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• Fire blight (Erwinia amylovora) resistance in apple varieties associated with molecular markers

  53-57.

  J. Sehic

  H. Nyboom

  L. Garkava-Gustavsson

  A. Patocchi

  M. Kellerhals

  B. Duffy

  Views:

  446

  The invasive bacterial disease fire blight, caused by Erwinia amylovora has the potential to destroy fruit tree orchards all over Europe. Effective plant protection methods are lacking in many countries, highlighting the increasing importance placed on identification of germplasm with heritable disease resistance. Recent l y. a promising QTL (quantitative trait locus) was identified on linkage group 7 in the apple cultivar 'Fiesta· which is derived from ·cox's Orange Pippin' . I n the present study, 144 Swedish and foreign apple cultivars were analysed with the SCAR markers AE I 0-375 and GE-8019. which flank-. this QTL. Twenty-nine of the analysed cultivars had both markers. 78 had either AE I 0-375 or GE-8019, and 37 cultivars did not carry an) of the two markers. Seventeen cultivars. 7 with both markers and I 0 not having either of the two markers, were then inocoluted with the bacterium in a 4uaran1i ne greenhouse test. Cultivars carrying both DNA markers were significantly less susceptible than cultivars lacking the markers, P<0.001. Cultivars that were most resistant had both markers and had 'Cox· in their pedigree. Unrelated cultivars with the markers may still lack the QTL.

• Industrial-scale plantlet production by seed priming and nursery tray seeding method in Sida hermaphrodita L. Rushby

  71-74.

  E. Kurucz

  H. R. El-Ramady

  M. G. Fári

  Views:

  402

  Virginia mallow (Sida hermephrodita Rushby), Malvaceae family, is a perspective perennial herb able to yield a biomass crop through the last two decades. In our previous studies, we examined two factors relating to seed germination percent and seed germination power during our research: the influence of hot water treatment and the effect of exogenous or endogenous infection of seed. Following these recognition we modifi ed our technique, in such a way that we fractionated the seeds based on their fresh weight / or relative density before we carried out the treatment. When we fi ltered the fl oating seeds on the surface of water, the hot water treatment was performed considerably better on the sink seeds after separation. Therefore, by this special priming process we were able to reach 80% germination capacity of Virgina mallow seeds under laboratory conditions (26 oC without illumination). From all nurseries tray methods preliminary results, it is clear that, Sida seeds, which were treated with these methods, gave the best growing rate for industrial uses.

• DNA-based determination of suitable pollinating cultivars for the pear cultivar 'Carola' (Pyrus communis)

  15-19.

  H. Nybom

  J. Sehic

  H. Mattisson

  Views:

  245

  Pollen-limited fruit set has long been suspected in some relatively low-yielding orchards with the Swedish pear cultivar 'Carola'. Fruit was therefore harvested on 23 'Carola' trees in a commercial pear orchard. The seeds were germinated and five seedlings from each tree were sampled to determine which of the surrounding cultivars had been the most successful pollinators. Leaves of 'Carola', the 7 putative pollinating cultivars and the 115 seedlings were analysed with 6 RAPD primers. By comparison of the band patterns, paternity could be ascertained for 74 seedlings. The by far most successful pollinator was 'Clara Frijs' which had sired approx. half of the seedlings, followed by 'Herzogin Elsa', `Skanskt Sockerpiiron', 'Alexandre Lucas', 'Coloree de Juillet' and 'Doyenne du Cornice'. The latter is the maternal parent of 'Carola', and these two cultivars must therefore share one S-allele and hence can only be semi-compatible. In addition, 6% of the seedlings were in all likelihood derived from selling_ since they showed no bands that did not occur also in 'Carola'. Maximum distance between 'Carola' trees and suitable pollinators should not exceed 15-20 tn. Longer distances may produce a serious dearth of compatible pol­len as evidenced by the large percentage of seedlings derived either from selling. (25%) or from long-distance (> 40 m) pollen transfer (25%) when 'Carola' trees were surrounded by non-preferred pollinators.

