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Abnormalities of the stigma of sour cherry cultivar
31-33.Views:4800The objective of this study was to evaluate the ratio of blackness of the surface of stigma of sour cherry cultivars. At the full bloom time of sour cherry 100 new opened flowers were marked in the internal (Inside), external (outside), bottom and upper parts of the crown of each cultivars including sour cherry cultivars ‘Érdi bőtermő', `Debreceni bőtermő', `Kántorjánosi', 'R. clone', 'Petri', Pándy', and 'D. clone'. The trees were replicated four times. The numbers of flowers with black stigma were counted and the percentage of dead stigma was calculated. In addition, tissues of black stigmata were investigated for blossom pathogens by microscopy. After flowering time the fruit set of the marked flowers counted and then percentage fruit set was calculated. Numbers of counted flowers were between 300 and 980 depending on the four position of the tree. Black color of stigma could be seen only on three cultivars (`Debreceni bőtermő', Érdi bőtermő' and 'Petri') out of seven assessed cultivars. The highest numbers of black colored stigma were found on cultivar ‘Érdi bőtermő' which ranged between incidences of 12 and 21%. Black stigma was never able to produce a fruit set. Microscopic examination revealed no pathogens associated with black stigma. Different part of the tree resulted different amount of black stigma. Black stigma was the largest on the outer part of the tree on cv. 'Érdi bőtermő' but also bottom part of the tree also produced larger number of black stigma on cvs. `Debreceni bőtermő' and ‘Érdi bőtermő'. Though symptoms were not typical to frost damage, we believe that black stigma is probably due to environmental factors during flowering. This might be associated with late spring cold coming from the soil surface as the bottom and outer part of the tree was more suffered from the disease.
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Fruit Quality of Sweet Cherry Cultivars Grafted on Four Different Rootstocks
59-62.Views:411A rootstock trial planted at the Szigetcsep experimental station in 1989 involved the study of two cultivars- `Germersdorfi FL 45' and 'Van' -grafted on four different rootstocks — 'Colt', `MxM 14 — Brokforest', `MxM 97 — Brokgrow' and 'Saint Lucie 64' as a control. The trees were trained to the "Modified Brunner-spindle" system and came into bearing in 1993. The yield per tree, fruit weight and fruit diameter have been measured each year since then. The refractivity, the acid content of the fruit juice, fruit cracking after four hours' dipping in water and stone weight ratio have also been measured since 1995. In 1997 and 1998 these parameters as well as fruit cracking after 24 hours' dipping were measured. Fruit firmness and fruit colour were also estimated. In almost every observed parameter significant differences were found between the scion cultivars. Yield efficiency was significantly higher on 'NUM 14' and 'Saint Lucie 64' than on the other two rootstocks. As regards fruit weight, in both cultivars and over an average of six years, it was found that trees on low yielding tree on 'Colt' rootstock had the highest fruit weight values and on heavy producing `MxM 14' the smallest. Soluble solids content was higher on 'Colt' and `MxM 97'. No significant differences between the rootstocks were found in acid content of the fruit juice. There were significant differences between the rootstocks in fruit cracking after 4 and 24 hours' dipping in water. Seemingly with respect to cultivars and rootstocks the year has a considerable effect on fruit cracking.
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Epidemiology of brown rot on two apple cultivars in an organic apple orchard
49-52.Views:575In a two-year-study, the temporal development of brown rot (Monilinia fructigena) on fruits was analysed in an organic apple orchard on an early (Prima) and one late (Idared) maturing cultivars at Debrecen-Józsa in Hungary. Out of five mathematical functions (linear, exponential, three-parameter logistic, Gompertz, Bertalanffy-Mitscherlich), the three-parameter logistic function gave the best fit to brown rot incidence of all cultivars in both years. Disease progress started at the end of June for cv. Prima and at the end of July for cv. Idared, then disease increased continuously from 6-8 weeks up to harvest in all cultivars. Descriptive disease variates derived from the three-parameter logistic function were used to analyse disease progress. These were: Yf, the final disease incidence; Y55, fruit incidence at day 55; Y95, fruit incidence at day 95; b and q, the relative and the absolute rate of disease progress, respectively; T1.5, the time when disease incidence reaches 1.5 %; M, the inflection point and AUDPC, area under disease progress curve. Descriptive disease variates were significantly different (P<0.05) for cv. Prima compared to cv. Idared, except for the relative and absolute rate of disease increase, b and q, respectively. The largest differences among cultivars were in the values of the AUDPC. Disease progress curves and descriptive disease variates were presented and the practical implications of the results were discussed.
