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Farm economic evaluation of elderberry production
57-60.Views:209In this present study the efficiency as well as the farm economic advantages and disadvantages of elderberry production are examined. Our objective is to determine the fact that under what conditions the elderberry production may be profitable regarding the present economic and market situations. Our analysis was carried out by a simulation model based on a farm-level data gathering in production enterprises. The total investment cost of an up-to-date, elderberry orchard of traditionally cultivated without irrigation is between 1000 to 1700 thousand HUF per one hectare and turning to productivity is expected within 4-5th years. These orchards are able to produce yields of 8,0 to 9,0 tons per one hectare in the average of the productive years, which makes reaching a revenue of 800 to 1000 thousand HUF possible regarding a per kg average selling price of 80 to 110 HUF. By this a net profit of 200 to 400 thousand HUF may be realized in case of a per hectare production cost of 600 to 700 thousand HUF. At the end of the lifetime of the orchard (12-15th year) an internal rate of return of 10 to 4%, an NPV of 1500 to 2000 thousand HUF per one hectare are typical in an average case, and the payback may be expected in the 6th to 8th year. From the farm economic aspect the elderberry may be considered as an extensive sector, which advantages are low capital and labour need, early recovery, good-acceptable profit on capital and cost to profit ratios, but its disadvantage is low per hectare profit comparing to intensive fruit species and orchards. In this way in general farms of capital-extensive and avoiding risks choose elderberry production.
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New evaluation method to detect physiological stress in fruit trees by airborne hyperspectral image spectroscopy
37-40.Views:163Nowadays airborne remote sensing data are increasingly used in precision agriculture. The fast space-time dependent localization of stresses in orchards, which allows for a more efficient application of horticultural technologies, could lead to improved sustainable precise management. The disadvantage of the near field multi and hyper spectroscopy is the spot sample taking, which can apply independently only for experimental survey in plantations. The traditional satellite images is optionally suitable for precision investigation because of the low spectral and ground resolution on field condition. The presented airborne hyperspectral image spectroscopy reduces above mentioned disadvantages and at the same time provides newer analyzing possibility to the user. In this paper we demonstrate the conditions of data base collection and some informative examination possibility. The estimating of the board band vegetation indices calculated from reflectance is well known in practice of the biomass stress examinations. In this method the N-dimension spectral data cube enables to calculate numerous special narrow band indexes and to evaluate maps. This paper aims at investigating the applied hyperspectral analysis for fruit tree stress detection. In our study, hyperspectral data were collected by an AISADUAL hyperspectral image spectroscopy system, with high (0,5-1,5 m) ground resolution. The research focused on determining of leaves condition in different fruit plantations in the peach orchard near Siófok. Moreover the spectral reflectance analyses could provide more information about plant condition due to changes in the absorption of incident light in the visible and near infrared range of the spectrum.
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Prospects of mango fruit powder production at farm level and its utilisation during mango off-season in Ghana
Views:275Mango (Mangifera indica) is an important fruit served to customers as fresh-cut fruit, juice, ice cream and yogurts in many fruit juice joints, restaurants and hotels in Ghana. However, the crop’s highly seasonal and perishable nature is a challenge for food processors and farmers. Preservation of the fruit in dry particulate form can serve as a substitute in off-seasons. The study sought to determine mango fruit powder production prospects at the farm level and explore its potential use as a substitute during the off-season. Farmer groups and food enterprises were therefore interviewed. The study showed that Keitt and Kent varieties were the most cultivated varieties because of market demand and high yield. However, an average annual fruit loss of 29.8% at the farm level was observed. Postharvest extension delivery service to farmers is inadequate, and where available, frequency of contact is irregular. A limited number of farmers received training in fruit processing; and few were engaged in transformational value addition activities but were willing to add value through processing into powder. Probit regression analysis showed that a unit increase in training would increase transformational farm level value addition into mango fruit powder by 22.9%. The majority of the food enterprises source fruits within Ghana while 79% experienced mango fruit shortage in the off-seasons. Only a few of the enterprises used mango fruit-based substitutes to serve customers in off-seasons. Probit regression analysis showed that a marginal increase in processors’ use of substitute significantly (p≤0.05) increased willingness to use mango fruit powder as a substitute by 47%. The study has revealed that local production of mango fruit powder could serve as a substitute to fill the seasonal gap in mango supply and also reduce post-harvest losses.
