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Selenium speciation analysis of selenium-enriched food sprouts
23-28Views:183In this present study, we prepared selenium-enriched pea and wheat sprouts. During our research we aimed not only to measure the total selenium content of the sprouts but to identify different selenium species.
Scientifical researches show why the analytical examination of different selenium (Se) species is necessary: consumption of all kind of Se-species is useful for a person who suffers in selenium deficit, while there is significant difference between effects of different Se-species on person, in whose body the Se-level is just satisfactory. Biological availability, capitalization, accumulation, toxicity of Se-species are different, but the main difference was manifested in the anti-cancer effect of selenium.
During our research selenium was used in form of sodium selenite and sodium selenate, the concentration of the solutions used for germination was 10 mg dm-3. Control treatment meant germination in distilled water. Total selenium content of sprout samples was measured after microwave digestion by inductively coupled plasma mass spectrometry (ICP-MS). Different extraction solvents were applied during sample preparation in order to separate different Se-species (0.1 M and 0.2 M HCl or 10 mM citric acid buffer). We wanted the following question to be answered: Which extraction solvent resulted the best extraction efficiency? Selenium speciation analysis of sprout sample extracts was performed by high performance liquid chromatography with anion exchange column, detection of selenium species was performed by ICP-MS.
Evaluating our experimental results we have been found that significant amount of selenium of inorganic forms used during germination transformed into organic selenium compounds. There was difference between the amount of Se-species in pea and wheat sprouts and selenium uptake and repartition of selenium species were depended on Se-form used during germination. In addition the chromatogram analysis made us clear as well, that the citric acid solvent proved to be the most effective extraction solvent during sample preparation int he view of organic Se species.
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Investigating the above-ground biomass values of sweet potatoes (Ipomoea batatas)
35-39Views:97The role of sweet potato tubers in human nutrition is not new. The above-ground biomass of sweet potatoes is not used for nutritional purposes in most countries, but it has a high biological value. Therefore, the aim of the present study is to investigate the production of press fibre from above ground biomass by wet fractionation. Two sweet potato varieties (purple- and white-fleshed sweet potato) and two types of irrigation system were used: bubbling water flow system (BWS) and continuous water flow system (CWS). Glucan, xylan, arabinan were analysed by HPLC and elemental content was measured by ICP-OES. Our results show that the total carbohydrate content in the pressed fibre of the leaf blades (27.64–29.88 %w/w) is lower than in the stem with petiole (51.14–57.36 %w/w). No significant difference in glucan, xylan and arabinan content was observed in the leaf blade. In the stem with petiole, significant differences were observed for xylan and arabinan contents. For elemental content, generally higher values were measured in the leaf blade than in the stem with petiole. This information may be relevant for the selection of the appropriate variety and treatment, even for the production of functional food.
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Comparison of Reproductive Performance of the Roe Deer (Capreolus capreolus, L.) Among Different Regions
41-46Views:81The potential and actual number of offspring of roe deer and the difference between these figures (prenatal and postnatal loss) significantly vary in each population yearly. The objective of this study is to examine the potential and actual number of offspring, the number of losses, and to find a link between the most important biological characteristics of does (body weight – BW, condition – KFI) and the number of raised offspring on four territories on the Great Hungarian Plain.
Where the number of corpora lutea (CL) is the highest, there the losses are the highest as well, and the number of raised offspring is the lowest (region I.). Here, the rearing loss is double that of the weakest territory (region IV.). Rearing losses can be associated with the fenotype of does (BW, KFI) but environmental factors also have determinative importance. Where the number of twin-calving does was the highest, I found four times more does without a fawn than where the number of twin-calving does was the lowest. The nursing success was the best (the losses were lowest) in the region where the potential offspring (number of CL) was also the lowest, but the coverage of the habitat and the proportion of forests were largest. The food supply for the animals in autumn and winter are not enough, the structure of the habitat has to be improved as well, so that it might become adequate for game protection in extreme weather conditions.
