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Treatments for improving tree growth, yield and fruit quality and for reducing double fruit and deep suture incidence in “Desert red” peach trees
7-19.Views:268Five years old “Desert red” peach trees budded on Nemaguard rootstock and grown in sandy soil at commercial orchard Al-Nubaria city, El-Behira Governorate-Egypt were treated with some agricultural treatments involving thinning out pruning, fruit thinning and foliar application of potassium silicate (25% S +10% K2O) at 0.1% and super grow (20% N, 20% P2O5 and 20% K2O) at 0.3% in 2014 and 2015 seasons, to study their effects on yield and fruit quality and the relationship between nutrient balance and yield of “Desert red” peach trees. Beside, testing the influence of used treatments on two physiological disorder, double fruit and deep suture %. Also, economic evaluation of different treatments was done. All obtained data were statistically analyzed using a randomized complete block design. Depending on the obtained results in this study, it could be concluded that application of thinning out pruning 35%, fruit thinning by leaving 15 cm between fruits on one-year old shoot at 20 days after full bloom and foliar application of potassium silicate which sprayed five times during each growing season at fruit set, the second fruit development stage, the beginning of the fruit color change and twice after month from harvest, most profitable treatment for peach trees grown under conditions of this investigation. This treatment gave the best vegetative growth, yield, fruit quality, higher crop value with high net income /fed. from “Desert red” peach trees, in addition, reduced the percentage of double fruit and deep suture by more than 50% in both seasons, therefore, the study recommends this treatment for “Desert red” peach growers.
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In vitro propagation of 'Echo' cultivars of Eustoma grandiflorum (Raf.) Shinn.
87-91.Views:133Eustoma grandiflorum (Raf.) Shinn. 'Echo' Fl cultivars ('Echo White', 'Echo Rose', 'Echo Blue', 'Echo Blue Picotee') were used and multiplication of shoots was evaluated on Murashige and Skoog (1962) basal medium with 11 g/1 agar-agar and 20 g/1 sucrose. To test the effect of BA different concentrations were added: 0.10, 0.25 mg/1 and a culture medium without BA. Differentiation of roots was examined on Jámbor-Benczúr and Marta (1990) basal medium with the same concentration of agar-agar and sucrose. To examine the effect on rooting, various concentrations of NAA were used: 0.5, 1.0, 2.0, 3.0 mg/l. The pH was adjusted to 5.6 in every case using KOH. We studied the after-effect of different concentrations of BA during the acclimatisation. During the multiplication, the cultivar 'Echo White' formed the most shoots and the smallest leaves on the medium with 0.10 mg/1 BA. Fortunately, in the case of this cultivar, the number of shoots was reduced and the length of leaves was increased succesfully on the medium without BA. The other three cultivars developed the longest leaves on the medium containing 0.10 mg/1 BA. Sometimes not only shoot regeneration but spontaneous rooting was observed during the multiplication. Examining the rooting, the highest percent of roots was found on the medium with 1.0 mg/1 NAA, and the cultivar 'Echo Rose' formed the most roots on this medium. Higher concentration (2.0 and 3.0 mg/1) of NAA already reduced the number of roots in all of the cultivars. During the acclimatisation, the percentage of survival was 76.3% and the tallest plants with the longest leaves were found on the multiplication medium with 0.25 mg/1 BA. 'Echo Blue Picotee' gave the best results with the tallest pieces and longest leaves on this medium.
