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Environmental Consequences of Efficient Use of Nitrogen Fertilizers
41-46Views:87Nitrogen fertilizer represents major economic burden. For this reason, although the efficiency of nitrogen utilization varies highly, its actual use generally remains at low levels; these averaging between 25 and 50%. We set up an experiment at the Oradea Research Station, using 15N labeled fertilizers, in order to investigate the possibility of increasing N fertilizer efficiency in winter wheat under irrigation conditions.
Fertilizers labeled with 15N allows us to individually determine its effect on yield formation, as well as the use efficiency of N from fertilizer following application rate and time. The amount of N derived from fertilizer as determined in straw and grain yield is high. When the labeled fertilizer is applied at tillering time, the values of this indicator rise when higher N levels we applied.
In separate experiments, we investigated a series of aspects connected to chemical fertilizer regarding the determination of the type of fertilizer, optimum time and rates of application; all these as a function of the special pedoclimatic conditions.
The results obtained in the field show that the effectiveness of N utilization in wheat is most variable and generally low, often ranging between 25 and 33%, owing to N loss within the system through leaching and NH3 volatilization.
A readily achievable increase in efficiency of 5 percentage points would result in considerable savings, and can be brought about by reducing nitrogen losses. The added benefits to the environment in terms of reduced ground/water contamination and lowered nitrous oxide (N20) emissions would also be substantial.
The figures for N fertilizer use efficiency (% N range from 35.5 to 72.6, the highest value being recorded with an N application of 120 kg/ha at tillering, when the previous crop was sunflower).
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Gene resources for improvement of drought tolerance and yield quality in dry pea breeding
105-111Views:109The effectiveness of selection for improved drought tolerance and consumption quality in the progeny of crosses between pea cultivars with semi-leafless (afila) and normal leaves and different origins, respectively, were investigated. After single crosses, parent cultivars and F1, F2 and F3 generations were grown under non-irrigated conditions in the same trials. We created a colour scale from 1 to 9 to measure statistically the shade of seed colour. The tolerance of genotypes against high temperature was measured by the number of pods per plant. The 3:1 segregation
observed in the F2 generation of crosses between semi-leafless and conventional cultivars indicated that the semi-leafless character is determined by a recessive gene. In contrast, the ratios of conventional (Af) and semi-leafless (af) genotypes were 7:1 and 9:1 ratio in the progenies of crosses of Af × af. The genetic progress was effective for improving the seed quality in F3 generation from crosses Af x af where we found that multiple
dominant alleles controlled the orange colour of cotyledons and its high heritability (h2 A=0,63). Selection is more effective in producing the genotypes with high yield and normal leaves if the crosses were made between the western European cultivars such as semi-leafless Profi and Delta used as maternal cultivars and conventional Auralia cultivars. In this case, there were decreases in the consumption quality, such as seed size and shade of colour. The selection based on the seed weight of single plants for increasing drought tolerance seemed to be more effective in F4
strains with normal leaves originated from Czechoslovakian maternal cultivar Y228; however, the genetic progress in the improvement of seed size and colour quality was slow. -
Some Aspects of the Relationship Between the Quality of Sheep Milk and Dairy Products
12-15Views:91The correlation between the quality of raw milk and the quality of milk products is evident. In spite of this fact we can find only a few references contained exact data related sheep milk. In recent paper we investigated the effect of SCC of sheep milk on the cheese yield (semi hard traditional cheese) and certain texture parameters of yoghurt from sheep milk. We wanted to know the relevant limit values of these properties for dairy applications.
In our opinion – in the case of the cheese yield – that the strong negative effect can be experienced when SCC is above 700.000-1 million/cm3.
In the case of yoghurt from sheep milk the limit values of SCC can be 1 million/cm3 for Adhesivity and Whey draining and 500.000/cm3 for Hardness. Considering these limit values in the selection of milk become materialise the highest quality of products and the economical production. -
Spatially Discrete GIS Analysis of Sampling Points Based on Yield and Quality Analysis of Sugar Beet (Beta vulgaris L.)