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Investment appraisal of a plantation establishment for intensive apple production
17-20.Views:163For fruits, establishing intensive apple-orchards requires the highest amount of investment cost, while the returns depend on many factors. Based on farm and bibliography data we appraised an investment in a model in some variations that are the most used in practice (100% owner's capital and 55% owner's capital +45%o subsidies). The profitability of the investment has been analysed using the methods of NPV (Net Present Value) and DPP (Dynamic Payback Period). The essence of our analysis is a sensitivity analysis with the optimistic, pessimistic and realistic combinations of the yield and the market price. Plantation establishment financed by only own (corporate's) sources turns into profitable over 7-10 years in average and favourable cases, but the opposite is the case in unfavourable circumstances. By subsidy of 45% for investments, it is highly possible to return by the fifth or sixth year after the year of establishment, but it can return by the twelfth year even in unfavourable case.
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Investment analysis of Hungarian apple-orchard and fruit storage projects
157-162.Views:219There are profound, long-term changes in world apple production and trade. The former hegemony of Europe in apple production doest not exists any more, among the most important apple exporters the emerging economies have a growing importance. The globalising apple market means new challenges of traditional producers. This is especially true for Hungary, which has been the most important apple exporter in terms of quantity thirty years ago, but now its production hardly covers the domestic demand. A necessary precondition of the modernisation is the re-construction of plantations and the cold-storage system. Analysing the economic effi ciency of apple production and cold storage, it is obvious, that a necessary precondition for the modern, competitive apple production is the availability of cold-storage facilities. Neither the apple-production, nor the cold-storage can not evaluated separately from each other. Under current Hungarian conditions there is a need for active state support for the establishment of cold-storage facilities.
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Improved evaluation method for reliable distinction of poppy (Papaver somniferum L.) cultivars
68-73.Views:164Investigating seven Hungarian standard cultivars (i.e. 'A1’, 'Kék Gemona', '13C-2`,'Kék Duna', `Kompolti M’, `Gödi N',
'Kozmosz) it was proved that for the reliable distinction between cultivars a complex analysis is necessitated. Because of the large morphophenological and production-biological plasticity of the species qualitative and quantitative features as well as alkaloid accumulation characters have to be evaluated simultaneously applying discriminant analysis.
It was proved by the investigations that some of the qualitative and quantitative characters included into the DUS evaluation procedure have slight importance for reliable distinction. As an example the majority of cultivars show high inhomogentity in such characters like the shape of stigmatic disc, the number of stigmatic lobes etc. Furthermore these characters never had been chosen for the goal of any practical selection and are modified by the weather conditions as well.
In the evaluation process the alkaloid accumulation characteristics (both qualitative and quantitative ones) have to be evaluated on a higher rank. For instance the high accumulation level of morphine in cv. 'A1' (10.5 %o), exceptionally high narcotine accumulation in 'Kék Gemona' (7.8 %o), or the low morphine accumulation level in cv. 'Kozmosz' (1.1 %o) proved to be proper characters for their distinction, alone. However, at the same time the weather conditions existing at the time of experiment have to be taken into consideration. From practical point of view, the years rich in precipitation and poor in sunshine are less suitable for distinction of cultivars selected for higher alkaloid accumulation, especially for production of codeine and thebaine characterized by a higher methylation level. Based on the example of 'Kék Gemona', the accumulation level of morphine decreases from 7.4 %o to 4.1 %o under such conditions, while thebaine and codeine disappear, practically.
