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Testing the virulence of some Hungarian Erwinia amylovora strains on in vitro cultured apple rootstocks
52-55.Views:167A useful method was improved to test and to evaluate the susceptibility of plants to fire blight and the virulence of E. amylovora strains. Six Hungarian strains from different host plants were tested on in vitro cultured apple rootstocks. Disease rating was used for the characterization of the process of disease development. The different strains had different capacity to cause disease, mainly in the first period of incubation. There were significant differences between the virulence of the strains.
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Experimental approach in apple tree nutrition
9-13.Views:123Authors present synthesis of experimental work, performed in the last decades, for better understanding nutritional behaviour of apple trees and related problems in fruit quality. There were evidences supporting possible deteriorating role of potassium in feeble physiological status of apples, if applied in excess. More intensive studies proved that higher potassium uptake into leaves and fruits might be also the result of increased sink power of individual fruits. Nevertheless early senescence of apples during storage and also sensibility to bitter pit were successfully related to the increased sink power of fruits, casual relations in excessive NPK fertilization, although increase in sink power need further investigations. Impaired weather conditions during early development of fruits, hostile orchard practices in pruning, thinning, irrigation and also unskilled application of growth regulators may also contribute in the enhancement of sink power and in weakened physiological status of apple fruits.
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Evaluation of generative accomplishment of new apple cultivars in Hungary
11-15Views:254In a two year study, 14 new apple cultivars (cvs ’Gala Venus Fengal’, ’Gala Decarli-Fendeca’, ’Galaval’, ’Jugala’, ’Gala Schnitzer (S) Schniga’, ’Red Cap Valtod (S)’, ’Early Red One’, ’Jeromine’, ’Crimson Crisp (Co-op 39)’, ’Red Topaz’, ’Wilton’s Red Jonaprince’, ’Red Idared’, ’Fuji September Wonder’ and club cultivar) were studied in Eastern Hungary at Nyírbátor. The following parameters were measured: trunk cross sectional area (cm2), harvest time with yield (date, kg/tree, number/tree), fruit size (mm), fruit surface color (%), and color intensity of the fruit surface (1-5). According to our two year assessments regarding, the best generative accomplishment was achieved for the cvs ‘Gala’ sorts trained to slender spindle and the cv ‘Red Jonaprince’ trained to super spindle. According to the trunk cross sectional area the ‘Gala’ sport trained to slender spindle showed the strongest growth among the 4 years old trees, while the cv ‘Fuji SW’ and the cv ‘Jeromine’ presented lower vegetative accomplishment. The ripening time of the cultivars started at the end of August and finished at the beginning of October. ‘Gala’ mutants trained to slender spindle provided the highest yields. Fruit size of all cultivars reached the market required standard, however, cvs ‘Red Jonaprince’ and ‘Red Idared’ produced large fruits over 90 mm. The fruit size of the ‘Gala’ sort was 72–75 mm. Most of the cultivars showed high fruit surface color and color intensity.
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Mechanical and physical control in apple orchards as preventative fungal disease management
19-21.Views:249In this minreview, mechanical and physical control against apple fungal diseases among non-chemical control approaches were summarized. This overview listed five groups of mechanical and physical control methods: pruning, removal of inoculum sources, shredding of leaf litter, burying of inoculum sources and flaming of leaf litter. These methods were shown to reduce succesfully infection potential of inoculum sources in orchards and these non-chemical control measures are one of the most essential approaches for preventative fungal disease management. However, most of these methods are not widely spread in the apple-growing practice due to relatively low control efficacy, medium to high labour costs and/or time limits during the season.
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Monitoring of water regime in an apple orchard
29-32.Views:229Our investigation was carried out at an micro-irrigated intensive apple orchard in Debrecen-Pallag in 2010. The aims of the study were to monitor the effect of a compacted layer on soil water regime by tensiometers and supporting the water management of the orchard. The results suggest that the physical characteristic of the examined soil is sandy soil with low capillarity and total available water content. The soil water tensions were varied between pF 0 and 2.5 due to the extreme precipitation circumstances in 2010. Tensiometers in 40 cm depth resulted fast (few hours) and significant respond to precipitation than in the 70 cm soil layer. Based on daily measurements, the soils possess a daily fluctuation of soil moisture, however the changes become more moderate in deeper layers. In accordance with all of the results, the amount of drainable water regime was about 20.6 V/V% at 40 cm depth and 18.6 V/V% at 70 cm mainly. The harmful surplus water can be infiltrated by loosening of the compacted soil layer in 50–70 cm depth or led off by vertical drainage.
