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Role of Sowing Time in Maize Production (Review)
36-39Views:78Many authors, both in Hungary and abroad, have reported on experiments carried out to determine the role of sowing time in maize, but the results are often contradictory. This is hardly surprising, since the maize plant exhibits enormous genetic variability and the hybrids created through selection and inbreeding may have very specific requirements as to sowing date. The year effect, too, often complicates the efforts of scientists to provide clear guidance to farmers on the optimal sowing date for each hybrid.
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Effect of NPK fertilization on the yield and yield stability of different maize genotypes
101-104Views:138The yielding capacity and quality parameters of 11 maize hybrids were studied in 2011 on calcareous chernozem soil in a 25-year long-term fertilization experiment in the control (without fertilization), in the base treatment of N 40 kg ha-1, P2O5 25 kg ha-1, K2O 30 kg ha-1 and in five treatments which were the multiplied doses of the base treatment. The N fertilizer was applied in the autumn and in the spring, while P and K fertilizers were applied in the autumn.The sowing time was 17–18 April, the time of harvest was 8 October. The 30-year average of precipitation (April–Sept) was 345.1 mm, the amount of precipitation did not differ greatly from that, however, its distribution was very unfavourable.
It was found that the largest yield increment (as compared to the control) was in the treatment N 40 kg ha-1, P2O5 25 kg ha-1, K2O 30 kg ha-1 in the long-term experiment. The largest yields were obtained for the hybrids P9494, PR37N01 and PR35F38 (13.64–13.71 t ha-1). Due to the dry period at the end of the summer – beginning of autumn, the grain moisture content at harvest was favourably low, 12–18% depending on the treatment and the growing season.The N fertilization significantly increased the protein content of the kernel, but the starch content of the kernel decreased (significantly in several cases) with increasing fertilizer doses and yields as compared with the control.
The highest protein content was measured in hybrids GK Boglár and Szegedi 386. The oil content was above 4% for GK Boglár, but the two hybrids were not among the best yielding hybrids in spite of their good inner content. The starch content was around 75 % without fertilization, it decreased with fertilization.
For the tested hybrids, the fertilizer dose N 120 kg ha-1, P2O5 75 kg ha-1, K2O 90 kg ha-1 can be recommended with respect to efficacy and environmental considerations. -
The effect of agrotechnological factors and the cropping season on sweetcorn (Zea Mays L. convar. saccharata Koern.) production in a humid year
146-151Views:75We have examined the effect of three agrotechnological factors (sowing time, fertilization, crop density) and four genotypes on the yield
of sweetcorn on chernozem soil in the Hajdúság region in 2009. The experiment was set up at the Látókép Research Site of the University of
Debrecen. We have included two sowing times (27 April, 26 May), six nutrition levels (control, N30+PK, N60+PK, N90+PK, N120+PK,
N150+PK) and four genotypes (Jumbo, Enterprise, Prelude, Box-R) at two crop density levels (45 thousand ha-1).
In the humid cropyear of 2010 the amount of precipitation exceeded the 30-year average by 184 mm in the cropping season; the average
temperature exceeded the same by 0.8 C on the average of the examined months. The circumstances were most favourable for sweetcorn
production with the first sowing time, thus, this was when the yield of all hybrids was the highest.
With early sowing time, the highest yield (23437 kg ha-1 yield) was obtained with Enterprise at 45 thousand ha-1 crop density level at
N150 + PK nutrition level. The highest yield of the other three hybrids was 22253 kg ha-1 (Jumbo) 22286 kg ha-1 (Box-R) and 1873 kg ha-1
(Prelude). With the second sowing time, the highest yield was obtained with Enterprise again (22237 kg ha-1) at 65 thousand ha-1 crop
density level. With this sowing time the yield of Jumbo, Box-R and Prelude was 20888 kg ha-1, 17796 kg ha-1 and 17401 kg ha-1, respectively.
We found that the highest yield was obtained at the highest nutrition levels (N120 + PK, N150 +PK) with the first sowing time, while the same
was obtained at lower nutrition levels (N90 + PK, N120 + PK) with the second sowing time. -
Evaluation of long term experiments from a new aspect
55-60Views:119During our work, we developed a new, simple method to show the effects of fertilization on yield, which can both be applied over the long term as well as in series of independent experiments.