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Study on the micronutrient content of soil and leaf of an organic apple orchard in Eastern Hungary
7-11.Views:162The aim of our study was to investigate the relationship between micronutrient content of soil and leaf in an organic apple orchard. The experiment was carried out at the Experimental Site of the University of Debrecen, Centre of Agricultural Sciences at Pallag in an apple cultivar collection in Eastern Hungary in 2002-2004. The soil sampling was carried out twice per year, at the beginning and the end of the vegetation period in 2002, 2003 and 2004, respectively. The micronutrient content of leaf of seven apple cultivars (Jonagold, Mutsu, Idared, Red Elstar, Egri piros, Reka, Remo) was studied according to phenological phases of apple (April — September) to investigate the dynamism of plant nutrient uptake.
It was found that results of leaf analysis correlated with the obtained results of soil. According to the data of July, manganese and copper content of leaf is in the 'favourable' but zinc is the 'low' range of nutrient supply category. Moreover, there were significant differences in the micronutrient content of leaf among cultivars. From our results it is evident that more reliable data were obtained for fruit nutrition by determining of micronutrient content of soil and plant and calculate their ratios as well.
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The effect of wear of petrol engine power brush cutters on their vibration exposure
37-44.Views:184The matter of noise and vibration exposure has an accentuated emphasis in the environmental protection policy of the European Union. In practice, the focus is on the evaluation of noise -strain. The evaluation on human beings' vibration-strain is in focus in recent years. At the Technical Department in Corvinus University of Budapest Faculty of Horticultural Sciences we evaluate in PhD training form the possible ways of alleviation and revealance of noise and vibration-strain in gardening devices and machineries. According to the latest development in gardening culture, small gardening machines are more and more widespread. There is a law in Hungary for the compulsory grass-cutting, especially on 'ragweed' (Ambrosia elatior). Along highways, public territories, parks workmen at least six months are in charge to terminate these weed-crops meanwhile they are endangered by serious harmful effects. The operators of hand-held power tools are exposed comprehensive levels of hand-arm vibration at the tool-hand interface. Many studies indicate that extended exposure to mechanical vibration can induce degeneration of the vascular and sensioneural systems in the hand called hand-arm vibration syndrome (HAVS). The precise mechanism for the initiation and development of HAVS is unclear to date. Measurement and risk assessment of hand-transmitted vibration is mostly based on the guidelines and dose-response relationship provided in the ISO-5349 standard. These guidelines suggest that the magnitude, frequency, direction and duration of vibration exposure are the most important variables for the risk assessment of hand-arm vibration. This current study is focusing on brush cutters owned by one of the biggest caretaker company. These machines have similar brand and age but different in usage time. We inquired seven brush cutters through two years. The manufacturer repaid the vibration level of the new machines, but they doesn't examine, whether extent of the vibration emission is changing for the proper use. We wanted to know that are there any differences between the values of vibration specification after certain time of usage. Is it possible to monitor the alternate movements on graphs according to the vibration sciences? By Ill octave band frequency and FFT analysis we can point at that these brush cutters can show differences in critical frequencies. These biases can influence the hand-arm system and its vibration-strain burdening.
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Transformation of tobacco plants with virEl gene derived from Agrobacterium tumefaciens pTiA6 and its effect on crown gall tumor formation
53-56.Views:137The VirEl protein plays a key role in the transport of VirE2 protein from the bacterium to the plant cell during crown gall tumor induction by Agrobacterium. The virEl gene of A. tutnefaciens pTiA6 was cloned into the plant transformation vector pTd33 yielding pTd93virEl that was introduced into A. tuniefaciens EHA101 and used for tobacco transformation. The presence of the foreign DNA in the putative transgenic plants was confirmed by PCR analysis. Nine of the 41 transformed plants formed only small tumors following infection with the wild-type A. vitis octopine strain AB3. This property was inherited into the T1 generation. The expression of virEl gene in TI plants was demonstrated by Northern blot analysis.
