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• Impact of foliar fungi on dogroses

  23-30.

  C. S. Schwer

  U. Carlson-Nilsson

  Uggla M.

  G. Werlemark

  H. Nybom

  Views:

  185

  Wild roses of the section Caninae, commonly known as dogroses, have been described as more disease tolerant than ornamental roses and could therefore become valuable for breeding improved rose cultivars. Two fields with dogroses, one with plants obtained by open pollination in wild populations, and one with plants obtained from intra- and interspecific crosses, were evaluated for blackspot, powdery mildew, rust and leafspots in the autumn of 2005. Symptoms of the different fungi on different dogrose species were carefully evaluated in a microscope and documented by photography. Interestingly, almost no symptoms of powdery mildew were found in either field, although the fungus infected wild roses of a different section in a field closeby. Surprisingly few symptoms were found also of blackspot, and they differed considerably from those found on ornamental cultivars, indicating a lower susceptibility in dogroses. The most important fungal disease in 2005 was rust, followed by leafspot symptoms. The latter were apparently caused by Sphaceloma rosarum and Septoria rosae which can be properly discriminated only in a microscope. The investigated dogrose species and their progeny groups varied significantly in disease susceptibility and in the appearance of encountered symptoms but there was no evidence of major resistance genes, except possibly in Rosa rubiginosa which did not show any symptoms of Septoria. In 2006, a subset of the plant material in Field 1 was evaluated to check for consistency between the years. Leafspots had overtaken rust as the most important disease but results were otherwise very similar to those of 2005.

• Reactions of different plant organs of pear cultivars to Erwinia amylovora infection

  17-21.

  K. Honty

  Z. Boldog

  M. Göndör

  J. Papp

  K. Kása

  M. Hevesi

  Views:

  123

  Research project has been initiated in 1999 with the aim of evaluating the degree of susceptibility/resistance of pear cultivars grown in Hungary to fire blight disease caused by Erwinia amylovora. The recently selected promising cultivars were also examined. Inoculation experiments were conducted in controlled greenhouse conditions because of quarantine regulations in Hungary. Following the disease process, development of symptoms of plant organs (shoots, flower parts, fruits) was observed. Suspension of two E. amylovora strains (Ea 21, Ea 23) isolated from pear was used in a mixture (5x10⁸ cells x m1^-1) for the inoculation. Twenty-six pear cultivars were examined and grouped into four categories: low susceptibility, moderately susceptible, susceptible and very susceptible. Most of the cultivars were susceptible or very susceptible while some promising 'Eldorado', 'Harrow Delight' and `Hosui' showed low susceptibility.

• Comparative analysis of apricot cultivars based on their ecological and biological indicators

  35-50.

  D. Surányi

  Views:

  223

  The herbaceous plants organic characterize Ellenberg et al. worked out (1991), well-use system, which is updated with herbaceous and woody plant in the Hungarian flora species, so Soó (1964-1985), Zólyomi et al. (1967), Précsényi (1986) and Simon (1988) also addressed by different aspects of this problem circuits. The author is the first extended-Borhidi –Ellenberg’s system of wild fruit species (Surányi 2000, 2006) and cultivated of fruit (Surányi 2014) as well. Additional considerations there were aspects of the study of fruit varieties, these biological indicators following open pollination, frost tolerance, resistance of Sharka virus and disease   susceptibility for. Firstly, we introduced a system for improving it a plum species and cultivars (Surányi 2015). In this case we used the new system among species and varieties of apricots, because diversity was able to express significantly. Especially the SB, WB, NB, and the relative biological value figures showed the variety. RB (reaction figures) fluctuated only slightly among the 463 varieties, but the dynamic difference between the 11’s was an indicator for the characterization of apricots. If the comparison performed plum and apricot variety’s level anyway justified the use of 11 kinds of organic and biological indicators.

• Exploring and preserving old apple cultivars of the Carpathian basin

  9-13.

