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Effect of Erwinia amylovora infection on peroxidase enzyme activity in resistant apple cultivars
37-39.Views:140Two apple cultivars that display enhanced resistance to fire blight (causal agent: Erwinia amylovora) were selected. The aim of the present study was to characterize the peroxidase (POD) enzyme activity of `Szemes alma' (a historical cultivar) and MR-03, (a Hungarian multiresistant hybrid of 'Prima') and compare them to susceptible 'Jonathan M 40' and resistant 'Remo' controls. Peroxides enzyme activity during E. amylovora infections was investigated in artificially infected apple shoots. Increases in enzyme activities were observed in a `Jonathan M40' and in 'Remo', MR-03, `Szemes alma' cultivars. There was a consistent relationship between total enzyme activity and fire blight disease severity. High activity of the peroxidase was positively correlated with the degree of resistance to fire blight. A general hypothesis that POD activity is related to fire blight susceptibility/resistance is supported by our results.
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Development of microsatellite markers for Rhodiola rosea
37-42.Views:201Rhodiola rosea L. is an important adaptogen medicinal plant. In this study two new microsatellite markers were developed. The assessment of the genetic diversity of R. rosea has recently started with molecular markers, but only a few species-specific microsatellite markers have been published so far. However the small number of markers allows only a limited insight into the genetic variability of the species therefore the aim of our work was to develop new microsatellite markers for R. rosea with a microsatellite enrichment library technique. Genomic DNA was cleaved with an endonuclease enzyme followed by adaptor ligation and PCR amplification. DNA fragments that contained microsatellites were first isolated using a biotin-streptavidin linkage based magnetic selection and then cloned into plasmids. Out of forty-three sequenced clones three contained microsatellites, in these cases primers were designed for the amplification of the microsatellite repeats. The newly developed primer pairs were tested on individuals from distant R. rosea populations and the variability of the amplified fragments was estimated by fragment-length analysis. The locus RhpB14a was found to be monomorphic while RhpB14b and RhpB13 were polymorphic. As a result of the present study, two novel variable microsatellite loci were identified in the genome of R. rosea.
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General defense system in the plant kingdom
79-84.Views:132The goal of plant breeders is to improve the resistance of crops against virus, bacterium and fungus pathogens was easiest to achieve by selection for phenotypes displaying the hypersensitive reaction. The resistant plant of that type keeps its health by preventing or delaying the systemization of the pathogen by destruction of cells and tissues of variable size or amputation of the contaminated organs. The faster the reaction of the host plant is the more efficient and economical is the defense, since the extent of tissue destruction decreases proportionally with the speed of reaction.
During a breeding program for resistance carried out on several plant species, mainly vegetables over thirty years, also an alternative defense reaction has been experienced, which fundamentally differs from the hypersensitive reaction. In that reaction the cells and tissues of the host plant being exposed to the pathogen do not die, on the contrary they hinder systemization of the pathogen by tissue thickening. An additional significant difference is that on the contrary to hypersensitive reaction this reaction is less host- or pathogen-specific and works excellently even at high temperature (over 40 °C).
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The role of meteorological variables of blossoming and ripening within the tendency of qualitative indexes of sour cherry
7-10.Views:215We analyzed the relationship to sour cherry quality parameters of average temperature, maximum temperature, minimum
temperature, night and day-time temperature, precipitation and climatic water balance variables. Three cultivars of sour cherry were included
in the selection: “Debreceni bôtermô”, “Kántorjánosi”, and “Újfehértói fürtös”.We conducted regression analyzes on the three varieties, but
present only those that proved to be the best fit.We couldn’t find any significant differences in the weather tolerance of the varieties. The data
base of sour cherry quality parameters covers the 1998 to 2008 intervals. The researched parameters were the following: dry matter content
(%), sugar content (%), C-vitamin (mg), total acid content (%). Maximum temperature, the difference of night and day-time temperature and
minimum temperature all have a significant correlation with the investigated quality parameters of sour cherry. Minimum temperature shows
a quadratic relationship with sugar content, while night and day-time temperature differences show the same with dry matter content. The
other weather parameters had a linear correlation with the quality parameters of sour cherry. The research results show that an increasing
amount of precipitation causes decreasing tendency in total acid content of sour cherry, so that increasing temperature has a positive influence
on total acid content. Big differences in night and day-time temperature also has positive effects on the dry matter and sugar content of sour
cherry while the amount of precipitation has a negative effect. High minimum temperatures cause decreasing sugar content respiratory energy
losses are significant if dawn temperatures do not drop considerably.A more favourable climatic water balance has a positive effect on vitamin
C content in cases of a large negative water balance, vitamin C content is generally low. -
Promising black locust (Robinia pseudoacacia L.) cultivars in Hungary
18-20.Views:332In Hungary, black locust (Robinia pseudoacacia L.) is one of the most important exotic stand-forming tree species, growing mostly under unfavourable ecological conditions. Considering the climate change effects its importance is increasing in many other countries. As a result of a selection programme, several black locust cultivars have been improved for setting up cultivar trials. In the paper four black locust cultivars were evaluated in Central Hungary under arid hydrological and brown forest soil conditions. Significant differences (P<5%) were found in height, DBH, mean tree volume and average stem form value (SFV). At the age of 35 the cultivar ‘R.p. Jászkiséri’ appeared to be the most promising one for yield production and ‘R.p. Zalai’ for SFV.
