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  • Industrial Utilization of Grasses
    131-134
    Views:
    64

    The Agricultural Research and Development Institute P.U.C., Szarvas – realizing the importance of using the biomass in a number of ways – was one of the first in Europe, who began the breeding of grasses suitable for industrial utilization. The possible areas of using the energygrass – which could be called industrial grass – are the following:
    – Energetical use
    – Paper industry use
    – Utilization as an industrial fibrematerial
    – Use for feeding
    The aim of the breeding programme: developing the kinds of grass, which yields big drymaterial mass and suitable for industrial utilization. These kind of grass, taking soil-utilization, economicality and environmental protection, offer and ensure new marketing perspectives and employment facilities for the disadvantageous areas.

  • Polymorphism of the bovine GH and LEP genes in a population of Slovak spotted bulls
    19-23
    Views:
    91

    The aim of this study was the detection of polymorphism in the bovine growth hormone and leptin genes using the PCR-RFLP method. A
    polymorphic site of the growth hormone gene (Alul loci) that results in amino acid change at position 127 of the protein chain (leucine, L to
    valine, V) has been linked to differences in circulating metabolites, metabolic hormones and to milk yield. The polymorphism in bovine leptin
    gene is situated in the intron between two exons, which results in an amino acid change at position 2059 of the protein chain (cytosine, C to
    thymine, T). The polymorphisms were studied in a group of 58 bulls of the Slovak spotted breed. A strategy employing PCR was used to amplify 428 bp (GH gene) and 422 bp (LEP gene) products from blood samples. Digestion of PCR products with restriction enzymes AluI and Sau3AI revealed alleles: L and V; A and B for GH gene and LEP gene, respectively. The growth hormone gene is a candidate gene for body weight gain in cattle, since it plays a fundamental role in growth regulation. Leptin plays an important role in the regulation of feed intake, energy metabolism, growth and reproduction of cattle; therefore, animals with higher leptin gene expression will probably have lower daily weight gain than others with similar forage offer and nutritional condition and will also likely have longer calving intervals. 

  • ‘Kindergarten’ keeping-system in farrowing house: effect the socialization of piglets on weight performances, fecal cortisol metabolite level and post-weaning behavior
    167-174
    Views:
    151

    This study aimed to examine whether litters’ let-together system before weaning (‘kindergarten-system’) has any stress effect and effect on post-weaning behavior, respectively. One week before weaning piglets were socialized by removing four adjacent farrowing crate walls. After weaning, piglets from the same experimental groups were housed in the same rearing crates. The piglet’s body weight was measured at birth than weekly till the 7th life-week. Fecal cortisol metabolites were measured for evaluation of the adrenocortical activity. Personal observation and evaluation were carried out for behavioral analyses after weaning. There were no significant differences in weight development and cortisol metabolite levels between the control and experimental groups. Behavioral analysis showed that pigs grow up in a socialized system, rapidly evolve ranking in the rearing crates, and the self-maintenance and social behavior were more pronounced. In control groups, the activity involving movement (exploring, walking, and running) was much more decisive. Recent work suggested the beneficial effect of the ‘kindergarten’ system in the farrowing house in animal welfare aspects.

  • Using phenotypic correlations in the breeding evaluation of Hungarian Sport Horse mares
    38-43
    Views:
    103

    The aim of this paper is to analyse phenotypic relationships between self performance test (SPT) parameters of Hungarian Sport Horse mares. Establishing these relationships may contribute to develop a breeding value estimation model. Data were given by the Association of Hungarian Sport Horse Breeders.
    The analysis is based on the SPT results of 3 and 4 year old mares from 1993 to 2004. Our aim is to determine practically explainable correlations among conformational traits (type, head, neck, saddle region, frame, forelegs, hind legs, regularity of movement, impulsion and elasticity of movement, overall impression); free jumping performance (jumping style, jumping ability, observation during training) and movement analysis traits (walk, trot, canter, overall impression, test rider’s score). A further aim is to determine correlations between free jumping performance and movement analysis traits.

