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The effects of fertilization on the protein related properties of winter wheat
67-69Views:137The yield and quality of wheat are mainly determined by the plant production system, thus we studied the effect of mineral fertilization.
The field trials were set up in 1983 at the Látókép Research Institute of the University of Debrecen. We examined effect of different Nfertilizer doses (60 kg ha-1 N/P/K, 120 kg ha-1 N/P/K) on Lupus, Mv Toldi and GK Csillag's protein properties in 2012. During the tests, three quality parameters were determined: wet gluten content (%), wet gluten spread (mm/h) and gluten index (%). In the experiment the effect of different doses of N-fertilizers significantly influenced by the wet gluten content and gluten index of Lupus.
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The effect of NPK treatments on the Cu and Fe content of winter wheat (Triticum aestivum L.)
31-34Views:215In this study the effect of N, P and K nutrients on the Cu and Fe content of winter wheat (Triticum aestivum L.) grains was investigated in a long-term fertilization experiment set up in Nagyhörcsök. Samples were also harvested from four experimental stations of the Hungarian national long-term fertilization trials. These are the following: Bicsérd, Iregszemcse, Karcag, and Putnok. Plant samples were collected in 2005 which was very wet. Our results from Nagyhörcsök were compared with the Cu and Fe content of samples which were harvested from control plots of other experimental stations. The Cu and Fe content of grain samples were measured using inductively coupled plasma mass spectrometer (ICP-MS) followed by digestion with HNO3-H2O2 solution. All data were subjected to ANOVA, and when significant differences (P<0.05) were detected, Duncan’s test was performed to allow separation of means.
The main conclusions are as follows: Cu and Fe content of wheat grains was higher and higher in every NPK treatments. Samples were harvested from the control plots of Iregszemcse and Bicsérd have higher Cu content than the treated samples from Nagyhörcsök.
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The effect of short term storage on different winter wheat varieties rheological properties
83-86Views:249The aim of storage after harvest is to protect the quality of wheat, because after-ripening occurs in the first 5–6 weeks. During this time it very important to make the optimal storage conditions.
We have carried out storage experiment with wheat samples from Látókép Research Farm of the University of Debrecen. We analyzed the rheological parameters of Lupus and GK Csillag varieties from the crop year 2011/2012. The experiment period was between July and August 2012 (24. 07. 2012., 31. 07. 2012., 21. 08. 2012.).We determined the rheological parameters (water absorption, dough stability time and valorigraph quality number) of Lupus and GK Csillag during short term storage. Our results showed that after-ripening increased the baking quality of Lupus and GK Csillag during storage.
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Variations in major quality parameters of forage and medium quality winter wheat varieties in storage
249-254Views:146We analysed five parameters (moisture-, protein content, Hagberg’s falling number, wet gluten content and alveographic W (10-4 J) values) and the microbiological changes of four forage and milling III. quality winter wheat varieties (Magor, Hunor, Róna and Kondor) during storage, to determine the tendency, type and volume of the of the change of this five qualitative parameters during storage.
We found that the examined winter wheat varieties retained their moisture, protein content and their Hagberg’s falling number, they did not change during storage.
A slight growth could be experienced in the values of wet gluten content for all the four winter wheat varieties in terms of the duration of storage (129 days). This result proved the theory of after-ripening, when gluten percentage improves qualitatively and quantitatively as well. The value of the quantitative growth was about 10% for all the four winter wheat varieties.
We placed a special emphasis on measuring the alveographic W (10-4 J) values during storage. All the four winter wheat varieties showed decreasing values of about 20-40%.
Microbiological examinations on the four winter wheat varieties showed that mould, mould flora and total germ count remained balanced with some slight variations and they did not change in terms of time under optimal storage conditions. -
Investigation of genetic diversity in irradiated maize lines and its relation to hybrid performance
20-26Views:227Knowledge of genetic diversity among available parental lines is fundamental for successful hybrid maize breeding. The aims of this study were to estimate (1) genetic similarity (GS) and genetic distance (GD) (based on Jaccard index) in four maize inbreed lines; (2) to classify the lines according to their GD and GS; (3) to determine hybrid performance based on GD and heterosis for yield ability in 4x4 full diallel system. We used morphological description and AFLP (amplified fragment length polymorphisms) for estimation genetic polymorphism in four maize inbred lines. We estimated the applicability of genetic similarity in SC and reciproc hybrids for prediction of their performance.