  M. Tóth

  Views:

  170

  In a few scattered orchards of the Carpathian basin one can still find our most common historical apple cultivars and certain traces of traditional cultivation. We consider it an important part of the Department's breeding work to explore old apple cultivars which can be used as parent partners and sources of resistance, to identify them pomologically as well as to examine the production and marketing value of old cultivars recommended for cultivation in ecological production and in gardens. This paper documents the results of expedition and collecting work carried out in three regions of the Carpathian basin (foot of the Carpathian Mountains, Transylvania, Aggtelek karst area), and also the saving of old Hungarian apple cultivars preserved until present in the English National Fruit Collection. A total of more than 100 old apple cultivars and variations, as well as 13 valuable genotypes were collected during our explorations in the above mentioned four areas. A further aim of our work is to contribute to the protection of still savable archaic fruit production relict= areas and to the preservation of cultivars in the certain regions, and also to promote the recognition of the aesthetic landscape value of old apple trees and endemic orchards of old cultivars. Our good co-operating partners in this work are Aggtelek National Park, Farmer Club of Visk and the consultation centre of Hungarian horticulturist training beyond the border in Nyárádszereda.

• Role of hydrogen peroxide and Pharmaplant-turbo against cucumber powdery mildew fungus under organic and inorganic production

  39-44.

  Y. M. Hafez

  Y. A. Bayoumi

  Z. Pap

  N. Kappel

  Views:

  412

  Cucumber leaves have been sprayed with a solution of hydrogen peroxide (H₂0₂) or Pharmaplant-turbo combined with organic or inorganic fertilizers under plasic house. Under the influence of H₂0₂, leaves exhibited resistant against Podoshaera fusca fungus, the causal agent of cucumber powdery mildew. H₂0₂ (15 mM) was able to decrease the disease severity from 90.4% to 12% in two experiments conducted in two seasons. Pharmaplant-turbo (Turbo) is new chemical compound and used as an antifungal compound. Turbo in 1 ml/L was able to decrease the disease severity from 90.4% to 11.5% in the both experiments as well. Both of H₂0₂ and Turbo were combined with organic treatment (compost + compost tea + seaweed extracts) which showed significant effect against cucumber powdery mildew fungus and strongly suppressed it as compared to control leaves. Organic treatment produced higher vegetative growth characters and greater early and total yields as compared to inorganic treatment, also organic fruits produced the lower nitrate content and the higher ascorbic acid content as compared to inorganic fruits. Our study have indicated that, H₂0₂ and Turbo combined with organic fertilizers play a role in the resistance of cucumber against powdery mildew by decreasing the disease severity. We suggest to give more attention to the direct application of H₂0₂ in low concentration and Turbo against powdery mildew diseases and other plant diseases.

• Field trials with non-bactericide products to control fire blight in apple orchards

  55-60.

  T. Bubán

  P. Sallai

  J. Deme

  Views:

  132

  Recently, novel strategies and chemical agents for prophylactic protection against the bacterial (Erwinia amylovora) disease fire blight are being sought. Resistance-inducing compounds, such as prohexadione-Ca represent promising alternatives. Prohexadione-Ca is the active ingredient of the bioregulator Regalis, currently being introduced in several European countries and overseas. Another product used in this study was Biomit Plussz, a leaf fertiliser providing harmonic supply of nutrient elements, the complete supply is assumed to improve the tolerance against diseases.

  Treatments' effects of both of these products were compared to the effectiveness of treatments with antibiotics repeated twice, three or four times a season.

  In the years of 2001 and 2002, the effectiveness of both Regalis and Biomit Plussz in reduction of incidence of shoot blight was similar, or proved to be superior to the check treatments consisting of repeated sprayings of antibiotics. Last year (2003) treatments of streptomycin resulted — although within the same magnitude — in a somewhat better control of shoot blight than sprayings with the other compounds.

  As regards severity of blossom blight, inconsistent results were recorded concerning both Regalis and Biomit Plussz.