• Mutation induction in sweet basil (Ocimum basilicum L.) by fast neutron irradiation

  30-38.

  E. Enkhbileg

  A. Fenyvesi

  B. Bíró

  M. G. Fári

  E. Kurucz

  Views:

  703

  Basil species are highly sensitive to exterior environmental conditions and its consequences lead to great economic and agronomic losses. In this research, a mutation method was optimized out for creating a new variety of Ocimum basilicum L., which could tolerate the extreme/extraordinary climatic circumstances or biotic stresses, such as fungal diseases. Fast neutron irradiation was performed on the Hungarian commercial variety seeds with doses of 5 to 60 Gray and grown into fully developed plants. Numerous phenotypical changes like deformed congestion, leaf mutation, and low growth occurred, especially at higher dosages. Then to confirm whether the plantlets had mutation or not, and to detect the molecular variation and relationship, fingerprinting profiles of the developed mutant regenerants and donor plant have been assessed using ISSR markers. 115 loci were yielded, ranging from 0.2 to 1.5 kb, out of which 110 loci were polymorphic in nature, representing 95.6% polymorphism. The most suitable primer to determine the genetic diversity within the Ocimum species was the UBC-856 with 0.42 PIC and 4.1 MI values.

• Innovative research of ornamental plants in University of Debrecen (2001–2014)

  111-117.

  T. Kaprinyák

  Views:

  270

  In the University of Debrecen started in 2001 related and biotechnology, genetic researches on climate-changerelated.
  This work has already visible signs on the city’s public spaces. The main goal of participants of the program is it that
  in Debrecen and in the surrounding communities more adaptable to the variable climate, ecologically and biologically more
  grounded urban ornamental planting are preferred in the next decade. The new results and ongoing researches of the decorative
  ornamental and biomass plants are an interdisciplinary breeding program.

• Influence of environment in electro-chemical quality of tomato

  17-20.

  S. Kaciu

  V. Shala-Mayrhofer

  N. Mirecki

  S. Aliu

  K. Jezik

  Views:

  309

  The aim of the research was to investigate the impact of different cultivation environments of tomato cultivars, the electro-chemical and the qualitative traits of the tomato fruits. The quality of tomato fruit harvesting was evaluated at the Laboratory of Institute of Horticulture and Viticulture University of Natural Resources and Applied Life Sciences, Vienna. Two hybrids of tomatoes were tested Belle-F1 and AmaF1, in two locations and two different treatments (open field and plastic tunnels). Model of experimental fields was based on random method and included three replications. Following parameters were analyzed and tested: pH, Rh, P-Value, Nitrates and the content of vitamin C. The values of pH for treatment in the open field and indoor production system were not significant (4.47 to 5.05). For P-Value derived from the two treatments the maximal values were without significant differences, while minimal values were significant. Study has shown also that the highest values of nitrate content (16.34 mg, open field) compared to average value differences were + 0.384 mg or 43.83% higher. In indoor production the nitrate content was lower in compared with plants cultivated in open fields, so these differences were +3.81 mg or 26.4 0%, and highly significant for both levels. In relation to vitamin C content, the highest value was identified in the indoor systemof 298.6mg, with variations from the overall average of 16.43% or + 42.15 mg. The coefficient of variation values for both levels was of 29.96 respectively 27.31%.

• Prediction infection risk on the basis of weather-related factors and Erwinia amylovora colonization in apple and pear flowers

  39-54.

  T. Bubán

  E. Rutkai

  L. Dorgai

  S. V. Thomson

  Views:

  305

  Current infection risk prediction models utilize environmental parameters and field records, but do not take into account the estimated inoculum potential within the orchard. The object of this study was to survey the accuracy of three simple prediction methods under Hungarian climatic conditions, which could easily be used by the farmers. We also tested whether the accuracy of infection risk predictions can be improved by taking into consideration the incidence and/or rate of flower colonization by Erwinia amylovora.

