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Application of DNA markers for detection of scab resistant apple cultivars and selections
59-63.Views:108A DNA marker-based study was undertaken to identify the occurrence of major scab resistance genes in some apple cultivars and selections of importance for apple breeding. Unfortunately none of the RAPD-based markers previously reported to detect the Va, Vb, Vr and Vx genes produced unambiguous results. By contrast, the CAPS marker MI8 produced the expected three bands in all cultivars and selections already known or suspected to have the Vf gene, as well as in the Russian cultivar 'Antonovka Polotora Funtovaja' suspected to have Va resistance which however may be allelic to Vf. Vf-carrying selections and newly named cultivars 'Frida' and 'Fredrik' are grown successfully in Sweden without fungicides, suggesting that the Vr resistance breaking scab races 6 and 7 have not yet become a problem. The SCAR marker B12 detected the Vm gene in 'Prairifire', 'Rouville', clones 'OR45T132' and 'OR48T70', and selection '16-36-193'. The SSR locus 0102b10 detected one band at 118 by in 'Reka'. This is presumed to be identical to the Vr gene marker previously reported.
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Agroclimatological properties of growing sites assigned to apple and pear production in Hungary
95-97.Views:191Apple and pear growing sites in Hungary are classified into four regions according to the Hydro-thermic Coefficient: dry, moderately dry. moderately humid and humid. Most of the plantations of apple and pear are located in regions considered as moderately dry and moderately humid. Within that category, the two respective species have different preferences, i.e. the ecological features of Hungary give different opportunities for apple and pear growing. Apple is grown almost everywhere in the country, successfully. The selection of cultivar-regions is needed mainly for increasing competitiveness on the market. Main apple growing regions are listed in 3 large groups. For the definition of cultivar-regions, mainly the configurations of soil and precipitation, i.e. conditions of the soil and opportunities of gaining water were decisive. Market factors are also considered. The area assigned to pear is much less than that of apple, in Hungary. Some well known and popular varieties would require high air humidity which cannot be presented in most of Hungary. Therefore, the possibility to establish regions for pear varieties is restricted, we have to create a particular micro-environment. Two groups are potential. The first one comprises sites where the annual precipitation is 700 mm, at least. There, apple and pear production would compete each other. In more dry habitats (less than 700 mm annual precipitation), micro-environments should be found and only drought-resistant, mainly summer-ripe cultivars should be chosen with, preferably, low tendency of sclereid formation. In that case, neither irrigation could help to produce adequate quality in varieties sensitive to low air humidity.
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Development of microsatellite markers for Rhodiola rosea
37-42.Views:217Rhodiola rosea L. is an important adaptogen medicinal plant. In this study two new microsatellite markers were developed. The assessment of the genetic diversity of R. rosea has recently started with molecular markers, but only a few species-specific microsatellite markers have been published so far. However the small number of markers allows only a limited insight into the genetic variability of the species therefore the aim of our work was to develop new microsatellite markers for R. rosea with a microsatellite enrichment library technique. Genomic DNA was cleaved with an endonuclease enzyme followed by adaptor ligation and PCR amplification. DNA fragments that contained microsatellites were first isolated using a biotin-streptavidin linkage based magnetic selection and then cloned into plasmids. Out of forty-three sequenced clones three contained microsatellites, in these cases primers were designed for the amplification of the microsatellite repeats. The newly developed primer pairs were tested on individuals from distant R. rosea populations and the variability of the amplified fragments was estimated by fragment-length analysis. The locus RhpB14a was found to be monomorphic while RhpB14b and RhpB13 were polymorphic. As a result of the present study, two novel variable microsatellite loci were identified in the genome of R. rosea.
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Effect of Erwinia amylovora infection on peroxidase enzyme activity in resistant apple cultivars
37-39.Views:155Two apple cultivars that display enhanced resistance to fire blight (causal agent: Erwinia amylovora) were selected. The aim of the present study was to characterize the peroxidase (POD) enzyme activity of `Szemes alma' (a historical cultivar) and MR-03, (a Hungarian multiresistant hybrid of 'Prima') and compare them to susceptible 'Jonathan M 40' and resistant 'Remo' controls. Peroxides enzyme activity during E. amylovora infections was investigated in artificially infected apple shoots. Increases in enzyme activities were observed in a `Jonathan M40' and in 'Remo', MR-03, `Szemes alma' cultivars. There was a consistent relationship between total enzyme activity and fire blight disease severity. High activity of the peroxidase was positively correlated with the degree of resistance to fire blight. A general hypothesis that POD activity is related to fire blight susceptibility/resistance is supported by our results.
