in Hungary, The flowering phenology and pollen shedding/stigma viability ratio, in an effort to elucidate the reproductive phenology of nine economically important sour cherry cultivars (Érdi bőtermő, Debreceni bőtermő, Csengődi, Kántorjánosi 3. Pándy 279. Úfehértói fürtös, Petri, Éva, and Oblacsinszka) all widespread in the Hungary, was studied at Újfehertó climatic condition. The main aims were to investigate how possible environmental cues influence timing and development of phenophases and whether different cultivars have different seasonal responses to these cues. Likely effects of climatic changes on phenological development patterns were also considered. Our results revealed important aspects of the reproductive biology of sour cherry flowers. The amplitude of the phenophase "beginning of blossoming" between cultivars did not exceed 6 days. There is maximum 3 days difference in blooming length of different direction in each cultivar. Pándy 279 showed high variability when the position of flowers changed on the tree shoots. Újfehértói fürtös was stable in all four directions of tree. The pollen shedding period and stigma viability ratio was well synchronous. Pollen shedding phenomenon occurred in the range limitation of secretary activity of stigmas in all five cultivars. Distribution of pollen shedding over the secretary activity of stigmas is almost good. In all five cultivars maximum pollen shedding occurred about the high temperature part of the day.
-
Introduction of alkanet (Alkanna tinctoria (L.) Tausch), a traditional dye plant into cultivation
41-46.Views:302As a part of the research project to establish natural sources of plant pigments, possibilities of introduction of Alkanna tinctoria (alkanet) into cultivation were studied.
As a result of the germination experiment, the relevance of 21 days' duration of germination procedure was proved. To get high germination rate alkanet fruits are proposed to pretreat by gibberellinic acid (GA3) in the concentration of 400 ppm, overnight before sowing. This method results approximately 50 % germination rate.
The morphological and production properties of alkanet roots are characterized during ontogenesis. Transplanted populations can be characterized by numerous, thick and heavy roots comparing to the spontaneous ones. Thus, seed sowing and transplantation proved to be an effective method for cultivation of the species.
According to our results it can be concluded that in cultivation the optimal harvesting time of roots is at the end of the second vegetation cycle, when the dry root mass of the individuals is about 10-20 g with 3,0-3,5 % accumulation level of active substances.
Considerable seasonal variability have been found influencing not only the root masses, but also the accumulation levels of alkannin derivatives. In a more humid vegetation cycle the root size and mass as well as the content of active substances are much higher.
-
The antioxidant capacity of sea buckthorn (Hippophae rhamnoides L.) berries depends on the genotype and harvest time
27-29.Views:267Berries of sea buckthorn (Hippophae rhamnoides L.) are characterized by increasing popularity due to their presumable healtheffects. The aim of this study was to compare the antioxidant capacity and total polyphenolic content in the berries of six Hungarian grown sea buckthorn genotypes and characterize the genetic variability in this trait. The harvest time of sea buckthorn berries affects the antioxidant capacity and total phenolic contents in berries of three popular cultivars of German origin. Berries harvested in October had higher antioxidant capacity compared with those harvested one month later. The extent of the difference was genotype-specific. Our analysis revealed a nearly 3-fold difference between the lowest and highest antioxidant capacities of the 6 tested genotypes with ‘Leikora’ showing the highest ferric reducing antioxidant power and total phenolic content. The TEAC values ranged between 1.76 and 3.13 mmol Trolox/100g fresh weight with Pető 1 and ‘Frugana’ having the highest values. The results presented in this study demonstrated that Hippophae rhamnoides berries possess in vitro antioxidant activity strongly determined by genotype but also influenced by harvest time.