  After preliminary investigations in 1999-2001, data concerning the weather-related infection risk were recorded in 5 apple and 1 pear orchards in 2002, and in 12 apple and I pear orchards in 2003. The weather data were processed by the easy-to-use risk assessment models of the mean temperature prediction line (MTL), Smith's Cougarblight 98C and Billing's integrated system (BIS), and by the Maryblyt^TM 4.3 computer-assisted model for reference. The population size of E. amylovora in the flower samples was estimated within an order of magnitude by PCR.

  For all years and orchards tested, Maryblyt indicated 35 days on which there was an acute infection risk. The same days were indicated by all 3 methods in 23 cases (66%), 8 days were indicated by 2 methods (23%) and 4 days were indicated by 1 method only. A similarly good correlation was found for prediction of the date of the first massive infection risk: in 2003, for instance, there was a perfectly consistent prediction by all 4 models in 9 of the 13 participating orchards. A coincidental forecast was provided by 3 of the 4 models in the other 4 orchards.

  The results indicate that any of the risk assessment models could provide an increased accuracy of the actual infection risk prediction if combined with an estimation of the incidence of Erwinia amylovora colonization in the open flowers. We found no convincing differences in the size of the epiphytic population in flowers of cultivars possessing high or low susceptibility to Erwinia amylovora.

  We conclude that the easy-to-use methods tested could be used by the fanners to recognize weather-related risks, especially when coupled with an estimation of the proportion of the pathogen-infested flowers. This local prediction would provide rapid information (faster than the regional forecast systems) specifically for a given orchard.

• Yielding capacity and regeneration of different ages fruit-bearing shoots in sour cherry

  55-57.

  I. Gonda

  Z. Such

  Views:

  428

  The yielding potential and regeneration (i.e. germination of hidden buds) after pruning of the fruit-bearing shoots of different ages were compared in sour cherry cultivars pruned to spindle shape. The examinations were carried out in order to determine the optimal pruning strategy resulting in the highest long-term yielding of sour cherry cultivars with intensive crown shape (free and thin spindle shape). According to our results, there are great differences among the studied self-fertile sour cherry cultivars in yielding and regeneration, which are worthwhile to be considered in determining the method and degree of pruning.

• Conventional PCR primers for the detection of grapevine pathogens disseminated by propagating material

  69-80.

  E. Manduláné Farkas

  N. Czotter

  R. Lózsa

  T. Dula

  I. Ember

  É. Várallyay

  E. Szegedi

  Views:

  490

  Polymerase chain reaction driven by sequence specific primers has become the most widely used diagnostic method to detect and identify plant pathogens. The sensitive and cost-effective pathogen detection is exceptionally important in the production of propagating material. In this paper we have collected primer sequence data from the literature for the detection of the most important grapevine pathogens disseminated by propagating stocks by conventional polymerase chain reaction. Basic protocols to obtain template nucleic acids have also been briefly rewieved.

• A review of the orchard management in organic fruit production

  43-49.

  I. Gonda

  I. J. Holb

  Views:

  617

  The elements of orchard management have a determining role in the success of organic fruit production, therefore, it is essential that they are applied properly. The present review gives describes some aspects of the orchard management such as site selection, orchard soil, rootstocks and cultivars, planting materials and plant density, crown shape. Then, one of the most important elements, pruning is discussed with special emphasis on winter pruning, condition and apical dominance, degree and method of pruning, summer pruning. After pruning, an overview of fruit thinning, irrigation, soil cultivation, soil protection (cover plants and mulching) and fertilization is given.

• Comparative study of sour cherry samples for their anthocyanin content measured by tools of the laboratory and by portable equipments

  29-31.

