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Micropropagation of Leuce-poplar clones and its role in selection breeding
43-48Views:148Leuce-poplars (mostly white poplar and its natural hybrid grey poplar) are native tree species trough Hungary. They are covering more than 4.0 per cent of the Hungarian forested area (77 000 ha). The white (grey) poplars play a significant role in the forestation under sandy soil site conditions as well as they are of importance from nature conservation point of view as well.Long-term selection breeding work is going on at the National Agricultural Research and Innovation Centre Forest Research Institute (NARIC FRI), involving selection of fast-growing Leuce-poplar clones under dry site conditions.Micropropagation technology is relatively quickly spreading in forestry. In vitro multiplication of trees is applied mainly in fruit growing in Hungary, in case of forestry it is used mainly for selection breeding.This paper presents a short overview on the micropropagation trials with different Leuce-poplar clones and the early evaluation of the seedlings growing of the micropropagated clones/varieties. -
Investigation of the Resistance Against the Fusarium solani at Different Pea Varieties and Combinations of Breeding
157-160Views:32The Fusarium species are soil and polyphage parasits, and the rate of damage, caused by them, highly depend on interactions between climatic and edaphic factors and also on sensitivity of cultivars. Even though about 70-80 percent of the widely grown green peas cultivars is resistant to Fusarium oxysporum f. sp. pisi 1. race, the rate of Fusarium infections and severity of symptoms increased in the latest years. It is supposed that another Fusarium sp. the Fusarium solani has been spreading. The most exact way to study the cultivars in a provoking garden established in natural environment, where the pathogen is artificially enriched to a level, at which the cultivars can be distinguished according to their susceptibility. In the provoking garden the reaction against to Fusarium of our breeding lines and our registered cultivars and cultivars existed on the National List (including cultivars with well-known susceptibility as standards) are examined year by year. In our experiments we could found two green peas cultivars to be resistant to Fusarium solani (Early sweet (13,36%) and Lora (16,9%)) The breeding lines Margit and 8607/75-3-2 proved to be the most susceptible to Fusarium solani (94,4% and 73,1% infected plants, respectively).
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Upgrading breeding value estimation in beef cattle
451-458Views:188This paper gives a summary of the possibility for applying genomic information for breeding value estimation in beef cattle breeding. This process is called genomic prediction and is now widely used in dairy cattle globally as well as in some beef and sheep populations. The advantage of genomic prediction is a more accurate estimate of the genetic merit of an individual at a young age thereby facilitating greater annual genetic gain, predominantly through shorter generation intervals. Genomic predictions are more advantageous for sex-linked (e.g., milk yield), low heritability (e.g., fertility) and difficult-to-measure (e.g., feed intake) traits. The larger the reference population, on average, the more accurate the genomic predictions; additionally, the closer genetically the reference population is to the candidate population, the greater the accuracy of genomic predictions. Research is continuing on strategies to generate accurate genomic predictions using a reference population consisting of multiple breeds (and crossbred). Retrospective analysis of real-life data where genomic predictions have been operation for several years clearly shows a benefit of this technology.
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Random regression models for genetic evaluation of performance of the Hungarian show-jumping horse population
87-91Views:125The aim of the study was to estimate genetic parameters for show-jumping competition performance using random regression model. Show-jumping competition results collected between 1996 and 2009 were analyzed. The database contained 272 951 starts of 8020 horses. Identity number and gender of the horse, rider, competition date, the level of the competition and placing were recorded in the database. Competition levels were categorized into five groups. Weighted – competition level used – square root transformed placing was used to measure performance of horses. The random regression model included fixed effects for gender, year and place of competition, and random effects for rider, animal and permanent environment.
Later performance of show-jumping horses measured with weighted square root ranks is less influenced by rider and permanent environmental effects than performance at the beginning of a horse’s sporting career. Heritability increased continuously from 6.3 years of age (2296 age in days), values were in the range of 0.07 and 0.37. Higher heritability was found in later ages. Weak genetic and phenotypic correlation was found between the early 4–5–6 years of age and older (7, 8, 8+) age classes. From 8.5 years of age (3132 days old) there were strong genetic and phenotypic correlations between neighboring age groups. For the same age classes moderate and strong genetic and phenotypic correlation was found. Genetic correlation between 13.5 years of age and older horses was very strong.