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Comparative analysis of the production risk of major fruit species
119-122.Views:136Hungary possesses excellent agri-ecological potentials even in an international comparison. Despite their decreasing economic weight, agriculture and food production are of great significance, since the supply of the population with high quality and inexpensive food is a fundamental social demand and one of the essentials of the quality of life. The development of vegetable and food production is of great importance both from the aspect of healthy nutrition and comparative advantages (cheap workforce, professional expertise, traditions of production, climate favourable for quality). Within the Hungarian fruit production apple and sour cherry are essential branches in virtue of their significance. It is widely known, that – though to a different extent in the case of certain fruit varieties – satisfaction of the need for appropriate habitat is one of the important conditions of a decent quality and secure production. In my research, I analyse the territorial distribution, production standard and production risk of the Hungarian apple and sour cherry branches. I did not have the opportunity – because of the imperfections of the database – to compare long time series, therefore I examine the random effects affecting production by using the data of 3 years (they have the same content). I ranked and assessed each habitat and the production risks of certain fruit species compared to each other on the basis of indexes of relative deviation.
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Evaluation of the quality of Sorbus fruits belonging to different species
37-41.Views:156The interest in wild growing fruits was increased considerably by the pharmaceutical industry, the cosmetics as well as by the food industry. (Stefanovits-Bányai et al., 2004). Elderberry (Sambucus nigra L), sand thorn (Hippophae rhamnoides L.), cornet (Cornus mas L.), dog rose (Rosa canina L. bearing hips) and the Sorbus species (Sorhus ssp.) have been well known medicinal and/or ornamental plants since long. Recently, precious substances have been detected in their fruits, which are indispensable in healthy foods. Several species and micro-species of the genus Sorbus are components of the native flora of Hungary, and the fruit of some of them have been consumed traditionally, however, they are preferably considered as ornamentals. The nursery of Alsótekeres (Balatonvilágos) maintained some 16 clones of Sorbus species, which are mainly apomictic "micro species" of. the collection. In 2003, a comprehensive analysis of sorb fruits born on apomictic micro species was initiated in order to find those, which will be suitable to establish plantations. It turned out that considerable differences exist between the fruits of individual taxa, however, it is largely influenced by seasonal effects. According to physical measurements, a scale of mean fruit masses could be established. As for chemical ingredients of the fruits, those are of special interest, which are involved mainly in anti-oxidant activities of the organisms (calcium, potash, phosphorus, copper, magnesium).
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Effect of foliar fertilization on leaf mineral composition, sugar and organic acid contents of sweet cherry
45-48.Views:207Influence of a three-year-long foliar fertilization on mineral composition of leaf, content of sugars (glucose, fructose, galactose and sucrose) and organic acids (citric, malic and fumaric acid) of sweet cherry (Prunus avium L.) fruits was studied. The experiment was conducted during 2005-2007 in West Hungary on mature cv. `Germersdorfi 3' grafted on Prunus mahaleb rootstock, planted in 1999. Trees spaced 7 x 5 m, and growing in a calcareous chernozem soil. Trees were foliar-fertilized with potassium (K) as KNO3 and calcium (Ca) as Ca(NO3)2. Potassium spraying was carried out 3 (K1) and 5 (K2) while calcium was applied at 3 (Ca 1), 5 (Ca2) and 6 (Ca3) weeks after full bloom. Beside fruit analysis, complete soil and leaf analysis were done to study the rate of nutrient uptake and its effects on fruit quality. Contents of nutrients of soil and leaf were determined by atomic absorption and spectrophotometric method, while sugars and organic acids in fruit were determined by HPLC. The applied treatments (except K1) had been increasing leaf K significantly compared to the control till ripening. Most of treatments had no significant effect on Ca content of leaf till ripening. From applied treatments only the boron treatments had significant increasing effect on contents of all examined sugars, compared the control. Furthermore, the effect of calcium spraying on the contents of organic acids was significant.
