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The effect of lead and copper heavy metal salts on soil microorganisms under laboratory circumstances
55-59Views:137he population dynamics of calcareous chernozem soils polluted with different concentrations of lead and copper heavy metal saline solutions was examined.
The experiment was carried out in the soil biological laboratory of the Institute of Agricultiral Chemistry and Soil Science at DE AGTC MÉK in 2012. For the determination of the concentration of the undiluted stock solutions we multiplied the smallest toxic concentration values of the MSZ 08-1721/1-86 Hungarian standard by forty. The intermediary concentrations of the treatments were produced with adequate dilution of the stock solutions until a dilution level equal to the values of the standard. The statistical evaluation of the data was performed with ANOVA (Analysis of Variance) including the determination of the standard deviation and significant difference. Investigating the effects of the different treatments on the soil microbes we established that both heavy metal saline solutions had a negative effect on the population dynamics of bacteria and microscopic fungi living in the soils. The negative effect of copper – as a potential toxic micro nutrient – turned out to be less strong than the negative effect of the toxic lead. According to our results the correction of the treatment levels is recommended in order to further tolerance examinations and the determination of the tolerance levels.
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The effect of biopreparations in pot experiment
45-49Views:129In pot experiment the effect of Amykor and Organic Green Gold bioproducts and their combinations with NPK fertilizer on some soil properties (chemical parameters) and on the biomass of testplant were studied. The experiment was set up in 2012 at the Institute of Agricultural Chemistry and Soil Science, in a three replications, in a random block design. The studied soil typein the pot experiment was humus sandy soil from Debrecen-Pallag with onion (Allium cepa) test plant. At the end of the experiment (after 4 week) in our laboratory the samples of soil and plant were determined. The nitrate-nitrogen, AL-soluble phosphorus and potassium content of soil, the weight of green onion leaves, the wet weight of bulb and root of onion and biomass of onion. The results of the study were the following: – The treatments influenced positively the nitrate-nitrogen, the AL-soluble phosphorus and potassium content of soil. – The most effective treatments were the artificial fertilization (NO3-N) and the NPK+ simple dose of Amykor (AL-P2O5 and Al-K2O). – The NPK fertilization and the NPK+OGG (sprinkle in every 10 days) combinations had significant positive effect on the weight of green onion leaves. – The biofertilization and the straw+biofertilizer combinations stimulated the AL-soluble potassium content of soil occasionally. – The OGG treatment (sprinkle in every 10 days) had significant effective impact on the wet weight of bulb and root of onion. – The biomass of onion was increased by the artificial fertilization and OGG (sprinkle in every 10 days) treatment.
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Microbiological preparations affecting the soil nutrient availability and growth of ryegrass in a pot experiment
49-53Views:136The effects of different bacterial fertilizers and their combinations with NPK fertilizer and wheat straw were investigated on some soil properties (chemical parameters) and on the biomass production of testplant. The applied quantities of the bacterial fertilizers were the double of the recommended dose. The experiment was set up in 2013 at the Institute of Agricultural Chemistry and Soil Science, in a three replications, in a random block design. Calcareous chernozem soil; originating from Debrecen (Látókép) was used with ryegrass (Lolium perenne L.) test plant. At the end of the experiment (after 8 week) the samples of soil and plants were determined for nitrate-nitrogen, ALsoluble phosphorus and potassium content of soil, the weight of green biomass of ryegrass per pot, the dry matter and moisture content of ryegrass. Straw treatment resulted better water and available nutrient content of soil in general. Inoculation however was not improving the biomass production over the fertilizer treatment. Interrelation with the recommended dose could be further studied.
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Impact of ammonium nitrate and Microbion UNC bacterial fertilizer on dry matter accumulation of ryegrass (Lolium perenne L.)
