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Evaluation of elder (Sambucus nigra) varieties and candidates for the canning industry. Results of the composition studies
102-107.Views:277Sambucus nigra is a very common elder species in Europe. Due to its excellent composition, natural dye content and healing power it can be considered as a biological active plant. In Hungary the cultivation of Sambucus nigra started in the nineties and since that time there is a growing demand for this plant. Sambucus nigra is a special fruit due to its sole.-application for processing industry. Partly fruit products are made of it, partly it is used as a natural dye. In both cases the first step is the knowledge and evaluation of the composition of the various elder varieties and candidates. For that reason composition studies of different elder types were performed in several years at the Department of the Canning Technology of the Szent Istvan University.
The most important sample was the Haschberg variety, which is the sole elder type accepted and allowed for propagation by the government. This plant is grown in the Pilot Plant of the Szent István University in Szigetcsép. Among the studied samples there were some varieties cultivated in the Fertőd Research Station. They were the following types: No. 33, 480 and 481. Besides elder collected wild in Szigetcsep and the very promising early-ripened SZ-CS 21-23 variety originated also from Szigetcsép were investigated. One year it was possible to study the Sambu variety, too. Department of the Fruit Culture of the Szent István University helped to obtain the various elder types and candidates. Along our experimental activities, the following components and parameters were studied: the content of pectin, organic acid, carbohydrate, mineral, vitamin C, total acid, aquesoluble dry material, anthocyanin and the pH.
After having obtained a juice, a concentrate from the species has been made and — after freezing — the aquesoluble dry substance and the acid content, as well as the colour parameters were monitored. Studies were performed in two years (1997-98). On the basis of these results classes were established for all compounds, from which an overall ranking was derived.
It was found that in both years the Haschberg variety proved to have the best composition. Therefore regarding to the overall series of order the experiments performed in the second year supported the results obtained in the previous year. Results have shown that Sambucus nigra has excellent composition beside its curative effect. Its mineral content has surpassed that of the other fruits and it had advantageous acid-and carbohydrate composition. The ascorbic acid content of the Haschberg variety is striking. It can be concluded from the experiments that the Haschberg type has the most advantageous composition, therefore it is suitable for making special curative and exclusive products.
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Grape variety comparison of different stress tolerance based on the quantitative measurement of carbohydrates
37-40.Views:350The analyses of various host-pathogen relationships have established the response reaction roles of carbohydrates — especially monosaccharides — measurable in the vegetal parts of the host. Published results also provide information concerning the way various pathogens utilize carbohydrates and concerning the carbohydrates pathogens prefer out of the "selection" provided by the host plant. The role of carbohydrates in the response reactions to abiotic stress has been studied on several plant species as well — currently, too, it is an often discussed area of research. The above-mentioned results form the basis of our intention to study the connection between susceptibility to grey mould and the quantity of measurable carbohydrates in the leaves of grape varieties of various stress tolerance levels.
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Evaluation of fruit tree transpiration with heat flux sensor
11-13.Views:369One of the biggest professional challenges of the following years is to develop the water resource management for apple trees. In order to know the water consumption of plants, some traditional and modern methods could useful. In our research the sap flow measurement of the plants were carried out. We have analyzed the transpiration properties of the trees in the investigation period. In order to compare the transpiration of the trees, the leaf areas were scanned and evaluated. Beside these measurements, soil water properties were determined by tensiometers, thus it makes possible to observe the soil-plant-atmosphere system.
