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Unraveling changes in the duck microbiome and inflammatory processes due to allithiamine-enriched feed
163-171Views:54The gastrointestinal tract of poultry harbors a diverse and intricate microbiome that plays a crucial role in nutrient digestion and absorption, immune system development, and enhances resistance against pathogens. Maintaining a healthy state and proper production is fundamentally determined by the symbiosis between the host and microbes. Due to genetic and technological improvements, intensive growth rate can be associated with many pathological conditions, such as increased susceptibility to infections. Intestinal inflammation in poultry industries detrimentally affects productivity by hindering nutrient absorption and the efficient allocation of nutrients for growth. The host releases different biomarkers in response to inflammation. Hence, there is an utmost interest of reliable, precise, sensitive and robust biomarkers to evaluate both the gastrointestinal health status and inflammation in poultry. The aim of this study was to determine how the developed feed prototype (allithiamine) affects the community diversity in raised duck, and the relationship between gut microbiome composition and inflammatory factor as calprotectin, using targeted 16S rRNA gene amplicon sequencing and Chicken Calprotectin ELISA Kit.
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Daily soil carbon dioxide flux under different tillage conditions
141-144Views:191Over the last few years, warming of the atmospheric layer near Earth's surface is increasingly experienced and researchers have also established that concentration of numerous greenhouse gases have risen over the past two centuries value. Change is basically a legitimate process - considering atmospheric concentration as well - but the change experienced during the past centuries could not have become this critical without the contribution of human activity. Due to the nature of the greenhouse effect, the result of a very fragile, complex process is experienced currently on Earth, which can be significantly unbalanced even by a slight change. Carbon dioxide emitted from the soil is involved in the global cycle and has an impact on the greenhouse effect. The rise in soil respiration may result in the further intensification of warming. In the scope of the present study, it was examined how carbon dioxide emissions of the soil evolve over a day. The results have been established based on the comparison of the effects of different parts of the day, tillage methods and irrigation.
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The effect of climatic change on the rheological properties of winter wheat doughs
96-100Views:104In present paper we have examined the effect of climatic change on the extensigraph characteristics of wheat-flours. The baking quality
of winter wheat is largely determined by cultivar, but it can be influenced by weather conditions during growing period. Flours were from 5
cultivars grown at one location in three cropping years. We have found that the extensigraph properties of dough are affected by the weather
conditions, nevertheless, different cultivars distinctly react to the increase of temperature and decrease of precipitation. In generally, the
higher average day temperature and lower precipitation level is favourable to produce winter wheat flour with better quality -
Study of the rams’ jumping order
59-62Views:124The bevahiour of Babolna Tetra, Ile de France and Suffolk rams was studied at semen collection by artificial vagina. The sequence of rams coming out of the group to the place of semen collection and the number of jumpings needed to ejaculation were observed. It was investigated if there were changes in these characters at different occasions, and did they show connection to the body condition. The number of jumpings before ejaculation was different in the different breeds. Semen was taken at the first jump from 63% of the Suffolk, 56% of the Babolna Tetra and 55% of the Ile de France rams. There was no early ejaculation at the Ile de France, but it was common among the Suffolk rams. The greatest changes in the jumping order between different semen collections were observed in the Babolna Tetra breed. There was a medium correlation in the Suffolk breed between the jumping order and body condition in the autumn and spring, which was tight and significant in winter.
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The effects of education, culture, research and development in Hajdú-Bihar county
87-90Views:187The most important instrument of social advancement and economic development is the education system. The educational qualification of the population of Hajdú-Bihar county is continuously improving, however the census data of the year 2001 show that the educational qualification of the population of the county did not reach the national average. The educational qualification of the county’s population is higher at the county seat and in other cities and lower in small municipalities. The county’s ratio is strongly influenced by the data of the county seat Debrecen and that of Hajdúszoboszló sub region.
The unfavorable educational level of the region is related to the migration tendencies and the brain-draining trends of the past decades, as a consequence of which the population of the less developed eastern regions has moved either to the capital, or to the county seats, or larger cities of the region. The young are increasingly affected by the process.
The number of kindergartens in the county corresponds to the national average, therefore taking into consideration the regional demographic characteristics the aim should be the equalization of regional disproportions. The number of students per 1000 resident within the Northern Great Plain Region and in the county significantly exceeds the national average. The secondary school network of the county consists of 38 secondary grammar- and 51 secondary vocational schools.