  G. Szemán-Nagy

  G. Pintér

  J. Prokisch

  J. Tornyai

  J. Nyéki

  Z. Szabó

  T. Szabó

  M. G. Fári

  Views:

  924

  The inner quality of fruits depends on many components. The analysis of pharmacologically important fruit species as the sour cherry consumed as a common food is an expensive task, mostly requiring an equipped laboratory. In sour cherry, one of its valuable components, the anthocyanin content deserves special attention. A portable analytical implement, which has been developed by our team measures the anthocyanin content on a scale of 1024 PharMgrades. The system is a member of the UVEX family of implements working with a microprocessor and performs the measurement quickly. The portable variant (UVEX-ML-1) does not require much training, the laboratory variant (UVEX UL-1) performs a large quantity of tests under modest laboratory conditions. Preparation of the samples is easily done, the necessary elements are available in the trade. The reagent is easily stored and dosed. The system was tested and proved to be of sufficient precision and the result showed acceptable variance according to the checks performed by a spectrophotometer in the laboratory.

• Primers designed for the detection of grapevine pathogens spreading with propagating material by quantitative real-time PCR

  21-30.

  N. Czotter

  E. Manduláné Farkas

  R. Lózsa

  I. Ember

  G. Szûcsné Varga

  É. Várallyay

  E. Szegedi

  Views:

  480

  Several grapevine pathogens are disseminated by propagating material as systemic, but latent infections. Their detection and identification have a basic importance in the production and handling of propagating stocks. Thus several sensitive and reliable diagnostic protocols mostly based on molecular techniques have been developed. Of these methods quantitative real-time PCR (q-PCR) has recently got an emerging importance. Here we collected primer data for the detection and identification of grapevine pathogens which are important in the production of propagating stocks by q-PCR. Additional novel techniques that use DNA amplification, hybridization and  sequencing are also briefly reviewed.

• Agrobacterium transformation of Rhodiola sp.: current status and limitations

  37-42.

  A. Javid

  Zs. György

  Views:

  330

  The study of secondary metabolites has led to the discovery of new drugs for treating human diseases. However, consistent plant supply can be challenging, leading to the use of plant tissue culture techniques such as hairy root culture. Hairy roots have stable genetics, lateral branching, and can produce secondary metabolites, including alkaloids, flavonoids, and terpenoids. Research on hairy roots as a subject began in the late 19^th century, and for the last four decades, hairy roots have been utilized for producing secondary metabolites and recombinant proteins. This article focuses on Rhodiola species - genus of perennial plants that belongs to the family Crassulaceae - and its potential as a source of secondary metabolites using hairy root culture techniques. Rhodiola sp. is widely distributed throughout the Arctic regions of the Northern Hemisphere, with several species having significant medicinal properties. The article discusses the possible use of hairy root cultures for the production of Rhodiola secondary metabolites, including salidroside and rosavins, which have demonstrated significant pharmacological activity in various studies. The use of elicitation and genetic engineering techniques to boost secondary metabolite production in Rhodiola hairy roots is also explored. Overall, the article highlights the potential of Rhodiola hairy root cultures as a valuable source of secondary metabolites with medicinal properties. However, despite some studies Rhodiola hairy root induction and culturing still remains highly unexplored.

• Field evaluations of 14 sweet cherry cultivars as pollinators for cv. Regina (Prunus avium, L.)

  75-77.

  S. Thurzó

  M. Grandi

  L. Lagezza

  S. Lugli

  I. J. Holb

  T. Szentpéteri

  P. T. Nagy

  Z. Szabó

  M. Soltész

  J. Nyéki

  S. Sansavini

  Views:

  1151

  In this study, the pollen of 14 sweet cherry cultivars (‘Anella’, ‘Duroni 3', 'Badacsony', 'Cristalina', 'Ferbolus', 'Ferrovia',

