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Evaluation of Colour Versions of Wild Sage (Salvia nemorosa L.)
111-115.Views:216In the continental weather zone, more and more frequently occurring extreme conditions require continuous renewal of the market which generates constant challenge for the ornamental plant breeders. Most of the traditionally used decorative ornamental plants are sensitive to these extreme conditions. In 2001, Department of Plant Biotechnology, Debrecen University initiated an interdisciplinary breeding program in collaborations with Zoltan Kovats (he dealt with hungarian drought-tolerant plant species) to produce new or reintroduce forgotten drought-tolerant ornamental species into public parks and roadsides. From ~900 species of Salvia genus, Salvia nemorosa L. has been known as a medical plant, however, because of its high adaptation ability and decorative nature it is a highly recommended ornamental plant as well. Salvia nemorosa L. has a low maintenance, extremely droughttolerant, fast growing plant, generates proper cover, and highly competing weeds on roadsides. Nowadays, 50-60 varieties are available; however this number could be increased by new hybrids. Great morphological and colour variation could be seen within the species, from different white to deep violet. The main goal of this research is the production of elite lines with wide colour and morphological variation in wild sage. We have already obtained 25 different clones for further investigation without eliminating the original plants generating an in vitro gene bank as it has been done by Italian breeders.
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Evaluation of crop yield and fruit quality in organic apple production
7-10.Views:226In this study, crop yield and fruit quality parameters (soluble solid concentration, sugar, total acid, and vitamin C content) of 15 apple cultivars including old, resistant and currently grown cultivars. The highest fruit yield among all 15 cultivars was obtained on CV. Jonagold with 15.5 kg/tree, while the lowest yield was measured on the resistant cultivar Reanda with 8.1 kg/tree. There were no significant differences among the cultivars in fruit diameter ranging from 70 mm in the case of cv. Húsvéti rozmaring to 82 mm for cv. Mutsu. Values of soluble solids concentration of the cultivars varied between 15.5% for cv. Téli Banán and 19.2% for cv. Renora. Sugar content values showed a similar tendency to soluble solids content. The highest and lowest total sugar content was observed on cvs. Jonagold and Renora and cv. Retina with 17% and 12.4%, respectively. Total acid content values ranged between 0.18% and 0.53% for cvs. Jonagold and Remo, respectively. Values of vitamin C content for the 15 apple cultivars varied between 2 mg% and 4 mg% for cv. Retina and cv. Mutsu, respectively.
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Prevalence of banana diseases and post-harvest losses in Kenya, and biocontrol potential of arbuscular mycorrhizal fungi against Fusarium wilt
62-73.Views:254The demand for bananas (Musa spp.), which is ranked as the most important fruit crop in Kenya has been on the rise owing to both their dietary contribution and income generation. Meeting this demand has however been hampered by losses during production or post-harvest. This study assessed banana disease and post-harvest losses in leading producing counties in Kenya namely; Kisii, Nyamira and Embu. The study also assessed the efficacy of Rhizophagus irregularis in controlling Fusarium oxysporum f.sp. cubense. Structured questionnaires were used to collect data on post-harvest losses. Disease scoring tables, charts and photos were used to confirm observed symptoms and hence, disease occurrence and severity. AMF biocontrol efficacy experiment was conducted using tissue culture bananas grown in the greenhouse. The study revealed that most smallholder farmers were unaware of the causes or the prevalence of post-harvest losses. The findings also revealed a significant difference (p<0.05) in the severity of banana diseases across various cultivars from the three counties. The AMF treated bananas showed a significant difference (p<0.05) in plant height, total leaf area and chlorosis in comparison to other treatments. The study also revealed a reduction of Fusarium’s pathogenic effects including chlorosis, reduced leaf surface area and eventual necrosis.
