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Development of seed analyses by means of various matrix solutions and the MALDI-TOF MS technique
53-57Views:273The earth's population is growing steadily, currently accounting for about 7.3 billion people. Population growth causes food demand to rise, approximately 36 million people die each year due to starvation or related diseases. One solution to this problem is the continuous examination and development of the agricultural economy. In this study, matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (MALDI -TOF MS) were used to analyse of sunflower, soybean and hemp. In order to analyse the protein of maize, this method has already been applied. However, for sunflower, soy and hemp, it is necessary to develop a sample preparation method. Choosing the optimal matrix solution for ionization the traget molecule is an essential part of developing the method. Our aim is to compare two different matrix solutions (α-HCCA, SA matrix), based on the properties (intensity, noise ratio, value of spectra) of the spectra.
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The importance of selenium in the living world and in the scientific research
278-286Views:137Presently, selenium (Se) is one of the most investigated microelements. It has an important proven role in many vital processes. Directly or indirectly, selenium deficiency can play a role in the development of many diseases. On the other hand, the concentration range in which selenium is essential is narrow; there is a narrow gap between necessary and toxic content in dietary intake. In this context, selenium contamination poses a further health risk for people if they live near the industrial areas and mining activity.
In this paper, we comprehensively introduce the very important trace element selenium. We studied the base parameters, deposit, analytic and deficiencies, problem of contamination and also the solution of contamination problems of selenium. -
Sunflower in Romanian agriculture
153-163Views:192Romania is one of the largest producers of sunflower closely followed by Bulgaria, Hungary, France and Spain. The total sunflower production increased after 1990, due the increase of cultivated surface during the moderate yield average increasing rate. Last year, Romania was ranked first in the European Union (EU) for the area cultivated with sunflower and the total production of sunflower seeds, according to the National Statistics Institute (INS). Romania's position on the map of sunflower producers area is supported by about 1 million hectares sown. In the segment of sunflower productivity, Romania has an unfavourable position, situated in the last places in the classification of the EU's most productive agricultures. In order to increase production per hectare, it is required to perform measures aimed at the elements of sustainable cultivation technology, including the stability of the production level by ensuring phytosanitary protection.
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Studies of Expression of Peptaibol Synthetase of Trichoderma reesei
188-190Views:117Because of the potential importance of peptaibols in the biological control of plant diseases, a transgenic, a T. reesei strain carrying a tex1-promoter: goxA fusion plasmid was constructed for furthur studies. The peptaibol synthetase gene (which is highly similar to T. virens tex1) was identified in the genome sequence of T. reesei. A 900 bp 5’ upstream noncoding fragment, presumed to include the promoter region of tex1, was cloned into the pSJ3 plasmid (which contains the Aspergillus niger goxA gene encoding glucose oxidase). Finally, we transformed T. reesei with the tex1-promoter: goxA fusion containing pSJ3 plasmid.
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The effect of water-stress on the mineral nutrition of fruit plantations
187-192Views:84Besides agro-techniques the climatic conditions play an important role in agricultural production. Weather extremes are
significant hazards to many horticultural regions all over the word. It has a profound influence on the growth, development and yields of a
crop, incidence of pests and diseases, water needs and fertilizer requirements in terms of differences in nutrient mobilization due to water
stresses. Nowadays, the weather extremes cause more and more problems and significant hazards to many horticultural regions in Hungary.
The aim of this study is to explore the problems of nutrient uptake followed from climatic anomalies and response it. In this study
we focus on water supply problems (water-stress).
Reviewing the effects and nutrient disorders caused by climatic anomalies, the following statements can be taken:
· Nutrient demand of trees can be supplied only under even worse conditions.
· The most effective weapon against damage of climatic anomalies is preventative action.
· Proper choice of cultivars, species and cultivation should provide further possibilities to avoid and moderate the effects of
climatic anomalies.
· Fruit growing technologies especially nutrition should be corrected and adjusted to the climatic events as modifier factors.
· The role of foliar spraying, mulching and fertigation/irrigation is increasing continuously.
· Urgent task of the near future is to correct and adjust the tested technologies of fruit growing according to these climatic events as
modifier factors.