32-37Views:104Fulfilment of the increasing quality requirements of sugar beet production can be analysed with sampling of plants and soil at the cultivated area. Analyses of the spatial characteristics of samples require exact geodetic positioning. This is applied in practice using GPS in precision agriculture. The examinations were made in a sample area located in north-western Hungary with sugar beet test plant. According to the traditional sample taking procedure N=60 samples were taken in regular 20 x 20 m grid, where besides the plant micro and macro elements, the sugar industrial quality parameters (Equations 1-2) and the agro-chemical parameters of soils were analysed. Till now, to gain values of mean, weighted mean and standard variance values, geometric analogues used in geography were adapted, which correspond to the mean centre (Equation 3), the spatially weighted mean centre (Equation 4), the standard distance (Equation 5), and the standard distance circle values. Robust spatial statistical values provide abstractions, which can be visually estimated immediately, and applied to analyse several parameters in parallel or in time series (Figure 1). This interpretation technique considers the spatial position of each point to another individually (distance and direction), and the value of the plant and soil parameters. Mapping the sample area in GIS environment, the coordinates of the spatially weighted mean centre values of the measured plant and soil parameters correlated to the mean centre values showed a northwest direction. Exceptions were the total salt and calcium-carbonate contents, and the molybdenum concentration of the soil samples (Table 1). As a new visual analysis, the spatially weighted mean centre values of the parameters as eigenvectors were projected to the mean centre values as origin. To characterize the production yield, the raw and digested sugar contents of the sample area, the absolute rotation angles of the generated vectors were determined, which indicate numerically the inhomogenity of the area (Figure 2). The generated spatial analogues are applicable to characterise visually and quantitatively the spatial positions of sampling points and the measured parameters in a quick way. However, their disadvantage is that they do not provide information on the tightness and direction of the spatial correlation similarly to the original statistical parameters.
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Economic questions of maize production on different soil types
289-292Views:117The requirements and objective of cultivation are in constant change. For example, different cultivation systems are developed for the purpose of soil protection, the preservation of its moisture content and on soils with various precipitation supply or production site conditions. Traditionally, one of the most important cultivation aims is crop needs. Further cost saving in fertilisation and crop protection can only be achieved by reducing the quality and quantity of production or it cannot be achieved at all. Furthermore, the costs can be significantly reduced by means of the rationalisation of cultivation. Energy and working time demand can also be notably reduced if ploughing is left out from the conventional tillage method. The key requirement of economicalness is to perform the cultivation at the optimal date, moisture level and the lowest possible cost.
Within production costs, the cost of cultivation is between 3–17%, while they are between 8–36% within machinery costs. It is the vital condition the usability of each technological method to progressively reduce costs. Our evaluation work was carried out with the consideration of the yield data obtained from cooperating farms and the experiment database of the Institute for Land Utilisation, Regional Development and Technology of the Centre for Agricultural and Applied Economic Sciences of the University of Debrecen. Three technological methods (ploughing, heavy cultivator and loosening tillage) were used on several soil types which differ from in terms of cultivability (chernozem, sandy and sandy clay soils) from the economic/economical aspect. We examined the sectoral cost/income relation of maize production as an indicator plant. The maize price during the analytical period was 45 thousand HUF per t. On chernozem soils, the production of maize can be carried out on high income level, while maize production on sandy soils has a huge risk factor. The role of cultivation is the highest on high plasicity soils, since they have a huge energy
demand and the there is a short amount of time available for each procedure in most cases. -
Evaluation of striptillage and conventional tillage in maize production
37-40Views:167Tillage changes soil properties and the way how the environment affects those properties. Soil properties and environment determine the rate of water movement in liquid and gaseous form into and out of soil. Based on the experimental database of the Institute of Land Utilisation, Regional Development and Technology of the University of Debrecen, Faculty of Agricultural and Food Sciences and Environmental Management and the KITE PLC, various cultivation systems were examined with maize (Zea mays L.) as indicator plant in Jász-Nagykun-Szolnok county. The sample area can be found in the outskirts of Kenderes on a meadow chernozem soil. On the examined plot, strip-tillage, subsoiling and moldboard ploughing were performed, each on 4.5 ha, respectively. The purpose of the present study is to compare these cultivation systems according to the soil- and maize kernel moisture content and to the yield based on the years of 2012 and 2013.