  In general, prohexadione-Ca is less efficient for controlling flower infection by E. amylovora as compared to shoot infections, since successful prophylactic treatments are difficult to carry out early in the season. The highest effectiveness in fire blight management can, therefore, be achieved by using prohexadione-Ca (as preventive protection) in combination with streptomycin or other suitable antibiotics (as curative protection).

• Biological performance of grape varieties in Eastern Hungary

  31-35.

  N. Rakonczás

  Views:

  201

  Eurasian and interspecific grape varieties and candidates were inspected for biological productivity in the variety collection of the University of Debrecen at the Horticultural Experimental Station in Pallag. In this paper, data are reported on yields (kg/stock), cane production (kg/ stock) and use-up index of wood yield of each cultivar in the experimental years 2011-2012. From the range of Eurasian (Vitis vinifera L.) white wine-grape qualified cultivars and candidates ’Ezerfürtû’, ’Generosa’, ’Jubileum 75’, ’Müller Thurgau’, ’Zöld veltelíni’,’B-11’, ’CSFT-92’, ’Pintes’, from the range of Eurasian (Vitis vinifera L.) red wine-grape qualified cultivars and candidates ’Kármin’, ’Pinot noir’, ’Alicante Bouschet’ and ’Rubintos’ were highlighted. Promising data of interspecific ’Aletta’, ’Csillám’, ’Kunleány’, ’Orpheus’, ’Refrén’, ’Taurus’, ’Viktória gyöngye’, ’Alföld 100’, ’Reform’, ’RF38/32’, ’Toldi’ white, and ’Dunagyöngye’ ’Pannon frankos’ red wine-grape cultivars and candidates were emphasized. Published data refer only to the biological performance of the cultivars. The thorough evaluation of varieties needs respect to data on resistance to fungal disease and climatic extremities, and other characteristics concerning production technology and oenological parameters.

• Freeze Susceptibility of Fruit Buds in 67 Apple cultivars in Hungary

  29-35.

  M. G. Tóth

  Views:

  122

  Frost damage is one of the most important risks of apple production. Outstanding importance has been attributed to the frost resistance of flower-buds as decisive sites of fruit production. Browning of plants parts and tissues exposed to natural weather adversities are considered as effects of frost. In Hungary, frost damage on flower buds of both the market and new cultivars has not been assessed earlier. Observations referring to the consequences of frost damages of over four critical years, marked by their peculiar winter and spring frost hazards. Parallel observations have been made in four sites of the growing area in the Great-Plain region. 67 apple cultivars have been assessed. Each cultivar was represented by 3 trees, which were sampled at about 1-1.5 m height over ground, where the buds or inflorescences were picked for the purpose to assess the injury. The buds and flowers are cut longitudinally and rated visually according to the extent of browning of the organs and tissues. Susceptibility of different organs of the flower (pistils and anthers) were rated separately. According to our results, most resistant to spring frosts are the following cultivars: 'Gloster', 'Granny Smith' and appreciable tolerance is attributed in 'Gala' and 'Jonathan' with derivatives. Preliminary results that among the scab resistant cultivars, `Baujade', 'Rewena', 'Liberty', `Resi' and 'Renora' are rather frost resistant. Information, lacking hitherto, is obtained upon 'Reka' and 'Reglindis' as for their increased susceptibility.

• Fungicide resistant Trichoderma strains causing compost infection in shiitake production

  67-70.

  Gy. Turóczi

  M. Kovácsné Gyenes

  L. Vajna

  S. Balázs

  Views:

  118

  Trichoderma infection represents the major problem of shiitake production in the growing house of the Research Institute at Kecskemet. Heavy infections occur mainly on the compost before spawn run. All the isolated strain belongs to the species Trichoderma harzianurn, but morphology of the colony indicates that there are more strains liable for the infection. Source of infection remained unclear but the Trichoderma strains can be considered as weed moulds rather than true pathogens of shiitake. All the Trichoderma isolates showed unusual high degree of resistance to fungicides.