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Pomology of gene resources bearing resistance to Erwinia amylovora
29-33.Views:120The ecological farming and an increasing demand for healthy fruits free from chemical residuals necessitated the reevaluation of the existing cultivars and breeding suitable cultivars for the new requirements. We have gathered the old genotypes known in the Carpathian Basin, and we have selected the most suitable ones according to fruit quality and resistance to diseases in the last fifteen years. The main aim was the selection of resistant genotypes against fire blight and powdery mildew as gene sources for our breeding program. On the other hand, we consider the best quality traditional cultivars as extensive landscape elements (wayside tree rows or extensive squash producing plantations).
During the pathological evaluation 'Szemes alma', 'Pónyik', 'Sikulai', 'Tordai piros 'cavil' and 'Szabadkai szercsika' cultivars proved to be good resistance sources (due to their outstanding resistance to fire blight). The latter four cultivars are not susceptible even to fungal diseases, and their quality is also acceptable so those are commendable for ecological farming or for renewing the sparse orchards. According to fruit quality and low susceptibility to diseases 'Batul' and 'Vilmos renet' can also be offered for extensive organic production.
We have made original or renewed pomological descriptions of the selected cultivars, and we also publish their characters according to the terms of UPOV TG/14/8 based on to our evaluations.
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Hydroponic pepper growing on baked clay pellets
37-40.Views:187Nowadays one of the most important issues of greenhouse vegetable production in soilless media is the protection of the environment, in particular, the selection of the root medium to be applied. The objective of the trial was to test the applicability of baked (expanded) clay granules in hydroponic pepper growing with special respect to the growing pot (plastic tubes and buckets with bottom and lateral holes). From the result of the experiment it can be concluded that baked clay pellets, similarly to rockwool, are a suitable medium for providing root anchorage for pepper, however, it is necessary to examine some technological issues (e.g. fertilization, irrigation) prior to starting a large scale commercial cultivation. Relative to the three growing containers tested, it can be concluded that with the 4-8 mm crushed clay pebbles cultivation can be carried out successfully both in the white plastic tube and in the bucket, with the latter it is recommended to locate the drainage holes on the side of the growing container (at 6 cm from the bottom of the bucket).
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Malus taxa and the progenies of Malus floribunda selected in Hungary, as gene sources of resistance breeding
25-28.Views:119One of our objectives in the apple breeding program of the Department of Fruit Science, beside creating new varieties, is to search for new gene sources. After evaluating the disease resistance of Malus taxa available in Hungary, we put the promising selected individuals through further examinations. Their habit and growth vigor was observed, as well as productivity and fruit characteristics were evaluated. Malus fusca seems to be a new, promising gene source in Hungary among the examined taxa. Beside its optimal canopy, growth vigor and fruit characteristics, it has the advantage to be in distant relationship to Malus x domestica, therefore its resistance is based on a different genetic basis. SBG 1 selection of Malus spectabilis can be recommended as a new gene source for resistance breeding. The range of gene sources can further be widened by selected clones of Malus floriblunda (BA I), Malus x zumi (BA) and Malus baccata (SBG 6). An additional value of Malus taxa chosen by us is that they show resistance not only to apple scab, but to powdery mildew as well, and according to our results, they have optimal habit, acceptable productivity and good fruit quality. Nine hybrids were selected from first Malus progenies which proved to be resistant to apple scab and powdery mildew. These are valuable not only as gene sources of breeding, but as pollenizers or ornamental trees, while their decorative fruits can be used in inside decorations and flower arrangements.