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The role of meteorological variables of blossoming and ripening within the tendency of qualitative indexes of sour cherry
7-10.Views:244We analyzed the relationship to sour cherry quality parameters of average temperature, maximum temperature, minimum
temperature, night and day-time temperature, precipitation and climatic water balance variables. Three cultivars of sour cherry were included
in the selection: “Debreceni bôtermô”, “Kántorjánosi”, and “Újfehértói fürtös”.We conducted regression analyzes on the three varieties, but
present only those that proved to be the best fit.We couldn’t find any significant differences in the weather tolerance of the varieties. The data
base of sour cherry quality parameters covers the 1998 to 2008 intervals. The researched parameters were the following: dry matter content
(%), sugar content (%), C-vitamin (mg), total acid content (%). Maximum temperature, the difference of night and day-time temperature and
minimum temperature all have a significant correlation with the investigated quality parameters of sour cherry. Minimum temperature shows
a quadratic relationship with sugar content, while night and day-time temperature differences show the same with dry matter content. The
other weather parameters had a linear correlation with the quality parameters of sour cherry. The research results show that an increasing
amount of precipitation causes decreasing tendency in total acid content of sour cherry, so that increasing temperature has a positive influence
on total acid content. Big differences in night and day-time temperature also has positive effects on the dry matter and sugar content of sour
cherry while the amount of precipitation has a negative effect. High minimum temperatures cause decreasing sugar content respiratory energy
losses are significant if dawn temperatures do not drop considerably.A more favourable climatic water balance has a positive effect on vitamin
C content in cases of a large negative water balance, vitamin C content is generally low. -
General defense system in the plant kingdom
79-84.Views:136The goal of plant breeders is to improve the resistance of crops against virus, bacterium and fungus pathogens was easiest to achieve by selection for phenotypes displaying the hypersensitive reaction. The resistant plant of that type keeps its health by preventing or delaying the systemization of the pathogen by destruction of cells and tissues of variable size or amputation of the contaminated organs. The faster the reaction of the host plant is the more efficient and economical is the defense, since the extent of tissue destruction decreases proportionally with the speed of reaction.
During a breeding program for resistance carried out on several plant species, mainly vegetables over thirty years, also an alternative defense reaction has been experienced, which fundamentally differs from the hypersensitive reaction. In that reaction the cells and tissues of the host plant being exposed to the pathogen do not die, on the contrary they hinder systemization of the pathogen by tissue thickening. An additional significant difference is that on the contrary to hypersensitive reaction this reaction is less host- or pathogen-specific and works excellently even at high temperature (over 40 °C).
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Promising black locust (Robinia pseudoacacia L.) cultivars in Hungary
18-20.Views:344In Hungary, black locust (Robinia pseudoacacia L.) is one of the most important exotic stand-forming tree species, growing mostly under unfavourable ecological conditions. Considering the climate change effects its importance is increasing in many other countries. As a result of a selection programme, several black locust cultivars have been improved for setting up cultivar trials. In the paper four black locust cultivars were evaluated in Central Hungary under arid hydrological and brown forest soil conditions. Significant differences (P<5%) were found in height, DBH, mean tree volume and average stem form value (SFV). At the age of 35 the cultivar ‘R.p. Jászkiséri’ appeared to be the most promising one for yield production and ‘R.p. Zalai’ for SFV.
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Pomology of gene resources bearing resistance to Erwinia amylovora
29-33.Views:128The ecological farming and an increasing demand for healthy fruits free from chemical residuals necessitated the reevaluation of the existing cultivars and breeding suitable cultivars for the new requirements. We have gathered the old genotypes known in the Carpathian Basin, and we have selected the most suitable ones according to fruit quality and resistance to diseases in the last fifteen years. The main aim was the selection of resistant genotypes against fire blight and powdery mildew as gene sources for our breeding program. On the other hand, we consider the best quality traditional cultivars as extensive landscape elements (wayside tree rows or extensive squash producing plantations).
During the pathological evaluation 'Szemes alma', 'Pónyik', 'Sikulai', 'Tordai piros 'cavil' and 'Szabadkai szercsika' cultivars proved to be good resistance sources (due to their outstanding resistance to fire blight). The latter four cultivars are not susceptible even to fungal diseases, and their quality is also acceptable so those are commendable for ecological farming or for renewing the sparse orchards. According to fruit quality and low susceptibility to diseases 'Batul' and 'Vilmos renet' can also be offered for extensive organic production.
