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  • Study of The Amount of Produced Animal Manure and Biogas Potential in a 5, 20 and 40 km Zone around Debrecen
    64-68
    Views:
    166

    The guiding principle for our research is that decentralized energy supply systems should be located near local consumers. One big, local market for energy from biogas plants in Hajdú-Bihar county is Debrecen. In the course of the investigation of biomass suitable for biogas production in the area of Debrecen, this study determines the quantity of biogas producible from animal manure. Municipalities around Debrecen were examined in circles with 40, 20 and 5 km radius. Livestock data of these settlements stem from the Agricultural Census in year 2000, actualized by the county trend of livestock changes. Manure quantity and biogas potential in these places are estimated with coefficients from the literature and our calculations. In the largest circle, about 1.4 million tons of manure is produced, from which round 94 million m3 biogas can be produced. Biomass produced in the settlements and producible biogas shows a remarkable trend of concentration, which supports our recommendation on the system of energy supply.

  • The marigold (Calendula officinalis L.) drug essential oil agents change under different fertilization settings in small plot trial
    57-60
    Views:
    411

    During our research we investigated the marigold's (Calendula officinalis L.) nutrient requirements with different fertilization setting in small-plot trial. We measured SPME (Solid phase microextraction) and GC-MS (gas chromatograph-mass spectrometer) we examined the effects of the different fertilization settings for the herb's main active ingredients of essential oil's percentage.

    Based on the results, it was concluded, the essential oil agents' percentage breakdowns significantly depending on the cropping technologies. Besides that it is possible, based on Pearson's correlation test the marigold essential oil agents relationship can also be a major factor.

  • Studies of the influences of different N fertilizers and Microbion UNC bacterial fertilizer on the nutrient content of soil
    134-140
    Views:
    320

    A field experiment was conducted to examine the effects of different nitrogen fertilizers in combination with bacterial fertilizer on
    nutrient uptake of horseradish and plant available nutrients of the soil. Three different N fertilizers, ammonium-nitrate, urea and calciumnitrate
    (116 kg ha-1 N) in combination with Microbion UNC bacterial fertilizer (2 kg ha-1) were applied as treatments in a randomized
    complete block design in three replications. In this paper we presented the results of soil measurements. The soil of the experimental area
    was chernozem with medium sufficiency level of N and P and poor level of K.
    Our main results:
    The amount of 0.01M CaCl2 soluble inorganic nitrogen fractions, NO3
    --N and NH4
    +-N and also the quantity of soluble organic-N were
    almost the same in the soil. N fertilizers significantly increased all the soluble N fractions. The amount of NO3
    --N increased to the greatest
    extent and the increase of organic N was the slightest. We measured the largest CaCl2 soluble NO3
    - -N and total-N contents in the plots
    treated with ammonium-nitrate, the largest NH4
    +-N in the plots treated with calcium-nitrate and the largest organic-N fraction in plots
    treated with urea.
    Bacterial inoculation also increased both soluble inorganic nitrogen forms and also total-N content of soil compared to the control. In
    the case of combined (artificial and bacterial fertilizer) treatments we measured lower NO3
    --N, organic-N and total-N compared to the
    values of plots having only nitrogen fertilizer treatments. On the contrary in the plots with combined treatments the CaCl2 soluble NH4
    +-N
    content of soil in more cases were higher than that of values with artificial fertilizer treatment.
    As a function of calcium-nitrate application increased AL-P2O5 and AL-K2O values were measured compared to control. Microbion
    UNC supplement of calcium nitrate yielded also increase in AL-P2O5 and AL-K2O values, till then supplement of ammonium-nitrate fertilizer
    yielded a decrease in these values compared to the control.
    All nitrogen fertilizers resulted in a significant decrease in AL-Mg content of soil compared to the control. Nevertheless bacterial
    fertilizer increased AL-Mg values in any cases.

