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Isolation and identification of endophytic fungi connected to Grapevine Diseases, from the Tokaj wine region, Hungary
61-66Views:224Grapevine Trunk Diseases (GTD) is one of the most important diseases in vineyards worldwide, which can be found in Hungarian vineyards as well. In Hungarian wine regions there is very little information about the occurrence of pathogens which cause GTD, in case of Tokaj wine region there is no knowledge about that, what kind of pathogens can be found in the vineyards.
The objective of our research is to assess the situation and occurrence of GTD in Tokaj wine region in cooperation with local specialists, as well as identification of pathogens which were isolated from the diseased trunks by morphological and genetic basis.
We were able to isolate endophytic fungi from all sampled grape trunk. The majority of them were determined as Diplodia seriata not only based on colony morphology, but also determined by rDNA sequences.
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Testing disease resistance in autumn wheat genotypes by means of field experiments
30-40Views:113According to our scientific results we can state that we have to use integrated pesticides management in crop protection against the diseases of winter wheat. One of the most important elements of IPM is to select a genotype characterised by good resistance to diseases (and by high yield ability and excellent baking quality). It is especially important that the wheat variety have tolerance against not only to one or two leaf and spike (grain) diseases, but „complex” tolerance. It is not necessary to give up the growing of a variety which has susceptibility to different diseases because we can protect it using appropriate chemical management. In the intensive growing stage of wheat (BBCH 32-37) we can use a noncompulsary fungicide-treatment (depending on e. g. the infection, ecological conditions) and, at the beginning of the flowering stage
(BBCH 59-65), we have to use a compulsary fungicide-treatment (in spite of e. g. special weather conditions, resistance genotype)to ensure high yield and good quality. -
Phytoplasma diseases on fruits in Hungary
24-29Views:175In the last twenty years, three phytoplasma diseases were identified in Hungary, viz. European Stone Fruit Yellows (ESFY) (caused by Candidatus Phytoplasma prunorum), pear decline (caused by Candidatus Phytoplasma pyri), and apple proliferation (caused by Candidatus Phytoplasma mali). Candidatus Phytoplasma prunorum was isolated from apricot, peach, plum and japanese plum. Cacopsylla pruni the vector of ESFY was also isolated and identified. Infection of Candidatus Phytoplasma pyri was diagnosed from pear and Candidatus Phytoplasma mali was found on apple and pear. The three phytoplasmas cause different damages on their host plants. The most economically important phytoplasma disease is the ESFY. It seriously impairs apricot and japanase plum trees. After infection of apricots and japanese plums show yellowing and defoliation, and within a few years die in apoplexy-like symptoms. The disease on japanese plum is so severe that this fruit practically can not be cultivated in Hungary. Pear decline is the most serious problem especially in intensive pear plantations. The vector Cacopsylla pyri, C. pyrisuga and C. pyricola can be found in almost all pear orchards. Because of the regular presence of psyllids in intensive pear orchards the insecticide control is necessary. Apple proliferation is not an important disease in Hungary. All of our isolations of ’Candidatus Phytoplasma mali’ occured in organic orchards and record was not available in Hungary lately.
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Dieback of apricot plantations caused by 'Ca. Phytoplasma prunorum' in Borsod-Abaúj-Zemplén county (Northern-Hungary)
34-41Views:132Plant diseases caused by phytoplasmas have increasing importance in all over the world for fruit growers. Lately, phytoplasma diseases occur on many fruit varieties and responsible for serious losses both in quality and quantity of fruit production. In the long-run these diseases cause destruction of fruit trees. The apricot phytoplasma disease (Ca. Phytoplasma prunorum) was first reported in Europe in 1924 from France. In 1992 the disease has also been identified in Hungary. On the base of growers' signals serious damages of "Candidatus Phytoplasma prunorum" Seemüller and Schneider, 2004 (formerly: European stone fruit yellows phytoplasma) could be observed in different stone fruit plantations in the famous apricot-growing area nearby Gönc town, Northern-Hungary. Field examinations have been begun in 2009 in several stone fruit plantations in Borsod-Abaúj-Zemplén County mainly in Gönc region which is one of the most important apricot growing regions in Hungary, named “Gönc Apricot Growing Area”. Our goals were to diagnose the occurrence of Ca. Phytoplasma prunorum on stone fruits (especially on apricot) in the North-Hungarian growing areas by visual diagnostics and confirm data by laboratory PCR-based examinations. All the 28 collected samples were tested in laboratory trials and at 13 samples from apricot, peach, sour cherry and wild plum were confirmed the presence of phytoplasma (ESFY). On the base of observations it seems evident that the notable losses caused by "Ca. Phytoplasma prunorum" is a new plant health problem to manage for fruit growers, especially apricot producers in Hungary.