Optimal nutrient supply of trees decreases the sensitivity for unexpected climatic events. To solve these problems supplementary, foliar
fertilization is recommended, which adjusted to phonological phases of trees. Moreover, mulching is regarded as an excellent water saving
method. -
Reduction of ragweed pollution in industrial utilization system
69-72Views:67In Hungary 6.2 billion hectares are used for agricultural and from this area 5.0 billion are polluted by ragweed. In addition, the export of agricultural products will be threatened because of their pollution. Ragweed results also problems in human medicine because of its pollen allergy. More than 30% of the habitants are affected directly or indirect by allergyc diseases. On an annual basis nearly 60 billion HUF is spent on defense against ragweed. From this budget 30 billion HUF comes from the damage of the agriculture and other 30 billion for the cost of the therapy and health insurance. To solve the problems caused by ragweed needs new ideas. The proposals are the gathering and comprehensive utilization. A leap forward, from the ragweed new, market–orientated product should be developed.
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The Carpathian lingonberry, raspberry and blackberry fruit extracts feature variable antimicrobial efficiency
27-32Views:313Wild berry is an excellent source of phytonutrients and/or bioactive compounds associated with significant therapeutic properties, so that they have been utilized in folk medicine and traditional nutrition throughout centuries. Multiple health-promoting effects, such as anti-inflammatory, anti-diabetic, anti-heart and coronary disease properties were attributed to such wild berries. It has also been proved that berries could feature antimicrobial effects that could be of a great importance for the prevention of food-feed poisoning and fighting back antibiotic resistance.
In this study, we investigated the antimicrobial properties of lingonberry (Vaccinium vitis-idaea), raspberry (Rubus idaeus) and blackberry (Rubus fruticosus) crude and ethanolic extracts prepared from fruits obtained from the spontaneous flora of Eastern Carpathian Mountains situated in Transylvania. The antimicrobial effect of crude and alcoholic extracts were assessed on four Gram-negative, five Gram-positive bacteria and one yeast species using the agar diffusion method. The studied bacteria can cause food or feed spoilage and foodborne diseases. Our results indicate the significant inhibitory effect of lingonberry extracts in the case of Gram-negative bacteria like Proteus vulgaris and Salmonella Hartford, while among Gram-positive bacteria the strongest inhibitory effect was observed for Bacillus species like B. cereus, B. subtilis, B. mojavensis and Micrococcus luteus. The raspberry and blackberry extracts featured milder inhibitory effects in the case of the studied bacteria species. Furthermore, we have studied the crude or ethanolic extract combinations associated antimicrobial effects synergistic/additive or antagonistic properties. Interestingly, the triple and double ethanolic extract mixes had stronger antimicrobial properties, whereas the crude extract mixes showed relatively reduced effects, if any. Our results indicate that the antimicrobial activity of studied fruit extracts obtained from wild berries can vary upon the applied extraction method and their combination formulae, so that all these considerations must be taken into account when such fruit extracts are considered for foodstuff development.
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The relationship between silver crucian carp (Carassius auratus gibelio bloch) and fish poulations of natural waters
30-36Views:89The fisheries management on inland waters is very complicated. Often the unclear propriety rights make the normal management difficult, and other times the stocking of the waters is problematic. In the last hundred years a lot of new fish species have been introduced to Hungary. Lot of them have found their place in the Hungarian fishfauna, till others have caused big problems for the fish farmers, and the anglers too. The biggest problem was caused by the topmouth gudgeon (Pseudorasbora parva Schlegel, 1842) brown bullhead (Ictalurus nebulosus LeSueur, 1819) and the silver crucian carp (Carassius auratus gibelio Bloch, 1782). In this article I show what kind of problems were caused by the silver crucian carp, because it seems that this fish is the most harmful.The damage caused by silver crucian carpposesa complex question. To get into the ponds and rivers, the crucian carps occupy the territory form the native fishspeciesandthesilvercrucian carp competes with these fishes for food, too.The silver crucian carphasa special reproduction method and because of this the speciesis expand rapidly. The silver crucian carp is able to reproduce with other cyprinids, and because the female silver crucian carps are triploids every fingerlings will be silver crucian carp.This fish is a vectorfora lot of diseases, which can easily spread over for other species.