  • Does drought stress always negatively affect the yield and quality of soybean in Hungary?
    37-40
    Views:
    450

    Globally-fluctuating climate imposed serious abiotic stresses on the agricultural sector, leading to noticeable, and sometimes disastrous, losses in yields and/or quality of crops; however, in certain cases, plants could survive stress with relatively low reductions, and sometimes even with some enhancements as a reaction to changed environment, especially in the case of mild stress. An experiment was conducted in 2017 and 2018 in Debrecen, Hungary to evaluate the mild drought stress influence on the yield and quality of three soybean cultivars. The results showed that both ES Pallador and Pedro cultivars could achieve more yield when subjected to mild drought conditions; however, protein concentration was enhanced in ES Pallador whereas slightly degraded in Pedro under drought. The cultivar Pannonia Kincse followed different trend; both yield and protein concentration were reduced under drought. Oil concentration of the three cultivars did not show significant changes; however, it always followed opposite trend to that of protein concentration. It could be concluded that both ES Pallador and Pedro are recommended to be grown under rain fed conditions in Debrecen, whereas Pannonia Kincse is recommended under irrigation conditions.

  • Fruit quality of sweet cherry cultivars in superintensive orchards
    75-81
    Views:
    799

    The fruit quality of 15 sweet cherry cultivars (’Canada Giant’, ’Celeste’, ’Chelan’, ’Ferrovia’, ’Germersdorfi Rigle’, ’Katalin’, ’Karina’, ’Kordia’, ’Linda’, ’Regina’, ’Sam’, ’Sandra Rose’, ’Sunburst’, ’Sylvia’ and ’Techlovan’) was studied under super-intensive growing conditions at Nagykutas. We measured the fruit diameter, fruit width, fruit height, stem length and stem weight, fruit and pit weight and the total dry matter content. There were large differences among the cultivars. These differences are due to the genetic characteristics of fruits because all other conditions were the same. For 11 cultivars, we collected fruit samples several times /2-4/. We examined on this cultivars all the above listed fruit quality parameters. When examining these samples, we have gained information how earlier or later than optimal harvest time influences fruit quality.

  • Allelopathic effect of invasive plants (Eriochloa villosa, Asclepias syriaca, Fallopia x bohemica, Solidago gigantea) on seed germination
    179-182
    Views:
    606

    The aim of this study was to determine the allelopathic potential of invasive species woolly cupgrass (Eriochloa villosa), common milkweed (Asclepias syriaca), bohemian knotweed (Fallopia x bohemica), and giant goldenrod (Solidago gigantea Ait.) on germination crop (Lepidium sativum L.). Experiments were conducted under laboratory conditions to determine effect of water extracts in petri dish bioassay. Water extracts from fresh biomass (leaves and stem) of invasive weeds in concentrations of 4 and 8 g/100 ml were investigated. All invasive plants showed allelopathic effect on germination. In giant goldenrod stem water extract experiment, allelopathic effect was less pronounced.

    The cress germination was greatly suppressed with the woolly cupgrass, common milkweed and the giant goldenrod. The experiment showed that the seed germination depended on the concentrations and the plant material used (leaves and stem).

  • Angle-count sampling method for estimating forest stand volume – a practical approach
    99-102
    Views:
    432

    Point sampling, which is also known as angle-count sampling (ACS), can be considered an efficient way of estimating the basal area and volume of forest stands. It is possible to use it in forest management: providing more accurate estimates (precision <10%) of  site and stand characteristics needed for management planning. 20 black locust (Robinina pseudoacacia L.) stands were selected at final cutting age to determine the regeneration criteria based on their total volume. It was verified that at P=5% there was no difference between the main volume values of stands indicated in the relevant forest plans as well as calculated by the ACS method.

     

  • Investigation of host-specificity of phytopathogenic fungi isolated from woody plants
    155-160
    Views:
    317

    Host-specificity is an important characteristic of fungal pathogens. Changing climate could create more appropriate environmental conditions for phytopathogens, thus formerly host-specify fungi could be able to colonize new hosts. Noxious plant pathogen fungi, which can infect several plant species are well-known worldwide. These genera may expand their range of hosts because of the appearance in new geographic areas due to climate change. This new exposure can result in serious problems in agriculture because of the lack of immunity. The susceptibility of apple tree was studied through testing pathogenicity in vitro with species isolated from walnut twigs and nuts, and identified by ITS sequences. Three of four tested species, Botryosphaeria dothidea, Diaporthe eres and Diplodia seriata colonized and necrotized the infected apple branches, while Juglanconis juglandina was not able to infect the twigs. Members of Botryosphaeriaceae were the most virulent, causing the largest lesions in the fastest way. This experiment draws attention to the threat of new host-pathogen connections, which can arise because of the favourable weather conditions and can spread between neighbouring cultures. 