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Adaption of temperate climate horticultural plants in tropical and subtropical developing countries (Review Article)
7-11.Views:257Adaption of temperate climate horticultural plants in tropical and subtropical developing countries (Review Article)
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Comparative study of sour cherry samples for their anthocyanin content measured by tools of the laboratory and by portable equipments
29-31.Views:305The inner quality of fruits depends on many components. The analysis of pharmacologically important fruit species as the sour cherry consumed as a common food is an expensive task, mostly requiring an equipped laboratory. In sour cherry, one of its valuable components, the anthocyanin content deserves special attention. A portable analytical implement, which has been developed by our team measures the anthocyanin content on a scale of 1024 PharMgrades. The system is a member of the UVEX family of implements working with a microprocessor and performs the measurement quickly. The portable variant (UVEX-ML-1) does not require much training, the laboratory variant (UVEX UL-1) performs a large quantity of tests under modest laboratory conditions. Preparation of the samples is easily done, the necessary elements are available in the trade. The reagent is easily stored and dosed. The system was tested and proved to be of sufficient precision and the result showed acceptable variance according to the checks performed by a spectrophotometer in the laboratory.
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Primers designed for the detection of grapevine pathogens spreading with propagating material by quantitative real-time PCR
21-30.Views:291Several grapevine pathogens are disseminated by propagating material as systemic, but latent infections. Their detection and identification have a basic importance in the production and handling of propagating stocks. Thus several sensitive and reliable diagnostic protocols mostly based on molecular techniques have been developed. Of these methods quantitative real-time PCR (q-PCR) has recently got an emerging importance. Here we collected primer data for the detection and identification of grapevine pathogens which are important in the production of propagating stocks by q-PCR. Additional novel techniques that use DNA amplification, hybridization and sequencing are also briefly reviewed.
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Agrobacterium transformation of Rhodiola sp.: current status and limitations
37-42.Views:80The study of secondary metabolites has led to the discovery of new drugs for treating human diseases. However, consistent plant supply can be challenging, leading to the use of plant tissue culture techniques such as hairy root culture. Hairy roots have stable genetics, lateral branching, and can produce secondary metabolites, including alkaloids, flavonoids, and terpenoids. Research on hairy roots as a subject began in the late 19th century, and for the last four decades, hairy roots have been utilized for producing secondary metabolites and recombinant proteins. This article focuses on Rhodiola species - genus of perennial plants that belongs to the family Crassulaceae - and its potential as a source of secondary metabolites using hairy root culture techniques. Rhodiola sp. is widely distributed throughout the Arctic regions of the Northern Hemisphere, with several species having significant medicinal properties. The article discusses the possible use of hairy root cultures for the production of Rhodiola secondary metabolites, including salidroside and rosavins, which have demonstrated significant pharmacological activity in various studies. The use of elicitation and genetic engineering techniques to boost secondary metabolite production in Rhodiola hairy roots is also explored. Overall, the article highlights the potential of Rhodiola hairy root cultures as a valuable source of secondary metabolites with medicinal properties. However, despite some studies Rhodiola hairy root induction and culturing still remains highly unexplored.
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Field evaluations of 14 sweet cherry cultivars as pollinators for cv. Regina (Prunus avium, L.)
75-77.Views:270In this study, the pollen of 14 sweet cherry cultivars (‘Anella’, ‘Duroni 3', 'Badacsony', 'Cristalina', 'Ferbolus', 'Ferrovia',
'Georgia', 'Hudson', 'Kordia', 'Sam', 'Schneiders’, ‘Spate’, ‘Knorpelkirsche', 'Skeena', 'Summit', 'Sylvia') was used to fertilize the emasculated flowers of sweet cherry cv. 'Regina'. Fruit set was assessed three times during fruit development: 14 May, 30 May and 27 May 2007. We observed full incompatibility among the 14 cultivars for cv. 'Cristalina', which is in the same S-allele group as cv. 'Regina'. After analysis of our data, we have results about fertilization efficiency of the cultivars. Most of the evaluated cultivars are inadequate to fertilize cv. 'Regina' to a sufficient degree. There were two exceptions, cv. 'Sam' and cv. 'Skeena', where percentage of ripened fruits was above 20%. These two cultivars can guarantee such a pollination, which ensures ample quantity of ripened fruits. Results of this study have proved three other cultivars to be quite good pollinators for cv. 'Regina'. In conclusion, ideal pollinators for cv. 'Regina' could be — apart from above-mentioned two cultivars, 'Sam' and 'Skeena' — cvs. 'Sylvia' and 'Bianca', which was suggested by more literature sources.