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First results of varios sour cherry cultivar on Oblacsinska as interstocks and Prunus mahaleb rootstocks
87-90.Views:13865At the experiment orchard of the Fruit Research and Extension Institute, Újfehértó, Hungary in spring 2000 a trial was designed included: Erdi bőtermő, Debreceni bőtermő, and Csengődi sour cherry (Prunus avium L) cultivars which were budded at 1999 on Oblacsinska sour cherry (as a dwarf inter stock), which had been budded on Prunus mahaleb seedlings at 1998 with the aim to investigate the dwarfing effects of Oblacsinska sour cherry cultivars on scion cultivars. Trees of each cultivar directly on Prunus mahaleb seedling, severd as controls. Use of Oblacsinska as an interstock reduced the rootstock trunk diameter. Trees of Debreceni bőtermő, and Cseng6di sour cherry cultivars with Oblacsinska interstock were smaller than similar trees grafted directly onto Prunus mahaleb as a rootstock. Trees of Erdi bőtermő grafted on Oblacsinska interstock which buded on Prunus mahaleb were the smallest. Growth of the trees was reduced also by using of the Oblacsinszka as interstock for other examined cultivars.
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Bee pollination and association of apricot varieties
20-24.Views:1040Apricot yields are highly variable according to the season. The variation is caused mainly by the adversities during the critical processes of floral biology, i.e. blooming and fertilisation. On the basis of information concerning blooming time and mutual compatibility relations of apricot varieties a system of securing regular and adequate yields has been developed.
Winter frosts of the continental type are well tolerated by most of the apricots, however, after the end of rest period, flower buds are loosing frost tolerance, 'rapidly.
Being one of the fruit species blooming earliest during the early spring, apricot start to bloom in Hungary around the end of March or early April as a mean of many years, but it also happened, exceptionally that apricot started to bloom at February 20 (at Letenye South Hungary). The early season, exposes the floral organs to frost injuries. As a consequence, apricot orchards on the Great Plain produce low yields in 3 years, intermediate yields in other 3 years out of a ten-year-period.
Moreover, weather conditions during the blooming period are often unfavourable for pollination. Cool, windy and rainy weather prevents the flight of insects and on the other hand, warm spells shorten the blooming process, nectarines and stigmata get dry and the female gametes loose viability before effective pollination occurres.
The fertility of individual cultivars are meeting different obstacles. Apricot cultivars differ greatly in the rate of flowers bearing underdeveloped pistils, which may attain even 60% (e.g. Orangered). New commercial cultivars are often self-incompatible. Local varieties of that type in Hungary are the „óriás" varieties (e.g. Ceglédi óriás, Szegedi mammut), and the new hybrid Ceglédi Piroska. Many of the cultivars are variable in their self-fertility (partially self-fertile): Budapest, Harmat, Korai piros, Mandulakajszi.
Inter-incompatibility is also known in apricots. The „óriás " varieties do not fertilise each other. During the growth of fruits, cool spells (2-4 °C) caused severe fruit shed in Ceglédi óriás.
Apricot flowers produce pollen and nectar at average rates related to other fruit species, thus bees are attracted sufficiently. Bee visits are very variable according to growing site and season. Most of the bees are pollen gatherers but sometimes nectar suckers are in majority. Bee pollination is necessary not only for the self-incompatible varieties but also to enhance the yield of self-fertile varieties.
Taking the blooming and fertility relations of the cultivars into account, plantations should not exceed two rows to a particular self-incompatible varieties, and possibly two different polliniser varieties are suggested to be planted as flanking the block in question.
In commercial plantations 2 to 4 bee colonies per hectare are proposed to move for the whole blooming period.