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Illumination-Independent Reflectance Information Acquirement for Leaf Water Potential Measurement on the Example of Satsuma Mandarin
75-79.Views:116Mandarin fruit sugar content can be increased when subjecting the satsuma mandarin tree (Citrus unshiu MARC. var. satsuma) to moderate water stress by mulching during the period of active sugar accumulation, thereby fruit quality improvement can be realized. In the frame of the precision agriculture production system, a non-destructive measuring method development became necessary based on remote sensing, field spectroscopy and image analysis, to be able to measure the degree of water stress. Large amount of visual information have been recorded at ground level, in near infrared, red and green channels by a false color digital camcorder designed specially for remote sensing applications. A method have been found to be able to calculate the absolute reflection of mandarin leaf surface by comparing leaf reflectance with known reference target reflectance, thereby established the basis of further studies in this topic. Leaf absolute reflection can be measured reliably, under variable natural illumination at field conditions. Functional correlation can be searched between visual information and leaf water potential measured by PMS pressure chamber.
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Optimization of sample preparation for determination of antioxidant parameters from one grape berry
93-97.Views:184Berry quality is an important issue in wineprocessing, however evaluating characteristics in a single berry level is very complex. The Phenolic compound analysis is more challenging because of the limited amount of material and a mixture of skin, pulp and seed. No clear protocol exists for evaluating these compounds from single berries. The aim of our investigation was to develop such a protocol. Single whole grape berries samples were homogenized in 20 replicates by 5 different methods. The most effective method was when berry was placed in a mortar and was crushed with a pestle under liquid nitrogen until seeds were clearly visible. Seeds were then taken from the mortar and crushed between two stainless steel plates with a hammer, and returned to the mortar without residues. Homogenization continued until the sample had a powdery appearance. The homogenized samples were taken for further analysis such as total polyphenols, total anthocyanin contents and total antioxidant capacity. Our results demonstrate that single berries could be processed and that the homogenates were suitable for taking chemically uniform subsamples.
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Relationship beetwen the phenological features of pear cultivars and the main meteorological parameters in a gene bank with 555 pear
59-63.Views:171The trees observed are grown at Ujfehert6, Eastern Hungary in a gene bank with 555 pear cultivars. Each of the cultivars was monitored for its dates of: the beginning of bloom, main bloom and the end of bloom and ripe phenophasis separately between I 984 and 2002. We analyzed the statistical features, frequency, distribution of these phenophasis and its' correlation the meteorological variables bet ween the interval. During this period the meteorological database recorded the following variables: daily mean temperature (°C), daily maximum temperature (0C), daily mini m um temperature (0C), daily precipitation (mm), daily hours of bright sunshine, daily means or the differences between the day-time and night-time temperatures (0C). For the analysis of data the cultivars have been grouped according to dates of maturity, blooming period as well as types of the seasons. Groups of maturity dates: summer ripe, autumnal ripening, winter ripe cultivars. Groups of blooming dates: early blooming, intermediate blooming, late blooming cultivars. At all the separated groups we analyzed the relationship between phenophasis and meteorological variables. During the 18 years of observation , the early blooming cultivars started blooming on 10-21 April, those of intermediate bloom date started flowering bet ween 20 April and 3 May, whereas the late blooming group started on 2- 10 May. Among the meteorological variables of the former autumn and winter periods, the winter maxima were the most active factor influencing the start dates of bloom in the subsequent spring. For the research of fruit growing-weather relationships we used simple, well known statistical methods, correlation and regression analysis. We used the SPSS 1 1.0 software for the linear regression fitting and for calculation of dispersions as well. The 1ables made by Excel programme.