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Comparison of Variability among Irradiated and Control Inbred Maize Lines via Morphological Descriptions and Some Quantitative Features
70-73Views:60Knowledge of genetic diversity in breeding material is fundamental for hybrid selection programs and for germplasm preservation as well. Research has been done with nine irradiated (fast neutron) and four non-treated inbred lines. The aims of this study were (1) to investigate the degree of genetic variability detected with morphological description (based on CPVO TP/2/2) in these materials, (2) to compare the genetic changes among irradiated and non-irradiated maize inbred lines (based on some quantitative features). The irradiation did not change any of the characteristics clearly in positive or negative way, which can be related to the fact that the effect of induced mutation on genetic structure cannot be controlled. From the irradiated lines we have managed to select plants with earlier ripening times and better phenotypes. We could distinguish 3 main groups by the morphological features; these results match our expectations based on pedigree data. Markers distinguishable on the phenotypic level (e.g. antocyanin colouration, length of tassels) were significant in all lines.
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A review on the trend of livestock breeds in Laos
227-237Views:306When the livestock sector is considered an essential role in farmers' livelihood, and local breeds are still the critical component of animal breeding in Laos. Thus, there is a need to review the previous, current situation and prospects for Laos' livestock production. It aimed to study the different traits among the existing dominant local and improved livestock breeds (cattle, pig, and goat) and a more in-depth study on the livestock sector's previous evolving and prospects. Our findings revealed that the general trend of livestock population in Laos increased year-by-year for over 45 years, except for 1995 to 2000. It was decreased by almost 22% due to the new national strategy on economic revolution. The first foreign breeds were introduced into Laos, i.e., Large White, Landrace, and Duroc in 1980, Red Sindhi bulls in 1998, the frozen semen of Red Brahman 2003, and Bach Thao goats between 2001 to 2003. Unfortunately, many data are still not precise for introducing other foreign breeds like Boer goats, Thai Grey and Red Brahman. There were many missing data on the improvement of livestock breeds in Laos, including the local and improved breeds' reproductive and productive performance, which needs more study and research. We conclude that the semi-complex farm operation should be applied to improve livestock breeding and their productions in Laos.
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Effect of G2548A polymorphism in the leptin gene on the BMI level in human population
5-10Views:129The polymorphism in leptin (LEP 2548A) seems to influence obesity among others genes. The aim of this study is to investigate the effect of the G2548A polymorphism on body mass index. We included 79 people from Slovakia with some genetic relatedness and used barrels kit to isolate the genomic DNA from an adenoblast swab- from the salivary. PCR products were amplified by pursued polymorphisms and G2548A, we restriction-analyzed them and then we identified the specific fragments describing the presence of chosen SNP polymorphism by the agarose electrophoresis, to analyze SNP polymorphism by PCR-RFLP method.
The LEP gene had increased frequency of G allele (0.5506). The most common genotype occurring in the gene LEP was heterozygous genotype (AG) and the least frequent genotype in LEP was AA (0.1899). Taking the age into account the BMI is higher if the G allele occurs in the LEP gene. Moreover, if the G allele genotype was situated in dominant form, then the highest average BMI was present.
According to the results we can assume that the AA genotype (LEP) has a protective effect on the prevalence of obesity compared to the other genotypes.