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Erratic fruit set in almond under warm climates
59-64.Views:433The cause for the erratic yields of the two main almond cultivars grown in Morocco has been searched in order to recommend possible solutions for a high and stable production. The lack of sufficient bloom overlap between ‘Marcona’ and ‘Fournat de Brézenaud’ in many years may be one of the reasons of this erratic behavior, but not the only one, as the same behaviour may be observed in two simultaneously blooming cultivars, ‘Ferragnès’ and ‘Ferraduel’. The relatively high temperatures observed during the blooming period
significantly reduced the stigma receptivity of these cultivars and, as a consequence, their effective pollination period, showing that stigmatic receptivity is a limiting factor for fruit set in ‘Marcona’ and ‘Ferragnès’ and their subsequent yield reduction under warm conditions. Thus, in the present context of global warming, the search for cultivars tolerant to heat stress during flowering will acquire a special interest, as well as the combination of cultivars with the same chilling and heat requirements to ensure a simultaneous bloom. -
Effect of variety and cultivation technology on phenols and antioxidant activity of sweet and sour cherry
59-61.Views:1087The goal of the present work was to compare different sweet and sour cherry cultivars and cultivation methods (bio/integrated) with respect to polyphenol content and antioxidant activity. The concentration of total polyphenols ranged between 880–1050 mg kg-1 of fresh fruit, whereas antioxidant activity expressed as TEAC was found to be between 5.4 and 10.3 mmol kg-1 for the sweet cherry cultivars examined. In case of sour cherry the level of polyphenols ranged between 1283 and 3490 mg/kg fresh edible part of the fruit. Antioxidant activity was recorded between 15–32 mmol kg-1 for the different sour cherry cultivars included in this work. After one-month storage at low temperature, the total phenols and antioxidant activity decreased by 2–40% in the sour cherry cultivars studied. The anthocyanin content in cherry cultivars was less (131–312 mg kg-1) than the135–1893 mg kg-1 found in sour cherries. Anthocyanin level was higher in samples produced under organic farming conditions than in those produced with integrated cultivation.
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Antioxidant capacity, total phenolics and mineral element contents in fruits of Hungarian sour cherry cultivars
59-64.Views:1225Several epidemiological studies revealed that the consumption of antioxidant compounds and the risk of atherosclerosis, increased blood pressure or cancer are inversely proportional. Fruits of sour cherry contain a wide range of antioxidant compounds including melatonin, perillyl alcohol, ellagic acid, several flavonoids, polyphenolics, and anthocyanins. This study was carried out to survey the antioxidant power and mineral element content of seven commercial sour cherry cultivars and three cultivar candidates and to assess the influence of some external conditions on fruits' functional properties. Our analysis revealed nearly 5- and 2-fold differences between the lowest and highest antioxidant capacities and total phenolics content, respectively. Some cultivars (`Kántorjánosi' and ‘Újfehértói fürtös') and cultivar candidates (D, 'Petri' and 'Éva') showed outstanding antioxidant capacity and total phenolic content; in addition, mineral element content in fruits of the ‘Újfehértói fürtös' cultivar was also favourable. Redox parameters of fruits were influenced by the cultivation plot or fruit positions within the canopy in about half of the cultivars tested. Genetic background of cultivars forms the decisive factor in determining fruits' antioxidant capacity, although external factors may have also sizeable modifying effects. Enhanced functional properties of the fruit may also be further increased through breeding programs since considerable variation exists within the tested germplasm.
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RAPD analysis of grapevine hybrids and cultivars
63-66.Views:381Utilization of the Randomly Amplified Polymorphic DNA (RAPD) technique as a molecular marker was tested to investigate the relationships between some representative grapevine cultivars and hybrids established at the Department of Genetics and Plant Breeding (CUB), to distinguish clones as well as to characterize various hybrids between species or cultivars and their parents. Vitis vinifera cultivars were easily and successfully distinguished by the RAPD technique and they were grouped according to the traditional taxonomic classification. RAPD patterns of the examined Pinot gris clones proved to be completely identical. Number of generations was reflected by the value of genetic distance of the examined hybrids. Genetic identity of parents and their offsprings was influenced by the selection applied in the process of plant breeding. Parental phenotypic and morphologic characteristics showed high degree of segregation in hybrids, but RAPD analysis revealed that their genetic similarity is considerable. The three Vitis anntrensis clones were properly discriminated from every cultivar and hybrid of Vitis vinifera, i.e. hybrids are much closer to the cultivated grapevine than to V. anzurensis due to the phenotypic selection carried out during the life-cycle of one or two generations.