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A complex system for the production of pathogen-free grapevine propagating material
59-62.Views:207The use of pathogen-free planting stock for new vineyard establishment is a key component in the maintenance and expansion of vine and quality table grape production. The success of the necessary changes in the structure of the grape industry is forced by the globalization process, the climate change, the rediscovery of autochton varieties as well as breeding of new tolerant and resistant varieties. The renewal of vineyards largely depend on the availability of planting stocks. Serbia and Hungary found a common interest in establishing pathogen-free stock materials from newly breed resistant varieties and clonal selections of varieties which are traditional in the Serbian-Hungarian border area. During a cross-border cooperation program a complex system for the production of pathogen-free grapevine propagating material was established. Using heat therapy, in vitro shoot tip culture and traditional and molecular diagnostic techniques new pathogen-free stock materials were established from 26 varieties. They have been or will be tested for the presence of most important grapevine viruses, phytoplasmas, as well as bacterial and fungal pathogens. The complex system applying green grafting for indexing on grapevine indicators can shorten the duration of the procedure from 4 years to two-three years.
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Hungaricum as a quality of fruits and fruit products
71-81.Views:183The territory of the Hungarian state is largely suitable for the purpose of growing fruits of the temperate zone species. During the next decennia, the annual volume of Hungarian fruit production is expected to be around 1.1-1.3 million tons, from which some 15% is considered to be a produce of Hungary or "Hungaricum" (90 thousand tons of sour cherry, 50 thousand tons of apricot, 20 thousand tons of raspberry, 10 thousand tons of walnut). These fruits symbolise the country's special quality, which are worth to catch the interest the foreign consumers.
The category of Hungaricum involves almost exclusively varieties of Hungarian origin as sour cherries, apricots, raspberries and walnuts, and they are representing outstanding qualities on the international markets.
As for the fruit products the fruit brandies are eligible to be "Hungaricum" and are called exclusively "Pálinka". The Pálinka, provided to be distinguished with a geographic mark and will be competitive on the world market. Smaller quantities, though significant produce is represented by the deep frozen raspberry.
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Morphological, physiological features and differences of Vriesea splendens ’Fire’ plants during in vitro multiplication and rooting
Views:114During in vitro multiplication and rooting of Vriesea splendens ’Fire’, 0.1, 0.2, 0.4 and 0.8 mg l-1 benzyladenine (BAP), benzyladenine-riboside (BAPR), kinetin (KIN), meta-topoline (MT), indole-butyric acid (IBA) and naphthalene-acetic acid (NAA) were added to basal Murashige and Skoog (1962) MS medium. As compared to the hormone-free control, plants developed significantly more shoots on medium supplemented with almost all cytokinins (excepting KIN), especially BAP resulted the highest multiplication up to almost 26 shoots. Enhancement of cytokinin concentrations increased shoot number (and in case of BAP, peroxidase activity) but decreased plant height and rooting parameters. Regarding root production, both auxins were definitely beneficial (0.2 mg l-1 NAA resulted more than 7.5 roots and higher auxin concentrations efficiently stimulate root elongation); however, KIN had similar effects. After a three-month duration time of acclimatization, we observed that plants which were previously cultured on medium containing certain cytokinins (KIN in all doses and 0.1 mg l-1 MT) or both auxins had greater survival, moreover, as negative after-effect, higher cytokinin concentrations reduced the number of survived specimens.
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Rootstock effects on fruit drop and quality of 'Arlet' apples
69-75.Views:200The aim of this study is to investigate the effect of different growth inducing rootstocks on fruit drop of apple. This research was conducted for 3 years at Nagykutas, Western Hungary on apple cv. 'Arlet'. For the experiments, 3 different growth inducing rootstocks were choosen: M.9 (weak), MM.106 (moderate) and crabapple seedling (strong). There were 3 fruit shedding periods on the trees grafted M.9 and MM.106 rootstocks, but in the case of crabapple seedling, only 2 were found. The measure of fruit drop was closely related to seed count of fruits; seed number was the lowest, fruit drop was the highest. The lowest seed number was counted in fruits from trees on crabapple seedling. Seasonal changes of leaf:fruit ratio mainly depended on shoot growth and fruit drop. The rise of the curve of leaf:fruit ratio was very important during the first phase of fruit development, in especially at the end of June and in the beginning of July. The cause of this is that first and second periods of fruit drop appeared during this term. The rise of the curve was important in the beginning of June and the end of July on crabapple seedling. Decreasing tendency of quality parameters was found of fruits from trees on M.9, MM.106 and seedling rootstocks, except flesh firmness which was the highest in fruits from trees on crabapple seedling.