  'Georgia', 'Hudson', 'Kordia', 'Sam', 'Schneiders’, ‘Spate’, ‘Knorpelkirsche', 'Skeena', 'Summit', 'Sylvia') was used to fertilize the emasculated flowers of sweet cherry cv. 'Regina'. Fruit set was assessed three times during fruit development: 14 May, 30 May and 27 May 2007. We observed full incompatibility among the 14 cultivars for cv. 'Cristalina', which is in the same S-allele group as cv. 'Regina'. After analysis of our data, we have results about fertilization efficiency of the cultivars. Most of the evaluated cultivars are inadequate to fertilize cv. 'Regina' to a sufficient degree. There were two exceptions, cv. 'Sam' and cv. 'Skeena', where percentage of ripened fruits was above 20%. These two cultivars can guarantee such a pollination, which ensures ample quantity of ripened fruits. Results of this study have proved three other cultivars to be quite good pollinators for cv. 'Regina'. In conclusion, ideal pollinators for cv. 'Regina' could be — apart from above-mentioned two cultivars, 'Sam' and 'Skeena' — cvs. 'Sylvia' and 'Bianca', which was suggested by more literature sources.

• Effects of methyl jasmonate, salicylic acid and phenylalanine on aloe emodin and aloin in diploid and tetraploid Aloe barbadensis

  S. Shafighi

  A. Moieni

  S. Rashdi Monfared

  Views:

  438

  Aloe vera is one of the most famous medicinal plants. Aloin and aloe emodin are the most important active compounds in this plant. The purpose of this research was the comparison of aloin and aloe emodin production after the elicitation by methyl jasmonate, salicylic acid, and phenylalanine in diploid and tetraploid Aloe vera plants in greenhouse conditions. The plants were treated with the concentrations of 25, 50, and 100 µM. The amounts of aloin and aloe emodin were determined 24 and 48 hours after application of the treatment. HPLC analysis showed that the leaves of the control diploid plants (without applying elicitors) had more aloin (1.20 fold) and aloe emodin (1.14 fold) than the control tetraploid plants. The maximum concentration of aloin (1.15 ± 0.07 µg mg-1 dry weight) was obtained after the elicitation by 25 µM methyl jasmonate, 24 hours after treatment, in diploid plants) 6.36 fold compared to the control (0.18 µg mg-1 dry weight (. In addition, the maximum concentration of aloe emodin (0.28 µg mg-1 dry weight) was obtained after the elicitation by 25 µM salicylic acid, 24 hours after treatment, in diploid plants) 6.18 fold compared to the control (0.04 µg mg-1 dry weight)). The long-term effect of three studied elicitors (after 240 days) on plant health and survival was also studied. This investigation showed that only methyl jasmonate at a concentration of 100 µM was resulted in the death of Aloe vera plants.

• Genetic diversity in a collection of apple (Malus x domestica Borkh.) cultivars as revealed by RAPD markers

  25-35.

  L. Garkava-Gustavsson

  H. Nybom

  Views:

  334

  A collection of 151 apple cultivars was investigated with 7 RAPD primers generating 71 informative bands, to evaluate genetic variability and relatedness. All cultivars presumably derived through genetic recombination were distinguished whereas identical DNA profiles indicated that some cultivars had arisen as sports. A cluster analysis and a PCO did not reveal any distinct geographic patterns, but there was a weak tendency for Swedish and foreign cultivars to differentiate.. Many cultivars however clustered together with either one of their parents or with siblings. Overall genetic diversity among the 151 cultivars was estimated with Nei's diversity index (H), 0.269, and with Shannon's index (H'), 0.594. The cultivars were also analysed in six groups, according to time of origination and country of origin, with an average H = 0.262 and H' = 0.546. No major differences in genetic diversity were observed over time or space, although the group with recent, foreign cultivars had the lowest diversity (FL = 0.235, H' = 0.493). Comparison between the entire material and a subset with 94 mandate cultivars chosen for preservation in Sweden, showed similar genetic diversity: HFN.rj1E = 0.268, fr_E,_NTIRE, = 0.593 and HMANDATE = 0.263, WMANDATE = 0.575. No major differences in band frequencies were observed between these two sets, but 5 RAPD bands were missing in the set with mandate cultivars.

• Genetic engineering of apple (Malus domestica Borkh.) for resistance to fungal diseases using g2ps1 gene from Gerbera hybrida (Asteraceae)

  15-12.

