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Mass occurrence of a Phoma-like fungus on common ragweed (Ambrosia artemisiifolia) in Hajdúság region, East Hungary
55-60Views:432Common ragweed (Ambrosia artemisiifolia L.) is one of the most important, allergenic weed species in Hungary. A. artemisiifolia invades both a broad range of often disturbed areas (brownfields) and either undisturbed ones like waste lands, roadsides, riverbanks and railway tracks. In field crops it can cause considerable yield losses mainly in sunflower, maize, cereals and soybean. In Hungary many inhabitants suffer from allergy caused by Ambrosia pollen which results a serious human-health risk. The aim of the control is to prevent flowering and seed propagation of A. artemisiifolia. Until now the occurrence of numerous pathogenic fungi which attack common ragweed plants have been identified in Hungary, however there is not yet available biological weed control program because of shortage in acceptable effectiveness, and endangering cultural plant species. During our weed surveys in the region of Hajdúság (East-Hungary) we found numerous common ragweed plants showing heavy necrotic lesions on leaves and stems. The objective of this study was to identify the fungus which was isolated from diseased tissues of common ragweed (A. artemisiifolia). The identification of fungus based on morphological characters of colonies and features of conidia and chlamydospores developed on malt extract agar (MEA) plates. After examination of axenic cultures we revealed that the fungus isolated from the leaves ands stems of common ragweed was a Phoma-like species.
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Molecular Biological Approach of Crocus sativus L. and its Allies
112-115Views:618The hay saffron (Crocus sativus L.) is a sterile triploid plant, known in human culture only, with no fertile seeds produced. The origin of saffron is still a mist, however it is assumed to be an autopoliploid mutant or a hybrid. The recent classification and most of the former taxonomic publications define C. sativus to be derived from C. cartwrightianus, a wild species. Because of the sterility of hay saffron it seemed to be reasonable to apply molecular biological methods to complete classical taxonomic studies in examining its relations. The DNA polymorphism based AFLP method has confirmed the close relationship between these species.
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The effect of and interaction between the biological bases and the agrotechnical factors on maize yield
83-87Views:278The effect of and interaction between the biological bases and the agrotechnical factors on maize yield In our research, we examined the effect of the hybrid, the nutrient supply, the number of plants and the abiotic factors (temperature, amount of precipitation) on the yield, crop quality and yield stability of maize. We devoted special attention to the natural nutrient utilization ability and fertilizer reaction of maize. The experiment took place in Hajdúszoboszló on chernozem soil, on a nearly 8 ha field. The size of one plot was 206 m2; therefore, this experiment was half-industrial. We tested six hybrids with different genetic characteristics and growing seasons.We analysed the correlation between the nutrient supply and the yield of maize hybrids with a control treatment (treatment without fertilization) and with N 80, P2O5 60, K2O 70 kg ha-1 and N 160, P2O5 120, K2O 140 kg ha-1 fertilizer treatments. The yield increasing effect of the fertilizer also depended on the number of plants per hectare to a great extent. The number of plants of the six tested hybrids was 60, 70, and 80 thousand plants ha-1.In 2015, the highest yield was produced by hybrid P9241 with N80+PK and 70 thousand plants per hectare. With the N160+PK fertilizer dosage, the same hybrid responded the best, followed by hybrids P9486 and DKC4717. Using the same fertilizer treatment, the 80 thousand plants per hectare population density resulted in decrease in the yield with most of the examined hybrids. In 2016, with the increase in the number of plants per hectare, even with non-fertilised treatment (control treatment), the yield could be increased in the case of each hybrid.Averaged over the different hybrids and fertilizer treatments, applying 80 thousand plants ha-1 instead of 60 thousand resulted in 1.0 ha-1 yield increase. In 2017, the number of plants had a slighter effect. With N160+PK treatment, in most cases no significant difference can be observed. The value of LSD5%: plant number: 0.20 t ha-1, hybrid: 0.28 t ha-1, interaction: 0.48 t ha-1. With N160+PK treatment, the hybrids produced yields between 10.07 and 12.45 t ha-1. When examining the three years in the average of the number of plants, with treatment without fertilisation, the average yield of hybrids reached 7.53 t ha-1. With N80+PK treatment, this value was 9.71 t ha-1 and with doubling the fertilizer dosage, this value increased to 10.42 t ha-1. No economic profit was gained as a result of applying double dosage of fertilizer; therefore, the N80+PK dosage can be considered ideal. -