The secondary schools of Debrecen have considerable impact on the county’s secondary education. The vocational training network of Hajdú-Bihar county is a large, in its conditions highly differentiated institutional network in which all types of secondary vocational institutions are present with secondary technical-, vocational-, accredited higher vocational-, specialized or preparatory vocational-, and special skill enhancement programmes. The majority of the secondary institutions have regional, rarely national enrollment.
Students who have the intention to continue their studies in higher education within the county can study in Debrecen or Hajdúböszörmény.
The integrated University of Debrecen has the highest student number within the country and it offers the widest range of academic programmes in Hungarian higher education. The University of Debrecen with the wide range of graduate and postgraduate educational programmes, unified academic qualification system, English language programmes is a European standard scientific centre of the Trans-Tisza Region. The county’s higher education takes exceeding share beyond its weight in the education of Natural Sciences (Biology, Physics, Chemistry, Earth Sciences, Mathematics), Health- and Social Sciences, Agricultural- and Environmental Sciences and Information Technology, which positively influences its educational structure. The county’s share in the education of Humanities (Hungarian Language, Foreign Languages, History, Archeology, Philosophy) is also significant.
6.3% of the country’s scientific researchers work in the county. In 2010 7.0% of the country’s R+D expenditure was spent here. 3/4 of the region’s research capacity is in Hajdú-Bihar county employed either by the University of Debrecen or by the research teams of the Hungarian Academy of Sciences.
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Risk effects of the spread route of mycotoxins
90-95Views:137In Hungary the mycotoxin is a great problem, because there are many natural toxins in wheat and maize. These cereals can be found on
considerable proportion of the country’s sowing area, and they are deterministic food for the population. The direct human and animal
utilization of the contaminated cereals mean a serious risk in the food chain. In Hungary’s climate the soil is contaminated with pathogen
moulds, particularly Fusarium species, which increase by respective temperature and moisture content in cereals. The Fusarium can
decrease the quality of the wheat in different ways: decrease the germination capability and cause visible discoloration and appearance of
mould, reduces the dry material and nutrient content of the grain. From the toxins produced by the Fusarium genus, the trichotecene (T-2,
HT-2, deoxinivalenol, nivalenol, diacetoxyscxirpenol, Fusarenon-X) and the estrogenic zearalenon (F-2) are the most common in Hungary.
The fumonisins (FB1, FB2, FB3) first identified in 1988, relatively newly discovered, are also important. Major proportion of mycotoxins in a
healthy organization is metabolized by the enzyme system of liver and intestinal bacteria. The toxicity is reduced or even leaves off.
However, more toxic and biologically active compounds can be formed. For the reduction of mycotoxin-contamination several possibilities
are available in the case of storage, processing and feeding. -
The impact of population management on urban and rural Hooded Crow populations
119-123Views:97Hooded Crow (Corvus cornix), originally native to agricultural areas, has become widespread in urban areas in recent decades. However, this process has negative consequences on urban animals and humans. Due to these problems, the control of urban crow populations is needed. Crows also cause significant damage to wildlife management, and are constantly being controlled in rural areas as well. In this study, we compare rural and urban populations to find out whether hunting activities have a population reduction effect. In the rural population, the reduction is carried out by weapons and traps, while the urban crows are controlled using traps only. In both sample areas, crow nests were surveyed during the nesting period. 29 active nests were monitored in the rural area in 2021, and 39 in 2022. In the urban area, 44 nests were recorded in 2021 and 35 nests in 2022. In 2021, 30 individuals were removed in the rural area, and 84 in 2022. In the urban area, 223 individuals were removed in 2021 and 144 in 2022. Results show that the number of crows removed follows the number of active nests, so that the reduction of a given year is likely to have an effect on the following year's nesting population. Because traps mostly capture juvenile birds, reducing the breeding population in the city can only be achieved in the long-term. Considering this, it is likely that increased attention to population control can effectively maintain crow populations and reduce the problems they cause.
