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The comparison of several soil cultivation methods in the Tokaj-wine region
35-37.Views:214Our results show that the different cultivation methods have higher effect on the yield than on the grape quality. In 2007 the weather was extreme dry (from May to August only 195 mm precipitation was recorded) the straw mulch proved to be the best solution regarding the yield and the grape quality. Although no significant differences were observed in the sugar- and titratable acidity content of the must, the ratio of noble rotted berries was higher on the straw mulched plots. Straw mulch could conserve the moisture content of the soil and it seems to be contributed to the conformation of suitable microclimate for Botrytis infection, but for long distance conclusions further examinations need to be investigated. The less yield and ratio of rot was observed in case of barely covered vines and the vegetative growth of the grape was less intensive in case of these vines.
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Bacterial diseases of grapevine
45-49.Views:420Grapevines are affected by three major bacterial diseases worldwide, such as bacterial blight (Xylophilus ampelinus), Pierce’s disease (Xylella fastidiosa) and crown gall (Agrobacterium vitis). These bacteria grow in the vascular system of their host, thus they invade and colonize the whole plant, independently on symptom development. Latently infected propagating material is a major factor in their spreading. Therefore the use of bacteria-free planting stock has a basic importance in viticulture. Today several innovative diagnostic methods, mostly based on polymerase chain reaction, are available to detect and identify bacterial pathogens of grapevines. For production of bacteria-free plants, the use hot water treatment followed by establishment of in vitro shoot tip cultures is proposed.
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Valuable offsprings of Seyve-Villard 12375
75-77.Views:209Powdery mildew, Phylloxera and downy mildew epidemics devastated large areas of vineyards in Hungary in the 1800s. To establish new plantages resistant so called "direct producers" were imported from America (direct means "without grafting"). Direct producers have secure yield but their quality is inferior. After World War II, an organized breeding programme was started to replace them and parallel to this the law prohibited their production. As a resistance gene source, the E.2 clone (S. V. 12375 E.2) of Seyve-Villard 12375, Franco-American hybrid selected in Eger was used by Hungarian breeders. Several valuable hybrid families have originated from crosses of this variety hybrid.
In the Research Institute for Viticulture and Enology at Kecskemét, two valuable table grape hybrids were registered under the names of R.65=Esther and R.78=Fanny coming from 2 hybrid families of the parent S. V. 12375 E.2. Both varieties may be cultivated "environmentally".
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Research Note virus transmission by grape phylloxera (Daktulosphaira vittfoliae Fitch)
73-74.Views:133Grape phylloxera (Daktulosphaira vitifoliae FITCH) infests immature roots of both Vitis vinifera L. and phylloxera resistant rootstocks. A capability to transmit viticultural viruses would make grape phylloxera a phytosanitary threat even under conditions where direct damage by the insect is not likely. We tested the hypothesis that phylloxera could transmit grapevine fanleaf virus (GFLV) by planting infected and non-infected vines in common 10 liter pots and infecting roots of the infected vine with grape phylloxera. In this test infection of a previously non-infected plant in the absence of nematode population suggests that grape phylloxera is a vector of GFLV.
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Variability and differences of growth vigour in the set of 36 genotypes of apricot (Prunus armeniaca L.).
30-34.Views:145Growth vigour of 36 apricot cultivars and new hybrids grafted on apricot seedling rootstock (Prunus armeniaca L.) was evaluated on the base of measurements of stem girth from the 411' to the 10th year after planting. There were differences in growth vigour of genotypes under study. In the evaluated set of genotypes the control cultivar 'Veecor may be classified as a genotype with below-average growth vigour. Only four genotypes (-Reale d'Imola-, Sanagian -Moldavskii krupnoplodnyl and 'LE-2385') were found with significantly higher growth vigour than that of control cultivar 'Veecot' in years of the end of experimental period. Two genotypes (Farmingdale', -LE-SE0-24') were found with significantly higher growth vigour only at the beginning of experimental period and one cultivar ('Vivagold-) with significantly lower growth vigour in the first four years. Genotypes with different growth vigour can be used in further breeding programmes and/or as components inhibiting or supporting the growth in indirect vegetative propagation. Within the whole experimental period, the rank of growth vigour of genotypes practically did not change. This was demonstrated by highly significant or significant coefficients of correlation existing between individual pairs of years (r=0.32+ to r=0.96++). As far as the time difference between years in individual pairs of years was higher, the correlation coefficients were lower. In individual years, variability of growth vigour was relatively low and ranged from 9.83 to 13.64%.