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Training and maintaining spindle crowns in cherry production
51-53.Views:633In cherry production all over the world, intensification of the technology is the main objective of research. Small crowns and high planting densities are aimed to attain high yields per hectare and easier harvesting. Rootstocks of reduced vigour for cherries are more difficult to find than in other fruit species, and the rejuvenation of fruiting structures by pruning is aggravated by the reduced vigour. Intensity of the technology ought to be achieved by a thoughtful application of the technological elements (timing of pruning by various intensity) moreover, by finding different policies for individual varieties. Sweet cherry varieties dominating the assortment proved to be very variable regarding their growing habits. In our experiment, we dealt with the slender spindle and free spindle forms, and how to train the trees to develop and to maintain the desirable form depending on the respective variety in order to achieve the right load of flower buds and yields repeatedly. In this paper, we examine the most important practical issues with the training and maintaining of the crowns of cherries with circular projection and central axis grafted on Prunus mahaleb rootstocks
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Water relations of sour cherries (minireview)
103-107.Views:371Recently, the sour cherries as food resources become more important for health preservation and so the modernization of growing technology in sour cherry production will be timely. The global warming and inadequate distribution of precipitation result a decrease in the alternancy of sour cherry production, as well. Sour cherries rather adapted to survival of drought than sweet cherry trees therefore a few studies performed to explore the water requirement of sour cherry varieties. The rootstocks, the type of soils in plantation and the water balance influence the water management of sour cherries. In orchards, in particular first year plantation, use of various row covering contribute to preservation of the natural water pool of soil and affect on the tree vigor, yield and fruit quality. Wide-spread application of integrated fruit growing technology and climate changes the researches are pointed to develop efficient irrigation technology based on transpiration yield model. The crop model based on use of meteorological data was developed for cherry orchards in order to predict transpiration of trees, dry matter production and fruit yield. The linear relationship between dry matter accumulation and transpiration was verified for sour cherry trees. Other models essay to asses the effects of climate changes on crop production. Importance of economical production and fruit quality such as ingredients of raw materials and food increases in intensive sour cherry orchards used by irrigation techniques. Because of climate changes it should more pay attention to research concerning on the stress physiological response of sour cherry varieties and post-harvest fruit quality.
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Genetic variation in the rose pathogen Marssonina rosae estimated by RAPD
63-67.Views:313Blackspot, caused by the fungus Marssonina rosae (Diplocarpon msae), is one of the most devastating and widespread diseases in garden roses, and it has as yet not been fully characterized in molecular terms. In this initial study we used RAPD analysis to investigate the genetic diversity among and within a few geographically diverse groups of single-spore isolates of M. rosae. DNA was extracted from in vitrogrown mycelia of 1 I single spore isolates grown on PDA medium. High levels of polymorphism were detected among the isolates. They clustered into three distinct groups: Group !consisted of isolates from eastern North America plus a European isolate (Germany), Group 2 included isolates from southern Sweden, and Group 3 included the isolates from Manitoba, Canada. The greater similarity of the environmental conditions in eastern North America and Europe as compared to the Canadian prairies suggest that. climate and weather could be key factors in influencing the potential race structure of M. rosae. However, variations among closely situated sites, e.g. southern Sweden, also occurred.
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Character association and genetic gain of nine agronomic traits of F1 populations in onion (Allium cepa L.)
50-58.Views:694F1 populations of half diallel cross were considered for this investigation. Phenotypic component of variation (s2P) was higher than genotypic (s2G), interaction ((s2I) and within error (s2w) components of variation. Trait bulb weight showed the highest values for s2P, s2G, s2I and s2w. The noticeable amount of phenotypic, genotypic, interaction and within error covariation was found in the combination of BW×BY, possibly indicating wide scope of selection for this pair of characters. Genotypic correlations were higher than the respective phenotypic correlations. This situation was also marked in the path coefficient analysis. Bulb yield/plot showed highly significant and positive correlation coefficient with other characters both at phenotypic and genotypic levels. When all the nine characters were included in an index, it exhibited the highest genetic gain as percentage. When a combination of two or more characters was studied in a function, the efficacy was higher than that of direct selection for bulb yield. The combination of five, six, seven or eight characters showed higher percentage of expected gain. Due to significantly correlated with BY and having high positive direct effect at phenotypic level characters viz., LL, BW, PH and NLs is considered as primary yield components. Again combinations of these four characters gave the commendable expected genetic gain of 330.7290% may be considered as important selection index for this material.