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Evaluation some important microbiological parameters of the carbon cycle in chernozem soils profiles
33-39Views:173Some chemical and microbiological properties of the carbon cycle were investigated in three chernozem soil profiles. The soil profiles originated from a long term fertilization experiment (potato) of the University of Debrecen, Látókép, Kryvyi Rig Botanic Garden (grassland) and a large-scale farm (sunflower) of Ukraine. The results of the organic C-content, total number of bacteria, microscopical fungi, cellulose decomposing bacteria, CO2-production, microbial biomass carbon and saccharase and dehydrogenase activities were compared and evaluated with the help of correlation analyses. Close correlation was found between the organic carbon content and the number of microscopical fungi,, saccharase and dehydrogenase enzymes’ activities, as well as close correlation was found between the dehydrogenase activity and microbial biomass-C and saccharase activity.
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Deproteinized plant juice as part of circular economy: A short review and brief experimental data
23-26Views:1508As the population of the Earth is constantly growing it generates an unmet demand for protein, which is an urgent problem. The protein extraction process is a potential solution, which offers high-quality plant protein suitable for animal and human nutrition at a favorable price. The process used within our project produces green juice from the green alfalfa biomass through pressing. After the coagulation of protein from this green juice, the by-product is called DPJ (Deproteinized Plant Juices) or brown juice. Our preliminary results match the international literature, namely that brown juice take up as much as 50% of the fresh biomass in weight. To utilize this by-product is a crucial part of the process to make it environmental-friendly and financially viable as well. The examined brown juice samples came from a small-scale experiment of alfalfa varieties carried out in the experimental farm at the University of Debrecen. According to our preliminary results, brown juice has high macro- and micronutrient values, furthermore, it has a potentially high amount of antioxidant compounds. The study highlights that brown juice is suitable as an ingredient in microbiological media, in plant nutrition as a supplementary solution, for feedstock and for preparing human food supplements or functional foods. The potential utilization of all biorefinery products makes it a very appropriate technology for today’s challenges.
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Will there be a horticultural triangle (cluster)? Thoughts about the reconstruction of the Hungarian horticulture between two European regions
21-27Views:147The authors of this study seek the answer to the question how to develop, in the first decade or decades of the 21st century, the university-level
horticultural scientific training, the horticultural innovation and the scientific co-operation between companies and universities in Debrecen and
in the North Great Plain Region and – in a wider sense – in Hungary to a standard being competitive even in European terms. With the synthesis
of the prospects of past, present and future, they drew the following conclusions. The reconstruction of agriculture, horticulture and food industry
is a part of reforming Hungary's countryside. Horticulture, producing high added value, will be able to decisively contribute to the plan whereas
the value presently produced in an agriculturally cultivated area of 1,000 euros/hectare can reach 2,000 to 3,000 euros in the next two decades.
A necessary and indispensable precondition to achieving this is the strengthening of the innovation output of the Hungarian horticultural sector.
Despite the numerous technical criticisms formulated in connection with the serious problems of Hungarian agricultural and horticultural
scientific innovation, no progress has been made in this field for the past one and a half decade. The scientific research of this topic hardly
continued or did not continue at all, the up-to-date surveys and in-depth analyses were missing. The objective, basic principles and tasks of the
Act CXXXIV of 2004 (TTI) enacted concerning research-development and technological innovation are clear and progressive. The co-operation
between the National Research Technology Office and the Hungarian Academy of Sciences, the setting up of the Innovation Fund are heartening
opportunities. These – along with the new Higher Education Bill to be passed – may as well be suitable for restarting the Hungarian agricultural
and horticultural scientific innovation. In our opinion, this requires a new, well-considered national agricultural programme, which can be
conceived in the framework of the "Ferenc Entz National Horticultural Plan" proposed by us for horticulture. In the most eastern Hungarian
university knowledge centre, at the University of Debrecen, the continuing of the horticultural scientific innovation strategy started in the last
decade may be the focal point and generator of the development of the so-called "Hungarian Horticultural Triangle”, or "Hungarian
Horticultural Cluster". This region comprises the Northern and Southern Great Plain Regions and the area between the Danube and Tisza
Rivers. Here, about 70 to 75% of the total Hungarian horticultural commodity stock is produced. The objective of the HORT-INNOTECH
DEBRECEN programme planned in 2004 by the University of Debrecen, Centre of Agricultural Science is to establish the horticultural scientific
research-development and technological innovation structure and knowledge base of the Hungarian Horticultural Triangle / Hungarian
Horticultural Cluster. In harmony with this, the objectives are to bring about competitive, new horticultural products, to improve the conditions
of utilising them, to enhance the competitiveness of enterprises based on technological innovation, to make use of the research-development and
innovation opportunities available in the regions in an efficient manner, to as full extent as possible, to encourage the creation of places of
employment producing high added value in the field of horticulture, to improve the technical skills of those employed in horticultural researchdevelopment and to promote their enhanced recognition by the society. -
Effect of Ferilizer on the Yield of Maize (Zea mays L.)
