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Azoxystrobin resistance of Botrytis cinerea Pers.:Fr. isolates
56-63Views:108Fungicide resistance is one of the most important problems endangering the effectivity of practical plant protection today. The frequent and subsequent usage of specific fungicides results the emergence of resistant fungal populations. This threatens is especially high in case of Botrytis cinerea Pers.:Fr. being an endemic pathogen with frequent infection. Nowadays the main method of protection as against Botrytis cinerea is the application of chemical fungicides chemicals. Therefore, a better knowledge of local populations is necessary for the planning of the protection procedures.
Based on the results of our examinations we may establish that the growth of the examined samples showed a significant difference under in vitro circumstances, which shows a great deal of variability of the Botrytis cinerea populations in Hungary. Twenty-five Botrytis cinerea samples from different hosts were analyzed in this study. High resistance was found towards azoxistrobin in seven cases, and low resistance in eight cases.
It was also proved, that the B. cinerea is able to bypass the inhibition site of the azoxistrobin via the alternative oxidase. The presence of this altermative mitocondrial electrotransport route considerably reduces the effectivity of the chemical. -
New approach in soil tillage – bases of the precision crop production
123-127Views:127A new approach is needed in soil tillage practice. The important achievements of this are the recognition of the risk – poor tillage practices, poor soil quality, soil state defects, and climate extremes etc. – the need for the development of risk reduction, prevention, remediation and maintenance of the favorable soil state. In this paper 13 main soil state defects are listed, to which the prevention and improving tasks are also commented. In the second part of the paper the most important soil tillage tasks are summarized in 30 sections and realization of these points may promote the implementation of precision plant production.
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László Szabó herbologist awarded by „Antal Gulyás medallion for crop protection“ in 2014 (laudation)
5-7Views:266The Public Utility for Development of Crop Protection Teaching (NOFKA) and The Hajdú-Bihar County Regional Association of Hungarian Chamber of Crop Protection Specialists and Plant Doctors (Chamber) established a joined Award Committee in 2011, which intend to serve as moral appreciation to prominent persons with excellent achievements by awarding the „Antal Gulyás medallion for crop protection” which are available for outstanding teachers, researchers, and practical crop protection specialists.
In 2014 László Szabó herbologist has been decorated with the „Antal Gulyás medallion for crop protection” for his “excellence in applied herbology and practical research activity on herbicide applications, moreover for knowledge transfer on weed management”.
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Examining reaction of sunflower genotypes on planting time on chernozem soil
93-99Views:122We studied the effect of planting time on plant pathological factors, leaf area index and yield production by applying various fungicid treatments on two different sunflower genotypes in 2013.
By delaying planting time, both the extent of Diaporthe, Alternaria and Phoma infections decreased. The differences between the volume of infections were significant in the case of the early and late sowing time results. The application of fungicide treatments induced a notable decrease in the extent of infections for all three pathogens examined. The LAI-values varied between 0.3 and 5.6 m2/m2 in 2013 depending on the hybrid, sowing time and treatment. Stocks planted at distinct times reached maximum leaf coverage at different times. The planting time and the fungicide treatment had a significant effect on the formation of the leaf area. In the case of average and late planting times, fungicide treatments elongated the preservation of the green leaf area.
With respect to the yield amount, average planting time (27 April, 2013) turned out to be optimal in 2013 (control – NK Ferti: 4.621 kg ha-1, PR64H42: 4.196 kg ha-1; double-treated: NK Ferti: 5.282 kg ha-1, PR64H42: 5.090 kg ha-1). Fungicide treatments resulted in significant yield growth in all cases during our research.
We applied Person correlation analysis to evaluate the hybrids’ sensibility to infections and our results varied in the case of Diaporthe and Phoma (r=-0.343*, -0.379**). Infections of the three pathogens were significantly reduced by delaying the planting time and applying fungicides. Late sown stocks preserved the green leaf area for a longer period. Besides, the application of the fungicide treatment and the hybrid itself also led to the preservation of the green leaf area. However, pathogens examined notably decreased the leaf area by the end of the growing year. The fungicide treatment had a remarkable effect on yield growth (r=0.603**). Furthermore, the presence of higher LAI-values in the period prior to August also induced higher yields.