Three primer combinations were used to obtain AFLP markers, producing 207 bands, 70 of whit were polimorphic. The dendogram based on genetic similarities (GS) and genetic distance (GD) and morphological description separated four inbred lines into well-defined groups. Morphological description just with AFLP analysis showed reliable results. In view of genetic distance, the UDL 1 line and their linear and reciprocal crosses showed significant heterosis effect, which was confirmed by heterosis calculation based on grain yield. -
Connection of protein and amino acid content of forage and medium quality winter wheat varieties
101-107Views:209We analysed the crude protein content, amino acid content, amino acid composition of four forage and milling III. quality winter wheat varieties (Magor, Hunor, Róna and Kondor) from their samples from five following years (2003, 2004, 2005, 2006, 2007). We found that quantity of essential and non-essential amino acids rose with increase in crude protein content. On examination of protein amino acid composition in relation to crude protein content we found that the crude protein content increased the quantities of the non-essential amino acids also rose, while those of the essential amino acids decreased as the lysine, the limiting amino acid of wheat. We also established that, as crude protein content increased, the biological value of the protein decreased.
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Connection of crude protein content and amino acid content of forage and medium quality winter wheat varieties in the relation of quantity and quality
73-79Views:206We analysed the crude protein content, amino acid content, amino acid composition of four forage and milling III. quality winter wheat varieties (Magor, Hunor, Róna and Kondor) from their samples from five following years (2003, 2004, 2005, 2006, 2007). We found that quantity of essential and non-essential amino acids rose with increase in crude protein content. On examination of protein amino acid composition in relation to crude protein content we found that the crude protein content increased the quantities of the non-essential amino acids also rose, while those of the essential amino acids decreased as the lysine, the limiting amino acid of wheat. We also established that, as crude protein content increased, the biological value of the protein decreased.
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Model Experiment Analysis of the Antioxidant Effect of Conjugated Linoleic Acid
15-18Views:183Corn milled like flour was crumbled with 5% butter containing a high level of conjugated linoleic acid, then kept exposed to air on an aluminium tray at a layer of 1 cm thickness. Its acid number, peroxide number and fatty acid composition were measured weekly. It was established that during a 24 week long period, there was very little change in the composition of fatty acids, but after this, in parallel with the increasing acid number and peroxide number, the amount of unsaturated fatty acids decreased, while those values for saturated fatty acids did not change considerably. With these investigations, the authors proved the antioxidant effect of conjugated linoleic acid.
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Influence of Foliar Treatments on the yield and quality of Sugar Beet
119-123Views:131The yield and quality of the sugar beet are mainly determined by the plant production system, thus we studied the effect of mineral fertilization. Our field trials were carried out in 2005 in Hajdúböszörmény, at two sites. We studied the effect of sulphur (Cosavet DF), copper (Kelcare Cu) and two foliar nutrients with high active agents. Treatments were replicated four times.
We took root samples at 4 week intervals, starting in August. The quality of root (sucrose, potassium, sodium and alfa-amino N content) was determined from filtrated beet broth, by an automatic beet laboratory system, called VENEMA.
We found that the crop and the sugar yield were significantly influenced by the foliar treatments -
Study of traditional folk foods from the point of view of nutrition in Hajdú-Bihar County
47-51Views:141Healthy nutrition is one of the major topics of our days. The occurrence of the so called “civilization diseases” is increasing continuously as a result of the changes in our nutritional habits and life-style. It was thought that folk nutrition at the beginning of the 19. Century had several beneficial effects on health. For this reason it was decided to study the food and nutritional habits of the population of Hajdu-Bihar County around the 1930s on the
basis of literary sources to see how well they do match with modern requirements of healthy nutrition. -
Research on rheological characteristics of winter wheat varieties
266-272Views:188The gluten examination test is dominant in Hungary, comparing it to the qualification system of other countries. The determination of alveographic parameters is a basic criterion of winter wheat specifications in some western and southern European countries.
In the Hungarian and foreign winter wheat qualification systems, in the standards, there is no limit for extensographical parameters. Customers dictate the limits and make their claims as to the specifications.
We analyzed the alveographic and extensographical parameters of 19 winter wheat varieties grown by the Cereal Research Non-Profit Company in Hungary, and we made a comparison between the results in the challenges of the European Union expectations.
Examining the information of alveographic and extensographical values, we found that the experimental varieties provide high base to flour types suitable for baking bread and baker’s ware. The GK Élet, GK Petur, GK Memento, GK Csillag, GK Kapos and GK Marcel varieties can meet the market of paste flour needs, too.