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Development of in vitro propagation system for Atriplex halimus L.
123-129.Views:208Explants excised from adult shrubs were surface sterilized and cultured on Murashige and Skoog (MS) basal medium in the presence of plant growth regulators (PGRs) at different concentrations. A high multiplication rate of 7.2-fold was achieved every four weeks on MS medium supplemented with 4.44 μM BA, 0.49 μM IBA and 0.58 μM GA3. Rooting was achieved with 73% efficiency within 2-4 weeks on agar-gelled MS basal medium free of PGRs. Rooted plantlets were gradually acclimatized to field conditions over 5-6 weeks with 65% efficiency. For in vitro selection for salt tolerance, MS medium was supplemented with increasing concentrations of NaCl ranging between 25 and 1000 mM. This study has demonstrated that in vitro shoots could tolerate up to 600 mM NaCl with optimal growth at 200 mM, while higher concentrations of NaCl affected growth negatively. Growth and shoot number decreased with increasing NaCl concentration with all plantlets died at 1000 mM NaCl.
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Fire blight (Erwinia amylovora) resistance in apple varieties associated with molecular markers
53-57.Views:223The invasive bacterial disease fire blight, caused by Erwinia amylovora has the potential to destroy fruit tree orchards all over Europe. Effective plant protection methods are lacking in many countries, highlighting the increasing importance placed on identification of germplasm with heritable disease resistance. Recent l y. a promising QTL (quantitative trait locus) was identified on linkage group 7 in the apple cultivar 'Fiesta· which is derived from ·cox's Orange Pippin' . I n the present study, 144 Swedish and foreign apple cultivars were analysed with the SCAR markers AE I 0-375 and GE-8019. which flank-. this QTL. Twenty-nine of the analysed cultivars had both markers. 78 had either AE I 0-375 or GE-8019, and 37 cultivars did not carry an) of the two markers. Seventeen cultivars. 7 with both markers and I 0 not having either of the two markers, were then inocoluted with the bacterium in a 4uaran1i ne greenhouse test. Cultivars carrying both DNA markers were significantly less susceptible than cultivars lacking the markers, P<0.001. Cultivars that were most resistant had both markers and had 'Cox· in their pedigree. Unrelated cultivars with the markers may still lack the QTL.
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Effect of different cultivation methods on strawberry’s antioxidant value
7-10.Views:377The small fruit and strawberry production is economically important in Serbian agriculture. The fresh fruit is sold in domestic markets, but the frozen and processed is mainly sold in foreign markets. The growers find themselves in an ever-expanding selection of the varieties, but besides the nursery and breeder’s descriptions of new cultivars, there are only few exact results for the biological and economical ability of the varieties, which makes easier to choose the proper cultivar. The performance of the varieties in modern strawberry production is worth to investigate by adaptive cultivation methods and production technique. In the case of new strawberry varieties we know neither their needs in production nor their real capability, so they have to be compared with an older, standard control variety (‘Clery’). The breeder’s manuals give only references for the needs of the production technique, of the variety, so it is necessary to have facts, which are based on objective testing results. In this work, a new variety (‘Joly’) is being compared with the control variety. Comparative study of individual varieties are required covering the antioxidant content of the fruits (FRAP) and variability of these amounts in different years of breeding as well as the production techniques under similar circumstances.
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Six promising selections from the Hungarian apple breeding program for multiple resistance
23-28.Views:129An apple breeding program has been carried out at the Department of Fruit Science for more than a decade. Several apple selections have been released from the progenies of crosses in 1992 and 1993. Six candidates were submitted for national recognition out of the hybrids examined for more than a decade. The six selections are resistant against all the three most important apple diseases (apple scab, powdery mildew and fire blight). Scab resistance is controlled by the Vf gene originating from the species Malus floribunda 821 and transmitted by cultivar Prima. Heterozygote Vfvf genotype of the six cultivar candidates was proved by molecular genetic examinations of Dept. Genetics and Plant Breeding. Characteristics of these selections from 'Prima' progenies are shown on the base of our own observations.
