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Investigation of the Relationship between the SO2 Production of Different Yeast Strains and Thiamine Concentration
57-62.Views:162Our general conclusion was that the thiamine amounts of 0,6 and 3,0 mg/1 added prior to wine fermentation, resulted in higher free-S02 level in comparison to the control. Furthermore, among the yeast strains examined in our experiments, Uvaferm BC strain produced the highest free-S02 content under the conditions mentioned before. As regards the dynamics of reductone and SO, production, the concentration of the previous one was practically identical in the middle and at the end of the fermentation while the SO2 content — both free- and total — was lower at the end. Thiamine addition did not cause any difference in the organoleptic properties of wines as proved by sensory analysis.
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Comparative economic analysis of superintensive and intensive apple orchards
45-48.Views:245The objective of thsi study was to compare economic aspects of superintensive and intensive apple production.
According to our results, conclusions and recommendations regarding the establishment of superintensive orchards are the followings: A yield of 60 tons per hectare in the average of the mature years provide a quite late payback, in this way yield losses should be avoided in the plantation of such a huge capital requirement. When yield losses happen, which cannot be avoided or may be avoided only in a limited way, up to 65 to 70 tons per hectare yield should be reached even in good years, in order to yield the average 60 tons per hectare in the long run. In this case yield losses from production technological mistakes must not be arisen. Inputs and professional expertise should be used in a maximum way in order to reach yields ensuring profitable production. Investment subsidies may ensure safer return. The return of a superintensive orchard from totally own sources may be risky under the domestic marketing conditions and selling prices, it is strongly uncertain. -
Flower constancy of honeybees (Apis mellifera L.) to blooming pear plantations
81-85.Views:257Studies were made on the composition of pollen loads of honeybees captured at the flowers of blooming pear trees in pear plantations. Also the foraging behaviour of honeybees was observed. Overwhelming majority of honeybees visiting the flowers of 13 pear cultivars in 1996 were pollen gatherers (95.6 per cent). Proportion of pure nectar gatherers was as low as some 3.7 per cent and no more than 0.7 per cent performed mixed behaviour. The analysis of pollen loads of bees collected at pear flowers in blooming pear plantations showed that fidelity was as high as 89-90 per cent towards pear, higher than for another fruit species in other studies. Even those plant species that are regarded to be strong competitors of blooming fruit trees in the literature (Taraxacum officinale, Stellaria media, Lamium purpureum) were scarcely represented in the loads. Accordingly, honeybees can be much more important and more effective pollinating agents of pear cultivars than generally believed.
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Effects of methyl jasmonate, salicylic acid and phenylalanine on aloe emodin and aloin in diploid and tetraploid Aloe barbadensis
Views:259Aloe vera is one of the most famous medicinal plants. Aloin and aloe emodin are the most important active compounds in this plant. The purpose of this research was the comparison of aloin and aloe emodin production after the elicitation by methyl jasmonate, salicylic acid, and phenylalanine in diploid and tetraploid Aloe vera plants in greenhouse conditions. The plants were treated with the concentrations of 25, 50, and 100 µM. The amounts of aloin and aloe emodin were determined 24 and 48 hours after application of the treatment. HPLC analysis showed that the leaves of the control diploid plants (without applying elicitors) had more aloin (1.20 fold) and aloe emodin (1.14 fold) than the control tetraploid plants. The maximum concentration of aloin (1.15 ± 0.07 µg mg-1 dry weight) was obtained after the elicitation by 25 µM methyl jasmonate, 24 hours after treatment, in diploid plants) 6.36 fold compared to the control (0.18 µg mg-1 dry weight (. In addition, the maximum concentration of aloe emodin (0.28 µg mg-1 dry weight) was obtained after the elicitation by 25 µM salicylic acid, 24 hours after treatment, in diploid plants) 6.18 fold compared to the control (0.04 µg mg-1 dry weight)). The long-term effect of three studied elicitors (after 240 days) on plant health and survival was also studied. This investigation showed that only methyl jasmonate at a concentration of 100 µM was resulted in the death of Aloe vera plants.