Soil Biological Activity within Integrated and Ecological Management of Soil
47-52Views:176The effects of the integrated (IS) and ecological (ES) management of soil on chosen parameters of soil biological activity were investigated in the period 1999-2000. The following characteristics were determined: biomass of microorganisms (Cmic), dehydrogenase activity (DHA), an amount of potentially mineralizable nitrogen (Nbiol), and nitrification intensity. Soil samples were collected from a stationary field experiment established in 1990 on gley brown soil at the Experimental Station of Slovak Agricultural University, Nitra. For each field with a different crop rotations two fertilization treatments were selected: (a) no fertilization and (b) use of manure for silage maize and, within IS, also mineral fertilizers. There was a statistically significant difference at α = 0.05 in the amount of biologically released nitrogen (Nbiol) between both systems and in the nitrification intensity in favour of ES. A higher amount of microbial biomass (Cmic) was noted for ES but without statistical significance. Cultivated crops and the timing of soil sampling were found to have the greatest effect on all the parameters observed in individual experimental years and within the two systems of soil management.
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Influence of mammal fossorial activity on bearing-out some chemical elements on up of soil cover
117-120Views:222Fossorial activity of mammals is conductive to trace of microelements from more deep soil horizons into zone of its active involving to biological cycle. As a result of researches have established the mostly intensive migration of micro- and macroelements that is goes at the expense of mammals fossorial activity it is typical for humid gully lime-and-ash with oak wood. A middle position in speed of entering chemical elements is belonging to artificial oak wood in the watershed and humid lime-and-ash with oak wood in floodplain. Mostly slow migration in speed of entering elements is typical for middle-dry pine wood on sandy terrace.
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Biological potential of plant pathogenic fungi on weeds: A mini-review essay
59-66Views:653The invasion of weeds into productive areas has substantial negative effects on native ecosystems as well as agricultural production systems globally. Consequently, the task of maintaining or restoring these systems will become increasingly challenging without consistent, ongoing management efforts. The intensifying emergence of herbicide resistance in numerous weed species, coupled with the unintended pollution caused by synthetic herbicides, underscores the growing necessity for alternative, environmentally friendly, and sustainable management techniques, such as the utilisation of bioherbicides. Plant pathogenic microbes play an important role in biologically management of weeds, with the utilization of plant pathogenic fungi emerging as a promising area of study for novel research trends aimed at weed management without reliance of herbicides and to mitigate environmental pollution. A potential solution to decreasing pesticide usage involves the development of bioherbicides containing fungal active ingredients. Among the most commonly utilised fungi in bioherbicides are genera like Alternaria, Colletotrichum, Cercospora, Fusarium, Phomopsis, Phytophthora, Phoma, and Puccinia. Increased weed resistance to herbicides has influenced new strategies for weed management, with some fungi from genera such as Colletotrichum and Phoma already employed for weed control. Nonetheless, it is evident from reviews that further research is imperative in this domain, with particular emphasis on analysing the efficacy of each plant pathogenic fungi.
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Optimization of inductively coupled plasma mass spectrometer parameter’s to measuring arsenic and selenium
59-64Views:739In the last decades an increased interest has been evolved about arsenic and selenium. The aim is to understand the environmental, agricultural and biological role of the these elements. In case of arsenic the mayor reasons are the relatively high concentration of arsenic in marine biota (mg kg-1) and the arsenic contaminated drinking waterbases of some Asian countries besides Hungary. The toxicity of higher level selenium content is also known, nevertheless selenium is essential for some biological functions. Considering its esssentiality, in our country the insufficient selenium intake rate couse lack of selenium. Measuring the concentrations of these elements are cruital but not satisfactory information, but the speciation, that is the form of an element presented in a sample is also required.
