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Influence of H-FABP gene polymorphisms on slaughter value of hybrid pigs
55-60Views:635The H-FABP gene was defined as a potential candidate gene influencing the fat deposition traits, primarily the intramuscular fat content. The aim of this study is to define whether the previously reported gene mutations are connected with the slaughter traits measured in a standard slaughterhouse. The study included data from 405 gilts and barrows from 2 different samples. The two chosen mutation (HFABP1: c. 103 T>C, HFABP2: c. 1970 T>C) were detected in one reaction with PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Lenght Polymorphism) method with HinfI restrictoin enzyme. The allel frequencies are as follows: 103T(H)=0.75; 103C(h)=0.25, 1970T=0.32; 1970C=0.68. A HFABP1 mutation has significant effect on backfat thickness and lean meat % at stable 1 (sample 1), but there were no effect at stable 2 (sample 2). The analysis of values of production traits, depending on HFABP2 genotype did not reveal significant differences. Based on this study we can’t get a clear conclusion on the impact of polymorphisms on production parameters. In the examined flock the allele frequency of mutation in 5 'UTR is identical to the literature data, i. e. the more favorable variant regarding the intramuscular fat content is predominant in the population.
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Simplified volume equations for grey poplar (Populus × canescens Smith.) standing trees
27-31Views:357Grey poplar (Populus× canescens Smith.) is a natural hybrid of white poplar (Populus alba L.) and Eurasian aspen (Populus tremula L.). It can be found throughout Europe, where both parents are present. The above mentioned species of poplars (under the term of ‘domestic poplars’) cover approximately 5% of the forests of Hungary. Of these species, grey poplar holds significance in forestry, and its role in afforestation shows a growing tendency. For this reason, improving the growing technology of grey poplar is a timely topic. In this paper we introduce algorithms which help estimate grey poplar tree volumes without having to use volume tables. Based on the performed evaluations, both equations can be used for single tree volume estimation with an error of less than 5%.
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Test of the Plant Density Reaction of Genotype Sunflower Hybrids
113-119Views:580In order to produce sunflower in Hungary today it is important to develop hybrid-specific cropping technologies. The ever widening number of hybrids makes the constant examination of genotypes necessary from the viewpoint of genotype-environment interactions and critical elements. Plant density as a complex factor puts strain on the pathological features, yield and quality of sunflower. The experiment’s main objective is to find the optimal plant density for both the genotype and external factors.
As a result it can be stated that the optimal crop density is between 45,000-75,000 plant/ha. In 2001 the optimal density was 55,000 plant/ha. The Aréna PR and the Alexandra PR hybrids produced the greatest yields (3511 kgha-1; 3338 kgha-1). In the growing season of 2002, the yields were higher than in the previous year and the optimal crop density was 45,000-65,000 plant/ha. The best yields were produced by the Aréna PR and Alexandra PR hybrids in this year again (4102 kgha-1; 4267 kgha-1) and in 2003, 45,000-65,000 plant/ha proved to be the best crop density. The highest yield was produced by the Alexandra PR.