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Improved soil and tomato quality by some biofertilizer products
93-105Views:283The use of microbial inoculums is a part of sustainable agricultural practices. Among various bioeffectors, the phosphorus-mobilizing bacteria are frequently used.
The objective of this study is to investigate the effect of some industrial biofertilizer inoculums, of containing P-mobilizing bacteria on the quantity and some quality parameters of tomato fruits. Spore-forming industrial Bacillus amyloliquefaciens FZB42 (Rhizovital) as single inoculums and combinations with other Bacillus strains (Biorex) were applied on Solanum lycopersicon Mill. var. Mobil test plant. Soil microbial counts, phosphorus availability, yield and fruit quality, such as total soluble solids (TSS) content and sugars (glucose, fructose) were assessed. The results found that single industrial inoculums of FZB42 product had positive effect on P-availability and fruit quality in the pots. Fruit quality parameters, TSS content, soluble sugars were significantly improved (p<0.05). Such better fruit taste was correlated significantly by the most probable number (MPN) microbial counts. Use of such bioeffector products is supported by the positive interrelation among measured soil characteristics and inside healthy quality parameters of tomato fruits.
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Effect of net shading technology on the yield quality and quantity of chilli pepper under greenhouse cultivation
5-9Views:317This study was undertaken to identify the effect of net shading technology on the total capsaicinoids, vitamin C and relative chlorophyll content expressed as SPAD (Soil Plant Analysis Development) values and total marketable fruit yield of ‘Star Flame’ chilli pepper (Capsicum annuum) for two harvesting times cultivated under modified atmosphere. ‘Star Flame’ pepper was grown under three (3) different net shading colours (white, red and green). Samples without net shading were used as control. Samples were subjected to chromatographic analyses using HPLC for the determination of total capsaicinoids and vitamin C. Interaction in shading technology and harvest times (p=0.010) had significant effect on total capsaicinoids as a result of green and white shading technologies showing higher differences when compared to control samples. Vitamin C content was observed to have increased in white and red shadings (p=0.001) after the first harvest and gradually decreased after the second harvesting time (p=0.002). Relative leaf chlorophyll content was significantly higher in white shading in the first and second harvest. Samples used as control had significantly higher marketable values when compared to white and green shadings with red recording low marketability of ‘Star Flame’ chilli peppers.
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Investigation of combining ability and superiority percentages for yield and some related traits in yellow maize using line × tester analysis
5-14Views:251Combining ability estimation is an important genetic attribute for maize breeders in anticipating improvement in productivity via hybridization and selection. This research was carried out to investigate the genetic structure of the 27 F1 maize hybrids established from nine lines derived from Maize Research Department and three testers, to determine general combining ability (GCA), determine crosses showing specific combining ability (SCA) and superiority percentages for crosses. Nine lines, three testers, 27 F1 hybrids and two check commercial hybrids (SC162 and SC168) were studied in randomized complete block Design (RCBD) with three replications during 2016. The results of mean squares showed that significant and highly significant for most studied traits (days to 50% tasseling, days to 50% silking, plant and ear height, ear position, ear length, no. of kernels per row, 100-kernel weight and Grain yield). Estimates of variance due to GCA and SCA and their ratio revealed predominantly non-additive gene effects for all studied traits. Lines with the best GCA effects were: P2 (line 11) and P6 (line 21) for grain yield, for testers Gm174 and Gm1021 had significant GCA effects for grain yield. The hybrids P5×Gm1021, P6×Gm1021, P7×Gm1021, P8×Gm1002, P9×Gm1002 had significant and negative SCA effects for grain yield. Crosses P1×Gm174, P2×Gm1002, P5×Gm1021, P6×Gm174, P6×Gm1021, P7×Gm1021, P8×Gm1002, P9×Gm1021 were the best combinations manifested and significant superiority percentages over than check varieties (SC162 and SC168) for most studied traits. Therefore, these hybrids may be preferred for hybrid crop development.
Abbreviations: GCA general combining ability; SCA specific combining ability
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Effects of Site on Winter Wheat Quality 2002/2003
100-107Views:140The demand of modern societies for high food quality is evident. Thus, it is important for agriculture to produce row materials that are valuable for nutrition and have favourable characteristics for food processing. For this we need a knowledge about the factors which determine the quality of products. One of the main features of plant production is the “immobility”. This way the characteristics of the field influence the quality of the product, like example winter wheat, which is the main cereal in Hungary and Europe.