  • Sporthorse performance testing in eventing by own and progeny performance
    49-56
    Views:
    125

    The aim of the study was to evaluate the Hungarian Sporthorse population based on eventing competition performance. The database contained the results of 792 horses and 449 riders between 2000 and 2006. The eventing results were gathered from Hungary and other European countries. Blom transformed ranks were used to evaluate the sport performance.Three models were fitted to the Blom scores. Evaluating all the competition categories at the same time weighted Blom scores were used according to the difficulty of the category. The linear mixed model included fixed effects for age, sex, breeder, owner, location, year; and random effects for animal and rider. Horses from the database were judged by their own performance, and stallions were investigated by performance of their progenies on the basis of descriptive statistics of Blom scores and weighted Blom scores. Breeding values of eventing performance were predicted. To improve the reliability of breeding values, more progenies should be
    used in eventing competitions. 

  • Evaluation of egg quality parameters of two Hungarian ostrich populations
    51-57
    Views:
    200

    The aim of our study was to evaluate the quality parameters, porosity and weight loss of eggs deriving from the two most significant ostrich farms in Hungary. Quality parameters included weight, length, width, shape index, egg volume, surface area, circumference and shell volume.  The effect of storage conditions in both farms and the incubation technology on egg weight loss in farm “A” were also examined.  The research objective was to impart a comprehensive knowledge on egg quality parameters of the main ostrich populations in Hungary and to compare the trios and the farms with each other and the international literature. We could reveal significant differences between trios in all egg quality traits. In conclusion, the shorter and the narrower the eggs were, the more spherical shape they had.  Narrower eggs showed smaller surface area, volume, circumference and shell volume and vice versa. Eggs from farm “B” indicated significantly greater width, shape index, surface area, circumference and shell volume than farm “A”. A significant difference was observed in weight loss during storage between the farms.  Weight loss in farm “A” was a multiple of farm “B”. In farm “B” there was a weak, positive correlation between storage period and weight loss (r=0.22, P≤0.05), in farm “A” it was not significant (P=0.52). There was no relationship between the initial egg weight and weight loss either in farm “A” or farm “B” (P=0,21, P=0,69). A slight positive correlation could be noted between egg porosity and weight loss (r=0.24, P≤0.05). Pores count presented here was less than the international results. Poultry eggs contain the most pores at the blunt end, less via the equator and the least at the pointed end. In ostrich egg we found more pores via the equator against the blunt end. To draw more precise conclusions, further investigation should be carried out on porosity. Considering the fact that the length of storage period and the weight loss during incubation are in strict correlation with hatchability, we intend to extend our research aims to these traits.

  • Lambing Frequency in the Ile de France, Suffolk and Babolna TETRA Stocks of Babolna c.o., Szendrő
    29-32
    Views:
    115

    Lambing frequency of three different genotypes was evaluated in the breeding stock of Babolna c.o. Szendrő. Ile de France, Suffolk and Babolna TETRA breeding ewes gave the data for the further evaluation. The main aim of this research was to find out if there is any significant effect of selected parameters (registration class, lambing season, lambing type and the years) on the lambing frequency, the period between consecutive lambings. Production parameters of 9,471 Ile de France, 1,244 Suffolk and 7,618 Babolna TETRA ewes were collected and processed. The analysis covered the period between the years 1989-1999. It was concluded that not all of the evaluated parameters had a significant effect on lambing frequency. Not all of the genotypes showed significant differences, according to the examined parameters.

  • Origin, history and utilization possibilities of Carpathian Braunvieh at Hungary
    15-20
    Views:
    166

    The Carpathian Braunvieh was established by the cross-breeding of Braunvieh and the small dun mountain breeds of the Carpathian basin. It has been breeding in Hungary about 100 years before and still living in Transcarpathia and Transylvania, but only in very mixed populations. Since it was a characteristic native cattle breed of the country, it should be retain for future generations and re-establish in Hungary. Our primary aim is to preserve the breed’s genetic resources and - if appropriate - to acclaim it as an indigenous breed. If there are no longer residuals of the old type Carpathian Braunvieh, it is still worth to preserve as a local variety, because of its many favourable features. Above all, it’s our duty to maintain the breed, because neither Transcarpathia nor Transylvania has a breeding programme for it.