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Honeybee (Apis mellifera L.) visitation at the flowers of quince cultivars (Cydonia oblonga Mill.)
95-102.Views:763Studies were made on the bee visitation of 6 quince cultivars and on the foraging behaviour of honeybees at quince flowers for 3 consecutive years. The bee visitation was highly intense because both the plantation and its surrounding was overpopulated by honeybee colonies. Some 5.5 bee visits were counted in average at 50 opening quince flowers in 10 minutes and some 9.7 flowers were visited of the 50 ones observed meanwhile. This equals some 7 bee visits per flower per day per in good weather. Bee visitation, however, was variable and it was greatly different in the three consecutive years with fairly favourable weather. Some cultivars tended to be more and others less visited by honeybees than the others but these differences were not consequent at each occasion. No consequent relationship between the weather and the bee visitation of quince trees could be recognised. It was concluded that .other factors were responsible for the variable nature of the bee activity at quince. Most honeybees tended to collect pollen (51.6% in average for the 3 years) and usually much less gathered for nectar only (19.9%), and the rest of them were mixed behaviour foragers gathering for both (28.5%). There were some slight differences in the foraging behaviour of honeybees at some cultivars but these differences were not always consequent in consecutive years. Also the nectar production of flowers failed to affect the bee visitation and the foraging behaviour of honeybees definitely. For the variable nature of bee visitation and bee behaviour at quince flowers, supplementary pollination is needed to achieve as high set of fruits as high is required to a good crop at quince (at least 20-25% because the flower density is low of this fruit tree species). Since the intensity of bee visitation at the flowers is the only reliable estimate of the necessity of supplementary bee pollination further research is needed to explore the relationship between the number of honeybee visits and the consequent fruit set at quince.
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Incidence of postharvest decays on cultivars of pear, apricot, sour cherry and peach under two storage conditions
63-65.Views:18253In this two-year study, postharvest decays of pear, apricot, sour cherry and peach cultivars under two storage methods (TC and CA) were determined after four monthes storage periods; and then causal agents of postharvest decays of two pear cultvars were idenfified under traditional cold storage conditions. Results showed that postharvest decay was lower under controlled atmosphere compared to traditional cold one. Decay was lower on pear and the largest deacy occured on peach and apricot cultivars. Cultivars of fruit species also showed differences in incidence of fruit decays. Incidence of decays was independent on year effect. Under controlled atmosphere, postharvest decay ranged between 0 an 8% for pear, and between 5 and 12% for apricot, and between 6 and 11% for sour cherry, and between 5 and 15% for peach. Under traditional cold storage, postharvest decay ranged between 16 an 21% for pear, and between 15 and 39% for apricot, and between 10 and 22% for sour cherry, and between 19 and 33% for peach. Incidence of pear fruit damage ranged between 7.5 and 12.3%. Most damage started from injured fruit or wounded fruit. Five types of damage occurred ont he pear fruits in both years: Penicillium spp., Monilinia spp., Chondrostereum spp., other pathogens and mechanical injury. The most common damage was caused by Penicillium spp., Monilina spp. and Chondrostereum spp. On both pear cultivars in both years.
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Flower microphenology of Hungarian sour cherry cultivars in Iran climatical conditions
99-101.Views:1089Determination of flower microphenology for selecting the suitable pollinizer for sour cherry cultivars is of significant importance. In order to study the flower microphenology of Hungarian sour cherry (Bőtermő, Érdi jubileum and Cigány) cultivars in Mashhad climatic conditions, an experiment was conducted in I998. 1999, 2005 and 2006, using a completely randomized design with ten replications. The phenological stages were determined from before opening up to browning phase of stigma. Duration of pollen shedding, stigma receptivity and climatic factors were measured. The relative time of flowering between cultivars varied from year to year. The data indicate, the thermal variation strongly contributes to significant differences in duration of stigma viability, although there wasn't a significant difference between cultivars in anther dehiscence period. Phenological stages of flowers are highly affected by meteorological factors in various years.