We have made original or renewed pomological descriptions of the selected cultivars, and we also publish their characters according to the terms of UPOV TG/14/8 based on to our evaluations.
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Hydroponic pepper growing on baked clay pellets
37-40.Views:200Nowadays one of the most important issues of greenhouse vegetable production in soilless media is the protection of the environment, in particular, the selection of the root medium to be applied. The objective of the trial was to test the applicability of baked (expanded) clay granules in hydroponic pepper growing with special respect to the growing pot (plastic tubes and buckets with bottom and lateral holes). From the result of the experiment it can be concluded that baked clay pellets, similarly to rockwool, are a suitable medium for providing root anchorage for pepper, however, it is necessary to examine some technological issues (e.g. fertilization, irrigation) prior to starting a large scale commercial cultivation. Relative to the three growing containers tested, it can be concluded that with the 4-8 mm crushed clay pebbles cultivation can be carried out successfully both in the white plastic tube and in the bucket, with the latter it is recommended to locate the drainage holes on the side of the growing container (at 6 cm from the bottom of the bucket).
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Development of in vitro propagation system for Atriplex halimus L.
123-129.Views:218Explants excised from adult shrubs were surface sterilized and cultured on Murashige and Skoog (MS) basal medium in the presence of plant growth regulators (PGRs) at different concentrations. A high multiplication rate of 7.2-fold was achieved every four weeks on MS medium supplemented with 4.44 μM BA, 0.49 μM IBA and 0.58 μM GA3. Rooting was achieved with 73% efficiency within 2-4 weeks on agar-gelled MS basal medium free of PGRs. Rooted plantlets were gradually acclimatized to field conditions over 5-6 weeks with 65% efficiency. For in vitro selection for salt tolerance, MS medium was supplemented with increasing concentrations of NaCl ranging between 25 and 1000 mM. This study has demonstrated that in vitro shoots could tolerate up to 600 mM NaCl with optimal growth at 200 mM, while higher concentrations of NaCl affected growth negatively. Growth and shoot number decreased with increasing NaCl concentration with all plantlets died at 1000 mM NaCl.
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Malus taxa and the progenies of Malus floribunda selected in Hungary, as gene sources of resistance breeding
25-28.Views:127One of our objectives in the apple breeding program of the Department of Fruit Science, beside creating new varieties, is to search for new gene sources. After evaluating the disease resistance of Malus taxa available in Hungary, we put the promising selected individuals through further examinations. Their habit and growth vigor was observed, as well as productivity and fruit characteristics were evaluated. Malus fusca seems to be a new, promising gene source in Hungary among the examined taxa. Beside its optimal canopy, growth vigor and fruit characteristics, it has the advantage to be in distant relationship to Malus x domestica, therefore its resistance is based on a different genetic basis. SBG 1 selection of Malus spectabilis can be recommended as a new gene source for resistance breeding. The range of gene sources can further be widened by selected clones of Malus floriblunda (BA I), Malus x zumi (BA) and Malus baccata (SBG 6). An additional value of Malus taxa chosen by us is that they show resistance not only to apple scab, but to powdery mildew as well, and according to our results, they have optimal habit, acceptable productivity and good fruit quality. Nine hybrids were selected from first Malus progenies which proved to be resistant to apple scab and powdery mildew. These are valuable not only as gene sources of breeding, but as pollenizers or ornamental trees, while their decorative fruits can be used in inside decorations and flower arrangements.
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Fire blight (Erwinia amylovora) resistance in apple varieties associated with molecular markers
53-57.Views:230The invasive bacterial disease fire blight, caused by Erwinia amylovora has the potential to destroy fruit tree orchards all over Europe. Effective plant protection methods are lacking in many countries, highlighting the increasing importance placed on identification of germplasm with heritable disease resistance. Recent l y. a promising QTL (quantitative trait locus) was identified on linkage group 7 in the apple cultivar 'Fiesta· which is derived from ·cox's Orange Pippin' . I n the present study, 144 Swedish and foreign apple cultivars were analysed with the SCAR markers AE I 0-375 and GE-8019. which flank-. this QTL. Twenty-nine of the analysed cultivars had both markers. 78 had either AE I 0-375 or GE-8019, and 37 cultivars did not carry an) of the two markers. Seventeen cultivars. 7 with both markers and I 0 not having either of the two markers, were then inocoluted with the bacterium in a 4uaran1i ne greenhouse test. Cultivars carrying both DNA markers were significantly less susceptible than cultivars lacking the markers, P<0.001. Cultivars that were most resistant had both markers and had 'Cox· in their pedigree. Unrelated cultivars with the markers may still lack the QTL.