  • Comparison of RAPD and AFLP Analysis in Some Maize (Zea mays L.) Lines and Hybrids
    3-7
    Views:
    88

    The use of molecular markers to enhance plant breeding efforts is being widely studied. DNA-based fingerprinting technologies (RAPD and AFLP) have proven useful in genetic similarity studies. We estimated different maize (Zea mays L.) inbred lines and hybrids originated from mutant ones based on their genetic differences.
    We carried out RAPD analysis with different primers and the 707 (CCCAACACCC) and 792 (CAACCCACAC) primers with 50% similarities provided quite good DNA fragments. By applying the DNA based-AFLP technique, we had very dense DNA fingerprinting. We differentiated 15-32 polymorphic bands, the highest number of bands were found in P-T/H-CA (32). AFLP seems to be the more efficient method of comparing genetic similarities/differences among different genotypes.

  • Ovarian performance of Barbados Blackbelly and Dorper crossbred ewes in the mirror of body conditions
    23-26
    Views:
    77

    The aim of examination was to detect the puberty and to follow the ovarian cycle of Barbados Blackbelly×Dorper ewes in connection with their body conditions and the effects of meteorological parameters. The blood samples had been taken from 17th of November 2008 untill 08th of June 2009 for analyzing the progesteron concentrations, establishing whether they showed perpetual ovarian performance or not. The end of examination
    was timed due to releasing the ram in middle of May 2009.
    The daily avarage bodyweight-growth is well featured by the bodyweight tendence. The body conditions were evaluated by ultrasound tissue examination. 
    During the full length of the examination the avarage daily temperature, the avarage humidity level and also the number of daily sunlighted hours were served by the Agrometeorological Observatory of the University of Debrecen.
    The bodyweight tendence and the meteorological parameters were correlated to the breeding season of ewes. In conclusion the bodyweights and their tendences at the young age and the body condition parameters measured at certain anatomical areas can influence the length of the breeding season of the ewes. However the number of daily avarage sunlighted hours, the daily avarage temperature and also the humidity did not effect the cyclic ovarian perfomance of the Barbados Blackbelly×Dorper ewes in Hungary.

  • Study on the cold tolerance of maize (Zea mays L.) inbred lines in Phytotron
    41-45
    Views:
    51

    Maize has come a long way from the tropics to the temperate zone. In the beginning, the spreading of maize was prevented by its sensitivity to cold. Improved cold tolerance at germination is one of the most important conditions for early sowing. The advantage of cold tolerant hybrids is that they can be sown earlier, allowing longer growing seasons and higher yields, due to the fact that the most sensitive period in terms of water requirements, flowering, takes place earlier, i.e. before the onset of summer drought and heat.

    In Martonvásár, continuous research is carried out to improve the cold tolerance of maize. In the present experiment, the cold tolerance of 30 genetically different maize inbred lines was investigated in a Phytotron climate chamber (PGV-36). The aim of our research is to identify cold tolerant lines that can be used as parental components to produce proper cold tolerant hybrids and/or as sources of starting materials for new cold tolerant inbred lines. After observing and evaluating changes in phenological traits under cold-test, the results of the cold-tolerance traits of interest have been used to highlight several inbred lines that could be good starting materials for further research on genetic selection for cold tolerance.

  • Results of Giltsrearing in Outdoor Production System
    8-11
    Views:
    68

    The applied technology is an alternative approach to pigkeeping-systems. An outdoor pig production breeding sows are kept at pasture either year-round or in a certain period of the year. The important equipments of outdoor pig production are farrowing or grouping sows inhuts, which protect pigs against the effects of extreme weather, and electric fences, which surround and divide the pasture. Concentrate feed can be fed from the ground or from feeders which are made of steel or timber. One of main advantages of this pig keeping system is the total mobilizable keeping technology.
    Within the scope of the study we are performing an experiment to make a comparison between coventional system and free range sows keeping technology. Pannonhybrid F1 gilts were used in this experiment, 28 gilts were kept on pasture all day and 28 gilts are kept in conventional, indoor system.
    In this work the results of gilts-rearing are presentated as a part of our two-years experiment.

  • The possibilities of biomass utilization in the field of spatial development
    59-63
    Views:
    63

    By joining the European Union, Hungary made a commitment to increasingly utilize renewable energy sources. Keeping in view the agricultural circumstances of Hungary, we can state that biomass utilization has, overall, the greatest range of possibilities to use its products as ‘energy sources’. Biomass raw materials are useful in meeting emission control regulations for environment protection and to reduce climate change. The role of biomass production in spatial and rural development and in rural employment, and also in the decrease of Hungary’s energy dependence, supports development in biomass energy use. My aim in studying biomass utilization is the creation of a micro-regional level, decentralised, agro-energetic system-model for the use of plant and animal biomass for energy purposes, whether naturally or by anthropogenic activity, on agro- and sylvicultural areas, and on animal breeding farms. This model simulation method serves as a planning base for policy-makers during the spatial planning processes. The examination is presently in the stage of data collection. The collection of the basic data of the examination area and the purchase of the necessary equipment, materials and maps has already started. Contact has been made with local stakeholders, municipalities, municipality associations and micro-region managers.