During the testing of this method, at the experimental farm of the Debrecen University Center for Agricultural Sciences at Látókép on a chernozem soil with lime deposits, we examined the fertilizer reaction of maize hybrids between 1989 and 1994. The treatments were: winter tillage, plant density of 70-80 thousand, unfertilized, N 120, N 240 kg/ha fertilized treatments, long term experiments using Dekalb 524 and Volga SC hybrids in long term experiments.
Four parameters are shown in the model. In the examined period TRmax represents the greatest yield in the fertilized treatments, NT the yield in unfertilized treatment, k the „efficiency of fertilizer” to NT and b the depression-coefficient, where the expected value is zero. The expected grain yield of the fertilized treatments (Y), in the function of the unfertilized grain yield (x) is the following:
The parameters were determined using the Monte Carlo method, in the optimizing process the sum of deviation square was minimized. The correct conformation of the functions was determined by the greatness of the R-value and the standard error. We found that during six years of testing, the tendency of fertilization efficiency was similar in the case of both hybrids. There was an unfavorable weather interval and, in these years, the yields were low, fertilization did not have an effect and moreover, in extremely bad conditions resulted in an obvious yield decrease. With the improvement of conditions, which in the case of our country means an increase in precipitation, the efficiency of fertilization increases and reaches its peak at 13-14 t/ha. At this point, the yield increasing effect of fertilization is 4-4,5 t/ha. If the yield of the unfertilized treatments increases from 8-9 t/ha, then the efficiency of the applied fertilizer decreases.
Most likely, the k and b parameters depend on the soil of the experimental location (nutrient and water management) and on the amount of pplied fertilizer and the characteristics of the hybrid. With the increase of fertilizer dosage the k-parameter also increases. The greater value though does not obviously mean a more favorable situation. It is true that in medium and good years this means great fertilizer efficiency, but in low or extreme precipitation conditions it also means greater risk. With the increase of the k-parameter, the yield deviation also increases which, from a cultivation point of view, is quite unfavorable. If the value of the b-parameter is other than, zero then the effect is clearly unfavorable, because with the increase of this value, the yield decrease is also greater. The fertilizer reaction of the two examined hybrids can be well characterized by these two hybrids.
Examining the six years, our created model estimated the effect of fertilization on the yield accurately and with a high degree of safety. Both in highly unfavorable and extremely good years, it gave an exact estimate. In our opinion, it can be used well to evaluate the effects of fertilization on yield in the future. -
The effect of water supply and crop year on the yield potential of sweet maize (Zea mays L. convar. saccharata Koern.) hybrids with different genotypes
203-210Views:173The successfulness of crop production is significantly affected by not only the the average yields that provide cost effectiveness, but also the success of striving for yield safety, therefore, varieties and hybrids tolerant to environmental stress factors are worth being included into the sowing structure. Our aim was to further the decision making of producers in prepaering the right sowing structure by the evaluation of sweet maize hybrids’ tolerance to excess rainfall.
We performed our examinations in an extremely wet year (2010) on chernozem soil on three sweet maize hybrids (GSS 8529, GSS 1477, Overland) in 12 replications. Comparing the yields of 2010 with those that can be expected under optimal rainfall conditions, we showed that the examined hybrids react to the amount of rainfall higher than their needs with yield depression. The excess rainfall tolerance of the examined hybrids is different in the case of each hybrid. -
Impact of environmental changes resulting from different sowing dates on maize yield
99-104Views:140Three Debrecen maize hybrids of different genotypes (Debreceni 285, Debreceni 377 and Debreceni 382) were examined on chernozem soil in a field experiment. During the two years of the experiment (2009–2010), we wanted to get to know how the examined hybrids reach to different sowing dates and what impact early, optimal and late sowing has on yield.
In 2009, balanced soil and air temperature resulted in steady emergence. However, the low temperature in early April and the cooling down in mid-May 2010 caused a delayed emergence.
The grain moisture content at harvesting and the high yield showed a strong crop year effect. In 2010, yield was much lower (1.664 t ha-1) and grain moisture was significantly higher (34%)than in 2009.