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Economic figures of apple production at national level of Hungary
103-105.Views:336Apple is the most important fruit in Hungary, despite of that, it is a paradox that during the last years, apple growing was the most unsuccessful enterprise among the prosperous ones in the country. The real cause of regression was unequivocally the low price, which could be obtained and the inadequate structure of apple varieties. A heavy problem is still the overweight of the quality doomed to supplying the processing industry instead to the fresh consumption. In order to improve the conditions, we aimed the economic analysis of the five year long period, 2005-2009, to trace the changes involving values and costs of production, income and all conditions combined with this activity. The data presented in the paper are means of a heterogeneous population of values, which cannot be utilised for immediate use in management, but they may reveal the challenges and tendencies of that branch in economics.
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Antioxidant capacity, total phenolics and mineral element contents in fruits of Hungarian sour cherry cultivars
59-64.Views:509Several epidemiological studies revealed that the consumption of antioxidant compounds and the risk of atherosclerosis, increased blood pressure or cancer are inversely proportional. Fruits of sour cherry contain a wide range of antioxidant compounds including melatonin, perillyl alcohol, ellagic acid, several flavonoids, polyphenolics, and anthocyanins. This study was carried out to survey the antioxidant power and mineral element content of seven commercial sour cherry cultivars and three cultivar candidates and to assess the influence of some external conditions on fruits' functional properties. Our analysis revealed nearly 5- and 2-fold differences between the lowest and highest antioxidant capacities and total phenolics content, respectively. Some cultivars (`Kántorjánosi' and ‘Újfehértói fürtös') and cultivar candidates (D, 'Petri' and 'Éva') showed outstanding antioxidant capacity and total phenolic content; in addition, mineral element content in fruits of the ‘Újfehértói fürtös' cultivar was also favourable. Redox parameters of fruits were influenced by the cultivation plot or fruit positions within the canopy in about half of the cultivars tested. Genetic background of cultivars forms the decisive factor in determining fruits' antioxidant capacity, although external factors may have also sizeable modifying effects. Enhanced functional properties of the fruit may also be further increased through breeding programs since considerable variation exists within the tested germplasm.
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Comparative analysis of Hungarian Matricaria recutita (L.) Rausch. populations
81-85.Views:239Matricaria recutita L. is a traditional medicinal plant in Hungary and its drug is known as „Hungaricum", world-wide. Plant samples and seeds were collected from 12 different habitats of three significant geographical regions of Hungary in 2001. Morphological, production biological and chemical properties of samples were examined. In relation to the morphological characteristics, a negative correlation (r= -0.75) could be observed between the average height of the plants (height of flowering shoots) and the pH value of the soil. According to the composition of the essential oil, the populations accumulating typically chamasulene (10-20%), a-bisabolol (30-50%) or bisabolol-oxid (30-50%) could be completely distinguished. Concerning the flavonoid composition the quantity of apigenin-7-glucoside was outstanding in the populations originating from the Great Hungarian Plain, it has reached the concentration of 1.8-2.8 mg/g, while the samples collected in Transdanubia could be characterised by much lower level of apigenin-7-glucoside (around 1.5 mg/g).
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Cost-effective plantlet production and wintering method of virginia fanpetals (Sida hermaphrodita L. Rusby)
137-141.Views:222The main goal of this research was to work out programmable, cost-effective and industrial scale technologies of mass propagation from the seeds of rootstock nurseries of undomesticated American populations of Sidahermaphrodita. During our previou`s seed treatment experiments, it was concluded that around 60% of the Virginia fanpetalsseeds collected during the four cropyears can be considered as high quality, infection-free, normally imbibing and germinating seeds (Kurucz et al., 2013a,b). The experiments performed with the nurse-in-tray method developed by us showed that the summer-autumn nurse-in-tray plantlet production and unprotected wintering of Virginia fanpetals with properly pre-treated and fractioned seeds is a promising new method. No weeds appear between the plants, but only on the side of the cases during plantlet production. The investment cost of the method is minimal. There are no heating costs and this phytotechnique can be easily and properly mechanised. Plantlet production can be performed near the large-scale plots. After exploring the root and shoot system, it was concluded that the nurse-in-tray method is suitable for producing plantlets with hardened and strong roots. Scheduled plantlets can be produced in an industrial scale volume by the time of early spring (March) plantlet planting. The excavateof plantlets can be flexibly adjusted to the needs; they may even grow in the plantlet cases for a whole year. We think that these innovative plantlet production and wintering methods which are suitable for large-scale use will make Virginia fanpetals a proper feedstock for the constant supply of the Biomass Supply Chain both in Hungary and in European countries which are in the same climate zone. The comparative analysis of the costs of this procedure calls for further research.