The Concordia Co. has charged the Central Laboratory of Debrecen University, Agricultural Centre with laboratory testing of the 2002/2003 winter wheat crop. The samples consist of thirteen winter wheat varieties from six different sites under the same cultivating conditions. Therefore, the important wheat quality factors were analysed solely against site conditions with the use of Győri’s “Z” index, which contains these parameters.
Soils were tested first. In this experiment excepting the negligible differences between the sites, there were no linear relations found between quality factors, productivity and soil features. The case is the same with the relation between precipitation, temperature and quality parameters. However, it must be noted that additional soil analyses are required to interpret the extreme results obtained from Karcag.
The calculated Győri’s Z-index shows relative stability concerning certain varieties, although considerable deviation can be found in varieties related to the sites. According to these results, it can bestated that winter wheat quality was not linearly influenced by soil and weather in the 2002/2003 vegetation period. As the same cultivation technology was used in the experiment, the index was determined by genetic features. It must be noted that these findings are relevant only to this experiment. -
Increase of Wheat (Triticum aestivum L.) Resistance to Leaf Rust (Puccinia tritici) via Gene Transformation
127-129Views:81Leaf rust is one of the most significant fungal disease of wheat not only in Hungary but also in other parts of the world. For improving leaf rust resistance of winter wheat variety (Hajdúság, 2003) produced by conventional breeding methods, verified by results of variety tests, showing outstanding results in the aspect of the most important economic values, integration of tissue culture technics, genetic engineering and traditional
methods may provide facilities. Building the gene(s) responsible for resistance into the determined genome can improve the resistance in a way that changes other features of the plant slightly or not at all. In the course of genetical transformation of the variety Hajdúság we applied one of the wheat’s own effecient green-tissue specific insurer genetical regulator, the promoter of ribulose carboxylase 1-5 bisphosphate (RuBisCo) ‘s small
subunit to control the expression of the gene cmg1. -
Testing of paraffin oil efficiency against grape powdery mildew in Eger wine region
73-80Views:410The aim of the present study was to examine the efficiency of paraffin oil against powdery mildew in Eger wine region. The experiment has been carried out in 2013 and 2014 with Chardonnay and Kékfrankos grape varieties, which have different resistance against powdery mildew. The effectiveness of the oil was examined on leaves and clusters (frequency and intensity). This oil was effective against Erysiphe necator infection on field trials in Chile and Brazil. The spread of downy mildew (Plasmopara viticola) was also inhibited by this material in some experiments conducted in Spain and France.
The differences between oil treatments represented the sensitivity of the grape varieties in accordance with the applied dosages. The oil was effective against powdery mildew with different extent as a result of the so called ”vintage effect”. In 2013, the treatment of the highest dosage (D3) didn’t differed significantly in frequency and intensity of infection from the regular treatment (clusters of Kékfrankos, leaves of Chardonnay). In 2014, the oil was not so effective against powdery mildew compared to 2013. No remarkable differences were detected between the treatments due to the strong pressure of powdery mildew. Furthermore, no any effect of the lowest dosage (D1) was detected in the case of the sensitive clusters of Chardonnay and leaves of Kékfrankos in both experimental years.
In summary, the oil treatment has an effect against powdery mildew, however this efficiency largely depends on the vintage characteristics and the pressure of powdery mildew. Further investigations are neccessary, for example field trials with combinations of other sprays. The oil can be useable as fungicide with proper care in eco-friendly integrated and bio (ecological) viticulture.
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Comparative study of special honey products and herbhoneys
117-120Views:390Honey has a positive effect on human body due to its high content of biologically active substances (e.g. monosaccharides, vitamins, enzymes, amino acids, polyphenolic compounds). The properties of honey depend on its botanical origin due to the bioactive plant components, mainly secondary metabolites that are included in honey made by bees from nectar. Herbally infused honeys are delicious products that combine the therapeutic action of herbs and honey. Additionally, herbs can provide nutrient fortified syrup for honey bees and protect them against diseases and other ecological threats.The aim of this study was to define the physicochemical properties of multifloral honey, herb enriched natural honey and herbhoney samples. We measured the moisture content, pH value, electrical conductivity and proline content. Although great diversity was observed in the basic properties of the examined products. In our study, we found that the electrical conductivity shows the significant differ between the groups. All the samples, including the herbhoneys passes the quality standards of honeys. -
Agronomical and Economic Evaluation of Different Soil Cultivation Systems
255-258Views:84In the interest of profitable plant production and environmental protection we have to make an effort to protect and improve the productivity of our soils while moderating production limiting factors. Due to different soil cultivation methods, the quantity of yield and required expenses also differ.