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Selenium-speciation experiments from soil samples by accept of ionchromatography-inductively coupled plasma mass spectrometry (IC-ICP-MS) method
106-111Views:212In these days, selenium is one of the most investigated microelements. It has an important proven role in many vital processes. Selenium deficiency can play a role in the contraction of many diseases (e.g. cancer, heart diseases, etc.) and in the aggravation of their clinical aspect directly or indirectly. In this paper, we study the soil samples of an outdoor experiment for the conversion of the inorganic selenium salt that was spread out in different doses. The danger of the wash-out effect was also investigated. Our experiments showed, that most of the inorganic selenite transforms to selenate form during the years, and in this selenate form it moves to the deeper layers of soil.
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Viticulture of Hungary
175-182Views:350For several thousand years, vine has been grown in the Carpathian basin, where the climatic and soil conditions are favourable for viticulture. During this long period, vine growing has been developing until it reached its current niveau. We grow propagation material, table grape and wine grape. The produced wine grape varieties are grown on the largest area, of which 72% is white wine and 25% is red wine. Only 3% remains for table grapes. The surface of vineyards is about 63 000 ha, which can be found in 22 wine regions. The biodiversity of vine is very rich in our country. There are a lot of native and valuable bred varieties and clones in cultivation. The resistant and winter frost resistant vine varieties have an important role in our continental climate. The in 75% of the vineyards is on hills and mountains, 25% of them is on the Great Hungarian Plain. High cordon with cane pruning dominates the training and pruning system.
Unfortunately, vine stocks suffer from a lot of diseases and by using pesticides we pollute the nature. Viticulturists in Hungary produce excellent raw material and provide oenologists with them who make wine of excellent quality.
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Describing Fusarium diseases on maize in 2013 using data from several production sites
60-64Views:217As in other parts of the world, the frequency of weather extremes has increased greatly in Hungary in recent years. This means that maize production is faced with greater risks from all aspects: nutrient replacement, irrigation, plant protection. This is especially true of fusarium diseases. In a continental climate, the pathogens causing the most serious problems are species belonging to the Fusarium genus. They infect the ears, which – besides reducing the yield – poses considerable risk to both human and animal health due to the mycotoxins produced by them. Depending on which Fusarium species are dominant at a given location, changes can be expected in the level of infection and in the quality deterioration caused by the mycotoxins they produce. Fusarium spp. not only damages the maize ears but when pathogen attacks the stalk, the plant dies earlier, reducing grain filling and resulting in small, light ears. In addition, the stalks break or lodge, resulting in further yield losses from ears that cannot be harvested. The degree of infection is fundamentally determined by the resistance traits of the maize hybrids, but also a great role in that region Fusarium species composition as well.
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The examination of the agronomy, the amount of yield, and the yield stability of winter wheat varieties
61-72Views:129Our research was carried out at University of Debrecen Centre for Agricultural Sciences Faculty of Agriculture Institution of Plant Sciences Látókép Research Institute through the breeding year of 2003/2004, 2004/2005 and 2005/2006 using cherrnozem soil. In our research we tested 14 chosen autumn wheat varieties during the three crop years.
The different varieties showed very dissimilar ability of resistance against diseases through the three crop years. We could observe both susceptible and resistant varieties. Susceptible varieties got diseases even in favourable crop years. The observed winter wheat varieties showed higher susceptibility against helminthosporium (21.8%) and leaf rost (16.4%). Among the 14 varieties we experienced the least susceptibility in the case of ‘Gaspard’ and ‘GK Kalász’. The research showed that the disease of fusarium undoubtedly depends on the features of the crop year.
In terms of stem solidity we experienced big differences. Among all the observed winter wheat varieties the mid-late ripening ‘Gaspard’ showed the best results in the average of the three years, only 5.3% was beaten down.
The three ripening group of the winter wheat showed the following average yield in the average of three years: 7065 kg/hectare (early ripening varieties), 7261 kg/hectare (late ripening varieties), 6793 kg/hectare (mid-late ripening varieties). Among all the observed varieties the early ripening ‘Flori 2’ produced the biggest yield (7692 kg/hectare).