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Comparison of apples from organic and integrated farming
15-18.Views:326Prima’, ‘Gala’, ‘Remo’, ‘Topáz’, ‘Idared’, ‘Releika’, ‘Resi’, ‘Rubinola’, ‘Rajka’, ‘Rewena’ and ‘Florina’ apple cultivars, both from organic and integrated farming, from Pallag and Újfehértó, were compared. Average size, weight, soluble solids, titratable acidity, total polyphenols, free radical scavenging capacity expressed as Trolox equivalent (TEAC), copper and zinc were determined at harvest and after cool storage. Organic apples were more acidic, while integrated fruits had mostly higher copper and zinc content. Total polyphenols and TEAC values did not show a significant difference as a function of farming technology. A principal component analysis shows the separation of provenances as well as stored and fresh apples. Results are considered as preliminary.
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Post-storage sensory quality of apple varieties
54-57.Views:129The changing situation of apple production in Hungary has given rise to a great demand for new varieties. Besides bearing capacity, fruit quality and suitability for new training systems, consumer preference is one of the grower's main considerations. Growers need reliable information in order to choose the appropriate variety, so consumer tests have become essential. Altogether, 1 I traditional and new varieties were involved in this project. In the first part of the experiment, panelists ranked the coded samples according to their appearance. In the second part, apples were cut into unpeeled slices and the samples were also coded. The assessors were asked to taste them and rank them again. The sensory tests were held after three different storage periods.
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Participatory evaluation of tomato (Lycopersicon esculentum Mill.) varieties under irrigation conditions at Abergelle district, Eastern Amhara
17-22Views:111Participatory on-farm evaluation of improved tomato varieties (‘Melka salsa’, 'Roma VF' and 'Kochero’) against the local tomato variety was carried out during the 2019/2020 growing season involving six farmers of Abergelle district, Eastern Amhara. The objective of the experiment was to assess the performance of different tomato varieties for the farmers, then to collect their feedback. Based on the actual and farmers’ preference data, the analysis underscores the better performance of improved tomato varieties over local varieties by most yield-related attributes. Average marketable fruit yields of ‘Melka salsa’, ‘Roma VF’, ‘Kochero’, and the local variety were 4.62, 3.88, 3.64, and 3.10 ton ha-1, respectively. The improved varieties thus had a yield advantage of 72.38%, 37.14%, and 25.72% over the local variety in that order. Among improved tomato varieties, ‘Melka salsa' provided the highest fruit yield on top of owing the highest score of overall preference attributes rank. Scale-wide diffusion of ‘Melka salsa’ tomato variety is therefore suggested for similar and potential agro-ecologies. Biological scientists also should take farmers’ preference attributes and feedback as a backup for future breeding and adaptation studies on tomato varieties.
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Technologies developed to avoid frost damages caused by late frost during bloom in the fruit growing regions of Siófok and Debrecen
99-105.Views:414The aim of the study was to fi nd out which of the methods used to avoid damages of late frosts would be the most effective for the fruit growing practice. Three technologies have been tested in the regions of Siófok and Debrecen-Pallag. The antifrost irrigation proved to be the most advantageous. For that purpose microjet sprayers are used, which are thrifty and do not need for that purpose large containers. With the aim to secure an even distribution of water, the sprayers are distributed on three levels: above and inside of the crown as well as on the level of trunks. On a large scale, a single microjet above the crown level would be suffi cient. By means of a detailed analysis served to set the optimum intervals between spraying phases: with each 15, 10, 5 and 3 minutes during half a minute. The synchronous presence of water and ice below the freezing point, the released freezing heat plus the water used much above the freezing point (9–10 °C) altogether maintains the temperature above around 0°C near the fl owers or growing fruitlets, meanwhile, the surrounding air cools down to –8 °C. The effectiveness of the generally used anti-frost would be increased substantially by the former application of cooling irrigation, which delays the blooming date. The Frostbuster represents a new technology developed for the same purpose of frost defence. It uses butane burning and produces high (80-90°C) temperature with a strong blast of air. On the protected area sensors have been distributed for measuring temperature and relative humidity. The means of the measurements proved a rise of temperature, which was suffi cient to save the fruit trees until the difference is less than –3–3.5 °C. Our analyses stated that paraffi n candles avert the frost until –4 °C. Its success depends largely on the intensity of air movements. A small wind would be enough to frustrate the effect. The results show clearly the utility of Frostbuster and paraffi n candle combined is approved.