35-39Views:80Pot experiment was performed to investigate the effects of increasing NH4NO3 doses with or without Microbion UNC bacterial fertilizer
application on dry matter production of ryegrass (Lolium perenne L.). Experiment was set up on calcareous chernozem soil of Debrecen-Látókép and on humus sandy soil of Őrbottyán. The bi-factorial trials were arranged in a randomized complete block design with four replications. Grass was cut three times. Dry matter production was determined and the sum of biomass of cuts was calculated as cumulated dry weights. Analysis of variance was carried out on the data in order to provide a statistical comparison between the treatment means. The least significant difference (LSD5%) test was used to detect differences between means. On the basis of our results it can be concluded, that the dry weights of ryegrass cultivated on chernozem soil were higher than on sandy soil. With increasing nitrogen supply the dry matter production of grass significantly increased in both types of soils. In case of sandy soil the increasing effect was more expressed, but dry weights of this soil never reached the appropriate values of chernozem soil. Application of Microbion UNC had positive effect on dry matter production of ryegrass grown on both two types of soils but the effect was more expressed on chernozem soil. Finally it can be concluded that the increasing effect of NH4NO3 on biomass weights was more expressed in both types of soils, the biofertilizer application also increased the dry weights of plant in a small degree. -
The effect of different microbial preparations on some soil characteristics
83-86Views:97In pot experiment the effect of different microbial inoculants and their combinations with NPK fertilizer and wheat straw on some soil properties (physical, chemical, and microbiological parameters) were studied. The experiment was set up in 2011 at the Institute of Agricultural Chemistry and Soil Science, in a three replications, in a random block design. The studied soil type was calcareous chernozem soil from Debrecen (Látókép) with ryegrass (Lolium perenne, L.) test plant.
At the end of the experiment in our laboratory the nitrate-nitrogen content of soil, the AL-soluble phosphorus and potassium content of soil, the urease enzyme activity of soil, the total number of bacteria and the number of microscopical fungi were determined.
The results of the study were the following:
– The straw treatment and the straw + biofertilizer combinations influenced positively the nitrate content of soil.
– The NPK fertilization and the straw+bacterial fertilizer combinations had significant positive effect on the AL-soluble phosphorus content of the soil.
– The biofertilization and the straw+biofertilizer combinations stimulated the AL-soluble potassium content of soil occasionally.
– The total number of bacteria was influenced by the NPK fertilization, the bacterial fertilization and the straw+bacterial fertilizer combinations significantly.
– In case of the number of microscopic fungi caused in some cases significant changes the NPK+bacterial fertilizer and straw+bacterial fertilizer combinations.
– The soil urease enzyme activity was increased in all cases strongly especially by the straw+bacterial preparation combinations. -
The effect of different bacterial fertilizers on the AL-soluble P2O5 content of soil, and the biomass of the rye-grass (Lolium perenne, L)
93-98Views:186In pot experiment the effect of different bacterial fertilizers on some soil properties, and the amount of plant biomass were studied. The
experiment was set up in 2010 at the Department of Soil Science and Agricultural Chemistry, in a three replications in a random block design. The ryegrass (Lolium perenne, L.) was used as a test plant. The studied soil type was calcareous chernosem soil from Látókép. In our laboratory AL-soluble P2O5 content of soil, the phosphatase enzyme activityof soil, the dry weight of rye-grass, and the phosphorus content of rye-grass were determined.
The results of the study were the following:
– The bacterial fertilizers - by basic treatments NPK - had significant positive effect on the AL- soluble phosphorus content of the soil.
– The soil phosphatase enzyme activity was increased in all cases strongly by the microbial preparations used, the greatest impact was the Bactofil A bacterial fertilizer.
– The plant educed P values significantly increased by the effect of microbial products, in addition to the fund NPK. In this case, the EM-1 and Microbion UNC bacterial fertilizer were the effective.
– In case of the rye-grass biomass none of the bacterial preparations used caused any significant changes, either alone or when used them with straw treatment. -
Effects of long-term K fertilization and liming on the extractable and exchangeable K contents of a Haplic Phaeosem soil
141-145Views:30Effects of regular K fertilization and liming on the easily extractable K content of a Haplic phaeosem soil determined in 0.01 M CaCl2
and AL (traditional method in Hungary) were examined in the B1740 type of the National Uniformed Long-Term Fertilization Experiments
at Karcag.
Close correlation (r=0.95) was found between the 0.01 M CaCl2 and ammonium lactate - acetic acid (AL) extractable K contents of
soils.
K fertilization increased the amount of 0.01 M CaCl2 and AL extractable K significantly. Liming had different effects on the amounts of
K extracted by these two methods. Liming increased the amount of AL-K and decreased the amount of CaCl2-K. CaCl2 extractable K was in
close correlation with the relative amount of exchangeable K content of the soil (K%) and the agronomic K balance. The results of regression
analysis confirmed that the CaCl2-K characterized K% and the AL-K related to the absolute amount of exchangeable K.
On the basis of the presented results it can be stated that the 0.01 M CaCl2 is able to detect not just the increase of easily extractable K
caused by fertilization and liming but the changing of the rate of the relative amount of exchangeable K.