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Study of Erwinia amylovora colonization and migration on blossoms of susceptible and tolerant apple cultivars
15-19.Views:132The stigmata of detached flowers of susceptible and tolerant apple cultivars were inoculated with about 104 gfp labeled Erwinia amylovora . There were no apparent differences in the colonization, multiplication and survival of the bacteria on the stigmatic surface of the culivars. Bacteria were washed down to the hypanthium surface 24 hours after inoculation. The visual symptoms of the infection were the discoloration and shrinkage of the floral parts. The gradual browning associated with the infection appeared first on the surface of the hypanthium followed by the discoloration of the style. The color of the filaments turned into brown only 120 hours after the inoculation. Bacterial cells were not detected in the tissues of the styles and filaments. The traits of the hypanthium surface are of prominent importance in the progression of the infection. The wrinkled surface, the convex shape of the outer epidermal cell walls with thin cuticle and the sunken stomata helped to preserve a water film for a longer period providing medium for the motility of the bacteria in the susceptible cultivar. Bacteria were restricted to small water droplets on the flat and waxy surface of the hypanthium of the tolerant cultivar and only a few were able to enter the tissues.
Large bacterium aggregations were detected in the intercellular spaces of the parenchyma of the susceptible cultivar 48 hours after the inoculation. In the next period the Erwinia amylovora cells gradually invaded the intercellulars of the hypanthium wall, the wall of the ovary and the pedicel. Low level of bacterium aggregation was found in the intercellulars of the tolerant cultivars. It is suggested that the progression of the infection was inhibited also by physiological factors.
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Effect of postharvest sodium benzoate treatment on some fruit parameters of two organic apple cultivars
35-37.Views:389The aim of this study was to investigate the effect of postharvest sodium-benzoate treatment on some fruit parameters of two organic apple cultivars (’Topaz’ and ’Florina’). Assessments were made at three times during storage: 17 November 2019, 20 December 2019 and 23 January, 2020. During every assessment dates, each fruit was observed separately, and determined the proportion of i) healthy fruits (%), ii) post-harvest fruit rot diseases iii) fruits with mechanical injury (%), iv) fruit with russetting (%), v) damage of codling moth and vi) Ca-deficiency symptoms. In addition, fruit weight (g) was measured at each assessment date. Sodium benzoate reduced the fruit decay and the proportion of healthy fruit was higher in this treatment compared to water treated fruit. This effect could be seen in all assessment dates and on both cultivars. Effects on other parameters were various according to cultivar and assessment dates. Loss of fruit weight was similar in both treatments and cultivars compared to control treatment.
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Effect of three storage methods on fruit decay and brown rot of apple
55-57.Views:534The aim of our two-year study was to evaluate fruit decay and Monilinia fruit rot in three controlled atmospheres (CA), ultra-low oxygen (ULO) and traditional storage methods on apples for a duration of several months storage period. Four phytopathological treatments were studied under each storage condition: 1) 48 healthy fruit per unit, 2) 48 injured fruit per unit, 3) 47 healthy fruit and 1 brown rotted fruit per unit, and 4) 47 injured fruit and 1 brown rotted fruit per unit. Our results clearly demonstrated that fruit loss during storage is highly influenced by storage conditions and health status of the stored fruits. In the 2005 experiment, the lowest and largest fruit decay occurred under the ULO and traditional storage conditions, respectively (Table 1). The fruit decay was significantly different for the different storage methods. Fruit decay was fully suppressed in ULO storage except in the treatments of injured and injured + 1 brown rotted apple. Under CA and traditional storage conditions, when healthy fruit was stored, fruit decay was significantly lower compared with injured fruit including 1 brown rotted fruits. However, half of the fruit decay was caused by M. fructigena in CA store irrespective to phytopathogenic treatments. In 2006, results were not so consistent on cv. Idared but were not essentially different from the 2005 experiments.