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Analysis of amino nitrogen content affecting fermentation and wine quality
13-16.Views:186A suitable amount of nitrogen source is needed for the optimal process of alcoholic fermentation. The professional literature mentions the complexity of nitrogen compounds exploitable for yeast-plants as an assimilable or immediately assumable nitrogen source. Former experiments proved that in case of low nitrogen concentration , yeast-plants produced a bigger quantity of hydrogen sulphide. According to the findings above, it is not indifferent when and how we complement the nitrogen content of the must. The aim of our present work was to elaborate such a quick, easily applicable method that can be used for routinish measurements in the viticultural practice.
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Comparison of light yellow fleshed pepper varieties grown on rockwool under unheated forcing conditions
108-110.Views:116Experiments are going on all over the world assisting the joint effort of researchers and practicing specialists to identify the methods which can help either in the reduction of production costs or in the increase of yields.
The task of the growers is to make profitable use of the forcing facilities and to satisfy market demands at an acceptable price by means of improving production technology and applying new scientific, technological and technical information.
For the last few years, rockwool based forcing has been gaining in importance. The subject of our scientific work was the analysis of an important question of this technological variant, the selection of the variety. Besides, we also tried to identify the most suitable pruning technology for the varieties studied.
In Hungary, the highest demand is commonly known to be for the light yellow fleshed varieties which are suitable for stuffed dishes. In the future, due to their special quality and appearance, as well as to the Hungaricum character, they could become important export goods on the European Union market. It was within this variety type that comparison between varieties already common in production (HO F1, HRF F1, Danubia F1) was carried out, trying to get an answer to the question which of the three varieties could be produced with the greatest success. Considering the quantitative and qualitative indicators, it was H6 F1 that proved the best out of the three varieties tested under unheated forcing on rockwool. It excelled the other two varieties both in quality and in average fruit weight, preserving this advantage until the end of the growing period.
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Anatomical study of the bud union in „Chip" and „T" budded 'Jonagold' apple trees on MM 106 rootstock
27-29.Views:422The traditional methods for vegetative propagation of apple and its varieties are the T-budding, and the winter grafting, but this latter way is a difficult and expensive procedure.
In our experiment carried out in the Fruit Tree Nursery Soroksár, the healing process of chip- and T-budded apple trees 'Jonagold' on MM 106 rootstock was studied.
The budding (T- and Chip-) was made in the first week of August, samples for microscope examination were taken monthly after this time until leaf fall.
The investigated part of plants was made soft with 48 % HF (hydrogenfluoride), then cross and longitudinal section were made and examined by microscope.
Based on analysis of microscope pictures in case of Chip-budding, it was established, that development had started quickly after budding on the rootstock and scion too. But the callus originated almost entirely from the rootstock tissue as new parenchyma cells fills the gap between the two components of graft (scion and stock), becoming interlocked and allowing for some passage of water and nutrients between the stock and the scion. This quantity of callus in case of T budding was under the scion buds larger, than the Chip-budded unions, where the thickness of callus mass is uniformly thick round the chip. The large mass of callus pushes the scion bud outwards from the shoot axis, which later results in a larger shoot-curvature above the bud union.
Following this process on the Chip-budding it can be observed also, that a continuity of the cambium is established between bud and rootstock. Then the newly formed cambium started typical cambial activity, forming new xylem and phloem.
Later the callus begins to lignify, and it is completed within about 3 months after budding.