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Epiphytic microbiota of apple in integrated and organic growing
85-89.Views:168The surface microbial contamination is of great interest in case of fruits, since they are they potential sources of spoilage or foodborne diseases. The aim of this work was to compare the epiphytic microbiota of food safety concern of different apple cultivars as a function of cultivation methods (organic versus integrated), production year and place. Investigating 47 samples it was found that the average microbial contamination of the apple surfaces was within a certain range,, practically independent of cultivar, growing area and year. The frequency distribution of the surface bacterial, mould and yeast counts did not show significant differences between growing technologies or varieties. The principal component analysis the samples ranked the apple samples into seven groups on the basis of their microbial contamination level, and the discriminant analysis proved the goodness of grouping. The grouping was independent from cultivars and growing methods. No foodborne pathogen Salmonella spp. and Listeria spp. were found on the surfaces of apples.
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Evaluation of supercritical plant extracts on volatile and non volatile biologically active lipophil components
78-83.Views:133Authors dealt more than ten years with the analysis of supercritical extracts. For extraction (SFE) carbon dioxide was used as supercritical solvent. Fractionation of extracts was carried out by releasing the separations pressure at two stages. The extracts were collected as separate samples successively in time.
The traditional extractions were carried out with steam distillation or by using n-hexane and ethanol in Soxhlet apparatus. For the analysis of volatile compounds GC, GC-MS; of non volatile compounds TLC-densitometry and spectroscopic methods were used.
The following general characteristics were established comparing the composition of steam distillated oils with that of volatile SFE fractions. The SFE fractions were richer in monoterpene-esters and poorer in alcohols than the essential oils prepared by traditional way (clary sage, lavandel). Regarding the distributi,n of the monoterpene and sesquiterpene compounds, the SFE fractions contained sesquiterpene hydrocarbon in higher percentage than the distillated oils (e.g. 13-caryophyllene in Salvia fruticosa, (3-caryophyllene, ymuurolene, y-cadinene in Ochnum basilicum). Further the proportion of sesquiterpenes increased in SFE fractions collected successively in time.Significant difference was remarkable in respect of the optical rotationability of lovage oil and SFE fraction which was probably caused by the different ratio between the two ligustilid enantiomers. It was verified in some cases that a part of mono- and sesquiterpenes were present originally in a bounded form (glycosides) in plants. Therefore they appeared in essential oil fractions only after previous acidic treatment (Thymus, Origanum species). During the supercritical extraction the azulenogene sesquiterpene lactones did not transform to azulenes (in chamomile, yarrow), but the non volatile SFE fractions of some Asteraceae plant contained sesquiterpene--lacton of unchanged structure in high quantity (e.g. cnicin in blessed thistle, parthenolide in feverfew). Authors obtained also SFE fractions which were rich in triterpenoids and phytosterols (marigold, common dandelion).