Analyzing the growing seasons of 2001, 2002 and 2003 it can be declared that as a result of dry climate of the three years yields were higher. It can be stated that the yield is decreased by higher than average of precipitation in the growing season. -
Micropropagation of Leuce-poplar clones and its role in selection breeding
43-48Views:389Leuce-poplars (mostly white poplar and its natural hybrid grey poplar) are native tree species trough Hungary. They are covering more than 4.0 per cent of the Hungarian forested area (77 000 ha). The white (grey) poplars play a significant role in the forestation under sandy soil site conditions as well as they are of importance from nature conservation point of view as well.Long-term selection breeding work is going on at the National Agricultural Research and Innovation Centre Forest Research Institute (NARIC FRI), involving selection of fast-growing Leuce-poplar clones under dry site conditions.Micropropagation technology is relatively quickly spreading in forestry. In vitro multiplication of trees is applied mainly in fruit growing in Hungary, in case of forestry it is used mainly for selection breeding.This paper presents a short overview on the micropropagation trials with different Leuce-poplar clones and the early evaluation of the seedlings growing of the micropropagated clones/varieties. -
Genetic progress in winter wheat quality and quantity parameters
71-75Views:462Wheat production is significant branch of Hungarian crop production (with about 1 million hectares of sowing area). Weather anomalies resulted by climate change have increased the importance of biological basis in wheat production. Yield quality and quantity parameters of three wheat genotypes sown on chernozem soil type after maize pre-crop were studied in a long-term field experiment. Yield amount of the studied genotypes varied between 2894 and 8074 kg ha-1 in 2017 and between 5795 and 9547 kg ha-1 in 2018 depending on the applied treatments. Based on our results it can be stated that in both studied crop years the highest yield increment was realized by the application of the nutrient supply level of N30+PK. As the result of the application of the optimum mineral fertilizer level – in contrast to the control – resulted in significant yield increment in both crop years. The results of the long-term field experiment prove that water utilization of the studied wheat varieties / hybrids was improved by the application of the optimal nutrient supply. Furthermore, the water utilization of the latest genotypes was more favorable by both the control and the optimum nutrient supply level treatments. Analyzing the quality parameters of winter wheat using the NIR method it has been stated that the quality results of the well-known genotype (GK Öthalom) were better than those of the new genotypes. A negative correlation between winter wheat quality and quantity parameters has also been confirmed. As the result of the mineral fertilizer application protein and gluten content of winter wheat increased to a significant extent.
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Comparative analysis on the fertiliser responses of Martonvásár maize hybrids in long-term experiments
111-117Views:244The results of experiments carried out in the Agricultural Research Institute of the Hungarian Academy of Sciences clearly show that in the case of hybrids grown in a monoculture greater fertiliser responses can be achieved with increasing rates of N fertiliser than in crop rotations. In the monoculture experiment the parameters investigated reached their maximum values at a rate of 240 kg/ha N fertiliser, with the exception of 1000-kernel mass and starch content. In both cases the starch content was highest in the untreated control, gradually declining as the N rates increased. Among the parameters recorded in the crop rotation, the values of the dry grain yield, the 1000-kernel mass, the protein yield and the starch yield were greatest at the 160 kg/ha N fertiliser rate, exhibiting a decrease at 240 kg/ha. Maximum values for the protein content and SPAD index were recorded at the highest N rate. It is important to note, however, that although the N treatments caused significant differences compared to the untreated control, the differences between the N treatments were not significant.
In the given experimental year the values achieved for the untreated control in the crop rotation could only be achieved in the monoculture experiment at a fertiliser rate of 160 kg/ha N, indicating that N fertiliser rates could be reduced using a satisfactory crop sequence, which could be beneficial from the point of view of environmental pollution, crop protection and cost reduction.
The weather in 2006 was favourable for maize production, allowing comparative analysis to be made of the genetically determined traits of the hybrids. Among the three hybrids grown in the monoculture experiment, Maraton produced the best yield, giving maximum values of the parameters tested at a fertiliser rate of 240 kg/ha N. The poorest results were recorded for Mv 277, which could be attributed to the fact that the hybrid belongs to the FAO 200 maturity group, while the other hybrids had higher FAO numbers. Maraton also gave the highest yields in the crop rotation experiment at the 160 kg/ha N level. All three hybrids were found to make excellent use of the natural nutrient content of the soil.
It was proved that the protein content of maize hybrids can only be slightly improved by N fertilisation, as this trait is genetically coded, while the starch content depends to the greatest extent on the ecological factors experienced during the growing season. -
Evaluation of dry matter accumulation of maize (Zea mays L.) hybrids
35-41Views:1066The increase of the grain yield of maize is closely correlated with its seasonal dry matter accumulation. Dry matter is accumulated into the grain yield during the grain filling period. The following maize hybrids were involved in the experiment: Armagnac FAO 490, Loupiac FAO 380 and Sushi FAO 340. In order to determine dry matter content, two samples per week were taken on the following days: 22nd, 25th, 28th, 31st August, 4th, 7th, 14th, 18th, 22nd, 25th, 29th September and 2nd, 6th, 9th, 13th October. In the course of sampling the weight of 100 grains from the middle section of 4 ears was measured in 4 replications. Dry matter content was determined after drying to constant weight in a drying cabinet at 60 °C. Harvesting was performed on 13th October 2017.