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Industrial Utilization of Grasses
131-134Views:64The Agricultural Research and Development Institute P.U.C., Szarvas – realizing the importance of using the biomass in a number of ways – was one of the first in Europe, who began the breeding of grasses suitable for industrial utilization. The possible areas of using the energygrass – which could be called industrial grass – are the following:
– Energetical use
– Paper industry use
– Utilization as an industrial fibrematerial
– Use for feeding
The aim of the breeding programme: developing the kinds of grass, which yields big drymaterial mass and suitable for industrial utilization. These kind of grass, taking soil-utilization, economicality and environmental protection, offer and ensure new marketing perspectives and employment facilities for the disadvantageous areas. -
Polymorphism of the bovine GH and LEP genes in a population of Slovak spotted bulls
19-23Views:91The aim of this study was the detection of polymorphism in the bovine growth hormone and leptin genes using the PCR-RFLP method. A
polymorphic site of the growth hormone gene (Alul loci) that results in amino acid change at position 127 of the protein chain (leucine, L to
valine, V) has been linked to differences in circulating metabolites, metabolic hormones and to milk yield. The polymorphism in bovine leptin
gene is situated in the intron between two exons, which results in an amino acid change at position 2059 of the protein chain (cytosine, C to
thymine, T). The polymorphisms were studied in a group of 58 bulls of the Slovak spotted breed. A strategy employing PCR was used to amplify 428 bp (GH gene) and 422 bp (LEP gene) products from blood samples. Digestion of PCR products with restriction enzymes AluI and Sau3AI revealed alleles: L and V; A and B for GH gene and LEP gene, respectively. The growth hormone gene is a candidate gene for body weight gain in cattle, since it plays a fundamental role in growth regulation. Leptin plays an important role in the regulation of feed intake, energy metabolism, growth and reproduction of cattle; therefore, animals with higher leptin gene expression will probably have lower daily weight gain than others with similar forage offer and nutritional condition and will also likely have longer calving intervals. -
‘Kindergarten’ keeping-system in farrowing house: effect the socialization of piglets on weight performances, fecal cortisol metabolite level and post-weaning behavior
167-174Views:151This study aimed to examine whether litters’ let-together system before weaning (‘kindergarten-system’) has any stress effect and effect on post-weaning behavior, respectively. One week before weaning piglets were socialized by removing four adjacent farrowing crate walls. After weaning, piglets from the same experimental groups were housed in the same rearing crates. The piglet’s body weight was measured at birth than weekly till the 7th life-week. Fecal cortisol metabolites were measured for evaluation of the adrenocortical activity. Personal observation and evaluation were carried out for behavioral analyses after weaning. There were no significant differences in weight development and cortisol metabolite levels between the control and experimental groups. Behavioral analysis showed that pigs grow up in a socialized system, rapidly evolve ranking in the rearing crates, and the self-maintenance and social behavior were more pronounced. In control groups, the activity involving movement (exploring, walking, and running) was much more decisive. Recent work suggested the beneficial effect of the ‘kindergarten’ system in the farrowing house in animal welfare aspects.
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Using phenotypic correlations in the breeding evaluation of Hungarian Sport Horse mares
38-43Views:103The aim of this paper is to analyse phenotypic relationships between self performance test (SPT) parameters of Hungarian Sport Horse mares. Establishing these relationships may contribute to develop a breeding value estimation model. Data were given by the Association of Hungarian Sport Horse Breeders.