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Response of sugar beet to different foliar fertilizers
127-136Views:123The yield and quality of the sugar beet are mainly determined by level crop production system; therefore the effects of foliar fertilization were studied. Our field trials were carried out in 2005 and 2006 in Hajdúböszörmény, at two experimental sites. In our trials the effects of Biomit Plussz, Fitohorm and Kelcare Cu (having high Cu content) as foliar fertilizers, as well as a fungicide with high sulphur content, Cosavet DF, were studied and
compared. Effects of treatments were studied in four replicates.
We took root samples at 4 week intervals, starting in August. The quality of root (sucrose, potassium, sodium and alfa-amino N content) was determined from filtrated beet broth, by an automatic beet laboratory system, called VENEMA. Leaf samples were taken three times in the growing season, element composition was measured by ICP-OES.
We found that the crop and the sugar yield were significantly influenced by the foliar treatments both of the two years. -
Change of cultivation parameters of asparagus (Asparagus officinalis L.) on different nutrient systems
67-71Views:153The experiments were conducted at the Research Institute of Nyíregyháza, Research Institutes and Study Farm, Centre for Agricultural Sciences, University of Debrecen. We have studied the shoot number, plant height and fold thickness os asparagus for 3 years. We have set a fertilization experiment on 1500 m2 of plantations in 2013. We applied fertilizer, manure and sheet manure compost. The set yield we achieved by using fertilizer treatment. The Vitalim produced the largest crop, then the Cumulus and the lowest yield Grolim. We was produced by highest green mass was observed in Vitalim and Cumulus hybrids in each year. The Grolim hybrid produced the smallest shoot and plant height but the fold thickness was longer in the other two hybrids. Overall, the Vitalim hybrid has the best production parameters and each season.
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Foliar treatments of Sugar beet
55-59Views:115The yield and quality of the sugar beet are mainly determined by the plant production system, thus we studied the effect of mineral fertilization. Our field trials were carried out in 2005 and 2006 in Hajdúböszörmény, at two sites. We studied the effect of sulphur (Cosavet DF), copper (Kelcare Cu) and two foliar nutrients with high active agents. Treatments were replicated four times.
We took root and leaf samples at 4 week intervals, starting in August. The quality of root (sucrose, potassium, sodium and alfaamino N content) was determined from filtrated beet broth, by an automatic beet laboratory system, called VENEMA. Leaf samples were measured with ICP-OES.
We found that the crop and the sugar yield were significantly influenced by the foliar treatments. -
Lessons of a stripe rust epidemic in a wheat fungicide trial in Debrecen
35-46Views:155During the 2014 year a fungicide application trial was made as a part of technology development in Debrecen (East-Hungary). Both in this trial and across in Hungary a serious stripe rust epidemic developed and caused great yield losses. The first sympotoms were observed in April on one of trial plots and during some following weeks a serious infection grown up. On the base of 13 fungicide active ingredients and dosages by two spraying applications, their efficacy could be evaluated in stripe rust control. Picoxystrobin 250 g/L acive ingredient applied in 0.6, 0.8 and 1 L/ha dosages (Acanto), picoxystrobin 200 g/L + ciproconazole 80 g/L combined active ingredients in 0.5, 0.75, and 1 L/ha dosages (Acanto Plus), epoxiconazole 83 g/L, moreover protioconazole 125 g/L + tebuconazole 125 g/L combinations gave good efficacy, respectively. The yield of well protected plots were 5 to 8 t/ha, but the losses were 40-70% when fungicides with no sufficient efficacy in stripe rust control were applied. The great infection, which was observed on different grasses late in the Autumn, 2014 might forecast a further stripe rust epidemic for the 2015 year.