The daily precipitation sum was determined by local measurements, while the daily radiation and temperature data were provided by the Meteorological Observatory Debrecen of the National Meteorological Service in Budapest. Among the agrometeorological parameters, an analysis was made of the precipitation during the growing season, effective heat sums during the vegetative and generative phase, and the water supplies. The daily heat sums were determined using the algorithm proposed.
The amount of precipitation in the winter period before the 2017 growing season was 210 mm. The soil was saturated until its field capacity. The rather dry and warm March and April had a favourable effect, but there was no worthy amount of precipitation until May (51 mm) due to the condition of the dried seedbed. Sowing was performed on the 5th of May 2017 in a randomised small plot experiment. There was favourable precipitation and temperature during the growing season, thereby providing ideal conditions for maize development, growth and yield formation. There was near average amount of precipitation in each year. The total amount of precipitation in the summer period is 342 mm. Temperature was mostly above the average, but there was no long and extremely warm period.
The Armagnac hybrid reached its highest dry matter mass 126 days after emergence. Physiological maturity was reached sooner (on the 119th day) in the case of Loupiac, and even sooner in the case of Sushi (116th day). The thousand grain weight of Sushi (which has the shortest ripening period) was 286 g at the time of physiological maturity, while that of Loupiac was 311 g. Compared to Sushi, Armagnac showed 12 g more dry matter accumulation (306 g). In the case of all three examined hybrids, physiological maturity was preceded by an intensive phase, when the dynamics of dry matter accumulation was rather quick. On average, Sushi gained 2.8 g dry matter per day between 103 days following emergence and physiological maturity, while the same values were 3.2 g for Armagnac and 3.3 g for Loupiac. The aim of the regression line slope is to predict the behavior of the dependent variable with the knowledge of the values and characteristics of the independent variables using the regression line equation. Furthermore, to determine how the location affected the dynamic of dry matter accumulation in the Armagnac, Loupiac and Sushi hybrids. In regression analysis, the coefficient of explanation showed that the effect of day in the Armagnac was 97%, in the Loupiac 94%, in the Sushi 90 %. The determination coefficient (R2) is useful in determing how the regression equation fits. But, as we have seen, the determination coefficient alone is not sufficient to verify the model’s accuracy, in addition to the determination coefficient (R2), the normality of the data or the residuals, the variance of the variables at different levels, the independence of the data relative to time and non-oblique. Observations are evaluated for the correctness of the fitted model.
Dry matter values decreased evenly and slightly following physiological maturity. According to our research results, it was established that physiological maturity is followed by a moderate dry matter loss. Until harvesting, Armagnac lost 40 g of its thousand mass weight in 29 days, while the same value pairs were 69 g in 36 days for Loupiac and 29 g in 39 days for Sushi. Loupiac – which had the highest weight at the time of physiological maturity – lost the most of its dry weight; therefore, Armagnac and Sushi had higher values at the time of harvesting.
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Determining elements of variety-specific maize production technology
157-161Views:324Our aim was to work out such new maize fertilizer methods and models which can reduce the harmful effects of fertilization, can
maintain the soil fertility and can moderate the yield fluctuation (nowadays 50-60 %).
The soil of our experimental projects was meadow soil. The soil could be characterized by high clay content and pour phosphorus and
medium potassium contents. In the last decade, out of ten years six years were dry and hot in our region. So the importance of crop-rotation
is increasing and we have to strive for using the appropriate crop rotation.
The yields of maize in monoculture crop rotation decreased by 1-3 t ha-1 in each dry year during the experiment (1983, 1990, 1992,
1993, 1994, 1995, 1998, 2000, 2003, and 2007). The most favourable forecrop of maize was wheat, medium was the biculture crop rotation
and the worst crop rotation was the monoculture.