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Growing characteristics of apple cultivars and canopies
7-10.Views:284In our study we aimed to evaluate the growing characteristics of new prospective apple cultivars and their canopies. The following parameters were measured: I) thickness of the trunk and the central leader, II) ratio of the trunk thickness and the basic branches (Zahn indexes), III) number of the branches of the central leader, and iv) thickness of the branches of the central leader. The main branches of the trunk and the central leader tend to over thickening in the case of cvs ‘Red Idared’, ‘Wilton’s Red Jonaprince’ and ‘Jeromine’. The density of the branches of certain parts of the canopy was too low for cvs ‘Crimson Crisp’, ‘Wilton’s Red Jonaprince’, ‘Fuji September Wonder’ and ‘Red Idared’. The density of the branches of certain parts of the canopy was too high for cvs ‘Gala Venus Fengal’, ‘Wilton’s Red Jonaprince’ and ‘Red Cap Valtod’. Relationship was found between the tapering dynamics of the central axis and the thickness of the main branches, which was stronger in super spindle canopies than in slender spindle crown forms.
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Rootstock effects on fruit drop and quality of 'Arlet' apples
69-75.Views:199The aim of this study is to investigate the effect of different growth inducing rootstocks on fruit drop of apple. This research was conducted for 3 years at Nagykutas, Western Hungary on apple cv. 'Arlet'. For the experiments, 3 different growth inducing rootstocks were choosen: M.9 (weak), MM.106 (moderate) and crabapple seedling (strong). There were 3 fruit shedding periods on the trees grafted M.9 and MM.106 rootstocks, but in the case of crabapple seedling, only 2 were found. The measure of fruit drop was closely related to seed count of fruits; seed number was the lowest, fruit drop was the highest. The lowest seed number was counted in fruits from trees on crabapple seedling. Seasonal changes of leaf:fruit ratio mainly depended on shoot growth and fruit drop. The rise of the curve of leaf:fruit ratio was very important during the first phase of fruit development, in especially at the end of June and in the beginning of July. The cause of this is that first and second periods of fruit drop appeared during this term. The rise of the curve was important in the beginning of June and the end of July on crabapple seedling. Decreasing tendency of quality parameters was found of fruits from trees on M.9, MM.106 and seedling rootstocks, except flesh firmness which was the highest in fruits from trees on crabapple seedling.
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The expression of the primigenic dominance in the flowering and fruit set of selected apple cultivars on different growth inducing rootstocks
27-31.Views:200Authors investigated the expression of the primigenic dominance in the flowering and fruit from open and self pollination of four apple cultivars ('Gala Royal', 'Golden Smoothee', Pink Lady' and 'Vista Bella') during two consecutive years in Western Hungary on three different growth inducing rootstocks (M. 9, MM. 106 and seedling). There were not significant differences in the effect of the rootstocks on the flowering order in a flower cluster. Significant difference in the fruit set in open pollination was found among individual flowers in a cluster, mostly between the king bloom and the second flower. The rate of the fruit set from self-pollination was very low without any significant difference among individual flowers in the cluster.
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Comparative organoleptic examination of apple varieties developed by different environmentally safe technologies
69-70.Views:144Ecological or biological growing techniques are generally recognised as actual trends in agricultural production. The objective of our experiments is a comparison of apple fruits grown alternatively by integrated versus ecological technology. In a collection of varieties, the possibility of the experiment was feasible. At the same time, a comparison of the popularity of existing varieties was also actual. The organoleptic consumer’s tests rated the varieties, and the comparison with instrumentally raised “objective” data could be compared with the subjective ratings, which did not coincide with each other. This phenomenon was revealed in the rating of acid-sugar harmony, where the internationally used quality index of Thiault produced a different range of the samples then the consumer’s test. The same contradiction was observed between ratings of firmness and the data raised by penetrometer.
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The effect of summer pruning on solar radiation conditions in apple orchards
87-91.Views:225The aim of this study was to measure the solar radiation conditions in the tree and around the tree of pruned (summer pruning) and unpruned trees in an intensive apple orchard. When observing the daily trend of global radiation measured, there was a great reduction at midday hours due to the shadowing effect of the canopy. The reduction reached 70-75% before pruning, while after pruning it was only 20-25%. The relative radiation supply of the canopy space on cloudy days in the morning and late afternoon hours was higher than that of the sunny days. Mean differences between the day and night temperature reduced greatly in the period of intensive fruit colouration in August and September both in the plant stock and outside the plant stock.