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Polymorphism of the bovine GH and LEP genes in a population of Slovak spotted bulls
19-23Views:102The aim of this study was the detection of polymorphism in the bovine growth hormone and leptin genes using the PCR-RFLP method. A
polymorphic site of the growth hormone gene (Alul loci) that results in amino acid change at position 127 of the protein chain (leucine, L to
valine, V) has been linked to differences in circulating metabolites, metabolic hormones and to milk yield. The polymorphism in bovine leptin
gene is situated in the intron between two exons, which results in an amino acid change at position 2059 of the protein chain (cytosine, C to
thymine, T). The polymorphisms were studied in a group of 58 bulls of the Slovak spotted breed. A strategy employing PCR was used to amplify 428 bp (GH gene) and 422 bp (LEP gene) products from blood samples. Digestion of PCR products with restriction enzymes AluI and Sau3AI revealed alleles: L and V; A and B for GH gene and LEP gene, respectively. The growth hormone gene is a candidate gene for body weight gain in cattle, since it plays a fundamental role in growth regulation. Leptin plays an important role in the regulation of feed intake, energy metabolism, growth and reproduction of cattle; therefore, animals with higher leptin gene expression will probably have lower daily weight gain than others with similar forage offer and nutritional condition and will also likely have longer calving intervals. -
Organic soybean production in Hungary
45-52Views:187Organic soy production, particularly for feedstuff in organic animal husbandry, is needed in Hungary and the EU regions. Soybean producing crop area in Hungary has increased in the last years, however, the agroecological potential would allow for a larger soybean production area. This study presents the soybean market in Hungary, and the role of soybeans in protein supply in food and feed, summarising the critical elements of organic soybean production from variety selection to marketing. During the field research, the effects of the agronomical environment on yield potential factors were examined. We aimed to determine how different row spacing and tillage systems affect organic soybean yields of different varieties, with particular attention to the dry region, and determine the specific methods and varieties of soybean that favour these areas. We found that the tillage (plough/grubber), the spacing (wide/narrow), and the variety effects were all significant on morphological variables. The most remarkable difference was seen in plant height and the number of pods per plant, while the number of nodes was also highly impacted by tillage treatment and variety. The nutrition variables were significantly different, mainly as an effect of tillage and spacing interaction with significantly different plant responses of varieties.
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Possibilities of Growing Artichoke (Cynara scolymus L.) in Hungary
126-130Views:101The domestication of choice broadening, special vegetable species offers new market possibilities in Hungary. The production of artichokes has good perspectives due to its high protein, vitamin and mineral contents and its wide usability. According to the literature, in Hungary it is worth examining the annual production technology, which has yet to be researched in our country. The basic aim of our work was to establish the annual production of artichoke in Hungary with scientific research.
In the experiment, 5 Italian sorts (Valetta di Roma, Gobbo di Nizza, Violetto di Romagna, Violetto S. Francesino and Precoce di Romagna), 1 American sort (Green Globe) and 1 Egyptian sort (Balady) were used. For one half of the plants, we tried to bring on bud-production appearing in the first year only with early planting, while we used also a gibberellic acid hormone treatment for the other half.
Only the sort Green Globe had yields in appropriate quantity (21.4 t/ha) and quality. The low yield and inequable quality of buds of the Italian and Egyptian sorts refers to the fact that these sorts are not applicable for annual production in Hungary. Although the gibberellic acid treatment resulted an earlier harvest, increased the equality of buds and increased the proportion of stems producing buds except for the sort Green Globe, overall it did not have a positive effect. The hormone treatment fastened the physiological processes of the plants, thereby it induced early insenescence and lignifying of the buds. The high temperatures during the bud-formation period largely increased the ratio of flabby, over-blowing buds, so the planting must be timed in the future that the harvest period is in early automn or late spring, early summer months.
According to our results, globe artichoke can become a perspective culture by applying cold-resistant sorts with short growing seasons, which can be produced as annual crops without hormone treatment by the appropriate timing of bud-formation. -
The examination of the marigold’s (Calendula officinalis L.) nutrient requirement in small-plot trial
61-66Views:161During our research we investigated the marigold's (Calendula officinalis L.) nutrient requirements with different fertilization treatments in small-plot trial. We measured the harvested marigold's drug's raw and dry weight on a weekly basis from July 6th until August 17th. We were using SPME (Solid phase microextraction) and GC-MS (gas chromatograph-mass spectrometer) to examined the effects of the different fertilization settings for the herb's main active ingredients of essential oil's percentage.
It was concluded, based on the results, the N30P40K60 fertilization setting is ideal in terms of the quantity of the marigold drug. Both the raw and the dry weights' measurements of the case, this fertilization setting have the most important effect on the herb's yield. The analysis of variance didn't show significant differences between the plots with different fertilization settings. We discovered relationship between the drying loss and the increasing quantities of nutrients. We think it may be possible the Alpha-thujon's and Alpha-cadinol's production and the drying loss's data are connected, which appear to confirm the N15P20K30 treatment's data.