  • Seasonal variations in somereproductive parameters of Dorper Rams in Hungary
    17-20
    Views:
    235

    The purpose of the present study was to test the hypothesis that season has an affect on semen quality and scrotal circumference of Dorper rams. The experiment was carried out with six Dorper rams aged between 15 and 18 month. Semen samples were collected with artificial vagina and volume, concentration (x 109/ml), total sperm number/ejaculate (x 109), mass motility (0–5), progressive motility (%), scrotal circumference (cm) was observed. Significant differences (P<0.05) were observed in concentration, total sperm number/ejaculate, scrotal circumference in different seasons. Volume was the highest in autumn (1.4±0.5 ml) and the lowest in the spring (1.3±0.4 ml). Concentration of semen was lower in spring (2.6±1.5 x 109) and summer (3.3±1.5 x 109) as compared to fall (4.1±1.1 x 109) (P<0.05). Regarding total sperm number/ejaculate (x 109), scrotal circumference (cm) all the seasons differed significantly (P<0.05), although the season had no effect on mass motility and progressive individual motility (P<0.05). In conclusion the present study showed that semen quality parameters and scrotal circumference of Dorper rams were better in autumn than in the other sea- sons.

  • Study of the Interaction Between Common Carp (Cyprinus carpio) and Silver Crucian Carp (Carassius auratus gibelio Bloch)
    9-12
    Views:
    99

    Some of the main questions in the fish farming are the good manuring and the success of the feeding. For the best result is very important that only those fishes take place in the fishponds what we want. It seems to be easy but difficult to do. One of the main challenges of the successful fish production in fishponds, is to rid ponds of the silver crucian carp. In the course of the experiment, carp fry were stocked in 5 nursing ponds at the same stocking rate, with different (0-, 50-, 100-, 150-, 200%) stocking rates of silver crucian carp fry, respectively. During the rearing season, weekly test fishings were made to control the growth of the fishes. At the end of the experiment, the yield/hectar and the growth rates were calculated. The experiment proved that the presence of the silver crucian carp in the fishponds causes lower growth rates and productivity in common carp. In the control pond (without silver crucian carp), the average weight of the common carp was 25 g and the yield/hectar was over 900 kg, while in the pond with the most silver crucian carp, the average weight of the common carp was 9 g and the yield/hectar was only 158 kg.

  • The analysing of value measuring features of Hungarian speckled hen
    17-23
    Views:
    34

    Our College has been dealing with the crossbreeding of two Hungarian speckled hen breeds, the bare-neck and the featheredneck, to maintain the breeds since 1977. Apart from the gene preservation, we endeavour to find the best way for the production-purpose utilisation of the speckled hen stock. On the basis of our experiments the laying hens can be used in small-scale egg production. As for meat production, these pure-breds cannot compete with the various types of the colour-feathered hybrid constructions available in Hungary. On the basis of the results of the cross-breeding with cock-lines of various hybrid types for direct production purposes we can conclude that the Hungarian speckled hen is suitable as a mother line to produce meat-type chickens in alternative breeding systems.

  • Study of the Interaction Between Common Carp (Cyprinus carpio L.) and Silver Crucian Carp (Carassius auratus gibelio B.)
    16-19
    Views:
    81

    During the last few years, the spread of the silver crucian carp caused significant problems in pond fish culture. One of the main challenges of the successful fish production in fishponds, is to rid ponds of the silver crucian carp. In the course of the experiment, carp fry were stocked in 5 nursing ponds at the same stocking rate, with different (0-, 50-, 100-, 150-, 200%) stocking rates of silver crucian carp fry, respectively. During the breeding season, weekly test fishings were made to examine the growth of the fishes. At the end of the experiment, the yield/hectar and the growth rates were calculated. The experiment proved that the presence of the silver crucian carp in the fishponds causes lower productivity and growth rates in common carp. In the control pond (with no silver crucian carp), the average weight of the common carp was over 20 g and the yield/hectar was over 700 kg, while in the pond with the most silver crucian carp, the average weight of the common carp was 4.5 g and the yield/hectar was only 123 kg.