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Economics of sea buckthorn production and processing in Hungary
21-25.Views:291This study focuses on the business management-related advantages and disadvantages of sea buckthorn production and processing based on economic analyses. It is the main objective of the authors to identify the expected economic findings in a high standard plantation with different average yields. A deterministic model calculation was performed on the basis of technological processes, using the primary data collected from enterprises dealing with sea buckthorn production. The calculation is based on the assumption of a 10 hectare plantation with intensive production technology (high soil quality (golden crown value: 32 GC per ha), irrigation, high plant density per hectare). The cost and income relations and the long-term return of the plantation were examined in the case of different average yields (12 t ha-1, 18 t ha-1 and 24 t ha-1). Under the economic circumstances of 2016, the planting cost of an intensive plantation is around 4-4.1 million HUF ha-1. In the years following the fruit-bearing stage, direct production costs are between 2.5-3.9 million HUF ha-1, depending on the given average yield. On the contrary, 5.6-11.1 million HUF ha-1 revenue can be reached based on the current market prices, resulting in a gross margin of 3.1-7.1 million HUF ha-1. Under the modelled circumstances, return is realised on the plantation’s costs in 6-8 years. The net present value (NPVr=3.24%) calculated for the 15-year-long life cycle of the 10-hectare plantation is between 151-466 million HUF, while the internal rate of return (IRR) is between 23-45%. From the business management aspect, the advantage of sea buckthorn production is that it provides better income and return at a planting cost which is similar to that of other small fruits and berries. At the same time, the disadvantage of sea buckthorn production is the fact that yields are harvested every two years due to the technological characteristics of harvesting. The negative impact of this bi-yearly yield on liquidity can be eliminated with the so-called delayed planting.
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Sunburn assessment: A critical appraisal of methods and techniques for characterizing the damage to apple fruit
7-14.Views:405Many methods and techniques have been introduced for measuring alterations in the fruit and in its surrounding environment related to sunburn incidence. The research objectives, fruit materials and the environment to be evaluated dictate the methods to follow. These procedures are either non-destructive and involve techniques that allow us to track the course of sunburn development and related environmental parameters, or destructive and involve the removal of fruit from the tree for field/laboratory measurements. Techniques employed can be used for pre-symptomatic monitoring (before symptoms become visible) or characterizing the symptoms already present. The principles behind the measurements and their usefulness for sunburn assessments are discussed and critically evaluated in this review paper. Descriptions and evaluations of the methods and techniques were made in the following groups: 1. Thermal measurements; 2. Visual assessments; 3. Fruit quality measurements; 4. Measurements of physiological and biochemical alterations; and 5. Practical evaluation of sunburn damage. Thermal measurements involve methods tracking the ambient temperature and fruit surface temperature, and their relation to sunburn formation. Visual assessments cover all measuring techniques (skin color, chlorophyll fluorescence, radiation reflection, electron microscopy) that are able to detect changes on/in the fruit skin related to sunburn formation. Fruit quality measurements are used to point out differences in qualities (soluble solids, firmness, titratable acidity, and water content) between unaffected and sunburned areas of the fruit. The measurements of physiological and biochemical alterations (gas exchange, pigment analysis, enzyme activity, gene expression) give us a better insight to the mechanism of sunburn formation. Practical evaluations involve many procedures that are used by scientists to characterize the susceptibility of cultivars, evaluate protection technology, etc. For this purpose, the following methods are in use: expressing the percentage of the total fruit surface area affected by sunburn or the percentage of the total number of fruits damaged on the tree, or even a scale based on the severity of the symptoms occurred. All assessing methods and techniques described here have their pros and cons as well as their specific applicability, therefore any of these cannot be favored to use exclusively for assessing sunburn incidence. The combination of these techniques will be the best choice to meet a given research objective perfectly.
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The antioxidant capacity of sea buckthorn (Hippophae rhamnoides L.) berries depends on the genotype and harvest time
27-29.Views:329Berries of sea buckthorn (Hippophae rhamnoides L.) are characterized by increasing popularity due to their presumable healtheffects. The aim of this study was to compare the antioxidant capacity and total polyphenolic content in the berries of six Hungarian grown sea buckthorn genotypes and characterize the genetic variability in this trait. The harvest time of sea buckthorn berries affects the antioxidant capacity and total phenolic contents in berries of three popular cultivars of German origin. Berries harvested in October had higher antioxidant capacity compared with those harvested one month later. The extent of the difference was genotype-specific. Our analysis revealed a nearly 3-fold difference between the lowest and highest antioxidant capacities of the 6 tested genotypes with ‘Leikora’ showing the highest ferric reducing antioxidant power and total phenolic content. The TEAC values ranged between 1.76 and 3.13 mmol Trolox/100g fresh weight with Pető 1 and ‘Frugana’ having the highest values. The results presented in this study demonstrated that Hippophae rhamnoides berries possess in vitro antioxidant activity strongly determined by genotype but also influenced by harvest time.
