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The estimation of pollen viability of snowdrop (Galanthus nivalis L.)
141-144.Views:236In the present experiment pollen viability of snowdrop were tested using four vital dyes (TTC, Baker' procedure, MTT and FDA) to determine their potential to differentiate fresh living pollen from pollen heated for 12 hours at 80 °C (killed pollen). We found that two of the four dyes previously employed to determine viability also stained killed pollen in the case of snowdrop, while the FDA and MIT did not. We suggest that the two latter are probably the best method to test snowdrop pollen viability, since they do not normally stain either killed or aborted pollen.
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Nutritional quality, fruit shape and relationships among exotic and local Capsicum pepper genotypes in Uganda
33-39.Views:528Twenty-one hot pepper genotypes comprising of local (15) and exotic (6) types (C. annuum, C. frutescens and C. chinense) were characterized for selected fruit traits after propagation in a glasshouse at the Makerere University Agricultural Research Institute Kabanyolo in Central Uganda using a completely randomized design with three replicates. Ripe fruits were harvested and analyzed; traits evaluated were all significantly different at P<0.05 with variations in quality attributes. The genotype OHA-B305-10 had the highest ascorbic acid content (128.86 mg/100 g) and is recommended for improvement of both local and exotic genotypes targeting the fresh market. Genotypes CAP0408-12 and UG2 WE0511-22, with highest total soluble solids (16.17 ºBrix) and dry matter content (28.59%), respectively should be used in improvements for industrial use or processing to products such as chilli powder or flakes. BRS-M205-04 with highest titratable acidity (1.04%) can be used in enhancing shelf life of genotypes with low titratable acids as well as for the fresh market. In spite of the intraspecific relationships among genotypes, significant differences were observed in their quantitative traits. These genotypes will, therefore, be useful in improving the quality of hot pepper fruit in Uganda.
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Bioactive phenols in leaves of Forsythia species
57-59.Views:403Nowadays a number of lignans (arctigenin, matairesinol, pinoresinol and phillygenin) have come to the fore in research due to their various biological activities. In this paper the accumulation of these constituents in leaf extracts of Forsythia plants (F. intermedia, F. ovata 'Robusta’ and `Tetragold', F. suspensa, F. viridissima) was quantified using a new isolation method, supercritical CO2 fluid extraction. The total phenolic and flavonoid contents, the antioxidant capacity and the aglycone lignan profile were determined in leaf extracts of Forsythia species. Within the phenols, the flavonoids were only present in small quantities, but the amount of aglycone lignans was extremely high. F. ovata `Robusta' had the highest total lignan content (103.8 mg/g) of all the Forsythia species. The main lignan in this species is arctigenin, which normally makes up about 60% of the total lignan content, but in the case of F. ovata `Robusta' this value was 96.1%. Since this arctigenin content is outstanding compared to that of other Forsythia species, it could be promising to develop a fermentation technology for the production of this natural compound.
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Allyl isothiocyanate baited traps to monitor cabbage flea beetles (Phyllotretra spp., Coleoptera: Chrysomelidae)
95-99.Views:406A new trapping concept has been proposed based on a volatile compound, allyl isothyocianate, known to be attractant to some of these insects for a long time.
(l) The first question was whether this compound is effectively attractive to all flea beetle species attacking cabbage under our conditions? Field experiments were made at different localities with non-sticky baited traps early and late spring. Eleven Phyllotreta species attacking cabbages were captured at baited traps most of them were first observed at this bait. So the bait has proved to be sufficient for use for trapping purposes effectively.
- Based on these findings a second question arose whether the captured samples reflected the specific composition of natural flea beetle populations at trapping localities? To reply the question field samples were taken at four different kinds of cabbage crops and at a fallow ground in the close vicinity by a manual sampler device suitable to detect the local composition of flea beetles and trapping was made parallel with baited and unbaited traps from early spring to early autumn. No significant differences were found between the specific structures of Phyllotreta assemblages sampled with the different methods applied. This means baited traps reflected the specific composition of local Phyllotreta populations fairly well.