  N. M. Ali Bacha

  M. Batha

  A. M. Abdul-Kader

  Views:

  420

  In the present study, g2ps1 gene from Gerbera hybrida coding for 2-pyrone synthase which contribute for fungal and insect resistance was used. The aim was to work out an efficient approach of genetic transformation for apple cvs. ‘Golden Delicious’, ‘Royal Gala’ and ‘MM111’, ‘M26’ rootstocks for improving their fungal resistance using genetic engineering techniques. Adventitious shoot formation from leaf pieces of apples studied was achieved using middle leaf segments taken from the youngest leaves from in vitro-grown plants.
  Optimum conditions for ‚direct’ shoot organogenesis resulted in high regeneration efficiency of  0%, 95%, 92%, 94% in the studied apples respectively. Putative transgenic shoots could be obtained on MS media with B5 Vitamins, 5.0 mg l-1 BAP, or 2.0 mg l-1 TDZ with 0.2 mg l-1 NAA in the presence of the selection agent “PPT” at 3.0-5.0 mgl-1. Shoot multiplication of transgenic shoots was achieved on: MS + B5 vitamins + 1.0 mg l-1 BAP + 0.3 mg l-1 IBA, 0.2 mg l-1 GA3+1.0 g/l MES+ 30 g/l sucrose + 7.0 g/l Agar, with the selection agent PPT at 5.0 mg l-1 and were subcultured every 4 weeks in order to get sufficient material to confirm transformation of the putative shoots obtained. Six, seven, one and six transgenic clones of the apples studied respectively have been obtained and confirmed by selection on the media containing the selection agent “PPT” and by PCR analysis using the suitable primers in all clones obtained for the presence of the selection” bar gene (447 bp) and the gene-of- interest “g2PS1” (1244 bp), with transformation efficiency of 0.4%, 0.6%, 0.1% and 0.3% respectively. These transgenic clones were multiplied further in vitro in the presence of the selection agent ‘PPT’ and rooted in vitro. Rooted transgenic plantlets were successfully acclimatized and are being kept under-containment conditions according to the biosafety by-law in Syria to evaluate their performance for fungal resistance .

• Irrigation Requirements for Grape Crop under Climate Changes Conditions in Egypt

  23-27.

  F. A. Hashem

  M. K. Hassanein

  A. A. Khalil

  É. Domokos-Szabolcsy

  M. Fári

  Views:

  466

  The present work is mainly directed to discuss sensitivity of climate changes upon the irrigation demand for grape crop in Egypt. The Penman Monteith equation was used to calculate reference Evapotranspiration (ETo) under current and future climate for the two locations (El Menya and El Beheira). The historical climate data for ten years from (2000 – 2010) was used as current climate to calculate irrigation requirement for grape crop under Egyptian conditions. Two climate changes scenarios have been applied as changes in temperature. The first scenario supposed that increasing in temperature of 1.5°C would happen, and the second scenario supposed that increasing of 3.5°C would happen to calculate reference Evapotranspiration and irrigation requirement for future climate. The results showed that the evapotranspiration and irrigation requirement for grape crop at El Menya location higher than El Beheira location. Irrigation demand for grape plant under two climate changes scenario will increase in El Menya and El Beheira locations. El Menya location will take the highest irrigation demand under climate changes. Therefore, possible adaptation countermeasures should be developed to mitigate the negative effects of climate changes for the sustainable development of agro-ecosystems in Egypt.

• Total anthocyanine content and antioxidant density of some Hungarian sour cherry varieties

  109-111.

  Zs. Veres

  I. J. Holb

  J. Nyéki

  Z. Szabó

  M. G. Fári

  Views:

  1346

  Evidence suggests that a diet high in fruits and vegetables may decrease the risk of chronic diseases, such as cardiovascular disease and cancer, and phytochemicals including phenolics, flavonoids and carotenoids from fruits and vegetables may play a key role in reducing chronic disease risk. Recent research has proven that sour cherry (Prunus cerasus L.) is a valuable natural source of some bioactive compounds important in human health preservation. In our work, we identified the total antioxidant activity, anthocyanine content and "antioxidant density" of sour cherry varieties named "Újfehértói fürtös", "Debreceni bőtermő", "Kántorjánosi" and "Érdi bőtermő" and those of the "Bosnyák" sour cherry clones. "Antioxidant density" is a biological value indicator obtained in a synthetic way, which indicates the antioxidant capacity of the particular food, e. g. fruit and vegetable, related to 1 Calorie.