We examined the production costs in four different production technology systems. Overall, it can be said that farming standards are good, since cost prices were low (2001: 14-15 HUF/kg, 2002: 15-21 HUF/kg, 2003: 39-49 HUF/kg) in the case of all main products per 1 kg. Cost prices were lowest in the case of direct sowing, probably due to low machinery costs.
All economic indicators have to be compared when choosing the most suitable production technology in a specific farming environment. -
Qualitative detection of genetically modified organisms in plant samples
309-313Views:178We analysed the GMO content of corn samples by polymerase chain reaction following the appropriate optimization of the reaction. The analysis included two main steps: extraction of DNA from the sample, and detection of the GMO content by polymerase chain reaction. The polymerase chain reaction is an in vitro method to multiply chromosomatic or cloned DNA (cDNA) sequences through the enzymatic pathway. The reaction is sensitive enough to produce DNA in sufficient amount for the analysis from a single DNA. We identified the PCR products by agarose gel electrophoresis. When optimizing the reaction, the MgCl2 concentration, reaction time and temperature have to be taken into consideration. The temperature of the anellation has to be increased until the highest specificity and yield is reached. If the temperature of the anellation is too high, the primer is linked to non-specific sites as well; in the gel visualization, more lines can be seen at one sample. If the temperature of the anellation is too high, the primer is insufficiently linked or is not linked at all (too few lines in the gel visualization). After optimization, the GMO content in the unknown sample can be determined along with the appropriate positive and negative controls.
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Activity of some enzymes, participating in nitrogen compounds transformation in chernozem, polluted by fluorine compounds
99-104Views:117Contamination of chernozem by fluorine compounds variously affects those enzymes (urease, asparaginase, glutaminase, arginase, amidase), which takes part in the metabolism of nitrogen-bearing organic compounds. In broken soils the inhibited desaminisations is stronger, than enzymatic hydrolysis of asparagine and arginine. The features of seasonal dynamics of change activity of urease and correlation dependence of its activity from some physical and chemical soils properties are described. These tendencies well comport with the results of model experiments. At minimum HF influence there is inhibition of processes of monohydrocarboxylic acids desaminisation, hydrolytic breaking up of arginine and glutamine. By a side with this there is activating of urea and asparagine breaking up processes on the initial stages of toxicant influence. The study of kinetics of process of urea enzymatic hydrolysis in chernozem at the different level of HF influence showed changes of initial and maximal velocity of enzymatic reaction, and also Michaelis-Menten constant.
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The effect of different compost rates on the yield of ryegrass (Lolium perenne L.)
95-98Views:133Protection of natural resources and sustainable natural resources management are essential for the long-term survival of humanity. This makes necessary nowadays the development of environmentally conscious living and spread of that in the future. The amount of organic waste materials, produced during human activities, could be decreased by composting instead of dispose them in landfills. Applying appropriate treatment technology and additives, the compost could be used as fertilizer for horticultural crops and it could increase the easily available nutrient content of soils. Compost utilization prevents nutrient deficiencies and by using the optimal rate, we could reach significant yield increases.
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75 years of the chestnut blight fungus Cryphonectria parasitica (Murr.) Barr in Europe
82-85Views:237The chestnut blight fungus Cryphonectria parasitica is a native pathogen in East Asia and has been introduced into North America and Europe. Historical records and population genetic studies revealed at least three major introduction events from Asia into Europe.
Nowadays, chestnut blight is present in almost the entire distribution range of European chestnut, i.e. from the Iberian Peninsula to the Caucasus. The C. parasitica population in most countries has been studied in respect to the diversity of vegetative compatibility (vc) types and the occurrence of hypovirulence. The vc type diversity of the different populations varied considerably. Typically, a high diversity of vc types has been found in areas with a long history of chestnut blight and where sexual recombination between divergent genotypes commonly has occurred. On the other hand, newly established populations often showed a low diversity with only one, or a few vc types present.