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Effect of different cultivation methods on strawberry’s antioxidant value
7-10.Views:389The small fruit and strawberry production is economically important in Serbian agriculture. The fresh fruit is sold in domestic markets, but the frozen and processed is mainly sold in foreign markets. The growers find themselves in an ever-expanding selection of the varieties, but besides the nursery and breeder’s descriptions of new cultivars, there are only few exact results for the biological and economical ability of the varieties, which makes easier to choose the proper cultivar. The performance of the varieties in modern strawberry production is worth to investigate by adaptive cultivation methods and production technique. In the case of new strawberry varieties we know neither their needs in production nor their real capability, so they have to be compared with an older, standard control variety (‘Clery’). The breeder’s manuals give only references for the needs of the production technique, of the variety, so it is necessary to have facts, which are based on objective testing results. In this work, a new variety (‘Joly’) is being compared with the control variety. Comparative study of individual varieties are required covering the antioxidant content of the fruits (FRAP) and variability of these amounts in different years of breeding as well as the production techniques under similar circumstances.
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Six promising selections from the Hungarian apple breeding program for multiple resistance
23-28.Views:140An apple breeding program has been carried out at the Department of Fruit Science for more than a decade. Several apple selections have been released from the progenies of crosses in 1992 and 1993. Six candidates were submitted for national recognition out of the hybrids examined for more than a decade. The six selections are resistant against all the three most important apple diseases (apple scab, powdery mildew and fire blight). Scab resistance is controlled by the Vf gene originating from the species Malus floribunda 821 and transmitted by cultivar Prima. Heterozygote Vfvf genotype of the six cultivar candidates was proved by molecular genetic examinations of Dept. Genetics and Plant Breeding. Characteristics of these selections from 'Prima' progenies are shown on the base of our own observations.
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The importance of pathogen-free grapevine propagation material in regards to clonal selection and rootstock breeding in Germany
11-13.Views:211High performing propagation material is essential for a reliable and economical production of quality grapes. Apart from genetic aspects pathogen-freedom is of prime importance in propagation material. In particular virus diseases cause major yield and quality losses and reduced longevity. This is also reflected in the current EU legislation, which focuses on the most common and dangerous viruses: GFLV, ArMV, GLRaV-I and GLRaV-III.Apart from these, locally occurring pathogens, e.g. phytoplasms or agrobacterium, are important as well and should not be present in propagation material. There are several ways to develop pathogen-free clones. Starting with already pathogen-free material is certainly the easiest case, but might not be feasible in local varieties with small acreages and limited vine numbers. In these cases the elimination of pathogens is required, either by heat therapy, tissue culture or somatic embryo genesis.
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Work organization and economic analysis of the postharvest of an unique Hungarian product
11-14.Views:133We have viewed a business in the south of the Great Plain Region from an economic point of view in 2004-2005. The main activity there is pear growing and storage. Four varieties of different time of ripening and storing are grown there. We have measured all the relevant activities, worked out a local normative and prepared a detailed technology. The economic evaluation was based on this data. Activities, like disinfection, pre-storage disinfection and selection, in-storing and out-storing, classification after storage, packaging, as well as loading trucks, were monitored by variety. Storage loss was determined and widely varied according to varieties, length of storage and time of out-storing. Different varieties resulted in different quality classes after storage. Price depended on the quality classes. Economic evaluation was carried out when all the relevant costs and revenues were known. Fixed and variable costs of storage were determined, break-even point was calculate and the market position of the product was evaluated.