The analysis is based on the SPT results of 3 and 4 year old mares from 1993 to 2004. Our aim is to determine practically explainable correlations among conformational traits (type, head, neck, saddle region, frame, forelegs, hind legs, regularity of movement, impulsion and elasticity of movement, overall impression); free jumping performance (jumping style, jumping ability, observation during training) and movement analysis traits (walk, trot, canter, overall impression, test rider’s score). A further aim is to determine correlations between free jumping performance and movement analysis traits. -
Sporthorse performance testing in eventing by own and progeny performance
49-56Views:125The aim of the study was to evaluate the Hungarian Sporthorse population based on eventing competition performance. The database contained the results of 792 horses and 449 riders between 2000 and 2006. The eventing results were gathered from Hungary and other European countries. Blom transformed ranks were used to evaluate the sport performance.Three models were fitted to the Blom scores. Evaluating all the competition categories at the same time weighted Blom scores were used according to the difficulty of the category. The linear mixed model included fixed effects for age, sex, breeder, owner, location, year; and random effects for animal and rider. Horses from the database were judged by their own performance, and stallions were investigated by performance of their progenies on the basis of descriptive statistics of Blom scores and weighted Blom scores. Breeding values of eventing performance were predicted. To improve the reliability of breeding values, more progenies should be
used in eventing competitions. -
Evaluation of egg quality parameters of two Hungarian ostrich populations
51-57Views:198The aim of our study was to evaluate the quality parameters, porosity and weight loss of eggs deriving from the two most significant ostrich farms in Hungary. Quality parameters included weight, length, width, shape index, egg volume, surface area, circumference and shell volume. The effect of storage conditions in both farms and the incubation technology on egg weight loss in farm “A” were also examined. The research objective was to impart a comprehensive knowledge on egg quality parameters of the main ostrich populations in Hungary and to compare the trios and the farms with each other and the international literature. We could reveal significant differences between trios in all egg quality traits. In conclusion, the shorter and the narrower the eggs were, the more spherical shape they had. Narrower eggs showed smaller surface area, volume, circumference and shell volume and vice versa. Eggs from farm “B” indicated significantly greater width, shape index, surface area, circumference and shell volume than farm “A”. A significant difference was observed in weight loss during storage between the farms. Weight loss in farm “A” was a multiple of farm “B”. In farm “B” there was a weak, positive correlation between storage period and weight loss (r=0.22, P≤0.05), in farm “A” it was not significant (P=0.52). There was no relationship between the initial egg weight and weight loss either in farm “A” or farm “B” (P=0,21, P=0,69). A slight positive correlation could be noted between egg porosity and weight loss (r=0.24, P≤0.05). Pores count presented here was less than the international results. Poultry eggs contain the most pores at the blunt end, less via the equator and the least at the pointed end. In ostrich egg we found more pores via the equator against the blunt end. To draw more precise conclusions, further investigation should be carried out on porosity. Considering the fact that the length of storage period and the weight loss during incubation are in strict correlation with hatchability, we intend to extend our research aims to these traits.
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Lambing Frequency in the Ile de France, Suffolk and Babolna TETRA Stocks of Babolna c.o., Szendrő
29-32Views:115Lambing frequency of three different genotypes was evaluated in the breeding stock of Babolna c.o. Szendrő. Ile de France, Suffolk and Babolna TETRA breeding ewes gave the data for the further evaluation. The main aim of this research was to find out if there is any significant effect of selected parameters (registration class, lambing season, lambing type and the years) on the lambing frequency, the period between consecutive lambings. Production parameters of 9,471 Ile de France, 1,244 Suffolk and 7,618 Babolna TETRA ewes were collected and processed. The analysis covered the period between the years 1989-1999. It was concluded that not all of the evaluated parameters had a significant effect on lambing frequency. Not all of the genotypes showed significant differences, according to the examined parameters.
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Origin, history and utilization possibilities of Carpathian Braunvieh at Hungary
15-20Views:166The Carpathian Braunvieh was established by the cross-breeding of Braunvieh and the small dun mountain breeds of the Carpathian basin. It has been breeding in Hungary about 100 years before and still living in Transcarpathia and Transylvania, but only in very mixed populations. Since it was a characteristic native cattle breed of the country, it should be retain for future generations and re-establish in Hungary. Our primary aim is to preserve the breed’s genetic resources and - if appropriate - to acclaim it as an indigenous breed. If there are no longer residuals of the old type Carpathian Braunvieh, it is still worth to preserve as a local variety, because of its many favourable features. Above all, it’s our duty to maintain the breed, because neither Transcarpathia nor Transylvania has a breeding programme for it.
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Comparison of RAPD and AFLP Analysis in Some Maize (Zea mays L.) Lines and Hybrids
3-7Views:88The use of molecular markers to enhance plant breeding efforts is being widely studied. DNA-based fingerprinting technologies (RAPD and AFLP) have proven useful in genetic similarity studies. We estimated different maize (Zea mays L.) inbred lines and hybrids originated from mutant ones based on their genetic differences.