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In vitro multiplication and hardening of grapevine plants in aeriated media
15-18.Views:218In vitro cultures have widely been used in horticulture for rapid multiplication of new varieties and clones as well as to produce pathogen-free stock material. To improve efficient hardening and transfer in vitro grown grapevine plants were multiplied by cutting them into single-node internodes with the whole leaf. Microcuttings including the shoot tips were rooted in granulated perlite moisted with tapwater under sterile conditions. After 2-3 weeks the rooted microcuttings were supplied by nutrients and hardened by gradual opening and finally by complete removal of the lids of jars or plastic boxes used for growth. Using this method microcuttings of Vitis vinifera cvs. „Chardonnay", „Cabernet franc", „Riesling" and „Sauvignon blanc" and the rootstock varieties Vitis riparia x Vitis cinerea cv. „Barrier" and Vitis berlandieri x Vitis rupestris cv. „Richter 110" formed new roots and shoots and 100% of the tested plants survived the acclimatization procedure. Similar results were obtained when perlite was replaced with rockwool-, or pit-pot blocks. This method may highly increase the efficiency of producing pathogen-free propagating material and new transgenic lines.
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Evaluation of precision farming with regard to horticulture
105-110.Views:130The global positioning system was made available for public use, which made it possible' to apply a new management tool in agriculture. Precision farming gives much more information on plant-growing than former methods, which makes it possible to use technologies more suitable for micro-sites. It is supposed that more profitable production can be realised with its aid and the strain on the environment can be reduced, not to mention other economic advantages.
The study makes economic conclusions about the method more and more widely used in plant production taking the yield mapping of Józsefmajor Experimental Farm as a basis and starts ideas about its possible application in arable land olericulture, viticulture and fruit production.
These issues are important because precision farming has existed in glass-culture for a long time from another perspective, which should be spread to open ground horticultural enterprises that are labour and asset intensive, qualitative farming forms with great plantation value.
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The effects of foliar nutrition containing various macro and microelements on the growth and development of young grafted walnut (Juglans regia L.) plants
33-37.Views:201The Stopgril liquido (S), a foliar fertilizer containing nitrogen, calcium and magnesium was used to improve the spring and the summer growth of grafted walnut plants in the second year of cultivation in the nursery. To accomplish early termination of vegetative growth and good lignification, the composed mineral fertilizer Hascon M 10 AD (H) containing phosphorous, potassium and microelements B, Mn and Mo was applied on the same plants. Four treatments (S, H, SH, C) were applied three times in a growing season. Their effects on the height and the basal diameter of the plants in the nursery depended on the starting height of the plants and the time of application. During the first growth period in the orchard, the number of developed buds on the plant, the circumference of the plants and their height were influenced by the treatment in the previous year in the nursery. According to the results obtained through the investigation, three applications of Stopgril + Hascon in the nursery per year can be recommended in order to obtain high enough and well lignified two-year-old grafted walnut plants.
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Evaluation of potential of fruit weight and fruit width and their interrelationships in the set of apricot genotypes
47-53.Views:136Within the period of 1994-1999, variability of fruit weight and width was evaluated in altogether 24 apricot genotypes. It is concluded that the genotype and climatic conditions of individual years are the factors causing variability in these two parameters of apricot fruits. Variability of fruit weight was significantly higher (approximately 3-times) than that of fruit width. When evaluated on the base of their width, 75.0% and 95.8% of apricots were classified into the groups of extra and first quality, respectively. As genotypes with the maximum fruit width (i. e. above 50 mm) the following cultivars were classified: 'Velkopavlovicka LE-6/2', 'NJA-1', 'M 45', 'M-25' and lednicka (M-90-A)'. The value of fruit width (in mm) corresponded with its weight (in g) only within the range of 40 — 45 mm. With the increasing and/or decreasing size of apricot fruit the changes in fruit weight were more pronounced than in those in fruit width. Within the set of genotypes under study, this relationship may be expressed by the equation y = 0.1234 — 7.6605 + 152.76; the corresponding values of correlation coefficient and coefficient of curve determination are r = 0.95-H- and R2 = 0.959.