In the variety series, the GK Kalász represents the highest values and the GK Garaboly shows the lowest parameters regarding the alveographic W and the resistance to extension.
On the basis of relation, we can establish that we can estimate the following little known and used quality indexes: the extensographical resistance to extension with extensographical energy and the alveographic W value, the extensographical energy with the alveographic W value and the alveographic P and L values with the other alveographic parameters. -
Quality analysis of the near-infrared spectra of wheat samples
97-100Views:151The near infrared spectroscopy is widely used in the different industries as a rapid, non-invasive analitical tool. It is suitable for identification, qualification and quantitative analysis as well. As this technique is indirect, to make accurate calibration equations we need a proper sample population. Before the quantitaive analysis, developing calibiration modells we have to collect and examine the spectra. In our study we examined wheat samples with known origins to find if there is any effect of the growing area on the NIR spectra.
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The Effect of Grazing Intensities on Magnesium Contents
8-13Views:246Research was carried out on two areas of grassland in Hortobágy National Park, Hungary. Two herds of Hungarian Grey Cattle were kept in free range grazing and the effects of grazing pressure on the magnesium content of soil and ryegrass (Lolium perenne L) were determined.
Changes of plant available and total soil magnesium content under different grazing intensities did not show any evident tendency on the investigated grasslands. Different amounts of cattle faeces, urine and trampling had no effect on the magnesium concentration of ryegrass. We conclude that the magnesium content of ryegrass on both grassland sites as moderate grazing and overgrazing matches the requirements of cattle. Symptoms of magnesium deficiency of cattle will likely not appear. -
Effect of mineral fertilizers with different sulphur content on the yield of winter wheat in a small plot experiment
97-100Views:153We can find more and more references on the importance of sulphur and sulphur fertilization, mainly in the case of most important field crops, as cereals, maize, and the oilseeds. The traditional sulphur sources continuously lost their importance by the stringent air pollution orders and nowadays they are not able to meet the demands of plants. Besides, the application of superphosphate, as phosphorus mineral fertilizer, decreased in several countries. Sulphur fertilization is required in most cases to reach the required yields and quality parameters. The lack of
sulphur causes decreased nitrogen utilization and yield by cereals as winter wheat, moreover, results less favourable baking parameters.
We have examined the N and S content of different parts of winter wheat plants in a small plot experiment at the University of Debrecen, Centre of Agricultural Sciences, Látókép Experimental Station. We have also studied the effect of different sulphur fertilization forms and doses on the baking quality parameters and yield. Our aim was to contribute to the development of yield and quality improving sulphur fertilization methods, adaptable by practice.
We have measured the lowest yield in the case of one of the control plots, and 100 kg/ha nitrogen, 80 kg/ha potassium and 70 kg/ha phosphorus nutrient supply resulted the lowest yield (8.7 kg/plot). The maximum yield, 10.2 kg/plot was also experienced on superphosphate fertilized plot by the application of 140 kg/ha phosphorus dose. Application the first dose of both Biofert and FitoHorm 32 S leaf fertilizers raised the yield from
9.1 to 9.9 kg/plot. ANOVA proved that Biofert and FitoHorm 32 S had no significant effect on the amount of yield, the increase is only a tendency. -
Foliar treatments of Sugar beet
55-59Views:122The yield and quality of the sugar beet are mainly determined by the plant production system, thus we studied the effect of mineral fertilization. Our field trials were carried out in 2005 and 2006 in Hajdúböszörmény, at two sites. We studied the effect of sulphur (Cosavet DF), copper (Kelcare Cu) and two foliar nutrients with high active agents. Treatments were replicated four times.
We took root and leaf samples at 4 week intervals, starting in August. The quality of root (sucrose, potassium, sodium and alfaamino N content) was determined from filtrated beet broth, by an automatic beet laboratory system, called VENEMA. Leaf samples were measured with ICP-OES.
We found that the crop and the sugar yield were significantly influenced by the foliar treatments. -
Molybdenum - accumulation dynamics of cereals on calcareous chernozem soil
81-85Views:139This work is about the molybdenum-accumulation of cereals analyzing soil and plant samples from a field experiment set in
Nagyhörcsök by Kádár et al. in 1991.
In this long-term field experiment different levels of soil contamination conditions are simulated. Soil and plant samples were collected
from the experiment station to study the behaviour of molybdenum.
