Vol. 8 No. 3-4 (2002)
Articles

Study of genetic transformation efficiency via organogenesis and embryogenesis in eggplant (Solanum melongena L. cv. Embti): effects of co-culture, temperature and kanamycin and hygromycin-based selection procedures

Published October 16, 2002
E. A. de T. Picoli
Departamento de Biologia Vegetal and Institute de Biotecnologia Aplicada a Agropecuaria BIOAGRO
S. H. Brommonschenkel
Departamento de Biologia Vegetal and Institute de Biotecnologia Aplicada a Agropecuaria BIOAGRO; Departamento de Fitopatologia
P. R. Cecon
Departamento de Biologia Vegetal and Institute de Biotecnologia Aplicada a Agropecuaria BIOAGRO; Departamento de Informatica
D. J. H. Da Silva
Departamento de Fitotecnia. Universidade Federal de Vicosa, 36571-000, Vigosa, MG, Brasil.
M. G. Fári
Department of Vegetable Crops & Horticultural Biotechnology, University of Debrecen, Centre of Agricultural Sciences, H-4032 Debrecen, Böszörményi út 138, Hungary
W. C. Otoni
Departamento de Biologia Vegetal and Institute de Biotecnologia Aplicada a Agropecuaria BIOAGRO
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APA

Picoli, E. A. de T., Brommonschenkel, S. H., Cecon, P. R., Da Silva, D. J. H., Fári, M. G., & Otoni, W. C. (2002). Study of genetic transformation efficiency via organogenesis and embryogenesis in eggplant (Solanum melongena L. cv. Embti): effects of co-culture, temperature and kanamycin and hygromycin-based selection procedures. International Journal of Horticultural Science, 8(3-4), 15-23. https://doi.org/10.31421/IJHS/8/3-4/355

The effects of kanamycin and hygromycin-based selection and co-culture temperature ranging from 22 to 28 °C upon eggplant transformation efficiency were evaluated. Both morphogenic pathways, somatic embryogenesis and organogenesis, were adopted using cotiledonary and hypocotyl explants, respectively. Somatic embryos were recovered in the presence of both antibiotics, although lesser escapes were observed in hygromycin-supplemented medium. Indeed, selection provided by this antibiotic was more efficient compared to kanamycin, nevertheless, shoot regeneration was not observed with hygromycin. Significant difference on the frequency of cotiledonary explants displaying callus (FEC) was observed as embryogenesis was concerned, although a higher number of embryos was observed in hygromycin selective media. The frequency of explants presenting callus (FEC), embryos (FEE) and shoots or buds (FERG) did not differ statistically for the tested co-culture temperatures, although higher regenerant number was observed at 24 °C.

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