During the three crop years we reached very different amounts of yield which means that weather conditions had a telling affect on yield. In 2004 we reached an excellent average yield in all the tree breeding groups because of the favourable weather conditions. In 2005 we had a moderate amount of yield because of the unfavourable weather conditions of winter. The year of 2006 showed the smallest amount of yield which is due to the fact that the plant grew less thick than usually.
There were significant differences among the observed varieties in the term of yield, which can be attributed to dissimilar biological basics.
One of he most important questions is the yield stability of the varieties. We had extremely different results at this field. Speaking in general terms we can state that both weather conditions and genetical abilities have a determining effect on yield. In the case of winter wheat varieties the rate of yield fluctuation was quite big, moving in the interval of 33.7-70.3%. Among all the observed varieties ‘Gaspard’ showed the best yield stability (33.3%). -
Health factor in food consumption
125-132Views:88Consumer lifestyle and health are relevant factors to understanding consumption preferences. In the last few decades the number of lifestyle diseases has dramatically increased. The main cause for these diseases is the change in lifestyle; including a lack of attention to physical activity and good nutrition. Health and lifestyle are important factors by purchase decision process. In accordance with these, I examine the consumer behaviour toward soft drinks with special regards to healthy lifestyle and the state of health. My examinations can be considered mainly as a
qualitative research, which can serve as a basis for further analyses and research, however, the conclusions and experience gained from it are worthy of consideration. I differentiated five soft drink categories: ice tea, carbonated soft drinks, fruit juices, mineral waters, sport and energy drinks and studied the consumer behaviour toward them. The study focuses on the consumption of these and the factors influencing their purchase with special regards to lifestyle. -
Study of plant production modells with different intensity in winter wheat production
51-60Views:127The effects of crop rotation, nutrient supply and plant protection technologies were examined on the yield of Mv PÁLMA winter wheat variety and on the most important diseases of ear and leaf. Our experiments were carried out on chernozem soil in the Hajdúság in 2006 and 2007, and three plant protection technologies (extensive, average, intensive) and three irrigation variations (without irrigation, irrigated with 50 mm, irrigated with
100 mm) were applied in different crop rotation systems.
In the triculture crop rotation a higher rate of infection was observed than in the biculture crop rotation, because the vegetative growth was more expressed after pea and these microclimatic factors were favourable for the development of pathogens.
In the triculture crop rotation (pea – wheat – maize) the powdery mildew, DTR and leaf rust of wheat were present in both examined years (powdery mildew 5-15%, DTR 14-42%, leaf rust 8-37% in cropyear 2005/2006, powdery mildew 12-32%, DTR 9-29%, leaf rust 8-26% in cropyear 2006/2007). Fusaria could be observed in 2006 (depending on the plant protection technologies and nutrient supply in the biculture 7-27% and in the
triculture 5-19%). With higher amounts of fertilizers the rate of infection increased and reached its maximum at the highest dose of nutrient supply (N200 +PK).
We observed the highest rate of infection by ear and leaf diseases in the case of the extensive technology, while this rate could be considerably reduced by the application of the intensive technology.
Both in 2006 and 2007, yields were the highest at the N100-200+PK levels in the triculture after pea (6028-7939 kg ha-1 in cropyear 2006, 6578-8690 kg ha-1 in cropyear 2007 depending on plant protection technologies), and at the N150-200+PK levels in the biculture after maize (6096-7653 kg ha-1 in cropyear 2006, 4974-8123 kg ha-1 in cropyear 2007 depending on the plant protection technologies). The highest yield maximums were
reached when pea was the forecrop. The yields on the experimental plots of the intensive plant protection technology was 224-2198 kg ha-1 higher (depending on the forecrop) compared to the plots where the extensive technology was used.
The highest yield without irrigation was at the N150+PK both in biculture and triculture crop rotation. Among the irrigated variations Ö2 and Ö3 at N200+PK fertilisation resulted in the highest yield in the biculture crop rotation, while the N100+PK level in triculture system. In the biculture crop rotation the extra yield was 14-51% higher (575-1225 kg ha-1 depending on plant protection technology) when 50 mm water was irrigated, and
15-54% higher (778-2480 kg ha-1) if 100 mm irrigation was applied comparing to the non-irrigated versions. The yield was 7-17% higher (560-1086 kg ha-1) in the Ö2 irrigation variation, and 8-23% (691-1446 kg ha-1) higher in the Ö3 irrigation variation compared to Ö1 irrigation variation (non-irrigated).