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Analysis of different fertilization settings’ effect in the case of the summer savory’s (Satureja hortensis L.) yield and active agents
101-105Views:176During our research we investigated the Mediterranean summer savory’s (Satureja hortensis L.) nutrient requirement with different fertilization settings in a small plot trial. We measured the medicinal plant’s raw and dry drug mass, and the presence of the main essential oil active agents under the different fertilization settings using SPME (Solid phase microextraction) and GC-MS (Gas Chromatograph mass spectrometer).From the drug’s raw and dry mass perspective, based on the obtained results, in our opinion, the N30P40K60 nutrient requirements were ideal in 2015, while the N60P80K120 nutrient requirement showed the best values in 2016. A significant relationship is assumed between the different fertilization settings and the essential oil active agents’ distribution. During the analysis of data, we compared three of the savory’s essential oil active agents’ percentage of presence in the nutrient requirement samples. -
Response of maize seedlings to the latent zinc deficiency under different Fe/Zn rati
125-128Views:101Zinc (Zn) deficiency is a critical nutritional problem for plants and peoples all over the world. Almost half of the world’s cereal crops are deficient in Zn, leading to poor crop yield. In this study, the effect of different Fe/Zn ratio on some physiological parameters of maize seedlings were investigated on the dry matter of shoots and roots and their ratio, SPAD index and the total length of shoots. The relative chlorophyll contents significantly decreased under increasing Fe given to the Zn deficient. The results showed that the different Fe/Zn supplies decreased the total length of shoots - ranging from 9% to 65% - by latent Zn deficiency. Corresponding to several scientific findings, it was observed that the non-optimal Fe/Zn ratio of tissues take part in the evolution of latent Zn deficiency in the case of high Fe concentration.
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Host plant preference of Metcalfa pruinosa (Say, 1830) (Hemiptera: Flatidae) in the north of Hungary
84-95Views:233Citrus flatid planthopper, a native insect to North America had for a long time a scarce economic importance there. However, being polyphagous made little damage on citrus trees and some ornamentals. In 1979 it was introduced to Italy where it established and spread quickly. It is now an invasive alien species continually spreading in South and Central Europe causing considerable damage in fruit crops and various ornamentals. Present study shows the results of a series of observations carried out from 2011 to 2015 at a number of habitats in north of Hungary. The pest could be found at each habitat but the hedge, the tree row, the gardens and the orchard/vineyard were the most infested. Frequency and population density of Metcalfa pruinosa were considerable on Asteraceae, Cannabaceae, Fabaceae, Juglandaceae, Lamiaceae, Rosaceae and Sapindaceae. Typical vegetation could be functionally classified as ornamental plants, trees/shrubs, fruit plants, weeds and feral plants. Feral plants – some of them also invasive alien species – were found at each habitat. Plant species native to America were among them the most populated. As the hedgerows were neglected, and most gardens, orchards and vineyards abandoned, these are excellent conditions for the quick and long-lasting establishment of the pest as well as they may be reservoirs to infest cultivated fruit crops and ornamentals. The hedgerow was situated along a railway line. The length of similar hedges can be merely in Pest county several hundred km, which means M. pruinosa has plenty of opportunity for spreading along the railway and infest agricultural and ornamental cultures. On the surveyed alfalfa and maize fields, accidentally very few nymphs and adults were observed. Although, the population density of M. pruinosa was considerable on many hostplants, economic damage or yield losses could not be detected. Economic or significant damage was observed only on roses, raspberries and stinging nettle. This later is cultivated in Germany and Finland. The applied horticultural oil was efficient.
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Changes in the Sulphur Content of Winter Wheat in a Field Experiment
85-88Views:86The use of superphosphate as P-containing fertiliser decreased in the last years in many countries in accordance with strict air pollution laws, and the S-deposition decreased from the atmosphere to the soil as well.
Winter wheat is the one of the S-demanding plants. Recently, the gradually increasing S absence endanger the formation of required average yield of winter wheat, and has bad effect on its quality.
We examined the effect of treatments on the sulphur-, nitrogen content and the N/S ratio of winter wheat in the whole upperground plant and in the grain and straw at harvest in a arable land sulphur fertilization experiment on brown forest soil (Agricultural Company of Felsőzsolca).