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Farm economic analyis of walnut production
71-74.Views:179In this present study the returning issues and profit conditions of domestic walnut production are investigated. Our objective is to determine the fact that under what conditions our walnut production may be competitive and maintained in an economic way regarding the present economic and market situations. Our analysis was carried out by a deterministic model based on a farm-level data gathering in production enterprises. The total investment cost of an up-to-date walnut orchard is up to 3000 thousands HUF per hectare and turning to
productivity is expected within 8-10th years. These orchards are able to produce a yield of 3 tons per hectare in the years of productivity in a normal year, thus in case of a medium-good selling prices a profit of 500 thousand HUF per hectare may be realized. As a result at the end of the whole lifetime of the orchard (30th year) an internal rate of return (IRR) of 10 to 12% may be calculated and the return is expected in the 20th to 22nd year, which may be considered as a very late return. Taking the 20 to 25% probability of harmful whether phenomena (frost, drought) into consideration, it may be concluded that in good years a performance reaching a yield of 4 tons per hectare is necessary in the long-run in order to achieve the profitable and sustainable production in an economic way. This is managed to reach in only the most up-todate orchards. -
Genotyping Hungarian apricot cultivars for self-(in)compatibility by isoelectric focusing and PCR analysis
69-72.Views:146Self-incompatibility (SI) in flowering plants is a widespread genetic system that promotes out-crossing. In Prunus species the SI is a gametophytic trait, which is controlled by a single multiallelic locus, termed S-locus. S-alleles codify stylar glycoproteins with ribonuclease activity (S-RNases). Our objective was to assess the S-genotype of some Hungarian apricot varieties by isoelectric focusing of stylar RNases as well as by PCR analysis using cherry consensus primers. Consensus primers amplified one or two bands of various sizes. Primers amplifying the 1st intron gained fragments the size of which ranged from 250 to 500 bp; while those amplifying the 2nd intron resulted in fragments of 800-2000 by length. Our data demonstrated that the first intron of the apricot S-RNase gene is shorter than the second one, which coincides with the structure of cherry S-RNase alleles. `Hargrand' (S1S2) and `Harcoe (S1S4) possessed one common S-RNase isoenzyme. Hungarian 'Orias' apricot cultivars showed different bands compared to the previous cultivars, but they shared completely identical patterns confirming that they possess the same S-genotype. 'Bergeron', `Harmat' and 'Korai zamatos' are characterised by an evidently distinct S-RNase pattern. The self-compatible cultivar (`Bergeron') had one allele, which suggests its correspondence to the Sc. Primers for the 2nd intron was unsuccessful in gaining fragments, which indicates that the 2nd intron in the Sc allele is too long to get any amplification. On the basis of our data, identities and differences were revealed in the S-allele constitution of some economically important Hungarian apricot cultivars at protein and DNA levels.
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Genetic transformation of bean callus via Agrobacterium- mediated DNA transfer
49-53.Views:120Callus cultures were induced from hypocotyl of young bean seedlings. Callus developed and maintenaned on B5 medium supplemented with 2mg/1 2,4-D and 1 mg/1 kinetin. The results demonstrate that A. tumefacins-mediated transformation is a convenient method to obtain transient gene expression in callus of bean. The results have shown that the bean callus co-cultivated with A. tumefaciens can be transformed to get heibicide Finale (glufosinate-ammonium) resistant GUS positive tissues. Southern blot analysis of transformed calli showed integration of gusA marker gene carried by a binary vector. Transformed calli were selected on herbicide containing media. Data of molecular analysis (Southern blotting) confirmed the insertion of gusA gene in the genome of herbicide resistant calli with bar gene. There are three evidences that calli are stable transformants: (1) herbicide resistance, (2) GUS activity which is indicative since the coding region containing an intron, (3) the results of Southern hybridization technique.
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Comparative analysis of sweet cherry cultivars on their ecological and biological indicators
14-33.Views:140Sweet cherries are slightly more demanding than sour cherries. It is grown in warmer areas around the world. The relative ecological values obtained for the varieties obtained by extensive data collection differ slightly from the leading descriptions. Warm and demanding. The woody parts tolerate the cool of the winter quite well, the flower buds are damaged by the spring frosts. Its water demand is medium, in the case of 550 mm of annual rainfall, it adorns well on loose soils with good nutrient supply. Airy ground, neutral soil (pH 5.5-7.5) is optimal, but not suitable for areas with strongly calcareous, stagnant, stagnant groundwater. From the start of ripening, sudden rainfall, stormy winds and birds can cause great damage. Highlighting the world’s leading varieties in the study (Bing, Rainier, Chelan, Van and Burlat) (Iezzoni et al., 1991, Faust & Surányi, 1997) - according to relative ecological and biological values, the most popular cherries are mainly they differed from the other varieties based on TB and KB. Open pollination and with it, the productivity of the varieties exceeded the overall variety average precisely because of the breeding objectives. Certainly, the analysis of historical varieties, the oldest landscape and local varieties based on relative ecological and biological values can help further pomological-ecological research.