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Comparative study of special honey products and herbhoneys
117-120Views:320Honey has a positive effect on human body due to its high content of biologically active substances (e.g. monosaccharides, vitamins, enzymes, amino acids, polyphenolic compounds). The properties of honey depend on its botanical origin due to the bioactive plant components, mainly secondary metabolites that are included in honey made by bees from nectar. Herbally infused honeys are delicious products that combine the therapeutic action of herbs and honey. Additionally, herbs can provide nutrient fortified syrup for honey bees and protect them against diseases and other ecological threats.The aim of this study was to define the physicochemical properties of multifloral honey, herb enriched natural honey and herbhoney samples. We measured the moisture content, pH value, electrical conductivity and proline content. Although great diversity was observed in the basic properties of the examined products. In our study, we found that the electrical conductivity shows the significant differ between the groups. All the samples, including the herbhoneys passes the quality standards of honeys. -
The impact of applying foliar fertilizers on the health condition of maize
105-109Views:158The nutrient supply of plants is becoming more important in plant protection since the appropriate condition of plants can be ensured by optimal and satisfactory nutrient supply to avoid nutritional deficiency diseases. Due to the extreme weather conditions, plants are forced to face several stress factors, which leads to deterioration of the plant health. The increasing occurrence of droughts poses threat to nutrient uptake through the roots since all the nutrients can be accessible to the plants only if they are in dissolved form in the soil – which is not possible in the absence of water. Therefore, the importance of foliar fertilizer is becoming a more and more significant part of the nutrient supply, because with the help of this technology the development of any nutrient deficiency can be prevented.
In this experiment, we focused on the efficiency of two different foliar fertilizers on maize. Foliar fertilizers were applied two times, once in the stage with 8 leaves and tasseling phenophase of the maize. To verify the efficiency of the foliar fertilizers, the chlorophyll content of untreated and treated plant’s leaves was measured after each application. Moreover, the length and diameter of maize cobs, thousand kernel weight, protein, oil and starch content were also measured, and the results were compared to the untreated (control) ones. According to the results, in all aspects significant differences were observed and due to the laboratory analysis of leaves, in the case of magnesium and zinc supply the foliar fertilizers were able to prevent the development of nutrient deficiency.
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Q-PCR analysis of the resistance of Hungarian Botrytis cinerea isolates toward azoxystrobin
41-44Views:76The genes being in the mitochondrial DNA primarily encode the enzymes of cellular respiration. Fungicides belonging to the family of quinol oxidase inhibitors (QoIs) play on important role in the protection against several plant diseases caused by fungi. These fungicides bind to the cytochrome bc1 complex so they block electron transport between cytochrome b and cytochrome c1. This way these fungicides inhibit the ATP synthesis consequently they inhibit the mitochondrial respiration. The QoI resistance has two mechanisms. One of them is the point mutation of the cytochrome b gene (CYTB), e.g. the substitution of a single glycine by alanine at position 143 results in high-resistance. The other is the cyanide-resistant alternative respiration sustained by the alternative oxidase.
In a cell there are several mitochondria. The phenomenon when the genomes of all mitochondria in the cell are identical is called homoplazmy. If in the cell there is wild and mutant mitochondrial DNA this is called heteroplasmy. Whether the mutation in the mitochondria causes fenotypical diversity or does not depend on the dose, i.e. it depends on the percentage of the changed mitochondrials. During our work we investigated Botrytis cinerea single spore isolates which have been collected in 2008-2009 on different host plants. Our goal was to decide whether heteroplasmy influences the level of resistance. We managed to detect the change of the level of heteroplasmy, so the change the level of the resistance due to the treatment with fungicide. -
Arrival of invasive pests in our direct environment and control opportunities
23-26Views:233The author refers about the nowadays applied practical plant protection activities against pests occuring on trees, shrubs and turfs of public domains. It is overviewed those insects, diseases and weeds which cause damages and touched also upon disturbing the local residents only and tasks to the plant protection engineer expert to manage them. Among the special features of public domains should be mentioned that the rules are difficult to harmonize according to the legal, public health, and horticulture requirements at the same time. The anti-pesticide attitude of EU and the modest range of pesticides which are applicable on public domains make difficulties in optimal management work. The author draws up proposals how to manage the complex plant protection on public domains.