In this report results of maize, winter wheat, winter barley and soil analysis are presented. The conclusions are as follows:
– Analysing soil samples from 1991 we have found that roughly half of the molybdenum dose applied is in the form of NH4-acetate+EDTA soluble
– Comparing element content of grain and leaf samples we have experienced that molybdenum accumulation is more considerable in the vegetative plant parts
– Winter wheat accumulated less molybdenum then maize in its vegetative parts. Comparing molybdenum content of winter wheat to winter barley we found that the concentration of the element in wheat was lower by half than in the winter barley. It seemed that molybdenum accumulated to the least degree in winter wheat. -
Investigations on Mud on Heavy Metal Contaminated Flood-Plain of Tisza
96-101Views:136At the beginning of the year 2000 subsequently to a mine accident high heavy metal content mud entered the catchment area of the Tisza and was transported through the whole Hungarian section of the river. The majority of the heavy metals had been bounded to the floating sediment that was deposited on the flood-plain soil during flood forming a new, 5-10 cm thick layer. In the mud samples collected after the flood there was a clearly visible dark grey layer with significantly higher heavy metal content that was formed by the pollution wave and it was sorruonded by a light layer. The upper layer of flood-plain soils are formed from this mud layer during the soil development process, so the amount of Lakanen-Erviö soluble heavy metals that correlate with bioavailable heavy metal content was examined as well. In this case only the lead content was significantly higher in the dark layer.
New mud samples were collected after the 2001 flood. Separate layers could not have been identified, their colour was similar to those of the previous year’s light layers’. Comparing to this light layer the total Zn and Lakanen-Erivö soluble metal content was significantly lower in the mud samples of the year 2001. While the proportions of total and Lakanen-Erviö soluble metal concentrations were equal in both of the layers regarding the elements, these ratios have significantly changed next year regarding Pb and Zn: the amount of Lakanen-Erviö soluble metals considerably decreased.
As a result of sequential extraction the heavy metal content was rather low in the water soluble and exchangeable and NaOH-soluble fractions, so heavy metals found in the mud could be released in greater amount only in case of a heavy acidification. -
Analysis methods of quality and traceability for honey
25-32Views:208Honey is our essential food since ancient times. Thanks to the excellent dietetic and medical features, it has an important function in our nourishment. In Hungary about 25.000 tons of honey are produced each year. Most of the produce, about 80% is exported to different countries of the world. Hungarian types of honey have excellent quality. Through exporting, the prestige and demand of Hungarian honey can be increased. In recent years the adulteration of honey caused many damages to producers and traders. The adulteration of honey is not easy to prove. Thereinafter, we represent several methods, such as oligosaccharide analysis or 13C/12C isotopic ratio analysis, which can identify the artificial substances of honey (for example: corn syrup, high fructose corn syrup) or can help to determine the ratio by which original honey can be identified.
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Application of the SDS-PAGE method for the characterisation of winter wheat flour quality
112-118Views:133The principle, development and importance of the SDS-PAGE method are presented in this article. The SDS-PAGE method has become one of the basic methods of molecular biological research, because it is widely applicable and its sensitivity is excellent in the separation of wheat storage proteins.
We have shown the application of this method with a concrete example. It was also tested whether, it was possible to obtain a better baking quality product from a large amount of poor quality less valuable wheat by fractioning the flour according to particle sizes after grinding. We studied the rheological properties of flours with different particle size fractions from the original flour. The baking quality of the original flour was B2. The 125-90 and 90-63 μm fractions have significantly better baking quality (B1) than the original flour. The protein contents of these flour fractions were also significantly higher than the protein content of the original flour. We had a question: what has influenced the baking quality: the protein content or other factors? We searched for an explanation on these results in the protein composition of the flour samples. We studied the distribution of glutenin-fractions by SDS-PAGE method and evaluated them. We found with correlation analysis that the amount of LMW-Glutenin D-group (52-60 kDa) is in a strong, negative correlation to the baking quality (r = – 0.855*). Therefore, the baking quality of flour samples was influenced by this glutenin fraction. -
Antioxidant activity and total flavonoid content of honeys