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Susceptibility of European pear genotypes in a gene bank to pear psylla damage and possible exploitation of resistant varieties in organic farming
95-101.Views:1070We evaluated 285 pear genotypes (commercial cultivars, ancient local varieties, unnamed local strains, seedlings, wild seedlings) in the largest gene bank of pear in Hungary from the point of view of psylla resistance to explore their possible exploitation in organic farming. We have found some 10 new resistant types (Bókoló körte, Bôtermô Kálmán, Füge alakú körte, Nagyasszony körte, Nyári Kálmán, Rozs nyári körte, Viki körte, Pb-242, Pb-299, 0-632) and 7 highly tolerant ones (Cure-6, Kései Kálmán, Kieffer, Kieffer Éd, Steiner, Téli Kálmán, II. B-3- 6/4, 96-16/5) (Table 1). These made up 3.5 + 2.8 per cent of the investigated genotypes, while 93.7 per cent of them were susceptible to pear psylla damage. Taking earlier and present results into account we can list more than 30 European pear cultivars being resistant or highly tolerant to pear psylla infestation and damage. In fact, the list of resistant and highly tolerant cultivars may serve as a basis selecting pear cultivars fitting to the specific requirements of the organic farming. By the end we can conclude that there is some real hope to exploit some resistant or highly tolerant ancient or local cultivars in organic farming but further investigations are needed to estimate their yield capacity and fruit quality.
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Model experiments for establishment of in vitro culture by micrografting in apple
47-49.Views:477Micrografting was used in our experiments for establishment of in vitro culture from one rootstock (`JTE-F') and three scion cultivars (`Remo', 'Rewena' and `Reanda') of apple. Shoot tips of these cultivars were harvested from field and grafted onto in vitro rootstock cultivars. Their survival and development were studied. 42-93% of shoot tips survived and developed further depending on cultivar. Impermanent browning of sticking agar-agar could be observed in 21-25% of the micrografts depending on cultivars but discolouration of agar-agar ceased within one week and did not cause any death of shoot tips. We used micrografting successfully for establishment of in vitro culture from cultivars, from which earlier with conventional methods the culture establishment was not possible because of hard tissue browning. However, further studies are necessary to ensure the survival and development of shoots after removing them from micrografts.
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Nectar production of quince (Cydonia oblonga Mill.) cultivars
87-94.Views:1262Nectar production of six quince cultivars were measured during three consecutive years. The nectar production of quince can be regarded to be small compared to other temperate zone fruit tree species since quince flowers contained 1.07 ± 0.06 mg of nectar in average. The extreme values, however, ranged between 0.1 and 7.3 mg/flower and this indicated that the nectar production was highly variable. The distribution of the nectar production was definitely skew because low values were definitely much more frequent than the highest ones. Our findings do not corroborate the earlier statements on the high sugar concentration of quince nectar. We found some 21-27% sugar in average, only. The normal distribution of the sugar concentration also indicates that the typical sugar concentration may be between 20-30%. Accordingly, the sugar concentration of quince nectar is rather low compared to other temperate zone fruit tree species (except pear). There was a significant negative correlation between the amount of nectar and its sugar concentration in quince flowers in all of the three years of the study (r= -0.51, n=37, p<0.02 in 1996, r= -0.57, n=28, p<0.1 in 1997, r= -0.35, n=9 I, p<0.001 in 1998). No definite difference was established between the nectar production of quince cultivars. Nevertheless, one cultivar tended to produce less and two other ones produced somewhat more nectar in average than the rest of the 6 cvs investigated but the extreme values of nectar production of cultivars overlapped in most cages.
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Microsatellite based identification of grapevine cultivars traditional in Hungary and in the Carpathian Basin
71-73.Views:307Grapevine cultivars and clones traditional in Hungary and in the Carpathian Basin are maintained in the genetic collection of the Corvinus University of Budapest. Mostly ancient varieties and clones were genotyped using microsatellite loci. The investigated 6 loci were sufficient to distinguish all cultivars. Some clones could also be separated based mostly on the variable VVS2 loci. For 17 out of the 31 investigated cultivars this is the first report on characterization of the polymorphism of the allele lengths by microsatellite markers on loci: VVS2, VVMD7, VVMD27, vrZAG62.