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Comparative study of heirloom tomato varieties
31-33.Views:189The study examined the health status, growth type, berry morphology, berry quality, and gastronomical value of 60 heirloom tomato lines. In the middle of June, the level of virus infection was medium in the plant stock according to the assessments. 21% of the lines did not show symptoms of virus infection. During the vegetation period, the major fungal diseases were Alternaria by the middle of August and Phytophthora from the beginning of September. In spite of the fact that no fungicides were applied, 13% of the lines were healthy and 36% showed medium infection at the end of August. Half of the lines had a strong, 23% of the lines had a medium growth vigour. The majority (74%) of the lines showed strong or medium lateral shoot growth and only 7% of them proved to be less prone to branching. In spite of the rapid growth, 20% of the lines had a weak main stem, the majority of the lines had a main stem medium strength. The different shapes occurred in different colours, in addition to the traditional red colour (53%), there were orange, yellow, wine-red (blackish), lilac, green, almost white or multicoloured varieties. Unfortunately, most of the lines were prone to longitudinal or circular cracking of the berries. Based on the first experiences, all tested variety types had more favourable utilization characteristics then the traditional tomatoes. Varieties with a thick flesh and harmonic acid/sugar ratio such as some of the ox-heart varieties are especially valuable. For drying, the elongated types proved to be the best. The hollow tomato is the most special type which can be utilized most diversely. It is especially suitable for making white tomato soup and, “Concasse" due to its easily removable placenta rich in jelly materials and its thick flesh. In addition, it can be used for preparing stuffed tomato.
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Inheritance of fruit colour and of shoot's pigmentation in the case of interspecific raspberry hybrids
33-36.Views:105In our research, fruit colour and the shoot's red pigmentation were evaluated in the hybrids of Rubus idaeus and Rubus parvifolius crossings. Y and Ys genes beside the T genes determine the fruit colour of interspecific hybrids, which is characteristic for raspberry. For the explanation of the significantly higher results of segregation then expected at the yellow fruit colour hybrids, we have supposed the presence of a second yellow gene (Y2). In the yellow colour, a lot of different shade colours can be identified from light yellow to the apricot colour. In the regulation of the production of yellow and red colour, several other genes can participate also. Identification of these genes would require more additional research. The C gene determines the shoot colour of raspberry and in the case of wild raspberries we have revealed the role of a dominant Pr gene. The Y and Pr genes are descended linked. The value of crossing over is approximately 15%. The anthocyanin production inhibitory effect of the Y gene extends only for fruit. At the shoots of yellow fruit plants, strong anthocyanin production was observed.
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Bacterial diseases of grapevine
45-49.Views:368Grapevines are affected by three major bacterial diseases worldwide, such as bacterial blight (Xylophilus ampelinus), Pierce’s disease (Xylella fastidiosa) and crown gall (Agrobacterium vitis). These bacteria grow in the vascular system of their host, thus they invade and colonize the whole plant, independently on symptom development. Latently infected propagating material is a major factor in their spreading. Therefore the use of bacteria-free planting stock has a basic importance in viticulture. Today several innovative diagnostic methods, mostly based on polymerase chain reaction, are available to detect and identify bacterial pathogens of grapevines. For production of bacteria-free plants, the use hot water treatment followed by establishment of in vitro shoot tip cultures is proposed.