We analysed the samples from spring to harvesting, in the critical phenophases. In this study we discuss only the values from the stooling and stalking and the results of analysis of grain and straw in the harvest.
We experienced that the concentration of sulphur in the whole upperground parts of winter wheat showed increase to the end of vegetation independently of fertilization. The N/S ratio was between 8% and 12% in the beginning of the growth period in the whole upperground plant, while the ratio in the grain at harvest was between 13 and 14%. When we examined the whole upperground plant, stalk and leaf at stalking, we got the highest sulphur content in the leaf. Mostly the middle level sulphur fertilization dose (4 l/ha) increased the sulphur accumulation in the green plant. At total maturing, the greatest part of accumulated sulphur is in the grain, but then the effect of fertilization is less glaring. -
The examination of relation between the C/N ratio and the biogas yields in the Regional Biogas Plant of Nyírbátor
63-68Views:114The Regional Biogas Plant of Nyírbátor was built by the Bátortrade Ltd. The biogas plant contains 6 mezophil and 6 thermophil fermentation tanks, because the biogas production is based on mixed compositions. The regional plant is a multifunctional system. It produces agricultural products and biogas with high methane content. The utilization of biogas is also accomplished here; gas-engines transform it to electricity and
heat-energy. The product electricity is used by the local plants, the surplus is sold.
The aim of the research is the examination of the quality and quantity of the input materials that put into the mixers and follow the seasonal, periodical and optional changes of the input materials. The analyzation of the quality and quantity data can give an answer to the optional changes of biogas production because the input materials determine the composition of the examined recipe. The C/N ratio was between 11-13, the maximal value of the biogas yield was observed by 12.35 C/N ratio. -
The effect of increasing compost rates on the yield and nutrient content of ryegrass (Lolium perenne L.)
127-134Views:137Satisfaction of the increasing needs of humanity causes large environmental load. To provide a livable environment for future generations we have to satisfy our needs with the use of sustainable management. This is one of the biggest challenges of nowadays.
The amount of wastes emitted in increasing volume can be decreased by the recycling of them. The disposal of waste materials formed in the public spaces of cities and during the processing of agricultural row materials and by-products in landfills is inconceivable, so they must be recycled.
These materials mostly with organic compounds could be the primary substrates of composts. Completed with suitable additives, and applied appropriate treating technology, composts are capable to supply horticultural plants with nutrients. Composting wastes and byproducts not just decreases the amounts of deposited waste materials, but increases the nutrient (macro- and micronutrients) content of soils, so this is an environmentally friendly and alternative way of nutrient management of plants. -
A földhasználat alakulása az ezredfordulón - egy felmérés eredményei
58-66Views:92There were no significant changes in land ownership and use in 2000, compared to the period following the compensation process. Land is mainly owned by people who do not want to use their property, so they generally turn to renting it out to others. Although farming on rented land is performed under various conditions, farms try to rent the lands of better quality. In general, examined farms would like to increase their size by tenancy or land purchase. Buying land is a good investment, considering the price increase in the future, but because of the lack of capital, tenancy remains the main form of territory increase.
Farms which rent land are in connection with a lot of owners, the land within their use is frittered away, it is in a lot of parts. Most rental contracts are written, but the ratio of oral contracts is still quite high. To reduce this ratio is one future aim. One more characteristic of the rental contracts is the dominance of a medium length period – which is acceptable for both the owner and user, but the ratio of short time contracts is still high. Land rent, on average for the examined farms, is quite balanced, it is on the level of about 16.000 HUF/hectare.