In 2009, early sowing resulted in yield decrease (P<0.05), but it also significantly reduced grain moisture at harvesting (P<0.05). Although late sowing slightly increased yield (not significantly), but grain moisture at harvesting increased by 9.2%. In 2010, optimal sowing date was shown to be the best alternative from the aspect of yield, but there was no significant difference in comparison with early and late sowing. Grain moisture at harvesting greatly increased (13.3%).
The Debreceni 382 maize hybrid reacted to sowing dates flexibly, neither early, nor late sowing affected its yield significantly and the grain moisture at harvesting showed 12% increase in the case of the late sowing date. In 2009, maize hybrids Debreceni 285 and Debreceni 377 reached their highest yield in the case of the sowing date which was shown to be optimal (23rd April), while the different sowing dates had no effect on yield in 2010.
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Impact of tillage systems on maize emergence
129-136Views:63In Europe, there has been a significant change in the way tillage is approached in recent years. This change is due to a growing awareness among farmers, politicians and society as a whole that soil is not a renewable resource in itself. From an agricultural point of view, the greatest impact on soil condition can be achieved through the use of the applied tillage systems. My research takes this approach as a basis when examining the different tillage systems and their impact on the environment. In this context, conventional and a variety of no-tillage systems are examined in this paper. As a next step, it is examined how the environmental conditions created by the different tillage systems influence the emergence of maize hybrids. The analyses are carried out in a multi-factorial, long-term tillage field experiment. The same batch of the same hybrid seed was sown in several crop years, and the effects of environmental conditions on the emergence process were examined. Environmental effects and emergence-related uptake were measured in the examined plots. Measurements of environmental effects included air temperature, precipitation, soil temperature measured at seeding depth, as well as % cover of stem residue on the surface in the treated plots. The first emergence time measurements of the sown crop in the plots of each treatment were compared and relationships between these factors were investigated.
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Comparison of Variability among Irradiated and Control Inbred Maize Lines via Morphological Descriptions and Some Quantitative Features
70-73Views:66Knowledge of genetic diversity in breeding material is fundamental for hybrid selection programs and for germplasm preservation as well. Research has been done with nine irradiated (fast neutron) and four non-treated inbred lines. The aims of this study were (1) to investigate the degree of genetic variability detected with morphological description (based on CPVO TP/2/2) in these materials, (2) to compare the genetic changes among irradiated and non-irradiated maize inbred lines (based on some quantitative features). The irradiation did not change any of the characteristics clearly in positive or negative way, which can be related to the fact that the effect of induced mutation on genetic structure cannot be controlled. From the irradiated lines we have managed to select plants with earlier ripening times and better phenotypes. We could distinguish 3 main groups by the morphological features; these results match our expectations based on pedigree data. Markers distinguishable on the phenotypic level (e.g. antocyanin colouration, length of tassels) were significant in all lines.
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Influence of H-FABP gene polymorphisms on slaughter value of hybrid pigs
55-60Views:229The H-FABP gene was defined as a potential candidate gene influencing the fat deposition traits, primarily the intramuscular fat content. The aim of this study is to define whether the previously reported gene mutations are connected with the slaughter traits measured in a standard slaughterhouse. The study included data from 405 gilts and barrows from 2 different samples. The two chosen mutation (HFABP1: c. 103 T>C, HFABP2: c. 1970 T>C) were detected in one reaction with PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Lenght Polymorphism) method with HinfI restrictoin enzyme. The allel frequencies are as follows: 103T(H)=0.75; 103C(h)=0.25, 1970T=0.32; 1970C=0.68. A HFABP1 mutation has significant effect on backfat thickness and lean meat % at stable 1 (sample 1), but there were no effect at stable 2 (sample 2). The analysis of values of production traits, depending on HFABP2 genotype did not reveal significant differences. Based on this study we can’t get a clear conclusion on the impact of polymorphisms on production parameters. In the examined flock the allele frequency of mutation in 5 'UTR is identical to the literature data, i. e. the more favorable variant regarding the intramuscular fat content is predominant in the population.
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Grain Moisture of Maize Hybrids in Different Maturity Groups at Various Harvesting Dates
24-25Views:85The experiments were designed to determine the extent to which late harvesting helped to achieve low grain moisture content. The grain moisture contents of 24 hybrids from each of four different maturity groups were recorded during the last decade of September and the first decade of November over a period of three years (1999-2001).