  • Some population genetics parameters of the present Hungarian Hucul Horse population
    15-22
    Views:
    544

    We examined the Hungarian population of the Hucul horse breed, under genetic protection, based on population genetic indicators until the year 2014 included. The evaluation was performed using the Endog programme based on the following indicators: inbreeding coefficient, average relatedness, the maximum number of generations, the number of full generations traced and offspring number. Our findings were as follows: the average inbreeding coefficient of the total population was 5.99%, average relatedness was 11.82%, the maximum number of generations was, on the average, 16.04%, and the number of full generations traced with reference to the whole population was 4.15% on the average. 40% of the whole population (723 individuals) did not have any offspring; 42% (759 individuals) attained an offspring of 1 or 2, while 3.4% (88 individuals) had a surviving offspring of 3. The highest offspring number according to the national database (92) was attained by one stallion.

  • Isolation and identification of endophytic fungi connected to Grapevine Diseases, from the Tokaj wine region, Hungary
    61-66
    Views:
    406

    Grapevine Trunk Diseases (GTD) is one of the most important diseases in vineyards worldwide, which can be found in Hungarian vineyards as well. In Hungarian wine regions there is very little information about the occurrence of pathogens which cause GTD, in case of Tokaj wine region there is no knowledge about that, what kind of pathogens can be found in the vineyards.

    The objective of our research is to assess the situation and occurrence of GTD in Tokaj wine region in cooperation with local specialists, as well as identification of pathogens which were isolated from the diseased trunks by morphological and genetic basis.

    We were able to isolate endophytic fungi from all sampled grape trunk. The majority of them were determined as Diplodia seriata not only based on colony morphology, but also determined by rDNA sequences.

  • The Effect of Tillage Treatments on Soil Temperature at Planting and on Corn (Zea mays L.) Yield
    40-44
    Views:
    511

    The effect of soil temperature was evaluated on the yield of the Occitan corn hybrid at a depth of 5 cm. We examined this effect on the time required from planting to emergence for three average durations: five, ten and fifteen days, all calculated from the day of planting. Winter plowing (27 cm), spring plowing (23 cm), disc-till (12 cm) treatments and 120 kg N per hectare fertilizer were applied. As a result of our analysis, we determined the post planting optimum soil temperatures for various time periods. The average soil temperature for a time period of 15 days post planting is the most usable for determining actual yields, followed by ten days, with five days proved to be the least usable (winter plow R2 = 0.86, spring plow R2 = 0.87, disc-till R2 = 0.64).

  • The effect of cobalt-chloride on the production parameters and homogenity of barramundi (Lates calcarifer) larvae
    21-25
    Views:
    472

    Barramundi (Lates calcarifer) is a predatory fish species native in Southeast Asia and Australia. Based on the geothermal potentiality of Hungary the high market potential warm-water fish barramundi can be produced economically. Living nourishment organisms such as Artemia nauplii play an essential role in the larval rearing of barrramundi. However, zooplanktons in natural aquatic enviroments contain minerals in a higher concentration than the usually fed newly hatched Artemia. Therefore the goal of recent study was to investigate the effect of cobaltchloride on the larval growth and survival of barramundi when fed individually and combined with Zn and Mn supplemented Artemia.

    In our experiment a 24 hours period was used for the enrichment of newly hatched Artemia nauplii with cobalt chloride in itself, as well as in combination with zinc sulphate and manganese chloride. A total of 1900 barramundi larvae from 15–30 day post hatching were fed with supplemented Artemia in 9 groups of treatments in duplicate. The growth performance and elemental concentration of 40 larvae from each group was determined. All the groups produced significantly improved growth compared to the control (p<0.05). The lower concentration of individual Co supplementation resulted in a higher growth performance while the opposite dose relation occured when combined the Co with Mn. Cobalt had a significant negative effect on the Mn uptake of the larvae – significantly higher Mn accumulation compared to the control group was only observed when Mn was fed in itself (p<0.05). In case of Co-Zn-1 and Co-Zn-2 treatments significantly higher Zn concentration was measured than in the others (p<0.05).