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The effects of growth regulators in proliferation of Sorbus redliana 'Burokvölgy'
77-83.Views:191The Hungarian cultivar Sorbus redliana 'Burokvölgy' was proliferated on Murashige and Skoog (MS, 1962) medium with half-strength macroelements and 100 mg/1 meso-inositol, 20 g/1 sucrose, 11 g/1 agar-agar. Different combinations of kinetin (KIN), metatopolin (mT), benzyladenine (BA), benzyladenine-ribosid (BAR) and indolebutiric acid (IBA) were tested, and pH was adjusted to 5.6 every case using KOH. The cultures were incubated at 20-24 °C in 8/16 hours dark/light photoperiod for 50-52 days. The main aim of our research was to find the optimal growth regulator and its optimum concentration. Purthermore, to determine the chlorophyll contents of the in vitro propagated plants' leaves. During the proliferation, the highest number of shoots were observed in the case of using BA + IBA, and on the medium containing 0.75 mg/I BA + 0.05 mg/1 IBA 8.93 shoots were found. The addition of KIN + IBA decreased the number of shoots and increased the sizes of leaves — the widest (11.2 mm) and longest (17.8 mm) leaves were obtained on the medium containing 1.00 mg/I KIN + 0.05 mg/1 IBA. The longest shoots (36.46 mm) were found in the case of applying 0.75 mg/1 BAR + 0.05 mg/I IBA. The BA + KIN + IBA combination resulted the shortest shoots. Sometimes not only shoot regeneration but spontaneous rooting was observed during the multiplication. The highest chlorophyll content (1.569 mg/g total chlorophyll, 1.132 mg/g chlorophyll-a, 0.437 mg/g chlorophyll-b) was obtained in the presence of 1.0 mg/I KIN + 0.05 mg/1 IBA.
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Water use of young apple trees related to leaf area development
61-63.Views:132Present paper describes an investigation concerning seasonal water use and foliage area development of apple trees. Sap flow velocity was measured in the trunks of five years old apple trees cv. `Florina'/M.26 by a thermal dissipation (Granier) method from 20th of May to the end of September in 1998. The development of foliage area was estimated by a method including leaf area measurements, recording of leaves and shoot length. The foliage area reached to 70% of the maximum yearly value at beginning of June. The remaining 30% developed to the end of August. The leaf area specific water use was considerable higher in June and July, than in second part of summer. The trends of ET-FAO and water use curves differed mostly in the late season: the ET-FAO curve falls quite in September compared to August, whereas the value of water use was a similar as in August. This insensitivity of ET-FAO in this period may be a great disadvantage while using the Penman-Monteith equation in irrigation scheduling of apple.
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Fire blight in Hungary between 1996 and 2003
67-70.Views:109Shoot blight of pome fruits caused by Erwinia amylovora, i.e. fire blight, is present in numerous countries of Europe. The disease must have entered into Hungary in the middle of the 1990's and it was first noted and, respectively, identified in 1996 (Bacs-Kiskun county). The losses caused by the pathogen appeared — in orchards and scattered sites of production — in four counties, namely Bacs-Kiskun, Baranya, Bekes and Csongrad at the beginning. From June 1996, a process of eliminating infected parts started in the course of a large action performed under the control of the Department of Plant Protection and Agro-Environmental Economy of the Ministry of Agriculture, under the direction of the plant protection inspectors of the then existing Stations of Plant Health and Soil Conservation. The 'operation' against the disease commenced by cutting back out the infected parts of the canopy and, grubbing them out, respectively. As for the spread of the pathogen (1996-1998) it could be observed that the disease entered into Hungary from the south, south-east and then it also spread into the middle part of the country. As a result of adequate official action and efforts as well as of adequate chemical and antibiotic treatments, moreover because of the introduction of more modern technologies of plant cultivation and those of plant protection it can be reported on that the pathogen hardly appears or does not occur at all on the northern, north-western part of the country. The infection also appears mainly on the parts east of the Danube. Cultivars less susceptible or non-susceptible to the disease are planted in recently established orchards what is also a considerable factor in respect of preventing spread of the pathogen.