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Effects of methyl jasmonate, salicylic acid and phenylalanine on aloe emodin and aloin in diploid and tetraploid Aloe barbadensis
Views:253Aloe vera is one of the most famous medicinal plants. Aloin and aloe emodin are the most important active compounds in this plant. The purpose of this research was the comparison of aloin and aloe emodin production after the elicitation by methyl jasmonate, salicylic acid, and phenylalanine in diploid and tetraploid Aloe vera plants in greenhouse conditions. The plants were treated with the concentrations of 25, 50, and 100 µM. The amounts of aloin and aloe emodin were determined 24 and 48 hours after application of the treatment. HPLC analysis showed that the leaves of the control diploid plants (without applying elicitors) had more aloin (1.20 fold) and aloe emodin (1.14 fold) than the control tetraploid plants. The maximum concentration of aloin (1.15 ± 0.07 µg mg-1 dry weight) was obtained after the elicitation by 25 µM methyl jasmonate, 24 hours after treatment, in diploid plants) 6.36 fold compared to the control (0.18 µg mg-1 dry weight (. In addition, the maximum concentration of aloe emodin (0.28 µg mg-1 dry weight) was obtained after the elicitation by 25 µM salicylic acid, 24 hours after treatment, in diploid plants) 6.18 fold compared to the control (0.04 µg mg-1 dry weight)). The long-term effect of three studied elicitors (after 240 days) on plant health and survival was also studied. This investigation showed that only methyl jasmonate at a concentration of 100 µM was resulted in the death of Aloe vera plants.
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Genetic diversity in a collection of apple (Malus x domestica Borkh.) cultivars as revealed by RAPD markers
25-35.Views:187A collection of 151 apple cultivars was investigated with 7 RAPD primers generating 71 informative bands, to evaluate genetic variability and relatedness. All cultivars presumably derived through genetic recombination were distinguished whereas identical DNA profiles indicated that some cultivars had arisen as sports. A cluster analysis and a PCO did not reveal any distinct geographic patterns, but there was a weak tendency for Swedish and foreign cultivars to differentiate.. Many cultivars however clustered together with either one of their parents or with siblings. Overall genetic diversity among the 151 cultivars was estimated with Nei's diversity index (H), 0.269, and with Shannon's index (H'), 0.594. The cultivars were also analysed in six groups, according to time of origination and country of origin, with an average H = 0.262 and H' = 0.546. No major differences in genetic diversity were observed over time or space, although the group with recent, foreign cultivars had the lowest diversity (FL = 0.235, H' = 0.493). Comparison between the entire material and a subset with 94 mandate cultivars chosen for preservation in Sweden, showed similar genetic diversity: HFN.rj1E = 0.268, frE,NTIRE, = 0.593 and HMANDATE = 0.263, WMANDATE = 0.575. No major differences in band frequencies were observed between these two sets, but 5 RAPD bands were missing in the set with mandate cultivars.
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Genetic engineering of apple (Malus domestica Borkh.) for resistance to fungal diseases using g2ps1 gene from Gerbera hybrida (Asteraceae)
15-12.Views:291In the present study, g2ps1 gene from Gerbera hybrida coding for 2-pyrone synthase which contribute for fungal and insect resistance was used. The aim was to work out an efficient approach of genetic transformation for apple cvs. ‘Golden Delicious’, ‘Royal Gala’ and ‘MM111’, ‘M26’ rootstocks for improving their fungal resistance using genetic engineering techniques. Adventitious shoot formation from leaf pieces of apples studied was achieved using middle leaf segments taken from the youngest leaves from in vitro-grown plants.