  • The effect of immigration on some population genetic parameters of the Hungarian Hucul population
    91-96
    Views:
    406

    Population genetic indicators of the Hungarian Hucul population were calculated taking also into account the effect of imported horses.The birth year of the examined 3002 individuals ranged between 1871–2015. The last year involved in the examination was 2015, when the research was conducted on the stock under stud book control. The calculations on the population were made using the statistic indicators in the Endog programme, with and without the imported Polish horses. Figures were depicted using R software. Number of offspring of the Polish individuals was presented in tables; the distribution of the offspring per stud and per mare family, as well as the Nei’s genetic distance,were presented graphically.

  • Development of seed analyses by means of various matrix solutions and the MALDI-TOF MS technique
    53-57
    Views:
    808

    The earth's population is growing steadily, currently accounting for about 7.3 billion people. Population growth causes food demand to rise, approximately 36 million people die each year due to starvation or related diseases. One solution to this problem is the continuous examination and development of the agricultural economy. In this study, matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (MALDI -TOF MS) were used to analyse of sunflower, soybean and hemp. In order to analyse the protein of maize, this method has already been applied. However, for sunflower, soy and hemp, it is necessary to develop a sample preparation method. Choosing the optimal matrix solution for ionization the traget molecule is an essential part of developing the method. Our aim is to compare two different matrix solutions (α-HCCA, SA matrix), based on the properties (intensity, noise ratio, value of spectra) of the spectra.

  • The history and present of public drinking water supply and its regionalisation in Debrecen
    147-151
    Views:
    284

    The public water supply played a major role in the urbanization of Debrecen city, just like in the case of many other cities. We had plenty of water and so we wasted it. However nowadays we experience a considerable decrease in the level of groundwater which causes an increasing need of energy for pumping. Beside the above mentioned the ecological threat and the decreasing water quality are also major problems. This study attempts to draw attention to the possibilities and the future of the regional water supply, by showing the history of the water supply, the sanitation systems of Debrecen and their present day activities.

  • Using integrated remote sensing methods in the Nagyerdő Natura 2000 area
    19-24
    Views:
    506

    The more widely use of GIS, remote sensing technology provides appropriate data acquisition and data processing tools to build several national and international biodiversity monitoring system of environmental protection and natur conservation. The ChangeHabitats 2 is a similar international project, which uses airborne hyperspectral and airborne laser scanning (airborne LiDAR) sources beyond traditional data collection methods to build a monitoring system of Natura 2000 habitats. The goal of our research, on one hand, was to separate the most typical species of trees which can be found in the largest coverage in the research plots of Debreceni Nagyerdő Nature Reserve from field and airborne remote sensing data, use image classification that based on spectral and geometry (height) characteristics of the trees. On the other hand our goal was to evaluate the efficient use of the integration of mobilGIS, airborne hyperspectral and airborne LiDAR data collecting methods to complement or substitut of the traditional, field data collecting methods. We used ArcGIS 10.2 and Exelis 5.0 GIS software for data evaluation, in which the mosaicing, the selection of plots and the spectral image processing were carried out.

  • Review of the biology of plant psyllid (Cacopsylla pruni, Scopoli 1763), and its role in the spreading of European stone fruit yellows, ESFY-phytoplasma with Hungarian data
    25-33
    Views:
    602

    The European stone fruit yellows (ESFY) phytoplasma disease caused by pathogen ’Ca. Phytoplasma prunorum’ induces serious damages in cherry, sour cherry, peach, and apricot orchards mostly in Europe. Its known vector is the plum psyllid (Cacopsylla pruni). Many articles report on the biology (morphology, taxonomy, life cycle etc.) and the method of transmission of the pathogen by the vector, and the possibilities of their control. This paper reviews our knowledge about the vector, and summarises the results of an inland research carried out in a northeastern Hungarian apricot orchards. Our goal was to show some important data for the farmers or anyone who is interested in this disease and its vector. And give some known method that we can protect our orchards against them to prevent the appearance of the disease. As the psyllid that became infected with the pathogen can hold its infectionous capacity during their lifetime, it is very important to have enough knowledge about their lifecycle, that we can determine the right time and method to control them. We also have to know how to identify them; therefore, this paper lists several important data which can be helpful. The most important keys of identification are their wing color, which dark borwn in the apex and brown is in the remaining part of the forewing. The length of the antennae is also an important factor, since other genuse’s species have longer antennae than twice the width of the head. C. pruni has as long antennae as twice the width of the head. They return to Prunus species in early spring and we have to protect our orhards in this period against them. We have to use preparations with a knock down effect on them to prevent the inoculation of the pathogen into the trees in our orchards.