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Freeze Susceptibility of Fruit Buds in 67 Apple cultivars in Hungary
29-35.Views:235Frost damage is one of the most important risks of apple production. Outstanding importance has been attributed to the frost resistance of flower-buds as decisive sites of fruit production. Browning of plants parts and tissues exposed to natural weather adversities are considered as effects of frost. In Hungary, frost damage on flower buds of both the market and new cultivars has not been assessed earlier. Observations referring to the consequences of frost damages of over four critical years, marked by their peculiar winter and spring frost hazards. Parallel observations have been made in four sites of the growing area in the Great-Plain region. 67 apple cultivars have been assessed. Each cultivar was represented by 3 trees, which were sampled at about 1-1.5 m height over ground, where the buds or inflorescences were picked for the purpose to assess the injury. The buds and flowers are cut longitudinally and rated visually according to the extent of browning of the organs and tissues. Susceptibility of different organs of the flower (pistils and anthers) were rated separately. According to our results, most resistant to spring frosts are the following cultivars: 'Gloster', 'Granny Smith' and appreciable tolerance is attributed in 'Gala' and 'Jonathan' with derivatives. Preliminary results that among the scab resistant cultivars, `Baujade', 'Rewena', 'Liberty', `Resi' and 'Renora' are rather frost resistant. Information, lacking hitherto, is obtained upon 'Reka' and 'Reglindis' as for their increased susceptibility.
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Determination of the cold tolerance off sour cherry cultivars with frost treatments in climatic chamber
49-54.Views:834Nowadays, sour cherry buds can be seriously damaged by spring and winter frosts. Unlike other fruit species threatened by high frost damage, sour cherry cultivars have not been assessed for frost tolerance. The aim of .our survey was to establish the relative cold tolerance of the Hungarian cultivars after treatment in a climatic chamber, and to optimize the methodology formerly elaborated for the frost treatment of apricot. Fourteen cultivars of Hungarian sour cherry (Prunus cerasus) were used in the experiments, which spanned the winters of 2005/2006 and 2006/2007. Our data were used to rank cultivars in two groups according to their levels of cold resistance. We also recommend critical temperatures and treatment times for the testing of sour cherry cultivar resistance to cold in climatic chambers.
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Genotypic difference of garlic (Allium sativum L.) cultivars for growth, bulb yield and yield related attributes at Tigray Province, Northern Ethiopia
46-52.Views:350The experiment was conducted at Ofla district from June to October of 2017 and 2018 cropping season to select high yielding and adaptable garlic cultivar(s). Six garlic cultivars namely Chefe, Tsedey, Holeta local, Kuriftu and Bora-4 and one Ofla Local were evaluated. The experiment was arranged in completely randomized block design (RCBD) with three replications. Different growth, phenology, yield and yield related data were collected. Leaf length, leaf width, bulb diameter and length were significantly (p<0.05) influenced by garlic cultivars in both cropping seasons. Bulb weight and number of cloves per bulb were highly significantly (P<0.01) influenced by cultivars in both cropping seasons. Also, marketable yield, unmarketable yield and total yield ha-1 were highly significantly (P<0.01) influenced by cultivars in both seasons. During 2017 and 2018, the highest marketable bulb yield was observed from Ofla Local cultivar with an average value of (8.86 t/ha) and (9.06 t/ha) respectively. During the 2017 cropping year, the maximum and significantly higher total bulb yield of 10.21 t/ha was recorded on the Ofla Local cultivar. Moreover, in 2018, this cultivar provided the maximum total bulb yield of 9.80 t/ha. Therefore, the Ofla Local cultivar showed the greatest performance for growth, yield and yield related attributes. Thus, it is recommended for cultivation in areas having similar agro-ecology. To improve the productivity of garlic, it is important to study and identify the optimum fertilizer level and spacing in the study area.
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Susceptibility of sour cherry cultivars to isolates of Monilia laxa (Ehrenbergh) Saccardo et Voglino
83-87.Views:496In this study, the susceptibility of 7 commercially important sour cherry cultivars to Monilinia laxa was studied. Artificial inoculation was made with M. laxa isolates, which were isolated from different woody plants. Artificial inoculation was prepared in the laboratory and in the field. In laboratory, flowers of sour cherries while in the field, the two-year old twigs were inoculated in 2006 and 2007. According to results of stigmata inoculation, there were infection ability differences among the isolates originated from five different stone fruit host. Cultivars could be sorted into two susceptibility groups. In the field, twig inoculation in 2007 was made at blossom period and in 2007 at harvest. Seven sour cherry cultivars were inoculated with 8-day-old mycelial culture of M. laxa originated from sour cherry and almond. The agressivity and pathogenicity of the two isolates were measured by the degree of floem death: Results showed that year and phenological stage considerably influenced the degree of symptoms caused by the fungus. After artificial inoculation, tissue death progression was studied by fluorescent microscope. According to results, sour cherry cultivars were sorted into disease susceptibility groups. Susceptibility orders were identical to results on stigmata inoculation.