We carried out RAPD analysis with different primers and the 707 (CCCAACACCC) and 792 (CAACCCACAC) primers with 50% similarities provided quite good DNA fragments. By applying the DNA based-AFLP technique, we had very dense DNA fingerprinting. We differentiated 15-32 polymorphic bands, the highest number of bands were found in P-T/H-CA (32). AFLP seems to be the more efficient method of comparing genetic similarities/differences among different genotypes. -
Ovarian performance of Barbados Blackbelly and Dorper crossbred ewes in the mirror of body conditions
23-26Views:75The aim of examination was to detect the puberty and to follow the ovarian cycle of Barbados Blackbelly×Dorper ewes in connection with their body conditions and the effects of meteorological parameters. The blood samples had been taken from 17th of November 2008 untill 08th of June 2009 for analyzing the progesteron concentrations, establishing whether they showed perpetual ovarian performance or not. The end of examination
was timed due to releasing the ram in middle of May 2009.
The daily avarage bodyweight-growth is well featured by the bodyweight tendence. The body conditions were evaluated by ultrasound tissue examination.
During the full length of the examination the avarage daily temperature, the avarage humidity level and also the number of daily sunlighted hours were served by the Agrometeorological Observatory of the University of Debrecen.
The bodyweight tendence and the meteorological parameters were correlated to the breeding season of ewes. In conclusion the bodyweights and their tendences at the young age and the body condition parameters measured at certain anatomical areas can influence the length of the breeding season of the ewes. However the number of daily avarage sunlighted hours, the daily avarage temperature and also the humidity did not effect the cyclic ovarian perfomance of the Barbados Blackbelly×Dorper ewes in Hungary. -
Nutrient Uptake of Miscanthus in vitro Cultures
23-24Views:72The large biomass production and the low necessary input fertilizer make Miscanthus an interesting, potential non-food crop with broad applications, e.g. for fuel and energy, for thatching, fiber production, for the paper and car industries, as well as for ethanol production.
Axillary buds of Miscanthus x giganteus were placed on a shoot inducing nutrient solution (modified Murashige and Skoog, 1962), basic medium supplemented with 0,3 mg l-1 6-Benzylaminopurin. After 40 days of culturing, the axillary buds produced three times more shoots than could normally be harvested. The nutrient content (N, P, K, Ca, Mg) was measured several times during culturing. The results showed that, after 35 days, nitrogen and phosphate were nearly completely taken up. From that time, shoot growth was not observed.
After shoot propagation, the plants were transfered into a nutrient solution for root formation (modified Murashige and Skoog, 1962), basic medium supplemented with 0,5 mg l-1 Indole- 3-Butyric acid, and could be potted in soil after about 14 days. -
Gene Bank Developed by Induced Mutation for Selection
45-49Views:68Heterosis breeding in maize caused gene erosion by using uniform inbred lines. In order to strengthen the genetic base, we established a gene bank containing lines with broad genetic variability, resistance and adaptability. The maize gene bank is a result of our work in the past two decades.