In both cases the most suitable method to determine concentration is the inductively coupled plasma mass spectrimetry. My objective was to optimase the changeable parameters of the ICP-MS for reaching the lowest (the best) detection limit. For this porpuse I have investigated the effect of parameter change on nett signal intensity and relative signal intensity. With the optimased parameter settings the limit of detection for arsenic and selenium were determined, which are 0,032 ng cm-3 for arsenic, and 0,097 ng cm-3 for
selenium. -
The olive (Olea europaea) and the almond (Prunus amygdalus) related phytonutrients, and the associated health-promoting biological effects, a review
11-24Views:444With the increasing attention to the health promoting activities of the bioactive compounds from some plants, many researchers are focusing on the biological potential and mechanisms of certain cultivated plant species. In this review, we survey the olive and almond based extracts specific phytoconstituents and their associated health promoting effects that have been evaluated in experimental and clinical studies.
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Compost application in integrated and organic fruit cultivation
135-139Views:296Nowadays the success vegetable and fruit production are unimaginable without regular nutrient management. One of the ways to supply the required nutrients in an environmentally friendly way is the application of composts, which is less widespread so far. Compost doses were applied in biological and integrated apple orchards in cooperation with the Institute of Horticultural Science in the years 2010 and 2011. Different changes were resulted by the compost treatments in the examined parameters in case of both apple varieties (Golden Delicious and Pinova). There is no clear effect of compost on the changes of ash-, total acids, Vitamin C and the sugar content until now, because the improvement of the nutritional indicators takes more time. However significant differences were observed after one year between the apple varieties. Higher ash-, total acids and sugar content were measured in case of the apple variety Pinova, while the measurements showed higher Vitamin C content in case of the variety Golden Delicious.
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Biological control of sweet chestnut on Pécsbánya, Hungary
77-81Views:490The supervision of plant hygiene of sweet chestnut grove on Pécsbánya (South Hungary) started more than four years ago. Hypovirulent strains were applied as a biological process to control Cryphonectria parasitica fungus which causes the chestnut blight disease. By now the performed interventions have shown obvious results, the vitality of the trees has greatly improved, the amount of harvested nuts is increasing, and the hypovirulent strain has been spreading within the area. During plant health inspection the galls of chestnut gall wasp (Dryocosmus kuriphilus) was found in the year of 2015, which is the obvious symptom of new occurrence of the pest. The pest was eradicated by destroy galls, which allows taking out of consideration the damage by now in this area.
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The phosphate state and biochemical mobilization of phosphorus compounds in arboreal plants’ soils
95-98Views:187Some indices of the phosphoric fractions of primery degraded soils, which are formed separate areas of technogenic landscapes, on a spoil-bank of iron-ore mine in the near of Kryvyi Rig, under act of lignosa, which are used for biological recultivation of degraded soils are investigated. Maintenance of mineral phosphates and features of organic phosphorus accumulation are set in soil under arboreal planting. Nutrient supply of plants is enhanced by mobile phosphates and their dynamics during vegetation period. Activity of alkaline and acid phosphatase enzymes are concerned also. On the basis of the soil enzymes activity information it is stated, that under the 35-years-old plantage of Robinia pseudoacacia L. the biochemical mineralization of organic phosphorus compounds passes considerably more actively than under Pinus pallasiana D.Don.
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Application of different compost doses in Pinova and Golden Delicious apple orchards
91-94Views:280Nowadays the success vegetable and fruit production are unimaginable without regular nutrient management. The animal husbandry is getting decrease, thereby there is the lack of organic fertilizer and it is suggested to find alternative nutrient supply methods. One of the ways to supply the required nutrients in an environmentally friendly way is the application of composts, which is less widespread so far, which is appropriate for the requirement of organic farming.
From the year of 2010 in each year, compost doses were applied in biological apple orchards in cooperation with the Institute of Horticultural Science. In the function of the years different changes were resulted by the compost treatments in the examined parameters in case of both apple species (Golden Delicious and Pinova). It was concluded that the dry matter, ash, total acids, sugar content and the vitamin C changed versus time and species. It is supposed that the effect of compost treatments is getting visible.
According to the data higher dry matter, total acids and sugar content, (in some cases) ash were measured in case of the apple species Pinova, while the measurements showed higher vitamin C content in case of the species Golden Delicious.