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Antioxidant capacity and total polyphenol content of Lavandula cultivars at different growing areas in Hungary
65-69.Views:555Lavandula species are worldwide grown essential oil producing medicinal plants with considerable economic value. Beside volatile oil, lavender also contains different phenolic compounds which have been less widely studied till now. Cultivation of lavender has become widespread in Hungary in the recent years, however, growers have limited knowledge on the productivity of cultivars available. In our research we were aimed to studying the antioxidant capacity and total polyphenol content of samples collected in two growing areas (Dörgicse and Szomód) from flowers of two L. angustifolia (‘Hidcote’, ‘Munstead’) and two L. × intermedia (‘Grosso’, Grappenhall’) varieties, during the full blooming period of 2017. Antioxidant capacity of the samples was determined by FRAP assay (Benzie and Strain, 1996), while total polyphenol content (TPC) was measured by a modified method of Singleton and Rossi (1965). According to our results, varieties of L. × intermedia showed higher values of antioxidant capacity and of total polyphenol content, than those of L. angustifolia cultivars. Among them, both the antioxidant capacity (179.6 mg AAE/g DW) and total polyphenol content values (152.4 mg GAE/g DW) of ’Grosso’ from Dörgicse were the highest. Concerning FRAP values of all the cultivars investigated, larger variability were found in Dörgicse, than in Szomód. Regarding the effect of growing area, each cultivar represented similar FRAP values in Dörgicse and in Szomód, except for ’Grosso’. However, in the case of TPC values, higher variability was observed between the growing areas, especially in the case of ’Grosso’. In the future our studies on lavender polyphenols will be completed with qualitative evaluation of the values obtained by HPLC analysis.
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The effect of modified bacterial virulence to host-pathogen relationship (Phaseolus vulgaris L. Pseudomonas savastanoi pv. phaseolicola)
53-56.Views:552The Pseudomonas savastanoi pv. phaseolicola is one of the most expressive biogen stressors of the bean (Phaseolus vulgaris L.) in Hungary. The chemical and agrotechnological defence is inefficient, so breeding is the only workable way. The conventional cultivars are susceptible to PS while most of the new industrial varieties have genetic resistance to the pathogen. The genetic background of resistance is, however, a complex system in the bean. Leaf resistance is a monogenic system, but this gene is not expressed in juvenile stage of the host. The pathogen species can be divided into different races. After inoculation with virulent strains, typical symptoms appeared on the leaves. To understand the details of host-pathogen relationships, there were carried out experiments using bacterial strains with altered virulence. Six transposon mutants of the PS were tested. Our main objective was to test these modified bacterial strains on bean cultivars of known genetic background. First we analysed the symptoms, and then the correlation between the symptoms and the multiplication of mutant bacteria. Three cultivars (Cherokee, Inka and Főnix) were tested.
The infection by the virulent PS isolate produced typical symptoms on the three cultivars tested. Mutant bacteria (except strain 756) did not cause any significant symptoms on the hosts. The mutant 756 induced visible symptoms on the cultivars Cherokee and Inka. On Cherokee there were small watersoaked lesions, and HR (hypersensitivity reaction) was detected on Inka, but this was restricted to some cells only (mikro HR). The rate of multiplication of the wild type strain was much higher than the multiplication of the mutants. Bacteria were detected in the cotyledons and primordial leaf, but there is not any substantial number of bacteria in leaves, except for strains 757, 1212 and 1213. The rate of multiplication of strain 756 was intermediate. These, and other experiments can help to understand the genetic background of resistance and the host-pathogen relationship in the Pseudomonas-bean pathosystem.