• Healthcare values and potential uses of the new Hungarian apple varieties on the basis on fruit analysis

  25-28.

  G. Ficzek

  M. Ladányi

  Zs. Radeczky

  M. Tóth

  Views:

  179

  Biological active compounds and valuable characteristics of some apple varieties and candidates were measured in our trials. Fruits of ’Rosmerta’, ’Hesztia’, ’Cordelia’ and ’Artemisz’ are recommended to enrich the Hungarian assortments for fresh consumption and choice of new tastes. Based on examined parameters it can be assumed that novel Hungarian resistant varieties are suitable for juice and fruit concentrate production, and due to high pectin content of their remaining pomace these varieties can be raw material of pectin production as well as they are also suitable for jam production mixed with other fruit species. Furthermore, functional food industrial product having high quality can be produced by using novel resistant varieties because of their high pectin and polyphenol content. Beside of their high market value their suitability for growing among orchard conditions is confi rmed by lower costs of production because of less plant protection treatments.

• Long term investigations of flowers and leaves on mainly non-domestica plums

  73-79.

  D. Surányi

  Views:

  161

  The author dealt with plum species representing different eco-geographic areas by their genetic adaptation and their hybrids, as European (P. domestica, P. italica, P. cerasifera), Asian (P. salicina, P. simonii, P. ussuriensis), American (P. americana, P. besseyi, P. munsoniana, P. tomentosa). The rootstocks of the trees examined were seedlings of C. 679 myrobalan with the exception of Laroda and Santa Rosa II, which were grown on three different stocks: seedlings of C. 174 myrobalan, C. 449 bitter almond and C. 471 sweet almond. The size of peduncle, length of pistil, stamen number per flower, relative stamen number (SN/PL) have been suitable for description and distinction of varieties. Similarly shape of leaves, length of petiole, length and width of blade helped the identification.

  The ratio of the dimensions of leaves, length of petiole and of leaf blade, also contributed to the distinction of European, Asian and American plum species, notwithstanding their relations with ecological conditions as well as historical, technical properties, pomological features, etc. Computed indicators (relative stamen number and shape-index of leaves) also have been useful data.

  Significant correlations have been found between colour of nectaries and mean values of variety-groups. The potential values of non-European varieties for purposes of commercial production could be forecasted from the point of view of quality, ecological, pomological as well as market value. It is important, however, to know the effect of the rootstock and growing site as well as their interaction, on the one hand, whereas the resistance or tolerance of the varieties as limiting factors, at least to the sharka (Plum pox) virus, Xanthomonas pruni, on the other hand (cf. Surányi & Erdős, 2004a and 2004b).

• Bean tissue culture and genetic transformation with Agrobacterium

  32-35.

  E. Eissa Ahmed

  Gy. Bisztray

  I. Velich

  Views:

  117

  In this paper we report the establishment methods of a rapidly growing callus culture of Phaseolus vulgaris bean as well as the conditions required for a high level of transient gene expression using Agrobacterium-mediated transformation. A vector is containing both the lindan-resistance gene as a selectable marker, and GUS gene as a screenable marker. By using hypocotyl explant and vertical culture on B5 medium supplemented with 1 mg/1 kinetin- and 2,4-D 2 mg/1 and subcultured every 3-4 weeks, we can recommend to get a good and much callus from bean. This will help in introducing foreign DNA into callus cells. One strain of Agrobacterium carrying plasmid as vector for introducing foreign DNA into plant cells was used. At different concentrations of lindan; 3, 4 and 4.5 mg/I, the transformed Maxidor callus survived and grew over a period of 6 month and subcultured every 3-4 weeks, but the control callus died. Callus were assayed for GUS activity to confirm the expression of the GUS gene using the histochemical assay test. The GUS gene was also correctly expressed in callus cultures grown on 4mg/I lindan-selected medium, the typical blue colour in the histochemical assay using the X-gluc as substrate. But the control, non-transformed callus was not able to grow in the presence of lindan, neither showed a positive reaction in the in vitro assays.