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Cost-effective plantlet production and wintering method of virginia fanpetals (Sida hermaphrodita L. Rusby)
137-141.Views:199The main goal of this research was to work out programmable, cost-effective and industrial scale technologies of mass propagation from the seeds of rootstock nurseries of undomesticated American populations of Sidahermaphrodita. During our previou`s seed treatment experiments, it was concluded that around 60% of the Virginia fanpetalsseeds collected during the four cropyears can be considered as high quality, infection-free, normally imbibing and germinating seeds (Kurucz et al., 2013a,b). The experiments performed with the nurse-in-tray method developed by us showed that the summer-autumn nurse-in-tray plantlet production and unprotected wintering of Virginia fanpetals with properly pre-treated and fractioned seeds is a promising new method. No weeds appear between the plants, but only on the side of the cases during plantlet production. The investment cost of the method is minimal. There are no heating costs and this phytotechnique can be easily and properly mechanised. Plantlet production can be performed near the large-scale plots. After exploring the root and shoot system, it was concluded that the nurse-in-tray method is suitable for producing plantlets with hardened and strong roots. Scheduled plantlets can be produced in an industrial scale volume by the time of early spring (March) plantlet planting. The excavateof plantlets can be flexibly adjusted to the needs; they may even grow in the plantlet cases for a whole year. We think that these innovative plantlet production and wintering methods which are suitable for large-scale use will make Virginia fanpetals a proper feedstock for the constant supply of the Biomass Supply Chain both in Hungary and in European countries which are in the same climate zone. The comparative analysis of the costs of this procedure calls for further research.
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Anatomical relations of the leaves in strawberry
81-84.Views:165In the present study histology of the leaves of strawberry (Fragaria ananassa Duch.) variety Elsanta was the objective, which has been performed with the beginning of seedling stage, cotyledons, primary leaves and later true leaves, first cataphyll of the runner shoot as well as the bracteoles of the inflorescence. Structures of the leaf blade, the upper and lower epidermis, the petiole have been also observed. The leaf blade of cotyledons already contains a typical palisade as well as spongy parenchyma tissues, i.e. being bifacial showing a structure similar to that of the true leaf. However, the petiole displays differences from the true leaf. There are a narrow (4-5 layer) primary cortex and a tiny central cylinder. Primary leaves bear already hairs on the adaxial surface and the transporting tissue-bundles are recognised in cross sections having a "V" shape. The first true leaf composed by three leaflets is of a simple structure showing characters reminding of cotyledons and primary leaves. Leaves of intermediate size continue to grow, whereas their inner anatomy changes dramatically. In the central region of the leaflets, near to the main vein, a second palisade parenchyma appears, further on, transporting tissue bundles are branching in the petiole. Collenchyma tissues enhance the stiffness and elasticity of the petiole. Older true leaves develop thick collenchyma tissues around the transporting bundles being represented by increasing numbers. The doubled palisade parenchyma layers of the leaf blades are generally observed. The cataphylls of the runners have a more simple structure, their mesophyll is homogenous, no palisade parenchyma appears. It is evident that leaves grown at successive developmental stages are different not only in their morphological but also anatomical structure. There is a gradual change according to the developmental stage of the leaves.
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Anatomical study of the bud union in „Chip" and „T" budded 'Jonagold' apple trees on MM 106 rootstock
27-29.Views:357The traditional methods for vegetative propagation of apple and its varieties are the T-budding, and the winter grafting, but this latter way is a difficult and expensive procedure.
In our experiment carried out in the Fruit Tree Nursery Soroksár, the healing process of chip- and T-budded apple trees 'Jonagold' on MM 106 rootstock was studied.
The budding (T- and Chip-) was made in the first week of August, samples for microscope examination were taken monthly after this time until leaf fall.
The investigated part of plants was made soft with 48 % HF (hydrogenfluoride), then cross and longitudinal section were made and examined by microscope.