  • Random regression models for genetic evaluation of performance of the Hungarian show-jumping horse population
    87-91
    Views:
    415

    The aim of the study was to estimate genetic parameters for show-jumping competition performance using random regression model. Show-jumping competition results collected between 1996 and 2009 were analyzed. The database contained 272 951 starts of 8020 horses. Identity number and gender of the horse, rider, competition date, the level of the competition and placing were recorded in the database. Competition levels were categorized into five groups. Weighted – competition level used – square root transformed placing was used to measure performance of horses. The random regression model included fixed effects for gender, year and place of competition, and random effects for rider, animal and permanent environment.

    Later performance of show-jumping horses measured with weighted square root ranks is less influenced by rider and permanent environmental effects than performance at the beginning of a horse’s sporting career. Heritability increased continuously from 6.3 years of age (2296 age in days), values were in the range of 0.07 and 0.37. Higher heritability was found in later ages. Weak genetic and phenotypic correlation was found between the early 4–5–6 years of age and older (7, 8, 8+) age classes. From 8.5 years of age (3132 days old) there were strong genetic and phenotypic correlations between neighboring age groups. For the same age classes moderate and strong genetic and phenotypic correlation was found. Genetic correlation between 13.5 years of age and older horses was very strong.

  • Testing laboratory parameters of compost tea
    31-36
    Views:
    989

    During the industrial production of broiler chicken, a large amount of manure is produced, of which easily contained nitrogen content (without pre-treatment) is released into the atmosphere as an air pollutant. In our experiments, we aimed to prepare compost tea, also known as water extract of compost, from pre-treated poultry manure in order to create a product can be utilized as liquid nutrient supply. The poultry manure source was the Baromfi-Coop Ltd. located in Nyírjákó, Hungary, where it was treated by composting. As a result of this pre-treatment of the poultry manure, its nutrient parameters improve and nitrogen is present in a form that is better utilized for plants. Furthermore, this product is suitable for further utilization and also can be the base material for a brand-new product. For this reason the effects of compost/water ratio, incubation time, low oxygen level, and extraction time on the parameters of the resulted product were studied in the frame of developing new soil-life enhancing microbial product, so-called compost tea.

    Chemical parameters of the compost used as base material strongly determined the properties of the resulted compost tea, especially the ratio of the various nitrogen forms, their concentration and the salt content. It was found that adding water at a higher rate that means 1/40 and 1/50 mixing ratios results in more cost-effective production. In the experiment the compost tea were held under oxygen-poor conditions, therefore pH of the extractions decreased, which influenced the quality and quantity of their nutrient content.

  • Assessment of electrical conductivity and germinability of groundnut genotype seeds
    5-10
    Views:
    592

    Seed quality affects crop establishment and productivity. In addition, the use of good-quality seed is an essential prerequisite for sustainable crop production including groundnuts. Assessing germinability and electrical conductivity provides early evidence of the production potential of a given crop variety or genotype. Therefore, this study assessed the germinability and electrical conductivity of seeds of three groundnut genotypes. A laboratory experiment arranged in a Completely Randomized Design (CRD), replicated three times, was conducted at the Faculty of Agriculture, Kyambogo University, in 2020. Seeds of Igola, Serenut 1, and Serenut 2 groundnut varieties were tested, and data was collected on germination percentage and electrical conductivity. Analysis of variance (ANOVA) was performed using GenStat and means were separated using the least significant difference test at a 5% probability level. Germination percentage and electrical conductivity significantly (p<0.05) differed among the groundnut varieties, with Igola recording the highest germination percentage, followed by Serenut 1, and the lowest was in Serenut 2. The highest electrical conductivity was recorded in Serenut 1 and the lowest in Igola. Since Igola had one of the lowest electrical conductivity and the highest germination percentage, it was concluded that Igola genotype retained higher quality attributes.