- Thirdly, the most effective trap design was searched for. Some sticky and non-sticky trap designs which had been developed to capture other insects were compared. The tested sticky and funnel trap designs baited with allyl isothiocyanate captured large numbers of flea beetles attacking cabbages. Results showed that non-sticky funnel traps were more effective than sticky delta traps. Accordingly, non-sticky funnel trap designs can advantageously be used and could possibly be recommended in plant protection practice to monitor flea beetles attacking cabbages as their catching capacity is considerably greater than that of the delta type and additionally captured beetles are much cleaner, more intact and consequently their identification is much easier.
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The effect of rootstock on the nectar production of apple cultivar `Idared'
15-25.Views:236Approaches based on the daily rhythm of apple flowers provide a new stage in nectar research, where the synchronous functioning of sexual organs is studied. In the flower biological studies the insect attraction of flowers was also studied. The two most important factors of insect attraction are the pollen- and nectar production of the flowers. From 1993 to 1998 we studied the food, that flower had to offer for the pollinating insects at different times of the day.
Studies were carried out on 'Idared', one of the hybrids of 'Jonathan' apple cultivar. The fruit of 'Idared' is bigger than the fruit of `Jonathan'. It is bright red, transportable, has a bigger productivity and is not subject to Jonathan-spots. Concerning its inner characteristics, it is juicy, the flesh consistency is better than that of 'Jonathan', but its acid/sugar ratio is worse (Sansavini et al. 1981).
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Floral attractivity of pear cultivar 'Cinderi'
102-109.Views:407The regularities of primary attractivity have been studied at the pear cultivar `Cinderi' for two years. Nectar quickly evaporates from the totally open nectary surface of pear flowers exposed to environmental effects, and the rhythmicity of nectar secretion can be determined with difficulty. Flowers do not function according to a unified endogenous rhythm, the whole tree becomes continuously attractive for insects, since it attracts insects on more occasions during the day with some of its flowers. During the warm afternoon hours there is usually no measurable nectar production. Pollen shedding is most intensive in the afternoon hours. Pear flowers produce little and diluted nectar, which often does not come up to apicultural expectations. The age of the flower does not significantly affect the quantity and refraction of nectar. The flowers of pear cv. ‘Cinderi' are delayed homogamous.
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Agrobacterium transformation of Rhodiola sp.: current status and limitations
37-42.Views:329The study of secondary metabolites has led to the discovery of new drugs for treating human diseases. However, consistent plant supply can be challenging, leading to the use of plant tissue culture techniques such as hairy root culture. Hairy roots have stable genetics, lateral branching, and can produce secondary metabolites, including alkaloids, flavonoids, and terpenoids. Research on hairy roots as a subject began in the late 19th century, and for the last four decades, hairy roots have been utilized for producing secondary metabolites and recombinant proteins. This article focuses on Rhodiola species - genus of perennial plants that belongs to the family Crassulaceae - and its potential as a source of secondary metabolites using hairy root culture techniques. Rhodiola sp. is widely distributed throughout the Arctic regions of the Northern Hemisphere, with several species having significant medicinal properties. The article discusses the possible use of hairy root cultures for the production of Rhodiola secondary metabolites, including salidroside and rosavins, which have demonstrated significant pharmacological activity in various studies. The use of elicitation and genetic engineering techniques to boost secondary metabolite production in Rhodiola hairy roots is also explored. Overall, the article highlights the potential of Rhodiola hairy root cultures as a valuable source of secondary metabolites with medicinal properties. However, despite some studies Rhodiola hairy root induction and culturing still remains highly unexplored.
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Precision geoinformatical system of the pear gene-collection orchard
43-50.Views:886The principle task of the sustainable development is the preservation of the genetic variety, which is similar challenge in the horticulture regarding the sublimation of fruit species. The breeders of the traditional fruit strains give stock to the sustenance diversity of the agro-environment on the species and landscape level. In 2009, hyperspectral images have been taken by AISA Dual sensors from the pear gene pool in Újfehértó, Hungary. The hyperspectral data cube (in the wavelength range of 400-2500 nm, with 1.5 m ground resolution) ensured possibility to make the spectral library of pear species. In the course of the simultaneously field work the spatial position and individual extent of all pear trees was defined to set up a detailed GIS data base. The water stress sensitivity of single species and the descriptive spectral curves were determined with common evaluation of the spectral and spatial data. Based on the unique methodology processing and the hyperspectral data base suitable strains can be chosen for agro-environment and let take adaptive stocks regarding climate change into the genetic grafting work. Furthermore we could determine and map the sparsely species in the region with the help of the hyperspectral data.