Optimum conditions for ‚direct’ shoot organogenesis resulted in high regeneration efficiency of 0%, 95%, 92%, 94% in the studied apples respectively. Putative transgenic shoots could be obtained on MS media with B5 Vitamins, 5.0 mg l-1 BAP, or 2.0 mg l-1 TDZ with 0.2 mg l-1 NAA in the presence of the selection agent “PPT” at 3.0-5.0 mgl-1. Shoot multiplication of transgenic shoots was achieved on: MS + B5 vitamins + 1.0 mg l-1 BAP + 0.3 mg l-1 IBA, 0.2 mg l-1 GA3+1.0 g/l MES+ 30 g/l sucrose + 7.0 g/l Agar, with the selection agent PPT at 5.0 mg l-1 and were subcultured every 4 weeks in order to get sufficient material to confirm transformation of the putative shoots obtained. Six, seven, one and six transgenic clones of the apples studied respectively have been obtained and confirmed by selection on the media containing the selection agent “PPT” and by PCR analysis using the suitable primers in all clones obtained for the presence of the selection” bar gene (447 bp) and the gene-of- interest “g2PS1” (1244 bp), with transformation efficiency of 0.4%, 0.6%, 0.1% and 0.3% respectively. These transgenic clones were multiplied further in vitro in the presence of the selection agent ‘PPT’ and rooted in vitro. Rooted transgenic plantlets were successfully acclimatized and are being kept under-containment conditions according to the biosafety by-law in Syria to evaluate their performance for fungal resistance . -
Irrigation Requirements for Grape Crop under Climate Changes Conditions in Egypt
23-27.Views:260The present work is mainly directed to discuss sensitivity of climate changes upon the irrigation demand for grape crop in Egypt. The Penman Monteith equation was used to calculate reference Evapotranspiration (ETo) under current and future climate for the two locations (El Menya and El Beheira). The historical climate data for ten years from (2000 – 2010) was used as current climate to calculate irrigation requirement for grape crop under Egyptian conditions. Two climate changes scenarios have been applied as changes in temperature. The first scenario supposed that increasing in temperature of 1.5°C would happen, and the second scenario supposed that increasing of 3.5°C would happen to calculate reference Evapotranspiration and irrigation requirement for future climate. The results showed that the evapotranspiration and irrigation requirement for grape crop at El Menya location higher than El Beheira location. Irrigation demand for grape plant under two climate changes scenario will increase in El Menya and El Beheira locations. El Menya location will take the highest irrigation demand under climate changes. Therefore, possible adaptation countermeasures should be developed to mitigate the negative effects of climate changes for the sustainable development of agro-ecosystems in Egypt.
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Total anthocyanine content and antioxidant density of some Hungarian sour cherry varieties
109-111.Views:435Evidence suggests that a diet high in fruits and vegetables may decrease the risk of chronic diseases, such as cardiovascular disease and cancer, and phytochemicals including phenolics, flavonoids and carotenoids from fruits and vegetables may play a key role in reducing chronic disease risk. Recent research has proven that sour cherry (Prunus cerasus L.) is a valuable natural source of some bioactive compounds important in human health preservation. In our work, we identified the total antioxidant activity, anthocyanine content and "antioxidant density" of sour cherry varieties named "Újfehértói fürtös", "Debreceni bőtermő", "Kántorjánosi" and "Érdi bőtermő" and those of the "Bosnyák" sour cherry clones. "Antioxidant density" is a biological value indicator obtained in a synthetic way, which indicates the antioxidant capacity of the particular food, e. g. fruit and vegetable, related to 1 Calorie.