40-46Views:129The effect of fertilization on the yield of maize was examined on chernoem soil with lime deposits at the experimental station at Látókép of the Center for Agricultural Sciences, University of Debrecen. The yields of maize were evaluated using quadratic regression function, in three years – between 2000 and 2002 – in non-irrigated and irrigated treatments. After calculating the regression equations, by derivation of the functions, we have determined the amount of fertilizers needed for maximum yield.
In the non-irrigated treatments, maximum yield and the active substance amount of fertilizer was as it follows: in 2000, yield of 9,133 t/ha with the application of 384 kg/ha mixed active substance, while in 2002 a yield of 6,289 t/ha with the application 236 kg/ha NPK active substance was achieved. In 2001, due to the favourable precipitation, a yield of 9,864 t/ha was achieved with the application of 245 kg/ha fertilizer. In the case of maximum yield, compared to the unfertilized control, the yield increase was 2,5-5 t/ha. The average increase for 1 kg of NPK fertilizer was 13-19 kg.
We also determined the necessary fertilizer dosage for maximum yield in irrigated treatments. In 2000, 10,003 t/ha with a dosage of 423 kg/ha, in 2001, 11,542 t/ha with a dosage of 277 kg/ha and in 2002, 8,596 t/ha of maximum yield could be achieved with a fertilizer treatment of 277 kg/ha in the examined three years. The yield increase, in irrigated treatments, varied between 3,9-5,9 t/ha so it was greater than in the case of non-irrigated experimetal plots. The yield increase for 1 kg fertilizer varied between 12-21 kg. -
Examining the production parameters of European perch (Perca fluviatilis) juveniles under different lighting conditions
149-153Views:184European perch (Perca fluviatilis) is a native predatory fish in Hungary, and a promising new species of fresh water aquaculture nowadays. The European perch can be characterized by a high stress sensitivity during the intensive rearing, thus the optimization of environmental conditions has significant importance in the early life stages of fish. The aim of our study was to determine whether the light intensity and darkening of the water by humic acid affect the survival and growth performance of European perch juveniles. The experiment lasted for 28 days. Rectangular aquariums were arranged in 3 lines, the test environment consisted of 24 units. The experiment was set up with 10–10 fish per aquarium, total of 240 individuals. The average wet body weight of the fish at the start of the experiment was 1.69 grams. During the experiment, 6 treatments in 4 replicates were set up. In the first row, we set up 305.6±66.0 LUX (L), in the second row 118.0±24.4 LUX (C), and in the third row (D) 17.0±8.6 LUX illumination was set up. In each row the water of four aquariums were darkened by the addition of humic acid (H) (L, LH, C, CH, D, and DH). At the end of the experiment high survival rate was observed in all treatments. Examining the survival rate, the best results were found in the strongly illuminated treatments (L; LH- SR%=100±0.00). The observed mortality was caused by cannibalism. Regarding the individual body weight, the best results were shown by the treatments where the aquariums were illuminated with lower light intensity (C: 4.66±0.33 grams) and the aquarium water was darkened by humic acid (CH: 4.93±0.15 grams). The results of the C and CH groups were significantly better compared to the other treatments, however, they did not differ statistically from each other. The darkening of the water had a positive effect on the individual body weight of fish reared under full light (L: 3.73±0.28 grams; LH: 4.33±0.28 grams), whereas in the case of fish reared in the lowest illumination, the addition of humic acid did not affect the results (D: 3.78±0.15 grams; DH: 3.80±0.26 grams). In case of SGR (C: 3.64%/day and CH: 3.74%/day) and FCR (C: 0.84 g/g and CH: 0.78g/g) the best results were obtained by the C and CH treatments, also.
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Changes of some soil chemical and microbiological characteristics in a long-term fertilization experiment in Hungary
253-265Views:302Agricultural management practices – directly or indirectly – influence soil properties.