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The uses of wolfberry (Lycium barbarum L.) as a fruit in an international breadth of view
7-13.Views:281Lycium barbarum is known exclusively as an ornamental plant in Hungary, and is planted so, as popular belief deem it a toxic plant. The plant’s fruit receives great respect in countries abroad, thus, due to its favourable content values the Lycium has achieved the title of ‘biological gold mine’. The last couple of years has seen the import of Lycium shrubs and its corresponding products, dried goods or in the form of various processed products which have been marketed and sold at extreme prices (under name of Goji, Wolfberry, Lifeberry). Our goal was to examine the similarities of the content values of wolfberry found in Hungary and those cultivated abroad. Along with Miklós Józsa the domestic Lycium population was surveyed based on foreign examples, between the years 2009 and 2011. Those defined sweet and largefruited were selected for further investigations to be set into a clone repository. This clone repository– which contains 67 different clones from a number of regions of Hungary – was established in the nursery of Dr. Miklós Józsa, located in the city of Szombathely. The phenological and morphological characteristics and the fruit ripening and quality indicators of plants in the clone repository were investigated. Six ‘best’ clones – selected based on flavour, disease-resistance and vegetative characteristics (plant size, fruit set, yield and fruit size) were analysed based on their content values. The control plant was a cultivar imported by a delivery service, found also in growing. In addition to the results of the selection, the results of the content values of the six selected ‘best’ clones (total soluble sugar content, glucose-fructose ratio, carotene content, FRAP value) is documented in this paper. Further, based on the resulting information the possibilities of the fruit’s utilization are suggested. Significant differences were measured in the vegetative characteristics of the clones (plant size, fruit set, yield and fruit size) and of those of
its inner content values. The resulting properties are considerable in regards of the plant’s introduction into cultivation and also utilization. Based on growing characteristics, more favourable clones were selected than the foreign varieties already in growing. Those clones selected by us have had similar or better content values than foreign breeds. The investigation of these content values is still in progress. -
Grape variety comparison of different stress tolerance based on the quantitative measurement of carbohydrates
37-40.Views:167The analyses of various host-pathogen relationships have established the response reaction roles of carbohydrates — especially monosaccharides — measurable in the vegetal parts of the host. Published results also provide information concerning the way various pathogens utilize carbohydrates and concerning the carbohydrates pathogens prefer out of the "selection" provided by the host plant. The role of carbohydrates in the response reactions to abiotic stress has been studied on several plant species as well — currently, too, it is an often discussed area of research. The above-mentioned results form the basis of our intention to study the connection between susceptibility to grey mould and the quantity of measurable carbohydrates in the leaves of grape varieties of various stress tolerance levels.
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Sampling experience in a cherry plantation
21-28.Views:287Experiments in a cherry plantation were performed to find out that samples taken from various parts of the foliage of the tree what extent represent the whole tree. One tree from three different cherry varieties was selected. In the selection, we tried to form a good representation of the foliage of the orchard. 8 sampling locations were designated on each tree, in two different heights and four different directions according to the points of the compass.We measured the main sizes of the fruits in three orthogonal dimensions with a digital slide gauge with an accuracy of 0.01 mm; the mass of each cherry by an analytical balance, with an accuracy of 0.001 g; the mass of the stones with the same analytical balance, and then calculated the stone-pulp ratio. The measured and calculated data are used to characterize the sample in question.
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Estimation of plum and prune cultivars with morphogenetical traits
147-152.Views:194The author post few years organizes the plum breeding program. He uses up earlier elaborated morphogenetic and productive characteristics and traits, already on such basis their selected 21 cultivars in the pomological garden at Cegléd. The Hungarian plum assortment and offer very specific and tight, thus that they endeavor, that the selection before then flare. There are indicating already only the perspective cultivars in present paper on basis of gathered data one decade underneath. Paralleling control was three type of used (Besztercei szilva Bt. 2, Green gage and Stanley). 12-sort trait according to were compared the cultivars. The productivity-biological standpoints without, the open pollination and pollen germination, over and above that the ripening on date big differences appear. The near future the 'C. 1501' (Yellow Besztercei), 'Ontario' and `Mirabelle de Nancy' (yellow plums), the yes untimely `Precoce di Guigno' (red plum), the very well abundant 'C. 940' and Victoria (lilac plums), over and above that 'Beregi datolya', ‘Révfülöpi’ and `Szarvasi' (blue plums) cultivars setup suggest. The demonstrated cultivars out of further give for deputize value the Precoce di Giugno, as earliest ripening and the 'Beregi datolya' but the at the latest ripening.
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Inheritance of the characters related to flower formation, blooming and fertilisation in apple
63-65.Views:169On the base of observations performed during a period of 20 years the blooming characters-of apple varieties and their progenies the following statements are actual.
In blooming dynamics there was no difference between paternal and maternal effects. In the assignment to blooming time groups, the paternal effect prevailed whereas in the tendency of flower initiation on long shoots maternal parent was more decisive. Varieties as 'Golden Delicious'. 'Jonathan', 'Red Delicious', 'Rome Beauty' and 'Staymared' and their respective, naturally raised mutants did not differ in blooming characters.