There is a strong correlation between the sowing time and the yield of maize hybrids, but this interactive effect can be modified by the
amount and distribution of precipitation in the vegetation period. At the early sowing time, the grain moistures were 5-12 % lower compared
to the late sowing time and 4-5 % lower compared to the optimum sowing treatment.
There are great differences among the plant density of different maize hybrids. There are hybrids sensitive to higher plant density and
there are hybrids with wide and narrow optimum plant densities.
The agro-ecological optimum fertilizer dosage of hybrids with a longer season (FAO 400-500) was N 30-40 kg ha-1 higher in favourable
years as compared to early hybrids.
We can summarize our results by saying that we have to use hybrid-specific technologies in maize production. In the future, we have to
increase the level of inputs and have to apply the best appropriate hybrids and with respect to the agroecologial conditions, we can better
utilize the genetic yield potential. -
The effect of season and fertilizer on the LAI, the photosynthesis and the yield of the maize hybrids with different genetic characteristics
27-34Views:394The experiment was carried out in Debrecen, at the Experimental Station of the University of Debrecen Centre of Agricultural Sciences, Department of Crop Production and Applied Ecology. We tested 10 various hybrids with their own genetic characteristics for five different fertilizer doses, in addition to the parcels without fertilization. The three factors of production technology jointly determine the successfully of maize production, but in different measure. The yield and the stability of yield of maize can be increased with hybrid-specific technologies.
In 2004-2005 experiment years the favorable results reached were due to the rainy season. There were significant difference between the productivity of maize hybrids. The N 40, P2O5 25, K2O 30 kg/ha treatment caused the highest increase of yield (3-5 t/ha) compared to the control (parcels without fertilization). The reaction of hybrids to the further fertilizer doses was different. The agro-ecological optimum of NPK fertilization was N 120, P 75, K 90 kg of the most hybrids.
During the experiment, we tested the moisture loss of the five hybrids. The seed moisture content at harvest was higher than in previous years due to the rainy seasons. The seed moisture content of harvest of FAO 200-300 hybrids were about 20%. It changed between 21-24% in the case of hybrids with longer vegetation period (FAO 400), the seed moisture content of Mv Vilma (FAO 510) was 24.21-25.04% in the average of fertilizer treatments. There is an important difference between the moisture loss ability of hybrids which changed 0.2-0.6%/day. The moisture loss of hybrids changed depending on the fertilizer treatment; usually, it was more favorable in the optimal fertilizer dose (N120+PK).
In the case of tested hybrids, we measured the highest LAI and photosynthetic activity at the optimal treatment, N 120, P2O5 75, K2O 90 kg/ha in the respect of efficiency and environmental protection, and the yield was high also for this treatment. There are significant difference between the LAI, the photosynthetic activity and the yield of hybrids, and these values could change depending on the treatment of fertilization. -
Development of an InDel marker set to establish hybridization between wild boar and domestic pig (Sus scrofa) breeds
21-25Views:639Wild boar and domestic pig breeds belong to the same species (Sus scrofa), so they can easily have viable offspring. This could be a problem in preserving the genetic lines of wild boars, keeping clean the food industry from lower-grade hybrid boar meat, and „producing” ethically questionable trophies, too. The aim of our study was to develop a cost-efficient, fast, easy and accurate marker set which can separate the wild boars from hybrids and domestic pig breeds.
The InDel markers were developed using 59 full pig genomes of 17 different breeds (e.g. Duroc, Large White, Landrace, Mangalica, wild boar). Sequence differences between the genomes of wild boars and domestic breeds were identified in variant call files, and verified using the IGV software. Wild boar, mangalica and duroc specific primers to amplify the chosen InDel regions were designed using Primer3.