-
Evaluation of fruit quality parameters of Rosa taxa from the Carpathian basin
81-87.Views:162From the wide range of genetic sources available in Hungary, we have chosen as objective the evaluation of those rose taxa, which — on the base of their quality and morphologic features — are suitable for selecting fruit production varieties or parent varieties for further breeding. We examined physical and inner parameters of fruits of Rosa taxa, and evaluated the correlations by mathematical statistic methods. Namely, if a correlation can be found between physical and inner parameters, fruit production value can be determined by less examinations in the future. According to our results, there is a large variability in some physical parameters (weight, diameter of fruits; weight, number of achenes) as well as in inner content values (vitamin C, glucose, fructose, K, Ca, Fe, Zn and Cu content) of rosehip, regarding production year, habitat and even the individual of examination. Twice as much ascorbic acid can be found in achene-free fruits, and nearly five times as much in their dry product as in fresh whole fruits. It was established repeatedly, that vitamin C concentrates in fruit flesh, and ascorbic acid content can be preserved better in achene-free flesh. There is no essential difference in citric acid and carbo-hydrate content of whole and achene-free rosehips. However, macro- and microelement content of whole fruits is 30-40% higher than that of achene-free fruit flesh. The connection between fresh weight and diameter, as well as achene number and seed weight of fruits can be described by the function y=axb. A significant relationship can be found in case of K-Fe, Ca-Mg, Ca-B, Ca-Mn and Zn-Cu, between fresh weight and B content of fruits. According to our examinations, fresh weight of rose species with a higher citric acid content is usually higher, while their Fe content is smaller. Glucose content of fruits of rose species is directly proportional to their Ca and Zn contents. Zn content of rose species with higher fructose content is usually smaller.
-
Inter-incompatibility of self- incompatible apricots and their varietal properties
79-81.Views:288There are four apricot varieties grown in Hungary derived from local selections known to bear fruits of giant (60 - 100 g) size: Ceglédi óriás, Nagykőrösi óriás, Szegedi mammut and Ligeti óriás. Being morphologically similar, they seem to be closely related to each other. The detailed study of the morphology (of leaves and fruits) and phenology (of blooming and ripening dates) as well as the fertility relations was aimed to find out the degree of kinship between the varieties in question.
It was stated that the value of morphological traits is variable from the taxonomic point of view. The most important signs of common origin were the time of blooming and the leaf size. Less valuable are the date of m:iurity and the size of fruit because of their variability. In the literature Satin') & Nyeki (1991) published the first proof of inter-incompatible relation between apricot varieties. This should be considered as an argument of close genetic relation between those "giant" varieties of apricots.
The first three varieties. Ceglédi óriás, Nagykőrösi óriás and Szegedi mammut are closer related in blooming and ripening date, as well as in size of fruit to each other than the variety Ligeti óriás.
-
Influence of different growth regulators on the in vitro morphogenesis of an ornamental variety of carnation
55-57.Views:142Callus formation, as a prerequisite for the induction of somaclonal variability, was achieved successfully with certain molar ratios between 2,4-dichlorophenoxyacetic acid and benzyladenine. Regeneration of new plants from shoot apex meristems could be significantly improved by the combined addition of very low amounts of indolebutiric acid, benzyladenine and gibberelic acid, dissolved in the Murashige-Skoog nutrient medium. These in vitro treatments may contribute to a more efficient micropropagation of the Rimini variety of carnation.
-
Comparative analysis of sour cherry cultivars on their ecological and biological indicators
7-28.Views:344Sour cherries developed in the northern hemisphere, an alloploid hybrid of dwarf sour cherries (Prunus fruticosa) and bird cherries (P. avium), born in the confluence of the two species. However, the ecological and, above all, cold tolerance of the ancestor of cultivated sour cherries is higher than that of wild cherries (De Candolle, 1894; Rehder, 1954; Terpó, 1974; Iezzoni et al., 1991; Faust & Surányi, 1997). The cultivation limits are in the northern hemisphere 38-44. degree. The Carpathian Basin, the Balkans and Asia Minor are considered to be the main birthplaces for sour cherries. The genetic and morphological diversity of sour cherries is greater than that of the basic species (Iezzoni et al. 1991; Faust & Surányi, 1997). In the study, 472 sour cherry cultivars were compared based on 7 relative ecological indicators and 3 biological values. Compared to other Prunus species, we mostly found less variability in sour cherries - not counting their salt tolerance (SB). The partial similarity between open pollination (OP), frost tolerance (FR) and disease resistance (DR) - partly true in terms of varieties, but also reflected the effects of purposeful breeding and selection. The cultivars together - in comparison, showed balance, but in the highlighting, the differences of the 3 cultivar groups became significant. Indeed, the differences between the species of the former Hungarian cultural flora are clearly different (Surányi, 2004), which is also the case when comparing a large number of apricot (Surányi, 2014), plum (Surányi, 2015) and peach (Surányi, 2020) varieties.