A correlation analysis was made to reveal the connection between the yield, the amount of fertilizers, the rate of infection, the plant protection technologies and the forecrops. Strong positive correlation (0.846) was found between year and fusaria infection. Strong positive correlation was observed between fertilization and powdery mildew infection (0.525), fertilization and DTR (0.528), fertilization and yield quantity (0.683). Lower
correlation was found between fertilization and leaf rust infection (0.409), and forecrop and yield (0.472), recpectively. Negative correlation was calcutated between plant protection technologies and DTR (-0.611), and plant protection technologies and leaf rust (-0.649). -
Effect of Ozone Exposure on Phytopathogenic Microorganisms on Stored Apples
9-13Views:113The aim of our study was to clarify the effect of ozone exposure on several phytopathogenic fungi on stored apple fruits under different storage conditions. The study was conducted at Bistrita, Romania, in the storehouse of an experimental apple orchard in 2002 and 2003. Two widely grown apple cultivars (‘Jonathan’ and ‘Golden Delicious’) were used. General microbial examination of the fruits was made during storage in order to identify the most important storage pathogens. Efficacy of six ozone treatments was evaluted on fruit decay caused by phytopathogenic fungi. Monthly observations (January, February, March and April) were made of the degree of decay and three measurements were assessed (disease frequency, disease intensity and degree of attack). Our results showed that the most important phytopathogenic fungi during storage was blue mold, caused by species of Penicillium. Disease frequency of apple fruits was very high on cv. ‘Jonathan’, much higher than on cv. ‘Golden delicious’. Ozone treatments (25 ppm ozone for 0.5 and 1.5 hours in November) caused significantly lower disease incidence on stored apple than all other ozone treatments. For longer storage, it seems that additional ozone treatments in February increased treatment efficacy. Cv. ‘Golden delicious’ seemed to be more resistant to storage diseases than cv. ‘Jonathan’ both on the untreated and treated fruits. The effect of the ozone treatments was also the most effective when 25 ppm ozone was applied for 0.5 and 1.5 hours in November.
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Changes of relative chlorophyll content at maize smut inoculated hybrids
55-58Views:285The leaf chlorophyll content analysis is important for several reasons. The natural or anthropogenic stressors directly effect on the chlorophyll content. Through the measurement of the chlorophyll content it is possible to obtain data concerning the physiological status of the plant, moreover the chlorophyll content is closely related to the nitrogen content, so it is linked to photosynthesis and the photosynthetic activity which determine biomass production.
One of the most common symptoms of plant diseases is the larger and smaller interveinal chlorotic areas. These might be local, or expand to the whole plant. There are multiply reasons of chlorosis such as reduction of chlorophyll content, unfavorable effects on the chlorophyll content,disorders regarding function of chloroplasts or ultimately destruction of the chloroplasts. Although such a chlorotic deviancy can contribute to significant losses in photosynthesis; however the underperformance photosynthesis of the sick plants is a more complex process.
As we unambiguously experienced during our investigations on common smut that the infected maize plants most common accompanying symptom was chlorosis on the leaves, so it is especially important to examine how the infection influenced on the chlorophyll content of different hybrids. -
Susceptibility of maize hybrids to corn smut diseas
39-42Views:189Corn smut disease is one of the most common maize diseases. In the previous years the disease lost from its importance, however nowadays it is becoming more and more important and widespread. The protection against it encounters great difficulties, therefore it is important to emphasize prevention and resistance based protection. The aim of the research was to investigate under laboratory circumstances the resistance of Hungary’s most commonly cultivated maize hybrids to corn smut disease. Twelve species were investigated. We distinguished two susceptibility groups within the hybrids based on the investigated parameters. The results are intended to assist the protection against corn smut disease and to make a suggestion for the farmers so that they could apply more environmental friendly and economical production.
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The importance of selenium in the living world and in the scientific research
278-286Views:233Presently, selenium (Se) is one of the most investigated microelements. It has an important proven role in many vital processes. Directly or indirectly, selenium deficiency can play a role in the development of many diseases. On the other hand, the concentration range in which selenium is essential is narrow; there is a narrow gap between necessary and toxic content in dietary intake. In this context, selenium contamination poses a further health risk for people if they live near the industrial areas and mining activity.