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Fruit production and research in Hungary - An overview
7-11.Views:307Hungary is traditionally a food producer country. 63% of its total land area can be cultivated. Horticulture is one of the fundamental agricultural branches. The country has a moderate continental climate, with a mean temperature of 10 °C. The average hours of sunshine ranges 1,700 to 2,100 hours. Under the geographical condition in the Carpathian Basin the chemical composition of the fruits has a good harmony. The total fruit acreage is 97,000 ha with a crop of 800,000 to 900,000 tons yearly. In 1982 1,934,000 tons of fruit crop were produced since then it has decreased. The most important fruits are apple, European plum, sour cherry and raspberry. The percentage of apple reaches almost up to 60%. In the new plantings sour cherry, apple and black elderberry is popular. The most important fruit-producing region is situated at the North-eastern part of the country. More than 40% of Hungary's fruit production is concentrated there. In ranking the 2nd place is taken by fruit growing area in the middle of Hungary, where the production of stone fruits and small fruits has a considerable proportion.
In the 70s and 80s of last century there was a developed research structure and wide range of research activity in Hungary. From that time the research capacity has considerably decreased first of all in the field of technological development. The main research area is fruit breeding and variety evaluation.
Fruit scientists and fruit grower specialists are held together by the Hungarian. Society for Horticultural Sciences which has a membership in ISHS. Fruit researches and scientists having academic degree are belonged to the Horticultural Board of Hungarian Academy of Sciences.
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Comparative anatomical study of leaf tissues of scab resistant and susceptible apple cultivars
43-45.Views:178According to previous studies some anatomical features seem to be connected with resistance or susceptibility to scab caused by Venturia ineaqulis (Cke./Wint.) in case of a given cultivar. Study of leaf anatomy of three scab resistant (‘Prima’, ‘Florina’, MR–12) and two susceptible (‘Watson Jonathan’, ‘Golden Delicious Reinders’) apple cultivars have been made. Preserved preparations made of leaves has been studied by light microscope. Studied parameters were: thickness of leaf blade, thickness of palisade and spongy parenchyma, thickness of epidermal cells, thickness of the cuticle. By measuring leaf thickness and epidermal cell thickness visible differences appeared in certain cultivars, while most conspicuous difference has been shown in thickness of the cuticle.
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A preliminary study on some features of two new resistant apple cultivars in a multi-row planting system
11-14.Views:292The aim of this work was to report preliminary results on some features of two new resistant apple cultivars (cvs. ‘Galiwa’ and ‘Story Inored’) in multi-row system in the early bearing years of the orchard. Trees were planted in spring of 2015 in double row design (3.5 m+1.4 m x 1.0 m) with planting density of 5357 tree/ha. According to our results cv. ‘Story Inored’ presented more vigorous growth, than cv. ‘Galiwa’. Trees of cv. ‘Story Inored’ reached 2.7 m in the third year, but cv. ‘Galiwa’ could reach only 1.95 meter. Due to late spring frost 100% yield loss was observed in the second year. In 2017 cv. ‘Galiwa’ produced 5.3 kg/tree (25 fruit/tree), while cv. ‘Story Inored’ presented 7.7 kg/tree (50 fruit/tree). Average yield was 28.4 t/ha for cv. ‘Galiwa’, as ‘Story Inored’ reached 41.3 t/ha in the third year. Cultivar ‘Galiwa’ reached the required fruit size (79.1 mm), but its coloration was weaker (43% fruit surface color). Cultivar ‘Story Inored’ can be described with smaller fruit size (69 mm) and higher percentage of surface color (93%).
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Comparison of honeybee behaviour in blooming fruit plantations
147-151.Views:191Field observations were made on the fl ower visiting behaviour of honeybee foragers in commercial fruit plantations of apricot, Japanese plums, sour cherry, apple and pear. The number of inspected cultivars was 18. The intensity of fl ower visiting by honey bees was markedly different when data of different fruit species are compared. Most intense bee activity was registered on the Japanese plums, somewhat less on apricots, the intensity diminished signifi cantly with apples and pears. Our data presented on the honeybee visitation of Japanese plums can be regarded as new fi nding because no information has been available so far on the relative attractiveness of this fruit species compared to European fruit tree species. Japanese plums were somewhat more attractive to honeybees than apricot and much more attractive than sour cherry, apple and pear. The behaviour of honeybees as visiting the blooming trees displayed specifi c differences according to the fruit species (apricot, sour cherry, pear), which coincide largely with earlier results. It is notable that the fl ower visiting behaviour of honeybees on Japanese plums has been found to be fairly similar to the same on European plums.