Fertilization rates and crop rotation can strongly affect soil pH, soil nutrient supply and soil organic matter content due to the changes of microbial processes. The objective of this study was to compare the effects of different fertilization doses in monoculture and tri-culture of maize (monoculture: only maize grown since 1983, tri-culture: it is a three-year crop rotation system: pea – winter wheat – maize) on selected soil characteristics. The long-term fertilization experiments were set up in 1983 in Eastern Hungary. These experiments are situated west of Debrecen in Hajdúság loess region, on calcareous chernozem (according to WRB: Chernozems).
The test plant was maize (Zea mays L.). One-one pilot blocks were selected from monoculture and tri-culture of the long-term experiments. The observed soil samples were taken in the 30th year of the experiment, in 2013. The doses of NPK fertilizers increased parallel together, so the effects of N-, P- and K-fertilizers cannot be separated.
With the increasing fertilizer doses, the soil pH has decreased in both crop production systems and, in parallel, the hydrolytic acidity has significantly increased. A close negative correlation was proved between the pHH2O, pHKCl and hydrolytic acidity. An increased nutrient content in soil was recorded in every NPK treatment and the available phosphorus and nitrate content increased in higher proportion than that of potassium. Of the measured parameters of C-and N-cycles, fertilization has mostly had a positive effect on the microbial activity of soils. Besides the effects of fertilizer doses, correlation were looked for between soil microbiological properties. Evaluating the ratios among the measured parameters (organic carbon and microbial biomass carbon, OC/MBC ratio; carbon-dioxide and microbial biomass carbon; CO2/MBC proportion), the fertilization rate seems to be favoured by the increase of amounts of organic compounds
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Preliminary data on the effect of semi-synthetic baits for Noctuidae (Lepidoptera) on the non-target Lepidoptera species
71-80Views:212Noctuidae are one of the most important Lepidoptera groups containing dangerous pest species. Monitoring and detection of these pest species is routinely performed by traps baited with sex-pheromones. Baits that attract both males and females were developed for improved pest management. First the effectiveness of different synthetic compounds was evaluated. We also tested semi-synthetic baits that contained both synthetic and natural components (wine and beer). These were more attractive for moths considering species richness and abundance. Disadvantage of this increased effectiveness is that the traps catch more non target, rare and even protected species. In this study we analysed the effect of semi-synthetic baits developed for Noctuid moths containing wine on other non-target Lepidopterans. In the six sampling sites traps caught 17158 individuals of 183 Lepidoptera species. The number of Noctuidae species was 124, while their proportion was 84.4%. The traps caught 813 individuals of 9 protected and 20 valuable species, which was only 4.7% of all Lepidopterans. In contrast the mean proportion of 33 dangerous and potential pest species was 31.3% (5375 individuals). Number and abundance of both protected and pest species were affected by landscape structure. The risks of catching non-target species was higher in species rich natural and semi-natural landscape. In homogenous arable lands the number and proportion of valuable Lepidopterans was not significant.
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Examination and statistical evaluation of physico-chemical parameters of windrow composting
33-38Views:245The treatment and utilization of plant and animal waste and by-products from agriculture is very diverse. Traditional environmental management practices for waste management have been retained through soil conservation and the applied of recycle degradable organic substances in soil. The management of by-products from agriculture (animal husbandry) is important because a closed loop can be created to utilize by-products (manure, feathers) from the production of the main product (eggs, meat, milk) and to form a raw material for a new product. It is important to treat the resulting by-products, especially deep-litter manure, as it has served as a basis for compost-treated manure to develop an organic-based, soil-conditioning product line. Poultry manure by itself is not suitable as a substrate for aerobic decomposition, so it has to be mixed with other substances (zeolite, bentonite, soil), because of its high nutrient capacity, it is an acidifying substance.
The aim of this study was to compost the mixture of poultry manure and hen manure by the addition of zeolite and to monitor the composting process. It was also our aim to statistically determine the effect of the zeolite on parameters describing the composting process.
The windrow composting experiments were set up in the composting area of the University of Debrecen, Institute of Water and Environmental Management. The composting experiment was 62 days long, during which the main parameters describing the composting process were continuously monitored: temperature (°C), moisture content (w/w%), electrical conductivity (mS/cm), organic matter content (w/w%), examination of nitrogen forms (w/w%). In this study, three factors were investigated: temperature, humidity, and pH. For statistical evaluation, R software and RStudio user interface were used. We developed a repeated measurement model, in which the fixed and random effects were determined for our parameters under study, and the resulting relationships were shown on interaction plots.