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Relative ecological and biological indicator values of plum and prune cultivars
37-53.Views:441The study was conducted to compare historical plums, gene collections, and is currently cultivated and recent perspective varieties from the author. The object of the study was the relative ecological Borhidi’s figures and classification of varieties under the new definition, as the relative biological values. There were 11 figures no one in particular affect the data of the plums grown in importance as TB, WB, RB, LB, and the values are relative biological value (OP, FR, SS, and DR). The specificity of each indicator was different, but in general the importance and specific breeds was associated with. The SB (salt figures) is not proved informative, partly because small data in the literature, on the other hand, had little to their own observations as well. The gene bank of Cegléd is now third-generation (1951–1972, 1973–1991, since 1992) kind of collection, during which many aspects have changed climatic conditions: cold winter eased the strictness, but has increased the threat
of spring frost advection. The relative biological scores markedly influenced around flowering extreme weather (dry, windy weather and so flowering in within 2-3 days), optimal conditions of the plum pox virus vectors and of course the presence of fruit and foliage harmful fungi. It see to be, in the case of semi-wild and wild fruit species – are possible with similar comparative analyzes, and hopefully will in feral forms, culture – as we have seen previously (Surányi 2000 and 2006). In the continuation analysis of the natural vegetation and cultural context of the complex multifactorial factors will be carried out more easily, according to the relative value figures, as well as rootstock effects and plantation’s habitat studies, and even the most phytotechnical evaluation of interventions. -
Actual state of research concerning vitamin C as reflected in the literature (Review article)
7-15.Views:641Vitamin C (L-ascorbic acid) being essential for many living organisms, including man, became once more into the focus of interests because of its numerous physiological effects. Its anti-scurvy and anti-oxidant properties have already been recognised since long in the human body, but it turned out gradually that it has many other functions. In plants, its primary importance is defense against the photo-oxidative stress.
The present review is intended to reveal some details of the artificial synthesis of vitamin C. Emphasis is put on the metabolism of L-ascorbic acid in higher plants. Biosynthetic processes, translocation and accumulation are discussed in detail on the basis of recent results published in the scientific literature.
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Inheritance of blooming time in walnut, with regard to the property of reproductional autoregulation of species
118-122.Views:226A great number of crosses have been made with Hungarian and foreign varieties as partners to breed improved varieties. This species shows a particular trait, namely the autoregulation of fruit set, which affects considerably the productivity of commercial orchards. Thus the inheritance of the blooming time of the male and female flowers has been explored for several years in the progenies.
It has been stated that
- the feature of the partners does not turn up predictably in the progeny,
- it is most important to take into consideration the blooming time class of both, male and female flowers in planning associations of varieties for commercial orchards.
- in years of irregular spring weather the stability of the blooming time of the variety or in other words the deviation of the actual blooming time of variety from its characteristic blooming-time class is also very important.
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Evaluation of introduced plum varieties under extreme climatic conditions
7-10.Views:504In the gene bank variety collection, 15 plum varieties were examined based on morphogenetic and pomological traits. Most cultivars of self-fertilizing or pollinating partners were well-cultivated. In addition to Queston, Stanley, Valor and Verity, towards 11 new cultivars have been experienced in this paper.
From the observations, it seems that the growth and plantation of Valor, Presenta, Topfive and Bellamira can increase. However, the Top Sugar and Valor fruit sizes are best suited to market requirements. The role of Stanley may be reduced, but there is no reason to replace it. However, the questions of local varieties of domestic origin fall into a different category, and raw materials for quality products are difficult to replace with other varieties.
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Preliminary characterization of the self-incompatibility genotypes of European plum (Prunus domestica L.) cultivars
23-26.Views:521European plum is an important fruit crop with complex, hexaploid genome of unknown origin. The characterization of the selfincompatibility (S) locus of 16 European plum cultivars was carried out using the PaConsI-F primer in combination with the EM-PC1consRD primer for the first intron and the EM-PC2consFD and EM-PC3consRD primers for the second intron amplification. Altogether, 18 different alleles were scored indicating high genetic diversity. These alleles were labelled using alphabetical codes from SA to SS. We identified 5 different alleles in 9 cultivars, 4 alleles in 5 cultivars, while 3 alleles were shown in two of the assayed cultivars. A total of 16 different S-genotypes were assigned, and discrimination of all plum cultivars was successful based on their unique S-genotypes. However, further research is required to reliably identify the S-alleles based on their DNA sequence and clarify complete S-genotypes.
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Rootstock effects on fruit drop and quality of 'Arlet' apples
69-75.Views:947The aim of this study is to investigate the effect of different growth inducing rootstocks on fruit drop of apple. This research was conducted for 3 years at Nagykutas, Western Hungary on apple cv. 'Arlet'. For the experiments, 3 different growth inducing rootstocks were choosen: M.9 (weak), MM.106 (moderate) and crabapple seedling (strong). There were 3 fruit shedding periods on the trees grafted M.9 and MM.106 rootstocks, but in the case of crabapple seedling, only 2 were found. The measure of fruit drop was closely related to seed count of fruits; seed number was the lowest, fruit drop was the highest. The lowest seed number was counted in fruits from trees on crabapple seedling. Seasonal changes of leaf:fruit ratio mainly depended on shoot growth and fruit drop. The rise of the curve of leaf:fruit ratio was very important during the first phase of fruit development, in especially at the end of June and in the beginning of July. The cause of this is that first and second periods of fruit drop appeared during this term. The rise of the curve was important in the beginning of June and the end of July on crabapple seedling. Decreasing tendency of quality parameters was found of fruits from trees on M.9, MM.106 and seedling rootstocks, except flesh firmness which was the highest in fruits from trees on crabapple seedling.