The land users mainly pay the same land rent to the owners, but in some cases, there are exceptions. Generally, land rent is a fix cost, but sometimes this cost depends on the level of the yield or the change of product price. Land rent, on average, is 16% within the production costs on the examined farms, which try to choose better and larger lands for rent. Sometimes, farmers can pay higher land rent for the unit of better land, but this is not a general tendency. It is mainly true that they pay the same land rent for the unit, regardless of land quality. Land owners can not interfere in the use of their land within the rental period, and also is a main characteristic of contracts that important particulars are not spelled out sufficiently by the partners. It follows that their content has to be improved in the future. -
Qualitative detection of genetically modified organisms in plant samples
309-313Views:145We analysed the GMO content of corn samples by polymerase chain reaction following the appropriate optimization of the reaction. The analysis included two main steps: extraction of DNA from the sample, and detection of the GMO content by polymerase chain reaction. The polymerase chain reaction is an in vitro method to multiply chromosomatic or cloned DNA (cDNA) sequences through the enzymatic pathway. The reaction is sensitive enough to produce DNA in sufficient amount for the analysis from a single DNA. We identified the PCR products by agarose gel electrophoresis. When optimizing the reaction, the MgCl2 concentration, reaction time and temperature have to be taken into consideration. The temperature of the anellation has to be increased until the highest specificity and yield is reached. If the temperature of the anellation is too high, the primer is linked to non-specific sites as well; in the gel visualization, more lines can be seen at one sample. If the temperature of the anellation is too high, the primer is insufficiently linked or is not linked at all (too few lines in the gel visualization). After optimization, the GMO content in the unknown sample can be determined along with the appropriate positive and negative controls.
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Sour cherry anthracnose and possibilities of the control with special regard to resident Glomerella population in sour cherry plantations of East Hungary
12-17Views:150Anthracnose is considered one of the most destructive diseases for sour cherry production due to the rapid development of the disease on fruits. Glomerella cingulata (Stoneman) Spauld. & H. Schrenk (anam.: Colletotrichum gloeosporioides (Penz.) Penz. & Sacc. in Penz.) has been the fungal pathogen responsible for anthracnose in last decades. Yield losses greater than 90% may occur under epidemic conditions. C. acutatum (J.H. Simmonds, 1968) strains were isolated of sourcherry plantations in East Hungary and this pathogen, new for Hungarian microbiont became recently dominant. Contrarily to the former species it is certainly transmitted with ants during fruit ripening. About third of strains proved to be cutinase producers that enable them to actively penetrate via cuticule, and these strains infect directly berries of blackberry, grape and tomato as well as plum and apple. Most of cutinase negative strains could also infect these fruits after mechanic injury. All strains of both species produce amylase, cellulase, lecithinase, lipase, polyfenoloxydase and protease in vitro, although the activity of these enzymes highly varied in the medium. The only C. acutatum strains produced noticeable amount of chitinase. Strains, tolerant to recently applied fungicides to control the anthracnose, could be isolated of sour cherry plantations that might be the cause of ineffectiveness of control measures in 2010. The mycofungicide containing mixture of three Trichoderma species in oil carrier could efficiently depress the development of anthracnose in ripening sour cherry.
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Estimation of the vine-shoot yield in the Gyöngyös district
21-26Views:129Every year, a significant amount of vineyard prunings is generated in Hungary. Instead of utilization them, it is burned directly on the field in spite of the limitations of legalization highly pollute the environment in recent decades. The vineyard pruning itself or with other by-products from pomology and forestry can be significant amount of fuel source. However, before the planning to utilization the essential task is to estimate the potential. This study examine that how many vineyard is generated in the micro-region of Gyöngyös in every year on appointed vineyard.
In order to vine-branch potential calculate more accurate data was necessary so the products had to be determined for all plant. Thus, the biomass resource and the utilization become plannable. As the result of the experiment we can estimate that 1,5 t/ha vineyard pruning are generated. It means more than 9 thousand tons pruning in the micro-region of Gyöngyös. This amount can be convert into fuel source more than 150 thousand GJ heating value.
The results evince that the vineyard pruning is important fuel source in every year in the micro-region of Gyöngyös. Further usage is suggested for other regions in other size.
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Effect of harvest on the different fertilization methods of asparagus (Asparagus officinalis L.)
33-37Views:153The experiments were performed in the University of Debrecen Centre for Agricultural Research Institute of Nyíregyháza of 1500 m2 asparagus plantation in 2011. We were determind the number of shoots, plant height, fold thickness and yield of asparagus hybrid. I studied to the effects of various cropyear is addition Grolim hybrid asparagus growth and development. Compared to previous years, 2014 was unusually wet and cold weather conditions which causes the plantation has developed outstanding. The soil temperature early and dynamic increase due to, the harvest had started a one month earlier 2014 than the 2013. The experiment asparagus harvested from lower yields as a result of the extremely dry spring in 2015.