Based on our results, the temperature of the prisms has become independent of the ambient temperature and the composting stages can be separated in both the control and the zeolite treated prisms. In the repeated measurement model, we proved that treatment, time and treatment: time interaction were significant at both temperature and pH.
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The study of the fertilizing effect of wheat straw ash in a greenhouse experiment
47-51Views:192The effect of wheat straw ash as a fertlizizer was studied in a pot experiment with an acidic sandy loam soil (pHKCl=4.9) with weak K and P supply. The test plant was ryegrass (Lolium perenne). The treatments were the following: 1. control untreated soil, 2. NPK fertilizer, 3. small dose of ash (1.4 g kg-1), 4 large dose of ash (2.8 g kg-1), 5. small dose ash completed with NP fertilizers. Soil parameters (pHH2O, pHKCl, ammoinum-lactate soluble P, K, 0.01 M CaCl2 soluble PO43--P, K, Mn, Cu, Zn ) and plant parameters (yield, P, K, Ca, Mg, Zn, Mn uptake) were investigated. Based on the analysis of the straw ash sample and the results of pot experiment it can be stated that the wheat straw ash is suitable for the fertilization of the studied soil. The small dose ash completed with NP resulted in the largest yield increment (43%). In order of the treatments the pHKCl changes to: 4.9, 4.8, 5.2, 5.8, 5.1. As the N : P2O5 : K2O ratio is 0 : 1: 3.5 in the wheat straw ash sample, to reach optimal yield ash should be completed with N and P.
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Identification of cow’s and buffalo’s milk and dairy product using a DNA-based method
279-282Views:192Aim of our study was the optimization of a DNA method, that is appropriate for reliable, low cost identification of animal species in milk and dairy product (cheese) and to determine the ratio of species. Mitochondrial DNA was used in our work to analyse buffalo/cow milk mixtures contained different ratio of bovine milk such as 0.1%, 0.5%, 1%, 1.5%, 2%, 5%, 10%, 15% (v/v%). Buffalo cheese were produced using buffalo and cows milk (0%, 2%, 5%, 10%, 15% – v/v% cows milk in buffalo milk). In case of milk mixtures, using species specific primers, the PCR assay showed a 0.5 v/v% detection limit. Cattle, in the buffalo/cows milk 99.9/0.1 v/v% mixture, was not detectable. The identification of buffalo and cows DNA in cheese was successful. The intensity of eletroforetic PCR fragment indicated the increase of cow milk ratio in milk and cheese samples as well.
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Biological control of sweet chestnut on Pécsbánya, Hungary
77-81Views:219The supervision of plant hygiene of sweet chestnut grove on Pécsbánya (South Hungary) started more than four years ago. Hypovirulent strains were applied as a biological process to control Cryphonectria parasitica fungus which causes the chestnut blight disease. By now the performed interventions have shown obvious results, the vitality of the trees has greatly improved, the amount of harvested nuts is increasing, and the hypovirulent strain has been spreading within the area. During plant health inspection the galls of chestnut gall wasp (Dryocosmus kuriphilus) was found in the year of 2015, which is the obvious symptom of new occurrence of the pest. The pest was eradicated by destroy galls, which allows taking out of consideration the damage by now in this area.
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Evaluation of chickpea (Cicer arietinum L.) in response to salinity stress
105-110Views:262Soil salinity is a severe and expanding soil degradation problem that affects 80 million ha of arable lands globally. Chickpea (Cicer arietinum L.) is very sensitive to saline conditions; the most susceptible genotypes may die in just 25 mM NaCl in hydroponics. Approximately 8–10% yield loss in chickpea production is estimated due to salinity stress. However, it is still not established why chickpea is so susceptible to salt affection. Salinity (NaCl) impedes germination of seeds, though chickpea varieties considerably differ from one another in this respect. Some chickpea genotypes are more tolerant in the stage of germination, tolerating even 320 mM NaCl. The reasons of this variation are unrevealed; there is a shortage of knowledge about the germination abilities of chickpea genotypes in saline conditions. Nevertheless, the effect of salt stress on vegetative growth can be analysed in hydroponics, in pot or field conditions, regardless the experimental environment, the ranking of genotypes regarding salt resistance is coherent. Chickpea genotypes can be different in their ability to retain water, maybe under salt affection; the more salt tolerant lines can maintain higher water content in the shoots, while the more sensitive ones cannot. The identification of salt tolerant chickpea landraces based on developing genetic variability is a suitable strategy to combat against salinity problems arising in arid and semi-arid areas.
