Vol 25 No 1-2 (2019)
Cikkek

Mutation induction in sweet basil (Ocimum basilicum L.) by fast neutron irradiation

Published July 29, 2019
E. Enkhbileg
University of Debrecen, Faculty of Agricultural and Food Sciences and Environmental Management, Department of Botany, Biotechnology and Plant Physiology 4032 Debrecen, Böszörményi út 138, Hungary
A. Fenyvesi
Hungarian Academy of Sciences, Institute for Nuclear Research, Cyclotron application laboratory 4026 Debrecen, Bem tér 18/c
B. Bíró
Hungarian Academy of Sciences, Institute for Nuclear Research, Cyclotron application laboratory 4026 Debrecen, Bem tér 18/c
M. G. Fári
University of Debrecen, Faculty of Agricultural and Food Sciences and Environmental Management, Department of Botany, Biotechnology and Plant Physiology 4032 Debrecen, Böszörményi út 138, Hungary
E. Kurucz
University of Debrecen, Faculty of Agricultural and Food Sciences and Environmental Management, Department of Botany, Biotechnology and Plant Physiology 4032 Debrecen, Böszörményi út 138, Hungary
View:
pdf
How to Cite

APA

Enkhbileg, E., Fenyvesi, A., Bíró, B., Fári, M. G., & Kurucz, E. (2019). Mutation induction in sweet basil (Ocimum basilicum L.) by fast neutron irradiation. International Journal of Horticultural Science, 25(1-2), 30-38. https://doi.org/10.31421/IJHS/25/1-2./2912

Abstract

Basil species are highly sensitive to exterior environmental conditions and its consequences lead to great economic and agronomic losses. In this research, a mutation method was optimized out for creating a new variety of Ocimum basilicum L., which could tolerate the extreme/extraordinary climatic circumstances or biotic stresses, such as fungal diseases. Fast neutron irradiation was performed on the Hungarian commercial variety seeds with doses of 5 to 60 Gray and grown into fully developed plants. Numerous phenotypical changes like deformed congestion, leaf mutation, and low growth occurred, especially at higher dosages. Then to confirm whether the plantlets had mutation or not, and to detect the molecular variation and relationship, fingerprinting profiles of the developed mutant regenerants and donor plant have been assessed using ISSR markers. 115 loci were yielded, ranging from 0.2 to 1.5 kb, out of which 110 loci were polymorphic in nature, representing 95.6% polymorphism. The most suitable primer to determine the genetic diversity within the Ocimum species was the UBC-856 with 0.42 PIC and 4.1 MI values.

Downloads

Download data is not yet available.