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Agri-Environmental Issues in the National Sustainable Development Strategy of the Member States of the Enlarged EU
346-353Views:190In preparation for the World Summit on Sustainable Development (Johannesburg), almost all EU member-states and some accession countries presented their national strategies for sustainable development (NSSD) during the summer of 2002. Along with other countries, Hungary is only now setting up this important document. Everywhere during the last decade, as in the EU countries, the intention was to involve the environmental problems into other political questions (agricultural, energy and transport policy, etc.). Today the strategy-builders have given up the sectoral view because of difficulties and setbacks, and use a view aiming the problem.
Agri-environmental issues appear as the elements of sustainability in the national strategies. For example, in accordance with the danger of global warming, the emission of green-house gasses of agriculture; in line with eco-efficiency, the production of renewable raw materials and energy sources originating from agriculture, and, in relation to changing lifestyle, food-safety and the future of biological agriculture will be disputed. With this study, I investigate the main characteristics of sustainable development strategies at both the EU level and at the level of the different member states from an agri-environmental point of view. -
Physical and chemical treatment of poultry feather from the slaughter-house
51-56Views:221The 15-20% of the by-products of meat – and poultry industry – that unsuitable for human consumption – contains keratin. The slaughter technology of poultry produces large amount of poultry feather with 50-70% moisture content. This means more million tons annually worldwide (Williams et al., 1991; Hegedűs et al., 1998). The keratin content of feather can be difficulty digested, so physical, chemical and/or biological pretreatment is needed in practice, which has to be set according to the utilization method.
Our applied treatments were based on biogas production, which is a possible utilization method. In the IFA (TULLN) Environmental Biotechnology Institute the feather was homogenized, and – according to the previous examinations – the most effective 1:2 feather-distilled water ratio or 1% NaOH-solution was used, and then treated with microwave (70, 130, 160 °C) during 1 hour time period. DM% and oDM% content was analyzed in the original samples, and the pH, Carbon-, Nitrogen-content in the output, too. Based on the received correlation coefficients (R) and related significance values (Sig.) I concluded, that the C-, N-content and the pH values weren’t influenced by any of the additives. The temperature
affected all three tested factors. The temperature showed a strong coherency with the N-content and the pH value when distilled water was used and weak-medium coherency with the Carboncontent. With NaOH-solution treatment the temperature gave strong coherency with the C- and N-content, as well as medium coherency with the pH. Our objective was to determine the method with effectively the pre-treating of poultry feather for biogas production or composting and to prepare of the treated samples for N and C analyzing. Our next aims will be the elaboration of the technological parameters of heat pre-treatment and microbial digestion of poultry feather for biogas production. -
New wonder of an old hormone: Hungarian sour cherry varieties as natural melatonin sources
65-72Views:408Agriculture has always been an important role in economy, food supplies, sustainability of society During our experimental work an effective extraction procedure has been developed for melatonin. Further chromatography was developed the quantitative and qualitative determination of melatonin. A compound that was equal to the standard was purified from the extract by preparative HPLC technique and the structure of the purified sample was confirmed by MALDI-TOF-MS and NMR analysis. Based on our measurements, harvested in the state of biological maturity Hungarian sour cherry cultivars contain high levels of melatonin.
Our results show that "VN4" variety has extremely high melatonin content, 9.893 ug g-1 and suggest that "VN4" which were selected from the "Bosnyák" varieties is melatonin accumulating. The average value of the melatonin content of Hungarian sour cherry cultivars is 2.319 ug g-1.
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Grain yield and quality of maize hybrids in different FAO maturity groups
126-131Views:216An improvement in the quality of maize grain by increasing the level of components responsible for its biological value is possible
by using genetic means. However, a change in the genotype, together with improving the nutrient properties of the grain, also has some
adverse consequences connected with a fall in yield and in resistance to diseases.