The results have to be considered as preliminary ones. It is essential to continue and extend the research to increase the reliability of the results. -
Development alternatives of rural economy
187-191Views:100Environmental, natural, social and economic processes undergoing both in Europe and in the world predict such a 21st century that is characterised by increasing resource-crisis from both economic and ecological aspects. Therefore, it is very important for Hungary to see what happens to its natural resources, epecially to its agricultural land, water reserves as well as the biodiversity of the local unique flora and fauna. One of the most significant issues of the rural areas of Hungary is whether we can preserve the natural habitats and the various biodiversity of the related species, the favourable biological background of agriculture. In addition, whether we are able to provide high quality food for the country as well as for the broader reagion, whether we are able to produce energy from the resources available as well as to provide sufficient opportunities for the population to live and work. These can be considered as the most significant issuesof the coming decades which determine the strategy of the Hungarian rural economies in long term.
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Saccharomyces cerevisiae growth kinetics study dairy byproduct
169-172Views:167By guess, annual volume of milk whey is 185–190 million tons and this volume probably will increase next years. Whey has significant biochemical oxygen demand due to its high organic matter content so whey as sewage is one of the most pollutant by-products in the food industry. Apart from environmental pollution, benefit of several whey constituents for human health is another reason to utilize whey. Corn and potato, as well as the processing of milk in the food industry in large quantities of by-products generated by low cost, substantial quantities of starch and lactic acid, which are due to high biological oxygen demand are considered as hazardous waste. Some of them are destroyed sewage storage tanks, and those products are excellent substrates for the growth of microorganisms could be. The traditional nutrient solution optimization methods are solution and time-consuming and are not able to determine the real optimum because of the interaction of factors involved.
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Introduction of DNA-based Methods to Agriculture Through Molecular Taxonomic Examination of Poa Species
139-142Views:83biological methods, one among them is AFLP that is well applicable for taxonomic research. Bluegrass species, that are important components of meadow associations, thus their thorough knowledge is necessary in maintaining biodiversity, were examined with bringing this method to perfection.
Taxonomic relationship of the members of Poa pratensis aggregation is a controversial issue. Present study aimes to identify the members of this group, with a developed AFLP method through molecular taxonomic examination of Poa species in meadows nearby Debrecen, revealing their genetical distances. Species of the aggregation show a great genetic variability, but their genetic proximity approves the use of the term aggregation. Results established wider geographical investigation of three species of the aggregation. The distinctness of the species based on their morphological features was confirmed according to their genetical basis as well. The method overall turned out to be appropriate for the taxonomic research of bluegrass species like Poa pratensis and its aggregation. -
Methods for detention of lipid rancidity
117-120Views:198There are various methods available for measurement of lipid oxidation in foods.Changes in chemical, physical, or organoleptic properties of fats and oils during oxidation may be monitored to assess the extent of lipid oxidation. However, there is no uniform and standard method for detecting all oxidative changes in all food systems. The available methods to monitor lipid oxidation in foods and biological systems may be divided into two groups. The first group measures primary oxidative changes and the second determines secondary changes that occur in each system.
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The dynamics of biodiversity structure of soil microorganisms under the impact of biopreparations during potato growing season
67-74Views:141The use of biological preparations such as Phytotsid and Planryz contributes the increase of the general number of soil bacteria population by 13.0–36.1% in the case of potato variety Scarbnytsya and by 4.5–24.6% for potato variety Oberig compared with control. It also increases the number of saprophyte microflora, which compete with plant pathogens, micromycetes, and causes 1.2–1.8 times reduction in the number of soil fungi – Fusarium and Alternaria. During the application of Rovral Akvaflo the Shannon ecological index of species biodiversity is lower than during the biopreparation use. The decrease of species biodiversity was observed as well as strengthening the dominance of some species (dark pigmentation in fungi).