• Luminescence variations in cucumber (Cucumis sativus L.) leaves derived from different regeneration systems

  50-52.

  A. Murkowski

  T. Wróblewski

  W. Burza

  E. Skórska

  Views:

  271

  Plants obtained from in vitro culture can show increased susceptibility to environmental stress conditions. In the process of their adaptation to natural conditions it requires monitoring of their physiological state. The methods used to check this phenomenon should estimate quickly and exactly the tolerance to suboptimal environmental factors. Such requirements are satisfied by the methods of measuring chlorophyll luminescence in vivo, e.g. fluorescence induction and delayed luminescence. The objects of our studies were cucumber plants regenerated from cultures of callus and embryogenic cell suspension, as well as the plants obtained from seeds. The plants derived from in vitro cultures displayed a poor physiological condition at the early phase of adaptation characterised by higher susceptibility both to stress caused by increased density of the light flux and low temperature (4 °C) in comparison with the plants obtained from seeds.

   

• Study on the viruses of Petunia in Hungary

  55-59.

  É. Baracsi

  E. K. Tóth

  E. Kriston

  A. Takács

  D. Pribek

  J. Horváth

  Views:

  262

  Virus susceptibility of 11 varieties of vegetatively propagated petunia against 2 virus strains (TMV-C/U1 and PVYNTN) were examined. Neither resistant nor tolerant varieties of petunia were found. The virus infection of the Petunia genus was examined in Hungary. The most common pathogens were the Tobacco mosaic tobamovirus (TMV) and the Tomato mosaic tobamovirus (ToMV), but the samples also contained Alfalfa mosaic alfamovirus (AMV), Cucumber mosaic ClIC11171014171S (CMV) and Potato Y potyvirus (PVY). Potato X potexvirus (PVX) was isolated in the varieties of trailing petunia for the first time.

• Effect of three storage methods on fruit decay and brown rot of apple

  55-57.

  B. Balla

  I. J. Holb

  Views:

  1025

  The aim of our two-year study was to evaluate fruit decay and Monilinia fruit rot in three controlled atmospheres (CA), ultra-low oxygen (ULO) and traditional storage methods on apples for a duration of several months storage period. Four phytopathological treatments were studied under each storage condition: 1) 48 healthy fruit per unit, 2) 48 injured fruit per unit, 3) 47 healthy fruit and 1 brown rotted fruit per unit, and 4) 47 injured fruit and 1 brown rotted fruit per unit. Our results clearly demonstrated that fruit loss during storage is highly influenced by storage conditions and health status of the stored fruits. In the 2005 experiment, the lowest and largest fruit decay occurred under the ULO and traditional storage conditions, respectively (Table 1). The fruit decay was significantly different for the different storage methods. Fruit decay was fully suppressed in ULO storage except in the treatments of injured and injured + 1 brown rotted apple. Under CA and traditional storage conditions, when healthy fruit was stored, fruit decay was significantly lower compared with injured fruit including 1 brown rotted fruits. However, half of the fruit decay was caused by M. fructigena in CA store irrespective to phytopathogenic treatments. In 2006, results were not so consistent on cv. Idared but were not essentially different from the 2005 experiments.

• Optimizing disinfection protocols for yam explant regeneration in plant tissue culture

  66-72.