Field experiments were conducted during three years (2003, 2004and 2005) to evaluate environmental effects on grain yield and
quality responses of maize hybrids. Twenty one hybrids of various maturity groups (FAO 150-400) were planted to achieve an optimum
(60-70 000 plants per hectare) plant populations and grown under the medium-N (80 kg N ha-1) fertilization. Environmental conditions
significantly affected maize hybrid responses for grain yield, starch, oil and protein contents, and consequently, starch, oil and protein
yields per hectare. Hybrids of flint type, which have a short vegetation period, had high protein and oil content but the yield averages
were low due to the slower rate of starch incorporation. Hybrids of the dent type have a longer growing season and more intense
carbohydrate accumulation, but low protein and oil contents. In wet years there was a higher rate of starch accumulation, while dry
years are favorable for protein and oil accumulation. Positive correlation existed between starch content and grain yield and 1000-
weight as well as between oil content and volumetric weight among tested hybrids. Negatively correlation existed between grain oil and
starch content as well as between oil content and grain yield and 1000-weight. Thus, end-users that require high quality maize may need
to provide incentives to growers to off set the negative correlation of grain yield with oil and protein content. -
The examination of poultry feather digestility for biogas production
113-118Views:328The aim of this research was the elaboration of the technological parameters of biological digestion and biogas production from poultry feather produced in large quantities by slaughterhouses. Feather protein was digested by Bacillus licheniformis, keratin desintegrator bacteria. Investigations focused on the optimalization of parameters influencing poultry feather biodegradation. The optimal range of pH, temperature, feather size and bacillus:feather ratio were determined in the experiments as well as the analysis of relationship between the examined parameters. In order to be able to track the dinamics of the biodegradation, we determined the extintion level of the liquid phase of the biodegraded material in the different experimental treatments. The results showed that the rate of hydrolisis was significantly higher in the treatments with bacteria than in the treatments without it. The most extensive digestion were observed in case of 1:3 feather:water ratio. The highest intensity of feather digestion were detected in the treatment with 1% microbe ratio.
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Susceptibility of stem infected sweet corn hybrids to common smut disease
55-57Views:914The common smut of maize (corn smut, Ustilago maydis /DC/ Corda) can cause large economic losses in susceptible sweet corn hybrids as well. The protection against this pathogen is fundamentally based on prevention. Many methods to control corn smut have been recommended or evaluated, including crop rotation, sanitation, seed treatments, modification of fertility, and biological control. In spite of these frequently mentioned control strategies, the host resistance seems to be the only effective method to manage common smut in those areas where Ustilago maydis is prevalent.
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Application of AFLP-Method in Plant Sample Identification
207-213Views:351One possible method for the determination of DNA-polymorphism is the PCR-based AFLP (Amplified Fragment Length Polymorphism). This method had been succesfully introduced to the Department of Botany at University of Debrecen in 2000-2001 with the examination of hay saffron (Crocus sativus L.) and its allies. Hay saffron is grown as a spice for some thousand years producing the most expensive spice in the world. This plant is sterile, triploid reproduces only vegetatively with no fertile seeds. However its origin is unknown it exists only in cultivation and it is a mutated variety of another species or an artificial or natural hybrid. Usual methods for the systematic examination are restricted hence it seemed to be reasonable to apply molecular biological methods in its case. Results of this work include the introduction and many fold application of the method beside ensuring the consequences of science literature with determining the C. cartwrightianus to give the most similar genetical pattern to C. sativus.
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Values of Hungarian pigeon breeding
9-14Views:455Pigeon breeding has a history dating back several centuries in Hungary. It has internationally renowned and accepted results. Our aim was to estimate the range of values related to Hungarian pigeon breeding and draw attention to the importance of their conservation. We studied the biological values of native breeds and the appearance of pigeon breeding within the Hungarian culture. These include the use of the symbol, the relating beliefs and customs, language, gastronomy, architecture and object values, as well as the community-building effect. Based on our results we can say that this topic is worthy of further research.
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The potential of biological control on invasive weed species
73-75Views:327Sorghum halepense is one of the invasive species in Europe. This study was made to identify the morphology of fungi on invasive weed species samples on the roots of Sorghum halepense. The samples were collected in the region of Debrecen. The experiment was conducted under laboratory conditions to determine the microscopic form of fungi. The samples were put on PDA and for identification of fungi is based on the morphological characteristics of the features and colonies of conidia that were developed in Petri dishes.
The examination of the culture revealed that the fungus from the root of Sorghum halepense was Aspergillus niger. Pathogenicity and the relationship between the fungus and Sorghum halepense are still uncertain so in the future pathogenicity tests and re-isolations from plants are very important steps.