Based on analysis of microscope pictures in case of Chip-budding, it was established, that development had started quickly after budding on the rootstock and scion too. But the callus originated almost entirely from the rootstock tissue as new parenchyma cells fills the gap between the two components of graft (scion and stock), becoming interlocked and allowing for some passage of water and nutrients between the stock and the scion. This quantity of callus in case of T budding was under the scion buds larger, than the Chip-budded unions, where the thickness of callus mass is uniformly thick round the chip. The large mass of callus pushes the scion bud outwards from the shoot axis, which later results in a larger shoot-curvature above the bud union.
Following this process on the Chip-budding it can be observed also, that a continuity of the cambium is established between bud and rootstock. Then the newly formed cambium started typical cambial activity, forming new xylem and phloem.
Later the callus begins to lignify, and it is completed within about 3 months after budding.
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Effects of crop load on tree water use in apple (Malus x domestica Borkh.)
29-31.Views:118Sap flow rate measurements were carried out during two consecutive ('on' and 'off') years in an apple orchard cv. 'Florina' M.26 to analyse the effects of various crop load on tree water use. Sap flow rate was measured by thermal dissipation method in trunks of nine trees from June to the harvest. Crop load was between 0.2-9.0 fruits • cm -2 of trunk cross section area (equal to 0.5-35.0 fruits•m-2 of leaf area), trunk diameter varied between 4.7-8.7 cm.
Total leaf area was estimated by leaf counting or using shoot girth and leaf area relationship. In both years, fruit growth rate was determined by measuring diameter of 280 marked fruits with 7 days frequency. Fruit volume calculated as a function of fruit diameter based on a previously determined relationship.
Total leaf area of trees ranged from 4.4 to 19.5 m2 and it was closely related to trunk cross section area. At high crop load the fruit growth rate peaked in August with 0.51.tree-l.day-1 and the leaf area specific total fruit volume reached 61 m2 before the harvest.
There was a linear relationship between total leaf area and daily water use, while the leaf area specific water use was influenced by crop load rate. The relationship was described by piecewise linear regression with the breakpoint at crop load rate of 12 fruits•m2 of leaf area. At low crop load the slope of the fitted regression line was less than at high crop load rate.
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In vitro plant regeneration from immature embryo axis and cotyledons of common bean (Phaseolus vulgaris L.)
93-97.Views:127Phaseolus vulgaris L. is the most important economic species within the genus Phaseolus. It is grown in all parts of the world. Genetic improvement by conventional breeding has met considerable success, although production of hybrids between species within the genus has been limited due to sexual incompatibility. Recent advances in tissue culture have offered the opportunity to produce cultivars, which could not be obtained by conventional breeding methods. The use of tissue culture and genetic engineering is viewed as a logical approach to improve bean production. Gene transfer techniques will have a great impact on legumes. Although the concept of cell totipotency is widely proved, in vitro morphogenesis has not yet been achieved for a large number of cultivated beans. Regeneration protocols are strongly influenced by the genotype. In tissue and cell culture of beans, the factors controlling shoot morphogenesis and somatic embryogenesis are still unknown. The reported data suggest a possible way for future research.