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Organic versus integrated apple growing: II. differences in fruit quality parameters
61-63.Views:883The aim our study was to establish whether significant differences in nutrients uptake and quality of fruit exist
between organic and integrated grown apples. The study was performed at the orchard Fruit Research Station, University of Debrecen, at Debrecen-Pallag during 2002–2004. Diameter (mm), weight (g), firmness (N/cm2), dry matter (%) and Vitamin C in fruit were determined. Diameter data showed that the size of apples strongly depended on cultivars and organic apples were larger than integrated ones. Moreover, the effect of year and species strongly affected the apple diameter, size and weight. Measured data of firmness were good agreement in data of diameter and weight. Larger fruits have lower values of firmness due to the structure of fruit flesh. Dry matter content of apples varied between 14.66 and 18.07 in integrated, and 12.87 and 17.29 in organic apples according to cultivars. Values were affected by years and cultivars. From results it was evident that the dry matter content of apples affected by production system. It was lower in organic samples than integrated ones.Vitamin C content of apples was stronger affected by species and years than production system. -
Relationship between several meteorological factors and phenological features of apple cultivars
13-19.Views:1011The trees observed are grown at Ofeherto, Eastern Hungary in the plantation of an assortment (gene bank) with 586 apple cultivars. Each of the cultivars were observed as for their dates of subsequent phenophases, the beginning of bloom, main bloom and the end of bloom over a period between 1984 and 2001. during this period the meteorological data-base keeps the following variables: daily means of temperature (°C), daily maximum temperature (°C), daily minimum temperature (°C), daily precipitation sums (mm), daily sums of sunny hours, daily means of the differences between the day-time and night-time temperatures (°C), average differences between temperatures of successive daily means (°C). Between the 90th and 147th day of the year over the 18 years of observation. The early blooming cultivars start blooming at 10-21April. The cultivars of intermediate bloom start at the interval 20 April to 3 May, whereas the late blooming group start at 2-10 May. Among the meteorological variables of the former autumnal and hibernal periods, the hibernal maxima were the most active factor influencing the start of bloom in the subsequent spring.
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Review of the self-incompatibility in apple (Malus x domestica Borkh., syn.: Malus pumila Mill.)
31-36.Views:882Apple (Malus x domestica Borkh.) is one of the most important fruit crops showing ribonuclease-mediated self-incompatibility, and no self-compatible apple cultivars are known. Twenty-nine S-alleles were identified in apple and many more incompatibility groups are present compared to sweet cherry. Results from a Belgian, English and a Japanese research group are combined and the S-genotypes of the most important world cultivars are collected. Two different allele labelling system are reconciled and detection methods used in case of the specific alleles are shown. Effects of the resistance breeding programmes are discussed; and scientific efforts involving transgenic technology to create self-compatible genotypes are shown. This review covers the most interesting issues regarding self-incompatibility in apple.
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Historical apple cultivars that display high level of resistance to fire blight
19-23Views:294Following the first outbreak of fire blight caused by the bacterium Erwinia amylovora (Burrill) Winslow et al. in Hungary, we have started research with the aim to screen domestic gene sources, mostly historical Hungarian apple cultivars, for disease resistance as part of an apple breeding program for multiple resistance. The present study was conducted with the aim to choose the most tolerant historical apple cultivars among 25 selected cultivars by screening their behavior towards fire blight under controlled conditions. Six cultivars were shown to be the most disease tolerant: `Batul', 'London pepin', Nemes Sóvári Alma', `Sikulai', `Szemes alma', Wilmos renet'. We evaluated these cultivars by investigating both morphological- characteristics under original environmental circumstances and fruit quality. The cultivars had a remarkable degree of fire blight resistance compared to the control cultivars. These were not competitive with the commercially grown 'Jonathan M40' during cultivar tests but on the basis of certain characteristics they could serve as genetic sources for breeding new varieties.