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The bioactive compounds of sour cherry (Prunus cerasus) with special regards to antioxidant activity and antioxidant density
83-87Views:594Evidence suggests that a diet high in fruits and vegetables may reduce the risk of chronic diseases, such as cardiovascular disease and cancer, and phytochemicals including phenolics, flavonoids and carotenoids from fruits and vegetables may play a key role in reducing chronic disease risk. Recent research has proven that sour cherry (Prunus cerasus L.) is a valuable natural source of some bioactive compounds important in human health preservation.
In our work, we identified the total antioxidant capacity and ”antioxidant density” of sour cherry varieties named ”Újfehértói fürtös”, ”Debreceni bõtermõ”, ”Kántorjánosi” and ”Érdi bõtermõ” and those of the ”Bosnyák” sour cherry clones. ”Antioxidant density” is a biological value indicator obtained in a synthetic way, which indicates the antioxidant capacity of the particular food, e. g. fruit and vegetable, per 1 Calorie. -
Element content analyses in the Institute for Food Sciences, Quality Assurance and Microbiology
203-207Views:385The role of chemical elements to ensure and promote our health is undisputed. Some of them are essential for plants, animals and human, others can cause diseases. The major source of mineral constituents is food, drinking water has a minor contribution to it, so the knowledge of elemental intake through food is crucial and needs continuous monitoring and by this way it promotes the food quality assurance and dietetics.
With the evolution of spectroscopic methods increasingly lower concentrations could be determined, so the elemental composition of a sample could be more precisely and fully described. Due to the results the gathered knowledge up to the present is supported and new observations can be done helping us to understand such complex systems as biological organisms are.
The quality of a food is determined by the full process of its production, consequently it starts with agricultural production so elemental-analysis usually cover the whole soil – plant – (animal) – food chain, by this way the „Fork-to-Farm” precept is true in elemental analysis field also.
The history of elemental analysis in the University of Debrecen, Centre for Agricultural and Applied Economic Sciences, Faculty of Agricultural and Food Sciences and Environmental Management, Institute of Food Processing, Quality Assurance and Microbiology goes back to 1980s when the so called Regional Measurement Central gave the background for research. The continuous deployment resulted in an obtain of an inductively coupled plasma atomic emission spectrometer (ICP-AES) in 1988, which extended the scope of examinations due to its excellent performance characteristics
compared to flame atom absorption (FAAS) and flame emission spectrometers (FES). The instrumental park retain up to date correlate to the developing analytical techniques due to acquiring a newer ICPAES in 1998 and an inductively coupled plasma mass spectrometer in 2004 – which sensitivity is three order of magnitude better compared to ICP-AES. The Institute supports the work with its own ICP-AES and ICP-MS since 2011. -
CO2 emission of the soil on barley stubble
95-102Views:291In the last decades the physical and biological status of the soils in Hungary significantly decreased. The degree and intensity of CO2-production of the soil is in close correlation to its structural status and organic matter content. In a complex soil tillage experiment at Karcag in situ measurements have been carried out since 2002 in order to determine the CO2-emission of the soil. Carbon-dioxide emission of the soil in the cases of conventional tillage and reduced cultivation system was analysed in a long-term cultivation experiment. The measurements were carried out after the harvest of the barley, thus root respiration was excluded. For the spatial delimitation of the measuring area a newly developed frame+bowl set was used. Based on measurements, significant differences between cultivation systems can be recognized due to the soil structure changes and its effects
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Studies of Expression of Peptaibol Synthetase of Trichoderma reesei
188-190Views:422Because of the potential importance of peptaibols in the biological control of plant diseases, a transgenic, a T. reesei strain carrying a tex1-promoter: goxA fusion plasmid was constructed for furthur studies. The peptaibol synthetase gene (which is highly similar to T. virens tex1) was identified in the genome sequence of T. reesei. A 900 bp 5’ upstream noncoding fragment, presumed to include the promoter region of tex1, was cloned into the pSJ3 plasmid (which contains the Aspergillus niger goxA gene encoding glucose oxidase). Finally, we transformed T. reesei with the tex1-promoter: goxA fusion containing pSJ3 plasmid.