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International Journal of Horticultural Science

Vol. 31 (2025)
Articles

Optimizing disinfection protocols for yam explant regeneration in plant tissue culture

Published:
2025-07-08
DOI Linkhttps://doi.org/10.31421/ijhs/31/2025/15325
Authors
Omotayo Komolafe,
Oyindamola H. Oyinloye,
Ejeoghene R. Ogbimi,
Kasali. O. Hassan,
Olugboyega S. Pelemo
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Keywords
Dioscorea rotundata disinfection protocol contamination control disinfection efficiency regeneration
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Copyright (c) 2025 International Journal of Horticultural Science

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This work is licensed under a Creative Commons Attribution 4.0 International License.

This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

How To Cite
Selected Style: APA
Komolafe, O., Oyinloye, O. H., Ogbimi, E. R., Hassan, K. O., & Pelemo, O. S. (2025). Optimizing disinfection protocols for yam explant regeneration in plant tissue culture. International Journal of Horticultural Science, 31(1), 66-72. https://doi.org/10.31421/ijhs/31/2025/15325
Abstract

Yam (Dioscorea species), does not produce commercially viable seeds, and asexual propagation is faced with challenges resulting from carried-over infections from previous generations. Contamination is a prevalent problem in Plant Tissue Culture (PTC), making the development of cost-effective and efficient disinfection protocols crucial for successful PTC. This study aimed to evaluate the effectiveness of yam explant disinfection protocols using various immersion timings, disinfectant concentrations (including ethanol (C2H5OH), sodium hypochlorite (NaOCl), and hydrogen peroxide (H2O2)), and in single or combined disinfectants application. Twenty treatment combinations and one control were assessed on yam vines for Disinfection Efficiency (DE%), Negative Disinfection Effect (NDE%), and the regeneration of shoots and roots (SN & RN) from the culture after 21 days. The study showed that varying immersion times did not significantly impact the evaluated parameters. However, different concentrations of disinfectants resulted in diverse NDE responses. Surprisingly, higher concentrations of NaOCl led to reduced NDE, whereas lower concentrations increased NDE. On the contrary, higher concentrations of H2O2 increased NDE, while lower concentrations decreased it. Shoot and root regeneration rates were also significantly impacted by the choice of disinfection protocol. The research concluded that dual disinfection protocol, specifically 70% ethanol for 7 minutes followed by NaOCl, was most effective for eliminating surface-borne contaminants and achieving successful in vitro propagation of yam plantlets. This method offers a cost-effective solution for establishing microbe-free tissue culture yam plantlets and provides a basis for future research on other Dioscorea plants.

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