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Coagulase-negative staphylococci in ewe udder surface and raw milk samples
221-225Views:159Coagulase-negative staphylococci (CNS) are among the major causes of subclinical mastitis in dairy ewe flocks. This has a financially significant impact on the ewe dairy sector and consumer health. The determination of the bacterial count, particularly CNS, is critical in terms of the quantity and quality of ovine milk. Thus, the purpose of this study was to quantify the CNS count in the udder surface and raw milk samples of the ewe, in addition to identifying CNS strains (n=8) collected from udder surface and individual raw milk samples by 16S rRNA gene sequencing. A total of 164 samples of udder surface and raw milk originated from four sheep farms were tested. The obtained values were compared between the different farms. Furthermore, values during 2018 and 2019 in the case of Farm I were compared. Significantly higher (p<0.05) average count was observed in udder surface samples taken from Farm I (2.8±1.0 lg CFU/cm2) than that of Farm III samples (2.3±0.6 lg CFU/cm2). In the case of individual raw milk, the higher value was observed in samples derived from Farm III (3.5±0.9 lg CFU/mL), while the obtained value was significantly lower (p<0.05) in samples originated from Farm IV (1.8±0.4 lg CFU/mL). In the bulk tank milk samples, the highest mean value was 5.3±0.4 CFU/mL, and there was no significant (p>0.05) variation between farms. Coagulase-negative staphylococci counts were decreased to a certain extent in both sample types tested during 2019 except for individual raw milk derived from the Tsigai breed. The correlation between the mean CNS counts of udder surface and individual raw milk was very weak (r=0.048). Staphylococcus simulans, Staphylococcus auricularis, and Staphylococcus equorum were identified by molecular sequencing and Staphylococcus simulans were the most frequently identified CNS species. A higher CNS count of bulk tank milk than individual raw milk indicates possible contamination during milking and storage. Therefore, further studies are required to investigate the other sources of bulk tank milk contamination to improve the hygienic quality of milk.
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The Effect of Year and Irrigation on the Yield Quantity and Quality of the Potato
12-16Views:104In Hungary, the growing area of potato area reduced dramatically in the last few decades, additionally we are lagging behind the Western European countries as regards yields and the competitiveness of production is further decreased by the great alternation in yields from year to year, the unpredictable market conditions, bad consumption habits and many times unfortunately the lack of quality products.
The ecological and climatic conditions of Hungary are not everywhere suitable for potato, in the area of Debrecen the amount of rainfall was lower, and the monthly average temperature was higher than the requirement of potato in its growing season in 2002 and 2003.
The experiment was carried out at the experimental site of the University of Debrecen, Farm and Regional Research Institute, at Látókép. In our experiment we examined the yield and some quality parameters of 8 and 9 medium-early varieties in large parcels in 2002 and 2003 respectively. Out of the examined varieties 3 are of Dutch, and 6 are of Hungarian breeding.
The experiment was set up on 49.5 m2 parcels on calcareous chernozem soil after winter wheat as a forecrop in both years. The 9 varieties were examined in 4 repetitions in randomized blocks, out of which two repetitions were irrigated, and two were non-irrigated.
We examined the yields of the varieties, the distribution of tubers according to size and their percentages, and the changes in specific parameters of quality and inner content due to irrigation. We studied the dry matter content, the starch content, the under-water mass, the amount of reducing sugars, the colour index of frying and the element contents of tubers.