In this paper, we comprehensively introduce the very important trace element selenium. We studied the base parameters, deposit, analytic and deficiencies, problem of contamination and also the solution of contamination problems of selenium. -
Selection of powdery mildew resistant and susceptible grapevine genotypes with molecular markers
100-104Views:167Incorporation of competitive quality and resistance against the most important fungal diseases (powdery and downy mildew) in a cultivar is one of the most important aims of grapevine breeding. In the 20th century, the most advanced results in grapevine resistance breeding were achieved by French researchers. They used resistant cultivars in more than 30% of their growing areas. In these varieties, North American wild Vitis
species were the resistance gene sources. The discovery of immunity-like resistance of Muscadinia rotundifolia opened new perspectives in resistance breeding. M. rotundifolia harbours a dominant powdery mildew gene, providing resistance in highquality cultivars after back-crosses with V. vinifera varieties. M. rotundifolia has been involved in the Hungarian grape breeding programs since 1996, thanks to a French-Hungarian variety exchange. In addition to traditional selection methods, application of MAS (Marker Assisted Selection) based on various types of
molecular markers, can provide additional tools for these efforts. Run1 locus, responsible for powdery mildew resistance, was identified in Muscadinia rotundifolia. Molecular markers closely linked to this locus are very significant in screening progenies deriving from M. rotundifolia and V. vinifera crosses, making possible the discrimination between resistant and susceptible genotypes at DNA level. In our analyses BC5 progeny of {(M. rotundifola×V. vinifera) BC4}×Cardinal (V. vinifera) tested for powdery symptoms were analysed with PCR-RFLP (GLP1- 12P1P3) and microsatellite markers (VMC4f3.1, VMC8g9). Our results proved the applicability of the linked markers and reliability of marker assisted selection. -
Anti-Oxidants in Agriculture
195-200Views:115Today’s programmes aiming at enhancement of fruit and vegetable consumption have been intensified. In the unanimous view of experts, different health problems, such as those of the immune system, inflammations, and even certain cancerous diseases can be prevented and/or cured with regular consumption of fresh (raw) fruit and vegetables. It is well-known fact that among the biologically valuable components, antioxidant compounds – C- and E vitamins, as well as carotinoides – play an important role. In this field, Hungary can expect success in the future since it has excellent plant genetic stock. Regretfully, national data banks regarding cultivation technology, cultivation areas or varieties for fruits and vegetables and their antioxidant contents do not exist in Hungary. Nevertheless, in connection with the so-called “Hungaricums” its existence would be of urgent necessity. Such excellent Hungarian products are – among others – a lot of sour cherry varietiles, the Szeged green pepper and the Makó onion. They enjoy high priority as “Hungaricums” even in the European Union and such activities that support these kinds of products should be enhanced by intense and consequent research work, which may prove their role as functional foods. Presently’ the USA leads in the research of antioxidant compounds of sour cherry, and so far 17 of these compounds have been found partly in Hungarian varieties. Similar research on green, and ‘pritamin’ peppers have not gone so far since they were limited only for seasoning paprika. In Hungary, studies on onion and garlic have not been performed. It should be mentioned that due to the continental climate, these products may be cultivated, consumed or processed only in a limited period. Taking this into consideration, processing and conserving methods are needed which make the consumption of these fruit and vegetables as functional food possible year-round. Scientific establishment of this set of questions is of current concern, because consumption of these products could have an important role in improving the health status of the Hungarian population in the future.
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Cytochrome b diversity of Hungarian Botrytis cinerea strains
18-21Views:120In the mitochondrion of eukaryotes, cytochrome b is a component of respiratory chain complex III. Cytochrome b is encoded by the
cytochrome b (CYTB) gene located in the mitochondrial genome. The fungicidal activity of QoIs relies on their ability to inhibit mitochondrial respiration by binding at the so-called Qo site (the outer quinol-oxidation site) of the complex III. Since their introduction, QoIs (like azoxystrobin) have become essential components of plant disease control programs because of their wide-ranging efficacy against many agriculturally important fungal diseases like grey mould on various crops. QoI resistance primarily arises from a target-site-based mechanism involving mutations in the mitochondrial CYTB. As the management of grey mould is often dependent on chemicals, the rational design of control programs requires the information about the diversity of genes connected with resistance in field populations of the pathogen.
Monospore B. cinerea field isolates has been collected during 2008-2009 from different hosts in Hungary. PCR fragment length analysis
indicated the high frequency presence of type large intron in the isolates while in a few strains G143A substitution could also be detected.