The data indicated that late harvesting led to a substantially smaller difference between the hybrids. While in late September the difference between the grain moisture content of the earliest (FAO 200) and latest (FAO 500) hybrids was 8.9%, this value dropped to 1.5% over the average of three years when measurements were made in early November. With the exception of the earliest group, the grain moisture content in all the maturity groups declined during October. The later the hybrid, the greater the decline.
This change in the grain moisture content during October exhibited a considerable year effect. When the weather in October was warm, with little rain, the decrease was greater, while in cool, wet years the grain moisture content declined to a lesser extent, or in some cases even increased. -
Micropropagation of Leuce-poplar clones and its role in selection breeding
43-48Views:162Leuce-poplars (mostly white poplar and its natural hybrid grey poplar) are native tree species trough Hungary. They are covering more than 4.0 per cent of the Hungarian forested area (77 000 ha). The white (grey) poplars play a significant role in the forestation under sandy soil site conditions as well as they are of importance from nature conservation point of view as well.Long-term selection breeding work is going on at the National Agricultural Research and Innovation Centre Forest Research Institute (NARIC FRI), involving selection of fast-growing Leuce-poplar clones under dry site conditions.Micropropagation technology is relatively quickly spreading in forestry. In vitro multiplication of trees is applied mainly in fruit growing in Hungary, in case of forestry it is used mainly for selection breeding.This paper presents a short overview on the micropropagation trials with different Leuce-poplar clones and the early evaluation of the seedlings growing of the micropropagated clones/varieties. -
Grain yield and quality of maize hybrids in different FAO maturity groups
126-131Views:68An improvement in the quality of maize grain by increasing the level of components responsible for its biological value is possible
by using genetic means. However, a change in the genotype, together with improving the nutrient properties of the grain, also has some
adverse consequences connected with a fall in yield and in resistance to diseases.
Field experiments were conducted during three years (2003, 2004and 2005) to evaluate environmental effects on grain yield and
quality responses of maize hybrids. Twenty one hybrids of various maturity groups (FAO 150-400) were planted to achieve an optimum
(60-70 000 plants per hectare) plant populations and grown under the medium-N (80 kg N ha-1) fertilization. Environmental conditions
significantly affected maize hybrid responses for grain yield, starch, oil and protein contents, and consequently, starch, oil and protein
yields per hectare. Hybrids of flint type, which have a short vegetation period, had high protein and oil content but the yield averages
were low due to the slower rate of starch incorporation. Hybrids of the dent type have a longer growing season and more intense
carbohydrate accumulation, but low protein and oil contents. In wet years there was a higher rate of starch accumulation, while dry
years are favorable for protein and oil accumulation. Positive correlation existed between starch content and grain yield and 1000-
weight as well as between oil content and volumetric weight among tested hybrids. Negatively correlation existed between grain oil and
starch content as well as between oil content and grain yield and 1000-weight. Thus, end-users that require high quality maize may need
to provide incentives to growers to off set the negative correlation of grain yield with oil and protein content. -
Comparative analysis on the fertiliser responses of Martonvásár maize hybrids in long-term experiments
111-117Views:78The results of experiments carried out in the Agricultural Research Institute of the Hungarian Academy of Sciences clearly show that in the case of hybrids grown in a monoculture greater fertiliser responses can be achieved with increasing rates of N fertiliser than in crop rotations. In the monoculture experiment the parameters investigated reached their maximum values at a rate of 240 kg/ha N fertiliser, with the exception of 1000-kernel mass and starch content. In both cases the starch content was highest in the untreated control, gradually declining as the N rates increased. Among the parameters recorded in the crop rotation, the values of the dry grain yield, the 1000-kernel mass, the protein yield and the starch yield were greatest at the 160 kg/ha N fertiliser rate, exhibiting a decrease at 240 kg/ha. Maximum values for the protein content and SPAD index were recorded at the highest N rate. It is important to note, however, that although the N treatments caused significant differences compared to the untreated control, the differences between the N treatments were not significant.