Hypovirulence, i.e. the occurrence of C. parasitica isolates infected by Cryphonectria hypovirus 1 has been found widespread in Europe. Natural dissemination and active biological control applications have lead to a high prevalence of the hypovirus and to the recovery of many chestnut stands. Virulent cankers became hypovirus-infected within a short time and ceased expansion. There is concern that the diversity of vegetative compatibility types could increase in Europe through sexual reproduction between C. parasitica genotypes originating from different introductions. A higher level of vegetative incompatibility would not only hamper hypovirus spread within a population but could also select for lower virulence in CHV-1 and subsequently lead to an erosion of biological control. Recent studies, however, indicate that the vc type barriers are not so restrictive than previously assumed and that so far no evidence for an erosion of biological control system in high diversity populations can be observed.
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Relationship between the change of soil moisture content of different soil layers and maize yield
19-25Views:186The development of chernozem soil water management and its relationship with maize yields was studied in a 30-years long-term field experiment with different crop-rotation systems (mono-, bi- and triculture), in three crop years with different natural precipitation: a drought (2007), a wet (2008) and a dry (2009 one. The relevant soil layer was divided to three sub-layers: (0–60 cm, 61–120 cm, 121–200 cm) in which the development of soil moisture content was investigated during the whole vegetation. From the results it can be stated that change of the water stock of the upper soil layer (0–60 cm) was the most intensive. Both the direct effect of natural precipitation and irrigation could be observed in the most obvious way in it. Yield result of maize and the highest water supply deficit values in the vegetation were compared in our work too. According to the results it was revealed that among the three studied crop rotation systems it was the monoculture, the success of production of which depends the most of water supply. The most favourable crop rotation system was the triculture from both the aspect of the yield of produced crops and the favourable soil properties too.
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Nutritional disorders of arable crop growth in eastern Croatia
273-290Views:154Nutritional imbalances accompanied with growth retardation of crops at early growth stage were found since the last 40 years on certain arable lands in eastern Croatia. In this regard, phosphorus (P) deficiencies in maize and wheat were found mainly on acid soils of the western part of the region, potassium (K) deficiencies in maize, soybean on the hydromorphic neutral to alkaline calcaric drained gleysols of Sava valley lowland, while zinc (Zn) deficiencies were observed mainly in seed-maize and soybean on neutral calcaric eutric cambisols of the eastern part of the region. Cold and moist spring is factor promoting P deficiency symptoms. As oasis of normal crops existed on same arable land, comparison of plant and soil composition was possible from typical sites. P nutrition disorders were in connection with the lower P and the higher aluminum (Al) and iron (Fe) concentrations in the top of plants and the lower soil pH values. K-deficiency as result of strong K fixation and imbalances with high levels of magnesium (Mg) were the main responsible factors of low maize and soybean yields on some drained gleysols. Chlorosis incidences typical for Zn deficiency in maize and soybean were in close connection with the higher soil pH, the lower quantities of mobile Zn, here and there the higher mobile P in soil, the lower concentrations of Zn and the higher levels of Al and Fe in plants. Overcoming the above mentioned disorders and normalization of yields were achieved using ameliorative fertilization either by K or P fertilizers and in case of Zn by foliar spraying of crops with 0.75% ZnSO4 solution. Also, alleviations are possible by selection of more tolerant genotypes of field crops to specific types of nutritional disorders. From this aspect, some practical solutions were recommended for maize with reference to K nutritional problems.
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The effect of various composts on vegetable green mass on two soil types
179-183Views:180Composting of sewage sludges makes easier the utilization of sewage sludge in the agriculture and the composts in good quality could increase the nutrient content of soil. Due to the composting process, the sewage sludge composts with high organic matter content can be utilized in the same way as other composts or farmyard manure.