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Microsatellite based identification of grapevine cultivars traditional in Hungary and in the Carpathian Basin
71-73.Views:149Grapevine cultivars and clones traditional in Hungary and in the Carpathian Basin are maintained in the genetic collection of the Corvinus University of Budapest. Mostly ancient varieties and clones were genotyped using microsatellite loci. The investigated 6 loci were sufficient to distinguish all cultivars. Some clones could also be separated based mostly on the variable VVS2 loci. For 17 out of the 31 investigated cultivars this is the first report on characterization of the polymorphism of the allele lengths by microsatellite markers on loci: VVS2, VVMD7, VVMD27, vrZAG62.
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Historical background and constraints of a grapevine germplasm foundation in Hajdú-Bihar county, Eastern Hungary
11-17.Views:279The historical background of Debrecen linked to viticulture and wine-making stands mainly on the lack of drinkable water, the necessity of drinkable liquid during wartime and epidemics. The special character of the city evolved together with the changing lives of citizens and the increasing trade importance of the city. Period of Turkish occupation gave impetus to the formation of the 11 vine gardens of the settlement. After the devastation of rootmite and peronospora ‘Kadarica’ and ‘Nagy burgundy’ (‘Blaufrankish’), in smaller proportion - on lower sites – ‘Cabernet’ were planted. As white varieties ‘Ezerjó’, ‘Olasz Rizling’, ‘Kövidinka’, ‘white Mustos’, in smaller proportion ‘Szlankamenka’, ‘Erdei’, ‘Szilvaner’, ‘Mézesfehér’, ‘Bakar’, ‘Veltelini’ (red), ‘Fehér burgundi’ (? white burdunder), ‘Rajnai rizling’, ‘Red Tramini’, ‘Furmint’, ‘Muscat Lunel’, ‘Járdovány’ and ‘Juh-fark’ were planted. After the Trianon treaty in 1920, 2/3rd of Hungary was cut away. Érmellék wine region was also cut in two, thus Debrecen broke away from its wine region. Legal regulations after the World War II. (1959) referred back to variety application advised in 1924 for “place suitable for good wine production, not included in any wine region”, like Debrecen listing ’Ezerjó’, ’Mézesfehér’, ’Olaszrizling’, ’Bánáti rizling’, ’Furmint’, ’Hárslevelű’, ’Kövidinka’, Kecskemét virága’, ’Piros szlankamenka’,’Pozsonyi fehér’; ’Kadarka’, ’Oportó’ and ’Kékfrankos’ (Blaufrankish). The political changes of 1990 and Hungary’s admission to the Eurepoean Union almost annihilated the wine production of Debrecen. However little gardens conserved historic varieties which could date back even to many centuries. Through a local magazine a collecting work was announced pointing to gather ancient local (Vitis vinifera conv. pontica) varieties forming a genebank, established on the experimental station of the University of Debrecen. In 2014, about 112 items were collected (accessions). As a 2nd round of the work, with a more detailed and precise work, further 81 items were put into the reservatum. The latter represent single stuck collection, whereas the first ones are to be studied az mixed items. Most notable accession names (ACENAME) of the work are: ‘Fehér gohér’, ‘Veres gohér’, ‘Fekete gohér’, ‘Kék gohér’, ‘Erdei’, ‘Ezerjó’, ‘Kűbeli’, ‘Rizling’, ‘Mézes fehér’, ‘Dinka’, ‘Madling’, ‘Bakator’ and ‘Kadarka’. Simulteneously with the strenghening and morphological description of conserved stucks genetic identification of the items is being elaborated. Database comprising FAO/IPGRI multi-crop passport descriptors and OIV Primary descriptor priority list are to be published on-line in between the development of the platform.