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Biological performance of grape varieties in Eastern Hungary
31-35.Views:380Eurasian and interspecific grape varieties and candidates were inspected for biological productivity in the variety collection of the University of Debrecen at the Horticultural Experimental Station in Pallag. In this paper, data are reported on yields (kg/stock), cane production (kg/ stock) and use-up index of wood yield of each cultivar in the experimental years 2011-2012. From the range of Eurasian (Vitis vinifera L.) white wine-grape qualified cultivars and candidates ’Ezerfürtû’, ’Generosa’, ’Jubileum 75’, ’Müller Thurgau’, ’Zöld veltelíni’,’B-11’, ’CSFT-92’, ’Pintes’, from the range of Eurasian (Vitis vinifera L.) red wine-grape qualified cultivars and candidates ’Kármin’, ’Pinot noir’, ’Alicante Bouschet’ and ’Rubintos’ were highlighted. Promising data of interspecific ’Aletta’, ’Csillám’, ’Kunleány’, ’Orpheus’, ’Refrén’, ’Taurus’, ’Viktória gyöngye’, ’Alföld 100’, ’Reform’, ’RF38/32’, ’Toldi’ white, and ’Dunagyöngye’ ’Pannon frankos’ red wine-grape cultivars and candidates were emphasized. Published data refer only to the biological performance of the cultivars. The thorough evaluation of varieties needs respect to data on resistance to fungal disease and climatic extremities, and other characteristics concerning production technology and oenological parameters.
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Comparative evaluation of chamomile (Matricaria recutita L.) populations from different origin
91-95.Views:668In our research project under cultivation we examined 4 cultivars ('Soroksári 40', 'Lutea , 'Goral', 'Bona') and 28 wild populations of chamomile assuring all of them the same environmental conditions. Plant height, flower-diameter, essential-oil content, and the main terpenoid and flavonoid composition were analysed. The aim of our study was to establish the genetic background of breeding a new cultivar as well as encircle those natural habitats that give chamomile drug with the best quality.
In the case of plant height populations from the Great Hungarian Plain were lower than plants from Transdanubia and the control cultivars. Between the wild and the standard individuals we found significant differences with relevance to the flower-diameter. With regard to the essential oil content the populations were very heterogeneous even those, which came from the same habitat. Populations form Transdanubia and Nagyiván reached the essential oil level of the cultivars (0.721-0.931 gi100g), and 75% of the examined plants exceeded the minimum requirement of the PhHg VIII.
According to the essential oil composition our previous statement was confirmed that in the populations of Transdanubia and Northern part of Danube—Tisza Mid Region the main component is bisabolol-oxide A (30-41.2%), while plants native to the territory cast of the river Tisza are mainly characterised by a-bisabolol (32.3-48.4). In some samples the ratio of bisabolol-oxide B was more than 10%. The chamazulene content was higher in the cultivars selected to this component (above 20%), than in the wild populations (varied between 1.22 and 17.2%). Populations originated from the central part of Hortobágy region had extremely high apigenin content (10-13 mg/g), but in the case of chlorogenic acid, hyperoside and quercitrin we did not find any differences affected by the origin.
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Comparative analysis of apricot cultivars based on their ecological and biological indicators
35-50.Views:458The herbaceous plants organic characterize Ellenberg et al. worked out (1991), well-use system, which is updated with herbaceous and woody plant in the Hungarian flora species, so Soó (1964-1985), Zólyomi et al. (1967), Précsényi (1986) and Simon (1988) also addressed by different aspects of this problem circuits. The author is the first extended-Borhidi –Ellenberg’s system of wild fruit species (Surányi 2000, 2006) and cultivated of fruit (Surányi 2014) as well. Additional considerations there were aspects of the study of fruit varieties, these biological indicators following open pollination, frost tolerance, resistance of Sharka virus and disease susceptibility for. Firstly, we introduced a system for improving it a plum species and cultivars (Surányi 2015). In this case we used the new system among species and varieties of apricots, because diversity was able to express significantly. Especially the SB, WB, NB, and the relative biological value figures showed the variety. RB (reaction figures) fluctuated only slightly among the 463 varieties, but the dynamic difference between the 11’s was an indicator for the characterization of apricots. If the comparison performed plum and apricot variety’s level anyway justified the use of 11 kinds of organic and biological indicators.