Summing up, it can be stated that among the agrotechnical year effect, variety and irrigation factors have considerable impact on potato yield quality and quantity. On the basis of our results, it can be stated that in potato production variety should be chosen in accordance with the aim of production and technology should be adapted to that specific variety. -
Comparison of Pálfai’s drought index and the Normalised Precipitation Index in the North Great Plain region
59-64Views:194Agriculture has always been an important role in economy, food supplies, sustainability of society and creation of job opportunities in Hungary. Our country has resource-related strength of agriculture, because we have more than 4.5 million ha for agricultural production. Agricultural production can be influenced by several factors, including climate, hydrology, soil conditions and antropogenic impacts. Climate determines the quality and quantity of the crop yields. The climate conditions in Hungary are variable and it shows spatial and temporal extremes. As a result of this, drought have become more frequent in our country (2003, 2007, 2009, 2012), which is reflected in the decline in yields as well. In the present study, Pálfai's Drought Index (PAI) and the Standardized Precipitation Index (SPI) were compared 2003–2012 in Debrecen. The temperature and precipitation data were calculated from data provided by a local meteorological station to work out PAI, while the SPI-3 index values were downloaded from the database of the European Drought Observatory. This allowed to drought assessment in a local and regional scale. Our study was supplemented with SPI-3, soil moisture anomalies, PAI and yields of wheat (Triticum aestivum L.) and maize (Zea mays L.) to evaluating the impact of drought on agriculture.
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Comparative study of special honey products and herbhoneys
117-120Views:364Honey has a positive effect on human body due to its high content of biologically active substances (e.g. monosaccharides, vitamins, enzymes, amino acids, polyphenolic compounds). The properties of honey depend on its botanical origin due to the bioactive plant components, mainly secondary metabolites that are included in honey made by bees from nectar. Herbally infused honeys are delicious products that combine the therapeutic action of herbs and honey. Additionally, herbs can provide nutrient fortified syrup for honey bees and protect them against diseases and other ecological threats.The aim of this study was to define the physicochemical properties of multifloral honey, herb enriched natural honey and herbhoney samples. We measured the moisture content, pH value, electrical conductivity and proline content. Although great diversity was observed in the basic properties of the examined products. In our study, we found that the electrical conductivity shows the significant differ between the groups. All the samples, including the herbhoneys passes the quality standards of honeys. -
Reference genes for livestock gene expression profiling – Literature review
81-89Views:174Quantitative real-time polymerase chain reaction (qPCR) is an essential tool for understanding animal cell’s response to developmental progression or to different experimental conditions at gene expression level. However the reliability of this method heavily lies on proper normalization (measuring a target and a reference gene’s expression from the same sample to correct for technical related variations).
Our literature review aimed to summarize the articles addressing the most important livestock species in regards of reference gene stability used as normalizers for quantitative real-time polymerase chain reaction experiments. Stably expressing reference genes were categorized into 14 distinct groups according to gene function. The number of reference genes tested and the publication numbers according to years and the ranking algorithms were also noted.
Counting showed that genes encoding ribosomal protein components are ranked as most stable in majority of cases and therefore should be taking into account for qPCR stable normalizer gene finding experiments. -
Study of drought stress correlation on yield and yield components of maize cultivars (Zea mays L.)
67-73Views:201This article was investigated to study the correlation and analysis of drought stress regression on maize cultivars' yield and components. The variance analysis results showed a significant difference between drought stress levels in terms of plant height, total dry weight and number of seeds per row, the total weight of cob, grain yield, harvest index, stem diameter, and cob weight with protective leave. Also, there was a significant difference in ear weight without protective leaves, ear diameter, ear length, plant weight, 100-seed weight, and seed per ear on hybrid treatments. There were statistically significant differences between cultivars in plant height, leaf area, ear diameter, ear length, number of seeds per row, number of seeds per ear, the total weight of cob wood, 100-seed weight, harvest index, plant dry weight. The results of the correlation of traits for the mean levels of drought stress showed a positive and significant correlation between plant yield and plant height, seed per row, ear length and weight of 5 pieces of wood and also with a total weight of cob wood, ear weight with bark showed the highest correlation. There is a significant correlation between leaf area and stem diameter, plant weight, total dry weight at the probability level of 0.05. Correlation coefficients between traits in non-stress conditions showed a positive and significant correlation between grain yield and height, ear length and grain in the row, which was a significant increase compared to stress conditions. The correlation of traits under full stress conditions also showed that the correlation coefficient between cob length trait and positive height was positive and significant. From the study of correlation coefficients between maize traits in non-stress conditions, it can be concluded that the most important components of grain yield are cob length and grain per row. While the correlation coefficients under moisture stress conditions show that the grain trait in the row has a positive and significant correlation with yield, under stress conditions in the cob stage did not show any traits with correlation yield.