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Production and marketing of medicinal and aromatic plants
63-75.Views:158The utilisation of medicinal and aromatic plants is growing continuously, which is motivated by both psychological and rational factors. Species belonging to this special group of plants were produced in the territory of Hungary many centuries back. However, the intensification of the production started in the first years of 20 th century, only, and in spite of many political and economical contradictions the medicinal and aromatic plant sector became a successful part of Hungarian Agriculture. Some of the national products had been accepted as a special Hungarian product ("Hungaricum") evaluated as most respectful in the world market. To stabilise, even to develop the production of medicinal and aromatic plants in Hungary the understanding of the actual world situation, especially the forthcoming EU conditions, became essential. Based on the analysis of the situation market and economy of medicinal plant production, a new strategy had to be developed. The medicinal and aromatic plant sector requires a special development program, which should be supported by Hungarian authorities, focusing on the subjects, which are as follows:
- development of "quasi" EU compatible Hungarian regions specialised for medicinal plant production,
- development of biological background, technology as well as the quality assurance system of the production,
- EU compatible regulation and subvention system of the sector, including processing, production and marketing of raw and phytomed ici nes,
- development of new marketing strategy in both national and international relations.
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Comparative analysis of sour cherry cultivars on their ecological and biological indicators
7-28.Views:363Sour cherries developed in the northern hemisphere, an alloploid hybrid of dwarf sour cherries (Prunus fruticosa) and bird cherries (P. avium), born in the confluence of the two species. However, the ecological and, above all, cold tolerance of the ancestor of cultivated sour cherries is higher than that of wild cherries (De Candolle, 1894; Rehder, 1954; Terpó, 1974; Iezzoni et al., 1991; Faust & Surányi, 1997). The cultivation limits are in the northern hemisphere 38-44. degree. The Carpathian Basin, the Balkans and Asia Minor are considered to be the main birthplaces for sour cherries. The genetic and morphological diversity of sour cherries is greater than that of the basic species (Iezzoni et al. 1991; Faust & Surányi, 1997). In the study, 472 sour cherry cultivars were compared based on 7 relative ecological indicators and 3 biological values. Compared to other Prunus species, we mostly found less variability in sour cherries - not counting their salt tolerance (SB). The partial similarity between open pollination (OP), frost tolerance (FR) and disease resistance (DR) - partly true in terms of varieties, but also reflected the effects of purposeful breeding and selection. The cultivars together - in comparison, showed balance, but in the highlighting, the differences of the 3 cultivar groups became significant. Indeed, the differences between the species of the former Hungarian cultural flora are clearly different (Surányi, 2004), which is also the case when comparing a large number of apricot (Surányi, 2014), plum (Surányi, 2015) and peach (Surányi, 2020) varieties.
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Prevention and protection technologies – How do the growers get ready for climate change?
67-69.Views:400Within the sector of fruit growing, climate change related tasks cover a rather wide range of activities. According to what is claimed by the literature, all decisions impacting the sector should be made conformably with climate change in order to advance an increase in yield security. This, regardless of the impacts of climate change, is also one of the key questions in fruit growing. Regarding protection against extreme weather events, in addition to technological and technical elements, the level of importance assumed by farmers for the abovementioned protection techniques as well as the type and extent they intend to use of this in practice are also worth of studying. This ongoing research beginning in 2009 mainly focuses on studying the opinions of fruit farmers making up the target group for this analysis. The questionnaire survey primarily intends to study their knowledge on the definition of climate change as reactivity to unfavourable weather events occurring in the growing. The study aims at providing a realistic view on the fruit-farmers’ knowledge on climate change and on how technological elements, new technical solutions applicable to mitigate damage are used during production.