• Role of organosilicone surfactant in apple scab control under scab conducive weather conditions

  23-25.

  I. J. Holb

  Views:

  174

  Fungicides of integrated fruit production (dithianon, captan, and diclofluanid) and an organosilicone surfactant were compared in spray schedules from green tip until summer in order to control apple scab caused by Venturia inaequalis and to evaluate their phytotoxicity on fruit. Sixteen sprays of 1.8 kg ha^-1captan, 0.41 ha^-1 dithianon, and 1.8 kg ha^-1 diclolfluanid significantly (P=0.05) reduced the incidence of leaf or fruit scab compared to unsprayed products. All fungicides applied with organosilicone at 0.1% resulted in lower incidence of scab on young and older leaves as well as on harvested fruit, but these were not statistically always better than fungicides applied alone. In case of diclofluanid, the fungicide applied with organosilicone at 0.1% resulted in significantly lower (P = 0.001) incidence of scab on young and older leaves. Diclofluanid applied with organosilicone at 0.1% gave the best scab control on leaf and fruit. Treatments applied with fungicides alone had no significant effect on plant phytotoxicity compared to untreated control. All fungicides applied with organosilicone at 0.1% increased (P = 0.05) fruit damage compared to untreated control or fungicide applied alone, though these were not always significantly different. In case of percentage of fruit russet, treatment of dithianon 0.4 1/ha + 0.1% organosilicone significantly increased fruit russet, while fruit russet index significantly increased in the treatment of diclofluanid 1.8 kg/ha + 0.1% organosilicone compared to untreated control. In sum, application of surfactants can help to increase efficacy of scab fungicides; and consequently, to reduce the risk of fungus infection under high scab disease pressure. This fact may also be helpful in fungicide resistance management and reduced-spray programs with accurate scab warning systems.

• Controlling the southern root-knot nematode (Meloidogyne incognita Chitwood) with grafted and resistant pepper varieties

  33-37.

  Z. Mándoki

  Views:

  182

  Newly bred resistant bell pepper varieties and those grafted onto resistant rootstock s were tested in soil severely infested with southern root-knot nematode [Meloidogy11e incognita (Kofoid and White) Chitwood] in unheated plastic house and compared to varieties on their own roots, in order to evaluate the efficiency of this environmentally friendly control method. 'Cinema F I ' carrying the N gene yielded significantly more than the two susceptible varieties. Varieties grafted onto resistant rootstocks outyielded those on their own roots although to different extent, which was not always significant. At the end of the vegetat ion period the roots of the rootstocks were undamaged and the roots of some resistant varieties were slightly infected. whereas the roots of susceptible varieties were severely damaged. According to our result  . both the use of resistant varieties and grafted plants offer an effective and environmentally safe way of controlling M. incognita.

• Apple powdery mildew caused by Podosphaera leucotricha: some aspects of biology

  19-23.

  I. Holb

  Views:

  349

  Apple powdery mildew (Podoshphaera leucorticha) occurs wherever apples are grown. One of the most important fungal disease of apple which causing severe econimic loss on susceptible apple cultivars. Biology of the pathogen is widely investigated all over the world in the past 100 years. In this review, a summary from this enormous research is made for biology of apple powdery mildew in the following aspects: geographical distribution, morphology, taxonomy of the causal agent, symptoms, host susceptibility, resistance durability and disease cycle.

• Brown rot blossom blight of pome and stone fruits: symptom, disease cycle, host resistance, and biological control

  15-21.