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Examination of valuable ingredients of some wild fruits
71-74.Views:369A possible way of the development of Hungarian agriculture is the selection and growing of new fruit species and varieties featuring special qualities, with high biological nutritive and health protecting properties due to their natural composition. A reserve for such new fruits is the native dendroflora, e.g. those wild-growing trees and shrubs of Hungary, which bear edible fruits. The publication is giving a summary of chemical analyses done on the fruits of the plants listed below. The research team on the project started the work in 2001 with
woody species (genera) as follows:
- common elder (Sambucus nigra) clone named Szcs-1, Szcs-2, Szcs-3, Szcs-4, Szcs-5, SzcsK-1, SzcsK-2
- dog rose (Rosa canina) types: clones named Sz-1, Sz-2, Sz-3, Sz-4 and Sz-5 .
- native rowans: Sorbus dacica, S.rotundifolia, S. degenu, S.bakonyensis cv. Fánivölgy
- hawthorns: Crataegus monogyna, C. orientalis,C..v lavallei.
- cornel cherry: Cornus mas cv. Császló.
The present paper is reporting on the content of the following compounds in the fruits: dry matter (refractometric values), total acid content, ascorbic acid, 13-carotene, pectin, minerals and carbohydrates. The results have shown that these wild fruits have excellent composition. Besides their curative effects, their content of minerals, ascorbic acid and 13-carotene has surpassed that of the traditional fruits. These fruits are rich in ascorbic acid, 13-carotene and pectin. The high content of the above-listed, biologically active compounds makes the new wild fruits studied suitable for the preparation (and later: mass-production) of special curative and exclusive products.
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The role of rejuvenated and adult forms of English oak (Quercus robur) in in vitro cultures
81-83.Views:311In vitro plant material of clones (Q. robur) NL 100 A (adult) and NL 100 R (rejuvenated) received from Germany (A. Meier-Dinkel, 1995) were used in these experiments. WPM medium was used for the multiplication phase. Plantlets were subcultured monthly. Differences in quality and colour of the adult and rejuvenated cultures induced us to follow and compare the changes of mineral- and chlorophyll content and dry weight during the propagation phase. Mineral and chlorophyll content as well as dry weight were measured weekly on three samples during the subculture period.
In the case of propagation rates we stated, they were similar around the year, but both clones had a high peak in April. Examining the cation-content, we detected that, the plantlets had a highest quantity of several elements during the 2nd and 3rd week of subculture. The iron content was the highest in the 1st week and after that it decreased continuously. It is supposed, that the content of iron is not enough in the media. The chlorophyll content of the rejuvenated clone was higher than that of the adult one.
In the rooting experiments it was stated that, after one-week cold treatment the rooting ability was the best.
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Effects of methyl jasmonate, salicylic acid and phenylalanine on aloe emodin and aloin in diploid and tetraploid Aloe barbadensis
Views:438Aloe vera is one of the most famous medicinal plants. Aloin and aloe emodin are the most important active compounds in this plant. The purpose of this research was the comparison of aloin and aloe emodin production after the elicitation by methyl jasmonate, salicylic acid, and phenylalanine in diploid and tetraploid Aloe vera plants in greenhouse conditions. The plants were treated with the concentrations of 25, 50, and 100 µM. The amounts of aloin and aloe emodin were determined 24 and 48 hours after application of the treatment. HPLC analysis showed that the leaves of the control diploid plants (without applying elicitors) had more aloin (1.20 fold) and aloe emodin (1.14 fold) than the control tetraploid plants. The maximum concentration of aloin (1.15 ± 0.07 µg mg-1 dry weight) was obtained after the elicitation by 25 µM methyl jasmonate, 24 hours after treatment, in diploid plants) 6.36 fold compared to the control (0.18 µg mg-1 dry weight (. In addition, the maximum concentration of aloe emodin (0.28 µg mg-1 dry weight) was obtained after the elicitation by 25 µM salicylic acid, 24 hours after treatment, in diploid plants) 6.18 fold compared to the control (0.04 µg mg-1 dry weight)). The long-term effect of three studied elicitors (after 240 days) on plant health and survival was also studied. This investigation showed that only methyl jasmonate at a concentration of 100 µM was resulted in the death of Aloe vera plants.
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Apple powdery mildew caused by Podosphaera leucotricha: some aspects of biology
19-23.Views:576Apple powdery mildew (Podoshphaera leucorticha) occurs wherever apples are grown. One of the most important fungal disease of apple which causing severe econimic loss on susceptible apple cultivars. Biology of the pathogen is widely investigated all over the world in the past 100 years. In this review, a summary from this enormous research is made for biology of apple powdery mildew in the following aspects: geographical distribution, morphology, taxonomy of the causal agent, symptoms, host susceptibility, resistance durability and disease cycle.