  • The evaluation of body condition scoring of Holstein-Friesian cows
    57-61
    Views:
    105

    We carried out the examinations on a farm of 500 cows where We examined the body condition of the stock every month. We recorded the body condition data together with the milk production and calving data. We analysed the daily milk production as well as the body condition. During the examinations we sought an answer how the body condition and the production level change during the lactation period.

  • Molecular genetic investigations for increasing the production results of farm animals
    92-96
    Views:
    72

    The author analysed the publications related to molecular genetics presented at the 51st annual meeting of the European Association for Animal Production. Her aim was to make the latest molecular genetic results known and to emphasise that this discipline plays an increasingly important role in research and the practical fields of animal breeding and production.

  • Investigation of genetic diversity in irradiated maize lines and its relation to hybrid performance
    20-26
    Views:
    153

    Knowledge of genetic diversity among available parental lines is fundamental for successful hybrid maize breeding. The aims of this study were to estimate (1) genetic similarity (GS) and genetic distance (GD) (based on Jaccard index) in four maize inbreed lines; (2) to classify the lines according to their GD and GS; (3) to determine hybrid performance based on GD and heterosis for yield ability in 4x4 full diallel system. We used morphological description and AFLP (amplified fragment length polymorphisms) for estimation genetic polymorphism in four maize inbred lines. We estimated the applicability of genetic similarity in SC and reciproc hybrids for prediction of their performance.
    Three primer combinations were used to obtain AFLP markers, producing 207 bands, 70 of whit were polimorphic. The dendogram based on genetic similarities (GS) and genetic distance (GD) and morphological description separated four inbred lines into well-defined groups. Morphological description just with AFLP analysis showed reliable results. In view of genetic distance, the UDL 1 line and their linear and reciprocal crosses showed significant heterosis effect, which was confirmed by heterosis calculation based on grain yield.

  • Analyzing the Efficiency of Dairy Farms by Using the Method of Data Envelopment Analysis (DEA)
    17-21
    Views:
    89

    In Hungary the dairy sector is in a long-term critical period, the stock has been in constant decline. The consumption of milk and dairy products in Hungary is slightly rising compared to the world tendency, and it is fallen behind the level in 1990. The milk consumption per capita in 2006 was with 75 liters less than the EU-15 average. Dairy enterprise is a very risky activity: the profitability of the enterprise is affected by the fluctuation of feed and animal health products prices from the side of inputs, and by the fluctuation of end-product prices. Under these circumstances it is vital for the
    cattle breeders, in order to survive, to harness the reserves in the breeding as effectively as possible. In our research we made a multi-faceted efficiency analysis of an agricultural holding’s three dairy farms. The chosen method for the analysis was Data Envelopment Analysis (DEA). The
    selection of the method is justified by the fact that there is not such a reliable database by which we could define production functions, and that DEA makes possible to manage several inputs and outputs, i.e. multiple decision problems, simultaneously. By using DEA the sources that causes shortfalls can be identified, analyzed and quantified on farms that does not operate efficiently, thus it can help the corporate decision support successfully. In the model inputs are the cost data per one liter milk – feed, medicinal product use, logistic costs -, and the main parameters
    concerning the keeping and rearing. Outputs are indicators concerning milk production, milk quality and others. We prepared the model in MS Excel, the linear programming model series were programmed by Visual Basic. After solving the model, in light of the shadow prices we can determine why either of the farms is not efficient.

  • Some Practical and Biotechnological Methods for Improving Reproduction Traits in Sheep
    15-20
    Views:
    110

    However, reproduction in sheep is seasonal, many breeds of sheep are able to mate not only in autumn, but out-of-season as well. The main factor determining seasonality is the photoperiod, but other factors can influence reproductive pattern, such as genetics, management practices and social cues. The fertility of spring and early summer breeding is usually lower; this imposes the need for alternative methods (e.g. hormonal treatments, biotechnological practice), to increase the conception rate.
    The author summarize the main practical techniques and biotechnological methods for controlling reproduction completed with some own experimental results in connection with different topics.

