Vol 12 No 4 (2006)
Cikkek

Resistance gene Sw-5 of tomato confers resistance to TCSV in Solanum melongena

Published September 26, 2006
E. A. T. Picoli
Departamento de Biologia Vegetal, Universidade Federal de Vicosa, 36570-000 Vicosa, MG, Brazil; Nucleo de Biotecnologia Aplicada a Agropecuaria (BIOAGRO), Universidade Federal de Vicosa, 36570-000 Vicosa, MG, Brazil
G. S. A. Lima
Departamento de Fitopalotogia, Universidade Federal de Vicosa, 36570-000 Vicosa, MG, Brazil; Empresa Pernambucana de Pesquisa Agropecuaria, 50761-000, Recife, PE, Brazil
D. Lau
Departamento de Fitopalotogia, Universidade Federal de Vicosa, 36570-000 Vicosa, MG, Brazil; Nucleo de Biotecnologia Aplicada a Agropecuaria (BIOAGRO), Universidade Federal de Vicosa, 36570-000 Vicosa, MG, Brazil
J. F. C. Oliveira
Universidade Estadual Paulista Julio de Mesquita Filho, Departamento de Tecnologia, 14884900 Jaboticabal, Sp, Brazil
M. L. Laia
Universidade Estadual Paulista Julio de Mesquita Filho, Departamento de Tecnologia, 14884900 Jaboticabal, Sp, Brazil
F. M. Zerbini
Departamento de Fitopalotogia, Universidade Federal de Vicosa, 36570-000 Vicosa, MG, Brazil; Nucleo de Biotecnologia Aplicada a Agropecuaria (BIOAGRO), Universidade Federal de Vicosa, 36570-000 Vicosa, MG, Brazil
S. H. Brommonschenkel
Departamento de Fitopalotogia, Universidade Federal de Vicosa, 36570-000 Vicosa, MG, Brazil; Nucleo de Biotecnologia Aplicada a Agropecuaria (BIOAGRO), Universidade Federal de Vicosa, 36570-000 Vicosa, MG, Brazil
M. G. Fári
University of Debrecen, Center of Agricultural Sciences, H-4032 Debrecen, Böszörményi út 138, Hungary
W. C. Otoni
Departamento de Biologia Vegetal, Universidade Federal de Vicosa, 36570-000 Vicosa, MG, Brazil; Nucleo de Biotecnologia Aplicada a Agropecuaria (BIOAGRO), Universidade Federal de Vicosa, 36570-000 Vicosa, MG, Brazil
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How to Cite

APA

Picoli, E. A. T., Lima, G. S. A., Lau, D., Oliveira, J. F. C., Laia, M. L., Zerbini, F. M., Brommonschenkel, S. H., Fári, M. G., & Otoni, W. C. (2006). Resistance gene Sw-5 of tomato confers resistance to TCSV in Solanum melongena. International Journal of Horticultural Science, 12(4), 41-47. https://doi.org/10.31421/IJHS/12/4/677

Abstract

Eggplants transformed with Sw-5 gene, regenerated by organogenesis and somatic embryogenesis, were resistant to the Tomato chlorotic spot virus, while wild plants did present systemic infection. TO plants were selfed and the segregation analysis of T1 and T2 generation indicated the existence of one or more insertion sites. Southern blot analysis confirmed one or two independent insertions in T2 plants. Different lesions associated with the insertion number were observed in TI and T2 plants. T2 plants with two copies displayed faster hypersensitive reactions and characteristic necrotic lesions that contrasted with slower responses and necrotic ring lesions in plants with one copy. These results suggest that the Sw-5 confers resistance to tospovirus in transgenic eggplants and that the resistant phenotype depends on the number of transgene copies.

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