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Evaluation of supercritical plant extracts on volatile and non volatile biologically active lipophil components
Published June 6, 2001

Authors dealt more than ten years with the analysis of supercritical extracts. For extraction (SFE) carbon dioxide was used as supercritical solvent. Fractionation of extracts was carried out by releasing the separations pressure at two stages. The extracts were collected as separate samples successively in time.

The traditional extract...ions were carried out with steam distillation or by using n-hexane and ethanol in Soxhlet apparatus. For the analysis of volatile compounds GC, GC-MS; of non volatile compounds TLC-densitometry and spectroscopic methods were used.

The following general characteristics were established comparing the composition of steam distillated oils with that of volatile SFE fractions. The SFE fractions were richer in monoterpene-esters and poorer in alcohols than the essential oils prepared by traditional way (clary sage, lavandel). Regarding the distributi,n of the monoterpene and sesquiterpene compounds, the SFE fractions contained sesquiterpene hydrocarbon in higher percentage than the distillated oils (e.g. 13-caryophyllene in Salvia fruticosa, (3-caryophyllene, y­muurolene, y-cadinene in Ochnum basilicum). Further the proportion of sesquiterpenes increased in SFE fractions collected successively in time.Significant difference was remarkable in respect of the optical rotationability of lovage oil and SFE fraction which was probably caused by the different ratio between the two ligustilid enantiomers. It was verified in some cases that a part of mono- and sesquiterpenes were present originally in a bounded form (glycosides) in plants. Therefore they appeared in essential oil fractions only after previous acidic treatment (Thymus, Origanum species). During the supercritical extraction the azulenogene sesquiterpene lactones did not transform to azulenes (in chamomile, yarrow), but the non volatile SFE fractions of some Asteraceae plant contained sesquiterpene--lacton of unchanged structure in high quantity (e.g. cnicin in blessed thistle, parthenolide in feverfew). Authors obtained also SFE fractions which were rich in triterpenoids and phytosterols (marigold, common dandelion).


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Antioxidant activity of medicinal plants used in phytotherapy
Published June 6, 2001

Oxygen free radicals play an important role in the development of different disorders like inflammatory-immune injury, carcinogenesis, hepatic toxicity and artherosclerosis. The antioxydant role of a wide spectrum of natural products has been established. Flavonoids and other phenolic compounds (proanthocyanidins, rosmarinic acid, hydroxicinnam...ic derivatives, catechines, etc.) of plant origin have been reported as scavengers and inhibitors of lipid peroxidation.

We have studied the antioxidant activity as well as content and composition of natural phenolics in a series of medicinal plants with phytotherapeutical significance. Thus we determined the total phenol contents and studied the composition of flavonoids, polyphenols, phenolic acids of different vegetative and reproductive organs of medicinal plants: Anthriscus cerefolium (L.) Hoffm., Petroselinum crispum L., Cichorium intybus L., Helichrysum arenarium D.C.„cempervivum tectorum L., Taravacum officinale Web.

Characteristic constituents in the various crude drugs were determined by chromatographic (TLC, HPLC) and spectroscopic (UV, UV-VIS) methods. The non specific scavenger activities of the medicinal plant extracts were studied by the chemiluminometric technique. The changes of chemiluminescence intensity of the H,G,•0H-luminol system at increasing concentrations of the H702/ -OH were measured. Inhibitory effects of selected standardized fractions from plants were tested on ascorbic acid induced lipid peroxidation in rat liver and homogenates.

The best correlation were established with total phenolics in some medicinal plants (S. tectorum, T. officinale) while activities in other cases seem to be influenced by flavonoids (P. crispum, H. arenarium, A. cerefolium) and by hydroxicinnamic derivatives (C. intybus).


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