In the given experimental year the values achieved for the untreated control in the crop rotation could only be achieved in the monoculture experiment at a fertiliser rate of 160 kg/ha N, indicating that N fertiliser rates could be reduced using a satisfactory crop sequence, which could be beneficial from the point of view of environmental pollution, crop protection and cost reduction.
The weather in 2006 was favourable for maize production, allowing comparative analysis to be made of the genetically determined traits of the hybrids. Among the three hybrids grown in the monoculture experiment, Maraton produced the best yield, giving maximum values of the parameters tested at a fertiliser rate of 240 kg/ha N. The poorest results were recorded for Mv 277, which could be attributed to the fact that the hybrid belongs to the FAO 200 maturity group, while the other hybrids had higher FAO numbers. Maraton also gave the highest yields in the crop rotation experiment at the 160 kg/ha N level. All three hybrids were found to make excellent use of the natural nutrient content of the soil.
It was proved that the protein content of maize hybrids can only be slightly improved by N fertilisation, as this trait is genetically coded, while the starch content depends to the greatest extent on the ecological factors experienced during the growing season. -
Evaluation of dry matter accumulation of maize (Zea mays L.) hybrids
35-41Views:369The increase of the grain yield of maize is closely correlated with its seasonal dry matter accumulation. Dry matter is accumulated into the grain yield during the grain filling period. The following maize hybrids were involved in the experiment: Armagnac FAO 490, Loupiac FAO 380 and Sushi FAO 340. In order to determine dry matter content, two samples per week were taken on the following days: 22nd, 25th, 28th, 31st August, 4th, 7th, 14th, 18th, 22nd, 25th, 29th September and 2nd, 6th, 9th, 13th October. In the course of sampling the weight of 100 grains from the middle section of 4 ears was measured in 4 replications. Dry matter content was determined after drying to constant weight in a drying cabinet at 60 °C. Harvesting was performed on 13th October 2017.
The daily precipitation sum was determined by local measurements, while the daily radiation and temperature data were provided by the Meteorological Observatory Debrecen of the National Meteorological Service in Budapest. Among the agrometeorological parameters, an analysis was made of the precipitation during the growing season, effective heat sums during the vegetative and generative phase, and the water supplies. The daily heat sums were determined using the algorithm proposed.
The amount of precipitation in the winter period before the 2017 growing season was 210 mm. The soil was saturated until its field capacity. The rather dry and warm March and April had a favourable effect, but there was no worthy amount of precipitation until May (51 mm) due to the condition of the dried seedbed. Sowing was performed on the 5th of May 2017 in a randomised small plot experiment. There was favourable precipitation and temperature during the growing season, thereby providing ideal conditions for maize development, growth and yield formation. There was near average amount of precipitation in each year. The total amount of precipitation in the summer period is 342 mm. Temperature was mostly above the average, but there was no long and extremely warm period.
The Armagnac hybrid reached its highest dry matter mass 126 days after emergence. Physiological maturity was reached sooner (on the 119th day) in the case of Loupiac, and even sooner in the case of Sushi (116th day). The thousand grain weight of Sushi (which has the shortest ripening period) was 286 g at the time of physiological maturity, while that of Loupiac was 311 g. Compared to Sushi, Armagnac showed 12 g more dry matter accumulation (306 g). In the case of all three examined hybrids, physiological maturity was preceded by an intensive phase, when the dynamics of dry matter accumulation was rather quick. On average, Sushi gained 2.8 g dry matter per day between 103 days following emergence and physiological maturity, while the same values were 3.2 g for Armagnac and 3.3 g for Loupiac. The aim of the regression line slope is to predict the behavior of the dependent variable with the knowledge of the values and characteristics of the independent variables using the regression line equation. Furthermore, to determine how the location affected the dynamic of dry matter accumulation in the Armagnac, Loupiac and Sushi hybrids. In regression analysis, the coefficient of explanation showed that the effect of day in the Armagnac was 97%, in the Loupiac 94%, in the Sushi 90 %. The determination coefficient (R2) is useful in determing how the regression equation fits. But, as we have seen, the determination coefficient alone is not sufficient to verify the model’s accuracy, in addition to the determination coefficient (R2), the normality of the data or the residuals, the variance of the variables at different levels, the independence of the data relative to time and non-oblique. Observations are evaluated for the correctness of the fitted model.