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Luminescence variations in cucumber (Cucumis sativus L.) leaves derived from different regeneration systems
50-52.Views:164Plants obtained from in vitro culture can show increased susceptibility to environmental stress conditions. In the process of their adaptation to natural conditions it requires monitoring of their physiological state. The methods used to check this phenomenon should estimate quickly and exactly the tolerance to suboptimal environmental factors. Such requirements are satisfied by the methods of measuring chlorophyll luminescence in vivo, e.g. fluorescence induction and delayed luminescence. The objects of our studies were cucumber plants regenerated from cultures of callus and embryogenic cell suspension, as well as the plants obtained from seeds. The plants derived from in vitro cultures displayed a poor physiological condition at the early phase of adaptation characterised by higher susceptibility both to stress caused by increased density of the light flux and low temperature (4 °C) in comparison with the plants obtained from seeds.
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Study on the viruses of Petunia in Hungary
55-59.Views:149Virus susceptibility of 11 varieties of vegetatively propagated petunia against 2 virus strains (TMV-C/U1 and PVYNTN) were examined. Neither resistant nor tolerant varieties of petunia were found. The virus infection of the Petunia genus was examined in Hungary. The most common pathogens were the Tobacco mosaic tobamovirus (TMV) and the Tomato mosaic tobamovirus (ToMV), but the samples also contained Alfalfa mosaic alfamovirus (AMV), Cucumber mosaic ClIC11171014171S (CMV) and Potato Y potyvirus (PVY). Potato X potexvirus (PVX) was isolated in the varieties of trailing petunia for the first time.
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Effect of three storage methods on fruit decay and brown rot of apple
55-57.Views:623The aim of our two-year study was to evaluate fruit decay and Monilinia fruit rot in three controlled atmospheres (CA), ultra-low oxygen (ULO) and traditional storage methods on apples for a duration of several months storage period. Four phytopathological treatments were studied under each storage condition: 1) 48 healthy fruit per unit, 2) 48 injured fruit per unit, 3) 47 healthy fruit and 1 brown rotted fruit per unit, and 4) 47 injured fruit and 1 brown rotted fruit per unit. Our results clearly demonstrated that fruit loss during storage is highly influenced by storage conditions and health status of the stored fruits. In the 2005 experiment, the lowest and largest fruit decay occurred under the ULO and traditional storage conditions, respectively (Table 1). The fruit decay was significantly different for the different storage methods. Fruit decay was fully suppressed in ULO storage except in the treatments of injured and injured + 1 brown rotted apple. Under CA and traditional storage conditions, when healthy fruit was stored, fruit decay was significantly lower compared with injured fruit including 1 brown rotted fruits. However, half of the fruit decay was caused by M. fructigena in CA store irrespective to phytopathogenic treatments. In 2006, results were not so consistent on cv. Idared but were not essentially different from the 2005 experiments.
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Innovative research of ornamental plants in University of Debrecen (2001–2014)
111-117.Views:157In the University of Debrecen started in 2001 related and biotechnology, genetic researches on climate-changerelated.
This work has already visible signs on the city’s public spaces. The main goal of participants of the program is it that
in Debrecen and in the surrounding communities more adaptable to the variable climate, ecologically and biologically more
grounded urban ornamental planting are preferred in the next decade. The new results and ongoing researches of the decorative
ornamental and biomass plants are an interdisciplinary breeding program. -
Influence of environment in electro-chemical quality of tomato
17-20.Views:191The aim of the research was to investigate the impact of different cultivation environments of tomato cultivars, the electro-chemical and the qualitative traits of the tomato fruits. The quality of tomato fruit harvesting was evaluated at the Laboratory of Institute of Horticulture and Viticulture University of Natural Resources and Applied Life Sciences, Vienna. Two hybrids of tomatoes were tested Belle-F1 and AmaF1, in two locations and two different treatments (open field and plastic tunnels). Model of experimental fields was based on random method and included three replications. Following parameters were analyzed and tested: pH, Rh, P-Value, Nitrates and the content of vitamin C. The values of pH for treatment in the open field and indoor production system were not significant (4.47 to 5.05). For P-Value derived from the two treatments the maximal values were without significant differences, while minimal values were significant. Study has shown also that the highest values of nitrate content (16.34 mg, open field) compared to average value differences were + 0.384 mg or 43.83% higher. In indoor production the nitrate content was lower in compared with plants cultivated in open fields, so these differences were +3.81 mg or 26.4 0%, and highly significant for both levels. In relation to vitamin C content, the highest value was identified in the indoor systemof 298.6mg, with variations from the overall average of 16.43% or + 42.15 mg. The coefficient of variation values for both levels was of 29.96 respectively 27.31%.