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Results on mating disruption by sex pheromones against moth pests of apple in integrated and organic orchards
51-53.Views:167The study was aimed to study that how mating disruption by hand applied dispensers can reduce the number of damage caused by Cydia pomonella, Adoxophyes orana and Pandemis heparana in four integrated and organic apple orchards. In the first orchard (Gacsály), protection against moth caterpillars ensured by IPM and conventional production systems were equally good, but worse than that of the orchard part where mating disruption was applied by 1000 dispensers/ha. In second orchard (Nyírbogdány), the highest incidence of codling moth damage was measured in the hilly part (17%), while in the plot where 440 dispensers/ha pheromone dosage was applied, the damage incidence was 11%. The smallest damage incidence was at the flat part, where 666 dispensers /ha was applied. In the third and fourth orchards (Eperjeske), codling moth damage on fruits was below 7% in the larger and smaller orchards where 1000 dispensers/ha was applied. At Eperjeske, the codling moth damage increased by 32.3% in the field treated with Bacillus thuringiensis product but without using mating disruption. The results verified that the use of 1000 dispensers/ha as suggested by the manufactures is essential, especially in the first year of application. The results also suggested that better results can be achieved in flat areas and the larger plot size also enables a more efficient reduction of the damage.
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Scab resistance in Malus sp. progenies - inheritance and resistance stability
9-15.Views:133Susceptibility of progenies of some Ma/us species to apple scab was evaluated. Susceptibility of hybrid families was observed first in greenhouse after artificial inoculations by the suspension of the pathogen, Venturia inaequalis (Cooke) Wint., then in open field. The Hungarian type of Malus floribunda, which proved to be scab resistant in our examinations, has a positive effect on the segregation of progenies to resistant and susceptible individuals as a male parent, not depending on the rate of susceptibility of the other parent. However, the German type of Malus floribunda and Malus prunifolia act as dominant, highly susceptible parents.
Malus x purpurea and Malus baccata, which proved to be highly susceptible to apple scab in open field and in artificial inoculations, transmits its susceptibility only slightly to its progenies as a male parent.
According to our experiments, the inheritance of scab resistance of Malus floribunda cannot be considered as monogenic, as published by other authors.
Scab susceptibility of parents influences the segregation of progenies to resistant or susceptible individuals. The moderately scab susceptible `Idared' as female parent has a statistically proved positive effect on the segregation of its progenies.
More factors indicate the appearance of a new race of Venturia inaequalis (Cke.) Wint.: change of the symptoms in the greenhouse; considerable increase of susceptible individuals after greenhouse inoculation in 1997; more and more progenies of the male parent Malus floribunda became susceptible to scab in open field.
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Comparative economic analysis of superintensive and intensive apple orchards
45-48.Views:186The objective of thsi study was to compare economic aspects of superintensive and intensive apple production.
According to our results, conclusions and recommendations regarding the establishment of superintensive orchards are the followings: A yield of 60 tons per hectare in the average of the mature years provide a quite late payback, in this way yield losses should be avoided in the plantation of such a huge capital requirement. When yield losses happen, which cannot be avoided or may be avoided only in a limited way, up to 65 to 70 tons per hectare yield should be reached even in good years, in order to yield the average 60 tons per hectare in the long run. In this case yield losses from production technological mistakes must not be arisen. Inputs and professional expertise should be used in a maximum way in order to reach yields ensuring profitable production. Investment subsidies may ensure safer return. The return of a superintensive orchard from totally own sources may be risky under the domestic marketing conditions and selling prices, it is strongly uncertain. -
Characterization of quince (Cydonia oblonga Mill.) cultivars using SSR markers developed for apple
7-10.Views:306Quince (Cydonia oblongaMill.) is a minor fruit crop, which is primarily used for marmalade, jam and sauce.Very few quince cultivars are known all over the world and in many cases similar names are used for presumably different cultivars. The aim of the present study was to evaluate and characterize the genetic diversity of 36 quince cultivars and selections with SSR markers. Seven out of 8 SSR markers designed from apple sequences could successfully yield amplification also in quince cultivars. Number of alleles per locus ranged from 2 to 3 alleles. These allele numbers are quite low when compared to apple. It is supposed to be the consequence of a genetic bottleneck. In spite of the low allele number per locus, the 36 quince cultivars formed 30 different genotypes. The ratio of homozygosity was low, which might be coupled with the self-(in)compatibility phenotype of quinces. SSR markers proved unable to differentiate putatively closely related cultivars (e.g. ‘Bereczki’ and ‘Bereczki bôtermő’). In general, the level of polymorphism among the tested quince genotypes was much restricted due to the low allele number detected. However, it must be considered that the number of analysed SSR loci is not enough high to estimate the overall heterozygosity of the quince genome. Further experiments are needed and the SSR markers proved to be a reliable and useful tool for such analyses.