Composts produced in different ways have different effects on the physical, chemical and biological properties of different soils, although their positive effects have already proved in the literature. In our study the effects of composts from different composting processes were investigated in soil-plant systems. The different physical and chemical properties of the two examined soil types (arenosol and chernozem)strongly influenced the nutrient supply capacity of composts which could be characterized by the growth of ray-grass as a test plant in the pot experiment. In this work we examined the effects of three different composts on the green weight of plants on the fourth and eighth weeks after the treatment and sowing. -
Influence of temperature and variety on seed germination of soybean (Glycine max L. Merr) at different germination times
5-12Views:156Seed germination is an important stage in crop development that affects plant performance, crop yield, and quality. Many factors influence seed germination, and one of the most important factors is temperature. The closer the temperature is to the optimum, the faster germination will occur. Temperature affects seed germination in various plants and varies depending on the variety. Therefore, the effect of temperature on seed germination is necessary to investigate, also for soybean varieties. Soybean is one of the world's most valuable oil-seed crops. Generally, proper seedling establishment and germination of soybean seeds are crucial processes in the survival and growth cycle of the crop. Thus, a study was done to investigate the influence of temperature and variety on soybean seed germination at different times after sowing. The experiment was carried out at the Institute of Agronomy, Hungarian University of Agriculture and Life Sciences (MATE), Crop Production Laboratory. Seeds of two soybean varieties were subjected to three different temperatures: 15, 25, and 35 °C, and two soybean varieties, Martina and Johanna were tested. There were four germination times based on the days after sowing: Days 3, 5, 7, and 9. This experiment was set up with a completely randomized block design and four replications. According to the findings of this study, the Martina variety showed a better germination rate as well as higher vigor and viability than the Johanna variety in the first 12 days after sowing. At temperatures of 15 °C and 25 °C, both varieties achieved comparable percentages of viability, but they were more vigorous at 25 °C due to better total seedling length. Thus, the information gained from this study will indirectly determine and confirm the proper temperature for the initial growth of the Martina and Johanna varieties.
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The olive (Olea europaea) and the almond (Prunus amygdalus) related phytonutrients, and the associated health-promoting biological effects, a review
11-24Views:262With the increasing attention to the health promoting activities of the bioactive compounds from some plants, many researchers are focusing on the biological potential and mechanisms of certain cultivated plant species. In this review, we survey the olive and almond based extracts specific phytoconstituents and their associated health promoting effects that have been evaluated in experimental and clinical studies.
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From Organic to Precision Farming (Contemporary Publication)
81-86Views:79The paper presents a short review of the different types of farming systems:
Biofarming, Organic farming, Alternatíve farming, Biodynamic farming, Low input sustainable agriculture (LISA)
Mid-tech farming, Sustainable agriculture, Soil conservation farming, No till farming, Environmentally sound, Environmentally friendly, Diversity farming
Crop production system, Integrated pest management (IPM), Integrated farming, High-tech farming
Site specific production (SSP), Site specific technology (SST), Spatial variable technology, Satellite farming.
Precision farming
It concludes that the various systems are applicable in different ratios and combinations depending on the natural and economic conditions.
The author predicts an increase in precision technologies , the first step being the construction of yield maps compared with soil maps and their agronomic analysis. Based on this information, it will be necessary to elaborate the variable technology within the field, especially for plant density, fertilization and weed control.
The changes in weed flora during the past fifty years based on 10.000 samples within the same fields using the weed cover method are presented. -
Effect of Irrigation on Maize Yield (Zea mays L.)
30-35Views:362We have been continually examining the fertilizer and irrigation reaction of commonly cultivated maize hybrids for nearly twenty years at the Látókép experimental station of the Center for Agricultural Sciences, Debrecen University.
Upon evaluating the results, it can be established that year significantly influences the size of yield. Between the years of 1999 and 2002, in the average of applied fertilizers the difference is 3,4 t/ha, but even in irrigated treatments it reaches 3 t/ha. This is more than the effect of irrigation. Of the applied agrotechnical elements, the yield increasing effect of fertilization is the greatest and can even be greater than the effect of year. The yield increasing effect of fertilization can be reliably detected with small and medium doses, but at higher doses a plateau section is reached, where it is not worth applying more nutrients. Cultivation with irrigation can only be done with appropriate nutrient supply, due to the positive correlation of the two factors. The positive correlation also means that if the water supply of the plant declines, less fertilizer is needed for safe production. The two factors (irrigation and fertilization) have to be increased or decreased at the same time.
According to the experiment, in unirrigated treatments, 90 kg/ha nitrogen and the related phosphorus and potassium are enough, while in irrigated treatments this was 120 kg.