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Comparison of weed management methods in organic carrot
55-58.Views:23414 combinations of mechanical and also physical (thermal) weed management techniques are compared for organic growing of carrot. Crop of our weed management research is carrot because of its difficulties in weed management (long growing period, poor weed tolerance) and because carrot needs to be important product of organic farming. Herbicide treatment is used as control — cultivator, brush hoe, hand hoeing and hand weeding are mechanical control tools and flame weeder is used for thermal control. Measured parameters are the weed cover, cover of the crop and dry mass of them. Result of the two appraisable years shows contradictory results, which contradiction can be justifiable with different weather conditions of these two vegetation periods. In 2000 brush hoe was significantly the best in interrows but in the year 2001 cultivator combined with hand weeding in rows seemed to show the best result. We can see in this example that agriculture and weed management depends very much on the weather of the year, so that is why it is so difficult to develop a method, which can be generally used for organic weed control of carrot.
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Reactions of some cucumber (Cucumis sativus L.) lines and hybrids to zucchini yellow mosaic virus (ZYMV) and selection of tolerant breeding lines
66-68.Views:305In the past years zucchini yellow mosaic virus (ZYMV) has been appeared as a new pathogen of cucurbitaceous plants in Hungary. It caused severe disease outbreaks on the fields of pickling cucumber hybrids which are highly tolerant to cucumber mosaic virus (CMV). Preliminary inoculation tests have showed that all of the important pickling cucumber hybrids produced in Hungary were susceptible to ZYMV-P. However, a selection of Chinese Long and the breeding line I- KS 10C proved to be highly tolerant. The Fl plants of the crosses between the tolerant lines and the susceptible genotype H I were susceptible to ZYMV-P. F2 populations segregated for susceptible and tolerant individuals at a ratio of 3:1. The results strongly suggest that the resistance to ZYMV in our sources is controlled by a single recessive gene. Inoculations of the ZYMV and CMV susceptible cultivar Budai csemege and the CMV tolerant hybrid Perez Fl with the complex of ZYMV+CMV resulted extremely severe symptoms (strong mosaic and necrotic spotting of the leaves) on both cultivars. On the Chinese Long line, which is tolerant to ZYMV and CMV, respectively, the complex of the two viruses caused mild symptoms. The results show a synergistic pathological effect of ZYMV and CMV on differrent cucumber genotypes. More detailed studies on the interactions among the plant genotypes, viruses and virus strains are needed to develop cucumber hybrids that are highly resistant to the ZYMV+CMV complex.
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Fruit drying traditions in Hungary
7-10.Views:623Since the early eras of human history, the gathered products (roots, shoots, crops, fruits etc.), the environmental conditions of the preservation of the animals caught and the fishes; and accidental observations have been born new methods. Under cold and moderate climates, the effect of permanent frost, the use of ice as a means of curing as in dry, desert areas is the drying or exsiccation and the dehydration of salt. For convenience reasons, some of these methods have been forgotten, but the resurfacing of different fruits seems to resume. The short review gives an overview of the methods of drying used in the Carpathian Basin, especially among the Hungarian ethnic groups, which by the Hungarian ethnology thoroughly explored. The author attaches outstanding importance to the fruit-drying industry, because dried-ups at industrial scale are not equivalent to traditional low-fat lime scales. Excellent drying products are much better in terms of eating habits, with modern methods and small-scale production relatively easy to produce.
