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• DNA-based determination of suitable pollinating cultivars for the pear cultivar 'Carola' (Pyrus communis)

  15-19.

  H. Nybom

  J. Sehic

  H. Mattisson

  Views:

  131

  Pollen-limited fruit set has long been suspected in some relatively low-yielding orchards with the Swedish pear cultivar 'Carola'. Fruit was therefore harvested on 23 'Carola' trees in a commercial pear orchard. The seeds were germinated and five seedlings from each tree were sampled to determine which of the surrounding cultivars had been the most successful pollinators. Leaves of 'Carola', the 7 putative pollinating cultivars and the 115 seedlings were analysed with 6 RAPD primers. By comparison of the band patterns, paternity could be ascertained for 74 seedlings. The by far most successful pollinator was 'Clara Frijs' which had sired approx. half of the seedlings, followed by 'Herzogin Elsa', `Skanskt Sockerpiiron', 'Alexandre Lucas', 'Coloree de Juillet' and 'Doyenne du Cornice'. The latter is the maternal parent of 'Carola', and these two cultivars must therefore share one S-allele and hence can only be semi-compatible. In addition, 6% of the seedlings were in all likelihood derived from selling_ since they showed no bands that did not occur also in 'Carola'. Maximum distance between 'Carola' trees and suitable pollinators should not exceed 15-20 tn. Longer distances may produce a serious dearth of compatible pol­len as evidenced by the large percentage of seedlings derived either from selling. (25%) or from long-distance (> 40 m) pollen transfer (25%) when 'Carola' trees were surrounded by non-preferred pollinators.

• Application of DNA markers for detection of scab resistant apple cultivars and selections

  59-63.

  H. Mattisson

  H. Nybom

  Views:

  103

  A DNA marker-based study was undertaken to identify the occurrence of major scab resistance genes in some apple cultivars and selections of importance for apple breeding. Unfortunately none of the RAPD-based markers previously reported to detect the V_a, V_b, V_r and V_x genes produced unambiguous results. By contrast, the CAPS marker MI8 produced the expected three bands in all cultivars and selections already known or suspected to have the V_f gene, as well as in the Russian cultivar 'Antonovka Polotora Funtovaja' suspected to have V_a resistance which however may be allelic to V_f. V_f-carrying selections and newly named cultivars 'Frida' and 'Fredrik' are grown successfully in Sweden without fungicides, suggesting that the V_r resistance breaking scab races 6 and 7 have not yet become a problem. The SCAR marker B12 detected the V_m gene in 'Prairifire', 'Rouville', clones 'OR45T132' and 'OR48T70', and selection '16-36-193'. The SSR locus 0102b10 detected one band at 118 by in 'Reka'. This is presumed to be identical to the V_r gene marker previously reported.