No 1 (2019)
Articles

Development of seed analyses by means of various matrix solutions and the MALDI-TOF MS technique

Published May 23, 2019
Csilla Bojté
1Kerpely Kálmán Plant Production and Horticultural Sciences Doctoral School, 4032 Debrecen, Böszörményi út 138
Csaba Lovász
WESSLING Hungary Ltd, 1045 Budapest, Anonymus u. 6
Eszter Tímár
NÉBIH Central Seed Analysis Laboratory, 1024 Budapest, Kis Rókus u. 15/a
László Lajkó
PHYTOPATENT Ltd., 1012 Budapest, Mátray u. 10. 2.em/ 3.
János Nagy
University of Debrecen, Faculty of Agricultural and Food Sciences and Environmental Management, 4032 Debrecen, Böszörményi út 138.
Nikoletta Edit Nagy
Kerpely Kálmán Plant Production and Horticultural Sciences Doctoral School, 4032 Debrecen, Böszörményi út 138
Karina Bodnár
Kerpely Kálmán Plant Production and Horticultural Sciences Doctoral School, 4032 Debrecen, Böszörményi út 138
Laura Czerődiné Kempf
Szent István University, 2100 Gödöllő, Páter Károly utca
Brigitta Horváth
Livestock Farming Sciences Doctoral School, 4032 Debrecen, Böszörményi út 138.
Adrienn Micsinai
WESSLING Hungary Ltd, 1045 Budapest, Anonymus u. 6.
Szilárd Tóth
Fleischmann Rudolf Research Institute, 3356 Kompolt, Fleischmann u. 4
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How to Cite

APA

Bojté, C., Lovász, C., Tímár, E., Lajkó, L., Nagy, J., Nagy, N. E., Bodnár, K., Czerődiné Kempf, L., Horváth, B., Micsinai, A., & Tóth, S. (2019). Development of seed analyses by means of various matrix solutions and the MALDI-TOF MS technique. Acta Agraria Debreceniensis, (1), 53-57. https://doi.org/10.34101/actaagrar/1/2370

Abstract

The earth's population is growing steadily, currently accounting for about 7.3 billion people. Population growth causes food demand to rise, approximately 36 million people die each year due to starvation or related diseases. One solution to this problem is the continuous examination and development of the agricultural economy. In this study, matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (MALDI -TOF MS) were used to analyse of sunflower, soybean and hemp. In order to analyse the protein of maize, this method has already been applied. However, for sunflower, soy and hemp, it is necessary to develop a sample preparation method. Choosing the optimal matrix solution for ionization the traget molecule is an essential part of developing the method. Our aim is to compare two different matrix solutions (α-HCCA, SA matrix), based on the properties (intensity, noise ratio, value of spectra) of the spectra.

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