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• Mutation induction in sweet basil (Ocimum basilicum L.) by fast neutron irradiation

  30-38.

  E. Enkhbileg

  A. Fenyvesi

  B. Bíró

  M. G. Fári

  E. Kurucz

  Views:

  313

  Basil species are highly sensitive to exterior environmental conditions and its consequences lead to great economic and agronomic losses. In this research, a mutation method was optimized out for creating a new variety of Ocimum basilicum L., which could tolerate the extreme/extraordinary climatic circumstances or biotic stresses, such as fungal diseases. Fast neutron irradiation was performed on the Hungarian commercial variety seeds with doses of 5 to 60 Gray and grown into fully developed plants. Numerous phenotypical changes like deformed congestion, leaf mutation, and low growth occurred, especially at higher dosages. Then to confirm whether the plantlets had mutation or not, and to detect the molecular variation and relationship, fingerprinting profiles of the developed mutant regenerants and donor plant have been assessed using ISSR markers. 115 loci were yielded, ranging from 0.2 to 1.5 kb, out of which 110 loci were polymorphic in nature, representing 95.6% polymorphism. The most suitable primer to determine the genetic diversity within the Ocimum species was the UBC-856 with 0.42 PIC and 4.1 MI values.

• Nutrition of the micropropagated fruit trees in vitro and ex vitro

  43-46.

  I. Balla

  J. Vértesy

  Gy. Végváry

  E. Szűcs

  T. Kállay

  I. Vörös

  B. Bíró

  Views:

  141

  Some experience or details are introduced in connection with the nutrient uptake of micropropagated fruit trees in the different phase of the in vitro or ex vitro development. It can be stated, that the plants during the micropropagation procedure are overfed. Special careful nutrient supply is necessary during the acclimatization.

• Determination of chlorophyll content in case of peach leaf curl disease (Taphrina deformans) with spectral analysis

  49-52.

  P. Riczu

  Gy. Bíró

  E. Sulyok

  A. Nagy

  J. Tamás

  Z. Szabó

  Views:

  288

  One of the most important conditions of high quality fruit production is the early detection of hytopathologycal infections. The most dangerous disease of peach (Prunus persica) is the Taphrina deformans, which causes serious damages mainly in the years where the weather condition is cool and wet. In this study, healthy and naturally diseased leaves by peach leaf curl were investigated in the laboratory of University of Debrecen, CAAES, Institute of Water and Environmental Management. Both of hyperspectral measurement and the values of spectrophotometer shown the chlorophyll content of diseased leaves were lower than the healthy samples. Based on the experiments the water status of investigated leaves established the infected leaves contained more water in their tissues.