25-28Views:173The influence of honey on human health has been known since ancient times. Different components, like vitamins, amino-acids, enzymes, and the so called antioxidant activity of the honey play role in this effect, and also in the excellent quality. The aim of present study was to determine the total antioxidant activity and the flavonoid content of some honey samples. These two parameters are widely studied, and a lot of data can be used to compare and analyse. On the basis of them we can state that our results are quite similar than those we can find in literature. Characteristic feature of the examined honey samples is, that the antioxidant activity increased with the darkening of the colour. The lowest antioxidant activity and flavonoid content was produced by the acacia honeys with greenish shade of colour. The highest values was found in the very dark honeys (forest, sage, golden rod). The only exception was the coriander honey, where despite of its lighter colour, very high antioxidant activity was
measured. -
The effect of climatic change on the rheological properties of winter wheat doughs
96-100Views:123In present paper we have examined the effect of climatic change on the extensigraph characteristics of wheat-flours. The baking quality
of winter wheat is largely determined by cultivar, but it can be influenced by weather conditions during growing period. Flours were from 5
cultivars grown at one location in three cropping years. We have found that the extensigraph properties of dough are affected by the weather
conditions, nevertheless, different cultivars distinctly react to the increase of temperature and decrease of precipitation. In generally, the
higher average day temperature and lower precipitation level is favourable to produce winter wheat flour with better quality -
The effect of different fertilizer treatments on the sulphur and protein content of wheat
73-76Views:154In this study the effect of N,P and K nutrients on the S and protein content of wheat grains was investigated in a long-term fertilization experiment set up in Nagyhörcsök. The calcareous chernozem soil having the following characteristics: pH (KCl): 7.3, CaCO3: 4.27%, humus: 3.45%, Al-soluble P2O5 and K2O: 60–80 and 180–200, KCl- Mg: 150–180, KCl+EDTA-soluble Mn-, Cu- and Zn-content: 80–150, 2–3 and 1–2 mg kg-1. The experiment had a split-split-plot design with 40 treatments in 4 replications. Plant samples were collected from 2002 and 2004. 2002 was a drought year while 2004 was very wet.
The main conclusions are as follows:
– The sulphur and protein content were than the control higher in every NPK treatments.
– The sulphur and protein content of the wheat grains were higher in 2004 that had a lot of rain than in 2002 that had drouht. -
The Role of Cultivation Systems in Quality Sugar Beet Production
134-138Views:131Producing sugar beet, as it is a demanding field crop, has contributed to the raising standard of plant production. It has an outstanding place among the plants that are cultivated in the intensive plant production system. Rentability of sugar manufacture is determined by the stability of yield and the quality (saccharose content) of sugar beet. In this way, the fundamental interest both of the producers and the processing industry is high yield and quality, year by year. The yield and the quality of the sugar beet are mainly determined by the plant production system, so we studied the effect of fertilization, irrigation and plant protection.
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Comparison of quality parameters of producers' and commercial honeys
31-39Views:255Honey is our essential food since ancient times. In Hungary about 25 000 tons of honey are produced each year, and most of the product (80%) is exported. Hungarian honeys have excellent quality but because of scandals of honey adulteration the interest and recognition may decrease. Therefore we must elaborate a method with which the establishment of adulteration is simple and cheap. In this study we concentrate on simple quality parameters which inspection is not elaborate. These parameters for example sugar- and moisture contains, proline content, electrical
conductivity, element content, pH, HMF content, degree of acidity. We examined these parameters in producers’ and commercial samples. We succeed fund parameters which characteristic of one type of honey thus we have possibility identify of honey type. For example the proline content was very low in the producers’ linden honey (mean 126 mg/kg). This value is lower than the regulation, but in case of commercial linden honey this parameter is higher than regulation. According to electrical conductivity and Potassium content is provable the type of linden honey. -
Qualitative detection of genetically modified organisms in plant samples
309-313Views:177We analysed the GMO content of corn samples by polymerase chain reaction following the appropriate optimization of the reaction. The analysis included two main steps: extraction of DNA from the sample, and detection of the GMO content by polymerase chain reaction. The polymerase chain reaction is an in vitro method to multiply chromosomatic or cloned DNA (cDNA) sequences through the enzymatic pathway. The reaction is sensitive enough to produce DNA in sufficient amount for the analysis from a single DNA. We identified the PCR products by agarose gel electrophoresis. When optimizing the reaction, the MgCl2 concentration, reaction time and temperature have to be taken into consideration. The temperature of the anellation has to be increased until the highest specificity and yield is reached. If the temperature of the anellation is too high, the primer is linked to non-specific sites as well; in the gel visualization, more lines can be seen at one sample. If the temperature of the anellation is too high, the primer is insufficiently linked or is not linked at all (too few lines in the gel visualization). After optimization, the GMO content in the unknown sample can be determined along with the appropriate positive and negative controls.