  • Effect of floor type and bedding system on sows lifespan
    155-160
    Views:
    105

    The paper studies two nucleus pig farms in the Great Plain region of Hungary which have same management, same feeding system but different breeding technology (Farm A had solid floor with straw and Farm B had slatted floor without straw). The genetics of sows were crossbred Dutch Large White and Dutch Landrace. The comparative examination was based on the causes of culling according to the different floor type. To the analysis of culling reasons was applied the methods of survival analysis.

    In the analysis there were used the Kaplan-Meier method and the Cox Proportional Hazard model to the estimate the difference between the farms. The results of survival analysis showed significant difference (p<0.05) between the lifespan of sows that caused the difference between the survival probabilities of sows culled due to lameness. Furthermore there was showed that the sows kept on solid floor with straw have stayed in production longer than on slatted floor without straw. The hazard ratio was 1.434 that means that the culling due to lameness is 1.434 times higher by the slatted floor without straw facing by solid floor with straw.

  • Effect of different n-6/n-3 fatty acid proportion oil sources on reproduction performance and fatty acid profile of milk in modern genotype sows - Pleminary results: Preliminary results
    121-128
    Views:
    130

    This study was conducted to investigate the effects of supplemental n-6 and n-3 fatty acids on sow’s milk fatty acid composition during the lactation period and on reproductive efficiency of sows in the subsequent gestation period. Data were collected on a total of 213 DanBred sows (108 control and 105 experimental) representing parity of 2–7, respectively. Control and experimental sows were placed in the same housing conditions during lactation and gestation period. Control group received 6.3 g of sunflower oil (SO) per kg feed as n-6 fatty acid supplementation, whilst experimental animals received the same amount of fish oil (FO) as n-3 fatty acid source. Diets were corn meal-extracted soybean meal based. The experiment was conducted in one replication as being a part of a longer and more comprehensive trial. It was found that the consumed long-chain polyunsaturated fatty acids (LC-PUFAs) appeared in the sow’s milk and changed its fatty acid profile. With this alteration, the n-6/n-3 ratio of experimental (FO) sows’ milk were narrower than in SO group (SO: 13.82 vs. FO: 5.89). The benefits of n-3 fatty acids supplementation were evident for the subsequent reproduction cycle, when experimental sows heated more reliable and earlier than control (weaning to oestrus interval: SO: 5.86 vs. FO: 4.48 days). Only 2.33% of experimental sows (FO) did not heat within 7 days after weaning, but this was 12.36% in the control group (SO). The present study requires further research to evaluate the effect of n-3 fatty acids on maintenance of pregnancy and improved subsequent litter size.

  • Investigation of Potato (Solanum tuberosum L.) Salt Tolerance and Callus Induction in vitro
    51-55
    Views:
    90

    Potato production plays an important role in Hungary and the other countries of Europe. Consumption of potato products has increased to a large extent during the past several years. We can satisfy market demands with high quality and virus-free varieties.
    Results of potato production depend on tolerance/resistance to abiotic stresses. In many cases, increased concentration of NaCl causes yield loss. Selection of salt tolerant varieties proved to be a difficult problem. Nowadays, the salt tolerance of potato varieties can be determined by cell/tissue/ protoplast techniques. Somaclonal variation provides a great potential for selection of lines resistant to salt stress. In vitro shoots and callus, derived plantlets selected for salt tolerance/resistance provide material for micropropagation.
    In vitro shoot development of potato (Solanum tuberosum L. cv. Kuroda) was investigated under salt stress (40 mM, 80 mM, 120 mM NaCl) conditions. Shoot heights of plantlets cultured under salt conditions were lower than the control through the investigation. However, the shoot development of plantlets originated from in vitro meristems was almost at the same level as the control under 40 mM NaCl concentration.
    There was no significant difference in the in vitro biomass production between control and treatment with 40 mM NaCl concentration. We measured a significant decrease in dry-matter mass under 120 mM NaCl concentration. There is a need for more investigation of different genotypes and for a conclusion as to whether in vitro tolerance could occur under in vivo circumstances in plants originated from somaclones as well.
    Under in vitro conditions, we investigated shoot and leaf callus initiation using different culture media with different 2,4-D concentrations. Under dark conditions, callus induction of shoot/leaf decreased as the 2,4-D concentrations increased.
    In light conditions, there was a little callus induction, while callus initiation from the shoot from 5 μM to 12 μM 2,4-D concentration showed a significant increase