The gene bank originated from treatments of maize seeds of hybrids and inbred lines with fast neutrons. The 1500 maize lines have great genetic variability which can be exploited after strict assessment and selection. As a result of the past several years, P 26, P 61 and P 62 lines have been released after DUS investigation in 2001. -
The methodology of the Hooded Crow’s (Corvus cornix L.) colour ringing and the previous results
43-48Views:148The Hooded Crow (CorvuscornixL.) have moved in several Hungarian cities in the last few decades. It is breeding in Debrecen since 1959, and nowdays it’s presence can be detected at all points of the city, it is an permanent breeding species of the bird fauna. Our knowledge about the nature of urban races, including motion patterns, area fidelity is sufficiently incomplete. The aim of our research is to answer these questions by using our colour-ring program. In this study we present in details the methodology of Hooded Crow’s colour-ring program in urban area, and we also report our previous
achievements. -
The effect of feeding different glycerol sources on the performance of lactating sows
99-103Views:161Glycerol is a by-product of the biodiesel industry and it might be a good alternative to moderate the energy deficiency of sows during the lactation period. Preliminary experiments were carried out to test the effect of a powder, solid based “food grade” glycerol source with 72.9% glycerol content (Trial 1) and a liquid “feed grade” glycerol source with 86% glycerol content (Trial 2) on the performance of lactating sows and their litters. Trial 1 was conducted with 5 Hungarian Large White×Hungarian Landrace sows/treatment (313±24.9 kg) and Trial 2 with 12–12 DanAvl (323±17.0 kg) sows and their litters/treatment. Neither the solid, powder based glycerol (Trial 1), nor the liquid glycerol source (Trial 2) had significant effect on the feed intake, reduction in live weight and back-fat thickness, and weaning-tooestrus interval (p>0.05) of lactating sows. In Trial 2, on the 14th, 21st and 27th days of lactation the milk samples were collected and it was found that 50 kg/t glycerol decreased the protein content of milk samples (p<0.05). Glycerol supplementation had no effect on dry matter, fat, lactose content of milk samples (p>0.05). In Trial 2, no significant difference was found between control and experimental sow groups in triglyceride concentration of blood samples and in the activity of liver enzymes (ALT, AST, GGT; p>0.05), but the concentration of plasma glucose and cholesterol increased tendentiously (p<0.10).
Based on our preliminary results, it can be concluded that additional dose trials are needed to perform in order to study the effect of glycerol supplement on milk production and on metabolic processes of lactating sows.
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Effect of the claw disorders on production performance in dairy cattle
15-19Views:158The aim of the study was the influence of claw disorders on production traits in dairy cattle. Observed were claw traits as claw angle, claw length, heel index, claw height, claw diagonal and claw width. Right hind claw after functional trimming was evaluated. Occurrence of claw disorders like interdigital dermatitis and heel erosion (IDHE), digital dermatitis (DD) and sole ulcer (SV) as well as the progress of disease were observed. Basic summary and variation statistics was performed by the SAS software. Two herds were included in the study. Holstein dairy cows (n=101) produced 11 875 kg of milk, 468 kg (3.94%) of fat and 396 kg (3.34%) of proteins. Holstein cows were affected with IDHE in 22 cases, with DD in 6 cases and with sole ulcer in 10 cases. Slovak Simmental dairy cows (n=101) produced 5834 kg of milk, 258 kg (4.44%) of fat and 209 kg (3.59%) of proteins. Slovak Simmental cows were affected with IDHE in 8 cases, with DD in 5 cases and with sole ulcer in 19 cases. Observed was that claw disorders caused deviations of milk production.
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Study of drought stress correlation on yield and yield components of maize cultivars (Zea mays L.)
67-73Views:160This article was investigated to study the correlation and analysis of drought stress regression on maize cultivars' yield and components. The variance analysis results showed a significant difference between drought stress levels in terms of plant height, total dry weight and number of seeds per row, the total weight of cob, grain yield, harvest index, stem diameter, and cob weight with protective leave. Also, there was a significant difference in ear weight without protective leaves, ear diameter, ear length, plant weight, 100-seed weight, and seed per ear on hybrid treatments. There were statistically significant differences between cultivars in plant height, leaf area, ear diameter, ear length, number of seeds per row, number of seeds per ear, the total weight of cob wood, 100-seed weight, harvest index, plant dry weight. The results of the correlation of traits for the mean levels of drought stress showed a positive and significant correlation between plant yield and plant height, seed per row, ear length and weight of 5 pieces of wood and also with a total weight of cob wood, ear weight with bark showed the highest correlation. There is a significant correlation between leaf area and stem diameter, plant weight, total dry weight at the probability level of 0.05. Correlation coefficients between traits in non-stress conditions showed a positive and significant correlation between grain yield and height, ear length and grain in the row, which was a significant increase compared to stress conditions. The correlation of traits under full stress conditions also showed that the correlation coefficient between cob length trait and positive height was positive and significant. From the study of correlation coefficients between maize traits in non-stress conditions, it can be concluded that the most important components of grain yield are cob length and grain per row. While the correlation coefficients under moisture stress conditions show that the grain trait in the row has a positive and significant correlation with yield, under stress conditions in the cob stage did not show any traits with correlation yield.