  Omotayo Komolafe

  Oyindamola H. Oyinloye

  Ejeoghene R. Ogbimi

  Kasali. O. Hassan

  Olugboyega S. Pelemo

  Views:

  650

  Yam (Dioscorea species), does not produce commercially viable seeds, and asexual propagation is faced with challenges resulting from carried-over infections from previous generations. Contamination is a prevalent problem in Plant Tissue Culture (PTC), making the development of cost-effective and efficient disinfection protocols crucial for successful PTC. This study aimed to evaluate the effectiveness of yam explant disinfection protocols using various immersion timings, disinfectant concentrations (including ethanol (C₂H₅OH), sodium hypochlorite (NaOCl), and hydrogen peroxide (H₂O₂)), and in single or combined disinfectants application. Twenty treatment combinations and one control were assessed on yam vines for Disinfection Efficiency (DE%), Negative Disinfection Effect (NDE%), and the regeneration of shoots and roots (SN & RN) from the culture after 21 days. The study showed that varying immersion times did not significantly impact the evaluated parameters. However, different concentrations of disinfectants resulted in diverse NDE responses. Surprisingly, higher concentrations of NaOCl led to reduced NDE, whereas lower concentrations increased NDE. On the contrary, higher concentrations of H₂O₂ increased NDE, while lower concentrations decreased it. Shoot and root regeneration rates were also significantly impacted by the choice of disinfection protocol. The research concluded that dual disinfection protocol, specifically 70% ethanol for 7 minutes followed by NaOCl, was most effective for eliminating surface-borne contaminants and achieving successful in vitro propagation of yam plantlets. This method offers a cost-effective solution for establishing microbe-free tissue culture yam plantlets and provides a basis for future research on other Dioscorea plants.

• Study of genetic transformation efficiency via organogenesis and embryogenesis in eggplant (Solanum melongena L. cv. Embti): effects of co-culture, temperature and kanamycin and hygromycin-based selection procedures

  15-23.

  E. A. de T. Picoli

  S. H. Brommonschenkel

  P. R. Cecon

  D. J. H. Da Silva

  M. G. Fári

  W. C. Otoni

  Views:

  408

  The effects of kanamycin and hygromycin-based selection and co-culture temperature ranging from 22 to 28 °C upon eggplant transformation efficiency were evaluated. Both morphogenic pathways, somatic embryogenesis and organogenesis, were adopted using cotiledonary and hypocotyl explants, respectively. Somatic embryos were recovered in the presence of both antibiotics, although lesser escapes were observed in hygromycin-supplemented medium. Indeed, selection provided by this antibiotic was more efficient compared to kanamycin, nevertheless, shoot regeneration was not observed with hygromycin. Significant difference on the frequency of cotiledonary explants displaying callus (FEC) was observed as embryogenesis was concerned, although a higher number of embryos was observed in hygromycin selective media. The frequency of explants presenting callus (FEC), embryos (FEE) and shoots or buds (FERG) did not differ statistically for the tested co-culture temperatures, although higher regenerant number was observed at 24 °C.

• Results on mating disruption by sex pheromones against moth pests of apple in integrated and organic orchards

  51-53.

  I. Újvári

  I. J. Holb

  Views:

  679

  The study was aimed to study that how mating disruption by hand applied dispensers can reduce the number of damage caused by Cydia pomonella, Adoxophyes orana and Pandemis heparana in four integrated and organic apple orchards. In the first orchard (Gacsály), protection against moth caterpillars ensured by IPM and conventional production systems were equally good, but worse than that of the orchard part where mating disruption was applied by 1000 dispensers/ha. In second orchard (Nyírbogdány), the highest incidence of codling moth damage was measured in the hilly part (17%), while in the plot where 440 dispensers/ha pheromone dosage was applied, the damage incidence was 11%. The smallest damage incidence was at the flat part, where 666 dispensers /ha was applied. In the third and fourth orchards (Eperjeske), codling moth damage on fruits was below 7% in the larger and smaller orchards where 1000 dispensers/ha was applied. At Eperjeske, the codling moth damage increased by 32.3% in the field treated with Bacillus thuringiensis product but without using mating disruption. The results verified that the use of 1000 dispensers/ha as suggested by the manufactures is essential, especially in the first year of application. The results also suggested that better results can be achieved in flat areas and the larger plot size also enables a more efficient reduction of the damage.