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Growing greenhouse cut flower in hydro-culture
37-39.Views:286The importance of hydro-cultural growing is significantly increasing.We have been dealing with the hydro-cultural growing of cut flowers at the Department of Ornamental Plant Growing and Maintenance of Gardens at the College Faculty of Horticulture at Kecskemét College since 1988.We started our experiments by growing carnation in growing establishment without soil then we introduced other species of cut flowers and potted ornamental plants into our research work.
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Colour stability of the flowers in some modern rose varieties in Hungary
61-66.Views:374A variety trial has been carried out to study the colour changing — colour stability of the flowers of some modern roses, especially of Hungarian varieties. 100 floribundas and polyanthas were observed. Colours of the petal surface were examined at three fenological stages of the flowers: at the bud, at the young open flowers and at the aged open flower stages. The colour difference was described in HLS standard. Our work shows that valuable Hungarian varieties can be found in both studied classes, but more floribundas bred in Hungary had good colour stability than polyanthas. The best Hungarian floribundas were 'Pest' and 'Remenyik Sándor emléke', although 'Regen', `Szent Lász1ó emléke' and 'Szabó Dezső emléke' were quite good. The best Western-European floribunda rose was 'Perneille Poulsen'. Their colour stability was reliable in different situations. Colour changing of the two best varieties was almost unperceivable from the bud to the young petals and from the young to the aged petals. In the polyantha class there was only one perfect variety: the Czech `Cs1 Cerveny Kriz'. The 'Fairy Damsel' was almost excellent as well. The best Hungarian polyanthas were `Domokos Pál Péter emléke' and 'Savaria'.
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Dynamic economic analysis of greenhouse pepper production on rockwool on a family farm
43-47.Views:238The major part of the pepper growing farms in Hungary operate as family enterprises with areas varying between 1,000 and 3,000 m2. As a result of the small size, their profitability is greatly dependant on the technological level and market circumstances. Most of these farms are characteristically affected by the lack of capital, therefore, they are unable to implement any further developments with their own forces. Greenhouse pepper production on rockwool had already been subjected to analysis earlier in our research, however, those calculations were directed solely at the profitability and efficiency of production. Based on the data from 2004 in 2005 yet another and more profound analysis was set as the objective. Besides the methods already applied before, several dynamic indicators have been introduced which could also be useful for practical applications. The graphs can permit growers to monitor the temporal distribution of the costs incurred and revenues earned during production. Thereby it is easier to plan the costs and more simple to distribute them more rationally over the production period. Our experiences suggest that this sort of analytical method is applicable only in cases where a very careful and precise collection of data is ensured and the results obtained can not be generalised as being valid only for the single farm analysed. Experiences and results, however, make us consider the dynamic economic analysis as being very useful both for beginners and practicing horticulturists.
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Role of nutrient supply in yield increase and quality improvement of spice pepper
83-86.Views:217Hungarian spice pepper powder is a unique product, a real hungaricum with its flavour and aroma compounds and seasoning effect. Its competitiveness with foreign spice peppers is ensured by its high biological value deriving from the specially Hungarian production and processing technology. Besides the traditional and highly manual labour intensive processing technology, there are some modern industrial technologies as well, where high quality can be guaranteed only by producing excellent base material (raw pepper pods). This is the reason which necessitates the rational development of the elements of the production technology, such as nutrient supply. Our objective was to offer a contribution to this aim by our trials in plant nutrition.
Experiments on the nutrient supply of spice pepper were set up in the 2003 growing season in order to decide whether yields and fruit composition parameters of pepper could be increased by means of increased K fertiliser doses with lower N:K ratios. Several forms of potassium were used, as well as applying microelement top dressings in the single treatments. It was found that the increase of N:K ratio from I:1 to 1:6 did not increase yields, but resulted in higher pigment and dry matter content. Microelement top dressing had a yield increasing effect at each N:K ratio. Higher potassium doses did not accelerate ripening.