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Preliminary results of renewal pruning an 18 years old sour cherry trees
41-42.Views:310Sour cherry is a light demanding fruit species. As most of the crop is developing from buds on one year old shoots and 2-yearold wood, the formation of bare wood can be seen apparently in a badly maintained canopy. The formation of „whip shoots” indicates the degree of bare wood. Unfortunately in many gardens and commercial orchards sour cherry trees resemble willow trees. Owners often decide to grub old orchards, although the useful cropping lifespan of the trees planted in a correct spacing can be 20-30 years on a move vigorous rootstock. Therefore renewal pruning can contribute to the full renewal of the orchard, but the length of its effect is depending on the growth characteristics and renewal capabilities of the cultivar. The growth characteristics of the cultivars are different. The regeneration capabilities of different aged wood are also different. These differences emphasize the need for cultivar specifi c pruning. Knowing the reactions to renewal pruning, we can ensure regular high crops with excellent quality by applying cultivar specifi c rotation pruning.
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Effect of boron nutrition on nutrient uptake and fruit quality of tart cherry in Eastern Hungary
45-48.Views:739Effect of the foliar boron (B) application on nutrient uptake and fruit quality of tart cherry (Prunus cerasus L.) was investigated from 2008 to 2009 on mature four favourite Hungarian tart cherry cultivars like ‘Oblacsinszka’, ‘Érdi bôtermő’, ‘Újfehértói fürtös’ and ‘Petri’. Tart cherry trees grown in Eastern Hungary (Újfehértó) on an acidic sandy soil with low B content. Trees were treated with B by foliar application (0.5% B) at full bloom and untreated with B served as a control. B sprays strongly affected on B content of inflorescences. However, B sprays had no consistent effect on summer leaf B status. In our experiment stronger year effect was observed at leaf B status than flower B status. The effectiveness of early spring boron applications are limited and mostly affected the flower B status only. Moreover, treatments had inconsistent effect on studied fruit inner parameters. Monosaccharides, vitamin C and organic acid contents of sour cherry were stronger affected by cultivars than applied treatments.
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Molecular identification of old Hungarian apple varieties
37-42.Views:751Altogether 40, mainly old Hungarian apple varieties were screened with six previously described microsatellite markers. A total of 71 polymorphic alleles were detected (average 11.8 alleles/locus) and the heterozygosity of markers averaged very high (0.8). The genetic variability among the genotypes proved to be so remarkable that as few as three markers from the applied six were enough to distinguish between the 40 varieties. This was also confirmed by the cumulative probability of obtaining identical allele patterns for two randomly chosen apple genotypes for all loci, which value was quite low: 2.53 x 10-5. The molecular identification of these genetically very different old apple genotypes could be very useful in future breeding programs.
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Molecular analysis of strawberry cultivars using RAPD, AP-PCR and STS markers
24-28.Views:285Seventeen strawberry (Fragaria x ananassa Duch.) cultivars representing the national list of Hungary, were subjected to RAPD, AP—PCR and STS analysis. Of the 31 decamer and oligomer primers tested 26 primers produced polymorphic patterns. 45 polymorphic fragments were analysed, ranging between 200-2800 by in size. Based on the data, similarity coefficients (Jaccard index and Simple matching coefficient) were calculated, and dendrograms were constructed using the unweighted pair group method of arithmetic averages (UPGMA). The dendrograms only partly reflect the known pedigree data. Specific RAPD markers were identified for cultivars F5, Pocahontas and Rabunda.
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Nectar production of quince (Cydonia oblonga Mill.) cultivars
87-94.Views:1253Nectar production of six quince cultivars were measured during three consecutive years. The nectar production of quince can be regarded to be small compared to other temperate zone fruit tree species since quince flowers contained 1.07 ± 0.06 mg of nectar in average. The extreme values, however, ranged between 0.1 and 7.3 mg/flower and this indicated that the nectar production was highly variable. The distribution of the nectar production was definitely skew because low values were definitely much more frequent than the highest ones. Our findings do not corroborate the earlier statements on the high sugar concentration of quince nectar. We found some 21-27% sugar in average, only. The normal distribution of the sugar concentration also indicates that the typical sugar concentration may be between 20-30%. Accordingly, the sugar concentration of quince nectar is rather low compared to other temperate zone fruit tree species (except pear). There was a significant negative correlation between the amount of nectar and its sugar concentration in quince flowers in all of the three years of the study (r= -0.51, n=37, p<0.02 in 1996, r= -0.57, n=28, p<0.1 in 1997, r= -0.35, n=9 I, p<0.001 in 1998). No definite difference was established between the nectar production of quince cultivars. Nevertheless, one cultivar tended to produce less and two other ones produced somewhat more nectar in average than the rest of the 6 cvs investigated but the extreme values of nectar production of cultivars overlapped in most cages.