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Prediction infection risk on the basis of weather-related factors and Erwinia amylovora colonization in apple and pear flowers
39-54.Views:170Current infection risk prediction models utilize environmental parameters and field records, but do not take into account the estimated inoculum potential within the orchard. The object of this study was to survey the accuracy of three simple prediction methods under Hungarian climatic conditions, which could easily be used by the farmers. We also tested whether the accuracy of infection risk predictions can be improved by taking into consideration the incidence and/or rate of flower colonization by Erwinia amylovora.
After preliminary investigations in 1999-2001, data concerning the weather-related infection risk were recorded in 5 apple and 1 pear orchards in 2002, and in 12 apple and I pear orchards in 2003. The weather data were processed by the easy-to-use risk assessment models of the mean temperature prediction line (MTL), Smith's Cougarblight 98C and Billing's integrated system (BIS), and by the MaryblytTM 4.3 computer-assisted model for reference. The population size of E. amylovora in the flower samples was estimated within an order of magnitude by PCR.
For all years and orchards tested, Maryblyt indicated 35 days on which there was an acute infection risk. The same days were indicated by all 3 methods in 23 cases (66%), 8 days were indicated by 2 methods (23%) and 4 days were indicated by 1 method only. A similarly good correlation was found for prediction of the date of the first massive infection risk: in 2003, for instance, there was a perfectly consistent prediction by all 4 models in 9 of the 13 participating orchards. A coincidental forecast was provided by 3 of the 4 models in the other 4 orchards.
The results indicate that any of the risk assessment models could provide an increased accuracy of the actual infection risk prediction if combined with an estimation of the incidence of Erwinia amylovora colonization in the open flowers. We found no convincing differences in the size of the epiphytic population in flowers of cultivars possessing high or low susceptibility to Erwinia amylovora.
We conclude that the easy-to-use methods tested could be used by the fanners to recognize weather-related risks, especially when coupled with an estimation of the proportion of the pathogen-infested flowers. This local prediction would provide rapid information (faster than the regional forecast systems) specifically for a given orchard.
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Yielding capacity and regeneration of different ages fruit-bearing shoots in sour cherry
55-57.Views:187The yielding potential and regeneration (i.e. germination of hidden buds) after pruning of the fruit-bearing shoots of different ages were compared in sour cherry cultivars pruned to spindle shape. The examinations were carried out in order to determine the optimal pruning strategy resulting in the highest long-term yielding of sour cherry cultivars with intensive crown shape (free and thin spindle shape). According to our results, there are great differences among the studied self-fertile sour cherry cultivars in yielding and regeneration, which are worthwhile to be considered in determining the method and degree of pruning.
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Conventional PCR primers for the detection of grapevine pathogens disseminated by propagating material
69-80.Views:307Polymerase chain reaction driven by sequence specific primers has become the most widely used diagnostic method to detect and identify plant pathogens. The sensitive and cost-effective pathogen detection is exceptionally important in the production of propagating material. In this paper we have collected primer sequence data from the literature for the detection of the most important grapevine pathogens disseminated by propagating stocks by conventional polymerase chain reaction. Basic protocols to obtain template nucleic acids have also been briefly rewieved.