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Changes of relative chlorophyll content at maize smut inoculated hybrids
55-58Views:223The leaf chlorophyll content analysis is important for several reasons. The natural or anthropogenic stressors directly effect on the chlorophyll content. Through the measurement of the chlorophyll content it is possible to obtain data concerning the physiological status of the plant, moreover the chlorophyll content is closely related to the nitrogen content, so it is linked to photosynthesis and the photosynthetic activity which determine biomass production.
One of the most common symptoms of plant diseases is the larger and smaller interveinal chlorotic areas. These might be local, or expand to the whole plant. There are multiply reasons of chlorosis such as reduction of chlorophyll content, unfavorable effects on the chlorophyll content,disorders regarding function of chloroplasts or ultimately destruction of the chloroplasts. Although such a chlorotic deviancy can contribute to significant losses in photosynthesis; however the underperformance photosynthesis of the sick plants is a more complex process.
As we unambiguously experienced during our investigations on common smut that the infected maize plants most common accompanying symptom was chlorosis on the leaves, so it is especially important to examine how the infection influenced on the chlorophyll content of different hybrids. -
The effect of fertilization and plant protection in sunflower (Helianthus annuus L.) production
57-63Views:221Sunflower is the most important oil crop in Europe which is grown on the biggest area of all the oil crops. The area of producing sunflowers in Hungary was changing to 524–704 thousand hectares in the past decade in comparison with approximately 100 thousand hectares in the 1970’s. In our experiment different sunflower hybrids were examined. The doses of fertilizers were the following in 2017 and 2018: 0–30–90–150 kg ha-1 N, 0-50-90-90 kg ha-1 P2O5 and 0–70–110–110 kg ha-1 K2O. Three different treatments of plant protection were used in the experiment to protect them from fungal infections. A rise in the dosage of nitrogen resulted in increasing infection. The biggest fungal infection was identified in 150 kg ha-1 N, 90 kg ha-1 P2O5 and 110 kg ha-1 K2O treatment. The largest infection was measured on plots without plant protection at the highest N doses, both in leaf and disc diseases. We measured the highest 41.25% in 2017 and 53.1% in 2018 Diaporthe helianthi infection, and 24.5% in 2017 and 25.5 % in 2018 Sclerotinia sclerotiorum disc infection in these plots. kg ha-1
Average yield was changing between 2.96–4.67 t ha-1 in 2017/2018. The lowest yield was obtained in the absolute control plot (without plant protection and without nutrient) in both years, which was 2.96 t ha-1 in 2017 and 3.14 t ha-1. The yields increased due to growing nutrient supply and excellent plant protection. The highest yields were 3.67 t ha-1 in 2017 and 4.67 t ha-1 in 2018 (150 kg ha-1 N, 90 kg ha-1 P2O5 and 110 kg K2O active substance). Purpose of the experiment at analysing the impact of different treatments on the profitability of sunflower production.
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The bioactive compounds of sour cherry (Prunus cerasus) with special regards to antioxidant activity and antioxidant density
83-87Views:165Evidence suggests that a diet high in fruits and vegetables may reduce the risk of chronic diseases, such as cardiovascular disease and cancer, and phytochemicals including phenolics, flavonoids and carotenoids from fruits and vegetables may play a key role in reducing chronic disease risk. Recent research has proven that sour cherry (Prunus cerasus L.) is a valuable natural source of some bioactive compounds important in human health preservation.
In our work, we identified the total antioxidant capacity and ”antioxidant density” of sour cherry varieties named ”Újfehértói fürtös”, ”Debreceni bõtermõ”, ”Kántorjánosi” and ”Érdi bõtermõ” and those of the ”Bosnyák” sour cherry clones. ”Antioxidant density” is a biological value indicator obtained in a synthetic way, which indicates the antioxidant capacity of the particular food, e. g. fruit and vegetable, per 1 Calorie. -
Differences in content of beet root and beet root juice, and it’s suitable to develop functional foods from animal sources
53-57Views:232In Western medicine is beginning to increasingly focus on the key role of healthy nutrition and lifestyle in preventing certain diseases. Therefore, it is important to emphasize the importance of primary prevention in our country, and to promote it; not only the health care team work, but broaden the recommended foods, which have benefit for human health as well.