After preliminary tests five markers were chosen, three wild boar specific, one Mangalica specific and one Duroc specific one. Fluorescently labelled primers were used to make the valuation easier and more accurate with capillary electrophoresis instead of gel-electrophoresis. The markers were optimised individually and in multiplex conditions and tested in samples of 11 breeds.
In conclusion, a new, faster and cheaper set was developed to separate the wild boars from the hybrids and domestic breeds. Based on the preliminary testing on wild boars, duroc and mangalica breeds zero samples resulted false negative, so it is 100% accurate. In addition, it is a much more cost- and time-effective way than testing every single sample with STR sets.
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Production of corn in Serbia in the light of climate change
305-322Views:356This paper presents the basic elements of maize production technology in Serbia. For their good knowledge, it is necessary to be familiar with the basic biological requirements of the species, phenology and its particular requirements for water, heat, soil conditions and mineral nutrition. A special emphasis in this paper is given to recent research by the author regarding the correction of production technology (selection of hybrids, time and density of sowing, etc.) from the aspect of knowing the weather conditions of the year to the moment of sowing and the amount and position of available nitrogen in the soil profile during the spring before sowing. In the light of predicted climate change conditions for the Republic of Serbia, the authors of this paper have proposed and appropriated adaptation measures. The general conclusion is that there are no general recipes for the correct production technology of this plant species, but should be adapted to each specific year, field and hybrid.
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The Effect of Tillage Treatments on Soil Temperature at Planting and on Corn (Zea mays L.) Yield
40-44Views:550The effect of soil temperature was evaluated on the yield of the Occitan corn hybrid at a depth of 5 cm. We examined this effect on the time required from planting to emergence for three average durations: five, ten and fifteen days, all calculated from the day of planting. Winter plowing (27 cm), spring plowing (23 cm), disc-till (12 cm) treatments and 120 kg N per hectare fertilizer were applied. As a result of our analysis, we determined the post planting optimum soil temperatures for various time periods. The average soil temperature for a time period of 15 days post planting is the most usable for determining actual yields, followed by ten days, with five days proved to be the least usable (winter plow R2 = 0.86, spring plow R2 = 0.87, disc-till R2 = 0.64).
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Impact of tillage systems on maize emergence
129-136Views:473In Europe, there has been a significant change in the way tillage is approached in recent years. This change is due to a growing awareness among farmers, politicians and society as a whole that soil is not a renewable resource in itself. From an agricultural point of view, the greatest impact on soil condition can be achieved through the use of the applied tillage systems. My research takes this approach as a basis when examining the different tillage systems and their impact on the environment. In this context, conventional and a variety of no-tillage systems are examined in this paper. As a next step, it is examined how the environmental conditions created by the different tillage systems influence the emergence of maize hybrids. The analyses are carried out in a multi-factorial, long-term tillage field experiment. The same batch of the same hybrid seed was sown in several crop years, and the effects of environmental conditions on the emergence process were examined. Environmental effects and emergence-related uptake were measured in the examined plots. Measurements of environmental effects included air temperature, precipitation, soil temperature measured at seeding depth, as well as % cover of stem residue on the surface in the treated plots. The first emergence time measurements of the sown crop in the plots of each treatment were compared and relationships between these factors were investigated.
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Genetic diversity study of Hungarian honey bee (Apis mellifera L.) colonies – Previous announcement
61-64Views:668In Hungary, the Apismelliferacarnica is the native breed which is the only recognised and breedable honey bee in the country. It is assumed that there are a number of non-native and hybrid honey bee breeds in Hungary. The microsatellite and mitochondrial DNA surveys applied here will be utilised to easily and accurately identify the various sprads, and open up new ways in the research of honey bees. The isolation of the genomial DNS from 5 to 7 day old larvae samples was successfully carried out. In the future the plan is to carry out the measure with microsatellite markers. As an initial step the optimal annealing temperature was identified. In the mitochondrial DNA survey the COI-COII mytochonrial regional primer due to its low anneling temperature cannot be used with any normal sequencing methods. By using these method the aim of this research is the measurement of genetic variance.