These results indicated the heterogeneity of CYTB in the Hungarian B. cinerea populations, which possibly involve the heteroplasmy of this
mitochondrial gene, moreover indicates the existence op azoxystrobin resistant populations in Hungary.
This work was supported by NKFP-A2-2006/0017 grant. Erzsébet Fekete is a grantee of the János Bolyai Scholarship (BO/00519/09/8). -
Inheritance of Plant and Ear Height in Maize (Zea Mays L.)
34-38Views:459Plant and ear height are very important characters not only for describing new varieties of maize (Zea mays L.), but for green and dry matter production, and even for grain yield. Significant positive correlations have been reported by various authors between plant height and stover yield, plant height and dry matter yield, and plant height and grain yield. The height of the main ear is also correlated to plant height. It depends on the variety or the environment, but is likely to be the same height within a population. Many environmental and agronomical factors (e.g. plant density, fertilization, pests and diseases) influence the expression of these characters, which are not quality traits. Their expression is controlled by many genes and by the interactions between these genes. The heritability of these traits is high and they show significant genotypic variability and positive heterosis, as reported in many research publications.
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The effect of water-stress on the mineral nutrition of fruit plantations
187-192Views:136Besides agro-techniques the climatic conditions play an important role in agricultural production. Weather extremes are
significant hazards to many horticultural regions all over the word. It has a profound influence on the growth, development and yields of a
crop, incidence of pests and diseases, water needs and fertilizer requirements in terms of differences in nutrient mobilization due to water
stresses. Nowadays, the weather extremes cause more and more problems and significant hazards to many horticultural regions in Hungary.
The aim of this study is to explore the problems of nutrient uptake followed from climatic anomalies and response it. In this study
we focus on water supply problems (water-stress).
Reviewing the effects and nutrient disorders caused by climatic anomalies, the following statements can be taken:
· Nutrient demand of trees can be supplied only under even worse conditions.
· The most effective weapon against damage of climatic anomalies is preventative action.
· Proper choice of cultivars, species and cultivation should provide further possibilities to avoid and moderate the effects of
climatic anomalies.
· Fruit growing technologies especially nutrition should be corrected and adjusted to the climatic events as modifier factors.
· The role of foliar spraying, mulching and fertigation/irrigation is increasing continuously.
· Urgent task of the near future is to correct and adjust the tested technologies of fruit growing according to these climatic events as
modifier factors.
Optimal nutrient supply of trees decreases the sensitivity for unexpected climatic events. To solve these problems supplementary, foliar
fertilization is recommended, which adjusted to phonological phases of trees. Moreover, mulching is regarded as an excellent water saving
method. -
The bioactive compounds of sour cherry (Prunus cerasus) with special regards to antioxidant activity and antioxidant density
83-87Views:210Evidence suggests that a diet high in fruits and vegetables may reduce the risk of chronic diseases, such as cardiovascular disease and cancer, and phytochemicals including phenolics, flavonoids and carotenoids from fruits and vegetables may play a key role in reducing chronic disease risk. Recent research has proven that sour cherry (Prunus cerasus L.) is a valuable natural source of some bioactive compounds important in human health preservation.
In our work, we identified the total antioxidant capacity and ”antioxidant density” of sour cherry varieties named ”Újfehértói fürtös”, ”Debreceni bõtermõ”, ”Kántorjánosi” and ”Érdi bõtermõ” and those of the ”Bosnyák” sour cherry clones. ”Antioxidant density” is a biological value indicator obtained in a synthetic way, which indicates the antioxidant capacity of the particular food, e. g. fruit and vegetable, per 1 Calorie. -
The impact of applying foliar fertilizers on the health condition of maize
105-109Views:194The nutrient supply of plants is becoming more important in plant protection since the appropriate condition of plants can be ensured by optimal and satisfactory nutrient supply to avoid nutritional deficiency diseases. Due to the extreme weather conditions, plants are forced to face several stress factors, which leads to deterioration of the plant health. The increasing occurrence of droughts poses threat to nutrient uptake through the roots since all the nutrients can be accessible to the plants only if they are in dissolved form in the soil – which is not possible in the absence of water. Therefore, the importance of foliar fertilizer is becoming a more and more significant part of the nutrient supply, because with the help of this technology the development of any nutrient deficiency can be prevented.