Polyphenols are antioxidants, which are easily consumable with diet, however, food processing can influence the level of them.
In our study, nutritional assessment was made of raw beet root and a juice, made from this beet root. We investigated in which special diet referenced to eat this foods and whether this is suitable to develop functional foods from animal sources as a row material.
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Grain yield and quality of maize hybrids in different FAO maturity groups
126-131Views:64An improvement in the quality of maize grain by increasing the level of components responsible for its biological value is possible
by using genetic means. However, a change in the genotype, together with improving the nutrient properties of the grain, also has some
adverse consequences connected with a fall in yield and in resistance to diseases.
Field experiments were conducted during three years (2003, 2004and 2005) to evaluate environmental effects on grain yield and
quality responses of maize hybrids. Twenty one hybrids of various maturity groups (FAO 150-400) were planted to achieve an optimum
(60-70 000 plants per hectare) plant populations and grown under the medium-N (80 kg N ha-1) fertilization. Environmental conditions
significantly affected maize hybrid responses for grain yield, starch, oil and protein contents, and consequently, starch, oil and protein
yields per hectare. Hybrids of flint type, which have a short vegetation period, had high protein and oil content but the yield averages
were low due to the slower rate of starch incorporation. Hybrids of the dent type have a longer growing season and more intense
carbohydrate accumulation, but low protein and oil contents. In wet years there was a higher rate of starch accumulation, while dry
years are favorable for protein and oil accumulation. Positive correlation existed between starch content and grain yield and 1000-
weight as well as between oil content and volumetric weight among tested hybrids. Negatively correlation existed between grain oil and
starch content as well as between oil content and grain yield and 1000-weight. Thus, end-users that require high quality maize may need
to provide incentives to growers to off set the negative correlation of grain yield with oil and protein content. -
Cytochrome b diversity of Hungarian Botrytis cinerea strains
18-21Views:91In the mitochondrion of eukaryotes, cytochrome b is a component of respiratory chain complex III. Cytochrome b is encoded by the
cytochrome b (CYTB) gene located in the mitochondrial genome. The fungicidal activity of QoIs relies on their ability to inhibit mitochondrial respiration by binding at the so-called Qo site (the outer quinol-oxidation site) of the complex III. Since their introduction, QoIs (like azoxystrobin) have become essential components of plant disease control programs because of their wide-ranging efficacy against many agriculturally important fungal diseases like grey mould on various crops. QoI resistance primarily arises from a target-site-based mechanism involving mutations in the mitochondrial CYTB. As the management of grey mould is often dependent on chemicals, the rational design of control programs requires the information about the diversity of genes connected with resistance in field populations of the pathogen.
Monospore B. cinerea field isolates has been collected during 2008-2009 from different hosts in Hungary. PCR fragment length analysis
indicated the high frequency presence of type large intron in the isolates while in a few strains G143A substitution could also be detected.
These results indicated the heterogeneity of CYTB in the Hungarian B. cinerea populations, which possibly involve the heteroplasmy of this
mitochondrial gene, moreover indicates the existence op azoxystrobin resistant populations in Hungary.