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A model of full bloom starting date of some white Vitis vinifera L. varieties grown in Helvecia
21-25.Views:182Grapevine bloom happens between end of May and the middle of June in Hungary. However, climate change in the past decades and the occurring weather anomalies can modi fy this date to a diverse degree. Among the weather factors, the bloom starting dates of grapevine depend mostly on temperature and relative humidity of air. There can be significant differences between North American and East Asian grapevine varieties, and of course, the early and late ripening varieties. ln this approach we investigated the starting dates of bloom between 2000 and 2004 for grapevine varieties grown in Helvecia, as well as the effectiveness of a temperature sum model. The model is based on the widely accepted cumulated heat sum concept, and the optimization was made for the least standard deviation in days as well as on the least average absolute deviation in days and on the least maximum deviation in days. The model is connected directly to a similar model for the budburst date of the same plantings (Hlaszny & ladanyi, 2009). We set the optimum lower base temperature to I 0.45 °C and the upper base temperature to 26 °C. The absolute values of the differences between the observations and the model estimations move between one and six days with an average of 1.81 days
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Legal harmonization of the Hungarian horticulture conditioned by the accession
23-28.Views:177In the EU-horticulture is treated with special care. As far as its regulation is concerned, different orders are in force to single branches. The regulation of the branch of medicinal plants is related chiefly to the processing, because the overwhelming part of the basic material is to be imported. More preoccupation falls to the ornamental plant branch, although both the production and the turnover are controlled by the market. Irrespective of this fact, the quality standards are high. Also the production and the sale relations are carefully circumscribed, with special regard to the processing procedures. In the control of the quality an important role devolves on the producers' organizations, likely in our country on the so-called TESZ-es (Cooperatives for production and sale). In this regard the domestic prescriptions took much over of the EU practices.
The structure of sales in vegetable and fruit branches is continously changing, the demands of the consumers are more and more satisfied by the super- and hypermarkets. Their marketing organizations offer almost the half of the total of quantity commodities. In consent with the processing industry they raise quite severe requirements to producers. These requirements are to be taken into account also among our circumstances. There is a similar situation valid in the viticulture and winery. Within these branches we are able to compete on good chances of adapting our regulations to those of the EU. In this area there falls also responsibility to our vine-growing communities. Particulary a watch must be kept over the reputation of our renown quality wines and the results already achieved must be protected.
The competent ministries — at first the Ministry of Agriculture and Rural Development — has already shown great flexibility to the desires in regard to the expectable legal harmonization. The legislative, registrative and controlling activities are to be continued in this mentality.
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Grape variety comparison of different stress tolerance based on the quantitative measurement of carbohydrates
37-40.Views:183The analyses of various host-pathogen relationships have established the response reaction roles of carbohydrates — especially monosaccharides — measurable in the vegetal parts of the host. Published results also provide information concerning the way various pathogens utilize carbohydrates and concerning the carbohydrates pathogens prefer out of the "selection" provided by the host plant. The role of carbohydrates in the response reactions to abiotic stress has been studied on several plant species as well — currently, too, it is an often discussed area of research. The above-mentioned results form the basis of our intention to study the connection between susceptibility to grey mould and the quantity of measurable carbohydrates in the leaves of grape varieties of various stress tolerance levels.
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Transformation of tobacco plants with virEl gene derived from Agrobacterium tumefaciens pTiA6 and its effect on crown gall tumor formation
53-56.Views:158The VirEl protein plays a key role in the transport of VirE2 protein from the bacterium to the plant cell during crown gall tumor induction by Agrobacterium. The virEl gene of A. tutnefaciens pTiA6 was cloned into the plant transformation vector pTd33 yielding pTd93virEl that was introduced into A. tuniefaciens EHA101 and used for tobacco transformation. The presence of the foreign DNA in the putative transgenic plants was confirmed by PCR analysis. Nine of the 41 transformed plants formed only small tumors following infection with the wild-type A. vitis octopine strain AB3. This property was inherited into the T1 generation. The expression of virEl gene in TI plants was demonstrated by Northern blot analysis.
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The evaluation of grape vine decline pathogens in the experimental field of the Georgikon Faculty of Agriculture in Cserszegtomaj
19-22.Views:193Vine decline causes important economic loss in viticulture, especially in longer view. Several causal pathogen were described lately, however little is known about the etiology or epidemiology of these pathogens on grapevine rootstock. It is well known that these diseases affect grafted and rooted grapevines and are not specific to any scion-rootstock combinations. Our aim was to determine what pathogens are presents in the experimental field, especially causal agents of the rootstock decline. Different grapevine rootstocks and scion varieties were tested in our trial. Isolations were made from the wood tissue and pathogenity tests were done with isolated Cylindrocarpon destructans. The possibility of infection during the propagation process was studied as well. Most commonly Cylindrocarpon sp. and Phomopsis sp. species were identified from the examined varieties. Cylindrocarpon destructans was able to spread to apical (shoot) and basal (root) direction from the point of infection with uneven speed. Callus development is not inhibited by the fungi causing the leaf symptom of the vine decline. Shoot development is reduced if unhealthy parts are grafted to each other.