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Ecological drought resistance and adaptability of apple varieties
113-122.Views:446For adequate yields in apple plantations, during the long growing period of the fruit primordia, one of the decisive factors is water supply. Indicators of stress are valuable signs for the diagnosis of drought and necessity of watering, i.e. planning the irrigation of plantations. The aim of the present study was to find reliable signs of water stress on apple trees and at the same time conclude on the drought tolerance of different varieties. The plantation of apple varieties grafted on various stocks and cultivated according to different systems (irrigated, non irrigated, integrate and biological) has been examined continuously by leaf analysis. Along the period of growing fruits, measurements were made in the field, then the leaf samples were analysed in the laboratory for composition of pigments, carbohydrates and antioxidants, as well as the histology of the tissues checked. Without irrigation, the mean leaf mass and the relative chlorophyll content (SPAD) of the variety Idared on M4 stocks increases beyond the values of 50 SPAD, whereas on M26 stock and integrated system, it declines. In the collection of varieties, grown according to the „integrated” system, during the development of fruits, ‘Gála’ and ‘Remo’ varieties have been affiliated to the “less susceptible” group regarding drought tolerance because of the leaf morphology, structure and content of SPAD and antioxidants. However, ‘Idared’ and ‘Jonagold’ belong to the “susceptible” group. To the same group are ranged the ‘Akane, Red Rome vanWell, Pink Lady’ varieties. Those varieties reacted to a short period of drought by increased production (content) of carbohydrates. Regarding changes of carbohydrate content ‘Greensleeves’ and ‘Ozark Gold’ varieties belong to the moderately water dependent (requiring) group. In the field, SPAD-tests facilitated the measurement of nutrient-uptake and incorporation, which is proved by the tight correlation between the data of SPAD and the increment of leaf weight (r=0.76–r=0.88), however, this depends on the variety too. SPAD is an indicator of water supply and is related with the density of stomata, cannot used for the selection of water-exigent varieties but for (drought) tolerant ones. In integrated culture, the (drought) susceptible varieties display (water-soluble) hydrophilic and lipophilic antioxidants (ACWand ACL resp.) the quantity of which may have some role in drought tolerance.
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A critical evaluation of methods used for S-genotyping: from trees to DNA level
19-29.Views:227Fruit setting behaviour of fruit trees remains to be in the focus of plant breeders and growers. Realizing that most species (cherry, apple, pear etc.) are self-incompatible and certain cultivars are cross-incompatible, mutual fertility properties and their reliable determination are of great interest. This review gives a comprehensive description of all known S-genotyping procedures, i.e. the classical fruit set analysis after open field test crosses; pollen tube growth monitoring with fluorescent microscopy; stylar ribonuclease electrophoresis (using different types of isoelectric focusing and 2-dimension polyacrilamide gel electrophoresis); as well as the most recent polymerase chain reaction based DNA-level analyses and DNA sequencing. The review presented not only gives a compilation of the bases of the methods described but also provides a critical evaluation and a comparative characterization of their applicability.
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Detection and identification of phytoplasmas in peach based on woody indexing and molecular methods
35-41.Views:172Symptoms resembling phytoplasma disease have been observed on peach trees in a seed-source plantation of stone fruits in south Hungary quite recently. In this publication we report on the results of woody indexing of symptomatic peach trees on GF 305 indicator in the field and under greenhouse conditions as well as on molecular studies. Phytoplasma infection detected on GF 305 indicators in greenhouse and field indexing was confirmed by PCR. Nested PCR was conducted using universal primer pairs followed by group and subgroup specific primers for the second amplification. RFLP analysis of nested PCR products was performed using Rsal restriction enzyme. Based on the results of molecular studies it can be concluded that phytoplasmas, belonging to the European stone fruit yellows subgroup (16SrX-B) were identified in peach trees. Further studies on symptomatic peach trees originating from different parts of Hungary are in progress.
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Vineyards characteristic by using GIS and refl ectance measurements on the Nagy-Eged hill in Hungary
57-60.Views:233Our investigation was carried out in vineyards on Nagy-Eged hill in Hungary. Grapevine growing plays an important role in Eger Vine Regions. In the grapevine cultivation it becomes necessary to know the requirements of vineyards along the best geographical situation using available cultivation and examination technology to achieve better and quality produce. This study reviews applicability of the different spatial analyst techniques in vineyard especially models based on surface relief and the new technology of hyperspectral analysis. The spectral alteration was examined between different health status and leaves infected by Eriophyes vitis to defi ne spectral characteristics. Thus reflectance measurements could also support to evaluate airborne hyperspectral images with the help of the spectral library.