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Study of plant physiological parameters in winter oilseed rape (Brassica napus var. napus f. biennis L.) production on chernozem soil
111-115Views:604We made plant physiology examinations in Arkaso winter oilseed rape hybrid substance: relative chlorophyll content (SPAD) and leaf area index (LAI) measurements. The experiment was set in University of Debrecen Agricultural Sciences Center at Látóképi Experimental Station in four replications, in two different sowing times (I. sowing date on 08/22/2014 and II. sowing date on 09/09/2014 sowing againhappened because of the incomplete germination in the second subtance 01/10/2014) Three different plant density 200, 350 and 500 thousand ha-1, under the same nutrient supply, 45 cm row spacing. The experiment was green crop of winter wheat. The relative chlorophyll content (SPAD) and leaf area index (LAI) measurements were made in seven different times. We measured the maximum value of chlorophyll content in the first sowing time at 500, and the second sowing time at 350 thousand ha-1 plant density. The measurement results proved that there was a linear relationship between the number of plants and the LAI. The maximum leaf area index values we measured in both the sowing time at 500 thousand ha-1 reached.
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Investigation of combining ability and superiority percentages for yield and some related traits in yellow maize using line × tester analysis
5-14Views:417Combining ability estimation is an important genetic attribute for maize breeders in anticipating improvement in productivity via hybridization and selection. This research was carried out to investigate the genetic structure of the 27 F1 maize hybrids established from nine lines derived from Maize Research Department and three testers, to determine general combining ability (GCA), determine crosses showing specific combining ability (SCA) and superiority percentages for crosses. Nine lines, three testers, 27 F1 hybrids and two check commercial hybrids (SC162 and SC168) were studied in randomized complete block Design (RCBD) with three replications during 2016. The results of mean squares showed that significant and highly significant for most studied traits (days to 50% tasseling, days to 50% silking, plant and ear height, ear position, ear length, no. of kernels per row, 100-kernel weight and Grain yield). Estimates of variance due to GCA and SCA and their ratio revealed predominantly non-additive gene effects for all studied traits. Lines with the best GCA effects were: P2 (line 11) and P6 (line 21) for grain yield, for testers Gm174 and Gm1021 had significant GCA effects for grain yield. The hybrids P5×Gm1021, P6×Gm1021, P7×Gm1021, P8×Gm1002, P9×Gm1002 had significant and negative SCA effects for grain yield. Crosses P1×Gm174, P2×Gm1002, P5×Gm1021, P6×Gm174, P6×Gm1021, P7×Gm1021, P8×Gm1002, P9×Gm1021 were the best combinations manifested and significant superiority percentages over than check varieties (SC162 and SC168) for most studied traits. Therefore, these hybrids may be preferred for hybrid crop development.
Abbreviations: GCA general combining ability; SCA specific combining ability
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Evalution of energy for bioethanol production
77-80Views:422The objective of this study was the ethanol which classified as agro fuels. The aim of our research was the calculation of efficiency of bioethanol production, and evaluates the yield of maize hybrids grown for this purpose. We examined the energy demand of corn production per hectare in two vintages of 2009 and 2010. The focus of the experiment was placed in three different doze of fertilizer. Results show that the control corn plot used the least amount of non-renewable energy. Improving starch yield by adding fertilizer required additional nonrenewable energy inputs. So then the invested energy has a great impact on the efficiency.
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Andrological examination of Hungarian Large White and Landrace boars
5-10Views:394The Hungarian Large White and Hungarian Landrace pig breeds have outstanding lifetime performance, production parameters and crossbreeding ability. Nevertheless, during the recent decades, these breeds could not compete with the West European hybrid pigs concerning on production results. In this study, we made a complex andrological evaluation of boars in different Hungarian nucleus breeding stocks. After taking blood and semen samples, performing gonadotropin-releasing hormone (GnRH) challenge, ultrasonic and thermographic examinations were done. Laboratory tests were accomplished in reproduction labs of NARIC ABNMS. Our goal was to determine the reproductive performances of boars and collect samples for future genetic examinations, respectively.