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Investigation of the in vitro regeneration of mericlones in the caribe variety of carnation
87-89.Views:234In vitro culture conditions were experimented for the relatively sensitive, but very esthaetic "Caribe" variety of carnation with uniformly dark violet flowers. Regeneration of new plants from shoot apex meristems can be significantly improved by the combined addition of very low amounts of indolebutiric acid, benzyladenine and gibberelic acid, dissolved in the Murashige-Skoog nutrient medium. Callus formation as a prerequisite for the induction of somaclonal variability can be achieved successfully with certain molar ratios between 2,4-dichlorophenoxyacetic acid and benzyladenine. Acclimation of the obtained mericlones to the ex vitro conditions was also evaluated.
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Contributions to the resumption of growth in ecodormant buds of apple
35-40.Views:268The resumption of development in ecodormant buds in terms of establishing a functional vascular connection between the inflorescence primordia and spur tissues in apple trees was investigated. Differentiation of the xylem elements could be observed first in the pedicel of the flower primordium, in the middle of January. Much later (at the beginning of April) there were mature xylem vessels in the wall of the receptacle and, merely a procambial strand for the ovule primordium which was at this time an undifferentiated protrusion of meristematic cells, only. As for phenological development of buds incubated at a temperature of 20 °C, it was the slowest in buds sampled in January, faster in buds sampled in the middle of February and, buds from the middle of March responded very quickly. The function of temperatures needed both for xylem differentiation and for the flower primordium to achieve maturity is pointed out. The nature of frost damage in vessel elements, as well the relationship between chilling requirement and growth features of apple cultivars will be discussed.
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Linking bark anatomy to Eucalyptus Physiological Disorder (EPD) in commercial clones
73-87.Views:442Abiotic stresses trigger the Eucalyptus Physiological Disorder (EPD) which poses a threat to planted and native stands. This research seeks links between eucalyptus bark histological features and EPD, in which the descriptive bark anatomy and histochemistry are approached. Barks from 5-year eucalyptus trees, from commercial clones of E. grandis, E. urophylla and its hybrids, were collected at breast height (DBH), and 50% and 75% of the commercial height, and evaluated. The eucalyptus bark consisted of a periderm (or rhytidome) and a secondary phloem with conspicuous solitary sieve tube elements (STE). The outer bark revealed a secondary phloem with collapsed STE, whereas its inner counterpart displayed non-collapsed STEs. A region crowded with calcium oxalate (CaOx) crystals in axial parenchyma, covering the non-collapsed and partially overlapped collapsed secondary phloem, was observed. Eucalyptus barks exhibited similar anatomical organization at DBH, 50% and 75% of the commercial height, irrespective of expected EPD phenotype or scores. Notwithstanding, there are qualitative differences that are associated with the proportion of non-collapsed phloem and phloem with crystals, which were higher in the tolerant clones and in trees with score 0. The more resistant clones or samples with lower EPD scores exhibited a higher proportion of the regions of living phloem, phloem with CaOx crystals, and non-collapsed phloem. These results support the hypothesis that an increased proportion of STE collapse will occur concurrently with elevated EPD scores and are the basis for an ongoing histometric approach.
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Effect of the terroir and the harvest time on the Kékfrankos grape berries sensorial properties in Eger (Hungary)
87-92.Views:335In this research the effects of the production site “terroir” and the harvest time on the most important organoleptic properties of the berries of Kékfrankos (Blaufrankish) grape variety were observed. The follow-up of the maturation process in a given parcel can effectively supplemented by the results of sensorial evaluations of the grape berries. In most cases, the tasters found significant differences in sensorial characteristics of the berries due to the harvest time, although the trends of each parcels were different. Significant differences were frequently found in the result of the observed terroirs.
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Climate change effect on sour cherry production in Central Hungary
61-66.Views:295Consideration of the soil and climatic conditions and their expected changes is very important in planning of new orchards. This research focuses on the expected climatic conditions and opportunities of sour cherry production in Central Hungary in the periods of 2021–2050 and 2071–2100, compared to the base period of 1961–1990. Results show that in the near future the expected changes are mostly favourable for sour cherry production, the utility values are increasing in most of the subregions. By the end of the century the utility values show further increase in the formerly colder subregions. However, in some subregions the utility values slightly decrease because of the appearance of the extreme dry and warm year types, though their values remain at the level of the base period or even higher.