In this experiment, we focused on the efficiency of two different foliar fertilizers on maize. Foliar fertilizers were applied two times, once in the stage with 8 leaves and tasseling phenophase of the maize. To verify the efficiency of the foliar fertilizers, the chlorophyll content of untreated and treated plant’s leaves was measured after each application. Moreover, the length and diameter of maize cobs, thousand kernel weight, protein, oil and starch content were also measured, and the results were compared to the untreated (control) ones. According to the results, in all aspects significant differences were observed and due to the laboratory analysis of leaves, in the case of magnesium and zinc supply the foliar fertilizers were able to prevent the development of nutrient deficiency.
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Q-PCR analysis of the resistance of Hungarian Botrytis cinerea isolates toward azoxystrobin
41-44Views:120The genes being in the mitochondrial DNA primarily encode the enzymes of cellular respiration. Fungicides belonging to the family of quinol oxidase inhibitors (QoIs) play on important role in the protection against several plant diseases caused by fungi. These fungicides bind to the cytochrome bc1 complex so they block electron transport between cytochrome b and cytochrome c1. This way these fungicides inhibit the ATP synthesis consequently they inhibit the mitochondrial respiration. The QoI resistance has two mechanisms. One of them is the point mutation of the cytochrome b gene (CYTB), e.g. the substitution of a single glycine by alanine at position 143 results in high-resistance. The other is the cyanide-resistant alternative respiration sustained by the alternative oxidase.
In a cell there are several mitochondria. The phenomenon when the genomes of all mitochondria in the cell are identical is called homoplazmy. If in the cell there is wild and mutant mitochondrial DNA this is called heteroplasmy. Whether the mutation in the mitochondria causes fenotypical diversity or does not depend on the dose, i.e. it depends on the percentage of the changed mitochondrials. During our work we investigated Botrytis cinerea single spore isolates which have been collected in 2008-2009 on different host plants. Our goal was to decide whether heteroplasmy influences the level of resistance. We managed to detect the change of the level of heteroplasmy, so the change the level of the resistance due to the treatment with fungicide. -
The effect of different fat content fish feed on the production parameters and meat quality of the hybrid striped bass
33-37Views:203The interspecific ’hybrid striped bass’ is the result of cross breeding. Their offspring have got good chances of growth and tolerate extreme water temperatures and the content of dissolute oxygen more than their parents. Furthermore, they are more resistant to diseases. Therefore, the hybrid is perfectly suitable for intensive fishery production.
Our experiment lasted for seven weeks. We analysed the indices of growth and production of the hybrid as the consequence of nurturing them with two feeds of high nutritive value during this period. We examined the content of fat and of fatty acids in the flesh of these fish. There were no considerable differences in terms of weight gain, feed conversion ratio and uneven growth between the two groups, while survival was less likely among the the fish which had consumed more fat. We demonstrated, examining the flesh of the individuals which had consumed feed containing lower fat content, that it was fattier and contained more dry matter than those that had been fed of higher fat content. Besides, the content of omega-3 and omega-6 fatty acids was higher in the fish that had consumed less lipids.
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Differences in content of beet root and beet root juice, and it’s suitable to develop functional foods from animal sources
53-57Views:352In Western medicine is beginning to increasingly focus on the key role of healthy nutrition and lifestyle in preventing certain diseases. Therefore, it is important to emphasize the importance of primary prevention in our country, and to promote it; not only the health care team work, but broaden the recommended foods, which have benefit for human health as well.
Polyphenols are antioxidants, which are easily consumable with diet, however, food processing can influence the level of them.
In our study, nutritional assessment was made of raw beet root and a juice, made from this beet root. We investigated in which special diet referenced to eat this foods and whether this is suitable to develop functional foods from animal sources as a row material.
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Sunflower in Romanian agriculture
153-163Views:214Romania is one of the largest producers of sunflower closely followed by Bulgaria, Hungary, France and Spain. The total sunflower production increased after 1990, due the increase of cultivated surface during the moderate yield average increasing rate. Last year, Romania was ranked first in the European Union (EU) for the area cultivated with sunflower and the total production of sunflower seeds, according to the National Statistics Institute (INS). Romania's position on the map of sunflower producers area is supported by about 1 million hectares sown. In the segment of sunflower productivity, Romania has an unfavourable position, situated in the last places in the classification of the EU's most productive agricultures. In order to increase production per hectare, it is required to perform measures aimed at the elements of sustainable cultivation technology, including the stability of the production level by ensuring phytosanitary protection.