  • Mitigation of the effect of secondary salinization by micro soil conditioning
    115-119
    Views:
    427

    This research has the general goal to meet the customization of agriculture in small scale farming. We are developing a technique using micro doses of soil conditioners and organic material applied in the root zone of vegetable crops. We expected to change the physical and chemical properties of the affected soil, which has been irrigated with salty water. Two different soil conditioners were tested. A lysimeter experiment including 8 simple drainage lysimeters was set up in the Research Institute of Karcag IAREF University of Debrecen in 2017. The main goal was to study the effect of different soil conditioners on the soil endangered by secondary salinization induced by irrigation with saline water. In order to compare the difference between the treatments, we collected soil samples, water samples, and determined the yields. Chili pepper (Capsicum annuum) was used as an indicator crop during one specific agricultural season. The technique called micro soil conditioning is rational because several reasons. The roles of the technique are various, for example it can serve as a source of carbon or a container for soil amendments and can minimize evaporation. We found this technique not to interfere with the chemical reaction or the interaction with the plants. However, the micro doses of soil amendments had the role to minimize the risk of soil degradation and do not significantly influence soil respiration. In addition, by improving soil properties, soil conditioning increases the leaching of the excess of salts from the root zone. In fact, this technique can decrease the cost of the inputs and improves the production of vegetables, and at the same time mitigates the effect of secondary salinization.

  • Evaluation of the blood product characteristics of meat meal and hemoglobin with non-invasive methods in the VISNIR wavelength
    49-56
    Views:
    630

     

    The separate collection of poultry slaughterhouse trimmings and blood is partially solved in Hungary. Only properly prepared animal by-products, protein meals can be utilized as animal feed additive. However, different protein meals are appropriate for feeding different animal species. That is the reason why it is important to avoid accidental cross contamination of the products. Meat and blood meal produced on the same technological line, therefore mixing of the products can happen in various proportions during the shift of production.

     

    Thus the aim of this study is to develop a spectral method which will allow to estimate the ratio of meat and blood protein meal in the final product. During the test the products were mixed in different proportions and were examined by the spectral method. Measurements were conducted with AvaSpec 2048 spectrometer in visible (VIS) and in near infrared (NIR) wavelength range (400–1000 nm) to define the spectral differentiation of the different meal products. Significant difference can be detected in spectral reflectance between the meat and blood product in the VIS-NIR range. The blood product has a characteristic spectral property: in the range of 600 and 735 nm reflectance values are increasing following a sigmoid curve. This property is not observed in the case of meat meal: close to linear rising is detected. Effective protein rate and purity detection could be made by Blood Product Sensitive Mixing Index (BPSMI – R930/R600), and by the calculation of inflection point in 600–735 nm.

     

  • The Role of the Digital Terrain Models in the Assessment of Surplus Water Risk at the Szolnok-Túri Plain
    47-51
    Views:
    374

    The environmental factors to which surplus water can be assigned (topography, soil, groundwater, vegetation etc.) can be subject to special analysis and the randomness of the occurrences can be limited. The results of these procedures are surplus water risk maps of the areas, which can be utilised in land use planning. The risk map of the research site was created with overlaying digital category maps of the determining factors (hydraulic conductivity, convexity, critical probability of ground water level and land use).

  • Effect of the dry and rainy weather on the Idared and Golden Reinders apple varieties fruit quality
    107-110
    Views:
    415

    Weather conditions have an important role in fruit production. In the last few decades, this role is increased and basically determines the
    fruit quality and quantity. Despite of this statement, there is but very few information about impacts caused by weather anomalies in
    Hungarian orchards. Regarding this, the relation between the external, and internal values of the Idared and Golden Reinders apple varieties,
    the changing of the temperature and precipitation in a dry year (2009) as well as in a rainy year (2010) were investigated. The examined
    apple varieties are grown at the same training system (rootstock, spacing, training, pruning system). The average weight, average diameter,
    acid content and soluble solids were higher in 2009 than in 2010, although the precipitation was higher in 2010. Due to the many rainy days
    the intensity of sunshine and the number of sunny days were less. To produce better quality it is very important the amount of the sunshine
    and heat, the optimal temperature and the precipitation rate.

  • Adopt the tobacco plastid transformation technics in Hungary
    73-76
    Views:
    241

    Under the GENOMNANOTECH Debrecen Regionális Egyetemi Tudásközpont (GND RET) research program in summer 2007 in UD Centre for Agricultural Sciences and Engineering Faculty of Agricultural Science Institute of Horticulture experiments were launched to transform tobacco plant by plastid transformation technics.
    Our aim was to adopt first in Hungary the tobacco plastid transformation technics, which were used in Waksman Istitute Rutgers, The state University of New Jersey (USA) leading with Prof Dr. Pál Maliga. Scientists won scholarships learn and use this technics in the University of Debrecen.