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Effects of different rootstocks on the morphologic parameters of watermelon grafts during transplant production
69-73.Views:165Before Hungary joined the EU watermelon production was a key sector of the Hungarian vegetable industry. Its production area shrank by nearly 60 % since 2004, it is only 5000 ha today. Stable and reliable market failed to evolve in the last years and extreme weather events occurred more frequently. An unfavourable growing season can notably affect the production volume of the next season. Information gathered in the last years support the assumption, that suitable stocks can help eliminate the issues caused by extreme weather or short-term crop rotation. Choosing the right grafted or non-grafted transplant is not enough any more, growers also have to select stock. In fruit and vine production choosing the stock, which is the most suitable for the technology is evident and this approach should be widely adapted by watermelon growers as well. In this research we investigated the growth parameters of different watermelon stocks and their effect on the scion during the transplant production. Measurements were carried out on seven different stock-scion combinations of the scion ’Topgun F1’ hybrid. Two of the stocks were interspecific (Cucurbita maxima × Cucurbita moschata) and five stocks were Lagenaria-type (Lagenaria siceraria) stocks. We made and recorded observations of the transplants’ length (cm), diameter of the stock and scion (mm), number of leaves (piece), root length (cm), root mass (g) and shoot mass (g). We concluded that early pricking out of transplants with interspecific stocks is successful due to their hardiness and vigorous growth. The effect of the ’Shintosa camelforce F1’ interspecific stock was outstanding. Furthermore, the results confirmed the practical observation that the root system of interspecific stocks grow vertically more allowing them to take up water and nutrients from lower soil layers in poor sandy soils. From the Lagenaria group the root system of the ’DG-01 F1’ was similar to the interspecific stocks’. It can be grown even in sandy soil, however other Lagenaria-type stocks are more suitable for production in hard soils with nutrient supply because of their compact root system. This study can serve as a basis for further research in the topic that can conduce to site- and technology-specific stock selection.
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In vitro multiplication and hardening of grapevine plants in aeriated media
15-18.Views:207In vitro cultures have widely been used in horticulture for rapid multiplication of new varieties and clones as well as to produce pathogen-free stock material. To improve efficient hardening and transfer in vitro grown grapevine plants were multiplied by cutting them into single-node internodes with the whole leaf. Microcuttings including the shoot tips were rooted in granulated perlite moisted with tapwater under sterile conditions. After 2-3 weeks the rooted microcuttings were supplied by nutrients and hardened by gradual opening and finally by complete removal of the lids of jars or plastic boxes used for growth. Using this method microcuttings of Vitis vinifera cvs. „Chardonnay", „Cabernet franc", „Riesling" and „Sauvignon blanc" and the rootstock varieties Vitis riparia x Vitis cinerea cv. „Barrier" and Vitis berlandieri x Vitis rupestris cv. „Richter 110" formed new roots and shoots and 100% of the tested plants survived the acclimatization procedure. Similar results were obtained when perlite was replaced with rockwool-, or pit-pot blocks. This method may highly increase the efficiency of producing pathogen-free propagating material and new transgenic lines.
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Production of transgenic carnation with antisense ACS (1-aminocyclopropane44-carboxy late synthase) gene
104-107.Views:167Dianthus chinensis and Dianthus caryophyllus varieties were tested for shoot regeneration from leaf and petal explants and transformed with Agrobacterium tuniefaciens strains (EHA 105 and LBA 4404) harbouring an apple derived ACS cDNA in antisense orientation in order to reduce ethylene production and influence the ethylene dependant traits in carnation. After transformation regenerating shoots were selected on MS medium containing 50-75-100-125-150 mg/1 kanamycin and supplemented with 1 mg/1 BA, 0.2 mg/1 NAA. Transgene integration was proved by PCR analysis with npt II spcific primers followed by Southern hybridisation of DNA isolated from green shoots on medium containing 150 mg/1 kanamycin. Several putative transformants were subjected to RT-PCR in order to examine the npt 11 expression at mRNA level. Both the transformant and the non-transformant plants were potted into glasshouse to observe the effect of changed ethylene production on flowering time, petal senescence and vase life.
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Effect of different non-chemical treatment methods on organic seed
63-65.Views:114The importance of ecological farming in Hungary is increasing. The utilisation of organic seed is regulated by national law. In our experiment the effect of two plant conditioning substantial: Biokál and Biomit Plussz were tested on the germination of seed. Our test plant species was garden pea (Pisum sativum L.), the most important vegetable in Hungary. The experiment was carried out in the germination laboratories of the National Institute for Agricultural Quality Control after international rules. The germination, the length of root and shoot were measured. 4 hours soaking of pea seeds in 30% solution of Biokál gave the best result. Our results could help to make the field emergence of organic seed faster and safer.