This work was supported by NKFP-A2-2006/0017 grant. Erzsébet Fekete is a grantee of the János Bolyai Scholarship (BO/00519/09/8). -
Anti-Oxidants in Agriculture
195-200Views:82Today’s programmes aiming at enhancement of fruit and vegetable consumption have been intensified. In the unanimous view of experts, different health problems, such as those of the immune system, inflammations, and even certain cancerous diseases can be prevented and/or cured with regular consumption of fresh (raw) fruit and vegetables. It is well-known fact that among the biologically valuable components, antioxidant compounds – C- and E vitamins, as well as carotinoides – play an important role. In this field, Hungary can expect success in the future since it has excellent plant genetic stock. Regretfully, national data banks regarding cultivation technology, cultivation areas or varieties for fruits and vegetables and their antioxidant contents do not exist in Hungary. Nevertheless, in connection with the so-called “Hungaricums” its existence would be of urgent necessity. Such excellent Hungarian products are – among others – a lot of sour cherry varietiles, the Szeged green pepper and the Makó onion. They enjoy high priority as “Hungaricums” even in the European Union and such activities that support these kinds of products should be enhanced by intense and consequent research work, which may prove their role as functional foods. Presently’ the USA leads in the research of antioxidant compounds of sour cherry, and so far 17 of these compounds have been found partly in Hungarian varieties. Similar research on green, and ‘pritamin’ peppers have not gone so far since they were limited only for seasoning paprika. In Hungary, studies on onion and garlic have not been performed. It should be mentioned that due to the continental climate, these products may be cultivated, consumed or processed only in a limited period. Taking this into consideration, processing and conserving methods are needed which make the consumption of these fruit and vegetables as functional food possible year-round. Scientific establishment of this set of questions is of current concern, because consumption of these products could have an important role in improving the health status of the Hungarian population in the future.
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Sensitivity of maize hybrids to common smut under field artificial inoculation conditions
25-28Views:189Common smut disease of maize is one of the most frequent diseases of crop. In the last decades the importance of disease has decreased in feeding maize production, however its importance increasing again nowadays, especially at sweet maize hybrids. The aims of this work was to find hybrids possess of resistance, and to evaluate which ones are more or less susceptible under field artificial inoculation circumstances. Among feeding maizes the less susceptible hybrid was ‘P9578’, and the most susceptible ’NK Columbia’ hybrid, and differences in cob infection between them was significant (8.8%). At sweet corn hybrids the less susceptible was ’Prelude’, while the most susceptible was ’Jumbo’ with very high significant 74.6% differences.
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Element content analyses in the Institute for Food Sciences, Quality Assurance and Microbiology
203-207Views:111The role of chemical elements to ensure and promote our health is undisputed. Some of them are essential for plants, animals and human, others can cause diseases. The major source of mineral constituents is food, drinking water has a minor contribution to it, so the knowledge of elemental intake through food is crucial and needs continuous monitoring and by this way it promotes the food quality assurance and dietetics.
With the evolution of spectroscopic methods increasingly lower concentrations could be determined, so the elemental composition of a sample could be more precisely and fully described. Due to the results the gathered knowledge up to the present is supported and new observations can be done helping us to understand such complex systems as biological organisms are.
The quality of a food is determined by the full process of its production, consequently it starts with agricultural production so elemental-analysis usually cover the whole soil – plant – (animal) – food chain, by this way the „Fork-to-Farm” precept is true in elemental analysis field also.
The history of elemental analysis in the University of Debrecen, Centre for Agricultural and Applied Economic Sciences, Faculty of Agricultural and Food Sciences and Environmental Management, Institute of Food Processing, Quality Assurance and Microbiology goes back to 1980s when the so called Regional Measurement Central gave the background for research. The continuous deployment resulted in an obtain of an inductively coupled plasma atomic emission spectrometer (ICP-AES) in 1988, which extended the scope of examinations due to its excellent performance characteristics
compared to flame atom absorption (FAAS) and flame emission spectrometers (FES). The instrumental park retain up to date correlate to the developing analytical techniques due to acquiring a newer ICPAES in 1998 and an inductively coupled plasma mass spectrometer in 2004 – which sensitivity is three order of magnitude better compared to ICP-AES. The Institute supports the work with its own ICP-AES and ICP-MS since 2011. -
Comparison of major population parameters of Brown hare (Lepus europaeus, Pallas 1758) in two hunting fields of the Great Hungarian Plain
69-74Views:135Hungarian stock of game is not only part of our national treasure but also one of our domestic products and economic income. Not indifferent therefore the number and the state of health of our wildlife. Population decline of the Brown hare (Lepus europaeus, Pallas 1758) (one of our most important small game in Hungary) takes a long time. Demographic parameters of Brown hare was examined, particularly the factors affecting the decline of the species in Hungary. We took samples from typical habitats where the Brown hare could be found in relatively high density in our country. The article reports data of reproductive characteristics, diseases, parasites of Brown hare and other factors such as climatic and anthropogenic which could influence of the population dynamics. We mention sample collection and processing methods eg: population size estimates, examination of reproductive organs, the sex ratio and the age structure as well as the individual condition based on data of domestic and foreign authors and our partial results.