Dry matter values decreased evenly and slightly following physiological maturity. According to our research results, it was established that physiological maturity is followed by a moderate dry matter loss. Until harvesting, Armagnac lost 40 g of its thousand mass weight in 29 days, while the same value pairs were 69 g in 36 days for Loupiac and 29 g in 39 days for Sushi. Loupiac – which had the highest weight at the time of physiological maturity – lost the most of its dry weight; therefore, Armagnac and Sushi had higher values at the time of harvesting.
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Correlation between sowing time of maize hybrids, yield and seed moisture content at harvest on chernozem soil
32-41Views:114In this paper, we analysed the results of maize sowing time experiments conducted by the Department of Crop Sciences and Applied Ecology of the University of Debrecen Agricultural Sciences Centre, during the period from 1997-1999. We made the experiments at the experimental garden of DE ATC, on a chernozem soil with lime deposits.
In 1997, we examined five hybrids, in 1998 six hybrids, and in 1999 three hybrids, with three sowing times. Sowing times were early (10. Apr. and 08. Apr.), optimal (25. Apr. and 28. Apr.) and late (15. May and 17. May).
We examined the following standards: yield, seed moisture content at harvest, thousand kernel mass, duration of flowering, emergence time and profitability.
In 1977, the emergence times, in order of sowing, were: 24, 12 and 9 days. Yields of the sowing times were the following, in mean, for the five hybrids: in the early sowing time (10. Apr.) 11,81 t/ha, in the optimal sowing time (25. Apr.) 11,67 t/ha, and in the late sowing time (15. May) 12,9 t/ha. The seed moisture content of the five hybrids at harvest was 8% less in early sowing time, than in the late sowing time. The thousand kernel mass was the biggest in late sowing time, but we could not prove any significant connection attributable to the effect of sowing time. We examined
profitability, too. Of the five hybrids, four attained the greatest profit with the early sowing time in 1997.
In 1998, the emergence times, in the order of sowing, were: 21, 10 and 11 days. Yields of the sowing times were the following, in mean, for the six hybrids: 08. Apr. 10,34 t/ha, 25. Apr. 11,02 t/ha, 15. May 11,52 t/ha. There were no significant differences between yields in 1998. The seed moisture content of the six hybrids at harvest was 7% less for the early sowing time, than for the late sowing time. In 1998, the profits were greatest for the
early and traditional sowing times.
In 1999, the numbers of days from sowing to emergence were 18, 9 and 9 days, in the order of sowing times. Yields of the sowing times were the following, in mean, for the three hybrids: 13,25 t/ha, 12,51 t/ha and 12,34 t/ha, in the order of sowing times. The seed moisture content of maizes at harvest was 6% less with an early sowing time in the mean of all hybrids. In 1999, hybrid maizes gave big profits with early sowing times.
Summing up the results of the three years, we can conclude that we get a significant yield increase and reduced seed moisture content at harvest if we apply the early sowing time, which can considerably increase the efficiency of maize cultivation. -
Study of drought stress correlation on yield and yield components of maize cultivars (Zea mays L.)
67-73Views:188This article was investigated to study the correlation and analysis of drought stress regression on maize cultivars' yield and components. The variance analysis results showed a significant difference between drought stress levels in terms of plant height, total dry weight and number of seeds per row, the total weight of cob, grain yield, harvest index, stem diameter, and cob weight with protective leave. Also, there was a significant difference in ear weight without protective leaves, ear diameter, ear length, plant weight, 100-seed weight, and seed per ear on hybrid treatments. There were statistically significant differences between cultivars in plant height, leaf area, ear diameter, ear length, number of seeds per row, number of seeds per ear, the total weight of cob wood, 100-seed weight, harvest index, plant dry weight. The results of the correlation of traits for the mean levels of drought stress showed a positive and significant correlation between plant yield and plant height, seed per row, ear length and weight of 5 pieces of wood and also with a total weight of cob wood, ear weight with bark showed the highest correlation. There is a significant correlation between leaf area and stem diameter, plant weight, total dry weight at the probability level of 0.05. Correlation coefficients between traits in non-stress conditions showed a positive and significant correlation between grain yield and height, ear length and grain in the row, which was a significant increase compared to stress conditions. The correlation of traits under full stress conditions also showed that the correlation coefficient between cob length trait and positive height was positive and significant. From the study of correlation coefficients between maize traits in non-stress conditions, it can be concluded that the most important components of grain yield are cob length and grain per row. While the correlation coefficients under moisture stress conditions show that the grain trait in the row has a positive and significant correlation with yield, under stress conditions in the cob stage did not show any traits with correlation yield.
