No. 38 (2010)
Articles

Activity of some enzymes, participating in nitrogen compounds transformation in chernozem, polluted by fluorine compounds

Published November 3, 2010
Vitalii Gryshko
Plant Physiology and Soils Biology Department, Kryvyi Rig Botanical Garden NAS of Ukraine, 50, Marshaka str., Kryvyi Rig, 50089, Ukraine
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APA

Gryshko, V. (2010). Activity of some enzymes, participating in nitrogen compounds transformation in chernozem, polluted by fluorine compounds. Acta Agraria Debreceniensis, (38), 99–104. https://doi.org/10.34101/actaagrar/38/2767

Contamination of chernozem by fluorine compounds variously affects those enzymes (urease, asparaginase, glutaminase, arginase, amidase), which takes part in the metabolism of nitrogen-bearing organic compounds. In broken soils the inhibited desaminisations is stronger, than enzymatic hydrolysis of asparagine and arginine. The features of seasonal dynamics of change activity of urease and correlation dependence of its activity from some physical and chemical soils properties are described. These tendencies well comport with the results of model experiments. At minimum HF influence there is inhibition of processes of monohydrocarboxylic acids desaminisation, hydrolytic breaking up of arginine and glutamine. By a side with this there is activating of urea and asparagine breaking up processes on the initial stages of toxicant influence. The study of kinetics of process of urea enzymatic hydrolysis in chernozem at the different level of HF influence showed changes of initial and maximal velocity of enzymatic reaction, and also Michaelis-Menten constant. 

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