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Effects of bioactive plant extracts on immune-related gene expression of common carp (Cyprinus carpio)
49-56Views:246In recent years, intensive fish farming has led to an outbreak of several diseases, and the health status of fish can affect the economy of aquaculture. Since fish health and intestinal health are in correlation, it may also have an impact on immunity. Accordingly, many natural feed additives are being used to improve immune functions. In our study, carotenoids, oligosaccharides, and anthocyanins were applied at 1 m/m% in feed to investigate their effects on cytokines, such as interleukin-1β (IL-1β), interleukin-8 (IL-8), tumor necrosis factor-α (TNF-α), and interferon regulatory factor-1 (IRF-1) in spleen and mid-intestine of 6 months old carp. Gene expression analysis was carried out to examine IL-1β, IL-8, TNF-α, and IRF-1 mRNA levels in fish spleen and mid-intestine. The gene expression level of pro-inflammatory IL-1β decreased in the mid-intestine of carotenoid-fed carp compared to anthocyanin supplemented group, but the effects of the bioactive plant extracts were not observed on the examined cytokines compared to control fish.
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Sour cherry anthracnose and possibilities of the control with special regard to resident Glomerella population in sour cherry plantations of East Hungary
12-17Views:133Anthracnose is considered one of the most destructive diseases for sour cherry production due to the rapid development of the disease on fruits. Glomerella cingulata (Stoneman) Spauld. & H. Schrenk (anam.: Colletotrichum gloeosporioides (Penz.) Penz. & Sacc. in Penz.) has been the fungal pathogen responsible for anthracnose in last decades. Yield losses greater than 90% may occur under epidemic conditions. C. acutatum (J.H. Simmonds, 1968) strains were isolated of sourcherry plantations in East Hungary and this pathogen, new for Hungarian microbiont became recently dominant. Contrarily to the former species it is certainly transmitted with ants during fruit ripening. About third of strains proved to be cutinase producers that enable them to actively penetrate via cuticule, and these strains infect directly berries of blackberry, grape and tomato as well as plum and apple. Most of cutinase negative strains could also infect these fruits after mechanic injury. All strains of both species produce amylase, cellulase, lecithinase, lipase, polyfenoloxydase and protease in vitro, although the activity of these enzymes highly varied in the medium. The only C. acutatum strains produced noticeable amount of chitinase. Strains, tolerant to recently applied fungicides to control the anthracnose, could be isolated of sour cherry plantations that might be the cause of ineffectiveness of control measures in 2010. The mycofungicide containing mixture of three Trichoderma species in oil carrier could efficiently depress the development of anthracnose in ripening sour cherry.
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Examination for Susceptibility of CAE
33-36Views:97Caprine Arthritis Encephalitis (CAE) is a group of diseases in goats caused by a retrovirus, namely the Caprine Arthritis Encephalitis Virus. CAEV belongs to the lentivirus group within the retrovirus family. The CAEV is intimately associated with white blood cells, therfore any body secretions which contain white blood cells are a potential source of virus to other goats in the flock. Once goats are infected with CAEV, it remains infected for life and has to be eliminated.
Since not all goats infected with CAEV progress to disease, very important to test goats for infection using a serology test (ELISA, AGID, IDT). These serological tests demonstrate the presence of antibodies to CAEV in goat serum or detect the virus genome in the white blood cells. However, Swiss results (Dolf and Ruff, 1994) pointed out individual and species differencies in predisposition for this disease. This result was considered as a starting point to our examination. Microsatellite analysis was used in order to find whether there was any association between genotype and serological results, and to look for a marker associated with this disease. To date, altogether 135 goats have been examined. Unfortunately, a significant association between serological results and genotype was not found using the Chi2 test