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The importance of pathogen-free grapevine propagation material in regards to clonal selection and rootstock breeding in Germany
11-13.Views:203High performing propagation material is essential for a reliable and economical production of quality grapes. Apart from genetic aspects pathogen-freedom is of prime importance in propagation material. In particular virus diseases cause major yield and quality losses and reduced longevity. This is also reflected in the current EU legislation, which focuses on the most common and dangerous viruses: GFLV, ArMV, GLRaV-I and GLRaV-III.Apart from these, locally occurring pathogens, e.g. phytoplasms or agrobacterium, are important as well and should not be present in propagation material. There are several ways to develop pathogen-free clones. Starting with already pathogen-free material is certainly the easiest case, but might not be feasible in local varieties with small acreages and limited vine numbers. In these cases the elimination of pathogens is required, either by heat therapy, tissue culture or somatic embryo genesis.
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Work organization and economic analysis of the postharvest of an unique Hungarian product
11-14.Views:124We have viewed a business in the south of the Great Plain Region from an economic point of view in 2004-2005. The main activity there is pear growing and storage. Four varieties of different time of ripening and storing are grown there. We have measured all the relevant activities, worked out a local normative and prepared a detailed technology. The economic evaluation was based on this data. Activities, like disinfection, pre-storage disinfection and selection, in-storing and out-storing, classification after storage, packaging, as well as loading trucks, were monitored by variety. Storage loss was determined and widely varied according to varieties, length of storage and time of out-storing. Different varieties resulted in different quality classes after storage. Price depended on the quality classes. Economic evaluation was carried out when all the relevant costs and revenues were known. Fixed and variable costs of storage were determined, break-even point was calculate and the market position of the product was evaluated.
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The uses of wolfberry (Lycium barbarum L.) as a fruit in an international breadth of view
7-13.Views:260Lycium barbarum is known exclusively as an ornamental plant in Hungary, and is planted so, as popular belief deem it a toxic plant. The plant’s fruit receives great respect in countries abroad, thus, due to its favourable content values the Lycium has achieved the title of ‘biological gold mine’. The last couple of years has seen the import of Lycium shrubs and its corresponding products, dried goods or in the form of various processed products which have been marketed and sold at extreme prices (under name of Goji, Wolfberry, Lifeberry). Our goal was to examine the similarities of the content values of wolfberry found in Hungary and those cultivated abroad. Along with Miklós Józsa the domestic Lycium population was surveyed based on foreign examples, between the years 2009 and 2011. Those defined sweet and largefruited were selected for further investigations to be set into a clone repository. This clone repository– which contains 67 different clones from a number of regions of Hungary – was established in the nursery of Dr. Miklós Józsa, located in the city of Szombathely. The phenological and morphological characteristics and the fruit ripening and quality indicators of plants in the clone repository were investigated. Six ‘best’ clones – selected based on flavour, disease-resistance and vegetative characteristics (plant size, fruit set, yield and fruit size) were analysed based on their content values. The control plant was a cultivar imported by a delivery service, found also in growing. In addition to the results of the selection, the results of the content values of the six selected ‘best’ clones (total soluble sugar content, glucose-fructose ratio, carotene content, FRAP value) is documented in this paper. Further, based on the resulting information the possibilities of the fruit’s utilization are suggested. Significant differences were measured in the vegetative characteristics of the clones (plant size, fruit set, yield and fruit size) and of those of
its inner content values. The resulting properties are considerable in regards of the plant’s introduction into cultivation and also utilization. Based on growing characteristics, more favourable clones were selected than the foreign varieties already in growing. Those clones selected by us have had similar or better content values than foreign breeds. The investigation of these content values is still in progress. -
Grape variety comparison of different stress tolerance based on the quantitative measurement of carbohydrates
37-40.Views:158The analyses of various host-pathogen relationships have established the response reaction roles of carbohydrates — especially monosaccharides — measurable in the vegetal parts of the host. Published results also provide information concerning the way various pathogens utilize carbohydrates and concerning the carbohydrates pathogens prefer out of the "selection" provided by the host plant. The role of carbohydrates in the response reactions to abiotic stress has been studied on several plant species as well — currently, too, it is an often discussed area of research. The above-mentioned results form the basis of our intention to study the connection between susceptibility to grey mould and the quantity of measurable carbohydrates in the leaves of grape varieties of various stress tolerance levels.