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Application of the Jerusalem artichoke (Helianthus tuberosus L.), as a plant origin medium additive, during the micropropogation of Ada keiliana
61-64.Views:184A procedure for in vitro propagation of Ada keiliana seedlings are suited for acclimatization, was worked out. M medium was supplemented, with Jerusalem artichoke, as plant origin complex additive. The apply of JAD (1,5g/flask) gave the best response, considering the shoot (29 mm), and the root development (24,9) mm) too. The plantlets with satisfying growth (25-30 mm, 4-5 roots) were transferred in small pine bark: Novobalt peat: coconut fibres: perlit (2:3:1:1) mix, among greenhouse circumstances.
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Foliar application of zinc and its effect on greenhouse grown cucumber
79-82.Views:159The experiment was conducted to examine the effect of the foliar application of zinc on yield and crop quality and on fruit mineral composition of cucumber plants (Cucumis sativus cv. Mustang) which were grown in peat in containers under unheated conditions and were not showing visible signs of zinc deficiency.
In the trial the following 3 treatments were set up in 4 replications: Znl = 0.35 g/litre Zn, as foliar fertilizer; Zn2 = 0.7 g/litre Zn, as foliar fertilizer, control = no foliar fertilization. Foliar fertilization was applied 5 times with 10 day intervals. After their planting out the plants were fertigated daily with water soluble complex fertilisers. Fruits were harvested twice a week, 16 times in all, and were divided into three quality classes (class I, class II and substandard). Shoot length of the plants (plant height) was measured on 3 occasions. Zinc content of the fruits and leaves was analysed at two times.
From the results of the trials it can be concluded that the 0.35 g/litre Zn (0.35 mg/ml) foliar fertilisation had beneficial effect on cucumber both in terms of yield and quality. Under the conditions of the experiment (daily fertigation through drip irrigation) the effect of a more concentrated foliar application of zinc seemed less beneficial.
The zinc content of the fruits showed no evident increase in response to foliar fertilization, while a significant increase was seen in the leaves, particularly with the more concentrated Zn treatment. This indicates that in the case of cucumber zinc, through its assimilation in vegetative parts, has an indirect effect on fruit development.
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Effect of hail protecting nets on the quality of apples
77-80.Views:237Today, successful fruit growing depends largely on the security of production. Among the technological elements of a secure growing system, the use of hail protection nets serves special attention. Components of security are the regularity, conspicuous large yields with excellent quality, which determine the prices and profitability as well (Szabó et al., 2010). In the European Union, most of the apple growing countries produce higher yields by 10-20% than Hungary. The conditions of better yields are partly due to a more favourable ecological milieu, partly to more developed technologies and serious discipline of the operations. Our own competitiveness could be developed first of all in those moments. As a consequence of global climatic changes, excesses of weather, i.e. hail-storms became more frequent. Different methods of protection against hail are developed abroad but also in Hungary. The examination of effects of the hail protection nets compared with the check without nets has been the purpose of our experiments. The growing system was the intensive one with high planting densities. Fruits of the two subsequent years have been compared as grown with and without hail protective nets. It turned out that in one season when shoot growth was stimulated, the shadowing effect of the net increased in addition the growth and at the same time braked the differentiation of flower buds, which exerted negative effects on yield of the next year. Let alone this phenomenon, the quality of fruits was not influenced by the hail protection net.
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In vitro regeneration from cotyledons of watermelon
96-98.Views:163Cotyledonary segments of five different genotypes of watermelon were used to induce organogenesis. Five different hormone combinations were applied to enhance the induction of shoot formation on the surface of the segments. The phases of organogenesis were followed with light and scanning electron microscope. Shoots were obtained after four weeks, then the shoots were transferred to hormone free medium for root induction.
This method of regeneration can be applied in transformation experiments. GUS histochemical assay was made to check the expected success of using Agrobacterium for the transformation.