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Determination of chlorophyll content in case of peach leaf curl disease (Taphrina deformans) with spectral analysis
49-52.Views:288One of the most important conditions of high quality fruit production is the early detection of hytopathologycal infections. The most dangerous disease of peach (Prunus persica) is the Taphrina deformans, which causes serious damages mainly in the years where the weather condition is cool and wet. In this study, healthy and naturally diseased leaves by peach leaf curl were investigated in the laboratory of University of Debrecen, CAAES, Institute of Water and Environmental Management. Both of hyperspectral measurement and the values of spectrophotometer shown the chlorophyll content of diseased leaves were lower than the healthy samples. Based on the experiments the water status of investigated leaves established the infected leaves contained more water in their tissues.
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RAPD analysis of grapevine hybrids and cultivars
63-66.Views:141Utilization of the Randomly Amplified Polymorphic DNA (RAPD) technique as a molecular marker was tested to investigate the relationships between some representative grapevine cultivars and hybrids established at the Department of Genetics and Plant Breeding (CUB), to distinguish clones as well as to characterize various hybrids between species or cultivars and their parents. Vitis vinifera cultivars were easily and successfully distinguished by the RAPD technique and they were grouped according to the traditional taxonomic classification. RAPD patterns of the examined Pinot gris clones proved to be completely identical. Number of generations was reflected by the value of genetic distance of the examined hybrids. Genetic identity of parents and their offsprings was influenced by the selection applied in the process of plant breeding. Parental phenotypic and morphologic characteristics showed high degree of segregation in hybrids, but RAPD analysis revealed that their genetic similarity is considerable. The three Vitis anntrensis clones were properly discriminated from every cultivar and hybrid of Vitis vinifera, i.e. hybrids are much closer to the cultivated grapevine than to V. anzurensis due to the phenotypic selection carried out during the life-cycle of one or two generations.
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Resistance gene Sw-5 of tomato confers resistance to TCSV in Solanum melongena
41-47.Views:137Eggplants transformed with Sw-5 gene, regenerated by organogenesis and somatic embryogenesis, were resistant to the Tomato chlorotic spot virus, while wild plants did present systemic infection. TO plants were selfed and the segregation analysis of T1 and T2 generation indicated the existence of one or more insertion sites. Southern blot analysis confirmed one or two independent insertions in T2 plants. Different lesions associated with the insertion number were observed in TI and T2 plants. T2 plants with two copies displayed faster hypersensitive reactions and characteristic necrotic lesions that contrasted with slower responses and necrotic ring lesions in plants with one copy. These results suggest that the Sw-5 confers resistance to tospovirus in transgenic eggplants and that the resistant phenotype depends on the number of transgene copies.
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Phenological and fl ower morphological studies on different plum and prune cultivars
43-52.Views:22320–22 and 5–7 years of kind collection of 100 cultivars studied phenology and fl oral morphological basis of characteristics in 1994–1998 and 2009–2011. The varieties were collected 3–5 trees in the gene garden, all were on C. 359 myrobalan seedling. Detecting differences between the test cultivars are suitable for stamps, and also show that relatively short-term observations are useful for describing the comparison, to distinguish varieties. The taxonomic ordination of cultivars and groups of fruits suitable to distinguish colors shown that the fl owering and ripening time, the nature of the reproductive organs and even Sharka-rate sensitivity is associated with those groups. Differences between the age of trees mainly sexual organs and fertility were shown. Affecting rate of climatic effects was not as large as the first line of erratic rainfall, air temperature spikes or possible values would be expected. The results of the breed, a gene bank of perception, in particular, the localization of hybrids, clone-type variants are useful in the analysis. Among other things – this is due to late-maturing, relatively well-stored temporarily, and not-blue plums, Sharka-infected tolerant or resistant varieties selection (cf. SURÁNYI 2013a-c) and will be disseminate in Hungary.