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A review of the orchard management in organic fruit production
43-49.Views:238The elements of orchard management have a determining role in the success of organic fruit production, therefore, it is essential that they are applied properly. The present review gives describes some aspects of the orchard management such as site selection, orchard soil, rootstocks and cultivars, planting materials and plant density, crown shape. Then, one of the most important elements, pruning is discussed with special emphasis on winter pruning, condition and apical dominance, degree and method of pruning, summer pruning. After pruning, an overview of fruit thinning, irrigation, soil cultivation, soil protection (cover plants and mulching) and fertilization is given.
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Some structural characteristics of the flowers of apple cultivars with different susceptibilty to fire blight
21-23.Views:127Several morphological characters of the hypanthium (size and form of the surface, the shape of the hypanthium) and anatomical traits (number and pattern of stomata) of apple cultivars (Malus domestica L.) with different susceptibility to fire blight were studied. The size of hypantium surface was calculated by modelling the hypanthium with a truncated cone. Three types of hypanthium surface form have been revealed: straight, convex and a complex "shouldered" one. The angle between the style and the wall of the hypanthium was narrow or wide. The stomata on the hypanthium surface can be arranged in a zone in the middle third of the hypanthium or dispersed more or less evenly. The number of stomata/flower substantially differed among the cultivars examined. The highest stomata number was detected in the flowers of the tolerant cultivar (Freedom)
No single characteristics of the hypanthium could convincingly be correlated with susceptibility to fire blight. We suggest, however, that combination of morphological properties that sustain moist environment in the hypanthium contribute to susceptibility.
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Water relations of sour cherries (minireview)
103-107.Views:230Recently, the sour cherries as food resources become more important for health preservation and so the modernization of growing technology in sour cherry production will be timely. The global warming and inadequate distribution of precipitation result a decrease in the alternancy of sour cherry production, as well. Sour cherries rather adapted to survival of drought than sweet cherry trees therefore a few studies performed to explore the water requirement of sour cherry varieties. The rootstocks, the type of soils in plantation and the water balance influence the water management of sour cherries. In orchards, in particular first year plantation, use of various row covering contribute to preservation of the natural water pool of soil and affect on the tree vigor, yield and fruit quality. Wide-spread application of integrated fruit growing technology and climate changes the researches are pointed to develop efficient irrigation technology based on transpiration yield model. The crop model based on use of meteorological data was developed for cherry orchards in order to predict transpiration of trees, dry matter production and fruit yield. The linear relationship between dry matter accumulation and transpiration was verified for sour cherry trees. Other models essay to asses the effects of climate changes on crop production. Importance of economical production and fruit quality such as ingredients of raw materials and food increases in intensive sour cherry orchards used by irrigation techniques. Because of climate changes it should more pay attention to research concerning on the stress physiological response of sour cherry varieties and post-harvest fruit quality.
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Production of transgenic carnation with a heterologous 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase bifunctional enzyme cDNA
75-79.Views:145Transgenic carnations were produced with a modified mammalian bifunctional enzyme cDNA coding 6-phosphofructo-2- kinaseffructose 2,6-bisphosphatase. Relative activity of this enzyme determines the fructose 2,6-bisphosphate (fru 2,6-P2) cytosolic concentration. This metabolite — as a signal molecule — is one of the carbohydrate metabolism regulators. The regenerated Dianthus chinensis and Dianthus caryophyllus shoots were selected on MS basal medium containing 150 mg/1 kanamycin. Transgene integration was proven by PCR analysis with cDNA specific primers followed by Southern hybridization of DNA isolated from selected green shoots, which survived on kanamycin containing medium, so 3 D. chinensis and 20 D. caryophyllus transgenic plants were produced. Transgene expression were examined by RT-PCR. Transformed and control plants were potted in glasshouse to evaluate the effect of modified fru 2,6-P2 on development, growth and carbohydrate metabolism.