The possibility of predicting the relation to blooming time groups of early (July, August) ripening individuals is low, whereas late (September. October) ripening ones have a good chance to be medium late in blooming time.
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Comparison of light yellow fleshed pepper varieties grown on rockwool under unheated forcing conditions
108-110.Views:108Experiments are going on all over the world assisting the joint effort of researchers and practicing specialists to identify the methods which can help either in the reduction of production costs or in the increase of yields.
The task of the growers is to make profitable use of the forcing facilities and to satisfy market demands at an acceptable price by means of improving production technology and applying new scientific, technological and technical information.
For the last few years, rockwool based forcing has been gaining in importance. The subject of our scientific work was the analysis of an important question of this technological variant, the selection of the variety. Besides, we also tried to identify the most suitable pruning technology for the varieties studied.
In Hungary, the highest demand is commonly known to be for the light yellow fleshed varieties which are suitable for stuffed dishes. In the future, due to their special quality and appearance, as well as to the Hungaricum character, they could become important export goods on the European Union market. It was within this variety type that comparison between varieties already common in production (HO F1, HRF F1, Danubia F1) was carried out, trying to get an answer to the question which of the three varieties could be produced with the greatest success. Considering the quantitative and qualitative indicators, it was H6 F1 that proved the best out of the three varieties tested under unheated forcing on rockwool. It excelled the other two varieties both in quality and in average fruit weight, preserving this advantage until the end of the growing period.
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Promising white poplar (Populus alba L.) clones in sandy ridges between the rivers Danube and Tisza in Hungary
113-116.Views:189White poplar is a native stand-forming tree species in Hungary, covering 3.1 per cent of the forested area. More than 70 per cent of the white poplar stands can be found on calcareous sandy sites in the Danube—Tisza region, so they play a significant role in the poplar management of this part of the country. The most important task ahead of Hungarian poplar growers is to improve the quality of poplar stands and plantations based on selecting new clones and cultivars. The growth and yield of four promising white poplar clones was evaluated on a marginal site in central Hungary. The clones `1-1 425-4' (Populus alba x Populus alba), and 11 758' (Populus alba Mosonmagyaróvár 124) seem to be suitable for wood production, while the 427-3' (Populus alba x Populus alba cv. Bolleana) and 422-9' (Populus alba x Populus grandidentata) clones (with decorative stem form) could be better used for tree lines and ornamental plantations.
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Morphological and phenological properties of sour cherry varieties grown in Hungary and their inter-incompatibility relations
114-117.Views:175Regular observations and experiments were performed during a 14 year period on 6 sour cherry varieties. The morphological traits of leaves and fruits were compared, and the phenology of blooming as well as of ripening dates served to start an estimation of the possibilities of mutual pollination and the planning of harvest operations. Experiments involved obligate autogamy, artificially controlled allogamy and open pollination in order to reveal self-fertility, self-sterility or inter-incompatibility relations.
The varietal characters represent, each, different values in the distinction of the items, because of their intra-varietal variability. From that point of view, the most reliable are the data of blooming and ripening time, fruit size and the fertility relations.
Inter-incompatibility was observed between the group of self-fertile, "Pándy type" varieties (`Újfehértói fürtös’, ‘Debreceni bőtermő’, ‘Kántorjánosi’) on one side and the selection of Pándy 7', a self-sterile variety on the other side. Unilateral incompatibility has been detected within the former group of new, self-fertile varieties, the combinations: (`Újfehértói fürtös’ x ‘Debreceni bőtermő’ as well as `Újfehértói fürtös’ x Kántorjánosi’.
Our results prove the close kinship between those three new varieties and the original Pándy variety on the base of being highly similar in their morphology and also of the fact of their inter-incompatibility, though unilateral.
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Study of different factors of grapevine regeneration systems and genetic transformation
33-36.Views:214The most limitating factor for successful transformation is the absence of high-yielding regeneration protocols. However, the anther-derived embryogenic culture is an optimal technique for genetic transformation and it has been widely applied in many important cultivars, but the necessity of further development of regeneration systems has been proved. We attempted to produce somatic embryos on a wide range of genotypes from various tissues; leaves, petioles, stem segments. We started the examination of grapevine regeneration via organogenesis, succeeded in inducing shoot from the meristematic tissue of the base of bud by testing induction medium contained different concentrations of two types of hormones. To optimize the conditions of the Agrobacterium-mediated transformation, we studied the effectiveness of different Agrobacterium-treatments, the use of antioxidants and the sufficient quantity of kanamycin for selection of transformed cells.