  I. J. Holb

  Views:

  824

  In this paper, important features of symptoms, biology and biological disease management are summarised for brown rot blossom blight fungi of pome and stone fruit crops (Monilinia laxa, Monilinia fructicola and Monilinia mali). Firstly, European brown rot caused by Monilinia laxa is discussed highlighting the blossom epidemiology features, then host susceptibility of the most important stone fruit species including several Hungarian and international cultivars. At the end of this chapter, recent biological control possibilities against Monilinia laxa are also included. Secondly, American brown rot caused by Monilinia fructicola is discussed. Symptoms, biological features of blossom blight and host susceptibility of flowers to Monilinia fructicola are demonstrated. Finally, the symptoms and the biology of the least frequent species, Monilinia mali are shown.

• Experimental results of the effects of Hungarian climatic conditions to German disease-resistant industrial apple varieties

  53-56.

  B. Kápolna

  G. Kollár

  E. Henze

  Views:

  117

  In the recent years, several disease-resistant apple varieties appeared through the modern breeding technologies. These varieties can be grown with low usage of pesticides, which mean not only environment friendly fruit growing, but the production costs are also lower. In Eastern-Hungary — it is one of the main apple growing regions — a new apple growing structure started to form by the investment of the German Wink Ltd. — several resistant apple varieties were brought from Germany. 'Resistant' refers genetic resistance that usually transferred from the genome of wild apple species. But the fruit of these apple species is not only resistant to diseases, but its quality is poorer, too. In Germany the Re-apples are grown only for the processing industry. Due to climatic circumstances in Eastern Hungary, the first experiences showed better parameters during laboratory measurement, the fruits have more beautiful view, shape and inner characters than usual industrial apples.

  In our paper we discuss the results of sensory (consumer) tests, carried out in Eastern Hungary and in the Budapest-region the data analysis of systematic storing experiments (refraction, flesh firmness, weight loss, etc.) and profile analysis of fresh and stored Re-apples. (In the profile analysis the ProfiSens software [4,5] has been used.)

• High-velocity microprojectile mediated DNA delivery into Phaseolus vulgaris callus cells

  99-102.

  E. Eissa Ahmed

  Gy. Bisztray

  I. Velich

  Views:

  114

  We report the method for the establishment of rapidly growing callus cultures of Phaseolus vulgaris and the conditions required for efficient transformation using high velocity microprojectiles and high level of transient gene expression. Using hypocotyl explant and vertical culture on B5 medium with lmg/1 kinetin and 2 mg/1 2,4-D, we can recommend to get a rapidly growing callus from bean which is a good starting material to introduce foreign DNA into bean cells. The GeneBooster particle delivery system was used for the bombardment of bean callus and the Hgm resistance gene (Hgmr) was used as a selectable marker gene. 25mg/I hygromycin (Hgm) concentration was sufficient to kill the control callus. We used the standard physical factors, the appropriate pressure of N2 gas for the bombardment of the callus tissue, the shooting distance and the size of tungsten particles used as microprojectiles. Selective and nonselective tests were made by transferring the healthy green and white calluses, subcultured for 4 months on selective and nonselective medium. Several Hgm resistant calli had been obtained. Selective pressure was maintained over a period of 10 months.

• Ecological drought resistance and adaptability of apple varieties

  113-122.

  E. Nemeskéri

  É. Sárdi

  T. Szabó

  J. Nyéki

  Views:

  301

  For adequate yields in apple plantations, during the long growing period of the fruit primordia, one of the decisive factors is water supply. Indicators of stress are valuable signs for the diagnosis of drought and necessity of watering, i.e. planning the irrigation of plantations. The aim of the present study was to find reliable signs of water stress on apple trees and at the same time conclude on the drought tolerance of different varieties. The plantation of apple varieties grafted on various stocks and cultivated according to different systems (irrigated, non irrigated, integrate and biological) has been examined continuously by leaf analysis. Along the period of growing fruits, measurements were made in the field, then the leaf samples were analysed in the laboratory for composition of pigments, carbohydrates and antioxidants, as well as the histology of the tissues checked. Without irrigation, the mean leaf mass and the relative chlorophyll content (SPAD) of the variety Idared on M4 stocks increases beyond the values of 50 SPAD, whereas on M26 stock and integrated system, it declines. In the collection of varieties, grown according to the „integrated” system, during the development of fruits, ‘Gála’ and ‘Remo’ varieties have been affiliated to the “less susceptible” group regarding drought tolerance because of the leaf morphology, structure and content of SPAD and antioxidants. However, ‘Idared’ and ‘Jonagold’ belong to the “susceptible” group. To the same group are ranged the ‘Akane, Red Rome vanWell, Pink Lady’ varieties. Those varieties reacted to a short period of drought by increased production (content) of carbohydrates. Regarding changes of carbohydrate content ‘Greensleeves’ and ‘Ozark Gold’ varieties belong to the moderately water dependent (requiring) group. In the field, SPAD-tests facilitated the measurement of nutrient-uptake and incorporation, which is proved by the tight correlation between the data of SPAD and the increment of leaf weight (r=0.76–r=0.88), however, this depends on the variety too. SPAD is an indicator of water supply and is related with the density of stomata, cannot used for the selection of water-exigent varieties but for (drought) tolerant ones. In integrated culture, the (drought) susceptible varieties display (water-soluble) hydrophilic and lipophilic antioxidants (ACWand ACL resp.) the quantity of which may have some role in drought tolerance.

• Blumeriella jaapii (Rehm) v. (Arx) infection of some sweet cherry cultivars in two years with different precipitation conditions

  37-39.

  K. Király

  T. Szentpéteri

  Views:

  193

  We examined Blumeriella jaapii (Rehm) v. (Arx) infection on 21 sweet cherry cultivars in the cultivar-collection of the Experimental Station Pallag of the University of Debrecen, in 2004 and 2005. Considerable differences were detected in the infection degree of the different cultivars. In 2005, due to the more humid weather, the average infection highly exceeded data of the previous year. No symptomless cultivars were detected in either year. The least sensitive ones were Linda and the cultivar 11/106. The most sensitive cultivars were Early Müncheberg, Biggareau burlat and Round Solymári. Infection degrees of Alex, Rita and Vega were different from those of other cultivars on the basis of the two experimental years.

  It is important to consider both the specific weather conditions of the year and cultivar resistance at planning the plant protection system. It is recommended for each cultivar to perform a fungicide-spaying after the harvest.

• Calla lily soft rot causal agents, symptoms, virulence and management: a review

  60-68.

  A. N. Niyokuri

  S. Nyalala

  Views:

  111

  Bacterial soft rot is a polyetiological disease attacking calla lily [Zantedeschia spp (L.) Spreng.]. This disease has reduced the commercial value of this crop. This work aims to review scientific information to give an insight into calla lily soft rot causal agents, symptoms, factors favouring the disease, virulence mechanisms and management strategies. Special emphasis is put on the current progress with regards to understanding calla lily mechanisms of resistance to soft rot and their potential for the development of tolerant/resistant cultivars with commercial traits.

• Relation between the germination and infection ratio on Sida hermaphrodita L. Rusby seeds under hot water treatment

  117-121.

  E. Kurucz

  P. Szarvas

  M. G. Fári

  Views:

  173

  Sida hermephrodita or virginia mallow is a perspective perennial herb in the Malvaceae family able to yield a biomass crop through between ten and twenty years. Additionally, the plants have a lot of uses and benefits for instance it can use it as a fodder crop, honey crop, ornamental plant in public gardens. It has favorable features like fast growing and resistance against the disease and climatic fluctuations, etc. Sida is in base stage of domestication therefore has a serious disadvantage the low and slow germination as a big part of wild plants. Due to the expressly low germination percent the need of seed showing of driller is should tenfold, 200 thousand seed/acre instead of 10-20 thousand what is not available and expensive Therefore practical purposes of our research of seed physiology was to increase the seed germination percent in a disposable ,basically wild Sida population. We examined two factors relating to seed germination percent and seed germination power during our research: the influence of hot water treatment and the effect of exogenus or endogenus infection of seed. However, in our germination tests, utilizing scarified seeds with hot water (65, 80 and 95 oC), 29,33 to 46% germinated of the seeds collected from the population of S. hermaphrodita in Debrecen. The average germination for all season was 5-10 % wihitout treatment and rised using hot water up to almost 50%. When physically scarified used, the oldest seeds showed the best germination (46 %) after the hot water operation in spite of the previus studys (Spooner 1985; Chudik et al. 2010; Doliński R. 2009.). We discovered that there are a distinguished close relationship between the seeds collecting time and the infection, as well as germination percentage. Thus, 2009 season was the most favourable in case of contamination (control:17,33 and 80 oC treatment:0%) as well as germination percent. It could be concluded that, the best season for our findings was 2009 due to autumn harvest of Sida seeds. In our oppinion, the autumn harvesting should be the best time to overcome the problem of the low germination and high infection percentage.

• The development and reproduction of grape phylloxera (Daktulosphaira vitifoliae Fitch) strains of Hungarian vine-districts in root-bioassays

  67-71.

  H. L. Tóth

  L. Horváthné Baracsi

  L. Kocsis

  Views:

  132

  The root bioassay method allows for 4-6 week continuous observation of grape phylloxera feeding on the grape root. 10 Hungarian phylloxera strains were compared on susceptible Vitis vinifera cv. Chardonnay and the resistant rootstocks of V. berlandieri x V. riparia Teleki 5C and V. berlandieri x V. rupestris Georgikon 121 in in vitro observations. The strains originated from Villany and Eger (Figure 3) had higher reproduction on the root of V. berlandieri x V. riparia Teleki 5C (201 and 119 eggs) and were more aggressive than the others (average production 10 eggs). The continuous high level of survival, development and reproduction of the Eger strain on Teleki 5C (V. berlandieri x V. ripuria) until day 46 may be due to adaptation.

• The Effects of Some Parameters on Agrobacterium-Mediated Transformation in Muskmelon

  46-49.

  Z. Qiu

  Cs. Bársony

  Gy. Bisztray

  I. Velich

  Views:

  170

  Some parameters involved in Agrobacterium-mediated transformation in muskmelon Hales best (HBS) were studied. Cotyledon explants excised from 3.5-day-old seedlings were co-cultivated with Agrobacterium tumefaciens harbouring binary vectors which contained GUS and BAR genes. After co-cultivation on a low pH medium, explants were transferred to selective medium, with higher pH, containing Claforan and Finale. The medium was changed every two weeks till shoots were induced. All shoots rooted on MS medium supplemented with 0.3 mg/L IBA. These parameters combined as a whole led to successful transformation. The expression of the introduced gene construct was confirmed by GUS staining of shoot segments.

   

• Genetic transformation of bean callus via Agrobacterium- mediated DNA transfer

  49-53.

  E. Eissa Ahmed

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  120

  Callus cultures were induced from hypocotyl of young bean seedlings. Callus developed and maintenaned on B5 medium supplemented with 2mg/1 2,4-D and 1 mg/1 kinetin. The results demonstrate that A. tumefacins-mediated transformation is a convenient method to obtain transient gene expression in callus of bean. The results have shown that the bean callus co-cultivated with A. tumefaciens can be transformed to get heibicide Finale (glufosinate-ammonium) resistant GUS positive tissues. Southern blot analysis of transformed calli showed integration of gusA marker gene carried by a binary vector. Transformed calli were selected on herbicide containing media. Data of molecular analysis (Southern blotting) confirmed the insertion of gusA gene in the genome of herbicide resistant calli with bar gene. There are three evidences that calli are stable transformants: (1) herbicide resistance, (2) GUS activity which is indicative since the coding region containing an intron, (3) the results of Southern hybridization technique.