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Development of an InDel marker set to establish hybridization between wild boar and domestic pig (Sus scrofa) breeds
21-25Views:274Wild boar and domestic pig breeds belong to the same species (Sus scrofa), so they can easily have viable offspring. This could be a problem in preserving the genetic lines of wild boars, keeping clean the food industry from lower-grade hybrid boar meat, and „producing” ethically questionable trophies, too. The aim of our study was to develop a cost-efficient, fast, easy and accurate marker set which can separate the wild boars from hybrids and domestic pig breeds.
The InDel markers were developed using 59 full pig genomes of 17 different breeds (e.g. Duroc, Large White, Landrace, Mangalica, wild boar). Sequence differences between the genomes of wild boars and domestic breeds were identified in variant call files, and verified using the IGV software. Wild boar, mangalica and duroc specific primers to amplify the chosen InDel regions were designed using Primer3.
After preliminary tests five markers were chosen, three wild boar specific, one Mangalica specific and one Duroc specific one. Fluorescently labelled primers were used to make the valuation easier and more accurate with capillary electrophoresis instead of gel-electrophoresis. The markers were optimised individually and in multiplex conditions and tested in samples of 11 breeds.
In conclusion, a new, faster and cheaper set was developed to separate the wild boars from the hybrids and domestic breeds. Based on the preliminary testing on wild boars, duroc and mangalica breeds zero samples resulted false negative, so it is 100% accurate. In addition, it is a much more cost- and time-effective way than testing every single sample with STR sets.
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Production of corn in Serbia in the light of climate change
305-322Views:196This paper presents the basic elements of maize production technology in Serbia. For their good knowledge, it is necessary to be familiar with the basic biological requirements of the species, phenology and its particular requirements for water, heat, soil conditions and mineral nutrition. A special emphasis in this paper is given to recent research by the author regarding the correction of production technology (selection of hybrids, time and density of sowing, etc.) from the aspect of knowing the weather conditions of the year to the moment of sowing and the amount and position of available nitrogen in the soil profile during the spring before sowing. In the light of predicted climate change conditions for the Republic of Serbia, the authors of this paper have proposed and appropriated adaptation measures. The general conclusion is that there are no general recipes for the correct production technology of this plant species, but should be adapted to each specific year, field and hybrid.
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Molecular Biological Approach of Crocus sativus L. and its Allies
112-115Views:150The hay saffron (Crocus sativus L.) is a sterile triploid plant, known in human culture only, with no fertile seeds produced. The origin of saffron is still a mist, however it is assumed to be an autopoliploid mutant or a hybrid. The recent classification and most of the former taxonomic publications define C. sativus to be derived from C. cartwrightianus, a wild species. Because of the sterility of hay saffron it seemed to be reasonable to apply molecular biological methods to complete classical taxonomic studies in examining its relations. The DNA polymorphism based AFLP method has confirmed the close relationship between these species.
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Genetic diversity study of Hungarian honey bee (Apis mellifera L.) colonies – Previous announcement
61-64Views:162In Hungary, the Apismelliferacarnica is the native breed which is the only recognised and breedable honey bee in the country. It is assumed that there are a number of non-native and hybrid honey bee breeds in Hungary. The microsatellite and mitochondrial DNA surveys applied here will be utilised to easily and accurately identify the various sprads, and open up new ways in the research of honey bees. The isolation of the genomial DNS from 5 to 7 day old larvae samples was successfully carried out. In the future the plan is to carry out the measure with microsatellite markers. As an initial step the optimal annealing temperature was identified. In the mitochondrial DNA survey the COI-COII mytochonrial regional primer due to its low anneling temperature cannot be used with any normal sequencing methods. By using these method the aim of this research is the measurement of genetic variance.