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New in vitro micrografting method for apple by sticking
79-83.Views:255The requirements for in vitro micrografting in apple are described. In vitro multiplicated shoots of cv. Royal Gala were the sources of rootstocks and scions after different pre-treatment, respectively. Oxidative browning of cut surfaces could be inhibited by the use of antioxidant mixture during grafting process. Scion base cut in v-shape was stuck by 1% agar-agar solution into the vertical slit of rootstock. There was no any displacement and the rate of fused and further developed grafts was 95 percent. Agar-agar between the rootstock and scion made the transport of different materials possible and hold the graft units together until the fusion took place. Fusion was proved also by histological studies. Some of in vitro micrografts were planted and acclimatisated and the survival was 100 percent.
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Apple allergens as affected by cultivation technolog and variental factors
91-93.Views:211Our objective was to study the effect of organic farming and area of cultivation on the allergen content of apple varieties by quantification of the major allergens (Mal d 1 and Mal d 2) using allergen-specific ELISA. The obtained results showed that except Idared IX. 29. most of examined varieties, when cultivated under organic farming conditions, contained lower level of Mal d1 allergen than those produced with integrated farming. The different varieties were found to vary substantially in theirMal d 1 allergen content. Furthermore, with some varieties the area of cultivation affected to some extent the content of Mal d 1 allergen.
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The effects of ACS (1-aminocyclopropane-l-carboxylate synthase) gene down regulation on ethylene production and fruit softening in transgenic apple
65-70.Views:113A detailed examination of the production of ethylene and other ripening parameters during storage period has been undertaken in transgenic apple fruits, where the ethylene biosynthesis was inhibited by antisense ACS (l-aminocyclopropane-l-carboxylate synthase) gene. Data indicate down regulation of ethylene production, softening and spoilage in some transgenic lines. In some cases ethylene production was inhibited for over 90 percent, considerable reduction of softening and spoilage was observed probably due to the reduced activity of cell wall degradable enzymes. ACS activity was also monitored during ripening. The fruits of the best transgenic lines could be stored for minimum 4-5 months longer under 5 °C cold room storage conditions and one month longer at normal room temperature. This molecular approach can provide an alternative way to replace the commonly used and costly atmospheric storage of fruits.
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Fruit coloration of apple cultivars
11-14.Views:332One of the most important parameter of apple cultivars is the scale and the intensity of the coloration of fruits. Nowadays among the bicolour cultivars those are the most saleable which have the highest and darkest fruit coloration. In our three years old study cultivars born with bud mutation and cross breading were examined, which have superior coloration peculiarities than their parental cultivars. According to our results among the observed cultivars the cvs. ’Gala Decarli-Fendeca’, ’Galaval’, ’Wilton’s Red Jonaprince’, ’Early Red One’ and ’Red Cap Valtod’ reached favourable high fruit surface colour (80-100%) regardless the vintage effect. Concerning the colour intensity, that is the darkness of the red colour most of the cultivars (‘Gala Venus Fengal’, ‘Gala Fendeca-Fendeca’, ‘Galaval’, ‘Jeromine’, ‘Red Topaz’, ‘Early Red One’, ‘Crimson Crisp’, ‘Wilton’s Red Jonaprince’) showed excellent values (4,5-5,0). Based on the fruit surface colour and the fruit colour intensity a new index number, the fruit coloration index was created by us, which describes the coloration features in a complex way.