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Examination of feral pigeon populations at animal breeding farms
52-56Views:81In the course of our examination, we assessed biometric data that determine gender, we evaluated sexual activity, measured the nourishment and necropsies of two feral pigeon populations feeding and nesting at animal breeding farms. The changes in the body weight of the different genders were significant on both farms (p<0.05).
The body weight and the crop volume of the birds were not significantly different at the examined farms.
On both farms, the elemental food source of the birds was the fodder and corn kernels given to cattle, and was supplemented by that of the pigeons living at the Debrecen farm with food from surrounding farms and with other seeds (pea, millet, rye).
By the examination of the genitals, the birds collected from the Nyírbátor farm showed sexual activity from the beginning of spring, and in the summertime decreasing activity is characteristic, while the pigeons from the Debrecen farm are only active from the summer months. In the course of the examinations in the Veterinary Institute of Debrecen, the birds proved to be free of parasites and the results of the Newcastle disease analysis were negative as well. The bacteriologic analyses showed a different proportion of E.coli, Pasteurella multicoda, haemolysing Staphylococcus bacteria at the two cattle farms. During the examinations for the statement of Salmonellas, this causative agent was stated only in case of birds that spend nights and nested in the town, which allude to human origin. We demonstrated Eimeria oocysts during the parasitological examination of the fecal samples in 5 cases at the Debrecen, and in 1 case at the Nyírbátor farm. -
Az évszak és az azonos évszakban történt ismételt mintavételek hatása holstein tenyészbikák ejakulátumának minőségi- és mennyiségi jellemzőire
33-37Views:80This study was carried out at the Hungarian National Artificial Insemination Centre in order to evaluate the seasonal effects on the quantitative and qualitative parameters of sperm production using sperm analyser equipment. The volume of ejaculate (VE), percentage of living sperm (PLS), spermatozoa concentration (SC), motility score (MC), percentage of motile spermatozoa (PMS), speed of movement of sperm cells (SMS) and the percentage of sperm cells moving straightforward (PSMS) were collected and observed in three seasons (summer, autumn, winter) in Holstein-Friesian breeding bulls (n = 15). The sperm collection was done for three successive weeks in every season on the same day (summer: n = 41, autumn: n=39, winter: n = 42, altogether: n = 122). The same sperm samples were measured by sperm analyser equipment (HTM version 7.0, Danvers) for PMS, SMS and PSMS. The seasonal and the number of sperm collection effects were confirmed on semen characteristics by multiple analysis of variance (two ways MANOVA, Type III) using the program package of STATISTICA 4.5. The average values of VE were similar in the three seasons (summer: from 4.42 to 6.28 cm3, autumn: from 4.08 to 6.86 cm3, winter: from 5.43 to 5.71 cm3). The average values of the MC were similar in each of the three seasons (summer: from 3.66-4.00 M; autumn: from 3.66-3.77 M; winter: from 3.86-4.07 M). The summary of all effects for season (P<0.001), repeated sperm collections in the same season (P<0.05) and interaction of two traits (P<0.01) were established on the measured characteristics. The special effects (P<0.05) of a given season were calculated, excepting the VE and MC, on all of the measured characteristics. The special effect (P<0.01) of the repeated sperm collections in the same season was verified in only one case (SMS). It is not surprised at the interaction was established on the VE (P<0.001), PMS (P<0.05), and SMS (P<10.0). The results of present study suggest that seasonal effect on sperm quality of breeding bulls cannot be eliminated even at standard feeding and keeping conditions. Considering our results, we can recommend that the average values of three successive weeks in every season be used, to take advantage of seasonal effect on the quantitative and qualitative parameters of sperm production.