-
Genetic diversity in a collection of apple (Malus x domestica Borkh.) cultivars as revealed by RAPD markers
25-35.Views:179A collection of 151 apple cultivars was investigated with 7 RAPD primers generating 71 informative bands, to evaluate genetic variability and relatedness. All cultivars presumably derived through genetic recombination were distinguished whereas identical DNA profiles indicated that some cultivars had arisen as sports. A cluster analysis and a PCO did not reveal any distinct geographic patterns, but there was a weak tendency for Swedish and foreign cultivars to differentiate.. Many cultivars however clustered together with either one of their parents or with siblings. Overall genetic diversity among the 151 cultivars was estimated with Nei's diversity index (H), 0.269, and with Shannon's index (H'), 0.594. The cultivars were also analysed in six groups, according to time of origination and country of origin, with an average H = 0.262 and H' = 0.546. No major differences in genetic diversity were observed over time or space, although the group with recent, foreign cultivars had the lowest diversity (FL = 0.235, H' = 0.493). Comparison between the entire material and a subset with 94 mandate cultivars chosen for preservation in Sweden, showed similar genetic diversity: HFN.rj1E = 0.268, frE,NTIRE, = 0.593 and HMANDATE = 0.263, WMANDATE = 0.575. No major differences in band frequencies were observed between these two sets, but 5 RAPD bands were missing in the set with mandate cultivars.
-
Results in the determination of some Hosta varieties by the method of isoelectric focusing
90-92.Views:121Mass propagation of 5 newly introduced Hosta varieties was carried out by the method of micropropagation. Our aim was to determine exact variety specificity after the micropropagation period in the pattern of peroxidase isoenzymes by isoelectric focusing in pH 3-9 range and to determine that phenological phase of mother plant in which the isoenzyme pattern of mother plant can safely be comparable to the isoenzyme pattern of micropropagated descendants. The isoenzyme patterns of descendants were similar to the mother plants of the same hybrid lines. The older leaves seemed to be not so suitable for examination than newly developed ones despite of the higher activity of peroxidase enzymes. There were big differences in isoenzyme patterns of leaves in different phenological phases. With this quick and easy method Hosta varieties could be selected already in the very early stage of micropropagation.
-
The importance of clonal selection of grapevine and the role of selected clones in production of healthy propagating stocks
15-24.Views:318Genetical alterations and phytosanitary status promote the variability and modify the appearance of vine. Old vine varieties in old vineyards are highly variable and well adapted to selection. Clonal selektion is based on a visual performance: valuable individuals (clones) are picked out according to visible symptoms or characters. The genetical stability of clones is proved by testing the vegetatively propagated progenies on the basis of morphological and molekular (SSR, AFLP, SMPL, RAPD) markes. Authors take great care of the visual phytosanitary selection as part of the clonal selection being the oreliminary step to develop pathogen-free propagation stocks. In Serbia (Vojvodina) the selection breeding has been carried on for several decades resulted in comparative clone trials with home and imported clones of Welsch Riesling, Chardonnay, Pinot gris, White Riesling. Among the clones of home selection SK.54 Welsch Riesling clone is the most valuable. Its clearing from pathogene is being carried on in an interregional IPA programme (HUSRB/0901/214/123) in Kecskemét. In Kecskemét, the centre of the Hungarian Danube vine region 5 vine clones have been registered (Cegléd szépe K.73, Irsai Olivér K.11, Kövidinka K.8, Hárslevelû K.9, Pannónia kincse K.56). Besides them 18 virus-tested clones have also been qualified.Works aiming at their complete exemption are going on in order to obtain clones free of propagation wood-borne diseases.