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COVID-19 outbreak lockdown and its impacts on marketing of horticultural produces in Zimbabwe
38-45.Views:835Coronavirus disease of 2019 (COVID-2019) outbreak resulted to a global health problem. Lockdown was one of the common options suggested to curb the pandemic. Horticultural produces are perishable so delayed post-harvest marketing cause losses. This study aimed at evaluating the marketing of horticultural produces and farmers’ level of preparedness during the COVID-19 lockdown. A self-administered questionnaire was posted online from April 7 to May 7, 2020 the first month of COVID-19 lockdown in Zimbabwe (began on March 30, 2020). The study targeted horticultural farmers around Harare peri-urban and had 300 respondents. Data on sociodemographic, farmers’ behavior, awareness and attitude toward marketing and desire to minimize post-harvest losses was obtained. Frequency counts and percentages were determined. Chi-square tests on independent variables were done to test associations with demographics using SPSS version 8. There were significant (P<0.05) relations between awareness and attitude toward marketing, age and level of education. Demographic variable influenced the famers’ marketing behavior and attitude during COVID-19 lockdown. Most farmers reported highest (>35%) produce sale leftovers at the markets and spoilage during than prior the COVID-19 lockdown. The lockdown resulted to low sales and most farmers were unprepared for any crisis during the marketing stage of their production. The research contributed to an understanding of how a crisis situation influence marketing of horticultural produce and raises awareness regarding post-harvest losses. A qualitative study is recommended as a follow-up of this work.
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In vitro comparative study of two Arundo donax L. ecotypes’ selenium tolerance
119-122.Views:550Selenium tolerance of two somatic embryo-derived Arundo donax L. ecotypes (Blossom, 20SZ) were compared in in vitro culture. Sodium-selenate (1 – 100 mg L-1) as known the most phytoavailable selenium form and the less studied red elemental nanoselenium (100 mg L-1) were applied as selenium treatments. Basis on the results Blossom ecotype seemed to be more sensitive to the sodium-selenate than 20SZ. Inhibiting effect of selenate was effectuated above 10 mg L-1 in case of Blossom, which was manifested in decreased survival rate and growing parameters. Contrast to this 20SZ could tolerate the selenate ≤ 20 mg L-1 without any toxic symptoms. Lower selenate tolerance of Blossom could be explained with higher selenium accumulation. Both of two ecotypes could also uptake and accumulate the red elemental nanoselenium however in much less extent compared to selenate.
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Relations of phenometrical indices of apple fruits with weather variation in the assortment of varieties of an apple gene bank
115-120.Views:932The purpose of phenometry is to examine the measurable parameters of the plants in order to follow up the consequences of weather processes. We should fi nd the reasons, why the diameter of fruits grows larger in one season and smaller in the next. Variation may occur as a response to insuffi cient provision of water or nutrients, but also because of pathological effects and of extremely high or low temperatures, moreover, of extraordinary heavy fruit load. There are phenometrical characteristics, which consider the fi nal consequences (density of fl owers, fruits set, drop of fruits), whereas other parameters could be followed up (size, length and width of fruits) as the dynamic components of growth. The quantitative parameters of growth are functionally related to each other, where the weather conditions, soil humidity and nutrients are on the input side, thus it is possible to model the growth of fruits as a function of the environment. Initially, the relations between the main weather variables and the phenometrical data have to be cleared. In the present study, the interactions between the mentioned phenomena are presented and numerically defi ned.
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Stimulating effect of distilled water
47-49.Views:351It is an early observation that plants in poor soil are developing roots quicker and more abundantly than on rich one. There is a similar correlation between the nutrient status of medium and adventitious root formation.
In order to throw more light on the background of this strange phenomenon we started a systematic experimental program in which the biological effects of distilled water as model factor was investigated.
The experiments proved that the root formation of Pinto bean (Phaseolus vulgaris L.) cuttings with 3 cm long hypocotyls was promoted by distilled water.
The phenomenon above accompanied with slower decline and faster recovery of total and also water-soluble protein content, more intensive efflux of amino acids, greater amount of tryptophane and increased uptake of water compared to those in control hypocotyls. From other data obtained we may suspect that some additional active substance unknown for us also contributes to the stimulation of root initiation in distilled water.