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Study of plant physiological parameters in winter oilseed rape (Brassica napus var. napus f. biennis L.) production on chernozem soil
111-115Views:200We made plant physiology examinations in Arkaso winter oilseed rape hybrid substance: relative chlorophyll content (SPAD) and leaf area index (LAI) measurements. The experiment was set in University of Debrecen Agricultural Sciences Center at Látóképi Experimental Station in four replications, in two different sowing times (I. sowing date on 08/22/2014 and II. sowing date on 09/09/2014 sowing againhappened because of the incomplete germination in the second subtance 01/10/2014) Three different plant density 200, 350 and 500 thousand ha-1, under the same nutrient supply, 45 cm row spacing. The experiment was green crop of winter wheat. The relative chlorophyll content (SPAD) and leaf area index (LAI) measurements were made in seven different times. We measured the maximum value of chlorophyll content in the first sowing time at 500, and the second sowing time at 350 thousand ha-1 plant density. The measurement results proved that there was a linear relationship between the number of plants and the LAI. The maximum leaf area index values we measured in both the sowing time at 500 thousand ha-1 reached.
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Evalution of energy for bioethanol production
77-80Views:120The objective of this study was the ethanol which classified as agro fuels. The aim of our research was the calculation of efficiency of bioethanol production, and evaluates the yield of maize hybrids grown for this purpose. We examined the energy demand of corn production per hectare in two vintages of 2009 and 2010. The focus of the experiment was placed in three different doze of fertilizer. Results show that the control corn plot used the least amount of non-renewable energy. Improving starch yield by adding fertilizer required additional nonrenewable energy inputs. So then the invested energy has a great impact on the efficiency.
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Andrological examination of Hungarian Large White and Landrace boars
5-10Views:217The Hungarian Large White and Hungarian Landrace pig breeds have outstanding lifetime performance, production parameters and crossbreeding ability. Nevertheless, during the recent decades, these breeds could not compete with the West European hybrid pigs concerning on production results. In this study, we made a complex andrological evaluation of boars in different Hungarian nucleus breeding stocks. After taking blood and semen samples, performing gonadotropin-releasing hormone (GnRH) challenge, ultrasonic and thermographic examinations were done. Laboratory tests were accomplished in reproduction labs of NARIC ABNMS. Our goal was to determine the reproductive performances of boars and collect samples for future genetic examinations, respectively.
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Volume of Paulownia Shan Tong (Paulownia fortunei × Paulownia tomentosa) plantation in Eastern Hungary: a case study
43-46Views:157Volume tables for tree plantations are not unknown in international practice. In many places, this is due to the uniqueness of the species or variety composition of the plantations and the cultivation technology used. In most cases, this is also justified by specific soil (ecological) conditions. In Hungary, publications on Paulownia have not yet included a volume table. This is the first one we are publishing, thus it can be considered as a gap-filler. The research was conducted in Monostorpályi, a 1.8 hectare, 8-year-old municipal plantation. 8 trees were selected randomly and their parameters were studied.
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The hydric stress influence on quantityand quality of the maize yield in the Crisurilor Plainconditions
21-27Views:112The paper is based on the researches carried out in an experiment placed on the preluvosoil from Agricultural Research and Development Station Oradea, Crisurilor Plain during 2008–2011in the following variants: V1=Irrigated, without irrigation suspending; V2=Irrigated, irrigation suspending in May; V3=Irrigated, irrigation suspending in June; V4=Irrigated, irrigation suspending in July; V5=Irrigated, irrigation suspending in August; V6=Unirrigated. The hybrid used: Fundulea 376. In the variant with optimum irrigation, water reserve on 0–75 cm depth was maintained between easily available water content and field capacity. Pedological drought was determined every year and the irrigation was also needed. The irrigation determined the increase of the total water consumption and yield gain in comparison with unirrigated variant. Irrigation suspending in different months determined the yield losses very significant statistically. The biggest protein content was registered in the variant without irrigation suspending; the values registered in the variants with irrigation suspending in May, June, July and August and in the unirrigated variant are smaller, with differences statistically assured. There was a direct link between de Martonne aridity index values and water consumption, yield and protein content and an inverse link between pedological drought and yield quantity and protein content. These are the arguments for irrigation opportunity in maize from Criurilor Plain.